Plant Physiology and Biochemistry最新文献

{"title":"Encapsulation of melatonin in pH-responsive β-CD nanosponges for enhanced stability and optimized delivery in agriculture","authors":"Parul Sangwan,&nbsp;Vinita Hooda","doi":"10.1016/j.plaphy.2025.110148","DOIUrl":"10.1016/j.plaphy.2025.110148","url":null,"abstract":"<div><div>The current study focuses on the development of a pH-responsive nanosponge designed to enhance the stability and controlled release of phytohormone melatonin. The nanosponge, characterized by disulfide bridges and a stabilizer, allows for regulated release in acidic conditions typical of plant rhizospheres. Four different melatonin to nanosponge weight ratios (1:1, 2:1, 3:1 and 4:1) were prepared and analyzed using Ultra Performance Liquid Chromatography to quantify melatonin loading. The optimized ratio (3:1) was characterized by various techniques, including Zeta potential analysis, FESEM-EDX, FTIR, XRD and DSC. <em>In vitro</em> release studies and photostability tests confirmed the effectiveness of nanosponge in controlled melatonin release and enhanced photostability. Melatonin-loaded nanosponges significantly alleviated salinity stress in tomato plants, enhancing yield and reducing the frequency and dosage of melatonin application. This innovative approach promises improved melatonin stability and efficient agricultural use, offering a novel method to mitigate abiotic stress in plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110148"},"PeriodicalIF":6.1,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Starch-sugar metabolism in response to wound stress in apple fruit","authors":"Huiwen Deng ,&nbsp;Pengdong Xie ,&nbsp;Yong Han ,&nbsp;Xi Song ,&nbsp;Yonghong Ge","doi":"10.1016/j.plaphy.2025.110142","DOIUrl":"10.1016/j.plaphy.2025.110142","url":null,"abstract":"<div><div>The sugar molecule is essential for plant response to abiotic stress. Leveraging previously obtained transcriptome data, this study investigated the changes in starch and cellulose degradation as well as sucrose metabolism in post-harvest mechanically wounded apples. The findings demonstrated that wounding up-regulated the expression levels of <em>MdAMY1.1</em>, <em>MdAMY2</em>, <em>MdBAM1</em>, and <em>MdBAM3</em>, which in turn enhanced the enzymatic activities of <em>α</em>-amylase (AMY) and <em>β</em>-amylase (BMY), thereby accelerating the degradation of total starch, amylose, and amylopectin. Conversely, it down-regulated the expression levels of <em>MdSS3</em> and <em>MdSS4</em>, reduced the enzymatic activities of sucrose synthase synthesis (SS-s) and sucrose synthase cleavage (SS-c). Wounding also up-regulated the expression levels of cellulase and <em>β</em>-glucosidase genes, thus facilitating the degradation of cellulose. Moreover, wound stress induced the up-regulation of <em>MdSPS1</em>, <em>MdCWINV1</em>, <em>MdINV∗DC4</em>, and <em>MdTIV1</em> expression levels, thereby maintaining the balance of sucrose metabolism. Consequently, this leads to an increase in the biosynthesis of glucose, fructose, sucrose, and reducing sugars at the wound sites. All these evidences indicate that starch and cellulose at wound sites are degraded to produce sugars, which facilitate the wound healing process in apple fruit, thereby helping to reduce microbial infections and minimize post-harvest losses.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110142"},"PeriodicalIF":6.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144239412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of monochromatic light on growth and development of Phoebe bournei seedlings tend to be \"extreme\"","authors":"Shicheng Su ,&nbsp;Nianqing Jin ,&nbsp;Ruixin Nie ,&nbsp;Xiaoli Wei","doi":"10.1016/j.plaphy.2025.110116","DOIUrl":"10.1016/j.plaphy.2025.110116","url":null,"abstract":"<div><div>Light quality is a key factor affecting seedling regeneration in natural <em>Phoebe bournei</em> forests. We used the current-year <em>P</em>. <em>bournei</em> seedlings as experimental materials and cultured them under five LED spectral conditions for 60 days to explore the response mechanism of <em>P</em>. <em>bournei</em> seedlings to different light qualities (blue, red, green, blue-red (1:1) and white (control)). We analyzed the impact of monochromatic lights and blue-red mixed light on leaf structure, starch, soluble sugar, soluble protein, root development, and seedling quality of <em>P</em>. <em>bournei</em> seedlings. The effects of monochromatic light on the development of <em>P. bournei</em> seedlings tended to be \"extreme\". Red and green light increased starch grain size by 2.12 and 2.03 times compared to white light. The excessive accumulation of starch grains disrupted the ultrastructure of chloroplasts, inhibited the synthesis and utilization of carbohydrates, and thus limited the development of seedlings. Blue light accelerated the transport and utilization of carbohydrates, significantly improved the development of chloroplast ultrastructure, and activated the rapid growth of seedlings. Especially, blue light promoted the formation of lateral roots, and significantly increased leaf area, leaf thickness, and stomatal density. However, the allocation of stem biomass was reduced under blue light, resulting in imbalanced inter-organ development and decreased seedling resistance. In contrast, under the blue-red light, the chloroplast development state, the relationship between storage and utilization of starch and other substances, as well as carbon and nitrogen metabolic activities in seedlings were more coordinated, indicating that multispectral light has the potential to cultivate <em>P. bournei</em> seedlings of superior quality. Although the seedling quality index (SQI) of <em>P. bournei</em> under the blue-red light in this experiment did not reach its best, our findings provide a valuable theoretical basis of high-quality <em>P. bournei</em> seedlings and for regulating the light environment when planting <em>P. bournei</em> under the forest canopy.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110116"},"PeriodicalIF":6.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ELF18-INDUCED LONG NONCODING RNA 11 negatively regulates the innate immune response by suppressing Pathogenesis-related Lipase 5","authors":"Jimin Lee ,&nbsp;Nuri Oh ,&nbsp;Moonhyuk Kwon ,&nbsp;Jun Sung Seo ,&nbsp;Jang-Kyun Seo ,&nbsp;Jin-Ho Kang ,&nbsp;Hye Sun Cho ,&nbsp;Choonkyun Jung","doi":"10.1016/j.plaphy.2025.110138","DOIUrl":"10.1016/j.plaphy.2025.110138","url":null,"abstract":"<div><div>Long noncoding RNAs (lncRNAs) are essential components of diverse biological processes. However, lncRNAs involved in the innate immune response remain poorly unknown in plants. Here, we isolated ten lncRNAs named <em>ELF18-INDUCED LONG NONCODING RNA</em> (<em>ELENA2</em> to <em>11</em>) from custom lncRNA array datasets generated from <em>Arabidopsis</em> seedlings treated with pathogen-associated molecular patterns (PAMPs; elf18 and flg22). Each <em>ELENA</em> exhibited different expression patterns in a tissue- and PAMP-specific manner. <em>ELENA11</em> is an intergenic lncRNA that is located between AT5G24170 and <em>Pathogenesis-Related Lipase 5</em> (<em>PRLIP5</em>; AT5G24180). <em>ELENA11</em>-overexpressing plants showed reduced <em>PRLIP5</em> expression and callose deposition compared to wild-type plants under elf18 and flg22 treatments, whereas <em>ELENA11</em> knockout plants showed enhanced <em>PRLIP5</em> expression and callose deposition, indicating that <em>ELENA11</em> acts as a negative regulator of <em>PRLIP5</em> expression. In addition, <em>ELENA11</em>-overexpressing plants were more susceptible to disease caused by <em>Pseudomonas syringae</em> pv. <em>tomato</em> DC3000 than wild-type plants, whereas <em>ELENA11</em> knockout plants were resistant. These results demonstrate that <em>ELENA11</em> plays a distinct role in PAMP-triggered innate immune responses by repressing <em>PRLIP5</em> expression.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110138"},"PeriodicalIF":6.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144239413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated genomic, transcriptomic, and metabolomic analyses provide novel insights into high cadmium accumulation in Phytolacca icosandra L","authors":"Xiong Li ,&nbsp;Tao Jin ,&nbsp;Yanxia Jia ,&nbsp;Dongmei Yu ,&nbsp;Juncheng Liu ,&nbsp;Na Hu ,&nbsp;Yanshuang Li ,&nbsp;Boqun Li ,&nbsp;Guangyan Wang ,&nbsp;Guihua Jin ,&nbsp;Ngueuyim Nono Josy Karel ,&nbsp;Jianchu Xu","doi":"10.1016/j.plaphy.2025.110140","DOIUrl":"10.1016/j.plaphy.2025.110140","url":null,"abstract":"<div><div>The evolutionary features and molecular basis of the conserved abilities of <em>Phytolacca</em> species to accumulate cadmium (Cd) and manganese (Mn) are poorly understood. In this study, the chromosomal-level genome of <em>P. icosandra</em> (2n = 2x = 36) was sequenced and assembled. The genome has a size of 1042.86 Mb, with a contig N50 of 52.37 Mb and a GC content of 38.17%. Genomic evaluation revealed that the <em>P. icosandra</em> genome contains 98.95% of BUSCO and achieves an LAI score of 15.45. Genome annotation indicated that the <em>P. icosandra</em> genome encompasses 25,756 protein-coding genes. Evolutionary analysis identified one recent whole genome duplication event and showed that the unique, significantly expanded, and positive selection genes in <em>P. icosandra</em> were markedly enriched in various functional categories (e.g., zinc ion binding, Mn ion binding, nicotianamine biosynthetic process) associated with metal (e.g., Cd and Mn) responses. Furthermore, several of these evolution-related genes were significantly upregulated by 25 and 100 μM Cd stress. Among them, an expanded gene <em>PiNAS-l</em> significantly improved Cd tolerance in the <em>Δycf1</em> yeast strain. These results indicate that the high-Cd-accumulating characteristics of <em>P. icosandra</em> may be driven by evolutionary selection. Integrated physiological, transcriptomic, and metabolomic analyses revealed that several key processes, including Cd transport, signal transduction, lignin- and flavonoid-biosynthesis, and carbohydrate metabolism, contributed to Cd detoxification in <em>P. icosandra</em> roots. These processes were likely regulated by multiple transcription factor networks involving in WRKY, ERF, MYB, bHLH, bZIP, and several other families. This study provides the first chromosomal-level genome of Phytolaccaceae species and offers novel insights into Cd tolerance and accumulation in plants from evolutionary and molecular perspectives.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110140"},"PeriodicalIF":6.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ectopic expression of strawberry FaMADS6 represses anthocyanin biosynthesis in both tobacco and Arabidopsis thaliana","authors":"Yang Zhang ,&nbsp;Jie Zhou ,&nbsp;Weiwen Qiu ,&nbsp;Yanan Sun ,&nbsp;Yupeng Hu ,&nbsp;Jing Zhao ,&nbsp;Congbing Fang ,&nbsp;Xingbin Xie","doi":"10.1016/j.plaphy.2025.110139","DOIUrl":"10.1016/j.plaphy.2025.110139","url":null,"abstract":"<div><div>Anthocyanins are important secondary metabolism phytochemicals that contribute to health, quality, and plant stress resistance. The class B MADS-box gene family is fundamental for floral organ development across different species. In this study, we reveal a novel function of the strawberry <em>PISTILLATA</em> homologue <em>FaMADS6</em>, in <em>N. tabacum</em> and <em>Arabidopsis</em>. <em>FaMADS6</em> overexpression in tobacco affects floral organ development and seed set, leading to changes in pistil stigma, stamen petalisation and corolla morphology. Interestingly, these phenotypic alternations were not observed in transgenic <em>Arabidopsis</em>. We investigated the connection between FaMADS6 and anthocyanin accumulation. <em>FaMADS6</em> expression levels in red- and white-petaled strawberries were negatively correlated with anthocyanin accumulation. Overexpression of <em>FaMADS6</em> repressed anthocyanin production, as well as the transcript levels of structural genes and key R2R3 MYB-family transcription factors in transgenic tobacco petals and <em>Arabidopsis</em> seedlings. Additionally, Y1H, EMSA, and Dual-luc assays demonstrated that <em>FaMADS6</em> regulated the transcription of <em>NtAN2</em>, <em>AtMYB75</em>, and <em>AtMYB9</em>0 by directly binding to their promoter regions, leading to the repression of their expression. Furthermore, exogenous ABA at varying concentrations inhibited the promoter activity of <em>FaMADS6</em>, highlighting the role of ABA-mediated repression of <em>FaMADS6</em> in regulating anthocyanin biosynthesis during strawberry fruit development. Therefore, as a B-functional gene, <em>FaMADS6</em> is involved not only in determining the identity of floral organs but also in repressing anthocyanin biosynthesis. Overall, this study deepens our understanding of the multifunctional evolution of plant class B MADS-box genes and provides molecular insights that could contribute to improving strawberry quality.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110139"},"PeriodicalIF":6.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichoderma atroviride enhances ROS scavenging systems, primary and secondary metabolism increasing the yield of soybean plants","authors":"Cleverson Rodrigues ,&nbsp;Nandhara Angelica Carvalho Mendes ,&nbsp;Pedro Henrique Gorni ,&nbsp;Grace Queiroz David Peres ,&nbsp;André Rodrigues dos Reis","doi":"10.1016/j.plaphy.2025.110143","DOIUrl":"10.1016/j.plaphy.2025.110143","url":null,"abstract":"<div><div>Trichoderma application offers soybean farmers global benefits by enhancing plant growth stimulation and crop yield. Globally, beneficial fungus application rise as more sustainable agricultural practices, improving resilience against climate change impacts. Biostimulants are promising tools for mitigating abiotic stresses and increasing soybean yield. Trichoderma has emerged as an innovative approach for sustainable agricultural solutions. Microorganisms that enhance agricultural production show several important traits in bioprospecting. This study aimed to evaluate the biostimulant effect of Trichoderma isolates on the growth, physiological performance, and yield of soybean plants. Five fungal isolates of <em>Trichoderma atroviride</em> were used. Trichoderma was inoculated as colonized rice incorporated into the soil before soybean sowing, and also applied as a foliar application of conidial suspension at the V₃ stage. Parameters related to plant growth, antioxidant and secondary metabolism were determined. The application of isolates dramatically increased the concentration of glyceollin (main phytoalexin) in soybean cotyledons. In addition, all Trichoderma isolates elicited the activity of plant defense-inducing enzymes (POD, PPO, LOX, PAL, chitinase, and β-1,3-glucanase) and antioxidant enzymes (SOD, CAT, and APX) in soybean leaves. The Trichoderma isolates promoted a biostimulant effect increasing shoot and root biomass, and nodulation of soybean plants. Increased nodulation, antioxidant activity, and secondary metabolism resulted in enhanced soybean plant growth, leading to higher globulin concentration in the seeds. These phenomena resulted in increased seed mass and yield of soybean plants. These results suggest the use of Trichoderma for more sustainable agriculture show a great potential to increase soybean yield under field conditions worldwide.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110143"},"PeriodicalIF":6.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen demand disparities and physiological responses in grapevine cultivars under contrasting nitrogen regimes","authors":"Yuxia Wu ,&nbsp;Zedong Sun ,&nbsp;Rui Zhang ,&nbsp;Yanxiu Wang","doi":"10.1016/j.plaphy.2025.110146","DOIUrl":"10.1016/j.plaphy.2025.110146","url":null,"abstract":"<div><div>Nitrogen assimilation critically regulates plant developmental plasticity and metabolic homeostasis. Although intraspecific nitrogen demand variation influences agricultural efficiency, its systematic characterization in grapevines remains limited. This study elucidated cultivar-specific nitrogen responsiveness through Comparative growth-physiological analysis of two phenotypically distinct <em>Vitis vinifera</em> cultivars (‘Red Globe’ and ‘Crimson Seedless') under five controlled nitrogen regimes (3.34, 6.68, 13.36, 20.04, and 26.72 mM). The findings indicated that nitrogen levels play a crucial role in regulating the growth and physiological processes of two grapevine cultivars. The ‘Red Globe’ demonstrated optimal growth at a nitrogen concentration of 20.04 mM, while the ‘Crimson Seedless' reached its peak performance at 13.36 mM, reflecting a 33.3 % lower nitrogen requirement compared to ‘Red Globe’. Both cultivars experienced growth inhibition and impaired physiological traits under conditions of both nitrogen deficiency and excess. At their respective optimal nitrogen concentrations, both cultivars accumulated significant biomass in aboveground tissues and roots, maintained elevated chlorophyll levels in leaves to maximize photosynthetic capacity, and enhanced protective enzyme activities (e.g. SOD, POD, CAT), along with osmolyte accumulation. These adaptations strengthened antioxidant defenses and sustained physiological functionality. Gene expression analysis revealed that key nitrogen metabolism genes in ‘Crimson Seedless' (<em>VvNR1/2</em>, <em>VvGDH</em>, <em>VvNiR</em>) were upregulated with increasing nitrogen supply but were rapidly downregulated under conditions of nitrogen excess, indicating more rapid adaptive metabolic responses. Comparative analysis revealed that ‘Crimson Seedless' exhibited heightened sensitivity to nitrogen, whereas ‘Red Globe’ showed greater tolerance to nitrogen variations. These findings offer significant insights for the precision management of nitrogen in grape cultivation.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110146"},"PeriodicalIF":6.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere ecology and physiological regulation drive cadmium accumulation efficiency in Hylotelephium spectabile populations","authors":"Junmei Guo ,&nbsp;Bingxin Guo ,&nbsp;Junxing Yang ,&nbsp;Xiaona Liu ,&nbsp;Tianwei Qian ,&nbsp;Yuexing Wei ,&nbsp;Yuan Jiao ,&nbsp;Tongbin Chen","doi":"10.1016/j.plaphy.2025.110141","DOIUrl":"10.1016/j.plaphy.2025.110141","url":null,"abstract":"<div><div>Understanding the rhizo-ecological characteristics and physiological responses to Cd stress provides insight into the intrinsic Cd accumulation mechanisms of <em>Hylotelephium spectabile</em>. Under Cd exposure, the LN population exhibited a growth advantage over HB, attributed to Cd-induced chlorophyll enhancement. LN showed significantly increased root secretion of acetic and malic acids under Cd stress, acidifying the rhizosphere and providing functional groups for Cd-OA complex formation. Consequently, water-soluble and acid-extractable Cd levels were higher in the LN rhizosphere than in the HB rhizosphere. The increased proportion of protein-integrated Cd and decreased phosphate-sequestered Cd in the stems, along with the opposite trend in the leaves, revealed a key mechanism for efficient Cd translocation in the LN. Rhizosphere urease and dehydrogenase activities in LN responded more positively to Cd stress than in HB, providing improved nutrient conditions for growth. Furthermore, Cd-induced Proteobacteria and Chloroflexi enrichment in the LN rhizosphere played essential roles in modulating root exudates, pH, and Cd bioavailability. Upregulated pathways related to lipid/amino acid metabolism, as well as genetic replication and repair, may underlie the superior Cd accumulation efficiency of LN compared with HB.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110141"},"PeriodicalIF":6.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The L-type lectin receptor-like kinase GmLecRK-IX negatively regulates drought stress responses in soybean seedlings","authors":"Yubin Wang ,&nbsp;Rongfan Zheng ,&nbsp;Ran Xu ,&nbsp;Caijie Wang ,&nbsp;Wei Liu ,&nbsp;Wei Li ,&nbsp;Zhenwu Nan ,&nbsp;Haiying Dai ,&nbsp;Yanwei Zhang ,&nbsp;Lifeng Zhang","doi":"10.1016/j.plaphy.2025.110134","DOIUrl":"10.1016/j.plaphy.2025.110134","url":null,"abstract":"<div><div>Soybean (<em>Glycine max (L.)</em> Merr.), a prominent oilseed crop grown around the world, provides essential resources for the human diet as well as livestock feed. However, drought stress severely impacts soybean yield and quality. The presence of lectin receptor-like kinases (LecRLKs) has been identified as being pivotal to plant development and the manifestation of responses to environmental stresses. Nevertheless, there is a paucity of literature on the involvement of LecRLKs in the response of soybean to drought conditions. In this investigation, we discovered an L-type soybean <em>LecRK-IX</em> gene (Glyma.09G150400) using transcriptomic and proteomic approaches. Quantitative reverse transcription PCR analysis revealed that drought stress significantly elevated <em>GmLecRK-IX</em> gene expression. Subcellular localization results indicated that GmLecRK-IX was localized to the endoplasmic reticulum (ER) and the plasma membrane of <em>Nicotiana benthamiana</em> leaves and Arabidopsis protoplasts. Expression pattern analysis revealed that <em>GmLecRK-IX</em> was predominantly expressed in the roots and seeds. Transgenic Arabidopsis plants overexpressing the <em>GmLecRK-IX</em> gene displayed heightened sensitivity to drought, as evidenced by a decreased survival rate, reduced proline content, and elevated malondialdehyde (MDA) levels following drought stress treatment. Furthermore, the transgenic <em>GmLecRK-IX</em> overexpressed (-OE) lines of soybean exhibited a negative effect on the capacity to withstand drought conditions. The transgenic soybean <em>GmLecRK-IX</em>-OE lines exhibited lower levels of leaf water and proline, a higher rate of water loss, increased MDA content, and reduced activities of antioxidant enzymes. RNA-sequencing analysis further revealed that the <em>GmLecRK-IX</em>-OE lines exhibited altered expression of the genes encoding peroxidase (<em>PRXs</em>) and plasma membrane intrinsic proteins (<em>PIPs</em>) in soybean under drought conditions. Our research has uncovered a novel regulatory mechanism in soybean under drought stress and identified potential target genes for development drought-tolerant soybean varieties.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110134"},"PeriodicalIF":6.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}