Rice specific novel miRNA, Osa-miR39 targets 9-cis-epoxycarotenoid dioxygenases (NCEDs) to regulate fluoride stress response

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-10-02 DOI:10.1016/j.plaphy.2025.110582

Tamarapalli Sravya Sruti, Sasmita Mohanty, Raj Kumar Joshi

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引用次数: 0

Abstract

Fluoride contamination in rice poses a serious risk to human health. MicroRNAs (miRNAs) regulate gene expression under stress conditions, yet their role in fluoride stress remains unclear. Comparative profiling of fluoride-sensitive (IR-64) and tolerant (Gobindobhog, GB) cultivars identified 436 fluoride-responsive miRNAs, of which 39 (30 conserved and 9 novel) were differentially expressed. Target prediction and expression analyses revealed regulation of stress-associated transcription factors, with inverse correlations between several miRNAs and their targets. A novel miRNA, Osa-miR39, was strongly induced in GB but suppressed in IR-64 under fluoride stress. The expression levels of its three abscisic acid (ABA)-responsive target genes encoding 9-cis-epoxycarotenoid dioxygenases (NCED3 & NCED5) and aldehyde oxidase (AO) were suppressed in GB, suggesting a potential role in fluoride tolerance. The regulatory interaction between Osa-miR39 and its targets was further validated through transient co-expression assays in Nicotiana benthamiana. Moreover, the overexpression of Osa-miR39 in the sensitive rice cultivar IR-64 conferred enhanced fluoride tolerance concomitant with repression of OsNCED genes. Collectively, these findings identify Osa-miR39 as a key regulator of fluoride stress tolerance in rice.

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水稻特异性miRNA Osa-miR39靶向9-顺式环氧类胡萝卜素双加氧酶（NCEDs）调控氟胁迫反应

大米中的氟化物污染对人类健康构成严重威胁。MicroRNAs （miRNAs）调节应激条件下的基因表达，但它们在氟胁迫中的作用尚不清楚。对氟敏感（IR-64）和耐氟品种（Gobindobhog， GB）进行比较分析，鉴定出436个氟敏感mirna，其中39个（30个保守mirna和9个新mirna）差异表达。靶标预测和表达分析揭示了应激相关转录因子的调控，几种mirna与其靶标之间存在负相关关系。在氟胁迫下，一种新的miRNA Osa-miR39在GB中被强烈诱导，而在IR-64中被抑制。其编码9-顺式环氧类胡萝卜素双加氧酶（NCED3 & NCED5）和醛氧化酶（AO）的3个ABA应答靶基因在GB中表达水平被抑制，提示其在氟耐受中可能起作用。Osa-miR39与其靶点之间的调控相互作用通过在烟叶中的瞬时共表达实验进一步得到验证。此外，在敏感的水稻品种IR-64中，Osa-miR39的过表达增强了氟耐受性，同时抑制了OsNCED基因。总之，这些发现确定Osa-miR39是水稻氟胁迫耐受性的关键调节因子。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.