Plant Physiology and Biochemistry最新文献

{"title":"Combining plant growth-promoting bacteria as a tool to improve the metabolism and productivity of sugarcane","authors":"Mariley Fonseca ,&nbsp;João William Bossolani ,&nbsp;Israel Alves Filho ,&nbsp;Sirlene Lopes de Oliveira ,&nbsp;Tatiani Mayara Galeriani ,&nbsp;Fernando Dini Andreote ,&nbsp;Carlos Alexandre Costa Crusciol","doi":"10.1016/j.plaphy.2025.109980","DOIUrl":"10.1016/j.plaphy.2025.109980","url":null,"abstract":"<div><div>Sugarcane (<em>Saccharum</em> spp.) is a globally important crop, and strategies to minimize the negative impacts of its cultivation and enhance its development are highly relevant. Plant growth-promoting bacteria (PGPB) can sustainably foster plant growth in agricultural systems and mitigate adverse effects of stress on plants. This is the first study to investigate the combined use of <em>Azospirillum brasilense</em> (<em>Ab</em>) and <em>Nitrospirillum amazonense</em> (<em>Na</em>), two microorganisms widely applied in agricultural systems, aiming to elucidate their effects on the nutritional status, biochemical responses, and productive parameters of sugarcane. Greenhouse experiments were conducted under controlled water and temperature conditions with four treatments: application of <em>Ab, Na</em>, or <em>Ab</em> + <em>Na</em> (Mix) or no PGPB application (control). Sugarcane was cultivated until the middle of the rapid growth stage. To validate the results, the greenhouse trials were replicated under field conditions at two sites (Maracaí-SP and Pradópolis-SP). The results showed that the inoculation of sugarcane with plant growth-promoting bacteria (PGPB), particularly <em>Ab</em> and Mix, enhanced nutritional aspects, especially N content. These increases were significant under greenhouse (<em>p</em> ≤ 0.05) and field conditions (<em>p</em> ≤ 0.10). Additionally, inoculation reduced oxidative stress and improved photosynthetic parameters, such as net photosynthetic rate, water use efficiency, and carboxylation efficiency. These cascading effects contributed to significant gains in crop productivity, with an average increase in stalk yield of 10.9 % for <em>Ab</em> and 12.2 % for Mix across both environments. Similarly, there was an increase in sugar yield per hectare, with gains of 13.3 % for <em>Ab</em> and 13.7 % for Mix compared to the control. These findings highlight the potential of PGPB as a sustainable strategy to enhance crop productivity and resilience, contributing to environmentally balanced agricultural systems. Although the benefits of PGPB were evident, differences between <em>Ab</em> and Mix were not pronounced. Therefore, additional studies are needed to explore the potential of these combinations under adverse conditions, when their effects could be more pronounced.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"225 ","pages":"Article 109980"},"PeriodicalIF":6.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Lily (Lilium spp.) LhERF061 suppresses anthocyanin biosynthesis by inhibiting LhMYBSPLATTER and LhDFR expression and interacting with LhMYBSPLATTER” [2024 Nov 22:219:109325]","authors":"Yue Yang ,&nbsp;Mengmeng Bi ,&nbsp;Kang Luo ,&nbsp;Yuwei Cao ,&nbsp;Jing Wang ,&nbsp;Panpan Yang ,&nbsp;Leifeng Xu ,&nbsp;Jun Ming","doi":"10.1016/j.plaphy.2025.109496","DOIUrl":"10.1016/j.plaphy.2025.109496","url":null,"abstract":"","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109496"},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Genome-wide identification and characterization of alfalfa-specific genes in drought stress tolerance\" [Plant Physiol. Biochem. 220 (2025) 109474].","authors":"Yitong Ma, Qingyan Zhai, Zhipeng Liu, Wenxian Liu","doi":"10.1016/j.plaphy.2025.109776","DOIUrl":"10.1016/j.plaphy.2025.109776","url":null,"abstract":"","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":" ","pages":"109776"},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Optimization of apple fruit flavor by MdVHP1-2 via modulation of soluble sugar and organic acid accumulation\" [(206), January 2024, 108227].","authors":"Ying Xiang, Xiao-Yu Huang, Yu-Wen Zhao, Chu-Kun Wang, Quan Sun, Da-Gang Hu","doi":"10.1016/j.plaphy.2025.109592","DOIUrl":"10.1016/j.plaphy.2025.109592","url":null,"abstract":"","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":" ","pages":"109592"},"PeriodicalIF":6.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143409956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNAi-mediated down-regulation of the endogenous GhAIP10.1 and GhAIP10.2 genes in transgenic cotton (Gossypium hirsutum) enhances the earliness and yield of flower buds","authors":"Marcos Fernando Basso ,&nbsp;Thuanne Pires Ribeiro ,&nbsp;Isabela Tristan Lourenço-Tessutti ,&nbsp;Nelson Geraldo Oliveira ,&nbsp;Reneida Aparecida Godinho Mendes ,&nbsp;Niday Alline Nunes Fernandes ,&nbsp;Fabricio Barbosa Monteiro Arraes ,&nbsp;Carolina Vianna Morgante ,&nbsp;Adriana Silva Hemerly ,&nbsp;Maria Fatima Grossi-de-Sa","doi":"10.1016/j.plaphy.2025.109937","DOIUrl":"10.1016/j.plaphy.2025.109937","url":null,"abstract":"<div><div>Armadillo BTB Arabidopsis protein 1 (AtABAP1) plays a central role in the cell cycle. ABAP1-interacting protein 10 (AtAIP10, a Snf1 kinase interactor-like protein) is a protein that interacts with AtABAP1. Down-regulation of the <em>AtAIP10</em> gene in <em>A. thaliana</em> resulted in an altered cell cycle and increased photosynthesis, chlorophyll content, metabolites, plant growth, root system, seed yield, and drought tolerance. Herein, aimed to test whether the down-regulation of <em>GhAIP10</em> genes can stimulate the cotton plants in a manner similar to those observed in <em>A. thaliana</em>. Cotton transgenic events containing transgenes carrying RNA interfering (RNAi) or artificial miRNA (amiRNA) strategies were successfully generated to down-regulate the endogenous <em>GhAIP10.1</em> and <em>GhAIP10.2</em> genes. From these 15 transgenic events, five RNAi-based transgenic lines and five amiRNA-based transgenic events were selected for further analyses. The down-regulation of the <em>GhAIP10.1</em> and <em>GhAIP10.2</em> genes was confirmed by real-time RT-PCR. Phenotypic and physiological analyses revealed that these transgenic lines exhibited earlier production and opening of flower buds, increased vegetative growth over time and root biomass, no reduction in susceptibility to root-knot nematodes, and improved drought tolerance indicated by a higher photosynthetic rate and better intrinsic water-use efficiency. Based on the high identity of amino acid sequences, motifs, domains, subcellular localization, tertiary structure, down-regulation of <em>GhABAP1</em> (partner of GhAIP10), up-regulation of <em>GhCdt1</em> (a marker of the ABAP1 network), up-regulation of <em>GhCyclinB1</em> (a marker of the cell cycle), up-regulation of <em>GhAP3</em> (involved in vegetative to reproductive transition), and the up-regulation of <em>CAB3</em>, <em>NDA1</em>, <em>DJC22</em>, and <em>DNAJ11</em> genes (involved in plant resilience) suggested that GhAIP10.1 and GhAIP10.2 proteins may act in cotton similarly to the AtAIP10 protein in <em>A. thaliana</em>. Furthermore, <em>GhAIP10.1</em> and <em>GhAIP10.2</em> genes are suggested as biotechnological targets for cotton genetic engineering based on genome editing.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"225 ","pages":"Article 109937"},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GIS mediates GA signaling to directly target the expression of SPL15 to regulate trichome development in Arabidopsis thaliana","authors":"Chunyan Yang ,&nbsp;Lili Sun ,&nbsp;Muhammad Umair Yasin ,&nbsp;Haider Zulqarnain ,&nbsp;Bahar Ali ,&nbsp;Yihua Liu ,&nbsp;Bohan Liu ,&nbsp;Yinbo Gan","doi":"10.1016/j.plaphy.2025.109975","DOIUrl":"10.1016/j.plaphy.2025.109975","url":null,"abstract":"<div><div>Trichome development in <em>Arabidopsis thaliana</em> is regulated by a complex network of genetic and hormonal signaling pathways. GA/DELLA/GISs and miR156/SPLs are the two main regulatory modules regulating trichome initiation in <em>A. thaliana</em>. However, it is not clear whether there is an interaction between them. Furthermore, <em>GIS</em> was the first gene in the <em>GIS</em> family to be identified as regulating trichome development through GA signal in <em>A. thaliana</em>. Nevertheless, how GIS regulates trichome development through GA signal is still unknown. In this study, we first illuminated that GIS could directly target the expression of <em>SPL15</em> to regulate trichome development through GA signal. Our results showed that the loss of <em>SPL15</em> function significantly increases trichome density, whereas its overexpression suppresses trichome formation. Moreover, exogenous GA treatment promotes trichome development in the <em>SPL15</em> overexpressing lines. More importantly, molecular and genetic studies revealed that GIS acts upstream of <em>SPL1</em>5 by binding to its promoter to regulate the trichome development in <em>A. thaliana</em>. These indicate that C2H2 transcriptional factor <em>GIS</em> may be the key bridge gene connecting miR156/SPLs and GA/DELLA/GIS modules to regulate trichome development in <em>A. thaliana.</em> These results reveal a novel pathway, GA-GIS-<em>SPL15</em>, to regulate trichome development through the GA pathway and extend our knowledge of the GA-GIS-<em>SPL15</em> regulating network.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"225 ","pages":"Article 109975"},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Onion AcGSTU27 participated in S-alk(en)ylcysteine sulfoxides (CSOs) biosynthesis to improve abiotic stress tolerance","authors":"Lei Qin ,&nbsp;Xu Zhang ,&nbsp;Du Liu ,&nbsp;Lu Tian ,&nbsp;Guangyu He ,&nbsp;Yumeng Pang ,&nbsp;Tingting Gao ,&nbsp;Yong Wang","doi":"10.1016/j.plaphy.2025.109974","DOIUrl":"10.1016/j.plaphy.2025.109974","url":null,"abstract":"<div><div>Glutathione S-transferases (GSTs) serve as multifunctional proteins involved in diverse catalysis and regulatory processes. GSTs catalyze the conjugation of glutathione (GSH) bound to an exogenous substrate and are involved in plant stress response. S-alk(en)ylcysteine sulfoxides (CSOs) are the main bioactive substances of onion. GSTs are potentially responsible for the conversion of GSH into S-(2-carboxypropyl) glutathione, an intermediate in the biosynthesis of CSOs. Here, a GST gene, <em>AcGSTU27</em>, was determined to be participating in CSOs biosynthesis in onion. <em>AcGSTU27</em> overexpression (OE) increased the CSOs biosynthesis in onion callus. Exogenous GSH promoted the accumulation of CSOs, especially in <em>AcGSTU27</em>-OE lines. Furthermore, abiotic stress promoted CSOs accumulation in onion. Gene expression analysis revealed that <em>AcGSTU27</em> was significantly up-regulated under abiotic treatment. The CSOs contents of <em>AcGSTU27-</em>OE and wild-type (WT) callus were significantly increased under Cu, Cd, and glufosinate treatments. Moreover, the increase in CSOs levels in <em>AcGSTU27</em>-OE materials was higher than that in the WT. The results reveal that <em>AcGSTU27</em> participated in CSOs biosynthesis and can respond to abiotic stresses. Ectopic overexpression <em>AcGSTU27</em> in <em>Arabidopsis</em> promoted the heavy-metal and herbicide tolerance of transgenic lines. <em>AcGSTU27</em> transgenic plants showed better adaptability to Cu, Cd, and glufosinate treatment with a lower malondialdehyde content and higher antioxidant enzyme activities. Overall, these findings suggest that <em>AcGSTU27</em> participated in CSOs biosynthesis under abiotic stress in onion. <em>AcGSTU27</em> also improved plant stress tolerance through antioxidant activity regulation. This work enhances our comprehension of the biosynthetic mechanisms underlying CSOs in onion.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109974"},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DsGGCT2-1 involved in the detoxification of Cd and Pb through the glutathione catabolism in Dianthus spiculifolius","authors":"Binbin Liu ,&nbsp;Qi Wang ,&nbsp;Jing Guan ,&nbsp;Shufang Gong ,&nbsp;Tuanyao Chai ,&nbsp;Jingang Wang ,&nbsp;Kun Qiao","doi":"10.1016/j.plaphy.2025.109976","DOIUrl":"10.1016/j.plaphy.2025.109976","url":null,"abstract":"<div><div>Toxic heavy metals seriously affect plant growth and human health. Among the heavy metals, cadmium (Cd) and lead (Pb) are serious pollutants. <em>Dianthus spiculifolius</em> has strong tolerance to, and an ability to accumulate, heavy metals. Therefore, it has potential applications as a heavy metal hyperaccumulator. Gamma glutamylcyclotransferase (GGCT) is a key enzyme in maintaining glutathione homeostasis, and it plays a role in plant growth and development and in responses to various stresses. Previously, <em>DsGGCT2-1</em> was identified as a gene showing significantly increased transcript levels in response to Cd and Pb by transcriptome analysis. In this study, DsGGCT2-1 was confirmed to increase the Cd and Pb tolerance of transgenic yeast, <em>Arabidopsis,</em> and <em>Dianthus</em>, decrease the their accumulation in <em>Dianthus</em>. Overexpression of <em>DsGGCT2-1</em> in <em>D. spiculifolius</em> plants resulted in increased GGCT activity, higher glutamate (Glu), and glutathione (GSH) content. The results suggest that more Glu is synthesized to maintain GSH homeostasis through the activity of GGCT2-1 in the glutamyl cycle, and the generated GSH is used to chelate with toxic heavy metals, and reduce the toxicity of heavy metals in the cytoplasm. These findings will be useful for devising strategies to remediate heavy metal-polluted soils, and for breeding plants that tolerate heavy metals.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"225 ","pages":"Article 109976"},"PeriodicalIF":6.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the spatiotemporal strategies of plants in response to biotic and abiotic stresses:A comprehensive review","authors":"Guo Yu ,&nbsp;Jingyu Xiang ,&nbsp;Caixing Lai ,&nbsp;Xiaoming Li ,&nbsp;Geoffrey I. Sunahara ,&nbsp;Fujin Mo ,&nbsp;Xuehong Zhang ,&nbsp;Jie Liu ,&nbsp;Hua Lin ,&nbsp;Gang Liu","doi":"10.1016/j.plaphy.2025.109967","DOIUrl":"10.1016/j.plaphy.2025.109967","url":null,"abstract":"<div><div>Plant functions are governed by complex regulatory mechanisms that operate across diverse cell types in various tissues. However, the challenge of dissecting plant tissues has hindered the widespread application of single-cell technologies in plant research. Recent advancements in single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) have propelled the field forward. scRNA-seq enables the examination of gene expression at the single-cell level, while ST preserves the spatial context of cellular organization. While previous reviews have discussed the breakthroughs of scRNA-seq and ST in plants, none have comprehensively addressed the use of these technologies to study plant responses to environmental stress at the cellular level. This review provides an in-depth analysis of the development, advantages, and limitations of single-cell and spatial transcriptomics, highlighting their critical role in unraveling plant strategies for coping with biotic and abiotic stresses. We also explore the challenges and future prospects of integrating scRNA-seq and ST in plant research. Understanding cell-specific responses and the complex interactions between cellular entities within the plant under stress is essential for advancing our knowledge of plant biology.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109967"},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel auxin methyltransferase of the SABATH family for phenylacetic acid methylation is conserved in potato and tomato","authors":"Weijiao Wang ,&nbsp;Chi Zhang ,&nbsp;Hong Guo ,&nbsp;Feng Chen","doi":"10.1016/j.plaphy.2025.109972","DOIUrl":"10.1016/j.plaphy.2025.109972","url":null,"abstract":"<div><div>The SABATH family of methyltransferases is known for methylating a wide range of substrates, including hormones and secondary metabolites. A notable member of this family is the auxin methyltransferase IAMT which uses indole-3-acetic acid (IAA) as the substrate. This study aims to determine whether methyl phenylacetate (MePAA), the methyl ester of another auxin, phenylacetic acid (PAA), is synthesized by SABATH methyltransferases. Potato (<em>Solanum tuberosum</em> L. cv. Désirée) was chosen as the primary model because it produces MePAA exclusively in flowers. Based on the structural similarity of IAA and PAA, our initial hypothesis was that MePAA is synthesized by an IAMT-like enzyme. The potato genome contains two <em>IAMT-like</em> genes. However, their recombinant enzymes expressed in <em>Escherichia coli</em> were shown to catalyze the methylation of IAA but not PAA, thus rejecting our initial hypothesis. Among the 23 potato <em>SABATH</em> genes, two exhibited flower-specific expression. One was excluded because it had already been identified as an IAMT. <em>In vitro</em> assays of the enzyme encoded by the other gene, <em>StSABATH6</em>, confirmed its catalytic activity against PAA. Consequently, this enzyme was renamed StPAAMT. Notably, StPAAMT has an ortholog in both cultivated and wild tomatoes. The gene from tomato (<em>Solanum lycopersicum</em>), <em>SlPAAMT</em>, was verified to encode PAA methyltransferase. Further genomic and phylogenetic analyses of five <em>Solanum</em> species showed that the <em>PAAMT</em> gene was likely absent in eggplant (<em>Solanum melongena</em>), implying its origin in the common ancestor of potato and tomato. The structural analysis identified key amino acids associated with the substrate specificity of PAAMT. This work provides new insights into the evolution of auxin methyltransferases, particularly PAAMT, as members of the SABATH family.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109972"},"PeriodicalIF":6.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}