Plant Physiology and Biochemistry最新文献_第3页

Genome-wide characterization of the DIR gene family in sesame reveals the function of SiDIR21 in lignan biosynthesis 芝麻中 DIR 基因家族的全基因组特征揭示了 SiDIR21 在木质素生物合成中的功能。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-08 DOI: 10.1016/j.plaphy.2024.109282

Huan Li , Fengduo Hu , Jianglong Zhou, Li Yang, Donghua Li, Rong Zhou, Ting Zhou, Yanxin Zhang, Linhai Wang, Jun You

{"title":"Genome-wide characterization of the DIR gene family in sesame reveals the function of SiDIR21 in lignan biosynthesis","authors":"Huan Li , Fengduo Hu , Jianglong Zhou, Li Yang, Donghua Li, Rong Zhou, Ting Zhou, Yanxin Zhang, Linhai Wang, Jun You","doi":"10.1016/j.plaphy.2024.109282","DOIUrl":"10.1016/j.plaphy.2024.109282","url":null,"abstract":"<div><div>Furofuran-type lignans, mainly sesamin and sesamolin, are the most representative functional active ingredients in sesame (<em>Sesamum indicum</em> L.). Their exceptional antioxidant properties, medicinal benefits, and health-promoting functions have garnered significant attention. Dirigent (DIR) proteins, found in vascular plants, are crucial for the biosynthesis of secondary metabolites, like lignans, and essential for responding to abiotic and biotic stresses. Despite their importance, they have yet to be systematically analyzed, especially those involved in lignan synthesis in sesame. This study unveiled 44 DIR genes in sesame. Phylogenetic analysis categorized these SiDIRs into five subgroups (DIR-a, DIR-b/d, DIR-e, DIR-f, and DIR-g), aligning with conserved motifs and gene structures analyses. Expression analysis unveiled distinct tissue-specific and hormone-responsive expression patterns among the SiDIR gene family members. Particularly, <em>SiDIR21</em>, a member of the DIR-a subgroup, exhibited robust expression in lignan-accumulating tissues and consistently high expression levels in germplasm during seed development with high sesamin content. Furthermore, <em>SiDIR21</em> overexpression in hairy roots significantly increased sesamin and sesamolin contents, confirming its role in lignan synthesis. Overall, our study offers a valuable resource for exploring SiDIRs’ functions and the lignan biosynthesis pathway in sesame.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109282"},"PeriodicalIF":6.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histone demethylase JMJ713 interaction with JMJ708 modulating H3K36me2, enhances rice heat tolerance through promoting hydrogen peroxide scavenging 组蛋白去甲基化酶 JMJ713 与调节 H3K36me2 的 JMJ708 相互作用，通过促进过氧化氢清除增强水稻的耐热性。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-08 DOI: 10.1016/j.plaphy.2024.109284

Jiaxin Chai , Xiangyang Gu , Pengyu Song , Xinzhou Zhao , Yingjie Gao , Haiqi Wang , Qian Zhang , Tingting Cai , Yutong Liu , Xiaoting Li , Tao Song , Zhengge Zhu

{"title":"Histone demethylase JMJ713 interaction with JMJ708 modulating H3K36me2, enhances rice heat tolerance through promoting hydrogen peroxide scavenging","authors":"Jiaxin Chai , Xiangyang Gu , Pengyu Song , Xinzhou Zhao , Yingjie Gao , Haiqi Wang , Qian Zhang , Tingting Cai , Yutong Liu , Xiaoting Li , Tao Song , Zhengge Zhu","doi":"10.1016/j.plaphy.2024.109284","DOIUrl":"10.1016/j.plaphy.2024.109284","url":null,"abstract":"<div><div>The Earth is currently undergoing rapid warming cause of the accumulation in greenhouse gas emissions into the atmosphere and the consequent rise in global temperatures. High temperatures can bring the effects on rice development and growth and thereby decrease rice yield. In this study, we have identified that both JMJ713 and JMJ708 possess distinct histone demethylase activities. Specifically, JMJ713 modulates the levels of H3K36me2 while JMJ708 alters H3K9me3. Additionally, we have observed an interaction between JMJ713 and JMJ708, which collectively modify the level of H3K36me2. Furthermore, our findings demonstrate that JMJ713 plays an essential role to heat stress responses in rice (<em>Oryza sativa</em>). The overexpression of <em>JMJ713</em> enhances heat tolerance in rice, whereas <em>JMJ7</em>13 RNA interference rice lines exhibit increased sensitivity to heat. Further investigations revealed that overexpression of <em>JMJ713</em> activated catalase (CAT) and peroxidase (POD) activities by mitigating excessive accumulation of reactive oxygen species (ROS) caused by heat stress. Interestingly, the setting rates of <em>JMJ7</em>13 RNA interference lines decreased in comparing to wild-type, indicating that <em>JMJ7</em>13 might play a crucial role in the rice seed development stage as well. Collectively, this study not only highlights JMJ713 is involved in heat stress responses but also provides insights into the conserved Fe(Ⅱ) and α-ketoglutarate (KG) binding residues are crucial for the demethylase activity of JMJ713, as well as JMJ713 interacts with JMJ708 to jointly regulate the levels of H3K36me2.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109284"},"PeriodicalIF":6.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZmDnaJ-ZmNCED6 module positively regulates drought tolerance via modulating stomatal closure in maize. ZmDnaJ-ZmNCED6 模块通过调节玉米气孔关闭正向调节耐旱性。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-08 DOI: 10.1016/j.plaphy.2024.109286

Anyi Dong, Nan Wang, Tinashe Zenda, Xiuzhen Zhai, Yuan Zhong, Qian Yang, Yue Xing, Huijun Duan, Xiaocui Yan

{"title":"ZmDnaJ-ZmNCED6 module positively regulates drought tolerance via modulating stomatal closure in maize.","authors":"Anyi Dong, Nan Wang, Tinashe Zenda, Xiuzhen Zhai, Yuan Zhong, Qian Yang, Yue Xing, Huijun Duan, Xiaocui Yan","doi":"10.1016/j.plaphy.2024.109286","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109286","url":null,"abstract":"<p><p>Heat Shock Protein plays a vital role in maintaining protein homeostasis and protecting cells from stress stimulation. As one of the HSP40 proteins, DnaJ is a stress response protein widely existing in plant cells. The function and regulatory mechanism of ZmDnaJ, a novel chloroplast-localized type-III HSP40, in maize drought tolerance were characterized. Tissue-specific expression analysis showed that ZmDnaJ is highly expressed in the leaves, and is strongly drought-induced in maize seedlings. Overexpression of ZmDnaJ improved maize drought tolerance by enhancing stomatal closure and increasing ABA content to mediate photosynthesis. In contrast, the CRISPR-Cas9 knockout zmdnaj mutant showed lower relative water content and high sensitivity to drought stress. Moreover, Y2H, BiFC and Co-IP analyses revealed that ZmDnaJ interacts with an ABA synthesis-related protein ZmNCED6 to regulate drought tolerance. Similarly, ZmNCED6 overexpressed lines showed stronger oxidation resistance, enhanced photosynthetic rate, stomatal closure and ABA content, whilst the CRISPR-Cas9 knockout mutant showed sensitive to drought stress. More importantly, ZmDnaJ could regulate key drought tolerance genes (ZmPYL10, ZmPP2C44, ZmEREB65, ZmNCED4, ZmNCED6 and ZmABI5), involved in ABA signal transduction pathways. Taken together, our findings suggest that ZmDnaJ-ZmNCED6 module improves drought tolerance in maize.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"218 ","pages":"109286"},"PeriodicalIF":6.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas putida triggers phosphorus bioavailability and P-transporters under different phosphate regimes to enhance maize growth 假单胞菌（Pseudomonas putida）在不同磷酸盐制度下触发磷生物利用率和磷转运体，以促进玉米生长。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-07 DOI: 10.1016/j.plaphy.2024.109279

Tanya Singh , Nikita Bisht , Mohd Mogees Ansari , Puneet Singh Chauhan

{"title":"Pseudomonas putida triggers phosphorus bioavailability and P-transporters under different phosphate regimes to enhance maize growth","authors":"Tanya Singh , Nikita Bisht , Mohd Mogees Ansari , Puneet Singh Chauhan","doi":"10.1016/j.plaphy.2024.109279","DOIUrl":"10.1016/j.plaphy.2024.109279","url":null,"abstract":"<div><div>The decline of available phosphorus in soil due to anthropogenic activities necessitates utilizing soil microorganisms that influence soil phosphorus levels. However, the specific mechanisms governing their interaction in <em>Zea mays</em> under diverse phosphate regimes remain largely unknown. The present study investigated the dynamics of phosphorus solubilization and the impact of organic acid supplementation in combination with the beneficial rhizobacterium <em>Pseudomonas putida</em> (RA) on maize growth under phosphorus-limiting and unavailable conditions. HPLC analysis revealed gluconic acid as the primary organic acid (OA) produced by <em>P. putida</em> across all three conditions (P-sufficient, P-limiting, and P-unavailable), with the highest production occurring under P-limiting conditions. The study evaluates the effects of RA, OA, and OA + RA on plant growth parameters under P-limiting and insufficient conditions, revealing significant alterations in growth and biochemical parameters (<em>P =</em> 0.05) compared to their respective untreated controls. Additionally, plants treated with organic acids and bacterial inoculation show increased phosphorus concentrations in both roots and shoots. Gene expression analysis of key phosphorus transporter genes (<em>PHT1, PHO1, PTF, PHF1</em>) further supports the role of organic acids and bacterial inoculation in enhancing phosphorus uptake. In conclusion, our study affirms that the secretion of gluconic acid by RA and its plant growth-promoting properties boost phosphorus uptake and maize growth by increasing phosphorus availability and influencing the expression of phosphorus transport-related genes.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109279"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the synergistic effects of nano-zinc and biochar in mitigating aluminum toxicity in soybeans 研究纳米锌和生物炭在减轻大豆铝毒性方面的协同效应。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-07 DOI: 10.1016/j.plaphy.2024.109275

Enxi Zhang , Kun Liu , Suwen Liang , Lingrui Liu , Hai Nian , Tengxiang Lian

{"title":"Investigating the synergistic effects of nano-zinc and biochar in mitigating aluminum toxicity in soybeans","authors":"Enxi Zhang , Kun Liu , Suwen Liang , Lingrui Liu , Hai Nian , Tengxiang Lian","doi":"10.1016/j.plaphy.2024.109275","DOIUrl":"10.1016/j.plaphy.2024.109275","url":null,"abstract":"<div><div>Aluminum (Al) toxicity limited root growth by reducing nutrient translocation and promoting reactive oxygen species (ROS) accumulation, particularly in soybean. The endophyte of root could be modified by plant metabolites, which could potentially alter the tolerance to environmental toxicity of plants in acidic-Al soils. To explore how they help soybean mitigate Al toxicity by altering root endophytes, zinc oxide nanoparticles (ZnO NPs) at doses of 0, 30, 60, 90 mg/kg and 2% biochar (BC) were selected as bio modifiers, and Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> at 19 mg/kg was used to simulate Al toxicity. We analyzed root endophytes and metabolites by high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS). We found that ZnO NPs with BC could bolster soybean resilience against Al toxicity by enriching soil nutrients, activating enzymes, and bolstering antioxidant mechanisms. We also observed that it enriched root endophytic microbial diversity, notably increasing populations of <em>Nakamurella</em>, <em>Aureimonas</em>, <em>Luteimonas</em>, and <em>Sphingomonas</em>. These changes in the endophytes contributed to the improved adaptability of plants to adversity under Al toxicity. This study highlighted the potential of using ZnO NPs and BC as a sustainable approach to combat Al toxicity, emphasizing the intricate interplay between plant physiology and rhizosphere microbial dynamics in mitigating the effects of environmental toxicity.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109275"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grapevine and maize: Two guard cell shaped strategies to cope with repeated drought stress 葡萄和玉米：应对反复干旱胁迫的两种护卫细胞形态策略

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-07 DOI: 10.1016/j.plaphy.2024.109262

Patrick Pascal Lehr , Alexander Erban , Roman Paul Hartwig , Monika Andrea Wimmer , Joachim Kopka , Christian Zörb

{"title":"Grapevine and maize: Two guard cell shaped strategies to cope with repeated drought stress","authors":"Patrick Pascal Lehr , Alexander Erban , Roman Paul Hartwig , Monika Andrea Wimmer , Joachim Kopka , Christian Zörb","doi":"10.1016/j.plaphy.2024.109262","DOIUrl":"10.1016/j.plaphy.2024.109262","url":null,"abstract":"<div><div>Adaptation of crops to recurrent drought stress is crucial for maintaining agricultural productivity and achieving food security under changing climate. Guard cells, pivotal regulators of plant water usage and assimilation, are central to this adaptation process. However, the metabolic dynamics of guard cells under drought stress remain poorly understood, particularly in grapevine, a prominent crop grown in arid regions, and maize, a staple crop with substantial water requirements. In this study, differences in guard cells metabolism during drought stress of grapevine and maize were investigated by performing physiological and metabolomic analyses. Metabolomic analysis highlighted differential responses in amino acids and sugars, with grapevine guard cells displaying greater stability in amino acid and sugar signatures, while maize showed marked increases in sugar levels. These findings suggest two distinct adaptive strategies, a vigorous acclimation of guard cells, as observed in maize, and an attenuated acclimation of guard cells, shown in grapevine. Understanding these metabolic adjustments is helpful for enhancing drought resilience in agricultural systems.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109262"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The changes in sugar content and the selection of key genes at different developmental stages of 'Katy' and 'Kuijin' apricots Katy "和 "Kuijin "杏不同发育阶段含糖量的变化和关键基因的选择。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-07 DOI: 10.1016/j.plaphy.2024.109280

Xueping Han , Caina Jiang , GuipingWang , Jinzheng Wang , Peixian Nie , Xiaomin Xue

{"title":"The changes in sugar content and the selection of key genes at different developmental stages of 'Katy' and 'Kuijin' apricots","authors":"Xueping Han , Caina Jiang , GuipingWang , Jinzheng Wang , Peixian Nie , Xiaomin Xue","doi":"10.1016/j.plaphy.2024.109280","DOIUrl":"10.1016/j.plaphy.2024.109280","url":null,"abstract":"<div><div>'Katy' and 'Kuijin' apricots are the main cultivated varieties in Shandong province. The flavor of the fruit is mainly determined by sugars and acids, with soluble sugar components serving as important nutritional elements in fruits as well as crucial indicators of fruit sweetness and flavor quality. However, little is known about the changes in soluble sugar content, especially sucrose content, and the sucrose metabolism mechanism during the entire fruit growth and development process of 'Katy' and 'Kuijin' apricots. In this study, we first detected the changes in sucrose, fructose, and glucose content at nine fruit development stages of 'Katy' and 'Kuijin' apricots, and found that the stage of rapid accumulation of sucrose and fructose was from 56 days after full bloom (DAF) to 63 DAF. Therefore, we identified the key gene <em>PaSS1</em> of sucrose synthase through transcriptome data screening, and further analyzed the function of the <em>PaSS1</em> gene in fruit sucrose metabolism process using virus-induced gene silencing (VIGS) technology. Silencing the <em>PaSS1</em> gene reduced the breakdown activity of sucrose synthase, increasing sucrose content while decreasing glucose and fructose content, delaying fruit coloring and ripening, indicating that the <em>PaSS1</em> gene may regulate the ripening of apricot fruits. This study provides a theoretical basis for further research on the molecular mechanism of the <em>PaSS1</em> gene in apricot fruit ripening process.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109280"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiomics analysis reveals the involvement of OnDIVARICATA 3 in controlling dynamic flower coloring of Oncidium hybridum 多组学分析揭示了 OnDIVARICATA 3 参与控制 Oncidium hybridum 的动态花色。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-07 DOI: 10.1016/j.plaphy.2024.109277

Yuanhua Luo , Yan Chen , Nengyan Fang, Lan Kong, Rongyan Lin, Yiquan Chen, Ronghui Fan, Huaiqin Zhong, Minling Huang, Xiuxian Ye

{"title":"Multiomics analysis reveals the involvement of OnDIVARICATA 3 in controlling dynamic flower coloring of Oncidium hybridum","authors":"Yuanhua Luo , Yan Chen , Nengyan Fang, Lan Kong, Rongyan Lin, Yiquan Chen, Ronghui Fan, Huaiqin Zhong, Minling Huang, Xiuxian Ye","doi":"10.1016/j.plaphy.2024.109277","DOIUrl":"10.1016/j.plaphy.2024.109277","url":null,"abstract":"<div><div>Flower color is one of the main quality and economic traits of ornamental plants, and a large amount of research on flower color mainly focuses on the differences between varieties, while there were few reports on the change of flower color at different developmental stages. In this study, the metabolome and transcriptome of a new strain ‘XM-1’ with dynamic color changes of <em>Oncidium</em> were analyzed. The results showed that rutin, quercetin and carotenoids metabolism decreased significantly during the change of color from yellow to white. Analyzing the correlation network between metabolites and differential expressed genes, 25 key structural genes were detected and regulated by multiple MYB-related transcription factors. The MYB-related transcription factor <em>Cluster-100966.1_OnDIVARICATA 3</em> was selected for further analysis. The phylogenetic tree of DIVARICATA in different species of Orchidaceae and <em>Arabidopsis thaliana</em> was constructed and the most closely related members were selected for sequence comparison. The results showed that OnDIVARICATA 3 contained MYB-like conserved domains. Subcellular localization results showed that OnDIVARICATA 3 was located in the nucleus. In overexpressing <em>OnDIVARICATA 3</em> transgenic hairy roots, the expression of flower color related genes <em>FLS</em>, <em>ZEP</em>, and <em>CHYB</em> were significantly up-regulated. In summary, this study characterized the key metabolic pathways in the formation of the dynamic flower color of <em>Oncidium hybridum</em>, and constructed the regulatory network of the MYB-related. These results laid a theoretical foundation for the subsequent research on flower color and genetic engineering technology breeding of <em>Oncidium hybridum</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109277"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The response of DNA methyltransferase and demethylase genes to abiotic stresses in tomato seedling 番茄幼苗 DNA 甲基转移酶和去甲基化酶基因对非生物胁迫的响应

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-06 DOI: 10.1016/j.plaphy.2024.109276

Xuejuan Pan, Zesheng Liu, Li Feng, Chunlei Wang, Chan Liu, Ailing Li, Kangding Yao, Weibiao Liao

{"title":"The response of DNA methyltransferase and demethylase genes to abiotic stresses in tomato seedling","authors":"Xuejuan Pan, Zesheng Liu, Li Feng, Chunlei Wang, Chan Liu, Ailing Li, Kangding Yao, Weibiao Liao","doi":"10.1016/j.plaphy.2024.109276","DOIUrl":"10.1016/j.plaphy.2024.109276","url":null,"abstract":"<div><div>DNA methylation plays an important role in regulating plant growth, development and gene expression. However, less is known about the response of DNA methyltransferase and demethylase genes to various stresses. In this study, the effects of abiotic stresses on DNA methylation gene expression patterns in tomato seedlings were investigated. Results showed that most tomato DNA methyltransferase and demethylase genes contained stress-related elements. The expression of <em>SlDML1</em> was significantly induced by cadmium (Cd) and sodium chloride (NaCl) stresses. <em>SlDML2</em> was more sensitive and reached its maximum value under polyethylene (PEG) stress at 24 h. The expression of <em>SlMET3L</em> was repressed to varying degrees under Cd, NaCl and PEG stresses at 48 h. However, 5-aza-2′-deoxycytidine (5-azadC) treatment decreased the Cd and PEG stress tolerance by down-regulating the expression of DNA methyltransferase except for the <em>SlMET3L</em>, and up-regulating the expression levels of <em>SlDML2</em>, <em>SlDML3</em> and <em>SlDML4</em>, cadmium transporters (<em>SlHMA5</em>, <em>SlCAX3</em>, and <em>SlACC3</em>) and osmoregulators (<em>SlDREB</em>, <em>SlLEA</em> and <em>SlHSP70</em>). Whereas 5-azadC treatment alleviated the salt stress through up-regulating DNA methyltransferase gene expression, and down-regulating the expression level of <em>SlDML1</em>, <em>SlDML3</em>, and <em>SlDML4</em>, <em>SlHKT1</em>, <em>SlNHX1</em>, and <em>SlSOS1</em>. Collectively, 5-azadC impaired Cd and PEG stress tolerance and enhanced salt stress tolerance by regulating the expression of methylation-related and stress-related genes in tomato seedlings. These results may provide useful information for further analysing function and evolution of DNA methylation methyltransferase and demethylase genes in tomato under stress conditions.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109276"},"PeriodicalIF":6.1,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison analysis of ABCG subfamily in bamboo and the potential function of PeABCG15 in monolignol transport 竹子中 ABCG 亚家族的比较分析以及 PeABCG15 在单木质素转运中的潜在功能。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-11-06 DOI: 10.1016/j.plaphy.2024.109278

Hui Li , Ziyang Li , Kebin Yang , Zeming Lin , Chenglei Zhu , Yan Liu , Zhimin Gao

{"title":"Comparison analysis of ABCG subfamily in bamboo and the potential function of PeABCG15 in monolignol transport","authors":"Hui Li , Ziyang Li , Kebin Yang , Zeming Lin , Chenglei Zhu , Yan Liu , Zhimin Gao","doi":"10.1016/j.plaphy.2024.109278","DOIUrl":"10.1016/j.plaphy.2024.109278","url":null,"abstract":"<div><div>Lignin is a principal component of secondary cell wall and plays vital roles in various biological processes. In this study, 68 and 42 members of ABC transporter G subfamily (ABCG) were identified in <em>Bambusa amplexicaulis</em> and <em>Olyra latifolia</em>, which were less than that of 77 in moso bamboo (<em>Phyllostachys edulis</em>). Collinearity analysis showed that <em>ABCG</em>s had undergone robust purifying selection with lower functional differentiation. These ABCGs were clustered into two clades of WBC and PDR. Notably, <em>PeABCG15</em> was highly expressed with the lignification of bamboo shoot. The WGCNA revealed that <em>PeABCG15</em> was co-expressed with eight MYB genes, among which PeMYB203 was able to activate <em>PeABCG15</em> validated by Y1H, DLR, and GUS assays. Furthermore, over-expressing <em>PeABCG15</em> significantly enhanced the content of lignin and the expression levels of monolignol biosynthetic genes in <em>Arabidopsis thaliana</em>, conferring improved tolerance to exogenous coniferyl alcohol. Collectively, our findings elucidated the prospective contribution of PeABCG15 to monolignol transport, providing insights into the lignin biosynthesis mechanism in bamboo.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109278"},"PeriodicalIF":6.1,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0