Functional characterization of TaNAC6-3B: A key regulator of drought tolerance in wheat (Triticum aestivum L.)

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-09-30 DOI:10.1016/j.plaphy.2025.110578

Nan Chen , Xiang Li , Yong-jia Feng , De-jun Han , Wei-jun Zheng , Zhen-sheng Kang

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引用次数: 0

Abstract

Drought stress is a major abiotic constraint limiting wheat (Triticum aestivum L.) productivity. Previous studies have shown that reducing irrigation water use by approximately 40 % can cause a 20.6 % decrease in wheat yield. Therefore, improving drought resistance is a priority in wheat breeding programs. For genetic improvement of drought tolerance, systematic investigation of drought-responsive molecular mechanisms is crucial. In this study, comparative transcriptome analysis was conducted on leaf and root tissues of the drought-tolerant wheat cultivar ChangWu134 under well-watered and drought-stressed conditions. Further systematic analysis identified a key drought tolerance gene TaNAC6-3B. Functional characterization of the candidate NAC (NAM, ATAF1/2, and CUC2) family transcription factor TaNAC6-3B revealed its nuclear localization. Transgenic overexpression lines had significantly enhanced drought tolerance, and transcriptome profiling revealed up-regulation of NCED and ABA responsive genes and drought-responsive genes. Mechanism studies have revealed that TaNAC6-3B activates the expression of the LEA (Late embryogenesis abundant) protein gene TaLEA1-2B via direct binding to its promoter. The results of this study provide clues for analysis of the genetic basis of drought tolerance in ChangWu134, and also provide candidate genetic resources for breeding for drought tolerance.

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小麦耐旱性关键调控因子TaNAC6-3B的功能研究

干旱胁迫是制约小麦产量的主要非生物胁迫。先前的研究表明，减少约40%的灌溉用水量可导致小麦产量下降20.6%。因此，提高抗旱性是小麦育种计划的重点。对抗旱分子机制的系统研究是抗旱遗传改良的关键。本研究对抗旱小麦品种长武134在水分充足和干旱胁迫条件下的叶片和根系组织进行了比较转录组分析。进一步的系统分析确定了关键的耐旱基因TaNAC6-3B。候选NAC （NAM、ATAF1/2和CUC2）家族转录因子TaNAC6-3B的功能表征揭示了其核定位。转基因过表达系的耐旱性显著增强，转录组分析显示NCED和ABA响应基因以及干旱响应基因上调。机制研究表明，TaNAC6-3B通过直接结合启动子激活LEA （Late embryogenesis abundant）蛋白基因TaLEA1-2B的表达。本研究结果为分析长武134抗旱遗传基础提供了线索，也为抗旱育种提供了候选遗传资源。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.