Plant Physiology and Biochemistry最新文献

{"title":"Real-time monitoring of stromal NADPH levels in Arabidopsis using a metagenome-derived NADPH-binding fluorescent protein","authors":"Jin Hoon Jang ,&nbsp;Da Been Kim ,&nbsp;Yeonsu Choi ,&nbsp;Roshanzadeh Amir ,&nbsp;Dae-Eun Cheong ,&nbsp;Hea-Jong Chung ,&nbsp;Sun-Hee Ahn ,&nbsp;Geun-Joong Kim ,&nbsp;Dong Wook Lee ,&nbsp;Ok Ran Lee ,&nbsp;Eung-Sam Kim","doi":"10.1016/j.plaphy.2024.109260","DOIUrl":"10.1016/j.plaphy.2024.109260","url":null,"abstract":"<div><div>The light irradiation to the plant chloroplasts drives NADPH and ATP synthesis in the stroma via the electron transport chains within the thylakoid membranes. Conventional methods for assessing photosynthetic light reactions are often invasive or require specific conditions. While detection markers do not significantly affect plant growth itself, developing a method for the real-time and non-invasive detection of NADPH is a highly impactful and important research area in plant physiology and biochemistry. This study introduces a genetically encoded NADPH-binding blue fluorescent protein (mBFP) targeted to the chloroplast stroma or thylakoid membrane in <em>Arabidopsis thaliana</em> and <em>Nicotiana benthamiana</em>. Using two-photon microscopy, we monitored real-time stromal NADPH levels in transgenic leaves of Arabidopsis in response to light exposure. A mutant mBFP construct targeted to the thylakoid membrane allowed us to detect the stromal NADPH levels in real time under different light conditions. This <em>in planta</em> biosensor provides a non-invasive tool for studying photosynthetic responses to light more quantitatively and holds potential for optimizing light conditions in controlled-environment agriculture, such as indoor vertical farms, to improve crop productivity.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Over-expression of SiADCL1 in Arabidopsis modulates folate and amino acid metabolism to impact on flowering time","authors":"Yue Zhao ,&nbsp;Jiongyu Hao ,&nbsp;Yihan Men ,&nbsp;Jiaqi Yuan ,&nbsp;Cui Ma ,&nbsp;Yang Yang ,&nbsp;Yuanhuai Han ,&nbsp;Luis A.J. Mur ,&nbsp;Zhaoxia Sun ,&nbsp;Siyu Hou","doi":"10.1016/j.plaphy.2024.109253","DOIUrl":"10.1016/j.plaphy.2024.109253","url":null,"abstract":"<div><div>Foxtail millet is a C₄ crop rich in folate (FA). This study explores the roles of the 4-amino-4-deoxychorismate lyase (<em>ADCL</em>) – a member of the transaminase IV group of enzymes – in FA metabolism and conferred phenotypes. Phylogenetic comparisons identified diversity in the transaminase IV/<em>ADCL</em> gene family in the foxtail millet genome which was associated with genomic duplications. Molecular docking studies suggested that <em>SiADCL1</em> bound most strongly to aminodeoxychorismate (<em>ADC</em>) and most likely had the highest catalytic activities. <em>SiADCL1</em> which was highly expressed in roots, peduncles and flag leaves. Over-expression of <em>SiADCL1</em> in Arabidopsis significantly increased total FA content (1.14–1.84 fold) and this was linked to a delayed flowering time. Metabolomic and transcriptomic characterization of the derived over-expression lines, found that FA promotes the change of methylation-related genes, ethylene synthesis, amino acid metabolism and flowering-related genes. This study revealed a potential gene coexpression network linked with FA and targeted key genes that could be exploited in foxtail millet breeding programs.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A R2R3-MYB transcription factor, FeR2R3-MYB, positively regulates anthocyanin biosynthesis and drought tolerance in common buckwheat (Fagopyrum esculentum)","authors":"Yirou Luo ,&nbsp;Xiaoyu Xu ,&nbsp;Lanfeng Yang ,&nbsp;Xudong Zhu ,&nbsp;Yingbiao Du ,&nbsp;Zhengwu Fang","doi":"10.1016/j.plaphy.2024.109254","DOIUrl":"10.1016/j.plaphy.2024.109254","url":null,"abstract":"<div><div>The R2R3-MYB transcription factors (TFs) play a crucial role in regulating plant secondary metabolism and abiotic stress responses, yet they are still poorly understood in common buckwheat (Fagopyrum esculentum), a valuable minor grain crop resource. In this study, a candidate gene, <em>FeR2R3-MYB</em>, was cloned from the anthocyanin-rich common buckwheat variety ‘QZZTQ’. FeR2R3-MYB was found to contain two MYB DNA-binding domains and be located at the nucleus with transcriptional activation activity. Molecular analysis indicated that FeR2R3-MYB is predominantly expressed in flowering tissue and is highly responsive to environmental factors such as light, drought, and cold. In addition, the promoter of FeR2R3-MYB showed a positive correlation with fragment length. Further functional analysis suggested that FeR2R3-MYB not only participates in the anthocyanin biosynthetic pathway by interacting with leucoanthocyanidin reductase (FeLAR), but also enhances drought tolerance in common buckwheat. To sum up, FeR2R3-MYB exhibits positive effects on both pigment production (e.g., anthocyanin) and abiotic stress resistance, providing valuable insights for future research in buckwheat molecular breeding and resource development.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metarhizium anisopliae (MetA1) seed priming improves photosynthesis, growth, plant defense and yield of wheat under drought stress","authors":"Mahjabin Ferdaous Mim ,&nbsp;Md. Zahid Hasan Chowdhury ,&nbsp;Md. Motiar Rohman ,&nbsp;Atofa Naz ,&nbsp;Ashkar-Ul-Alam Bhuiyan ,&nbsp;Mohammed Mohi-Ud-Din ,&nbsp;Md. Ashraful Haque ,&nbsp;Shah Mohammad Naimul Islam","doi":"10.1016/j.plaphy.2024.109239","DOIUrl":"10.1016/j.plaphy.2024.109239","url":null,"abstract":"<div><div>Drought stress mitigation by endophytic microorganisms has the potential to enhance crop resilience. This research investigated the effects of <em>M. anisopliae</em> isolate MetA1 (MA) on drought tolerance in wheat plants by evaluating physiological, morphological, biochemical, and yield characteristics of two wheat genotypes, one being drought-susceptible (BR20) and another having drought withstanding capacities (BR28) in a pot experiment under moderate and severe drought conditions. Under drought conditions, root colonization by <em>M. anisopliae</em> ranged from 33.33 to 66.67%. <em>M. anisopliae</em> treated plants increased the plant photosynthetic capacities by increasing photosynthetic pigments, Phi2, gH⁺, vH⁺, LEF, ECSt, leaf thickness and decreasing PhiNO, PhiNPQ, NPQt and leaf angle mostly for both genotypes, which contributed significant improvement of plant biomass in drought conditions. Exceptionally, the seed primed with <em>M. anisopliae</em> noticeably improved root length (by up to 17.6%) under drought conditions corroborated with the plant's drought mitigating approach. The stress induced malondialdehyde and hydrogen peroxide levels were dropped significantly by improving enzymatic antioxidants, such as peroxidase, ascorbate peroxidase, superoxide dismutase, catalase, and glutathione S-transferase in both <em>M. anisopliae</em> -primed genotypes. Also, proline content increased in the leaves of <em>M. anisopliae</em> treated plant which indicates better osmotic adjustment. Finally, <em>M. anisopliae</em> seed priming increased yield and yield characteristics of both genotypes in drought as well as non-drought situations with 1000-grain weight improving by up to 41.77% under severe drought. The study proposes a further investigation of <em>M. anisopliae</em>'s effect in field settings and its applicability to other crops. Collectively, these findings emphasize the practical potential of <em>M. anisopliae</em> seed priming in boosting wheat production under water-limited circumstances, presenting a realistic technique for minimizing the effect of drought on global food security.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Up-regulation of nitrogen metabolism and chlorophyll biosynthesis by hydrogen sulfide improved photosystem photochemistry and gas exchange in chromium-contaminated bean (Phaseolus vulgaris L.) plants","authors":"Oussama Kharbech ,&nbsp;Yathreb Mahjoubi ,&nbsp;Marwa Boutar ,&nbsp;Wahbi Djebali ,&nbsp;Abdelilah Chaoui","doi":"10.1016/j.plaphy.2024.109211","DOIUrl":"10.1016/j.plaphy.2024.109211","url":null,"abstract":"<div><div>Hydrogen sulfide (H₂S) is considered as plant growth promoter under heavy metal stress, though its specific effects on photosynthesis are rarely explored. This study investigates the protective effects of exogenous H₂S donor sodium hydrosulfide (NaHS) on chlorophyll metabolism and photosystem II (PSII) function in 24-day-old bean plants exposed to 10 μM chromium (Cr) stress. Sodium hydrosulfide (100 μM) reduced Cr accumulation in both roots and leaves, leading to restored plant growth. Concomitantly, H₂S mitigated Cr-induced oxidative damages by decreasing reactive oxygen species levels and further enhancing antioxidant scavenging activities. This resulted in significant reductions in Cr-elevated leaf pheophytin and chlorophyllide levels by 59% and 67%, respectively. Furthermore, NaHS application increased levels of porphyrin and its precursor, 5-aminolevulinic acid (5-ALA), in Cr-stressed bean. The up-regulation in chlorophyll biosynthesis was associated with enhanced activities of glutamine synthetase and glutamate synthase, essential for glutamate (precursor of 5-ALA) production, as well as nitrate and nitrite reductase, leading to increased nitric oxide generation. Under Cr stress, H₂S significantly improved the electron transport rate, effective quantum yield of PSII, and photochemical quenching by 112%, 53%, and 38%, respectively, while reducing non-photochemical quenching by 50%. Furthermore, H₂S promoted net CO₂ assimilation and photosynthesis at saturating light, respectively, while reducing stomatal conductance and transpiration to maintain water balance. Exogenous H₂S restored respiration, as indicated by increased light saturation and compensation points in Cr-treated plants. Overall, these findings indicate that H₂S regulates photosynthesis in Cr-stressed bean by modulating nitrogen and chlorophyll metabolism, thereby optimizing PSII efficiency and gas exchange.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced photorespiratory and TCA pathways by elevated CO2 to manage ammonium nutrition in tomato leaves","authors":"Izargi Vega-Mas,&nbsp;Daniel Marino,&nbsp;Marlon De la Peña,&nbsp;Teresa Fuertes-Mendizábal,&nbsp;Carmen González-Murua,&nbsp;José María Estavillo,&nbsp;María Begoña González-Moro","doi":"10.1016/j.plaphy.2024.109216","DOIUrl":"10.1016/j.plaphy.2024.109216","url":null,"abstract":"<div><div>Plants grown under exclusive ammonium (NH₄⁺) nutrition have high carbon (C) demand to sustain proper nitrogen (N) assimilation and energy required for plant growth, generally impaired when compared to nitrate (NO₃⁻) nutrition. Thereby, the increment of the atmospheric carbon dioxide (CO₂) concentration, in the context of climate change, will potentially allow plants to better face ammonium nutrition. In this work, tomato (<em>Solanum lycopersicum</em> L.) plants were grown under ammonium or nitrate nutrition in conditions of ambient (aCO₂, 400 ppm) or elevated CO₂ (eCO_2, 800 ppm) atmosphere. Elevated CO₂ increased photosynthesis rate and tomato shoot growth regardless of the N source. In the case of NH₄⁺-fed leaves the positive effect of elevated CO₂ occurred despite of the high tissue NH₄⁺ accumulation. Under eCO₂ ammonium nutrition triggered, among others, the modulation of genes related to C provision pathways (including carbonic anhydrase and glyoxylate cycle), antioxidant response and cell membranes protection. The enhanced photosynthate production at eCO₂ facilitated C skeleton provision through the TCA cycle and anaplerotic pathways to promote amino acid synthesis. Moreover, photorespiratory activity was stimulated by eCO₂ and contributed to yield serine as additional sink for NH₄⁺ excess. Overall, these changes denote a connection between the respiratory and the photorespiratory pathways linked to ammonium nutrition. This metabolic strategy may allow crops to grow efficiently using ammonium as fertilizer in a future climate change scenario, while mitigating N losses.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of TkWRKY33: A key regulator in drought-induced natural rubber synthesis in Taraxacum kok-saghyz","authors":"Yulin Wu ,&nbsp;Yaxin Liu ,&nbsp;Yunchuan Zhang ,&nbsp;Gaoquan Dong ,&nbsp;Jie Yan ,&nbsp;Hao Zhang","doi":"10.1016/j.plaphy.2024.109232","DOIUrl":"10.1016/j.plaphy.2024.109232","url":null,"abstract":"<div><div>WRKY proteins, which form a transcription factor superfamily that responds to jasmonic acid (JA) signals, regulate various developmental processes and stress responses in plants, including <em>Taraxacum kok-saghyz</em> (TKS). TKS serves as an ideal model plant for studying rubber production and lays the foundation for a comprehensive understanding of JA-mediated regulation of natural rubber synthesis. In the present study, we screened and identified a valuable transcription factor, TkWRKY33, based on transcriptome data from TKS in response to JA. We investigated its role in the regulation of natural rubber synthesis within the JA signaling pathway and its function in response to drought stress. Through protein-protein interactions and transcriptional regulation analysis, we found that TkWRKY33 may regulate natural rubber synthesis through the JA-TkMPK3-TkWRKY33-(TkGGPS5/TkACAT8) cascade pathway, possibly by participating in JA-activated mitogen-activated protein kinase (MAPK) signaling. Overexpression of TkWRKY33 in tobacco, along with functional analysis of drought resistance and comparative analysis of natural rubber content after drought stress, revealed that TkWRKY33 not only enhances plant drought resistance by regulating the expression of genes related to reactive oxygen species (ROS) scavenging through the JA signaling pathway, but also has a close relationship with the signal transduction pathway mediated by the JA hormone in regulating natural rubber synthesis.</div><div>The TkWRKY33 is recognized as a valuable transcription factor, which likely plays a role in regulating natural rubber biosynthesis through the JA-activated MAPK cascade signaling pathway JA-TkMPK3-TkWRKY33-(TkGGPS5/TkACAT8).</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foliar application of carbon dots enhances nitrogen uptake and assimilation through CEPD1-dependent signaling in plants","authors":"Zhiyuan Pan ,&nbsp;Huihui Zang ,&nbsp;Yanjuan Li ,&nbsp;Xiao Wang ,&nbsp;Nan Xia ,&nbsp;Chong Liu ,&nbsp;Zongyun Li ,&nbsp;Yonghua Han ,&nbsp;Zhonghou Tang ,&nbsp;Jian Sun","doi":"10.1016/j.plaphy.2024.109229","DOIUrl":"10.1016/j.plaphy.2024.109229","url":null,"abstract":"<div><div>The use of nitrogen (N) fertilizers increases crop yield, but the accumulation of residual N in agricultural soils poses significant environmental risks. Improving the N use efficiency (NUE) of crops can help reduce N pollution. While nanomaterials have been shown to enhance crop agronomic traits, more research is needed to clarify the regulatory mechanisms involved. In this study, foliar spraying of carbon dots (CDs, 1 mg mL⁻¹) derived from <em>Salvia miltiorrhiza</em> increased the activity of plasma membrane H⁺-ATPase in <em>Arabidopsis thaliana</em> roots, promoting the uptake, transport, and assimilation of NO₃⁻ and NH₄⁺. The upregulation of N metabolism-related genes, such as <em>AtAMTs</em> and <em>AtNRTs</em>, was also observed in <em>A. thaliana</em> roots. Transcriptome analysis suggested that this regulatory effect is mediated by the shoot-to-root mobile polypeptide CEPD1 (C-terminally encoded peptide DOWNSTREAM 1) signaling pathway. Additionally, foliar application of CDs increased the NUE of sweetpotato (<em>Ipomoea batatas</em> (L.) Lam.) from 2.5% to 8.1%. The upregulation of genes such as <em>CEPD1</em> in leaves was observed following CDs application under different N conditions. Finally, foliar spraying of CDs significantly increased field yield and enhanced tolerance to low N stress in sweetpotato. Overall, this study demonstrated that foliar application of CDs improved NUE in plants through CEPD1-dependent signaling.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal influence on tomato fruit metabolome profile: Implications for ABA signaling in multi-stress resilience","authors":"Miriam Pardo-Hernández ,&nbsp;Leilei Zhang ,&nbsp;Luigi Lucini ,&nbsp;Rosa M. Rivero","doi":"10.1016/j.plaphy.2024.109234","DOIUrl":"10.1016/j.plaphy.2024.109234","url":null,"abstract":"<div><div>The increasing effects of climate change are leading to an increase in the number and intensity of extreme events, making it essential to study how plants respond to various stresses occurring simultaneously. A crucial regulator of plant responses to abiotic stress is abscisic acid (ABA), as its accumulation in response to stress leads to transcriptomic and metabolomic changes that contribute to plant stress tolerance. In the present study, we investigated how ABA, stress conditions (salinity, water deficit and their combination) and seasons (autumn-winter and spring-summer) regulate tomato fruit yield and metabolism using tomato wild type (WT) and the ABA-deficient <em>flacca</em> mutant (<em>flc</em>) under stress conditions in cold and warm seasons. Our results showed that the applied stresses did not have the same effect in the warm season as in the cold season. In WT plants, the levels of other flavonoids, lignans and other polyphenols were higher in summer fruits, whereas the levels of anthocyanins, flavanols, flavonols, phenolic acids and stilbenes were higher in winter fruits. Furthermore, the significant increase in anthocyanins and flavonols was associated with the combination of salinity + water deficit in both seasons. Additionally, under certain conditions, <em>flc</em> mutants showed an enrichment of the superclasses of benzenoids and organosulphur compounds. The synthesis of phenolic compounds in <em>flc</em> fruits was also significantly different compared to WT plants. Thus, the metabolic profile of tomato fruits varies significantly with endogenous ABA levels, season of cultivation and applied stress treatments, highlighting the multifactorial nature of plant responses to combined environmental factors.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142527463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of putative genes for caffeoylated flavonoid glycoside biosynthesis in Pseudognaphalium affine","authors":"Yongkang Li ,&nbsp;Qing Li ,&nbsp;Dongtian Liu ,&nbsp;Zongtai Wu ,&nbsp;Lianna Sun ,&nbsp;Wansheng Chen ,&nbsp;Ying Xiao","doi":"10.1016/j.plaphy.2024.109233","DOIUrl":"10.1016/j.plaphy.2024.109233","url":null,"abstract":"<div><div><em>Pseudognaphalium affine</em> (D. Don) Anderberg, commonly found in East Asia, has extensive applications as both a traditional medicine and a vegetable in China. The caffeoylated flavonoid glycosides produced by <em>P. affine</em> exhibit remarkable anti-complement activities. Although these compounds have potential therapeutic value, the biosynthetic pathway responsible for their production remains largely unknown. To elucidate the key catalytic steps involved in caffeoylated flavonoid glycoside biosynthesis, we conducted a comprehensive analysis of the full-length transcriptome of <em>P. affine</em>. Further phylogenetic tree analysis predicted potential UDP glycosyltransferase (UGT) and BAHD acyltransferase (BAHD-AT) related with caffeoylated flavonoid glycoside biosynthesis. Subsequently, enzyme assay led to the discovery of <em>PaUGT23</em> as a key enzyme responsible for the glycosylation of hydroxy groups in flavonoids, resulting in the formation of luteolin-4′-<em>O</em>-glucoside, luteolin-7-<em>O</em>-glucoside, quercetin-4′-<em>O</em>-glucoside, quercetin-7-<em>O</em>-glucoside, and apigenin-7-<em>O</em>-glucoside, while <em>Pa</em>BAHD21 was found to catalyze the caffeoylation of flavonoid glycosides, resulting in the formation of luteolin 4′-<em>O</em>-β-D-(6″-<em>E</em>-caffeoyl)-glucopyranoside, quercetin 4′-<em>O</em>-β-D-(6″-<em>E</em>-caffeoyl)-glucopyranoside, apigenin 4′-<em>O</em>-β-D-(6″-<em>E</em>-caffeoyl)-glucopyranoside and apigenin 7-<em>O</em>-β-D-(6″-<em>E</em>-caffeoyl)-glucopyranoside. Moreover, their catalytic activities were verified <em>in vivo</em> by transient transfection experiment. This study presents the first comprehensive analysis of the full-length transcriptome in <em>P. affine</em>, providing significant insights into the biosynthesis and accumulation mechanisms of bioactive caffeoylated flavonoid glycosides.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}