Integrative transcriptomic, metabolomic, and physiological analyses reveal the role of strigolactones in enhancing drought resistance in Juglans sigillata

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-09-14 DOI:10.1016/j.plaphy.2025.110529

Qing Shang , Zhaoyang Shi , Zhangjun Wei , Shiwei Li , Lei Fang , Dong Huang

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引用次数: 0

Abstract

Juglans sigillata, an economically valuable tree species mainly cultivated in southwest China, is frequently affected by seasonal drought, which significantly impacts its growth and yield. This study investigated the role of strigolactones (SLs) in mitigating drought stress through physiological, transcriptomic, and metabolomic analyses, along with overexpression of SLs synthesis-related gene JsCCD7 in Arabidopsis thaliana. Exogenous SLs spraying effectively alleviated drought adverse effects: SPAD value and relative water contents of walnut leaves increased by 47.3 % and 13.9 %, respectively; net photosynthetic rate, stomatal conductance, and transpiration rate rose by 67.5 %, 51.2 %, and 61 %, respectively; whereas malondialdehyde, hydrogen peroxide, and superoxide anion contents decreased by 9.8 %, 17 %, and 11 %, respectively. High-throughput sequencing revealed that SLs enhanced drought resistance by upregulating lignin synthesis genes and their precursors (caffeic acid and Coniferin), as well as elevating rutin and quercetin levels in the flavonoid pathway to alleviate oxidative damage and stress response. Additionally, three Arabidopsis lines overexpressing JsCCD7 exhibited higher SLs content, total flavonoid levels, and biomass compared to wild-type under drought conditions, along with reduced reactive oxygen species damage. In summary, SLs enhanced drought resistance and tolerance, alleviated stress, and promoted growth in J. sigillata seedlings. This study provides a theoretical basis for improving drought resistance in J. sigillata through SLs-related pathways.

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综合转录组学、代谢组学和生理学分析揭示了独角麦内酯在提高核桃抗旱性中的作用。

摘要sigillata是中国西南地区重要的经济树种，季节性干旱是影响其生长和产量的重要因素。本研究通过生理、转录组学和代谢组学分析，以及拟南芥中独角麦内酯合成相关基因JsCCD7的过表达，研究了独角麦内酯（SLs）在缓解干旱胁迫中的作用。外源SLs喷洒有效缓解了干旱的不利影响，核桃叶片SPAD值和相对含水量分别提高了47.3%和13.9%；净光合速率、气孔导度和蒸腾速率分别提高67.5%、51.2%和61%；而丙二醛、过氧化氢和超氧阴离子含量分别下降了9.8%、17%和11%。高通量测序结果显示，SLs通过上调木质素合成基因及其前体（咖啡酸和松柏苷），以及提高黄酮类通路中的芦丁和槲皮素水平来增强抗旱性，从而减轻氧化损伤和应激反应。此外，与野生型相比，3个过表达JsCCD7的拟南芥品系在干旱条件下表现出更高的SLs含量、总黄酮水平和生物量，并减少了活性氧的伤害。综上所述，SLs提高了木质素幼苗的抗旱性和耐旱性，缓解了胁迫，促进了木质素幼苗的生长。本研究为通过sls相关途径提高木质素抗旱性提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.