Plant Physiology and Biochemistry最新文献

{"title":"ZmNAC20 positively regulates drought tolerance in maize seedlings via a chromatin-associated network","authors":"Zhongyu Wang,&nbsp;Yating He,&nbsp;Yang Yang,&nbsp;Ning Li,&nbsp;Changyu Li","doi":"10.1016/j.plaphy.2026.111800","DOIUrl":"10.1016/j.plaphy.2026.111800","url":null,"abstract":"<div><div>The potential yield and establishment of maize are severely restricted by early developmental drought. Although NAC transcription factors are known to control abiotic stress responses, the role of NAC transcription factor 20 (<em>ZmNAC20</em>) in maize drought adaptation remains unclear. We characterized the function of <em>ZmNAC20</em> in maize seedlings using CRISPR/Cas9 mutagenesis combined with molecular, physiological, and multi-omics approaches. Loss-of-function <em>zmnac20</em> mutants displayed increased sensitivity to drought stress, indicating that <em>ZmNAC20</em> positively regulates drought tolerance in maize seedlings. Further analyses revealed that <em>NAC transcription factor 110</em> (<em>ZmNAC110</em>) functions upstream of <em>ZmNAC20</em>, activating its transcription and physically interacting with the ZmNAC20 protein. CUT&amp;Tag profiling indicated that <em>ZmNAC20</em> is associated with alterations in H3K27me3 distribution across active genomic regions. By integrating CUT&amp;Tag-seq, AI-based protein-protein interaction predictions, and RNA-seq datasets, we identified candidate downstream components associated with <em>ZmNAC20</em>. Among these, <em>YIGE1</em> was the most strongly supported candidate. <em>ZmNAC20</em> activated <em>YIGE1</em> promoter activity and interacted with the YIGE1 protein. Together, these results identify <em>ZmNAC20</em> as a positive regulator of drought tolerance in maize seedlings and identify a candidate <em>ZmNAC110</em>-<em>ZmNAC20</em>-<em>YIGE1</em> regulatory module linked to chromatin-associated drought responses. Because the genetic requirement for <em>YIGE1</em> was not tested, <em>YIGE1</em> is presented here as a strong candidate downstream component rather than a confirmed one.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"239 ","pages":"Article 111800"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR-Cas9 knockout of IBH1 fine-tunes brassinosteroid metabolism and modulates plant architecture and grain traits in durum wheat","authors":"Chiara D'Attilia,&nbsp;Marco Bonarrigo,&nbsp;Danuše Tarkowská,&nbsp;Valentina Buffagni,&nbsp;Sadiye Hayta,&nbsp;Mark A. Smedley,&nbsp;Stefania Masci,&nbsp;Samuela Palombieri,&nbsp;Francesco Sestili","doi":"10.1016/j.plaphy.2026.111831","DOIUrl":"10.1016/j.plaphy.2026.111831","url":null,"abstract":"<div><div>Brassinosteroids (BRs) are essential steroidal plant hormones that regulate cell elongation, vascular differentiation and reproductive development, thereby shaping plant architecture and grain yield. Fine-tuning BR signalling, rather than altering hormone accumulation, has been proposed as a potential strategy to regulate plant development.</div><div>Here we investigated the role of IBH1 transcription factor, a negative regulator within the ILI1/PRE1-IBH1 module controlling BR-mediated cell elongation in durum wheat (<em>Triticum turgidum</em> ssp. <em>durum</em>), using a CRISPR-Cas9-mediated knockout approach. IBH1 loss of function was associated with transcriptional changes consistent with enhanced BR signalling, including the coordinated upregulation of key signalling components (BRI1 and BZR1) and downstream regulators (ILI1 and PRE1). Metabolic profiling revealed selective remodelling of BR biosynthesis, characterized by differential accumulation of specific pathway intermediates rather than a general increase in BR content. Phenotypic analyses showed that <em>TdIBH1</em> mutants exhibited increased plant height, internode elongation, leaf inclination, grain size, and thousand grain weight, while no significant differences in fertility were detected. These findings support a role for TdIBH1 as a conserved negative regulator of BR-mediated growth in durum wheat and suggest that the manipulation of the ILI1/PRE1-IBH1 module may provide a potential strategy to modulate plant architecture and grain-related traits in cereal crops.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"239 ","pages":"Article 111831"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Full-length transcriptome sequencing in garlic identifies AsMYB4 as a candidate regulator of flavonoid metabolism and male sterility","authors":"Jie Ge,&nbsp;Jide Fan,&nbsp;Yongqiang Zhao,&nbsp;Xinjuan Lu,&nbsp;Canyu Liu,&nbsp;Biwei Zhang,&nbsp;Qingqing Yang,&nbsp;Mengqian Li,&nbsp;Guangyang Liu,&nbsp;Xiaohui Song,&nbsp;Yi Feng,&nbsp;Yan Yang,&nbsp;Feng Yang","doi":"10.1016/j.plaphy.2026.111804","DOIUrl":"10.1016/j.plaphy.2026.111804","url":null,"abstract":"<div><div>Garlic (<em>Allium sativum</em> L.) is crop of substantial economic value cultivated globally, yet the molecular basis underlying its reproductive development remains poorly understood. Here, we generated a comprehensive full-length garlic transcriptome using PacBio single-molecule real-time sequencing and identified 13,260 high-quality isoforms. Using this resource, we characterized <em>AsMYB4</em>, an R2R3-MYB transcription factor. Heterologous overexpression of <em>AsMYB4</em> in <em>Arabidopsis thaliana</em> repressed flavonoid biosynthesis and impaired filament elongation, reduced pollen viability, and decreased fertility without detectably affecting female reproductive function. In stamens, <em>AsMYB4</em> overexpression suppressed phenylpropanoid-flavonoid metabolism and altered auxin accumulation and signaling, supporting an association between secondary metabolism and hormonal regulation during male reproductive development. Together, these findings expand the transcriptomic resources available for garlic and identify <em>AsMYB4</em> as a candidate regulator of flavonoid metabolism and male fertility.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"239 ","pages":"Article 111804"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149143703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haloxyfop-R-methyl resistance in Polypogon fugax: involvement of ABCG25 and ACCase gene overexpression","authors":"Jie Zhang,&nbsp;Haifang Lv,&nbsp;Yufei Zhao,&nbsp;Yikai Dong,&nbsp;Qinghua Jia,&nbsp;Renci Xiong,&nbsp;Sumei Wan,&nbsp;Wen Chen","doi":"10.1016/j.plaphy.2026.111835","DOIUrl":"10.1016/j.plaphy.2026.111835","url":null,"abstract":"<div><div>Asia minor bluegrass (<em>Polypogon fugax</em>) is a problematic grass weed in Chinese canola fields, and resistance to acetyl-CoA carboxylase (ACCase)-inhibiting herbicides is increasingly widespread. Here, we characterized a haloxyfop-R-methyl-resistant <em>P. fugax</em> population (CZ-R) collected from Chongzhou City, Sichuan Province. Whole-plant dose-response assays showed that the CZ-R population exhibited a 15.83-fold resistance level compared with the susceptible control CZ-S. Sequencing of the plastidic <em>ACCase</em> gene revealed no known target-site mutations, but <em>ACCase</em> transcript abundance was 5.55-fold higher in the CZ-R population. Pretreatment with the cytochrome P450 inhibitor malathion or the glutathione S-transferase inhibitor NBD-Cl did not reverse resistance, and LC-MS analysis showed no difference in haloxyfop-R-methyl residues between the two populations at 24 and 48 h after treatment. Screening of ABC transporter genes identified <em>ABCG25</em> and <em>ABCI19</em> as upregulated candidates in CZ-R, and heterologous overexpression in rice demonstrated that <em>ABCG25</em>, but not <em>ABCI19</em>, significantly enhanced haloxyfop-R-methyl tolerance. In addition, the resistant population showed lower H₂O₂ and malondialdehyde (MDA) levels and higher catalase (CAT) activity, accompanied by 7.13- and 5.61-fold upregulation of <em>CAT1</em> and <em>CAT2</em>, respectively. Collectively, these results indicate that haloxyfop-R-methyl resistance in the CZ-R population was not associated with known target-site mutations, while inhibitor and residue analyses did not support major P450- or GST-mediated degradation of the parent herbicide as the predominant resistance mechanism. Instead, resistance was associated with constitutive <em>ABCG25</em> and <em>ACCase</em> overexpression and with an enhanced antioxidant response characterized by higher CAT activity and reduced H₂O₂ and MDA accumulation. These findings support the coexistence of target-related and non-target-site resistance components and broaden our understanding of the diverse mechanisms underlying ACCase-inhibitor resistance in grass weeds.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"239 ","pages":"Article 111835"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149143704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of the NAC gene family in taro and functional analysis of CeNAC48 in starch biosynthesis","authors":"Xinbi Jia,&nbsp;Ziyin Tong,&nbsp;Xinyu Wang,&nbsp;Sikandar Amanullah,&nbsp;Yufei Tang,&nbsp;Pengcheng Zeng,&nbsp;Yao Xiao,&nbsp;Qinghong Zhou,&nbsp;Qianglong Zhu","doi":"10.1016/j.plaphy.2026.111809","DOIUrl":"10.1016/j.plaphy.2026.111809","url":null,"abstract":"<div><div>Taro (<em>Colocasia esculenta</em> (L.) Schott) is an important perennial corm crop, and its corm expansion is accompanied by starch synthesis and accumulation. NAC transcription factors (TFs) serve as important regulators of plant growth and stress adaptation. However, their regulatory mechanisms underlying corm development and starch biosynthesis in taro remain largely unknown. In this study, a total of 83 NAC genes (<em>CeNACs</em>) were identified in taro. The evolution of the <em>CeNAC</em> gene family was mainly driven by segmental duplication events followed by purifying selection. Promoter cis-element analysis indicated that most <em>CeNAC</em> genes respond to light and methyl jasmonate (MeJA) signals. Transcriptomic and qRT-PCR analyses revealed that the expression of <em>CeNAC48</em> was negatively correlated with the starch accumulation in taro. <em>CeNAC48</em> was localized in the nucleus, endoplasmic reticulum and plasma membrane, and had transcriptional activation activity. Stable overexpression of <em>CeNAC48</em> in tobacco significantly decreased starch content; consistently, overexpression of <em>CeNAC48</em> in taro calli also reduced starch content, whereas silencing of <em>CeNAC48</em> resulted in starch accumulation. Yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter (LUC) assays confirmed that <em>CeNAC48</em> directly binds to the promoters of <em>CeAGPS1</em> and <em>CeBAM</em>. By repressing <em>CeAGPS1</em> and activating <em>CeBAM</em>, <em>CeNAC48</em> negatively regulates starch synthesis in taro. These findings identify <em>CeNAC48</em> as a key conserved regulator of starch biosynthesis with cross-species application potential.This study reports the first genome-wide identification of the <em>NAC</em> gene family in taro, and provides a valuable molecular target for genetic improvement of taro quality.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"239 ","pages":"Article 111809"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early humic acid-induced eustress triggers biostimulant responses in Arabidopsis thaliana: RBOHC-derived H2O2 drives growth promotion while RBOHD enables nutritional reprogramming","authors":"Álvaro Rubio,&nbsp;Maite Olaetxea,&nbsp;Javier Erro,&nbsp;Marta Fuentes,&nbsp;Angel María Zamarreño,&nbsp;José María García-Mina","doi":"10.1016/j.plaphy.2026.111790","DOIUrl":"10.1016/j.plaphy.2026.111790","url":null,"abstract":"<div><div>Root-applied humic acids (HAs) promote plant development, yet the early signaling mechanisms underlying their biostimulant action remain incompletely understood. Here, we show that treatment of <em>Arabidopsis thaliana</em> roots with a sedimentary humic acid (SHA) triggers a transient eustress-like response, characterized by early inhibition of shoot and root growth and increased H₂O₂ accumulation in root hairs and apical roots. This initial oxidative phase was followed by enhanced shoot and root growth, supporting the view that SHA acts as an adaptive plant modulator through a controlled stress-related signal. Pharmacological H₂O₂ scavenging and genetic analysis of NADPH oxidase mutants revealed that SHA-induced growth promotion depends on RBOHC/RHD2-mediated H₂O₂ production in roots. In <em>rhd2</em> plants, SHA failed to induce the characteristic H₂O₂ increase and did not promote shoot or root growth. By contrast, <em>rbohd</em> plants retained SHA-induced growth stimulation but showed impaired activation of nutrient transporter and plasma membrane H⁺-ATPase genes, limited improvement in shoot nutrient use efficiency, and partial root architectural remodeling. These results indicate that RBOHC-derived H₂O₂ is required for the growth-promoting response, whereas RBOHD cooperates with RBOHC to engage broader nutritional and developmental programs. SHA also induced extensive hormonal reprogramming in roots and shoots; however, disruption of individual ABA, auxin, jasmonate, or cytokinin signaling components did not abolish growth promotion, suggesting partially redundant hormonal regulation downstream of ROS. Overall, our findings identify an RBOHC–H₂O₂ module as a primary entry point for SHA-mediated biostimulant action and support a model in which root-localized oxidative eustress coordinates hormonal, developmental, and nutritional reprogramming in <em>Arabidopsis</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"239 ","pages":"Article 111790"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative multi-omics reveals differential salt tolerance traits and candidate genes in domestic and foreign soybeans","authors":"Yanjuan Hu,&nbsp;Yueru Zhao,&nbsp;Haofeng Sun,&nbsp;Chang Sun,&nbsp;Meiling Liu,&nbsp;Yan Sun,&nbsp;Yiyang Wu,&nbsp;Xiaochi Liu,&nbsp;Juan E. Rosas,&nbsp;Yongzhe Gu,&nbsp;Xue Ao,&nbsp;Lijuan Qiu","doi":"10.1016/j.plaphy.2026.111806","DOIUrl":"10.1016/j.plaphy.2026.111806","url":null,"abstract":"<div><div>In crops, exposure to salt stress triggers pronounced water shortage, disruption of nutrient balance, and metabolic dysfunction, which collectively curtail biomass accumulation and cause substantial yield losses. Thus, identifying the genetic basis and underlying mechanisms that confer salt tolerance is therefore critical for crop improvement. However, functional validation of loci associated with salinity resilience remains limited to a small subset of genes in soybean. In this study, we comparatively evaluated plant height, root length, and fresh weight under salt stress using soybean germplasms from China, the United States, and Uruguay, and analyzed the differential salt tolerance among varieties from these regions. Subsequent genome-wide association study (GWAS) of the relative values for these three growth parameters revealed two statistically significant single-nucleotide polymorphisms (SNPs). Scanning a 200-kb linkage disequilibrium region flanking these SNPs yielded 67 candidate genes. Through integration of GWAS and transcriptomic analyses, two salt-tolerance-associated genes, <em>RRL7</em> and <em>RPH2</em>, were identified. The phenylpropanoid biosynthesis pathway, to which <em>RRL7</em> belongs, was co-enriched in both transcriptomic and metabolomic datasets. Further transcriptomic and metabolomic analysis revealed that <em>RRL7</em> is associated with increased accumulation of sinapaldehyde and 1-O-sinapoyl-β-D-glucose under salt stress. Taken together, this work advances current understanding of the genomic factors underlying soybean salt responses and identifies promising candidate genes for further functional characterization and breeding applications in soybean.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"239 ","pages":"Article 111806"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149114284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catabolism of 4-aminobutyrate (GABA) and proteinogenic amino acids sustains the tricarboxylic acid cycle in Arabidopsis thaliana (L.) Heynh. plants under salinity stress","authors":"Edward J. Flaherty,&nbsp;Gordon J. Hoover,&nbsp;Barry J. Shelp","doi":"10.1016/j.plaphy.2026.111724","DOIUrl":"10.1016/j.plaphy.2026.111724","url":null,"abstract":"<div><div>Glutamate decarboxylases1,2,4 (GAD1,2,4) are calcium/calmodulin-activated enzymes that produce 4-aminobutyrate (GABA) and eventually lead to succinate (GABA shunt), which can theoretically be incorporated into the tricarboxylic acid cycle (TCAC) during abiotic stress. We tested the hypothesis that the GABA shunt is involved in the adaptive response of the TCAC to salinity stress. Quantitative analyses of amino and organic acids, as well as <em>GAD1,2,4</em> expression, in shoots and roots of soil-grown, late-vegetative-stage <em>Arabidopsis</em> wild-type plants and <em>GAD1,2,4</em> single, double and triple loss-of function mutants provided a general understanding of the metabolic response to salinity. Salinity induced similar metabolic changes in shoots and roots, though the magnitude of the response was greater in shoots. Several mechanisms could be responsible for the non-cyclic nature of the TCAC with salinity, but inhibition of 2-oxoglutarate dehydrogenase probably resulted in the diversion of 2-oxoglutarate from the TCAC to the GABA shunt, though activation of this pathway was insufficient to sustain the succinate level observed in the absence of salinity, especially in roots. Both transcriptional and post-transcriptional regulation of GAD could have contributed to activation of the GABA shunt. Inhibition of GAD-mediated GABA production by genetic means was associated with changes in levels of free proteinogenic amino acids, as well as elevated succinate, regardless of salinity stress. Overall, GABA and proteinogenic amino acids serve an important role in ensuring flexible generation of respiratory substrate for TCAC operation and energy production.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"239 ","pages":"Article 111724"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin and auxin act synergistically to restore photosynthetic efficiency and redox homeostasis in copper-stressed Brassica juncea","authors":"Anayat Rasool Mir,&nbsp;Yamshi Arif,&nbsp;Min-Hui Bi,&nbsp;Yu-Hang Zhang,&nbsp;Di Yang,&nbsp;Lin Cai,&nbsp;Shamsul Hayat,&nbsp;Xiang-Wen Fang","doi":"10.1016/j.plaphy.2026.111849","DOIUrl":"10.1016/j.plaphy.2026.111849","url":null,"abstract":"<div><div>Copper (Cu) is an essential micronutrient involved in mitochondrial respiration, photosynthesis, and oxidative stress regulation, however, at supra-optimal concentrations it displays toxicity and inhibit plant growth and productivity. Melatonin (MT), an indoleamine with potent antioxidant activity, while as, indole-3-acetic acid (IAA), a key growth and photosynthesis promoter, both hold strong potential to mitigate abiotic stress. Considering their potential, we hypothesized that whether the antioxidant action of MT and the growth-promoting action of IAA would act synergistically or antagonistically. Results demonstrated that Cu stress enhanced the accumulation of reactive oxygen species (ROS), hydrogen peroxide (H₂O₂), superoxide (O₂⁻) malondialdehyde (MDA), and electrolyte leakage (EL) levels, thereby damaging chloroplast organization and structure, decreasing photosynthetic efficiency, CO₂ assimilation, and ultimately hinder plant growth. However, the follow-up application of IAA and/or MT proved effective in alleviating Cu-stress in plants by improving all the physio-biochemical traits via maintaining the redox homeostasis and reducing oxidative stress biomarkers. Moreover, both these indolamines proved effective to repairs the anatomical damage induced by Cu. The prominent results were obtained by combined application of both IAA and MT. The findings highlight the interplay between PGR's, antioxidant defence mechanisms and nutrient homeostasis, providing insights to develop new strategies for improving crop resilience and addressing environmental concerns associated with heavy metal accumulation in agricultural ecosystems.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"239 ","pages":"Article 111849"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149195456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conservation of the BZR-IDA/IDL regulatory module in organ abscission between litchi and Arabidopsis","authors":"Hanhan Xie,&nbsp;Zishang Kong,&nbsp;Yu Zhao,&nbsp;Dawei Qian,&nbsp;Jianguo Li,&nbsp;Minglei Zhao","doi":"10.1016/j.plaphy.2026.111844","DOIUrl":"10.1016/j.plaphy.2026.111844","url":null,"abstract":"<div><div>The IDA/IDL (INFLORESCENCE DEFICIENT IN ABSCISSION or like) peptides are conserved core components of the plant organ abscission machinery. However, the transcriptional networks governing IDA/IDL expression and their upstream regulators remain poorly understood. We previously identified LcIDL1 as a potential key positive regulator of litchi (<em>Litchi chinensis</em> Sonn.) fruitlet abscission. In the present study, yeast one-hybrid screening revealed that the <em>LcIDL1</em> promoter is directly bound by the transcription factor BRASSINAZOLE RESISTANT2 (LcBZR2), an essential constituent of the brassinosteroid (BR) signaling pathway functioning as a negative regulator of fruitlet abscission. Furthermore, we further demonstrated that LcBZR2 exerts trancriptional repression on <em>LcIDL1</em> by specifically binding to its promoter. Consistently, <em>proLcIDL1:GUS</em> signals in the abscission zone (AZ) were suppressed in a <em>35S:LcBZR2</em> background. To test the functional conservation of this regulatory module, we examined <em>Arabidopsis</em> and found that the BR-insensitive mutant <em>bri1</em> exhibits significantly accelerated floral organ abscission, elevated <em>AtIDA</em> expression in the floral AZ, and altered AZ cellular morphology. Moreover, AtBZR1 was also shown to suppress <em>AtIDA</em> transcription by specifically binding to its promoter. Collectively, these findings reveal functionally conserved BZR-IDA/IDL regulatory module in both litchi and <em>Arabidopsis</em>, which is critical for mediating organ abscission.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"239 ","pages":"Article 111844"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}