Plant Physiology and Biochemistry最新文献

{"title":"The inhibition effect of high temperature stress on potato tuber skin coloring mainly occurred in the belowground part of the plant.","authors":"Jinhua Zhou, Kaifeng Li, Maoxing Li, Youhan Li, Huachun Guo","doi":"10.1016/j.plaphy.2024.109407","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109407","url":null,"abstract":"<p><p>High temperature stress leads to a dramatic reduction of both the anthocyanin concentration and the appearance quality of colored potatoes. However, it remains uncertain if the high temperature impacts potato tuber skin coloring through only the aerial or belowground parts of the plant, or through their interaction; and it's underlying reason is still unclear. In this study, the red-skin cultivar Qingshu9 (Qs9) was exposed to the high-temperature (30 °C) treatment on the belowground part alone (BH), aerial part alone (AH) and entire plant (EH), and the normal-temperature treatment on entire plant (EN) as control. The results indicated that the total anthocyanin content in tuber skin of the BH treatment was significantly lower than the EN and AH treatment, and there was no accumulation of cyanidin and pelargonidin in BH treatment, only peonidin. Compared with the EN treatment, the decrease rate of total anthocyanin content of the AH treatment was much smaller than the BH treatment, and the composition of anthocyanin did not change. Transcriptome analysis showed the downregulated DEGs of BH vs EN, BH vs AH and AH vs EN were significantly associated with the anthocyanin synthesis and metabolism pathway. High temperature inhibited anthocyanin synthesis by reducing the expression of key genes (StPAL, StF3H, StF3'H, StF3'5'H, StDFR and StANS) in the anthocyanin synthesis pathway. In summary, high temperature inhibits anthocyanin synthesis in tuber skin by downregulating key genes, and this inhibitory effect mainly occurs through the belowground part of the plant.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109407"},"PeriodicalIF":6.1,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome analysis and functional verification revealed that GhSAP6 negatively regulates salt tolerance in upland cotton.","authors":"Zhen Peng, Abdul Rehman, Xuran Jiang, Chunyan Tian, Zhenzhen Wang, Hongge Li, Xiaoyang Wang, Adeel Ahmad, Muhammad Tehseen Azhar, Xiongming Du, Shoupu He","doi":"10.1016/j.plaphy.2024.109406","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109406","url":null,"abstract":"<p><p>Owing to the scarcity of cultivable land in China, the agricultural sector is primarily focused on grain and oil crops. Simultaneously, the cultivation of cotton has gradually shifted towards regions characterized by elevated soil salinity levels. Additionally, the mechanism behind cotton's ability to tolerate salt remains elusive. In this study, we identified the Z9807 genotype as highly tolerant to salt stress, exhibiting superior leaf wilting resistance, antioxidant activity, catalase activity, K⁺/Na⁺ ratio, and growth compared to the salt-sensitive ZJ0102. Comparative transcriptome analysis revealed marked differences in salt stress responses between Z9807 and ZJ0102. This study identified a considerable number of differentially expressed genes associated with salt tolerance across multiple time points. By integration of QTL and GWAS mapping data, we successfully identified 621 candidate genes associated with salt tolerance. Weighted gene correlation network analysis exhibited three co-expression modules related to salt-tolerant Z9807 samples, ultimately identifying 15 core salt-tolerant candidate genes. We also conducted in-depth research on the salt tolerance of the stress-associated protein (SAP) GhSAP6 (GhSAP6_At and GhSAP6_Dt homologs). Results revealed that these candidate genes may inhibit salt tolerance through Virus-Induced Gene Silencing (VIGS) and transgenic overexpression assays conducted in Arabidopsis thaliana. Furthermore, we used yeast two-hybrid and luciferase assay experiments to confirm the ubiquitin degradation pathway between selected interacting proteins and verified the interaction with RAD23C. This study will provide new insights into the mechanisms related to salt tolerance in upland cotton.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109406"},"PeriodicalIF":6.1,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of tree peony under high temperature treatment and functional verification of PsDREB2A gene.","authors":"Wenxuan Bu, Yu Huang, Lujie Chen, Minhuan Zhang, Xiaoning Luo, Tangchun Zheng, Fengxia Shao, Weiqun Lei, Wen Xing, Xingyu Yang, Bowen Wang, Zheng Wang","doi":"10.1016/j.plaphy.2024.109405","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109405","url":null,"abstract":"<p><p>Paeonia suffruticosa is a plant of Paeonia in Paeoniaceae. It is an important woody ornamental flower in the world. High temperature in summer hinders the growth of tree peony and reduces its ornamental quality, which restricts the cultivation and application of tree peony in Jiangnan area of China. Paeonia suffruticosa 'Hu Hong' is a traditional Chinese tree peony variety with high ornamental value. It is an excellent parent material for cultivating heat-resistant peony. This paper selected the tree peony variety 'Hu Hong' as the material. The transcriptome data of Paeonia suffruticosa 'Hu Hong' at 0, 2, 6, 12 and 24 h after high temperature treatment were analyzed by RNA-Seq method. At each time point, a large number of significantly differentially expressed genes(DEGs) were screened between tree peony cultured at high temperature and room temperature. The analysis of the common DEGs in the four comparison groups showed that the differential genes were mainly enriched in the GO terms ' protein processing in endoplasmic reticulum', 'Pentose and glucuronate interconversions ', ' plant-pathogen interaction ', ' zeatin biosynthesis ', ' fatty acid elongation ', and ' plant hormone signal transduction ' pathways. Abscisic acid(ABA), ethylene(ET) and brassinosteroid(BR) signaling related genes were significantly up-regulated in 'Hu Hong' to resist high temperature treatment. In the auxin(IAA), cytokinin(CTK), gibberellin(GA), salicylic acid(SA) pathways, compared with the control group, the down-regulated expression was involved in hormone signal transduction to respond to high temperature treatment. A total of 62 TFs from 28 different families were annotated, with the AP2/ERF family annotating the largest number. Among the TFs annotated to the AP2/ERF family, the highest expression gene PsDREB2A was found. Overexpression of PsDREB2A Arabidopsis plants improved heat tolerance under high temperature treatment. However, silencing PsDREB2A in tree peony resulted in a heat-intolerant phenotype. PsDREB2A can directly bind to the DRE-core motif in the PsHSFA3 promoter to initiate its expression. In addition, PsHSFA3-overexpressing plants showed higher heat resistance, while PsHSFA3-silenced plants showed lower heat resistance. This study provides a scientific basis for in-depth study of the molecular mechanism of high temperature treatment response in tree peony, improving the heat signal transduction regulation network of tree peony, and mining heat-resistant related genes.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109405"},"PeriodicalIF":6.1,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A genus-specific R2R3 MYB transcription factor, CsMYB34, regulates galloylated catechin biosynthesis in Camellia sinensis.","authors":"Jianmei Xu, Jingyi Li, Yihao Liu, Peng Zheng, Shaoqun Liu, Binmei Sun","doi":"10.1016/j.plaphy.2024.109401","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109401","url":null,"abstract":"<p><p>Galloylated catechins are the dominant polyphenols in Camellia sinensis (L.) O. Kuntze. The mechanisms responsible for accumulation of these specialized metabolites in tea plants remains unclear. This paper presents an extended member of subgroup 5 of transcription factors R2R3-MYB, CsMYB34, as a critical gene specifically regulating galloylated catechin biosynthesis. CsMYB34 has a TT2-type motif [VIRTKATRCSKVFIP]. Its transcription levels were positively correlated with galloylated catechin content in 19 tea varieties, with correlation coefficients ≥0.79. Suppression of CsMYB34 expression caused a significant decrease in galloylated catechin content, as well as reduced expression levels of the key galloylated catechin biosynthesis gene CsSCPL4. Yeast one-hybrid (Y1H), electrophoretic mobile shift assay (EMSA) and dual-luciferase reporter system (DLR) showed that CsMYB34 interacts directly with the promoter region of CsSCPL4, thereby upregulating its transcription. This research indicates that the CsMYB34 transcription factor selectively modulates the biosynthetic pathway of galloylated catechins, thereby offering a plausible rationale for the observed elevated levels of these compounds in tea leaves.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109401"},"PeriodicalIF":6.1,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1+1<2: Combined effect of low temperature stress and salt stress on Sesuvium portulacastrum L.","authors":"Wei Liu, Jinlin Liu, Meijing Zhang, Jianlin Zhang, Bin Sun, Chiquan He, Peimin He, Wentao Zhang","doi":"10.1016/j.plaphy.2024.109404","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109404","url":null,"abstract":"<p><p>To expedite the deployment of Sesuvium portulacastrum floating bed technology in Hangzhou Bay and the Yangtze River Estuary, and to overcome the cryogenic constraint, our study concentrated on investigating the impacts of both individual and combined stress factors, particularly low temperature and salinity, on its application. We detected the S. portulacastrum related enzyme activity and other biological macromolecules under low temperature stress, salt stress and combined stress. And we also analyzed the stress resistance mechanism under different stress conditions by transcriptomic technology. It was discovered that moderate salt stress could enhance plant tolerance to low temperature, indicating the presence of an antagonistic relationship between salinity and low temperature. The biological mechanism underlying this phenomenon lies in the fact that combined stresses induce the up-regulation of various genes and activate more pathways compared to single stress. Among these pathways, the linoleic acid metabolic pathway stands out as unique to combined stress conditions. This research represents the inaugural endeavor to investigate the impact of low temperature stress and combined stress on S.portulacastrum, offering a pivotal reference for the utilization of this plant in ecological restoration and management within the East China Sea. More valuable is that such conclusions may be extended to the coastal ecological governance of many high latitude countries, which is of great significance for global ecological environment improvement.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109404"},"PeriodicalIF":6.1,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The essential role of the hickory StMADS11 subfamily in flower organogenesis and flowering time in Arabidopsis.","authors":"Caiyun Li, Zhengfu Yang, Zhichao Sun, Di Wu, Bo Zhang, Hongmiao Jin, Kean-Jin Lim, Zhengjia Wang","doi":"10.1016/j.plaphy.2024.109402","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109402","url":null,"abstract":"<p><p>The StMADS11 subfamily genes play a crucial role in regulating flowering time, flower development, and bud dormancy in plants. These genes exhibit functional differences between annual and perennial woody plants. In hickory (Carya cathayensis Sarg.), the specific roles of these genes in flowering regulation have not been elucidated. In this study, we identified five StMADS11 subfamily genes in the hickory genome, designated as CcSVP-like, CcAGL24-like1, CcAGL24-like2, CcJOINTLESS-like1, and CcJOINTLESS-like2, based on their clustering characteristics. Sequence analyses revealed distinct structural features in this subfamily, including differences in intron length, C domain, and conserved motifs. Transcript analysis indicated high expression levels of these genes in female flower buds, along with a notable seasonal expression pattern. Overexpression studies on Arabidopsis have demonstrated that the StMADS11 subfamily genes lead to various floral organ and pod anomalies. Specifically, overexpression of CcSVP-like resulted in delayed flowering, while overexpression of CcAGL24-like1, CcAGL24-like2, CcJOINTLESS-like1, and CcJOINTLESS-like2 promoted flowering. Protein interaction studies have shown that the StMADS11 subfamily proteins bind to the CcFUL-like protein. Notably, CcFUL-like, CcSVP-like, CcJOINTLESS-like1, and CcJOINTLESS-like2 proteins were able to bind to the CcSOC1-like promoter and suppress its expression. Our findings elucidate the distinct roles of the StMADS11 subfamily genes in flower development and timing, contribute to developing the current understanding of flowering regulation in hickory, and offer a foundation for further studies in perennial woody plants.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109402"},"PeriodicalIF":6.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142838473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation into the mechanisms of photosynthetic regulation and adaptation under salt stress in lavender.","authors":"Ling Li, Yinan Liu, Yujing Jia, Zening Yuan","doi":"10.1016/j.plaphy.2024.109376","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109376","url":null,"abstract":"<p><p>Salinity stress is a major threat to agricultural productivity and sustainability, often causing irreversible damage to photosynthesis. Lavender, a valuable aromatic plant, experiences growth impacts under salt stress. However, the regulatory mechanisms of photosynthesis related to its adaptation to salt stress remain unclear. In this study, lavender was exposed to 0, 100, 200, and 300 mM NaCl for 28 days. It was observed that lavender effectively maintained chlorophyll stability when salt concentrations were below 200 mM and stress duration was under 21 days. The most effective model for lavender under salt stress was identified as a right-angled hyperbolic modified model. Under moderate salt stress (100 mM, 200 mM), genes such as LaPSB28, LaPSBS, and LaPSBR contributed to PSII core stability, enhanced photosynthetic pigment levels, and sustained high electron transfer rates to improve salt-tolerance. Additionally, LaLHCB4-1 and LaPSAK-1 regulated stomatal size, thereby facilitating gas exchange and supporting the photosynthetic process. Conversely, under high salt stress (300 mM), LaPSBW-1, -2, and LaPSAB were found to reduce photosynthetic pigment levels and inhibit photosynthetic activity. However, genes such as LaCHLG-2, LaGLG-3, LaBAM1-1 and -3, and LaCHLP-3 aided in starch synthesis by increasing pigment content, thus promoting energy balance and enhancing salt tolerance. This regulation involved photosynthesis-antenna proteins and pathways related to starch, sucrose, and chlorophyll metabolism. These findings may support the cultivation of salt-tolerant lavender varieties and maximize saline soil usage.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109376"},"PeriodicalIF":6.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BcWRKY53 promotes chlorophyll biosynthesis and cold tolerance of non-heading Chinese cabbage under cold stress.","authors":"Xiaoshan Chen, Zhanyuan Gao, Zhanghong Yu, Qiang Ding, Xiaojun Qian, Chenyang Zhang, Chenyu Zhu, Yaolong Wang, Changwei Zhang, Ying Li, Xilin Hou","doi":"10.1016/j.plaphy.2024.109398","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109398","url":null,"abstract":"<p><p>WRKY transcription factors are widely involved in plant responses to biotic and abiotic stresses, including cold stress. However, they have not been well studied in the regulation of chlorophyll synthesis and cold tolerance. So it is meaningful to analyze the mechanism under cold stress in non-heading Chinese cabbage. Here, BcWRKY53, a transcriptional activator WRKY-III gene, was identified by a screen upstream of the key chlorophyll synthesis genes BcCHLH and BcGUN4. BcWRKY53 was localized in the cell nucleus and induced to a significant extent by cold treatment. Ectopic expression of BcWRKY53 in Arabidopsis not only increased the chlorophyll content under cold stress, but also improved the cold tolerance. After silencing of BcWRKY53, there was a decrease in chlorophyll content and an increase in cold sensitivity. BcWRKY53 could inhibit self-expression by binding W-boxes in its own promoter. In addition, histone deacetylase 9 (BcHDA9) interacted with BcWRKY53 to inhibit BcWRKY53-mediated transcriptional activation. When ectopically overexpressed, BcHDA9 negatively regulates chlorophyll content and cold tolerance under cold treatment. Taken together, this study demonstrated that the cold-inducible transcription factor BcWRKY53 positively regulates BcCHLH and BcGUN4 under the regulation of self-regulation and BcHDA9 interactions. In this way, BcWRKY53 is actively involved in chlorophyll synthesis and the establishment of cold tolerance, which providing practical theoretical support in molecular characterization of cold tolerance and variety selection of non-heading Chinese cabbage.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109398"},"PeriodicalIF":6.1,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The FLOWERING LOCUS T-like genes from patchouli (Pogostemon cablin) antagonistically regulate flowering time.","authors":"Manchun Wang, Jierong Huang, Yunping Zeng, ShiShi Song, Ying Zeng, Yanting Shen, Jianxin Wu, Puyue Ouyang, Honglei Jin, Hongbin Wang, Zhenyi Chang","doi":"10.1016/j.plaphy.2024.109394","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109394","url":null,"abstract":"<p><p>Flowering is crucial for the reproductive success of plants. Patchouli (Pogostemon cablin), a widely utilized medicinal and aromatic plant from the Lamiaceae family, exhibits rare flowering and fails to produce seeds, thereby posing a challenge for plant evolution and breeding improvement. However, the mechanism underlying flowering in patchouli has not been investigated. FLOWERING LOCUS T (FT) serves as a central integrator of flowering signals. Here, we identified 13 patchouli FT-like genes (PatFTs). In patchouli leaves, PatFT10-13 displayed continuous expression, with a decline noted at the flowering stage, while PatFT1-3 were activated exclusively at the flowering stage, and PatFT4-9 were hardly expressed. Overexpression of PatFT2 in Arabidopsis induced early flowering, while overexpression of PatFT10-13 resulted in delayed flowering. These results suggested that PatFT1-3, differing by one to two unique residues in the non-conserved region, might function as floral inducers, while PatFT10-13 likely act as floral repressors. Both PatFT2 and PatFT11 interacted with patchouli FD-like proteins. Transient expression of PatFT11 in protoplasts reduced the ability of PatFT2 to activate downstream flowering genes, suggesting a competitive antagonism between these proteins for shared interactors. Amino acid swapping analysis indicated that specific conserved residues was responsible for the functional switch in PatFTs. Furthermore, we revealed that the evolution of antagonistic FT-like modules might represent a common strategy for Lamiaceae plants to fine-tune flowering time. In summary, these findings provide new insights into the expansion and functional diversity of FT-like genes in patchouli.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109394"},"PeriodicalIF":6.1,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethylene negatively regulates cold tolerance through HbEIN3-HbICE2 regulatory module in Hevea brasiliensis.","authors":"Xue-Wei Zeng, Wei-Zeng Jiang, Jian-Long Zhang, Jia-Hui Ding, Yi-Min Qiu, Wei Wen, Huan Yang, Qian-Yu Zhang, Hong-Mei Yuan","doi":"10.1016/j.plaphy.2024.109397","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109397","url":null,"abstract":"<p><p>Cold stress can result in reduced growth rates, decreased latex production, and restricted areas for the Para rubber tree (Hevea brasiliensis). However, the molecular mechanisms governing the response of Hevea brasiliensis to cold stress remain elusive. Here, we found that ethylene plays a negative role in Hevea brasiliensis responses to cold stress. Treatment with the ethylene synthesis precursor 1-aminocyclopropane-1-carboxylic acid (ACC) decreased the cold tolerance of Hevea brasiliensis, while exogenous treatment with Ag⁺ (an ethylene signal inhibitor) had the opposite effect. Additionally, overexpressing HbEIN3 decreased cold stress tolerance in Arabidopsis and Taraxacum koksaghyz plants. Quantitative real-time PCR analysis indicated that HbEIN3-1 and HbEIN3-2 repress the expression of the cold-responsive genes HbCBF1-3 in Hevea brasiliensis. Moreover, HbEIN3-1 and HbEIN3-2 directly bind to the HbCBF1 promoter to suppress its transcription. Further investigation revealed that HbEIN3s interact with and dampen the transcriptional activity of HbICE2, a crucial transcription factor that positively regulates the cold signaling pathway, thereby attenuating the expression of HbICE2-targeted genes. Collectively, these findings indicate that HbEIN3s play a crucial role in ethylene-regulated cold tolerance through the repression of HbCBF1 expression and HbICE2 transcriptional activity.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109397"},"PeriodicalIF":6.1,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}