Plant Physiology and Biochemistry最新文献_第2页

Maize ZmWRKY71 gene positively regulates drought tolerance through reactive oxygen species homeostasis.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-09 DOI: 10.1016/j.plaphy.2024.109399

Zhongxian Ma, Yue Jia, Yongwei Min, Xiu Fang, Haidong Yan, Qing Ma, Ronghao Cai

{"title":"Maize ZmWRKY71 gene positively regulates drought tolerance through reactive oxygen species homeostasis.","authors":"Zhongxian Ma, Yue Jia, Yongwei Min, Xiu Fang, Haidong Yan, Qing Ma, Ronghao Cai","doi":"10.1016/j.plaphy.2024.109399","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109399","url":null,"abstract":"Drought stress severely affects plant growth and yield. The plant-specific WRKY transcription factors play an important role in regulating the plant response to abiotic stresses. In this study, we identified a group I WRKY gene from maize, designated ZmWRKY71. Real-time quantitative reverse transcription-PCR analysis revealed that ZmWRKY71 was predominantly expressed in the roots and was induced by drought. ZmWRKY71 was localized in the nucleus and showed transcriptional activity in yeast. Heterologous overexpression of ZmWRKY71 improved drought tolerance in yeast and Arabidopsis. Compared with the wild type, the overexpression lines showed a higher survival rate under drought stress with reduced malondialdehyde content and elevated antioxidant enzyme activities. In contrast, mutation of ZmWRKY71 in maize leads to increased sensitivity to drought stress, reduced survival, elevated concentrations of reactive oxygen species, and increased malondialdehyde content. RNA-sequencing analysis revealed that the expression patterns of genes associated with translation, membrane, and oxidoreductase activity pathways were altered under drought stress. Yeast one-hybrid, dual-luciferase, and electrophoretic mobility shift assays confirmed that ZmWRKY71 was capable of directly binding to the W-box element in the promoter region of ZmPOD42 (Zm00001eb330550). Taken together, the results show that ZmWRKY71 positively regulates maize drought tolerance. This research enriches the drought tolerance gene pool for maize and provides a theoretical basis for maize drought tolerance breeding.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109399"},"PeriodicalIF":6.1,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of the auxin-response factor family in moso bamboo reveals that PeARF41 negatively regulates second cell wall formation.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-07 DOI: 10.1016/j.plaphy.2024.109395

Kebin Yang, Huiling Zhang, Letong Sun, Yue Zhang, Zhimin Gao, Xinzhang Song

{"title":"Identification and characterization of the auxin-response factor family in moso bamboo reveals that PeARF41 negatively regulates second cell wall formation.","authors":"Kebin Yang, Huiling Zhang, Letong Sun, Yue Zhang, Zhimin Gao, Xinzhang Song","doi":"10.1016/j.plaphy.2024.109395","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109395","url":null,"abstract":"Auxin response factors (ARFs) are key transcriptional factors mediating the transcriptional of auxin-related genes that play crucial roles in a range of plant metabolic activities. The characteristics of 47 PeARFs, identified in moso bamboo and divided into three classes, were evaluated. Structural feature analysis showed that intron numbers ranged from 3 to 14, while Motif 1, 2, 7 and 10 were highly conserved, altogether forming DNA-binding and ARF domains. Analysis of RNA-seq from different tissues revealed that PeARFs showed tissue-specificity. Additionally, abundant hormone-response and stress-related elements were enriched in promoters of PeARFs, supporting the hypothesis that the expression of PeARFs was significantly activated or inhibited by ABA and cold treatments. Further, PeARF41 overexpression inhibited SCW formation by reducing hemicellulose, cellulose and lignin contents. Moreover, a co-expression network, containing 28 genes with PeARF41 at its core was predicted, and the results of yeast one hybridization (Y1H), electrophoretic mobility shift assay (EMSA) and dual-luciferase (Dul-LUC) assays showed that PeARF41 bound the PeSME1 promoter to inhibit its expression. We conclude that a 'PeARF41-PeSME1' regulatory cascade mediates SCW formation. Our findings provided a solid theoretical foundation for further research on the role of PeARFs.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109395"},"PeriodicalIF":6.1,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary analysis of CBFs/DREB1s in temperate and tropical woody bamboos and functional study of PeDREB1A3 under cold and drought stress.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-06 DOI: 10.1016/j.plaphy.2024.109392

Kaiting Wang, Shijie Huang, Xiangyu Li, Jiahao Ma, Ying Li, Simeng Kong, Xinchun Lin

{"title":"Evolutionary analysis of CBFs/DREB1s in temperate and tropical woody bamboos and functional study of PeDREB1A3 under cold and drought stress.","authors":"Kaiting Wang, Shijie Huang, Xiangyu Li, Jiahao Ma, Ying Li, Simeng Kong, Xinchun Lin","doi":"10.1016/j.plaphy.2024.109392","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109392","url":null,"abstract":"Bamboo forests are vulnerable to extreme cold, as well as drought caused by declining rainfall or persistent hot, under global climate change. The C-repeat binding factors/dehydration-responsive element binding protein 1s (CBFs/DREB1s) are vital to acquiring tolerance to deal with the changing climate in plants. Herein, we investigated the evolution of CBFs/DREB1s in four temperate or tropical woody bamboos. In Phyllostachys edulis, Hsuehochloa calcarea, Dendrocalamus latiflorus, and Dendrocalamus brandisii, a total of 16, 12, 24, and 22 putative DREB1s were identified and were categorized into nine subclades, from DREB1A to DREB1I. DREB1s members increased with bamboo polyploidization, coinciding with the presence of at least two collinear DREB1s orthologs in different bamboos. It indicates the importance of polyploidization in driving the expansion of DREB1s. Except for the DREB1F, DREB1s of the other subclades showed direct collinearity with their orthologs in Poaceae. Tandemly linked loci of DREB1A, DREB1H, and DREB1B were of concern due to their conserved and inherited relationship in bamboo, and a recent duplication of DREB1A occurred during bamboo speciation. In P. edulis, PeDREB1A3/PeDREB1H1/PeDREB1B3 locus showed sensitivity to cold stimulation, especially for PeDREB1A3 rapidly induced after 0.5-h cold stimulation. PeDREB1A3 was proved as a nuclear-located transcription activator recognizing DRE cis-element. Moreover, overexpression of PeDREB1A3 improved both cold and drought tolerance of Arabidopsis thaliana. It suggested that the neoteric duplication of DREB1As might contribute to bamboo adaptability. DREB1A represents the potential locus for improving agronomic traits in the future. This research provides valuable information for excavating potential genes for bamboo adaptation and will facilitate the research on bamboo breeding for stress tolerance.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109392"},"PeriodicalIF":6.1,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterization of the NAC transcription factors in carnation and function analysis of DcNAC41 involved in thermotolerance.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-06 DOI: 10.1016/j.plaphy.2024.109390

Mei Zhao, Ziyi Liu, Pengcheng Xue, Xiaojing Zhang, Xueli Wan

{"title":"Genomic characterization of the NAC transcription factors in carnation and function analysis of DcNAC41 involved in thermotolerance.","authors":"Mei Zhao, Ziyi Liu, Pengcheng Xue, Xiaojing Zhang, Xueli Wan","doi":"10.1016/j.plaphy.2024.109390","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109390","url":null,"abstract":"As pivotal regulators unique to plants, NAC family extensively orchestrate various life processes ranging from seed germination through growth and development to responses to environmental stresses. This study unraveled 71 NAC TFs in the carnation (Dianthus caryophyllus L.) genome, designated as DcNAC1 to DcNAC71, encoding amino acid sequences ranging from 80 to 718 residues. Subcellular localization predictions revealed a predominance of nuclear localization among these DcNACs. Phylogenetic analysis classified DcNACs into 14 distinct subgroups, each exhibiting similar gene structures and motifs. Promoter analysis highlighted the abundance of cis-regulatory elements (CREs) associated with plant growth and development regulation, hormone signaling, light response, and diverse stress responses, with stress-responsive CREs being the most prevalent, with at least one stress-responsive CRE detected in all DcNAC promoters. To assess their functional roles, 12 DcNACs, were randomly selected from different subgroups for expression profiling under heat, ABA, cold, and salt stress conditions, revealing distinct expression patterns for specific stress types. Notably, DcNAC41, which exhibited marked up-regulation under heat stress, was isolated and subsequently transformed into Arabidopsis. In heat-stressed conditions, transgenic Arabidopsis overexpressing DcNAC41 exhibited significant improvements in growth performance, survival rates, enhanced photosynthetic capacity, and strengthened ROS scavenging abilities. This study offers valuable insights into the comprehensive response of carnation DcNACs towards heat stress, particularly underscoring the potential of DcNAC41 as a promising candidate for enhancing thermotolerance in plants.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109390"},"PeriodicalIF":6.1,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsbZIP23 delays flowering by repressing OsMADS14 expression in rice.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-05 DOI: 10.1016/j.plaphy.2024.109389

Kunming Zhang, Chuyan Chen, Jun Miao, Bingyin Zou, Renyu Xu, Xiangbo Li, Xianfeng Li, Wenchen Tan, Zhiyun Gong, Chuandeng Yi, Guohua Liang, Yong Zhou

{"title":"OsbZIP23 delays flowering by repressing OsMADS14 expression in rice.","authors":"Kunming Zhang, Chuyan Chen, Jun Miao, Bingyin Zou, Renyu Xu, Xiangbo Li, Xianfeng Li, Wenchen Tan, Zhiyun Gong, Chuandeng Yi, Guohua Liang, Yong Zhou","doi":"10.1016/j.plaphy.2024.109389","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109389","url":null,"abstract":"Flowering time is a fundamental factor determining the global distribution and final yield of rice (Oryza sativa L.). The initiation of the floral transition process signifies the beginning of the reproductive phase. The florigens Heading Date 3a (Hd3a) and Rice Flowering Locus T 1 (RFT1) combine with GF14 proteins and OsFD-like basic leucine zipper (bZIP) transcription factors to form florigen activation/repressor complexes (FACs/FRCs) that regulate the transition to flowering. We herein report that a bZIP transcription factor (OsbZIP23) functions as a flowering repressor. Transgenic plants overexpressing OsbZIP23 exhibited delayed flowering, which was in contrast to the slightly early flowering of the osbzip23 mutants, under natural short-day and long-day conditions. Molecular and biochemical analyses indicated that OsbZIP23 can bind to the 5' untranslated region of OsMADS14 and suppress expression. Moreover, it delays the floral transition probably by interacting with OsFTL1/Hd3a/RFT1 and 14-3-3 proteins to form FRCs. Our findings have further elucidated the molecular mechanisms regulating the flowering time in rice.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109389"},"PeriodicalIF":6.1,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The DC1 domain protein Vacuoleless Gametophytes regulates stamen development in Arabidopsis.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-04 DOI: 10.1016/j.plaphy.2024.109372

Natalia L Amigo, Leonardo A Arias, Fernanda Marchetti, Sebastián D'Ippólito, Milagros Cascallares, Salvador Lorenzani, Jesica Frik, María Cristina Lombardo, María Cecilia Terrile, Claudia A Casalongue, Gabriela C Pagnussat, Diego F Fiol

{"title":"The DC1 domain protein Vacuoleless Gametophytes regulates stamen development in Arabidopsis.","authors":"Natalia L Amigo, Leonardo A Arias, Fernanda Marchetti, Sebastián D'Ippólito, Milagros Cascallares, Salvador Lorenzani, Jesica Frik, María Cristina Lombardo, María Cecilia Terrile, Claudia A Casalongue, Gabriela C Pagnussat, Diego F Fiol","doi":"10.1016/j.plaphy.2024.109372","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109372","url":null,"abstract":"Vacuoleless Gametophytes (VLG) is a DC1 domain protein that was initially characterized as essential for early female and male gametophytes development in Arabidopsis. However, VLG expression was also detected in stamens, pistils and other sporophytic tissues, implying a broader role for this protein. As homozygous insertional VLG lines resulted unviable, we generated Arabidopsis amiRNA VLG knock-down plants to study the role of VLG in sporophyte development. The phenotypic characterization of VLG knock-down plants showed reduced seed set and indehiscent anthers with shorter filaments and stigma exsertion. Moreover, amiRNA VLG knock-down plants displayed unbroken stomia and septa, markedly reduced endothecium lignification, diminished ROS accumulation, and lower transcript levels of genes involved in jasmonic acid and lignin biosynthesis. The indehiscent phenotype was rescued by exogenous application of either jasmonic acid or H2O2. Altogether, our results suggest that VLG is involved in lignin and jasmonic acid biosynthesis pathways, and that proper levels of VLG are required in the process that leads to stomium breakage and anther dehiscence. Our findings shed light on the mechanisms underlying stamen development and provide new insights into the roles of a DC1 domain protein in plant reproduction.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109372"},"PeriodicalIF":6.1,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triticain alpha represents the major active papain-like cysteine protease in naturally senescing and ozone-treated leaves of wheat.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-04 DOI: 10.1016/j.plaphy.2024.109380

Marien Havé, Christophe Espinasse, Betty Cottyn-Boitte, Ruben Puga-Freitas, Matthieu Bagard, Thierry Balliau, Michel Zivy, Seedhabadee Ganeshan, Ravindra N Chibbar, Jean-François Castell, Olivier Bethenod, Luis Leitao, Anne Repellin

{"title":"Triticain alpha represents the major active papain-like cysteine protease in naturally senescing and ozone-treated leaves of wheat.","authors":"Marien Havé, Christophe Espinasse, Betty Cottyn-Boitte, Ruben Puga-Freitas, Matthieu Bagard, Thierry Balliau, Michel Zivy, Seedhabadee Ganeshan, Ravindra N Chibbar, Jean-François Castell, Olivier Bethenod, Luis Leitao, Anne Repellin","doi":"10.1016/j.plaphy.2024.109380","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109380","url":null,"abstract":"Current background tropospheric ozone (O3) concentrations have significant adverse effects on wheat. O3 generally induces oxidative damages and premature leaf senescence leading to important yield losses. As leaf protein degradation and recycling is involved in both maintaining cell longevity during abiotic stresses and performing efficient nitrogen remobilization during senescence, we aimed to identify proteases involved in acidic endoproteolytic activities during natural and O3-induced leaf senescence in wheat. Field-grown plants of two winter wheat cultivars were exposed to ambient and semi-controlled chronic O3 concentrations, from pre-anthesis to grain harvest. Yield parameters were impacted by the most elevated O3 exposure for both cultivars. At the cellular level, our analysis revealed that both natural leaf senescence and O3 treatments induced a stimulation of acidic (pH 5.5) endoproteolytic activities, mostly due to papain-like cysteine proteases (PLCPs). Identification of active PLCPs using activity-based protein profiling (ABPP) revealed that Triticain α was the major active PLCP in senescing flag leaves and the only PLCP whose abundance increased with O3 stress, a result of positive transcriptional regulation. Our study provides novel insight into the implication of PLCP-mediated proteolysis in O3 sensitivity in a major crop.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109380"},"PeriodicalIF":6.1,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lead accumulation and concomitant reactive oxygen species (ROS) scavenging in Robinia pseudoacacia are dependent on nitrogen nutrition.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-04 DOI: 10.1016/j.plaphy.2024.109388

Zhuyuan Xia, Caixin Xue, Rui Liu, Qiuling Hui, Bin Hu, Heinz Rennenberg

{"title":"Lead accumulation and concomitant reactive oxygen species (ROS) scavenging in Robinia pseudoacacia are dependent on nitrogen nutrition.","authors":"Zhuyuan Xia, Caixin Xue, Rui Liu, Qiuling Hui, Bin Hu, Heinz Rennenberg","doi":"10.1016/j.plaphy.2024.109388","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109388","url":null,"abstract":"Heavy metal pollution combined with nitrogen (N) limitation is a major factor preventing revegetation of contaminated land. Woody N2-fixing legumes are a natural choice for phytoremediation. However, the physiological responses of woody legumes to lead (Pb) with low N exposure are currently unknown. In the present study, a common Robinia cultivar from Northeast China, inoculated and non-inoculated with rhizobia, was exposed to -Pb or + Pb at moderate (norN) or low N application (lowN). Our results showed that without inoculation, independent of N application, Pb taken up by the roots was allocated to the shoot and inhibited photosynthesis and biomass production. In non-inoculated Robinia, Pb-mediated oxidative stress resulted in reduced H2O2 scavenging as indicated by increased ascorbate peroxidase (APX) activity in the leaves and proline contents in the roots, independent of N application. Combined lowN∗Pb exposure significantly increased malondialdehyde (MDA) contents in roots and leaves and enhanced APX and dehydroascorbate reductase activities in leaves compared to individual Pb exposure. Rhizobia inoculation raised the abundance of nodules and promoted Pb uptake by roots. Under Pb exposure, inoculation with rhizobia reduced MDA contents, increased proline contents in leaves and roots and enhanced activity of nitrate reductase in the leaves, independent of N application. Under Pb exposure, nitrogenase activity of inoculated Robinia under low- and norN application were similar indicating that enhanced of N2-fixation at lowN was counteracted by Pb exposure. These results show that inoculation of Robinia with rhizobia can alleviate Pb toxicity at combined lowN and Pb exposure by reducing oxidative stress.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109388"},"PeriodicalIF":6.1,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From intercropping to monocropping: The effects of Pseudomonas strain to facilitate nutrient efficiency in peanut and soil.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-04 DOI: 10.1016/j.plaphy.2024.109378

Tianqi Wang, Kunguang Wang, Nanqi Wang, Dongming Cui, Shiqin Li, Qiaofang Lu, Yuanmei Zuo

{"title":"From intercropping to monocropping: The effects of Pseudomonas strain to facilitate nutrient efficiency in peanut and soil.","authors":"Tianqi Wang, Kunguang Wang, Nanqi Wang, Dongming Cui, Shiqin Li, Qiaofang Lu, Yuanmei Zuo","doi":"10.1016/j.plaphy.2024.109378","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109378","url":null,"abstract":"As an oilseed crop, the yield and quality of peanuts are severely constrained by nutrient deficiencies, particularly in calcareous soils in northern China. Maize-peanut intercropping is an effective strategy to enhance mineral nutrient efficiency in peanuts via plant-microbe interaction, but the underlying mechanisms remain elusive. Here, we conducted experiments using a Pseudomonas strain (Pse.IP6) with diverse beneficial characteristics, which was isolated from the rhizosphere of intercropped peanuts. Additionally, Pse.IP6 exhibits high phylogenetic similarity with the Amplicon Sequence Variants 48 (ASV48) which belongs to Pseudomonas and is positively correlated with Fe in plants and soil in intercropping. To confirm the plant growth-promoting potential of Pse.IP6 and its role in intercropping advantage, we constructed pot experiments. Results revealed that Pse.IP6 promoted shoot growth and root development, as well significantly enhanced SPAD value, net photosynthetic rate, stomatal conductance, and transpiration rate of peanut leaves. Moreover, the application of Pse.IP6 resulted in a notable accumulation of nitrogen (N), phosphorus (P), and potassium (K) in shoot and active iron (Fe) in leaves, accompanied by an increased K-N ratio. The primary reason for the nutrient promotion is the enhancement of the bioavailability of nitrate, ammonium, P, K, and Fe in the rhizosphere. Collectively, our findings demonstrate that Pse.IP6, enriched in intercropping peanut, is a plant growth-promoting bacteria, represented by transferring the intercropping advantage on nutrients activation to monocropping peanuts. Our results offer insights into plant-rhizobacteria interaction mechanisms and therefore provide a rhizobacteria-based pathway to improve nutrient efficiency and productivity of crops.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109378"},"PeriodicalIF":6.1,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VvNAC17, a grape NAC transcription factor, regulates plant response to drought-tolerance and anthocyanin synthesis.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-12-04 DOI: 10.1016/j.plaphy.2024.109379

Zi-Lan Jin, Wan-Ni Wang, Qiong Nan, Jia-Wen Liu, Yan-Lun Ju, Yu-Lin Fang

{"title":"VvNAC17, a grape NAC transcription factor, regulates plant response to drought-tolerance and anthocyanin synthesis.","authors":"Zi-Lan Jin, Wan-Ni Wang, Qiong Nan, Jia-Wen Liu, Yan-Lun Ju, Yu-Lin Fang","doi":"10.1016/j.plaphy.2024.109379","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109379","url":null,"abstract":"NAC transcription factors are unique to plants and play a role in stress. In this study, the VvNAC17 gene was isolated from grapes, and tested the functions of VvNAC17 under drought stress. The expression level of VvNAC17 in callus could be highly induced by drought stress. VvNAC17 overexpression in callus conferred drought-resistant phenotypes with lower MDA content, higher antioxidant enzyme activity (CAT, POD, SOD), higher monomer anthocyanin content, and higher expression levels of some drought-related genes (VvDREB1A, VvDREB2A, VvDREB2D, VvRD29A, VvPIN5) and anthocyanin-biosynthesis-related genes (VvUFGT, VvANS, VvANR, VvDFR,VvLAR). Meanwhile, the Y1H and Dual-LUC assays showed that VvNAC17 could activate VvDREB1A and VvUFGT expression by binding to its promoter. Futhermore, RNA-seq showed that VvNAC17 can affect grape growth and development by affecting the photosynthesis and metabolism of some macromolecules. Taken together, the NAC transcription factor VvNAC17 could positively regulates drought-tolerance. VvNAC17 is a promising candidate for improving drought resistance in grape.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"219 ","pages":"109379"},"PeriodicalIF":6.1,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0