Plant Physiology and Biochemistry最新文献

{"title":"Uncovering the crucial metabolic pathways in the invasive plant Hydrocotyle verticillata for defence against copper exposure by integrative transcriptome and metabolome analysis","authors":"Jianpan Xin,&nbsp;Chu Zhao ,&nbsp;Yan Li,&nbsp;Wenke Ji,&nbsp;Runan Tian","doi":"10.1016/j.plaphy.2025.109814","DOIUrl":"10.1016/j.plaphy.2025.109814","url":null,"abstract":"<div><div>To throw light on the underlying strategies of <em>Hydrocotyle verticillata</em> coping with potentially toxic elements (PTEs) exposure. In this study, variations in the transcriptome and metabolomics of the leaves of plants exposed to various concentrations of copper ions (Cu²⁺) for 7 d were analyzed. Several crucial metabolic pathways involved in leaf defense against Cu²⁺ exposure were identified using integrative transcriptome and metabolome analysis. The pathways for plants coping with Cu²⁺ exposure were associated with carotenoid metabolism, amino acid metabolism, cutin, suberin, and wax biosynthesis, plant hormonal signal transduction, phenylpropanoid and terpenoid metabolisms. In the 90.0 μM Cu²⁺ treatment, abscisic acid 8′-hydroxylase was upregulated, reducing abscisic acid content, while downregulation of the BAK1 gene and pathogen-related protein genes triggered programmed cell death The positive role of antheraxanthin, γ-L-glutamylcysteine, γ-aminobutyric acid, and carnosine in the plant defense against 45.0 μM Cu²⁺ was observed. Lutein, 3,4-dihydrospheroidene, linoleic acid, glutamine, pyroglutamic acid, the gene encoding brassinosteroid resistant 1/2, and xyloglucan:xyloglucosyl transferase are involved in plant defense against 90.0 μM Cu²⁺. Compared to 0 mM, both Cu²⁺ treatments upregulated hexadecanedioic acid abundance and the gene encoding the auxin response protein. This study provides new insights into the underlying mechanisms through which invasive plants defend against PTE exposure.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109814"},"PeriodicalIF":6.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between OsLEC1 and OsHDA710 positively regulates callus formation in rice","authors":"Wenzhuo Zeng ,&nbsp;Jingjing Li ,&nbsp;Dandan Li ,&nbsp;Jinhan Lu ,&nbsp;Yidan Pan ,&nbsp;Haonan Wei ,&nbsp;Yangfan Chen ,&nbsp;Qingyao Shu ,&nbsp;Hongwu Bian ,&nbsp;Fu Guo","doi":"10.1016/j.plaphy.2025.109826","DOIUrl":"10.1016/j.plaphy.2025.109826","url":null,"abstract":"<div><div>Transgenic and gene editing technologies often begin with callus induction through embryo in vitro culture in rice. Mutations in both <em>OsLEC1</em> (the rice homolog of <em>Leafy Cotyledon 1</em>) and <em>OsHDA710</em> (encoding a deacetylase) can severely impair callus induction in embryo culture. However, whether these genes are part of a common regulatory module remains unclear. This study demonstrates defective scutellum-derived callus induction in novel OsLEC1 knockout (<em>Oslec1</em>) lines, indicating its crucial role in this process. Immunoprecipitation-mass spectrometry assays revealed that OsHDA710 interacts with OsLEC1 in embryo-derived callus. Yeast two-hybrid and bimolecular fluorescence complementation assays confirmed that OsLEC1 interacts with OsHDA710 via its C-terminal domain. Comparative transcriptome analyses revealed a set of shared genes that were upregulated in scutellum-derived callus of both <em>Oslec1</em> and <em>Oshda710</em> mutants. The upregulated genes included the nuclear factor Y family transcription factors, as well as genes involved in hormone biosynthesis and signaling, starch and lipid synthesis and metabolism, and abiotic stress responses. Our study indicates that OsLEC1 and OsHDA710 collaborate to regulate callus induction in rice through a common pathway.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109826"},"PeriodicalIF":6.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143713817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin-mediated adaptation of spinach (Spinacia oleracea L.) in acidic soil","authors":"Md.Kamrul Hasan ,&nbsp;Md. Abdullah Al-Hasib Imon ,&nbsp;Israt Jahan ,&nbsp;Tushar Kanti Das ,&nbsp;Miskat Shukrana Mishu ,&nbsp;Jannat Shopan ,&nbsp;Munmun Saha ,&nbsp;Md. Sirajul Islam Khan ,&nbsp;Md. Shariful Islam ,&nbsp;Golam Jalal Ahammed","doi":"10.1016/j.plaphy.2025.109816","DOIUrl":"10.1016/j.plaphy.2025.109816","url":null,"abstract":"<div><div>Soil acidity is a widespread and critical factor contributing to soil degradation. Melatonin (MT) as a protective biostimulant, plays a crucial role in plant growth under harsh environments. However, the role of MT in plant adaptation to acidic soil remains elusive. Hence, here we tested the effects of exogenous MT on the adaptation of spinach <strong>(</strong><em>Spinacia oleracea</em> L.) crop to acidic soil (pH 4.0). Initially, a series of MT concentrations (0, 25, 50, 100, and 200 μM) were tested to evaluate the seed germination and growth attributes of spinach under low pH levels. Among the doses, 100 μM MT showed better results in terms of germination percentage and biomass accumulation, which was then selected for further experiments. Results showed that exogenous MT significantly increased plant adaptation to acidic soil as evidenced by increased photosynthesis rate and biomass accumulation with enhanced total antioxidant capacity, DPPH free radical scavenging, phenol, flavonoid, and glutathione contents compared with soil acidity treatment alone. Moreover, MT alleviated oxidative stress indices such as electrolyte leakage and malondialdehyde in acid-stressed plants, suggesting an effective strategy of adaptation. Furthermore, MT-treated plants exhibited higher macro and micronutrient accumulation along with higher vitamin B complex, vitamin C, and protein content, leading to an increased nutritional quality and yield compared to control. Therefore, the study concludes that the application of MT at 100 μM could be a sustainable alternative approach to adapt spinach farming in acidic soil.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109816"},"PeriodicalIF":6.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated metabolomic and transcriptomic analyses reveal anthocyanin biosynthesis mechanisms and the regulatory role of LjAN2 in Lonicera japonica","authors":"Zhengwei Tan ,&nbsp;Dandan Lu ,&nbsp;Lei Li ,&nbsp;Yongliang Yu ,&nbsp;Xiaoyu Su ,&nbsp;Yao Sun ,&nbsp;Yiwen Cao ,&nbsp;Chunming Li ,&nbsp;Wei Dong ,&nbsp;Hongqi Yang ,&nbsp;Qing Yang ,&nbsp;Sufang An ,&nbsp;Huizhen Liang","doi":"10.1016/j.plaphy.2025.109824","DOIUrl":"10.1016/j.plaphy.2025.109824","url":null,"abstract":"<div><div><em>Lonicera japonica</em> flowers are a very commonly used traditional Chinese herb. Anthocyanins are the source of flower pigments, and also are renowned for their therapeutic activities. However, the specific anthocyanin composition and regulatory mechanisms governing their accumulation in <em>L. japonica</em> varieties remain unclear. Here, we first investigated the changes in flower color and anthocyanin content during development in the green flower (GFLJ) and the purple flower (PFLJ) cultivars of <em>L</em>. <em>japonica</em>. Results show GFLJ has green flowers and PFLJ has purple flowers, which are especially remarkable during S3-S4 stages. Accordingly, PFLJ had much higher (&gt;10 times) anthocyanins contents at all the six flower stages than those of GFLJ. Further metabolomic analysis in S3 stage flowers found that most anthocyanins showed increased accumulation, whereas flavones and flavonols showed decreased accumulation in PFLJ compared to GFLJ. Transcriptome analysis identified 21 (85.7 % upregulated) anthocyanin synthase gene DEGs, and 23 MYB transcription factor (TF) DEGs (play essential roles in regulating anthocyanin biosynthesis). In addition, 19 GST and 14 MATE DEGs (play key roles in anthocyanins accumulation) were identified. Further, we found a novel MYB TF (<em>LjAN2</em>) that showed much higher expression in PFLJ. <em>LjAN2</em> overexpression in tobacco led to purple leaves, and the upregulation of anthocyanin synthase genes (<em>NtCHS</em> and <em>NtANS</em>), as well as increased anthocyanin accumulation. This research offers a comprehensive understanding of the molecular basis of anthocyanin biosynthesis in <em>L. japonica</em>, highlighting its potential applications in the pharmaceutical industry.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109824"},"PeriodicalIF":6.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foliar exposure to microplastics disrupts lettuce metabolism and negatively interferes with symbiotic microbial communities","authors":"Ruiying Shi ,&nbsp;Yuhang Lian ,&nbsp;Aurang Zeb ,&nbsp;Jinzheng Liu ,&nbsp;Miao Yu ,&nbsp;Qi Wang ,&nbsp;Jianling Wang ,&nbsp;Xiuping Fu ,&nbsp;Weitao Liu","doi":"10.1016/j.plaphy.2025.109823","DOIUrl":"10.1016/j.plaphy.2025.109823","url":null,"abstract":"<div><div>Plant leaves are considered an important sink for atmospheric microplastics (MPs) because they serve as a vital interface between the atmosphere and terrestrial ecosystems. However, there is still a dearth of information regarding how plant-symbiotic microbe-soil systems are affected by foliar exposure to MPs. In this study, MPs (polystyrene (PS), polyethylene (PE), and polypropylene (PP)) were sprayed over soil-cultivated lettuce (<em>Lactuca sativa</em> L.) four occasions, with final sprays containing 0.4 and 4 μg of MPs per plant. MPs had no discernible impact on lettuce growth as compared to the control group. However, MPs led to reductions in relative chlorophyll content from 16.91 to 30.64 % and net photosynthetic rate from 6.64 to 81.41 %. These results validate the phytotoxicity linked to MP exposure through foliar application. The presence of MPs triggered interspecific competition among phyllosphere microbial species and reduced microbial network complexity by forming ecological niches and regulating carbon- and nitrogen-related metabolic pathways. Furthermore, MPs inhibited the growth of beneficial bacteria in the rhizosphere soil, including a variety of plant growth-promoting bacteria (PGPR) such as <em>Rhizobiales</em>, <em>Pseudomonadales</em>, and <em>Bacillales</em>. This study identifies the ecological health risks associated with atmospheric MPs, which may have a detrimental impact on crop production and further compromise soil ecosystem security.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109823"},"PeriodicalIF":6.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transport properties and accumulation patterns of trivalent chromium in rice: A hydroponic and modeling approach","authors":"Weipeng Xie ,&nbsp;Jieping Yu ,&nbsp;Daye Luo ,&nbsp;Ran Wei ,&nbsp;Ting Liu ,&nbsp;Shengsheng Sun ,&nbsp;Jingjing Li ,&nbsp;Fujun Wang ,&nbsp;Chao Jin ,&nbsp;Yetao Tang ,&nbsp;Ruiying Du ,&nbsp;Tenghaobo Deng ,&nbsp;Rongliang Qiu","doi":"10.1016/j.plaphy.2025.109825","DOIUrl":"10.1016/j.plaphy.2025.109825","url":null,"abstract":"<div><div>Although rice is the staple food of the majority of the human population worldwide, rice consumption is a significant pathway of human exposure to chromium (Cr). However, the primary pathways and periods of accumulation of Cr(III), the main form of Cr in rice, remain unexplored. Hence, in this study, we conducted experiments involving foliar application of Cr(III) and hydroponic Cr(III) supplementation at various growth stages of rice. The results showed that the phloem translocation rate of Cr(III) was lower than that of strontium, which is a phloem-immobile element. The Cr(III) concentration in leaf phloem sap drastically decreased soon after Cr(III) supply was stopped in hydroponic culture solutions, indicating rapid sequestration and compartmentalization of Cr(III) in the leaves. The filling and heading stages were identified as the critical Cr(III) accumulation periods for rice grains and whole rice plants, respectively. According to our model calculations, 80 % of Cr(III) that accumulated in rice grains is transported via the xylem, while the remaining 20 % is remobilized from nutrient tissue. Moreover, Cr(III) and Fe(III) exhibited similar remobilization and accumulation patterns in various parts of rice plants. These findings highlight the importance of implementing soil Cr immobilization during the filling stage to ensure the safe production of rice.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109825"},"PeriodicalIF":6.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An extremely acidic environment microalga from Tinto River as a novel source of anti-inflammatory activity","authors":"María Robles ,&nbsp;Carla S.B. Viegas ,&nbsp;Rafael Torronteras ,&nbsp;Inés Garbayo ,&nbsp;José M. Vega ,&nbsp;Dina C. Simes ,&nbsp;Carlos Vílchez","doi":"10.1016/j.plaphy.2025.109815","DOIUrl":"10.1016/j.plaphy.2025.109815","url":null,"abstract":"<div><div><em>Coccomyxa onubensis</em> (<em>C. onubensis</em>) is an acidotolerant microalga isolated from the extremely acidic Tinto River (Huelva), which contains high levels of metals in solution, mainly Fe and Cu. This makes <em>C. onubensis</em> an adequate candidate to easily cope with high levels of oxidative stress by increasing the levels of certain molecules and enzymes, which helps them elicit an adequate antioxidant biochemical response. Thus, <em>C. onubensis</em> is a promising source of bioactive compounds which exhibit <em>in vitro</em> anti-inflammatory activity, including fatty acids, (poly)phenolic compounds and carotenoids. In this study, the correlations between the antioxidant response and anti-inflammatory activity of cell extracts obtained from Fe (III)-stressed microalgal cultures were analyzed. The results suggested a direct relationship between the antioxidant capacity of the microalgal extracts and Fe (III) concentration in the culture medium. Consequently, the production of some of the target antioxidant molecules, including carotenes, xanthophylls and (poly)phenols, increased. The levels of these molecules increased the most in cell extracts obtained from microalgal cultures at 0.25 mM of Fe (III), which was correlated with a 50 % increase in the anti-inflammatory activity of the microalgal extracts in THP-1 differentiated human macrophages. Fe (III)-modulated oxidative stress allowed us to define culture conditions that can enhance the anti-inflammatory activity of <em>C. onubensis</em> extracts, which are enriched in valuable antioxidant molecules. Overall, this study highlighted the utility of a microalgal species from a highly acidic environment as a novel, natural source of anti-inflammatory agents, based on its ability to cope with the oxidative conditions of its habitat.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109815"},"PeriodicalIF":6.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silicon priming triggers differential physiological, ionomic and metabolic responses in olive (Olea europaea L.) cultivars with different tolerance to salinity","authors":"Carmen Fidalgo-Illesca,&nbsp;Alessandra Francini,&nbsp;Andrea Raffaelli,&nbsp;Luca Sebastiani","doi":"10.1016/j.plaphy.2025.109809","DOIUrl":"10.1016/j.plaphy.2025.109809","url":null,"abstract":"<div><div>Salinity stress can negatively influence the growth potential and productivity of olive trees affecting photosynthesis and, disturbing ions homeostasis and essential metabolic pathways. Silicon (Si) is proposed as exogenous pretreatment for mitigate the salinity impact on olive plants. One-year old ‘Frantoio’ (salt-tolerant) and ‘Leccino’ (salt-sensitive) plants (n = 5) were grown in pots filled with sand and clay and pretreated for 28 days with 10 mg L⁻¹ of Si(OH)₄ then, for 51 days with 100 mM NaCl (12.15 g for each plant) and compared to control plants. The following hypotheses have been tested: i) Si pretreatment enhances photosynthetic performance by regulating stomatal closure and decreasing water loss; ii) Si reduces Na⁺ uptake and accumulation in new leaves; iii) Si improve the biosynthesis of compatible osmolytes that have a role in the regulation of the osmotic stress induced by salinity. The Si priming effect in olive tree was cultivar dependent. In ‘Frantoio’ Si induce a rapid early decrease of stomatal conductance increasing the intrinsic water use efficiencies (<em>int</em>WUE) not observed in ‘Leccino’ plants. In ‘Leccino’ the key Si effect was the reduction of Na⁺ accumulation in new leaves (−58 %) and maintenance of the K⁺ concentration under salinity. Specific interactions between Si and NaCl and the number of polyphenols affected were higher in ‘Frantoio’ than in ‘Leccino’. Among the key mechanisms related to the Si-mediated tolerance to salt stress we can conclude that photosynthesis and Na⁺ uptake are the two principals involved in the responses of salinity to ‘Frantoio’ and ‘Leccino’ cultivars.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109809"},"PeriodicalIF":6.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated metabolome and transcriptome analyses revealed key cytochrome P450 genes involved in the biosynthesis of oleanane-type saponins in Hedera helix L.","authors":"Yuedong He ,&nbsp;Jing Zhang ,&nbsp;Yuewei He ,&nbsp;Yun Tian ,&nbsp;Huhu Liu ,&nbsp;Chong Wang ,&nbsp;Guiping Guan ,&nbsp;Xiangyang Lu ,&nbsp;Lei Yuan ,&nbsp;Wei Xiang ,&nbsp;Jianguo Zeng ,&nbsp;Xiaohong Zhong","doi":"10.1016/j.plaphy.2025.109818","DOIUrl":"10.1016/j.plaphy.2025.109818","url":null,"abstract":"<div><div><em>Hedera helix</em> L. is a traditional Chinese medicinal and industrial crop commonly used to treat coughs and upper respiratory tract diseases. Additionally, it can be utilized as insecticidal, mosquito repellent and biopesticide. Its primary components are pentacyclic triterpenoid saponins include oleanolic acid, hederagenin, hederacoside C, etc. Currently, cytochrome P450 (CYP450) has been shown to be closely associated with the structural diversification and functional modification of the triterpenoid. However, the research on <em>H. helix</em> is still shallow, especially the functional characterization of CYP450 gene in the stage of modifying pentacyclic triterpenoid skeleton. This study integrated analyzed transcriptome and the accumulation modes of the main metabolites of <em>H. helix</em> and screened six CYP450 candidate genes. RT-qPCR results showed that candidate genes exhibited tissue specificity and inducible expression specificity. Based on <em>in vitro</em> and <em>in vivo</em> validation, both <em>HhCYP716A409</em> and <em>HhCYP716S11</em> showed activity of oxidase in β-amyrin C-28, producing oleanolic acid by participating in the C-28 oxidization of β-amyrin. <em>HhCYP72D57</em>, <em>HhCYP72A1140</em>, and <em>HhCYP72A1141</em> produced hederagenin by participating in the hydroxylation of oleanolic acid C-23. Additionally, <em>HhCYP72D57</em>, <em>HhCYP72A1139</em>, and <em>HhCYP72A1141</em> were also involved in the hydroxylation of hederagenin C-16 to produce 16-OH hederagenin. This study confirms the pivotal roles of CYP716 and CYP72 families in oleanane-type triterpenoid biosynthesis and establishes a method to efficiently produce hederacoside C and derivatives, providing a genetic toolkit for metabolic engineering of <em>H. helix</em> to scale saponin production for pharmaceuticals, agrochemicals, or synthetic biology-driven design of novel triterpenoid biopesticides.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109818"},"PeriodicalIF":6.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordination of plant functional traits under nitrogen deposition with phosphorus addition in a desert steppe ecosystem","authors":"Leren Liu ,&nbsp;Yifan Xia ,&nbsp;Kuo Sun ,&nbsp;Ruojun Sun ,&nbsp;Zhenzhu Xu ,&nbsp;Feng Zhang","doi":"10.1016/j.plaphy.2025.109813","DOIUrl":"10.1016/j.plaphy.2025.109813","url":null,"abstract":"<div><div>Understanding how plant functional traits respond to nutrient enrichment becomes more crucial for predicting changes in grassland community composition and functions under global changes. However, it remains unclear how nitrogen (N) and phosphorus (P) additions jointly influence a variety of leaf traits and how they coordinate with contrastingly adaptive mechanisms in arid ecosystems. A two-year field experiment with five N levels and two P treatments was conducted to examine the effects of N and P additions on leaf/community functional traits in a desert steppe. We found N addition significantly affected the other six leaf morphological and nutrient traits except leaf thickness (LT); nitrogen addition remarkably increased leaf nitrogen concentration (N_mass) and decreased C/N with or without P; nitrogen addition profoundly elevated stomatal conductance (<em>g</em>_s) but did not obviously affect photosynthetic rate (<em>A</em>_area) except <em>Tribulus terrestris</em>. Compared to grasses, the annual forb <em>T. terrestris</em> exhibited stronger competitiveness (N_mass, <em>A</em>_area) with increased N application. Nitrogen addition obviously increased community-weighted means (CWMs) of N_mass, specific leaf area (SLA), plant height, <em>g</em>_s and <em>A</em>_area, improving aboveground biomass (AGB), whereas P addition significantly enhanced CWM of SLA but reduced CWMs of transpiration rate and LT. With increasing N addition rates, dominant S-strategy species (higher LT and C/N) in low-nutrient environments were gradually substituted by R-strategy species (higher N_mass and <em>A</em>_area) in high-nutrient environments. Our results highlight differential responses of plant functional traits to nutrient enrichment and divergent adaptive strategies among species should be considered when assessing the impacts of global environmental changes on community assembly and functioning.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109813"},"PeriodicalIF":6.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}