Plant Physiology and Biochemistry最新文献_第4页

Integrated GWAS, BSA-seq, and RNA-seq analyses to identify candidate genes associated with male fertility trait in peach. 综合GWAS、BSA-seq和RNA-seq分析鉴定桃树雄性育性性状相关候选基因。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-01-17 DOI: 10.1016/j.plaphy.2025.109525

Zhenyu Huang, Hangling Bie, Ming Li, Lehan Xia, Long Chen, Yuling Chen, Lirong Wang, Zengyu Gan, Ke Cao

{"title":"Integrated GWAS, BSA-seq, and RNA-seq analyses to identify candidate genes associated with male fertility trait in peach.","authors":"Zhenyu Huang, Hangling Bie, Ming Li, Lehan Xia, Long Chen, Yuling Chen, Lirong Wang, Zengyu Gan, Ke Cao","doi":"10.1016/j.plaphy.2025.109525","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109525","url":null,"abstract":"Male sterility in peach (Prunus persica L.), characterized by the absence of fertile pollen grains in the anther, is determined by a recessive allele in homozygosis of the major gene located on chromosome 6. Developing tightly linked molecular markers can help identify appropriate peach parents or male-sterile plants for early culling in segregating progenies, thereby increasing breeding efficiency. In this study, we performed comprehensive research integrating genome-wide association study, bulked segregant analysis, and tissue-specific transcriptome sequencing for precisely characterizing the genes associated with male sterility and fertility in peach. We identified the candidate gene Prupe.6G027000, which encodes an ATP-binding cassette transporter G family member 26 (ABCG26), as a reliable candidate for controlling the targeted traits, as indicated by gene expression profiling and validated by quantitative real-time polymerase chain reaction, in situ hybridization, and virus-induced gene silencing. Prupe.6G027000 was transcribed preferentially on the tapetum and microspore surface, and its transient silencing caused severe pollen abortion in peach. The genotypes of nonsynonymous single-nucleotide variation (T > C) harbored in the coding region of Prupe.6G027000 exhibited approximately 96.2% consistency with male fertile or sterile phenotype in 579 peach accessions. These findings lay the foundation for dissecting the genetic basis of male fertility traits, and facilitating the establishment of a marker-assisted selection system in peaches.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109525"},"PeriodicalIF":6.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic molecular dissection of key intermediates in xanthones biosynthesis in Cudrania tricuspidata and establishment of a viable heterologous expression system. 三尖杉黄酮生物合成关键中间体的系统分子剖析和可行异源表达系统的建立。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-01-17 DOI: 10.1016/j.plaphy.2025.109524

Jing Zhang, Dan-Dan Xu, Ting-Ting Zhu, He Ta, Yue Hao, Jiao-Zhen Zhang, Jie Fu, Ai-Xia Cheng, Hong-Xiang Lou

{"title":"Systematic molecular dissection of key intermediates in xanthones biosynthesis in Cudrania tricuspidata and establishment of a viable heterologous expression system.","authors":"Jing Zhang, Dan-Dan Xu, Ting-Ting Zhu, He Ta, Yue Hao, Jiao-Zhen Zhang, Jie Fu, Ai-Xia Cheng, Hong-Xiang Lou","doi":"10.1016/j.plaphy.2025.109524","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109524","url":null,"abstract":"The major phytochemicals in the roots of Cudrania tricuspidata are prenylated xanthones, exhibiting significant structural diversity and bioactive properties, such as anti-inflammatory, antioxidative, and antitumor effects. The biosynthetic pathways of these compounds have not yet been resolved, limiting their production through synthetic biology. In this study, benzoyl-coenzyme A (CoA) ligase (BZL), benzophenone synthase (BPS), and benzophenone 3'-hydroxylase (B3'H) transcripts involved in the biosynthesis of xanthone were cloned and characterized from C. tricuspidata. The results showed that C. tricuspidata BZL (CtBZL) catalyzed the formation of benzoyl-CoA from benzoate and C. tricuspidata BPS (CtBPS) catalyzed the condensation of benzoyl-CoA and three molecules of malonyl-CoA to form 2,4,6-trihydroxybenzophenone (2,4,6-triHB) via Claisen type cyclization. Then, C. tricuspidata B3'H (CtB3'H) hydroxylated 2,4,6-triHB to produce 2,3',4,6-tetrahydroxybenzophenone (2,3',4,6-tetraHB), which was a key precursor of xanthone derivatives. In addition, the functions of the enzymes were further explored by the heterologous expression of CtBZL, CtBPS, and CtB3'H in Saccharomyces cerevisiae and Nicotiana benthamiana. Also, a preliminary heterologous synthesis system of 2,3',4,6-tetraHB was established. The present study provided the candidate genes and strategies for the heterologous production of xanthones using S. cerevisiae and N. benthamiana as hosts.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109524"},"PeriodicalIF":6.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of anaplerotic enzymes by melatonin enhances resilience to cadmium toxicity in Vigna radiata (L.) R. Wilczek.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-01-17 DOI: 10.1016/j.plaphy.2025.109522

M Nasir Khan, Shaistul Islam, Manzer H Siddiqui

{"title":"Regulation of anaplerotic enzymes by melatonin enhances resilience to cadmium toxicity in Vigna radiata (L.) R. Wilczek.","authors":"M Nasir Khan, Shaistul Islam, Manzer H Siddiqui","doi":"10.1016/j.plaphy.2025.109522","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109522","url":null,"abstract":"Melatonin (Mel) is a tryptophan-derived (N-acetyl-5-methoxytryptamine) molecule. In the present study, role of Mel in the regulation of various anaplerotic enzymes is discussed in relation to N metabolism and H+-ATPase activity in mung bean under Cd stress. The application of Mel to the Cd-stressed mung bean seedlings was remarkable in improving the activity of hexokinase (35.7%), pyruvate kinase (79.2%), phosphoenolpyruvate carboxylase (38.9%) pyruvate dehydrogenase (41.5%), malate dehydrogenase (49.2%), citrate synthase (37.7%), isocitrate dehydrogenase (33.1%), ATP synthase (63.6%), and ATPase (38.6%). Incubation of Cd-stressed seedlings with Mel also improved the activity of nitrate reductase by 89.4%, nitrite reductase by 78.2%, and glutamine synthetase by 35.3% that resulted in higher level of ammonium and their subsequent assimilation to amino acids and proteins. Activation of these enzymes was strongly associated with Mel-induced regulation of H+-ATPase activity that improved K+ retention and N assimilation capacity of the Cd-stressed seedlings of mung bean. The coordinated mechanism of action of tricarboxylic acid (TCA) cycle, N metabolism, and higher K+ levels were helpful in providing protection against detrimental effects of Cd toxicity through improving the defense system and energy level of the plants. However, inclusion of sodium orthovanadate (PM H+-ATPase inhibitor) to the incubation medium reversed the positive effect of Mel and suppressed the performance of plants under Cd-stress. The findings of the study indicate that under Cd stress, the regulatory mechanisms of anaplerotic enzymes and antioxidant defense are mediated by Mel, and this process is facilitated by the retention of K+ induced by H+-ATPase.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109522"},"PeriodicalIF":6.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene expression and mucilage adaptations to salinity in germination of extreme halophyte Schrenkiella parvula seeds. 极端盐生植物小叶雪莲种子萌发过程中基因表达及粘液对盐度的适应。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-01-17 DOI: 10.1016/j.plaphy.2025.109517

Keriman Şekerci, Nahoko Higashitani, Rengin Ozgur, Atsushi Higashitani, Ismail Turkan, Baris Uzilday

{"title":"Gene expression and mucilage adaptations to salinity in germination of extreme halophyte Schrenkiella parvula seeds.","authors":"Keriman Şekerci, Nahoko Higashitani, Rengin Ozgur, Atsushi Higashitani, Ismail Turkan, Baris Uzilday","doi":"10.1016/j.plaphy.2025.109517","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109517","url":null,"abstract":"Salinization is a significant global issue causes irreversible damage to plants by reducing osmotic potential, inhibiting seed germination, and impeding water uptake. Seed germination, a crucial step towards the seedling stage is regulated by several hormones and genes, with the balance between abscisic acid and gibberellin being the key mechanism that either promotes or inhibits this process. Additionally, mucilage, a gelatinous substance, is known to provide protection against drought, herbivory, soil adhesion, and seed sinking. However, limited information is available on the structure and thickness of seed mucilage in halophytes under different salinity conditions. In this study, the mucilage structure of the extreme halophyte Schrenkiella parvula was compared with the glycophyte Arabidopsis thaliana in response to salinity. We found differences in the expression levels of genes such as ABI5, RGL2, DOG1, ENO2, and DHAR2, which are involved in seed germination and antioxidant activity, as well as in the mucilage structure of seeds of S. parvula and A. thaliana seeds at different salt concentrations. The responses of seed germination of S. parvula to salinity indicate that it is more salt-tolerant than A. thaliana. Additionally, it was found that S. parvula mucilage decreased under salt conditions but not under mannitol conditions, whereas in A. thaliana mucilage did not change under both conditions, which is one of the adaptation strategies of S. parvula to salt conditions. We believe that these fundamental analyzes will provide a foundation for future molecular and biochemical studies comparing the responses of crops and halophytes to salinity stress.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109517"},"PeriodicalIF":6.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyethylene nanoplastics affected morphological, physiological, and molecular indices in tomato (Solanum lycopersicum L.). 聚乙烯纳米塑料对番茄形态、生理和分子指标的影响。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-01-17 DOI: 10.1016/j.plaphy.2025.109523

Masoumeh Nazari, Alireza Iranbakhsh, Mostafa Ebadi, Zahra Oraghi Ardebili

{"title":"Polyethylene nanoplastics affected morphological, physiological, and molecular indices in tomato (Solanum lycopersicum L.).","authors":"Masoumeh Nazari, Alireza Iranbakhsh, Mostafa Ebadi, Zahra Oraghi Ardebili","doi":"10.1016/j.plaphy.2025.109523","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109523","url":null,"abstract":"This study explored morphological, physiological, molecular, and epigenetic responses of tomatoes (Solanum lycopersicum) to soil contamination with polyethylene nanoplastics (PENP; 0.01, 0.1, and 1 gkg-1 soil). The PENP pollution led to severe changes in plant morphogenesis. The PENP treatments were associated with decreased plant biomass, reduced internode length, delayed flowering, and prolonged fruit ripening. Abnormal inflorescences, flowers, and fruits observed in the PENP-exposed seedlings support genetic changes and meristem dysfunction. Exposure of seedlings to PENP increased H2O2 accumulation and damaged membranes, implying oxidative stress. The PENP treatments induced activities of catalase (EC1.11.1.6), peroxidase (EC1.11.1.7), and phenylalanine ammonia-lyase (EC4.3.1.24) enzymes. Soil contamination with PENP also decreased the net photosynthesis, maximum photosystem efficiency, stomatal conductance, and transpiration rate. The nano-pollutant upregulated the expression of the histone deacetylase (HDA3) gene and R2R3MYB transcription factor. However, the AP2a gene was down-regulated in response to the PENP treatment. Besides, EPNP epigenetically contributed to changes in DNA methylation. The concentrations of proline, soluble phenols, and flavonoids also displayed an upward trend in response to the applied PENP treatments. The long-term exposure of seedlings to PENP influenced fruit biomass, firmness, ascorbate, lycopene, and flavonoid content. These findings raise concerns about the hazardous aspects of PENP to agricultural ecosystems and food security.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109523"},"PeriodicalIF":6.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of molecularly diversified phosphorus-solubilizing rhizobacterial isolates in phytostimulation, antimicrobial attributes and phosphorus-transporter genes mediated plant growth performance in maize (Zea mays L.).

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-01-16 DOI: 10.1016/j.plaphy.2025.109521

Suman Sanadhya, Devendra Jain, Heena Saheewala, Deepak Sharma, Prabhat K Chauhan, Garima Singh, Sudhir K Upadhyay, Santosh Ranjan Mohanty

{"title":"Efficacy of molecularly diversified phosphorus-solubilizing rhizobacterial isolates in phytostimulation, antimicrobial attributes and phosphorus-transporter genes mediated plant growth performance in maize (Zea mays L.).","authors":"Suman Sanadhya, Devendra Jain, Heena Saheewala, Deepak Sharma, Prabhat K Chauhan, Garima Singh, Sudhir K Upadhyay, Santosh Ranjan Mohanty","doi":"10.1016/j.plaphy.2025.109521","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109521","url":null,"abstract":"This study evaluated a dual management approach to enhance plant-growth by improving soil fertility, reducing pathogenic stress using PGPR that affect phosphorus-transporter (pht) genes. Among 213 maize rhizobacterial isolates, 40 demonstrated the ability to solubilize tri-calcium phosphate, potassium, zinc, and silicon, showing various PGP traits. Nine of these isolates exhibited significant antagonistic activity against the plant pathogens Colletotrichum chlorophyti and Xanthomonas axonopodis. These pathogens cause root infection, reduces plant-immunity and growth. In pot experiments, these nine strains significantly improved root length, shoot length, chlorophyll content, fresh weight, proline, APX, CAT, GR, NPK, and Zn content in maize plants after 60 days under pathogenic stress. Notably, PSB-25 increased root length by up to 66% under C. chlorophyti stress and 64% under X. axonopodis stress. PSB21 enhanced proline content by 49%, APX by 70%, and GR by 41%, while PSB-16 raised CAT activity by 55% under X. axonopodis stress. Molecular diversity analysis of the 40 PS-RB strains using ERIC, BOX, REP, and ARDRA showed two major clusters with Jaccard coefficients from 0.72 to 1.00. 16S rRNA gene sequencing identified PSB10, PSB16, and PSB25 as Serratia sp., Enterobacter cloacae, and Enterobacter sp., respectively. The effects of PSB10, PSB16, and PSB25 on growth parameters under pathogen stress were also studied. Field trials indicated that treatment T6 (100% RDF + PSB16) was most effective in promoting plant growth. Additionally, significant differences in the expression of six Pht1 transporter genes were noted between PS-RB treated and untreated maize seedlings, and these genes improving phosphorus acquisition.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109521"},"PeriodicalIF":6.1,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salicylic acid mitigates the physiological and biochemistry toxicity of fungicide difenoconazole and reduces its accumulation in wheat (Triticum aestivum L.). 水杨酸能减轻杀菌剂异苯唑的生理生化毒性，减少其在小麦体内的积累。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-01-16 DOI: 10.1016/j.plaphy.2025.109504

Jingchong Li, Wende Zheng, Jingkun Li, Komelle Askari, Zhixiang Tian, Runqiang Liu

{"title":"Salicylic acid mitigates the physiological and biochemistry toxicity of fungicide difenoconazole and reduces its accumulation in wheat (Triticum aestivum L.).","authors":"Jingchong Li, Wende Zheng, Jingkun Li, Komelle Askari, Zhixiang Tian, Runqiang Liu","doi":"10.1016/j.plaphy.2025.109504","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109504","url":null,"abstract":"Continuous misuse of difenoconazole (DFZ) results in farmland contamination, posing risks to crops and human health. Salicylic acid (SA) has been shown to enhance plant resistance and reduce pesticide phytotoxicity and accumulation. However, whether SA effectively reduces DFZ phytotoxicity and accumulation and its underlying mechanisms remain poorly understood. To address this, a short-term indoor experiment and a long-term outdoor pot experiment were conducted to evaluate the potential of SA to alleviate DFZ-induced phytotoxicity and its effects on DFZ uptake, translocation, metabolism, and accumulation. The underlying mechanisms were explored through physiological, biochemical, and gene expression analyses. The results showed that DFZ induced oxidative damage and reduced photosynthesis by 15.6% in wheat. SA upregulated the expression of genes encoding antioxidant enzymes (POD, CAT, SOD1, and SOD2) in the roots and leaves of DFZ-exposed plants, leading to a 7.5%-13.4% increase in antioxidant enzyme activities and a subsequent 9.7%-14.5% decrease in reactive oxygen species levels. Additionally, SA increased the total chlorophyll content by 16.3%, which was enhanced by regulating chlorophyll synthesis and degradation-related genes, thereby improving the net photosynthetic rate by 12.2%. Furthermore, SA upregulated the expression of lignin biosynthesis-related, CYP450, and GST genes, which reduced DFZ uptake and accelerated its degradation. Consequently, the wheat grain DFZ content decreased by 36.2%, thus reducing the health risk index. This study confirms the potential of SA to reduce DFZ phytotoxicity and accumulation. Based on these findings, we recommend using SA in DFZ-contaminated areas to mitigate phytotoxicity and the associated human dietary exposure risks.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109504"},"PeriodicalIF":6.1,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of cadmium pathways in a high Cd accumulating cultivar versus a low Cd accumulating cultivar of Theobroma cacao L.

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-01-16 DOI: 10.1016/j.plaphy.2025.109511

Hester Blommaert, Anne-Marie Aucour, Matthias Wiggenhauser, Sylvain Campillo, Claudia Moens, Hiram Castillo-Michel, Jesse Dekeyrel, Pathmanathan Umaharan, Erik Smolders, Géraldine Sarret

{"title":"Comparison of cadmium pathways in a high Cd accumulating cultivar versus a low Cd accumulating cultivar of Theobroma cacao L.","authors":"Hester Blommaert, Anne-Marie Aucour, Matthias Wiggenhauser, Sylvain Campillo, Claudia Moens, Hiram Castillo-Michel, Jesse Dekeyrel, Pathmanathan Umaharan, Erik Smolders, Géraldine Sarret","doi":"10.1016/j.plaphy.2025.109511","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109511","url":null,"abstract":"Understanding cadmium (Cd) pathways in cacao trees is critical for developing Cd mitigation strategies. This study investigates whether Cd uptake and translocation mechanisms differ between a low and a high Cd-accumulating cacao cultivar. We sampled three replicate trees of each cultivar, and a grafted cultivar that shared the same scion as the low Cd accumulator but had a different rootstock. All cultivars grew in the same field with similar bioavailable soil Cd. We utilized Cd stable isotope analyses to trace Cd pathways within the trees, complemented by micro-scale imaging of Cd distribution in leaves and branches, and nutrient and Cd quantification across tree organs. The high Cd accumulator exhibited 2.9-fold higher Cd uptake than the low Cd accumulator, while the grafted cultivar showed 1.7-fold higher uptake. These differences matched Mn uptake. The δ114/110Cd values of organs increased in the order: roots ≤ nibs, young leaves ≤ branches ≤ pod husks < mature leaves for the high and grafted cultivar, and nibs ≤ roots ≤ branches ≤ pod husks < mature leaves for the low cultivar. The enrichment in heavy isotopes correlated with a progressive retention of Cd compared to Mn along the xylem pathway from roots to branches to leaves. The differences in Cd isotope compositions between cultivars indicate that there are differences in translocation processes, yet they did not affect the relative Cd internal distribution. Cd mass balances and internal translocation factors (ITF) corroborated that differences in nib Cd concentrations among cultivars were primarily due to uptake rather than translocation.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109511"},"PeriodicalIF":6.1,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soybean tolerance to waterlogging is achieved by detoxifying root lactate via lactate dehydrogenase in leaves and metabolizing malate and succinate. 大豆耐涝是通过叶片乳酸脱氢酶脱毒根系乳酸并代谢苹果酸和琥珀酸实现的。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-01-16 DOI: 10.1016/j.plaphy.2025.109520

Douglas Antônio Posso, Eduardo Pereira Shimoia, Cristiane Jovelina da-Silva, An Nguyen Thuy Phan, Gabriela Niemeyer Reissig, Tamires da Silva Martins, Brigitta Ehrt, Patricia Dalcin Martins, Ana Claudia Barneche de Oliveira, Lars Mathias Blank, Junior Borella, Joost Thomas van Dongen, Luciano do Amarante

{"title":"Soybean tolerance to waterlogging is achieved by detoxifying root lactate via lactate dehydrogenase in leaves and metabolizing malate and succinate.","authors":"Douglas Antônio Posso, Eduardo Pereira Shimoia, Cristiane Jovelina da-Silva, An Nguyen Thuy Phan, Gabriela Niemeyer Reissig, Tamires da Silva Martins, Brigitta Ehrt, Patricia Dalcin Martins, Ana Claudia Barneche de Oliveira, Lars Mathias Blank, Junior Borella, Joost Thomas van Dongen, Luciano do Amarante","doi":"10.1016/j.plaphy.2025.109520","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109520","url":null,"abstract":"Waterlogging is a significant stressor for crops, particularly in lowland regions where soil conditions exacerbate the problem. Waterlogged roots experience hypoxia, disrupting oxidative phosphorylation and triggering metabolic reorganization to sustain energy production. Here, we investigated the metabolic aspects that differentiate two soybean sister lines contrasting for waterlogging tolerance. After 11 days of waterlogging, roots of the tolerant line (PELBR15-7015C) modulated their fermentative metabolism by exporting key metabolites (lactate, malate, and succinate) to the shoot. These metabolites were metabolized in the leaves, supporting photosynthesis and facilitating sugar export to the roots, sustaining a root-shoot-root cycling process. In contrast, the sensitive line (PELBR15-7060) entered a quiescent state, depleting its carbon stock and accumulating protective metabolites. Our study reveals that long-term waterlogging tolerance is primarily achieved through lactate detoxification in the leaves, along with malate and succinate metabolism, enabling root metabolism to withstand hypoxia. This mechanism offers new insights into crop resilience under waterlogged conditions, with implications for modern agriculture as climate change intensifies the frequency and duration of such stress events.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109520"},"PeriodicalIF":6.1,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-temperature-induced singlet oxygen adaptation decreases susceptibility to the mycotoxin TeA in invasive plant Ageratina adenophora. 低温诱导的单线态氧适应降低了入侵植物Ageratina adenophora对霉菌毒素TeA的敏感性。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2025-01-14 DOI: 10.1016/j.plaphy.2025.109508

Liru Mi, Haiou Liu, Jing Zhang, Yanjing Guo, Jiale Shi, Yuping Lu, Jing Cheng, He Wang, Dan Cheng, Bernal E Valverde, Sheng Qiang, Shiguo Chen

{"title":"Low-temperature-induced singlet oxygen adaptation decreases susceptibility to the mycotoxin TeA in invasive plant Ageratina adenophora.","authors":"Liru Mi, Haiou Liu, Jing Zhang, Yanjing Guo, Jiale Shi, Yuping Lu, Jing Cheng, He Wang, Dan Cheng, Bernal E Valverde, Sheng Qiang, Shiguo Chen","doi":"10.1016/j.plaphy.2025.109508","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109508","url":null,"abstract":"The mycotoxin tenuazonic acid (TeA) inhibits photosynthesis and is expected to be developed as a bioherbicide to control Ageratina adenophora that is one of the most serious invasive alien plants in China. New leaves sprouting from A. adenophora at low temperatures (LT) in early spring are less sensitive to TeA compared to those growing in summer. However, the molecular mechanism of LT-caused decrease in the susceptibility of A. adenophora to TeA is unclear. In this study, three singlet oxygen-responsive genes (SORGs) and three jasmonic acid responsive genes (JARGs) were cloned to further probe the role of singlet oxygen (1O2) signaling during TeA-induced disease development in A. adenophora leaves exposed to LT. TeA triggered chloroplast-derived 1O2 production as a result of photosystem II (PSII) photoinhibition during leaf lesion formation in A. adenophora. Moreover, TeA indeed induced the expression of SORGs and JARGs as well as a high level of JA generation, activating the 1O2 signaling pathway in A. adenophora. LT (12°C) pretreatment can cause PSII photoinhibition and increase the SORG AaAAA-ATPase expression level in A. adenophora leaves, meaning that 1O2 signaling was activated by LT. Thus TeA led to less increase of the SORGs and JARGs expression and JA level in plants pretreated by LT compared with non-pretreated plants, although both of them had the same level of 1O2 production after TeA treatment. It was concluded that the low susceptibility to TeA of A. adenophora subjected to LT can be attributed to the occurrence of 1O2 acclimation.","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109508"},"PeriodicalIF":6.1,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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