Plant Physiology and Biochemistry最新文献

{"title":"Ascorbic acid on the induction of salt stress tolerance in guava in the seedling formation phase.","authors":"Julio Cesar Agostinho da Silva, Jackson Silva Nóbrega, Geovani Soares de Lima, Lauriane Almeida Dos Anjos Soares, Jean Telvio Andrade Ferreira, Daniel da Conceição Almeida, Gleisson Dos Santos da Silva, Josélio Dos Santos da Silva, Cassiano Nogueira de Lacerda, Saulo Soares da Silva, André Alysson Rodrigues da Silva, Hans Raj Gehyi, Maila Vieira Dantas","doi":"10.1016/j.plaphy.2025.109506","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109506","url":null,"abstract":"<p><p>Guava is a fruit crop widely exploited in the Northeast region of Brazil. However, its exploitation is limited by water scarcity and, in many cases, producers are forced to use water with high levels of salts in irrigation. Thus, it is necessary to develop techniques to induce plant tolerance to salt stress, and the foliar application of a non-enzymatic compound such as ascorbic acid is a promising alternative to mitigate the deleterious effects on plants. The objective was to evaluate the effects of foliar application of ascorbic acid on guava plants, irrigated with saline waters in the seedling formation phase in a semi-arid region. The experiment was carried out in a greenhouse adopting a randomized block design, in a 5 × 4 factorial scheme, with five levels of electrical conductivity of water - ECw (0.3, 1.3, 2.3, 3.3 and 4.3 dS m^-1) and four concentrations of ascorbic acid - AsA (0, 200, 400 and 600 mg L^-1) with four replicates. Water salinity from 0.57 dS m^-1 compromised the gas exchange and biosynthesis of photosynthetic pigments, besides inhibiting the growth and quality of guava seedlings cv. Paluma. The concentration of 375 mg L^-1 AsA increases the production of photosynthetic pigments under low salinity conditions (0.3 dS m^-1) and increases the number of leaves and dry matter of seedlings up to 1.55 dS m^-1. Foliar application of up to 450 mg L^-1 attenuated the deleterious effects of salt stress on gas exchange and growth of guava seedlings. Guava cv. Paluma seedlings present higher quality when produced with an ECw of 0.55 dS m^-1 and under a concentration of 75 mg L^-1 of AsA at 150 days after sowing.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109506"},"PeriodicalIF":6.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological, physiological and transcriptional analyses provide insights into the biosynthesis of phenolics in Juniperus rigida under UV-B treatment.","authors":"Xueping Feng, Dongmei Wang, Dengwu Li","doi":"10.1016/j.plaphy.2025.109534","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109534","url":null,"abstract":"<p><p>Phenolics play a crucial role in plant defense mechanisms against increased UV-B radiation. Due to their significant medicinal properties, the phenolic compounds produced by Juniperus rigida have great potential as valuable sources for medicine. However, the process of synthesizing J. rigida phenolics under UV-B treatment remained unclear. To investigate the mechanism of J. rigida phenolic synthesis, this study examined the pattern of phenolics in J. rigida by analyzing its morphological structure, physiology, and key enzyme genes. The results showed that the phenolic compounds in J. rigida increased under low-intensity UV-B treatment, whereas a decrease was observed under high-intensity UV-B treatment. In particular, rutin and quercitrin were identified as key contributors to the antioxidant activity under UV-B treatment. Phenolics were mainly found in living cells of J. rigida needles, such as the vesicle-containing sheath cells, secretory cells, palisade tissue, spongy tissue, epidermis, endodermis, and parenchyma. Furthermore, the cell nucleus was significantly larger and darker after UV-B treatment. There were also temporal differences between the gene expression and accumulation of bioactive compound. Notably, 4CL, CHS, CHI, FLS, and PLR are the key genes for phenolics synthesis among the differentially expressed genes. These findings would not only elucidate the biosynthetic mechanism of phenolic compounds in J. rigida, but also provide a scientific basis for the use of UV-B radiation to synthesize active compounds in medicinal plants.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109534"},"PeriodicalIF":6.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of nitrate enhanced tobacco resistance to aphid infestation.","authors":"Changjian Miao, Zhi Hu, Xintong Liu, Huijing Ye, Hongzhen Jiang, Jinfang Tan, Jingguang Chen","doi":"10.1016/j.plaphy.2025.109514","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109514","url":null,"abstract":"<p><p>Tobacco is an economic crop that primarily relies on nitrate (NO₃^-) as its nitrogen source, and tobacco aphid is one of the significant pests that harm its growth. However, the impact of NO₃^- supply on the resistance of tobacco to aphids remains unclear. Present study investigated the effects of different NO₃^- concentrations supply on the reproductive capacity of tobacco aphids, impact of aphid infestation on tobacco growth, secondary metabolic and transcription changes. Physiological experiments were performed to verity the transcription analysis. The results indicated that aphids preferred tobacco treated with higher concentration of nitrate, showing greater reproductive capacity under high nitrate supply. From the results of transcriptome analysis, it can be seen that the gene expression of the shoot changed significantly after aphid and NO₃^- treatment. GO analysis showed that the pathways associated with cell wall biosynthesis were enriched in different groups. At the same time, RNA-seq analysis revealed several genes related to the pathway of aphid damage in tobacco, as well as some transcription factors associated with insect resistance. Inoculating tobacco with aphids under different NO₃^- concentration increased the levels of soluble sugars, free amino acids, jasmonic acid, and salicylic acid in shoot of tobacco. Additionally, it was observed that the cell wall development of leaves from low NO₃^- supply was incomplete, and the cell wall from high NO₃^- supply concentration is notably thicker. The lignin content was lower under lower NO₃^- supply, regardless of aphid inoculation. The trends of transcription levels in genes related to cell wall and lignin biosynthesis were consistent with the lignin contents. Collectively, our findings not only shed light on the physiological and biochemical responses of tobacco plants to NO₃^- treatment, but also offer novel perspectives for optimizing tobacco cultivation practices and enhancing insect resistance.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109514"},"PeriodicalIF":6.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of heat stress on the development, physiological and biochemical characteristics of Tartary buckwheat flowers, and its transcriptomic analysis.","authors":"Lisong Liu, Li Li, Yanjun Feng, Tao Wang, Chenglei Li, Huala Wu, Yufeng Hu, Qi Wu, Haixia Zhao","doi":"10.1016/j.plaphy.2025.109535","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109535","url":null,"abstract":"<p><p>Tartary buckwheat (Fagopyrum tataricum), a functional grain known for its medicinal and nutritional properties, has garnered significant attention due to its high flavonoid content and unique health benefits. Heat stress during the flowering stage can lead to sterility in Tartary buckwheat, resulting in reduced yields. This study investigates the effects of a treatment (30/27 °C for 7 days) on flower development, fertility, stress physiology, and gene expression in Tartary buckwheat, while also validating the efficacy of hormone treatments in alleviating the negative effects of heat stress. The results show that fertilization in Tartary buckwheat typically occurs within 3-5 days post-anthesis. By the 5th day, the stamen length in the heat-treated group was reduced by 13.89% compared to the control, while pistil length increased by 35.44%. Heat stress delayed the pistil stigma's transition into its highly receptive phase and caused a significant reduction in pollen viability by 15.25% after 5 days of treatment. Furthermore, after 7 days of treatment, the levels of H₂O₂ and O₂^- increased by 44.9% and 37.2%, respectively. However, Tartary buckwheat mitigated the impact of oxidative damage by enhancing the enzymatic activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Transcriptome analysis revealed that heat stress significantly suppressed the expression of genes in hormone signaling pathways, such as indole-3-acetic acid (IAA), gibberellin (GA), and jasmonic acid (JA). Under heat treatment conditions, exogenous hormone application significantly regulated the dynamics of flower development. Specifically, after 3 days of heat treatment, all hormone treatments significantly inhibited the abnormal elongation of stamens, with GA notably suppressing the abnormal elongation of pistils. After 5 days, GA significantly promoted stamen elongation, while IAA and jasmonic acid (JA) significantly inhibited the abnormal elongation of pistils. After 7 days, all three hormone treatments significantly promoted stamen elongation and effectively inhibited abnormal pistil growth. These results suggest that under heat stress conditions, GA plays a key role in promoting stamen elongation, while IAA and JA inhibit the abnormal elongation of the pistil. Prolonged high temperatures can impair the function of floral organs, while JA treatment on the seventh day of heat treatment effectively restored pistil receptivity and significantly improved pollen vitality. In summary, this study provides in-depth insights into the mechanisms by which heat stress affects flower development in Tartary buckwheat, offering theoretical foundations and practical guidance for reducing the impact of heat stress on buckwheat yield.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109535"},"PeriodicalIF":6.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The basic helix-loop-helix transcription factor PpeUNE12 regulates peach ripening by promoting polyamine catabolism and anthocyanin synthesis.","authors":"Wei Wang, Yang Chen, Yabo Jiang, Guangcai Tang, Luyue Guo, Gaozheng Qiao, Shihao Liu, Bin Tan, Jun Cheng, Langlang Zhang, Xia Ye, Xiaobei Wang, Haipeng Zhang, Xianbo Zheng, Shiwen Zhang, Jiancan Feng","doi":"10.1016/j.plaphy.2025.109537","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109537","url":null,"abstract":"<p><p>The basic helix-loop-helix (bHLH) transcription factors (TFs) play important roles in various plant developmental and biological processes. However, the precise mechanisms by which bHLH TFs regulate fruit ripening warrant further investigation. Polyamine oxidase (PAO) is crucial for polyamine (PA) catabolism and plays crucial roles in fruit ripening. However, the regulatory mechanism of PAO gene expression during fruit ripening remains largely unexplored. In this study, we identified a peach bHLH TF, PpeUNE12, which directly binds to and activates the promoter of PpePAO1. Silencing PpeUNE12 substantially increased PA accumulation and delayed peach fruit ripening, while overexpressing PpeUNE12 decreased PA accumulation and accelerated peach fruit ripening. Additionally, anthocyanin content decreased in PpeUNE12-silenced fruits but increased in PpeUNE12-overexpressing peach fruits compared to the control. RNA-seq and RT-qPCR analyses revealed that the majority of genes involved in anthocyanin biosynthesis, including PpeF3H, PpeCHS, PpeDFR, PpeUFGT and PpeMYB10.1 exhibited down-regulation in fruits with silenced PpeUNE12, while these genes were up-regulated in fruits overexpressing PpeUNE12. Although PpeUNE12 exhibited no direct binding to the promoters of PpeUFGT and PpeMYB10.1, it substantially activated their activity. This investigation is the first to provide evidence that bHLH regulates fruit maturation via promoting both PA catabolism and anthocyanin synthesis. It reveals a novel mechanism of bHLH in regulating fruit ripening and enhances our comprehension of the regulatory mechanism of PA catabolism and anthocyanin synthesis during fruit maturation.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109537"},"PeriodicalIF":6.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143040859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alginate oligosaccharide induces resistance against Penicillium expansum in pears by priming defense responses.","authors":"Solairaj Dhanasekaran, Lisha Liang, Yaqi Chen, Jingwei Chen, Shuaiying Guo, Xiaoyun Zhang, Lina Zhao, Hongyin Zhang","doi":"10.1016/j.plaphy.2025.109531","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109531","url":null,"abstract":"<p><p>The research intended to explore the control ability of alginate oligosaccharide (AOS) on Penicillium expansum infection in pear fruit by priming response and its mechanism. The results showed that 100 mg L^-1 AOS treatment could significantly reduce the incidence of postharvest blue mold and the lesion diameter in pear fruits and maintain their quality. The defense responses induced by AOS treatment alone were relatively mild in pear fruits. Still, AOS-treated pear fruits inoculated with P. expansum showed more intense disease resistance responses. These defense responses included enhanced activities of chitinase (CHI), β-1, 3-glucanase (GLU), peroxidase (POD), polyphenol oxidase (PPO), phenylalanine ammonia-lyase activity (PAL), along with the accumulation of total phenolic compounds, flavonoids and lignin. Additionally, the expression levels of defense-related genes, such as PbGLU, PbCHI, PbPAL, PbPOD and PbPPO, were significantly upregulated. However, AOS did not show a potential inhibitory effect on the in vitro growth of P. expansum. Our results indicated that AOS treatment in the postharvest pear fruit enhances disease resistance by priming its defense responses.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109531"},"PeriodicalIF":6.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An R2R3-MYB transcription factor PdbMYB6 enhances drought tolerance by mediating reactive oxygen species scavenging, osmotic balance, and stomatal opening.","authors":"Minglong Yan, Xinxin Li, Xiaoyu Ji, Biyao Gang, Ying Li, Zhuoran Li, Yucheng Wang, Huiyan Guo","doi":"10.1016/j.plaphy.2025.109536","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109536","url":null,"abstract":"<p><p>Drought is a major environmental challenge that hinders the growth and development of plants. R2R3-MYB transcription factors (TFs) play a vital role in mediating responses to abiotic stress; however, their specific functions in Populus davidiana × Populus bolleana hybrid poplar plants remain underexplored. This study focused on PdbMYB6, a novel R2R3-MYB TF identified in P. davidiana × P. bolleana plants. We found that PdbMYB6 acts as a transcriptional activator. By conducting functional analyses of both overexpression and knockout models, we demonstrated that PdbMYB6 enhances drought tolerance in plants by improving reactive oxygen species scavenging and modulating osmotic balance. Additionally, PdbMYB6 plays a role in regulating stomatal openings to minimize water loss. The qRT-PCR and RNA sequencing results revealed that PdbMYB6 influences the expression of genes related to stress tolerance. TF-centered Yeast One-Hybrid (Y1H) and chromatin immunoprecipitation (ChIP) assays indicated that PdbMYB6 binds to two novel core sequences (C [A/G/C]TG and [T/A/G]GTA) as well as GT-1 (GGAAA) and MYBCORE (AACGG) elements, which are associated with light responses and stress resistance, thereby promoting the expression of stress-resistant genes. Furthermore, Y1H and ChIP assays identified four upstream factors that regulate PdbMYB6 expression by interacting with specific elements in its promoter. Notably, the overexpression of these four factors enhances plant drought resistance and affects the expression of stress-response genes. Our findings highlight the role of the PdbMYB6 TF in the drought regulatory mechanism and provide potential gene sources for the molecular breeding of drought-resistant plants through genetic engineering.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109536"},"PeriodicalIF":6.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AhASRK1, a peanut dual-specificity kinase that activates the Ca²⁺-ROS-MAPK signalling cascade to mediate programmed cell death induced by aluminium toxicity via ABA.","authors":"Xue-Zhen Fu, Xin Wang, Jing-Jing Liu, Yu-Xi Chen, Ai-Qin Wang, Jie Zhan, Zhu-Qiang Han, Long-Fei He, Dong Xiao","doi":"10.1016/j.plaphy.2025.109538","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109538","url":null,"abstract":"<p><p>Aluminium (Al)-induced programmed cell death (PCD) is thought to be a main cause of Al phytotoxicity. However, the underlying mechanism by which Al induces PCD in plants is unclear. In this study, we characterized the function of AhASRK1 (Aluminum Sensitive Receptor-like protein Kinase1), an Al-induced LRR-type receptor-like kinase gene. AhASRK1 was localized on the plasma membrane. A kinase assay of recombinant cytoplasmic domains of AhASRK1 revealed that this leucine-rich repeat-receptor-like protein kinase autophosphorylates both serine/threonine and tyrosine residues. The role of AhASRK1 in regulating Al-induced PCD was investigated in roots. Al treatment significantly inhibited root growth and promoted ROS production and cell death after AhASRK1 was overexpressed in Arabidopsis, whereas the knockdown of AhASRK1 in peanut increased Al tolerance. AhASRK1 overexpression resulted in increased accumulation of apical calcium ions (Ca²⁺) and increased MAPK signalling under Al treatment; however, the AhASRK1-knockdown peanut lines exhibited a decrease in the Ca²⁺ concentration under Al stress. Furthermore, inhibition of ABA biosynthesis mitigated PCD occurrence and ROS accumulation under Al stress, as did Al-induced Ca²⁺ and p MAPK signalling. These results suggest that AhASRK1 mediates the occurrence of PCD through the ABA pathway to mediate the accumulation of Ca²⁺ and the production of ROS, thereby activating MAPK signalling. Additionally, AhASRK1 overexpression promoted leaf senescence and induced the transcription of a multitude of ABA-related genes. This study provides new clues for improving the phytotoxicity of Al in acidic soils.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109538"},"PeriodicalIF":6.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitric oxide mitigates cadmium stress by promoting the biosynthesis of cell walls in Robinia pseudoacacia roots.","authors":"Xun Wang, Shufeng Wang, Lan Gao, Pan Guo, Hongxia Du, Ming Ma, Heinz Rennenberg","doi":"10.1016/j.plaphy.2025.109544","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109544","url":null,"abstract":"<p><p>Cadmium (Cd) pollution is a growing concern worldwide, because it threatens human health through the food chain. Woody plants, such as the pioneer species black locust (Robinia pseudoacacia L.), are widely used in phytoremediation of Cd-contaminated soils, but strongly differ in Cd tolerance. Nitric oxide (NO), a highly reactive gas of biogenic and anthropogenic origin, has been shown to protect plants to Cd exposure. We investigated the protective mechanism of NO against Cd toxicity in black locust using physiological, transcriptomic and metabolomic approaches. We studied the correlation between cell wall traits, genes, and metabolites. The findings indicated that NO improved the growth of black locust under Cd exposure and elevated the fraction of Cd in the cell wall. NO increased cell wall thickness by stimulating the biosynthesis of pectin, cellulose, hemicellulose, and lignin. Transcriptomic and metabolomic analyses demonstrated that NO upregulated genes related to root cell wall biosynthesis and increased the accumulation of related metabolites, thereby increasing the Cd resistance of black locust. Our results elucidated a molecular mechanism underlying NO-mediated Cd tolerance in black locust and provided novel insights for phytoremediation of Cd-polluted soils by woody plants.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109544"},"PeriodicalIF":6.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus subtilis inoculated in organic compost could improve the root architecture and physiology of soybean under water deficit.","authors":"Beatriz V Moraes, Milene I S Coelho, Patrick S Silva, Ademir S F Araujo, Aurenivia Bonifacio, Arthur P A Pereira, Erika V de Medeiros, Fabio F Araujo","doi":"10.1016/j.plaphy.2025.109540","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109540","url":null,"abstract":"<p><p>Bacillus subtilis is known to promote root growth and improve plant physiology, while organic compost enhances soil water retention. This study explored the combined effect of inoculating B. subtilis in organic compost on soybean growth under water deficit. The treatments included chemical fertilization, non-inoculated organic compost, and organic compost inoculated with B. subtilis which were assessed under well-watered and water-deficit conditions. The organic compost inoculated with B. subtilis increased root biomass, length, volume, and the number of root tips under well-watered conditions, although it reduced root diameter. Under water deficit, the organic compost inoculated with B. subtilis increased root tip number (∼150%), biomass (∼95%) and number (∼85%) of nodules. Water deficit negatively affected soybean physiology, reduced photosynthesis, transpiration, and stomatal conductance, while increased internal CO₂ concentration. However, the organic compost inoculated with B. subtilis mitigated these effects, enhancing photosynthesis (∼20%) and water use efficiency (∼25%). Under water deficit, this treatment also increased shoot biomass by 15% and the drought tolerance index by 51% compared to the control. The combination of B. subtilis and organic compost improved root architecture, nodulation, and drought tolerance. These results suggest that B. subtilis inoculated in the organic compost is a promising strategy for enhancing soybean productivity and resilience under water stress, offering a novel approach to mitigating drought effects in agriculture.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109540"},"PeriodicalIF":6.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}