Plant Physiology and Biochemistry最新文献

{"title":"Enhanced bacoside synthesis in Bacopa monnieri plants using seed exudates from Tamarindus indica","authors":"Titir Guha ,&nbsp;Ekta Bhattacharya ,&nbsp;Madhurima Dutta ,&nbsp;Anisha Dutta ,&nbsp;Moumita Dandapat ,&nbsp;Rahul Bose ,&nbsp;Suparna Mandal Biswas","doi":"10.1016/j.plaphy.2024.109287","DOIUrl":"10.1016/j.plaphy.2024.109287","url":null,"abstract":"<div><div>Diverse allelochemicals are released from different plant parts via leaching, exudation, volatilization, etc., which can induce either stimulatory or inhibitory effects depending on the target plant species. Very few reports provide details about allelopathic interaction through seed exudates. Since <em>Tamarindus indica</em> L. seed exudate (TSE) has been known to exhibit growth stimulatory effect on lettuce, radish, and sesame, in the present study we have evaluated its role in regulating the secondary metabolism of an over-exploited medicinal herb, <em>Bacopa monnieri</em> (L.) Pennel. The bacoside biosynthesis rate of <em>B. monnieri</em> is quite low in comparison to its increasingly high demands in the pharmaceutical industry. Currently, researches are aimed towards enhancing the biosynthesis of this secondary metabolite <em>in planta</em> by utilizing external stress factors. Presently, 7-day-old <em>B. monnieri</em> seedlings were treated with 1:16, 1:8, 1:4, 1:3, and 1:2 (seed weight: water) TSE. Maximum upregulation of secondary metabolite contents was found in the 1:4 (seed weight: water) TSE treatment set. This TSE treatment also enhanced H₂O₂ and salicylic acid production leading to the upregulation of the genes related to the MVA pathway (<em>BmAACT</em>, <em>BmHMGR</em>, <em>BmMDD</em>, <em>BmSQS, and BmBAS</em>) which are responsible for bacoside biosynthesis and 1.7-fold higher bacoside level was found in TSE treated set compared to control. LC-HRMS analysis of TSE confirmed the presence of alkaloid (lupanine), phenol (chlorogenic acid), and organic acid (mucic acid), which are identified as potential allelochemicals responsible for modulating the secondary metabolism of <em>B. monnieri</em>. Thus, this study highlights a sustainable approach towards enhancing bacoside production <em>in planta</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109287"},"PeriodicalIF":6.1,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated quality, physiological and transcriptomic analysis reveals mechanisms of kiwifruit response to postharvest transport vibrational stress","authors":"Chenxu Zhao ,&nbsp;Linlin Cheng ,&nbsp;Yurong Guo ,&nbsp;Wei Hui ,&nbsp;Junpeng Niu ,&nbsp;Shujie Song","doi":"10.1016/j.plaphy.2024.109285","DOIUrl":"10.1016/j.plaphy.2024.109285","url":null,"abstract":"<div><div>The ‘Xuxiang’ kiwifruit, a leading cultivar in China known for its high quality and yield, experiences quality degradation due to vibration stress during postharvest transportation. This study simulated the postharvest transportation vibrations of ‘Xuxiang’ kiwifruits to investigate the effects on the fruit quality and physiology. Different vibration intensities (0.26, 0.79, and 1.5 m s⁻²) and durations (0, 24, 48, 72, and 96 h) were applied to analyze the quality, physiological and transcriptomic changes of fruits after vibration stress, as well as the association between quality deterioration, gene networks, and key genes. Results indicated that vibration stress significantly accelerated the deterioration of fruit quality and induced physiological changes. As vibration intensity and duration increased, there was a rapid decrease in fruit firmness and an increase in weight loss, soluble solid content, relative conductivity, ethylene production, respiratory rate, and malondialdehyde levels. The most severe deterioration in fruit quality occurred at a vibration intensity of 1.5 m s⁻². Transcriptome sequencing analysis was conducted on samples from different durations of exposure to the 1.5 m s⁻² vibration intensity. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses identified key genes associated with ethylene metabolism and softening. Weighted Gene Co-Expression Network Analysis (WGCNA) and correlation analysis further determined that 24 of these genes were regulated by vibrational stress, impacting ethylene metabolism and cell wall degradation. Vibration stress induced changes in genes related to ethylene metabolism and cell wall degradation, promoting lipid peroxidation and respiratory changes, which compromise cell membrane integrity and lead to quality deterioration. Compared with untreated fruits, vibration stress caused the quality deterioration, physiological changes and transcriptional regulation of kiwifruits, indicating that kiwifruits respond to vibration stress through multiple aspects. It proposes a fresh outlook on the understanding of the mechanism of transport vibration stress and further illustrates the importance of monitoring vibration intensity and duration as well as reducing vibration.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109285"},"PeriodicalIF":6.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MYB-1 regulates anthocyanin biosynthesis in Magnolia wufengensis","authors":"Xian-ping Liu ,&nbsp;Min Luo ,&nbsp;Xiu-qi Liu ,&nbsp;Liu-yan Hao ,&nbsp;Chen Zhu ,&nbsp;Li Wang ,&nbsp;Lv-yi Ma","doi":"10.1016/j.plaphy.2024.109283","DOIUrl":"10.1016/j.plaphy.2024.109283","url":null,"abstract":"<div><div>Anthocyanin is an essential pigment in all major horticultural crops especially in ornamental trees. <em>Magnolia wufengensis</em> (new species of <em>Magnolia</em>) with red color flower was recently found as a popular species for ornamental use, but anthocyanin synthesis and regulation in <em>M</em>. <em>wufengensis</em> are poorly understood. Herein, transcriptome analysis was used to decipher the gene network associated with anthocyanin biosynthesis. An R2R3-like MwMYB-1 transcription factor was found. MwMYB-1 overexpression resulted in anthocyanin accumulation in tobacco and Arabidopsis. MwMYB-1 worked independently rather than forming a protein complex with bHLH or WD40 protein. According to MwMYB-1 DAP-seq analysis in Arabidopsis, the MwMYB-1 transcription factor preferred to bind the “AAGAGAG” motif (DREME-5) in the third exon of the <em>AtMYB75</em> gene. The yeast one hybrid assay and transcription activity assay further confirmed this. Thus, MwMYB-1 activated <em>AtMYB75</em> gene expression and conducted cascade amplification of anthocyanin biosynthesis. Taken together, our findings provide a novel understanding of anthocyanin biosynthesis regulation in <em>M. wufengensis</em> and can be used to promote agronomic trait improvement in tree species.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109283"},"PeriodicalIF":6.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide characterization of the DIR gene family in sesame reveals the function of SiDIR21 in lignan biosynthesis","authors":"Huan Li ,&nbsp;Fengduo Hu ,&nbsp;Jianglong Zhou,&nbsp;Li Yang,&nbsp;Donghua Li,&nbsp;Rong Zhou,&nbsp;Ting Zhou,&nbsp;Yanxin Zhang,&nbsp;Linhai Wang,&nbsp;Jun You","doi":"10.1016/j.plaphy.2024.109282","DOIUrl":"10.1016/j.plaphy.2024.109282","url":null,"abstract":"<div><div>Furofuran-type lignans, mainly sesamin and sesamolin, are the most representative functional active ingredients in sesame (<em>Sesamum indicum</em> L.). Their exceptional antioxidant properties, medicinal benefits, and health-promoting functions have garnered significant attention. Dirigent (DIR) proteins, found in vascular plants, are crucial for the biosynthesis of secondary metabolites, like lignans, and essential for responding to abiotic and biotic stresses. Despite their importance, they have yet to be systematically analyzed, especially those involved in lignan synthesis in sesame. This study unveiled 44 DIR genes in sesame. Phylogenetic analysis categorized these SiDIRs into five subgroups (DIR-a, DIR-b/d, DIR-e, DIR-f, and DIR-g), aligning with conserved motifs and gene structures analyses. Expression analysis unveiled distinct tissue-specific and hormone-responsive expression patterns among the SiDIR gene family members. Particularly, <em>SiDIR21</em>, a member of the DIR-a subgroup, exhibited robust expression in lignan-accumulating tissues and consistently high expression levels in germplasm during seed development with high sesamin content. Furthermore, <em>SiDIR21</em> overexpression in hairy roots significantly increased sesamin and sesamolin contents, confirming its role in lignan synthesis. Overall, our study offers a valuable resource for exploring SiDIRs’ functions and the lignan biosynthesis pathway in sesame.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109282"},"PeriodicalIF":6.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histone demethylase JMJ713 interaction with JMJ708 modulating H3K36me2, enhances rice heat tolerance through promoting hydrogen peroxide scavenging","authors":"Jiaxin Chai ,&nbsp;Xiangyang Gu ,&nbsp;Pengyu Song ,&nbsp;Xinzhou Zhao ,&nbsp;Yingjie Gao ,&nbsp;Haiqi Wang ,&nbsp;Qian Zhang ,&nbsp;Tingting Cai ,&nbsp;Yutong Liu ,&nbsp;Xiaoting Li ,&nbsp;Tao Song ,&nbsp;Zhengge Zhu","doi":"10.1016/j.plaphy.2024.109284","DOIUrl":"10.1016/j.plaphy.2024.109284","url":null,"abstract":"<div><div>The Earth is currently undergoing rapid warming cause of the accumulation in greenhouse gas emissions into the atmosphere and the consequent rise in global temperatures. High temperatures can bring the effects on rice development and growth and thereby decrease rice yield. In this study, we have identified that both JMJ713 and JMJ708 possess distinct histone demethylase activities. Specifically, JMJ713 modulates the levels of H3K36me2 while JMJ708 alters H3K9me3. Additionally, we have observed an interaction between JMJ713 and JMJ708, which collectively modify the level of H3K36me2. Furthermore, our findings demonstrate that JMJ713 plays an essential role to heat stress responses in rice (<em>Oryza sativa</em>). The overexpression of <em>JMJ713</em> enhances heat tolerance in rice, whereas <em>JMJ7</em>13 RNA interference rice lines exhibit increased sensitivity to heat. Further investigations revealed that overexpression of <em>JMJ713</em> activated catalase (CAT) and peroxidase (POD) activities by mitigating excessive accumulation of reactive oxygen species (ROS) caused by heat stress. Interestingly, the setting rates of <em>JMJ7</em>13 RNA interference lines decreased in comparing to wild-type, indicating that <em>JMJ7</em>13 might play a crucial role in the rice seed development stage as well. Collectively, this study not only highlights JMJ713 is involved in heat stress responses but also provides insights into the conserved Fe(Ⅱ) and α-ketoglutarate (KG) binding residues are crucial for the demethylase activity of JMJ713, as well as JMJ713 interacts with JMJ708 to jointly regulate the levels of H3K36me2.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109284"},"PeriodicalIF":6.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas putida triggers phosphorus bioavailability and P-transporters under different phosphate regimes to enhance maize growth","authors":"Tanya Singh ,&nbsp;Nikita Bisht ,&nbsp;Mohd Mogees Ansari ,&nbsp;Puneet Singh Chauhan","doi":"10.1016/j.plaphy.2024.109279","DOIUrl":"10.1016/j.plaphy.2024.109279","url":null,"abstract":"<div><div>The decline of available phosphorus in soil due to anthropogenic activities necessitates utilizing soil microorganisms that influence soil phosphorus levels. However, the specific mechanisms governing their interaction in <em>Zea mays</em> under diverse phosphate regimes remain largely unknown. The present study investigated the dynamics of phosphorus solubilization and the impact of organic acid supplementation in combination with the beneficial rhizobacterium <em>Pseudomonas putida</em> (RA) on maize growth under phosphorus-limiting and unavailable conditions. HPLC analysis revealed gluconic acid as the primary organic acid (OA) produced by <em>P. putida</em> across all three conditions (P-sufficient, P-limiting, and P-unavailable), with the highest production occurring under P-limiting conditions. The study evaluates the effects of RA, OA, and OA + RA on plant growth parameters under P-limiting and insufficient conditions, revealing significant alterations in growth and biochemical parameters (<em>P =</em> 0.05) compared to their respective untreated controls. Additionally, plants treated with organic acids and bacterial inoculation show increased phosphorus concentrations in both roots and shoots. Gene expression analysis of key phosphorus transporter genes (<em>PHT1, PHO1, PTF, PHF1</em>) further supports the role of organic acids and bacterial inoculation in enhancing phosphorus uptake. In conclusion, our study affirms that the secretion of gluconic acid by RA and its plant growth-promoting properties boost phosphorus uptake and maize growth by increasing phosphorus availability and influencing the expression of phosphorus transport-related genes.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109279"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the synergistic effects of nano-zinc and biochar in mitigating aluminum toxicity in soybeans","authors":"Enxi Zhang ,&nbsp;Kun Liu ,&nbsp;Suwen Liang ,&nbsp;Lingrui Liu ,&nbsp;Hai Nian ,&nbsp;Tengxiang Lian","doi":"10.1016/j.plaphy.2024.109275","DOIUrl":"10.1016/j.plaphy.2024.109275","url":null,"abstract":"<div><div>Aluminum (Al) toxicity limited root growth by reducing nutrient translocation and promoting reactive oxygen species (ROS) accumulation, particularly in soybean. The endophyte of root could be modified by plant metabolites, which could potentially alter the tolerance to environmental toxicity of plants in acidic-Al soils. To explore how they help soybean mitigate Al toxicity by altering root endophytes, zinc oxide nanoparticles (ZnO NPs) at doses of 0, 30, 60, 90 mg/kg and 2% biochar (BC) were selected as bio modifiers, and Al₂(SO₄)₃ at 19 mg/kg was used to simulate Al toxicity. We analyzed root endophytes and metabolites by high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS). We found that ZnO NPs with BC could bolster soybean resilience against Al toxicity by enriching soil nutrients, activating enzymes, and bolstering antioxidant mechanisms. We also observed that it enriched root endophytic microbial diversity, notably increasing populations of <em>Nakamurella</em>, <em>Aureimonas</em>, <em>Luteimonas</em>, and <em>Sphingomonas</em>. These changes in the endophytes contributed to the improved adaptability of plants to adversity under Al toxicity. This study highlighted the potential of using ZnO NPs and BC as a sustainable approach to combat Al toxicity, emphasizing the intricate interplay between plant physiology and rhizosphere microbial dynamics in mitigating the effects of environmental toxicity.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109275"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Grapevine and maize: Two guard cell shaped strategies to cope with repeated drought stress","authors":"Patrick Pascal Lehr ,&nbsp;Alexander Erban ,&nbsp;Roman Paul Hartwig ,&nbsp;Monika Andrea Wimmer ,&nbsp;Joachim Kopka ,&nbsp;Christian Zörb","doi":"10.1016/j.plaphy.2024.109262","DOIUrl":"10.1016/j.plaphy.2024.109262","url":null,"abstract":"<div><div>Adaptation of crops to recurrent drought stress is crucial for maintaining agricultural productivity and achieving food security under changing climate. Guard cells, pivotal regulators of plant water usage and assimilation, are central to this adaptation process. However, the metabolic dynamics of guard cells under drought stress remain poorly understood, particularly in grapevine, a prominent crop grown in arid regions, and maize, a staple crop with substantial water requirements. In this study, differences in guard cells metabolism during drought stress of grapevine and maize were investigated by performing physiological and metabolomic analyses. Metabolomic analysis highlighted differential responses in amino acids and sugars, with grapevine guard cells displaying greater stability in amino acid and sugar signatures, while maize showed marked increases in sugar levels. These findings suggest two distinct adaptive strategies, a vigorous acclimation of guard cells, as observed in maize, and an attenuated acclimation of guard cells, shown in grapevine. Understanding these metabolic adjustments is helpful for enhancing drought resilience in agricultural systems.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109262"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The changes in sugar content and the selection of key genes at different developmental stages of 'Katy' and 'Kuijin' apricots","authors":"Xueping Han ,&nbsp;Caina Jiang ,&nbsp;GuipingWang ,&nbsp;Jinzheng Wang ,&nbsp;Peixian Nie ,&nbsp;Xiaomin Xue","doi":"10.1016/j.plaphy.2024.109280","DOIUrl":"10.1016/j.plaphy.2024.109280","url":null,"abstract":"<div><div>'Katy' and 'Kuijin' apricots are the main cultivated varieties in Shandong province. The flavor of the fruit is mainly determined by sugars and acids, with soluble sugar components serving as important nutritional elements in fruits as well as crucial indicators of fruit sweetness and flavor quality. However, little is known about the changes in soluble sugar content, especially sucrose content, and the sucrose metabolism mechanism during the entire fruit growth and development process of 'Katy' and 'Kuijin' apricots. In this study, we first detected the changes in sucrose, fructose, and glucose content at nine fruit development stages of 'Katy' and 'Kuijin' apricots, and found that the stage of rapid accumulation of sucrose and fructose was from 56 days after full bloom (DAF) to 63 DAF. Therefore, we identified the key gene <em>PaSS1</em> of sucrose synthase through transcriptome data screening, and further analyzed the function of the <em>PaSS1</em> gene in fruit sucrose metabolism process using virus-induced gene silencing (VIGS) technology. Silencing the <em>PaSS1</em> gene reduced the breakdown activity of sucrose synthase, increasing sucrose content while decreasing glucose and fructose content, delaying fruit coloring and ripening, indicating that the <em>PaSS1</em> gene may regulate the ripening of apricot fruits. This study provides a theoretical basis for further research on the molecular mechanism of the <em>PaSS1</em> gene in apricot fruit ripening process.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109280"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics analysis reveals the involvement of OnDIVARICATA 3 in controlling dynamic flower coloring of Oncidium hybridum","authors":"Yuanhua Luo ,&nbsp;Yan Chen ,&nbsp;Nengyan Fang,&nbsp;Lan Kong,&nbsp;Rongyan Lin,&nbsp;Yiquan Chen,&nbsp;Ronghui Fan,&nbsp;Huaiqin Zhong,&nbsp;Minling Huang,&nbsp;Xiuxian Ye","doi":"10.1016/j.plaphy.2024.109277","DOIUrl":"10.1016/j.plaphy.2024.109277","url":null,"abstract":"<div><div>Flower color is one of the main quality and economic traits of ornamental plants, and a large amount of research on flower color mainly focuses on the differences between varieties, while there were few reports on the change of flower color at different developmental stages. In this study, the metabolome and transcriptome of a new strain ‘XM-1’ with dynamic color changes of <em>Oncidium</em> were analyzed. The results showed that rutin, quercetin and carotenoids metabolism decreased significantly during the change of color from yellow to white. Analyzing the correlation network between metabolites and differential expressed genes, 25 key structural genes were detected and regulated by multiple MYB-related transcription factors. The MYB-related transcription factor <em>Cluster-100966.1_OnDIVARICATA 3</em> was selected for further analysis. The phylogenetic tree of DIVARICATA in different species of Orchidaceae and <em>Arabidopsis thaliana</em> was constructed and the most closely related members were selected for sequence comparison. The results showed that OnDIVARICATA 3 contained MYB-like conserved domains. Subcellular localization results showed that OnDIVARICATA 3 was located in the nucleus. In overexpressing <em>OnDIVARICATA 3</em> transgenic hairy roots, the expression of flower color related genes <em>FLS</em>, <em>ZEP</em>, and <em>CHYB</em> were significantly up-regulated. In summary, this study characterized the key metabolic pathways in the formation of the dynamic flower color of <em>Oncidium hybridum</em>, and constructed the regulatory network of the MYB-related. These results laid a theoretical foundation for the subsequent research on flower color and genetic engineering technology breeding of <em>Oncidium hybridum</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109277"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}