Plant Physiology and Biochemistry最新文献_第3页

Comparative network analysis reveals the regulatory mechanism of 1-methylcyclopropene on sugar and acid metabolisms in yellow peach stored at non-chilling temperatures 比较网络分析揭示了 1-甲基环丙烯对非冷藏温度下贮藏的黄桃中糖和酸代谢的调控机制

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-09-06 DOI: 10.1016/j.plaphy.2024.109100

{"title":"Comparative network analysis reveals the regulatory mechanism of 1-methylcyclopropene on sugar and acid metabolisms in yellow peach stored at non-chilling temperatures","authors":"","doi":"10.1016/j.plaphy.2024.109100","DOIUrl":"10.1016/j.plaphy.2024.109100","url":null,"abstract":"<div>Soluble carbohydrates and organic acids are critical determinants of fruit flavor and consumer preference, both of which are susceptible to postharvest treatments and storage conditions. While the individual effectiveness of 1-methylcyclopropene (1-MCP) and non-chilling temperature storage in delaying fruit ripening and influencing flavor development has been established, their combined effects on peach storage traits remain unexplored. This study investigated the impact of 1-MCP combined with non-chilling temperature storage on the quality and flavor attributes of yellow peach. Our results revealed that 1-MCP treatment reduced ethylene production during storage and delayed ripening and softening by down-regulating ethylene biosynthesis and signaling genes. Transcriptomic analysis indicated that 1-MCP maintained higher levels of soluble carbohydrates by up-regulating sucrose phosphate synthase (PpSPS1/2) and sorbitol dehydrogenase (PpSDH1) while down-regulating hexokinase (PpHXK1). Concurrently, 1-MCP preserved citric and malic acid levels by suppressing aconitate hydratase (PpACO1) and inducing malate dehydrogenase (PpMDH1), thereby delaying flavor degradation. Co-expression network analysis implicated ethylene response factors (PpERFs) as major regulators of sugar and acid metabolisms genes, with PpERF19 potentially functioning as a key transcriptional controller. Overall, this study verified the efficacy of combined 1-MCP and non-chilling storage for yellow peach preservation, identified key 1-MCP-modulated genes involved in sugar and acid metabolisms, and provided insights into regulating peach flavor development via postharvest approaches.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142157897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of SpGSH1 and SpPCS1 overexpression or co-overexpression on cadmium accumulation in yeast and Spirodela polyrhiza SpGSH1和SpPCS1过表达或共重表达对酵母和螺旋藻镉积累的影响

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-09-05 DOI: 10.1016/j.plaphy.2024.109097

{"title":"Effects of SpGSH1 and SpPCS1 overexpression or co-overexpression on cadmium accumulation in yeast and Spirodela polyrhiza","authors":"","doi":"10.1016/j.plaphy.2024.109097","DOIUrl":"10.1016/j.plaphy.2024.109097","url":null,"abstract":"<div>Cadmium (Cd) is one of the most toxic elements to all organisms. Glutathione (GSH)-dependent phytochelatin (PC) synthesis pathway is considered an extremely important mechanism in Cd detoxification in plants. However, few studies have focused on the roles of glutamate-cysteine ligase (GSH1) and phytochelatin synthase (PCS1) in Cd accumulation and detoxification in plants. In this study, SpGSH1 and SpPCS1 were identified and cloned from Spirodela polyrhiza and analyzed their functions in yeast and S. polyrhiza via single- or dual-gene (SpGP1) overexpression. The findings of this study showed that SpGSH1, SpPCS1, and SpGP1 could dramatically rescue the growth of the yeast mutant Δycf1. In S. polyrhiza, SpGSH1 was located in the cytoplasm and could promote Mn and Ca accumulation. SpPCS1 was located in the cytoplasm and nucleus, mainly expressed in meristem regions, and promoted Cd, Fe, Mn, and Ca accumulation. SpGSH1 and SpPCS1 co-overexpression increased the Cd, Mn, and Ca contents. Based on the growth data of S. polyrhiza, it was recommended that biomass as the preferable indicator for assessing plant tolerance to Cd stress compared to frond number in duckweeds. Collectively, this study for the first time systematically elaborated the function of SpGSH1 and SpPCS1 for Cd detoxification in S. polyrhiza.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142149881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of epigenetic factors in flavonoid accumulation during plant cold adaptation 表观遗传因子参与植物冷适应过程中黄酮类化合物的积累

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-09-05 DOI: 10.1016/j.plaphy.2024.109096

引用次数: 0

Assembly strategies for microbe-material hybrid systems in solar energy conversion 太阳能转换中微生物-材料混合系统的组装策略

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-09-04 DOI: 10.1016/j.plaphy.2024.109091

引用次数: 0

Comprehensive identification and expression analyses of sugar transporter genes reveal the role of GmSTP22 in salt stress resistance in soybean 糖转运体基因的全面鉴定和表达分析揭示了 GmSTP22 在大豆抗盐胁迫中的作用

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-09-04 DOI: 10.1016/j.plaphy.2024.109095

{"title":"Comprehensive identification and expression analyses of sugar transporter genes reveal the role of GmSTP22 in salt stress resistance in soybean","authors":"","doi":"10.1016/j.plaphy.2024.109095","DOIUrl":"10.1016/j.plaphy.2024.109095","url":null,"abstract":"<div>The transport, compartmentation and allocation of sugar are critical for plant growth and development, as well as for stress resistance, but sugar transporter genes have not been comprehensively characterized in soybean. Here, we performed a genome-wide identification and expression analyses of sugar transporter genes in soybean in order to reveal their putative functions. A total of 122 genes encoding sucrose transporters (SUTs) and monosaccharide transporters (MSTs) were identified in soybean. They were classified into 8 subfamilies according to their phylogenetic relationships and their conserved motifs. Comparative genomics analysis indicated that whole genome duplication/segmental duplication and tandem duplication contributed to the expansion of sugar transporter genes in soybean. Expression analysis by retrieving transcriptome datasets suggested that most of these sugar transporter genes were expressed in various tissues, and a number of genes exhibited tissue-specific expression patterns. Several genes including GmSTP21, GmSFP8, and GmPLT5/6/7/8/9 were predominantly expressed in nodules, and GmPLT8 was significantly induced by rhizobia inoculation in root hairs. Transcript profiling and qRT-PCR analyses suggested that half of these sugar transporter genes were significantly induced or repressed under stresses like salt, drought, and cold. In addition, GmSTP22 was found to be localized in the plasma membrane, and its overexpression promoted plant growth and salt tolerance in transgenic Arabidopsis under the supplement with glucose or sucrose. This study provides insights into the evolutionary expansion, expression pattern and functional divergence of sugar transporter gene family, and will enable further understanding of their biological functions in the regulation of growth, yield formation and stress resistance of soybean.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142163527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspectives on nanomaterial-empowered bioremediation of heavy metals by photosynthetic microorganisms 光合微生物利用纳米材料对重金属进行生物修复的前景。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-09-03 DOI: 10.1016/j.plaphy.2024.109090

{"title":"Perspectives on nanomaterial-empowered bioremediation of heavy metals by photosynthetic microorganisms","authors":"","doi":"10.1016/j.plaphy.2024.109090","DOIUrl":"10.1016/j.plaphy.2024.109090","url":null,"abstract":"<div>Environmental remediation of heavy metals (HMs) is a crucial aspect of sustainable development, safeguarding natural resources, biodiversity, and the delicate balance of ecosystems, all of which are critical for sustaining life on our planet. The bioremediation of HMs by unicellular phototrophs harnesses their intrinsic detoxification mechanisms, including biosorption, bioaccumulation, and biotransformation. These processes can be remarkably effective in mitigating HMs, particularly at lower contaminant concentrations, surpassing the efficacy of conventional physicochemical methods and offering greater sustainability and cost-effectiveness. Here, we explore the potential of various engineered nanomaterials to further enhance the capacity and efficiency of HM bioremediation based on photosynthetic microorganisms. The critical assessment of the interactions between nanomaterials and unicellular phototrophs emphasised the ability of tailored nanomaterials to sustain photosynthetic metabolism and the defence system of microorganisms, thereby enhancing their growth, biomass accumulation, and overall bioremediation capacity. Key factors that could shape future research efforts toward sustainable nanobioremediation of HM are discussed, and knowledge gaps in the field have been identified. This study sheds light on the potential of nanobioremediation by unicellular phototrophs as an efficient, scalable, and cost-effective solution for HM removal.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0981942824007587/pdfft?md5=f7f5ad687745af1b284cf3299653ce83&pid=1-s2.0-S0981942824007587-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142146170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlled atmosphere as cold chain support for extending postharvest life in cabbage 将可控气氛作为延长甘蓝收获后寿命的冷链支持。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-09-03 DOI: 10.1016/j.plaphy.2024.109094

{"title":"Controlled atmosphere as cold chain support for extending postharvest life in cabbage","authors":"","doi":"10.1016/j.plaphy.2024.109094","DOIUrl":"10.1016/j.plaphy.2024.109094","url":null,"abstract":"<div>Postharvest management of cabbage relies on high-intensity cooling to control postharvest physiology, minimising quality loss despite incurring significant energy and environmental costs. As an alternative, we hypothesised that controlled atmosphere (CA) could allow increased storage temperature by supporting physiological regulation, while maintaining quality and reducing energy demand. This study examined the effect CA (1.5 kPa CO2 and 6 kPa O2) at 5 or 10 °C on cabbage quality, with the aim of proposing a more sustainable and resilient supply chain. CA treatment was effective at reducing head respiration at higher temperature, with CA/10 °C treatment achieving lower respiration rates than Control/5 °C. Improved head colour retention and maintenance of stump quality were observed in cabbage under CA conditions. CA effects were seen also at a regulatory level; CA promoted an average of 25.4% reduction in abscisic acid accumulation potentially as part of a wider hypoxia stress response and was successful in decreasing expression of the senescence-coordinating transcription factor BoORE15. This finding was linked with a lower in downstream expression of pheophytinase and subtilisin protease. These results demonstrated that CA treatment fundamentally modified postharvest physiology in cabbage, which can be exploited to enable storage at warmer temperatures, contributing to supply chains with lower energy demand and its associated environmental benefits.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0981942824007629/pdfft?md5=6bff38ac0ab0c52bafe00465797cb507&pid=1-s2.0-S0981942824007629-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142146167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sodium silicate accelerates suberin accumulation at wounds of potato tuber by inducing phenylpropanoid pathway and fatty acid metabolism during healing 硅酸钠通过诱导愈合过程中的苯丙醇途径和脂肪酸代谢，加速马铃薯块茎伤口处的单宁积累。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-09-03 DOI: 10.1016/j.plaphy.2024.109093

{"title":"Sodium silicate accelerates suberin accumulation at wounds of potato tuber by inducing phenylpropanoid pathway and fatty acid metabolism during healing","authors":"","doi":"10.1016/j.plaphy.2024.109093","DOIUrl":"10.1016/j.plaphy.2024.109093","url":null,"abstract":"<div>Although soluble silicate was reported to accelerate wound healing in muskmelon fruit through encouraging the deposition of lignin or free fatty acids, whether sodium silicate affects the biosynthesis, cross-linking and transport of suberin monomers during potato wound healing remains unknown. In this study, sodium silicate upregulated the expression and activity of 4-coumarate: coenzyme A ligase (4CL), phenylalanine ammonia lyase (PAL), and promoted the synthesis of phenolic acids (caffeic acid, p-coumaric acid, cinnamic acid, sinapic acid, and ferulic acid) in tuber wounds. Meanwhile, sodium silicate upregulated the expression of glycerol-3-phosphate acyltransferase (StGPAT), fatty acyl reductase (StFAR), long-chain acyl-CoA synthetase (StLACS), β-ketoacyl-CoA synthase (StKCS), and cytochrome P450 (StCYP86A33), and thus increased the levels of α, ω-diacids, ω-hydroxy acids, and primary alcohols in wounds. Sodium silicate also induced the expression of ω-hydroxy acid/fatty alcohol hydroxycinnamoyl transferase (StFHT), ABC transporter (StABCG), and promoted the deposition of suberin in wound surface, hence reducing tuber disease index and weight loss during healing. Taken together, sodium silicate may accelerate suberin accumulation at potato tubers wound through inducing the phenylpropanoid pathway and fatty acid metabolism.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142146172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal mechanisms and characteristics of Pseudomonas fluorescens: Promoting peanut growth and combating Fusarium oxysporum-induced root rot 荧光假单胞菌的抗真菌机制和特性：促进花生生长和防治镰孢菌诱发的根腐病。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-09-03 DOI: 10.1016/j.plaphy.2024.109092

{"title":"Antifungal mechanisms and characteristics of Pseudomonas fluorescens: Promoting peanut growth and combating Fusarium oxysporum-induced root rot","authors":"","doi":"10.1016/j.plaphy.2024.109092","DOIUrl":"10.1016/j.plaphy.2024.109092","url":null,"abstract":"<div>Continuous cropping of peanuts presents significant challenges to sustainable production due to soil-borne diseases like root rot caused by Fusarium species. In this study, field inoculation experiments treatments and in vitro agar plate confrontation tests were conducted, including non-inoculated controls (CK), inoculation with Pseudomonas fluorescens (PF), Fusarium oxysporum (FO), and co-inoculation with both (PF + FO). The aim was to explore the antifungal mechanisms of Pseudomonas fluorescens in mitigating root rot and enhancing peanut yield. The results indicated that PF and PF + FO significantly enhanced peanut root activity, as well as superoxide dismutase, catalase, and glutathione S-transferase activities, while simultaneously decreasing the accumulation of reactive oxygen species and malondialdehyde contents, compared to FO treatment. Additionally, PF treatment notably increased lignin content through enhanced phenylalanine ammonia lyase, cinnamate 3-hydroxylase, and peroxidase activity compared to CK and FO treatment. Moreover, PF treatment resulted in longer roots and a higher average diameter and surface area, potentially due to increased endogenous levels of auxin and zeatin riboside, coupled with decreased abscisic acid content. PF treatment significantly elevated chlorophyll content and the maximum photochemical efficiency of PSII in the light-adapted state, the actual photochemical efficiency and the proportion of PSII reaction centers open, leading to improved photosynthetic performance. Confrontation culture assays revealed PF's notable inhibitory effects on Fusarium oxysporum growth, subsequently reducing rot disease incidence in the field. Ultimately, PF treatment led to increased peanut yield by enhancing plant numbers and pod weight compared to FO treatment, indicating its potential in mitigating Fusarium oxysporum-induced root rot disease under continuous cropping systems.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142146166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of induction, system optimization and genetic transformation in Veratrum californicum var. vitro cultures to enhance the production of cyclopamine and veratramine 在加州马鞭草变种体外培养中整合诱导、系统优化和遗传转化，以提高环丙胺和维拉曲明的产量。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-09-02 DOI: 10.1016/j.plaphy.2024.109087

{"title":"Integration of induction, system optimization and genetic transformation in Veratrum californicum var. vitro cultures to enhance the production of cyclopamine and veratramine","authors":"","doi":"10.1016/j.plaphy.2024.109087","DOIUrl":"10.1016/j.plaphy.2024.109087","url":null,"abstract":"<div>Cyclopamine, a compound found in wild Veratrum has shown promising potential as a lead anti-cancer drug by effectively blocking cancer signaling pathways. However, its complex chemical structure poses challenges for artificial synthesis, thus limiting its supply and downstream drug production. This study comprehensively utilizes induction, system optimization, and transgenic technologies to establish an efficient suspension culture system for the high-yield production of cyclopamine and its precursor, veratramine. Experimental results demonstrate that methyl jasmonate (MeJA) effectively promotes the content of veratramine and cyclopamine in Veratrum californicum var. callus tissue, while yeast extract (YE) addition significantly increases cell biomass. The total content of veratramine and cyclopamine reached 0.0638 mg after synergistic treatment of suspension system with these two elicitors. And the content of the two substances was further increased to 0.0827 mg after the optimization by response surface methodology. Subsequently, a genetic transformation system for V. californicum callus was established and a crucial enzyme gene VnOSC1, involved in the steroidal alkaloid biosynthesis pathway, was screened and identified for genetic transformation. Combined suspension culture and synergistic induction system, the total content of the two substances in transgenic suspension system was further increased to 0.1228 mg, representing a 276.69% improvement compared to the initial culture system. This study proposes a complete and effective genetic transformation and cultivation scheme for V. californicum tissue cells, achieving milligram-level production of the anticancer agent cyclopamine and its direct precursor veratramine for the first time. It provides a theoretical basis for the industrial-scale production of these substances.</div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142146168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0