CRISPR/Cas9-targeted mutagenesis of the PpTCP4 gene increased tiller number in Poa pratensis

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-10-02 DOI:10.1016/j.plaphy.2025.110588

Cheng Li , Chen Meng , Xinzhi Wang , Chao Yuan , Qian Chen , Ya Yang , Zhengshe Zhang , Yuanwen Duan , Quanmin Dong , Xudong Sun

引用次数: 0

Abstract

Poa pratensis, commonly known as Kentucky bluegrass, is a widely used cool-season grass species for turf in lawns and recreational areas worldwide. However, the lack of genome editing platforms has impeded research into enhancing forage crops suitable for land unsuitable for other crops. In this study, we successfully established an Agrobacterium-mediated genetic transformation system for P. pratensis. Furthermore, we successfully produced PpTCP4-edited plants using the CRISPR/Cas9 system. This research represents a significant advancement in the genetic transformation and gene editing of P. pratensis, providing a foundational technology for future investigations into gene function and breeding in P. pratensis.

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以CRISPR/ cas9为靶点诱变PpTCP4基因，增加了草芥的分蘖数。

Poa pratensis，俗称肯塔基蓝草，是一种广泛使用的冷季草品种，在世界各地的草坪和娱乐场所的草坪。然而，缺乏基因组编辑平台阻碍了对适合种植其他作物的土地的饲料作物进行改良的研究。在这项研究中，我们成功地建立了一个农杆菌介导的草地草遗传转化体系。此外，我们使用CRISPR/Cas9系统成功地生产了pptcp4编辑的植物。本研究在草地草地的遗传转化和基因编辑方面取得了重大进展，为今后草地草地草地基因功能和育种研究提供了基础技术。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.