Plant Physiology and Biochemistry最新文献

{"title":"Corrigendum to “PbARP1 enhances salt tolerance of ‘Duli’ pear (Pyrus betulifolia Bunge) through abscisic acid signalling pathway” [Plant Physiology and Biochemistry 227 (2025) 110075]","authors":"Rui Liu , Ziyi Li , Huaixuan Wang , Haixia Zhang , Jianfeng Xu , Yuxing Zhang","doi":"10.1016/j.plaphy.2025.110585","DOIUrl":"10.1016/j.plaphy.2025.110585","url":null,"abstract":"","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110585"},"PeriodicalIF":5.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethylene promotes lateral root formation following waterlogging in an epiphytic orchid, Cymbidium tracyanum","authors":"Tian-Yang Gao ,&nbsp;Ning-Yu Liu ,&nbsp;Mengling Tu ,&nbsp;Hai-Cui Luo ,&nbsp;Shi-Bao Zhang","doi":"10.1016/j.plaphy.2025.110575","DOIUrl":"10.1016/j.plaphy.2025.110575","url":null,"abstract":"<div><div>Waterlogging is a major abiotic stress that severely impairs plant growth, development and productivity. However, the mechanisms governing recovery from waterlogging stress in plants, particularly in epiphytes, remain poorly understood. Focused on lateral root (LR) formation, this study explored the physiological changes and key gene expression dynamics underlying root development in <em>Cymbidium tracyanum</em> during waterlogging and recovery to elucidate recovery mechanisms in epiphytic orchids. Significant morphological changes in <em>C. tracyanum</em> occurred during the recovery period rather than the waterlogging period. Transcriptome analysis revealed extensive transcriptional reprogramming during waterlogging and recovery, particularly in genes related to phytohormone biosynthesis and signaling pathways. Phytohormone profiling showed rapid ethylene accumulation during waterlogging, with earlier activation of ethylene biosynthesis and signaling genes compared to other hormones. Exogenous application of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) under non-stressed condition promoted LR formation and triggered jasmonate accumulation, mimicking the natural recovery response. Weighted gene co-expression network analysis, along with gene function annotation and expression analyses, identified <em>CtNAC68</em> and <em>CtWRKY65</em> as key genes responsive to ethylene and jasmonate signaling pathways. Collectively, these results demonstrated that lateral root formation, a process regulated by ethylene and jasmonate signaling, was a strategy employed by <em>C. tracyanum</em> to adapt to waterlogging stress. The findings provide the first robust evidence for the role of lateral root formation in waterlogging adaptation of epiphytic orchids, offering novel insights into their ecological adaptation and evolution.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110575"},"PeriodicalIF":5.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-targeted mutagenesis of the PpTCP4 gene increased tiller number in Poa pratensis","authors":"Cheng Li ,&nbsp;Chen Meng ,&nbsp;Xinzhi Wang ,&nbsp;Chao Yuan ,&nbsp;Qian Chen ,&nbsp;Ya Yang ,&nbsp;Zhengshe Zhang ,&nbsp;Yuanwen Duan ,&nbsp;Quanmin Dong ,&nbsp;Xudong Sun","doi":"10.1016/j.plaphy.2025.110588","DOIUrl":"10.1016/j.plaphy.2025.110588","url":null,"abstract":"<div><div><em>Poa pratensis</em>, commonly known as Kentucky bluegrass, is a widely used cool-season grass species for turf in lawns and recreational areas worldwide. However, the lack of genome editing platforms has impeded research into enhancing forage crops suitable for land unsuitable for other crops. In this study, we successfully established an <em>Agrobacterium</em>-mediated genetic transformation system for <em>P</em>. <em>pratensis</em>. Furthermore, we successfully produced <em>PpTCP4</em>-edited plants using the CRISPR/Cas9 system. This research represents a significant advancement in the genetic transformation and gene editing of <em>P. pratensis</em>, providing a foundational technology for future investigations into gene function and breeding in <em>P. pratensis</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110588"},"PeriodicalIF":5.7,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rice specific novel miRNA, Osa-miR39 targets 9-cis-epoxycarotenoid dioxygenases (NCEDs) to regulate fluoride stress response","authors":"Tamarapalli Sravya Sruti,&nbsp;Sasmita Mohanty,&nbsp;Raj Kumar Joshi","doi":"10.1016/j.plaphy.2025.110582","DOIUrl":"10.1016/j.plaphy.2025.110582","url":null,"abstract":"<div><div>Fluoride contamination in rice poses a serious risk to human health. MicroRNAs (miRNAs) regulate gene expression under stress conditions, yet their role in fluoride stress remains unclear. Comparative profiling of fluoride-sensitive (IR-64) and tolerant (Gobindobhog, GB) cultivars identified 436 fluoride-responsive miRNAs, of which 39 (30 conserved and 9 novel) were differentially expressed. Target prediction and expression analyses revealed regulation of stress-associated transcription factors, with inverse correlations between several miRNAs and their targets. A novel miRNA, Osa-miR39, was strongly induced in GB but suppressed in IR-64 under fluoride stress. The expression levels of its three abscisic acid (ABA)-responsive target genes encoding 9-cis-epoxycarotenoid dioxygenases (NCED3 &amp; NCED5) and aldehyde oxidase (AO) were suppressed in GB, suggesting a potential role in fluoride tolerance. The regulatory interaction between Osa-miR39 and its targets was further validated through transient co-expression assays in <em>Nicotiana benthamiana</em>. Moreover, the overexpression of Osa-miR39 in the sensitive rice cultivar IR-64 conferred enhanced fluoride tolerance concomitant with repression of <em>OsNCED</em> genes. Collectively, these findings identify Osa-miR39 as a key regulator of fluoride stress tolerance in rice.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110582"},"PeriodicalIF":5.7,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145266482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ca2+ signaling is required for high ambient temperature perception in Arabidopsis thaliana","authors":"Mengyun Wang ,&nbsp;Yuqing Wang ,&nbsp;Jia Wang ,&nbsp;Zhen-Ming Pei ,&nbsp;Yibo Teng","doi":"10.1016/j.plaphy.2025.110586","DOIUrl":"10.1016/j.plaphy.2025.110586","url":null,"abstract":"<div><div>Temperature is a key environmental factor affecting plant growth and development. With the increase in global warming, it is important to understand the signal transduction pathways through which plants perceive and respond to elevated ambient temperature. Calcium (Ca²⁺) is a second messenger in various environmental stresses in plants. However, the function of Ca²⁺ in sensing elevated ambient temperature has not yet been well addressed in plants. In this study, using the Ca²⁺ sensitive aequorin reporter or GFP-based yellow cameleon 3.6 reporter in plants, we show that high temperature treatments transiently increase cytoplasmic Ca²⁺ concentration in <em>Arabidopsis thaliana</em>. We also found that Ca²⁺ is required for warm temperature-induced hypocotyl growth. In addition, our RNA-seq data show that transcriptional reprogramming in response to warm temperature is partially dependent on Ca²⁺. We further identified Ca²⁺ signaling-related components involved in the decoding of the response to elevated ambient temperature to support our hypothesis. Taken together, our results suggest that plants possess a Ca²⁺ signaling pathway to sense thermal changes.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110586"},"PeriodicalIF":5.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145266486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of four terpene synthase genes involved in nerolidol and linalool biosynthesis in Santalum album leaves induced by methyl jasmonate","authors":"Yuqing Wang ,&nbsp;Chuting Wu ,&nbsp;Yuping Xiong ,&nbsp;Zhan Bian ,&nbsp;Yunfei Yuan ,&nbsp;Yongxia Jia ,&nbsp;Jaime A. Teixeira da Silva ,&nbsp;Guohua Ma ,&nbsp;Xinhua Zhang","doi":"10.1016/j.plaphy.2025.110571","DOIUrl":"10.1016/j.plaphy.2025.110571","url":null,"abstract":"<div><div><em>Santalum album</em> is highly valued for its fragrant essential oil from heartwood. Volatile terpenoids involved in aroma formation in plants can be emitted in response to a variety of environmental stresses. However, the regulatory mechanisms underpinning the response of <em>S. album</em> to external stresses are not yet known. In this study, the regulatory mechanism of <em>S. album</em> leaves was investigated after the application of methyl jasmonate (MeJA), a stressor. Eight classes of volatile organic compounds were identified in <em>S. album</em> leaves, including terpenes, aldehydes, alcohols, ketones, esters, benzenoids, alkanes and heterocyclic compounds. In total, 15 terpenoids, such as ocimene, linalool, nerolidol and α-farnesene, were considerably induced 6 h after MeJA treatment, amounting to 38.01 % of all volatiles. Notably, transcript levels of the genes in the MVA pathway were enhanced by 2- to 8-fold after 6 h of MeJA treatment compared to the control. Exogenously applied MeJA resulted in the significant upregulation of ten terpene synthase (SaTPS) genes. <em>In vitro</em> enzyme activity assays confirmed that four SaTPS recombinant proteins converted substrates into (<em>E</em>)-nerolidol and linalool. Overexpression of the four <em>SaTPS</em> genes produced (<em>E</em>)-nerolidol, (<em>Z</em>)-nerolidol and linalool in sandalwood callus. These results suggest that the four <em>SaTPS</em> genes have the ability to synthesize nerolidol and linalool in <em>S. album</em> leaves in response to MeJA treatment. This study provides insight into the regulatory mechanism of the biosynthesis of terpenoids in <em>S. album</em> in response to an environmental stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110571"},"PeriodicalIF":5.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cycloartenol-derived triterpenoid pathway genes alter the root metabolome and microbiome in tomato","authors":"Alessandra Guerrieri ,&nbsp;Davar Abedini ,&nbsp;Fred White ,&nbsp;Jurre Bleeker ,&nbsp;Gertjan Kramer ,&nbsp;Lemeng Dong","doi":"10.1016/j.plaphy.2025.110584","DOIUrl":"10.1016/j.plaphy.2025.110584","url":null,"abstract":"<div><div>Plant triterpenoids derived from cycloartenol are central to sterol homeostasis and specialized metabolite production, yet their roles in shaping rhizosphere interactions remain poorly understood. Here, we investigated the function of key cycloartenol-derived triterpenoid biosynthetic genes in tomato (Solanum lycopersicum) by transiently silencing <em>CYCLOARTENOL SYNTHASE 1</em> (<em>SlCAS1</em>), <em>STEROL METHYLTRANSFERASE 1</em> (<em>SlSMT1</em>), <em>STEROL SIDE CHAIN REDUCTASE 2</em> (<em>SlSSR2</em>), <em>and PHYTOENE DESATURASE</em> (<em>SlPDS</em>). <em>SlCAS1</em> suppression caused severe growth inhibition, confirming the essential role of cycloartenol for plant development. Silencing of <em>SlSMT1</em> and <em>SlSSR2</em> altered root sterol composition, with <em>SlSMT1</em> reducing β-sitosterol and stigmasterol, and <em>SlSSR2</em> causing decreases in cholesterol as well as significant reductions in steroidal glycoalkaloids (SGAs) and steroidal saponins (SAs). By contrast, <em>SlPDS</em> silencing unexpectedly led to elevated sterol levels and broad metabolome shifts. Untargeted metabolomics revealed gene-specific alterations in root and exudate profiles, while molecular networking highlighted the rapid loss of SGAs in exudates, suggesting microbial degradation. Integration of metabolomic and 16S rRNA sequencing data showed that changes in sterols, SGAs, and saponins were associated with distinct bacterial families, including Comamonadaceae and Sphingomonadaceae. Together, these findings demonstrate that cycloartenol-derived triterpenoid pathway genes strongly influence root metabolite composition and shape the assembly of tomato root-associated microbial communities.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110584"},"PeriodicalIF":5.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of multi-sensing technologies for high-throughput morphological, physiological, and biochemical phenotyping of drought-stressed watermelon plants","authors":"Mohammad Akbar Faqeerzada ,&nbsp;Eunsoo Park ,&nbsp;Jinsu Lim ,&nbsp;Kihyun Kim ,&nbsp;Ramaraj Sathasivam ,&nbsp;Sang Un Park ,&nbsp;Hangi Kim ,&nbsp;Byoung-Kwan Cho","doi":"10.1016/j.plaphy.2025.110577","DOIUrl":"10.1016/j.plaphy.2025.110577","url":null,"abstract":"<div><div>High-throughput plant phenotyping (HTPP) technologies are rapidly transforming plant science by enabling real-time, non-invasive, and large-scale monitoring of complex morphological, physiological, and biochemical traits. However, existing platforms often lack integration across sensing modalities and analytical depth necessary for early and comprehensive phenotypic trait analysis. In this study, we developed a fully automated, multimodal HTPP system combining RGB, shortwave infrared (SWIR) hyperspectral, multispectral fluorescence imaging (MSFI), and thermal imaging to characterize drought-stressed watermelon (<em>Citrullus lanatus</em>) plants. RGB imaging facilitated detailed morphological analysis by extracting color-based traits, quantifying plant height and canopy area, and accurately distinguishing growth stages. SWIR hyperspectral imaging (HSI) enabled non-invasive biochemical assessment by detecting drought-responsive compounds, such as flavonoids, phenolics, and antioxidant activities, while also supporting the classification of stress severity. This spectral profiling revealed key biochemical alterations triggered by water deficit. MSFI liquid crystal tunable filter (LCTF-based) measured chlorophyll <em>a</em> (Chl-a), chlorophyll <em>b</em> (Chl-b), and total chlorophyll (t-Chl) levels, providing critical insights into photosynthetic performance under drought stress. Thermal imaging further enhanced drought assessment by capturing canopy temperature variations, which were used to derive thermal indices for indirect estimation of soil volumetric water content (SVWC). By integrating complementary imaging modalities, the proposed system captured comprehensive phenotypic responses with high predictive accuracy for early detection of drought stress and assessment of plant health. Advanced machine learning (ML) and deep learning (DL) models further enhanced trait extraction and classification, enabling robust analysis of complex, high-dimensional data. This automated, multimodal platform offers scalable, non-invasive crop monitoring, providing precise insights to support drought resilience and precision agriculture.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110577"},"PeriodicalIF":5.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Shadow government\": how transcription factors regulate plant cell wall formation","authors":"Natalia Mokshina,&nbsp;Natalya Syrchina","doi":"10.1016/j.plaphy.2025.110589","DOIUrl":"10.1016/j.plaphy.2025.110589","url":null,"abstract":"<div><div>The plant cell wall is a dynamic, structurally complex supramolecular compartment essential for the development, defense, and adaptation of plants to their environment. While the biosynthesis and transcriptional regulation of secondary cell walls (SCWs) have been extensively studied and are relatively well characterized, the hierarchical transcriptional regulatory networks (TRNs) orchestrating the formation of primary and tertiary cell walls (PCWs and TCWs) remain less well understood. Although all plant cells possess PCWs, the regulation of their biosynthesis remains enigmatic. Even less is known about TCWs, which are exclusive to fibers. This review offers a comprehensive summary of the current understanding of the transcriptional regulation of plant cell wall biosynthesis, highlighting recent progress as well as ongoing knowledge gaps. We examine the inherent challenges in studying PCWs, given their indispensable role in cell viability, which complicates experimental dissection. Meanwhile TCW formation, inherent to specialized mechanical tissue such as sclerenchyma, is controlled by a distinct tissue-specific regulatory program, elements of which remain unidentified and appear fundamentally different from those of the SCW TRN, despite TCWs being considered a layer within SCWs. Though these programs remain elusive, they exhibit all the characteristics of a well-organized \"shadow government\": influential, precisely coordinated, and remarkably difficult to detect. By synthesizing insights across diverse plant systems and incorporating the latest genomic and transcriptomic approaches, this review outlines the current state of plant cell wall regulation research and identifies promising directions for future investigation.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110589"},"PeriodicalIF":5.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145266468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of TaNAC6-3B: A key regulator of drought tolerance in wheat (Triticum aestivum L.)","authors":"Nan Chen ,&nbsp;Xiang Li ,&nbsp;Yong-jia Feng ,&nbsp;De-jun Han ,&nbsp;Wei-jun Zheng ,&nbsp;Zhen-sheng Kang","doi":"10.1016/j.plaphy.2025.110578","DOIUrl":"10.1016/j.plaphy.2025.110578","url":null,"abstract":"<div><div>Drought stress is a major abiotic constraint limiting wheat (<em>Triticum aestivum</em> L.) productivity. Previous studies have shown that reducing irrigation water use by approximately 40 % can cause a 20.6 % decrease in wheat yield. Therefore, improving drought resistance is a priority in wheat breeding programs. For genetic improvement of drought tolerance, systematic investigation of drought-responsive molecular mechanisms is crucial. In this study, comparative transcriptome analysis was conducted on leaf and root tissues of the drought-tolerant wheat cultivar ChangWu134 under well-watered and drought-stressed conditions. Further systematic analysis identified a key drought tolerance gene <em>TaNAC6-3B.</em> Functional characterization of the candidate NAC (NAM, ATAF1/2, and CUC2) family transcription factor <em>TaNAC6-3B</em> revealed its nuclear localization. Transgenic overexpression lines had significantly enhanced drought tolerance, and transcriptome profiling revealed up-regulation of NCED and ABA responsive genes and drought-responsive genes. Mechanism studies have revealed that <em>TaNAC6-3B</em> activates the expression of the LEA (Late embryogenesis abundant) protein gene <em>TaLEA1-2B</em> via direct binding to its promoter. The results of this study provide clues for analysis of the genetic basis of drought tolerance in ChangWu134, and also provide candidate genetic resources for breeding for drought tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110578"},"PeriodicalIF":5.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}