Plant Physiology and Biochemistry最新文献

{"title":"Comprehensive characterization of the bHLH transcription factor family in Curcuma wenyujin and functional elucidation of CwbHLH27 in jasmonate-regulated sesquiterpenoid biosynthesis.","authors":"Shiyi Wu, Kaer Lan, Qian Wang, Yi Su, Dengyu Li, Jing Ma, Tianyuan Hu, Xiaopu Yin, Qiuhui Wei","doi":"10.1016/j.plaphy.2025.109527","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109527","url":null,"abstract":"<p><p>Curcuma wenyujin is acknowledged as a crucial medicinal plant containing essential oils, primarily composed of sesquiterpenoids. While numerous sesquiterpenoids exhibit versatile physiological activities, their levels in C. wenyujin are generally low, particularly the pivotal anti-cancer component elemene. Our previous research demonstrated that basic helix-loop-helix (bHLH) is involved in modulating jasmonate-mediated sesquiterpenoid biosynthesis. In this study, a total of 106 CwbHLHs were identified and systematically analyzed. Under MeJA treatment, the expression levels of CwbHLH15, CwbHLH27, CwbHLH58, CwbHLH73, and CwbHLH89 were significantly upregulated, whereas CwbHLH81 was downregulated. Subsequently, CwbHLH27 was selected for further functional characterization. CwbHLH27 overexpression resulted in increased levels of β-elemene, γ-elemene, β-caryophyllene, and curzerene in C. wenyujin leaves. The expression levels of CwHMGS, CwHMGR, CwDXS, CwDXR, CwFPPS, and CwHDR, key enzyme genes in sesquiterpenoid biosynthesis, were upregulated in transgenic lines. Conversely, CwbHLH27 silencing resulted in the opposite effects. Further analysis revealed that CwbHLH27 activated the transcription of CwHMGS, CwHMGR, and CwDXS by directly binding to the E-box cis-elements within their promoters. Moreover, CwbHLH27 interacts with CwJAZ1/17, thereby executing JA signal transduction and regulating sesquiterpenoid biosynthesis in C. wenyujin. Finally, we elucidated the molecular mechanism by which the CwJAZs-CwbHLH27 regulatory module regulates sesquiterpenoid biosynthesis in response to JA signaling. Our research provides a molecular foundation for biotechnological-assisted breeding of varieties with enhanced active ingredient content.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109527"},"PeriodicalIF":6.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated physiological, transcriptomic and metabolomic analyses reveal ROS regulatory mechanisms in two castor bean varieties under alkaline stress.","authors":"Zhigang Cui, Fei Hao, Xuan Dong, Yan Gao, Bingyu Yao, Yunlong Wang, Yongyong Zhang, Guolin Lin","doi":"10.1016/j.plaphy.2025.109518","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109518","url":null,"abstract":"<p><p>Saline-alkaline stress has caused severe ecological and environmental problems. Castor bean is a potential alkali-tolerant plant, however, its reactive oxygen species (ROS) regulatory mechanisms under alkaline stress remain unclear. This study investigated the physiological, transcriptomic, and metabolomic characteristics of two varieties (ZB8, alkaline-sensitive; JX22, alkaline-resistant) under alkaline stress. Results showed that under alkaline stress, JX22's root length was 1.66-fold greater than ZB8's, while its superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities were 1.25-, 1.41-, and 1.29-fold higher than ZB8's, respectively. The levels of superoxide anion (O₂^-) and malondialdehyde (MDA) in JX22 were 0.2- and 0.68-fold of those in ZB8, respectively. Integrated transcriptomic and metabolomic analyses revealed that regarding ROS generation, alkaline stress promoted the upregulation of ACX1 and RBOHD genes in JX22, enabling more efficient ROS signal transduction and subsequent stress response regulation. In terms of ROS signal transduction, alkaline stress induced significant upregulation of protein kinase-encoding genes including CPK4, CPK9, and CPK10 in JX22, which cooperated with RBOHD to regulate ROS production. Concerning ROS scavenging, significant upregulation of SODA, CAT2, and PRXⅡB genes ensured a more efficient enzymatic ROS scavenging system in JX22 under alkaline stress. In contrast, ZB8 could only rely on less efficient non-enzymatic systems, such as carotenoid antioxidants, to mitigate oxidative damage, where genes like CCD7, CYP897B and metabolites including lutein and zeaxanthin played crucial roles. These findings elucidate the ROS response mechanisms of castor bean under alkaline stress, paving new ways for breeding alkaline-resistant varieties.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109518"},"PeriodicalIF":6.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition mechanism of Microcystis aeruginosa in coculture of Lemna and Azolla: Insights from non-targeted Metabonomics.","authors":"Xiaobin Yang, Yushan Lou, Linyuan Song, Di Zhang, Yuzi Song, Jingxuan Liang, Zikuo Liu, Ce Wang, Zhao Zhao","doi":"10.1016/j.plaphy.2025.109529","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109529","url":null,"abstract":"<p><p>Microcystis aeruginosa, a harmful alga in cyanobacterial blooms, damages aquatic ecosystems. Species diversity may control the blooms by increasing ecosystem stability and resource utilization. The growth and photosynthetic systems of M. aeruginosa were investigated using the water from monocultures of Lemna aequinoctialis and Azolla imbricata group, as well as their mixtures. The highest rate of inhibition (84%) of M. aeruginosa was observed in the water excretions from the mixture of the two species across the three experimental groups. Greater disruption of cell membranes and a more significant decrease in the maximum electron transfer rate and photochemical quantum yield of M. aeruginosa were observed under mixed conditions compared to the monoculture, indicating the increased disruption of their photosynthetic systems in the mixed group. Liquid chromatography-mass spectrometry identified 479 and 431 differential metabolites in the mixed group compared to monocultures of L. aequinoctialis group and A. imbricata, respectively. Dihydrocapsaicin and 13-hydroxy-9-methoxy-10-oxo-11-octadecenoic acid, previously known to participate in oxidative stress and induce the secretion of benzoic acid to disrupt the cell membrane, were found to be abundant in the mixed group compared to the monoculture groups of L. aequinoctialis and A. imbricata. Our results showed that a mixture of L. aequinoctialis and A. imbricata is a potential novel antialgal agent to inhibit harmful algae.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109529"},"PeriodicalIF":6.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143040935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytoplasmic G6PDs modulate callus formation in Arabidopsis root explants through regulation of very-long-chain fatty acids accumulation.","authors":"Junjie Li, Xiaofan Na, Chang Qi, Ruiqing Shi, Kaile Li, Jie Jin, Ziyu Liu, Meiyun Pu, Shengwang Wang, Hao Sun, Xiaomin Wang, Yurong Bi","doi":"10.1016/j.plaphy.2025.109526","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109526","url":null,"abstract":"<p><p>Glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme in the pentose phosphate pathway, impacts cancer cell proliferation and plant stress responses. However, its role in plant cell dedifferentiation and callus formation is not well understood. This study explores the function of cytoplasmic G6PD isoforms in Arabidopsis pericycle cell reprogramming into callus by employing a suite of mutant analyses, qRT-PCR, and GC-MS. Our findings demonstrate that g6pd5/6 double mutants exhibit enhanced callus formation compared to wild-type and single mutants, implicating cytoplasmic G6PDs as negative regulators of callus development. The double mutant showed reduced NADPH levels and increased expression of very-long-chain fatty acid (VLCFA) biosynthesis genes and the VLCFA-downstream gene Aberrant Lateral Root Formation 4 (ALF4) on callus-inducing medium (CIM). Notably, VLCFA concentrations were decreased in g6pd5/6 mutants, and supplementation of VLCFA reduced callus area. Additionally, callus formation in the alf4/g6pd5/6 triple mutant aligned with wild-type, suggesting a redundant inhibitory function of G6PD5 and G6PD6 in the regulation of VLCFA accumulation and related signaling. Contrasting with their roles in cancer cell proliferation, our study unveils novel insights into the G6PD signaling pathway, highlighting its unique function in negatively regulating plant callus formation.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109526"},"PeriodicalIF":6.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Argonaute 2 regulates nuclear DNA damage, repair, and phenotypes in Arabidopsis under genotoxic stress.","authors":"Jin-Hong Kim, Shubham Kumar Dubey, Tae Ho Ryu, Seung Sik Lee, Byung Yeoup Chung","doi":"10.1016/j.plaphy.2025.109528","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109528","url":null,"abstract":"<p><p>Argonaute (AGO) proteins are involved in gene expression and genome integrity during biotic and abiotic stress responses. AGO2 mediates double-strand break (DSB) repair in DNA damage response (DDR) induced by genotoxic stress. However, beyond DSB repair, the involvement of AGO proteins in DDR remains unknown. To investigate the potential roles and functions of AGO2 in DDR, we exposed three different ago2 mutants, each harboring a T-DNA insertion in the promoter, the N-terminal domain of exon 2, or the P-element-induced wimpy testis (PIWI) domain of exon 3, to genotoxic stress, and examined their DDR phenotypes. DDR phenotypes, such as root cell death and growth inhibition following γ-irradiation and zeocin treatment, were significantly suppressed by defects in the promoter or N-terminal domain of AGO2 but not by defects in the PIWI domain, which is responsible for RNA silencing. The weak DDR phenotypes were rescued by AGO2 overexpression and were attributed to reduced nuclear DNA damage despite impaired DNA repair, including DSB repair, as shown in comet and γH2AX assays. These results suggest that AGO2 regulates overall nuclear DNA damage and DDR phenotypes beyond DSB repair through the N-terminal domain rather than the PIWI domain. The potential role of AGO2 in the DDR implies that DNA repair may not be the primary factor for determining susceptibility to genotoxic stress.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109528"},"PeriodicalIF":6.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How do trees fail in intraspecific competition? A test for the roles of non-structural carbohydrates and stoichiometries in Pinus massoniana.","authors":"Guiwu Zou, Kang Xu, Junhuo Cai, Qingpei Yang, Jun Liu, Yuanqiu Liu, Xin Chen, Genxuan Wang","doi":"10.1016/j.plaphy.2025.109530","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109530","url":null,"abstract":"<p><p>Competition is ubiquitous and an important driver of tree mortality. Non-structural carbohydrates (NSCs, including soluble sugars and starch) and C-N-P stoichiometries are affected by the competitive status of trees and, in turn, physiologically determine tree growth and survival in competition. However, the physiological mechanisms behind tree mortality caused by intraspecific competition remain unclear. Here, we ask how the performance (growth vigour) of trees in intraspecific competition relates to NSC and C-N-P stoichiometry traits. Through the field surveys at neighbourhood levels, we demonstrated that competition is responsible for tree mortality in an even-aged Pinus massoniana forest. The whole NSCs and C-N-P stoichiometries of trees in different growth vigour classes (i.e., flourishing, moderate, and dying) were then analysed to elucidate how trees fail in competition. We found that (1) the concentrations of NSCs and their components in stems, coarse roots and fine roots were constant across tree growth vigour classes, but were significantly lower in the leaves, twigs and branches of moderate and dying trees than those of flourishing trees, and (2) the C, N and P concentration and their respective ratios were constant in all the tissues across tree growth vigour classes, but the nitrogen stoichiometric homeostasis index (H_N) of flourishing trees was significantly higher than that of moderate and dying trees. The results demonstrated that both carbohydrate deficiency and low stoichiometric homeostasis are potential physiological drivers underlying tree mortality caused by intraspecific competition. This study also emphasizes the importance of considering stoichiometric homeostasis in research on tree competition and forest dynamics.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109530"},"PeriodicalIF":6.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143040285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the bHLH gene family and functional analysis of ChMYC2 in drought stress of Cerasus humilis.","authors":"Buming Dong, Shaoyu Lang, Yongmei Gu, Xin Liu, Xingshun Song","doi":"10.1016/j.plaphy.2025.109519","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109519","url":null,"abstract":"<p><p>The basic helix-loop-helix (bHLH) transcription factors (TFs) play a crucial regulatory role in the growth and development of plants, as well as in their response to environmental stresses. In this study, we identified 94 ChbHLHs from Cerasus humilis, an economically valuable tree native to northern China. We analyzed their evolutionary relationships, gene structures, chromosome distributions, promoter cis-regulatory elements, and collinearity. Our analysis revealed numerous cis-regulatory elements associated with phytohormone responses and abiotic stress within the upstream promoter sequences of ChbHLH genes. The transcriptome results indicated that 84 ChbHLHs exhibited differential expression under drought conditions. Among those with upregulated expression levels, we selected ChMYC2 (ChbHLH93) for further investigation. Overexpressing ChMYC2 in Arabidopsis thaliana led to significantly elevated expression of drought-responsive genes compared to wild-type (WT) plants, resulting in enhanced drought resistance. Furthermore, we identified a gene, ChABI5 (ABA-insensitive 5), which interacts with ChMYC2. This study provides valuable genetic resources for future cultivation efforts aimed at developing stress-resistant and economically viable trees.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109519"},"PeriodicalIF":6.1,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the impact of NOS-derived NO on nitrogen metabolism and carbon flux in the heterocytous cyanobacterium Aphanizomenon flos-aquae 2012/KM1/D3.","authors":"Neha Gupta, Ankit Srivastava, Arun Kumar Mishra","doi":"10.1016/j.plaphy.2025.109515","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109515","url":null,"abstract":"<p><p>Nitric oxide synthases (NOSs) are heme-based monooxygenases that catalyze the NADPH-dependent oxidation of L-arginine to produce NO and L-citrulline. Over the past five years, the identification and characterization of NOS homologs in cyanobacteria have significantly advanced our understanding of these enzymes. However, the precise mechanisms through which NOS-derived NO influences nitrogen metabolism remain incompletely elucidated. Therefore, the present study aims to investigates the impact of NOS-derived NO on nitrogen metabolism, heterocyte development, and carbon utilization dynamics in Aphanizomenon flos-aquae. Results demonstrate a three-fold increase in NOS-dependent NO production during the log to stationary growth phase in reponse to L-arginine availability. This increase in NOS activity substantially impacted critical cellular processes related to nitrogen metabolism. Specifically, the inhibition of NOS activity disrupted regulatory mechanisms involving ntcA and glnB genes, resulting in a failure to induce hetR, hep, dev and nif genes necessary for heterocyte differentiation and nitrogenase synthesis. NOS-derived NO also played a pivotal role in modulating the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway, as evidenced by the sharp decline in glutamine and glutamate levels under NOS inhibition, which indicates impaired nitrogen assimilation. Besides, the observed alterations in succinate, fumarate, malate and pyruvate suggest regulatory roles of NOS in energy metabolism. NOS-inhibited cells redirected carbon flux towards glycogen/lipid biosynthesis, alongside protein degradation causing chlorosis, indicating nitrogen deficiency and compromised cellular viability. In contrast, NOS elicitation enhanced metabolic activity, supporting nitrogen assimilation and cellular growth. Overall, our results revealed the complex relationship among NOS-derived NO signaling, nitrogen metabolism, and carbon flux in cyanobacteria.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109515"},"PeriodicalIF":6.1,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of small RNA, transcriptome, and degradome sequencing: Mapping the miRNA-gene regulatory network for the development of sweet potato tuber roots.","authors":"Qiang Wu, Yuxi Chen, Wenqing Bi, Bin Tong, Aiqin Wang, Jie Zhan, Longfei He, Dong Xiao","doi":"10.1016/j.plaphy.2025.109510","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109510","url":null,"abstract":"<p><p>As an important starch crop, sweet potato has significant practical importance for maintaining food security worldwide. This study identified differential expressed genes associated with the expansion of tuberous roots by comparing the transcriptome across tuberous roots at the initial period (initiated tuberous roots (ITRs), rapid expansion period (tuberous roots (TRs), fibrous roots (FRs) at the seedling stage, and fibrous roots at the adult stage (unexpanded FRs (UFRs)). sRNA-seq and degradome analyses were performed to reveal the role of miRNAs in tuberous root development in sweet potato. A total of 29,633 genes and 510 miRNAs were differential expressed among FRs, ITRs, TRs, and UFRs. Integrated analyses of these data revealed genes involved in metabolism, hormone response, and signal transduction that might participate in the induction of tuberous root formation, while genes involved in carbohydrate and energy metabolism that might participate in the tuberous root swelling. A joint analysis of miRNAs and DEGs related to tuber development revealed by degradome-seq identified twelve miRNA-target gene pairs involved in gene expression process, hormone response, and metabolism of secondary metabolites that might be key regulators of root tuber development in sweet potato. Moreover, the functions of many miRNA-target gene pairs involved in the initiation of root tuber were related to auxin signaling response, and an exogenous hormone treatment experiment was further performed. The results indicated that auxin treatment had the most significant effect on increasing sweet potato yield, suggesting a dominant role of the auxin pathway in the regulation of sweet potato tuberous root development. Additionally, two miRNA-target pairs, miR319-TCP4 and miR172-AP2, which were identified from the degradome, were verified via 5' RNA ligase-mediated rapid amplification of cDNA ends (RLR-RACE) and tobacco transient cotransformation tests, and their expression was impacted by auxin treatment, which further validated the reliability of our multiomics analysis results. Our research provides new insights into the role of miRNAs in sweet potato root tuber development.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109510"},"PeriodicalIF":6.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated GWAS, BSA-seq, and RNA-seq analyses to identify candidate genes associated with male fertility trait in peach.","authors":"Zhenyu Huang, Hangling Bie, Ming Li, Lehan Xia, Long Chen, Yuling Chen, Lirong Wang, Zengyu Gan, Ke Cao","doi":"10.1016/j.plaphy.2025.109525","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109525","url":null,"abstract":"<p><p>Male sterility in peach (Prunus persica L.), characterized by the absence of fertile pollen grains in the anther, is determined by a recessive allele in homozygosis of the major gene located on chromosome 6. Developing tightly linked molecular markers can help identify appropriate peach parents or male-sterile plants for early culling in segregating progenies, thereby increasing breeding efficiency. In this study, we performed comprehensive research integrating genome-wide association study, bulked segregant analysis, and tissue-specific transcriptome sequencing for precisely characterizing the genes associated with male sterility and fertility in peach. We identified the candidate gene Prupe.6G027000, which encodes an ATP-binding cassette transporter G family member 26 (ABCG26), as a reliable candidate for controlling the targeted traits, as indicated by gene expression profiling and validated by quantitative real-time polymerase chain reaction, in situ hybridization, and virus-induced gene silencing. Prupe.6G027000 was transcribed preferentially on the tapetum and microspore surface, and its transient silencing caused severe pollen abortion in peach. The genotypes of nonsynonymous single-nucleotide variation (T > C) harbored in the coding region of Prupe.6G027000 exhibited approximately 96.2% consistency with male fertile or sterile phenotype in 579 peach accessions. These findings lay the foundation for dissecting the genetic basis of male fertility traits, and facilitating the establishment of a marker-assisted selection system in peaches.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109525"},"PeriodicalIF":6.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}