Plant Physiology and Biochemistry最新文献

Riboflavin improves postharvest cold tolerance in zucchini fruit inducing non-enzymatic antioxidant response and phenolic metabolism 核黄素能提高西葫芦果实的采后耐寒性，诱导非酶抗氧化反应和酚代谢

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-05 DOI: 10.1016/j.plaphy.2024.109270

{"title":"Riboflavin improves postharvest cold tolerance in zucchini fruit inducing non-enzymatic antioxidant response and phenolic metabolism","authors":"","doi":"10.1016/j.plaphy.2024.109270","DOIUrl":"10.1016/j.plaphy.2024.109270","url":null,"abstract":"<div><div>The storage of zucchini fruit at low temperatures during postharvest induces a physiological disorder called chilling injury that drastically reduces fruit quality and shelf life. The phytohormone abscisic acid (ABA) is involved in the acquisition of cold tolerance in zucchini fruit, being the riboflavin pathway one of the most differentially induced with ABA treatment. Thus, the aim of this work was to elucidate the involvement of riboflavin in quality maintenance of zucchini fruit during postharvest cold storage. After testing different concentrations of exogenous riboflavin, 0.5 mM showed the best results. Riboflavin treatment reduced H<sub>2</sub>O<sub>2</sub> content, but the enzymatic antioxidant defense did not change significantly. This response is due to a rise of non-enzymatic antioxidant defense, by accumulating metabolites like ascorbate and carotenoids, as well as inducing phenolic metabolism. The application of this vitamin enhanced the phenolic and flavonoid content in fruit, concomitant with an induction of PAL and C4H activities and an inhibition of PPO activity. This enhancement of the phenylpropanoid pathway resulted in high vanillic acid and quercetin levels at first day of cold storage. The induction of numerous antioxidant compounds and abscisic acid by riboflavin treatment at short-term postharvest period could be responsible for the lack of chilling injuries in zucchini fruit. Therefore, riboflavin could be successfully implemented in the food industry as an alternative to physical or chemical treatments, due to it is an innocuous additive with good water solubility and low cost, as it prolongs the shelf-life of zucchini fruit and increases its nutraceutical properties.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infection of tomato plants by tomato yellow leaf curl virus (TYLCV) potentiates the ethylene and salicylic acid pathways to fend off root-knot nematode (Meloidogyne incognita) parasitism 番茄黄叶卷曲病毒（TYLCV）感染番茄植株后会增强乙烯和水杨酸途径抵御根结线虫（Meloidogyne incognita）寄生的能力

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-04 DOI: 10.1016/j.plaphy.2024.109271

{"title":"Infection of tomato plants by tomato yellow leaf curl virus (TYLCV) potentiates the ethylene and salicylic acid pathways to fend off root-knot nematode (Meloidogyne incognita) parasitism","authors":"","doi":"10.1016/j.plaphy.2024.109271","DOIUrl":"10.1016/j.plaphy.2024.109271","url":null,"abstract":"<div><div>In nature, it is common for plants to be infected by multiple pathogens simultaneously, and deciphering the underlying mechanisms of such interactions has remained elusive. The occurrence of root-knot nematode (RKN), <em>Meloidogyne incognita</em>, and tomato yellow leaf curl virus (TYLCV; <em>Begomovirus coheni</em>) has been reported in most tomato cultivation areas. We investigated the interaction between RKN and TYLCV in tomato plants at phenotypic, biochemical, and gene expression levels. Several treatments were considered including mock inoculation, inoculation with TYLCV or RKN alone, simultaneous inoculation with both TYLCV and RKN, and sequential inoculations with a five-day interval. Among them, simultaneous inoculation showed the highest impact on RKN suppression compared to mock-inoculated plants. Biochemical assays in the time-point experiments demonstrated that the pick of defense capacity of plants occurs at 48- and 72-h post-inoculation. Gene expression analyses utilizing marker genes from main hormonal pathways involved in plant defense, including salicylic acid (SA), jasmonic acid (JA), and ethylene (ET), indicated that ET and SA are highly involved in the potentiation of TYLCV-induced defense against RKN. To validate the action of SA and ET in the induction of defense against RKN by TYLCV, transgenic lines deficient in SA (<em>NahG</em>) and ET (<em>ACD</em>) accumulation were co-inoculated with TYLCV and RKN. Both transgenic lines failed to express TYLCV-induced defense against RKN. These findings demonstrate an antagonistic effect of TYLCV against RKN in tomato plants, mediated by SA and ET signaling pathways.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of water deficit on two cultivars of Hibiscus mutabilis: A comprehensive study on morphological, physiological, and metabolic responses 缺水对变异木槿两个栽培品种的影响：形态、生理和代谢反应的综合研究。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-04 DOI: 10.1016/j.plaphy.2024.109269

{"title":"Effects of water deficit on two cultivars of Hibiscus mutabilis: A comprehensive study on morphological, physiological, and metabolic responses","authors":"","doi":"10.1016/j.plaphy.2024.109269","DOIUrl":"10.1016/j.plaphy.2024.109269","url":null,"abstract":"<div><div><em>Hibiscus mutabilis</em>, commonly known as the cotton rose, is a widely cultivated ornamental and has been acclaimed as the representative flower of the 2024 World Horticultural Exposition. The growth and ornamental characteristics of <em>Hibiscus mutabilis</em> can be affected by drought stress. Therefore, we investigated the physiological and metabolic responses of drought-sensitive <em>Hibiscus mutabilis</em> JRX-1 and drought-tolerant <em>Hibiscus mutabilis</em> CDS-4 under drought stress. The results of the physiological analyses revealed that, compared to JRX-1,CDS-4 maintained good growth and greater water use efficiency through stronger antioxidant defences, osmoregulatory capacity and stomatal regulation. A total of 3277 metabolites were identified in positive and negative ion modes, of which 663 metabolites presented changes in expression under drought conditions, including 306 upregulated metabolites and 357 downregulated metabolites. Secondary metabolites, such as flavonoids and diterpenoids, are crucial in the plant response to drought stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the differentially aboundant metabolites were significantly enriched in the pathways valine, leucine and isoleucine degradation; linoleic acid metabolism; one carbon pool by folate; and folate biosynthesis. The results of this study will not only help to elucidate and apply the physiological and metabolic regulatory strategies of <em>Hibiscus mutabilis</em> to improve its adaptation to water deficit conditions, but will also provide valuable guidance to breeders and molecular biologists in the screening and use of drought resistant genes in ornamental plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling contribution and fate of nitrogen with 15N techniques affected by microbial co-inoculation on field-grown maize: A novel approach to optimize N-fertilizer use efficiency 利用 15N 技术揭示受田间种植玉米上微生物共同接种影响的氮的贡献和归宿：优化氮肥利用效率的新方法

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-02 DOI: 10.1016/j.plaphy.2024.109261

{"title":"Unveiling contribution and fate of nitrogen with 15N techniques affected by microbial co-inoculation on field-grown maize: A novel approach to optimize N-fertilizer use efficiency","authors":"","doi":"10.1016/j.plaphy.2024.109261","DOIUrl":"10.1016/j.plaphy.2024.109261","url":null,"abstract":"<div><div>The objectives of this research were to: i) develop a mechanistic understanding of the synergy between microbial co-inoculation, nitrogen (N) fertilizer, and maize plants on biological <sup>15</sup>N fixation, and <sup>15</sup>N-recovery from applied fertilizers; and ii) explore the mechanist effects of microbial co-inoculation on N fractionations and derivation (fertilizer, atmosphere and soil), physiological responses on water use and carboxylation efficiencies and growth by using two different isotopic techniques under field conditions. Treatments included four seed inoculations (Control, <em>B. subtilis</em>, <em>A. brasilense</em>, and the combination of <em>B. subtilis</em> and <em>A. brasilense</em>), along with five levels of N application (0–240 kg N ha<sup>−1</sup>). Overall, the results indicate that maize co-inoculation with the above-mentioned bacteria enhanced photosynthetic efficiency leading to improved carboxylation efficiency and instantaneous water use efficiency in maize plants, likely due to an increase in net photosynthetic rate. This effect was more evident under low N availability. The findings also suggest that co-inoculation enhanced the ability of maize plants to absorb CO<sub>2</sub>, adjust to different soil N levels, and carry out photosynthesis, which resulted in higher carbon fixation and better maize growth. The N obtained from the atmosphere resulting from inoculation ranged from 25 to 50 kg N ha<sup>−1</sup>. Nonetheless, N application rates exceeding 186 kg N ha<sup>−1</sup> substantially diminished the ability of these bacteria to fix N<sub>2</sub>. The combination of inoculation with the application of 120–180 kg N ha<sup>−1</sup> led to a synergistic effect resulting in the greatest N-use efficiency, -recovery and grain yield.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining transcriptome and metabolome analyses to reveal the response of maize roots to Pb stress 结合转录组和代谢组分析揭示玉米根系对铅胁迫的响应

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-02 DOI: 10.1016/j.plaphy.2024.109265

{"title":"Combining transcriptome and metabolome analyses to reveal the response of maize roots to Pb stress","authors":"","doi":"10.1016/j.plaphy.2024.109265","DOIUrl":"10.1016/j.plaphy.2024.109265","url":null,"abstract":"<div><div>As a major food crop, maize (<em>Zea mays</em> L.) is facing a serious threat of lead (Pb) pollution. Research into its Pb tolerance is crucial for ensuring food security and human health, however, the molecular mechanism underlying the response to Pb remains incompletely understood. Here, we investigated the transcriptomic and metabolome of two maize lines (BY001, a Pb-resistant line; BY006, a Pb-sensitive line) under different concentrations of Pb stress (0, 500, 1000, 2000 and 3000 mg/L). The results showed that BY001 performed well, whereas the BY006 exhibited minimal development of lateral roots upon exposure to high concentration of Pb. The antioxidant enzyme activity of BY001 remained relatively stable, while that of BY006 declined significantly. Transcriptomic analysis revealed that under high concentration of Pb stress, BY001 produced 5057 differentially expressed genes, whereas BY006 produced 3374. Functional annotation showed that these genes were primarily involved in carbohydrate metabolism, root growth, and plant resistance to external Pb stress. Further untargeted metabolomics indicated that Pb stress triggered distinct alterations in the levels of 47 diverse metabolite types across 13 distinct classes, particularly amino acids, carbohydrates, and organic acids. A conjoint omics analysis suggested that the pathways of starch and sucrose metabolism, as well as cutin, suberin, and wax biosynthesis in BY001, play a key role in the Pb resistance. These findings elucidate the biological mechanisms employed by maize to counter the effects of Pb stress, and provide a basis for breeding of maize cultivars with low Pb accumulation or tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of green and UVA spectra, and pre-harvest treatments on biomass and metabolite yields of indoor cultivated stevia rebaudiana 绿光谱和 UVA 光谱以及收获前处理对室内栽培甜叶菊生物量和代谢物产量的影响

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-02 DOI: 10.1016/j.plaphy.2024.109252

{"title":"Effect of green and UVA spectra, and pre-harvest treatments on biomass and metabolite yields of indoor cultivated stevia rebaudiana","authors":"","doi":"10.1016/j.plaphy.2024.109252","DOIUrl":"10.1016/j.plaphy.2024.109252","url":null,"abstract":"<div><div><em>Stevia rebaudiana</em> is a high-value crop due to the strong commercial demand for its metabolites (steviol glycosides, SG) as an organic low-caloric sweetener with up to 300 times the sweetness of conventional sugar. Two experiments were conducted in this study. In the first experiment, treatments with varying green (GR1 & GR2), UVA (UV1 & UV2) and treatments that had both (UVGR1, UVGR2) were used. In the second experiment, separate set of plants were grown under base red-blue (RB) and natural sunlight before being transferred to GR2, UV2, UVGR2, and monochromatic light treatments of blue, green and UVA, for 3 and 10 days before harvest. RB and sunlight were used as the control for artificial and natural light respectively. Plants grown under the UVGR1 had the highest dry leaf biomass accumulation of 4.75 g plant<sup>−1</sup> (P < 0.05), 458% and 660% higher than the RB (0.98 g plant<sup>−1</sup>) and natural sunlight (0.72 g plant<sup>−1</sup>) controls. UVA had the highest metabolite (Stevioside + Rebaudioside A) concentration of 27% (P < 0.05) compared to the RB and sunlight controls at 17.24% and 15% respectively. The 10 day pre-harvest treatment with blue supplemented light yielded a dry biomass of 1.87 g plant<sup>-</sup>1, a 190% increase compared to the RB control. However, the 3 day pre-harvest treatment had higher metabolite yields improvements compared to 10 day treatments with the highest yield obtained of 21.10% in 3-day pre-harvest irradiation that had supplemental UVA and blue light. UVGR1 was the most productive lighting strategy, resulting in the highest overall metabolite yield per plant.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "From non-coding RNAs to histone modification: The epigenetic mechanisms in tomato fruit ripening and quality regulation" [Plant Physiol. Biochem. 215 (2024), 109070]. 从非编码 RNA 到组蛋白修饰：番茄果实成熟和品质调控的表观遗传机制" [Plant Physiol.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-01 Epub Date: 2024-09-14 DOI: 10.1016/j.plaphy.2024.109102

Shengbo Chen, Jiazhen Han, Shu Wu, Shangjing Guo, Yufei Tang, Yujing Zheng, Lei Hu, Xingxing Zhang, Peng Zhang, Haifeng Zhang, Guodong Ren, Shuai Gao

引用次数: 0

Nutritional stress in plants: Understanding sensing and resilience 植物的营养压力：了解感应和恢复能力

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-01 DOI: 10.1016/j.plaphy.2024.109207

引用次数: 0

Editorial to special issue on Photosynthetic organisms for sustainable development. 为 "光合生物促进可持续发展 "特刊撰写社论。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-01 DOI: 10.1016/j.plaphy.2024.109263

Giorgio Perin, Alexandra Dubini, Francesco Milano, Joanna Kargul, Maya Dimova Lambreva

引用次数: 0

Real-time monitoring of stromal NADPH levels in Arabidopsis using a metagenome-derived NADPH-binding fluorescent protein 利用源于元基因组的 NADPH 结合荧光蛋白实时监测拟南芥基质 NADPH 水平

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-01 DOI: 10.1016/j.plaphy.2024.109260

{"title":"Real-time monitoring of stromal NADPH levels in Arabidopsis using a metagenome-derived NADPH-binding fluorescent protein","authors":"","doi":"10.1016/j.plaphy.2024.109260","DOIUrl":"10.1016/j.plaphy.2024.109260","url":null,"abstract":"<div><div>The light irradiation to the plant chloroplasts drives NADPH and ATP synthesis in the stroma via the electron transport chains within the thylakoid membranes. Conventional methods for assessing photosynthetic light reactions are often invasive or require specific conditions. While detection markers do not significantly affect plant growth itself, developing a method for the real-time and non-invasive detection of NADPH is a highly impactful and important research area in plant physiology and biochemistry. This study introduces a genetically encoded NADPH-binding blue fluorescent protein (mBFP) targeted to the chloroplast stroma or thylakoid membrane in <em>Arabidopsis thaliana</em> and <em>Nicotiana benthamiana</em>. Using two-photon microscopy, we monitored real-time stromal NADPH levels in transgenic leaves of Arabidopsis in response to light exposure. A mutant mBFP construct targeted to the thylakoid membrane allowed us to detect the stromal NADPH levels in real time under different light conditions. This <em>in planta</em> biosensor provides a non-invasive tool for studying photosynthetic responses to light more quantitatively and holds potential for optimizing light conditions in controlled-environment agriculture, such as indoor vertical farms, to improve crop productivity.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0