Plant Physiology and Biochemistry最新文献

{"title":"Molecular mechanism of interaction between SHORT VEGETATIVE PHASE and APETALA1 in Arabidopsis thaliana.","authors":"Qi Meng, Ya-Nan Gao, Hao Cheng, Ye Liu, Ling-Na Yuan, Man-Ru Song, Ya-Ru Li, Zi-Xin Zhao, Xiao-Fang Hou, Xiao-Min Tan, Shu-Yuan Zhang, Xuan Huang, Ye-Ye Ma, Zi-Qin Xu","doi":"10.1016/j.plaphy.2025.109512","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109512","url":null,"abstract":"<p><p>Point mutations were introduced into specific leucine (L) amino acids within the K domain of SHORT VEGETATIVE PHASE (SVP), and their effects on the SVP-AP1 interaction were assessed. Yeast two-hybrid experiments and β-galactosidase activity assays demonstrated that SVP maintained its capacity to interact with APETALA1 (AP1) despite point mutations at the 108th, 116th, 119th, and 127th leucine residues, where leucine was substituted with alanine (A). However, the mutation of the leucine residue at position 124 to alanine abolished the interaction between SVP and AP1 regardless of whether the mutation was singular or combined with others. Pull-down experiments confirmed that the leucine residue at position 124 is particularly critical for the SVP-AP1 interaction. Arabidopsis plants overexpressing 35S::AtSVP-L124A exhibited a delayed flowering phenotype compared to wild-type Col-0 Arabidopsis plants, but showed early-flowering phenotype compared to SVP overexpressing plants. SVP binds to the promoters of AP1, APETALA3 (AP3), PISTILLATA (PI), and SEPALLATA3 (SEP3), as well as to the intron of AGAMOUS (AG). Through the formation of heterodimers with AP1, SVP regulates the expression of B-class and C-class floral homeotic genes, thereby modulating floral organ development. The leucine residue at position 124 of SVP is essential for its interaction with AP1, and 35S::AtSVP-L124A transgenic plants exhibited an extended period of vegetative growth.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109512"},"PeriodicalIF":6.1,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional characterization of the C2H2 ZFP transcription factor CmSUP7 in regulating melon plant growth and fruit development.","authors":"Xinyu Dai, Zhiwei Wang, Yanfang Bao, Chenchen Jia, Fangfang Bai, Agula Hasi, Gen Che","doi":"10.1016/j.plaphy.2025.109513","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109513","url":null,"abstract":"<p><p>The SUPERMAN (SUP) proteins, which belong to the single C2H2 zinc finger proteins (ZFP) subclass, participate in various aspects of gene regulation in plant morphogenesis and stress response, but their role in melon (Cucumis melo) is still largely unknown. We identified a total of 28 CmSUP genes in the melon genome, all containing QALGGH conserved domain. Collinearity analysis showed that melon had several homologous gene pairs with Arabidopsis and tomato, indicating the gene duplication events during the evolution. Expression analyses in RT-qPCR and transcriptomic data showed that CmSUPs can be divided into vegetative organ-expressed genes and reproductive organ-expressed genes. Through genetic transformation of melons, we found that overexpression of the CmSUP7 gene causes dwarfism, reduced internode length, as well as decreased leaf and fruit size. These findings indicate that the CmSUP7 gene significantly affects the melon plant growth and fruit development. Through yeast two-hybrid and BiFC assays, we found that CmSUP7 and CmMYB14 transcription factors directly interact in the nucleus. This study comprehensively analyzed the melon CmSUP family genes and revealed the function of the CmSUP7 gene in regulating melon development, which laid the foundation for further improvement in melon breeding.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109513"},"PeriodicalIF":6.1,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZmSIDP1, a DUF1644 gene from the halophyte Zoysia matrella, positively regulates salt tolerance in rice.","authors":"Xiaohui Li, Yu Chen, Haoran Wang, Jingya Xu, Ling Zhang, Jianxiu Liu, Jianjian Li","doi":"10.1016/j.plaphy.2025.109505","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109505","url":null,"abstract":"<p><p>As a detrimental abiotic stressor, salinity affects plant growth and yield. Domain of unknown function 1644 (DUF1644) is a large plant-specific DUF protein family that is predicted to be involved in abiotic stress responses in plants. However, the biological functions of DUF1644 genes in plants remain largely unexplored, especially in halophytes. Here, we investigated the function of the DUF1644 gene, ZmSIDP1, from the halophyte Zoysia matrella. ZmSIDP1 could enhance the salt tolerance of yeast. Furthermore, the heterologous transformation of the ZmSIDP1 gene in rice demonstrated that transgenic rice plants exhibited better growth under NaCl treatment. The Na ⁺ content was lower in ZmSIDP1 transgenic rice than in wild-type rice under salt stress. ZmSIDP1 transgenic rice showed stronger resistance to oxidative stress induced by salt stress. Further investigation indicated that ZmSIDP1 could interact with an HD-Zip transcription factor, ZmROC1. These results suggest that the ZmSIDP1 gene from the halophyte Z. matrella can positively regulate salt resistance in rice, laying a foundation for the application of salt tolerance genes from halophytes to enhance salt tolerance in rice.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109505"},"PeriodicalIF":6.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular advances in research and applications of male sterility systems in tomato.","authors":"Srija Priyadarsini, Saurabh Singh, Alok Nandi","doi":"10.1016/j.plaphy.2025.109503","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109503","url":null,"abstract":"<p><p>Tomato, belonging to the nightshade family, is globally considered as a model system for classical and molecular genetics, genomics, and reproductive developmental studies. In the current scenario of climate change, hybrid development is among the crucial elements in the genetic improvement of crop plants. The phenomenon of male sterility is a viable approach for ensuring hybrid seed purity and reducing the cost of hybrid seed production. This review aims to shed light on the use of neoteric genomics and genome editing tools in understanding the genetics and molecular regulation of male sterility in tomato. Plant male gametophyte development is highly susceptible to environmental stress. Abnormalities at any stage of male reproductive development, such as premature or delayed tapetal cell degradation triggered by oxidative stress and programmed cell death (PCD) leads to male sterility in tomato. In tomato, more than 55 sporogenous, structural, and functional male sterile mutants, which are mainly under the control of recessive nuclear genes, have been reported. Recently, the role of open reading frames (ORFs) in governing cytoplasmic male sterility in tomato has also been documented. This review highlights the genetic and genomic progress in the investigation of underlying molecular pathways and practical application of potential male sterile mutants in tomato breeding. The applications and future prospects of genome engineering with CRISPR/Cas9 and mitoTALEN in the generation of novel male sterile systems to expedite tomato breeding is discussed.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109503"},"PeriodicalIF":6.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DkGASA4 plays a role in the postharvest softening of persimmon fruit regulated by gibberellin.","authors":"Ceng-Xi Xiong, Yi-Hang Zhang, Yi-Min Wang, Shen-Yue Xie, Hao-Tian Wu, Hao-Miao Ding, Wei Chen, Li-Yu Shi, Zhen-Feng Yang, Wei Wu","doi":"10.1016/j.plaphy.2025.109509","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109509","url":null,"abstract":"<p><p>Gibberellin (GA) is one of the crucial plant hormones involved in fruit ripening regulation. GASA genes, which respond to GA and encode cysteine-rich peptides, are prevalent in plants. While the GASA gene family has been identified in various plants, its role in persimmon fruit ripening remains unclear. In this study, the GASA genes of persimmon were identified and analyzed. We found that gibberellin treatment suppresses the expression of the DkGASA4. Additionally, through dual-luciferase and yeast two-hybrid assays, we discovered that DkGASA4 interacts with DkNAC9 to synergistically activate the transcription of the cell wall degradation gene DkEGase1. This study highlights the significant regulatory function of DkGASA4 in persimmon fruit ripening and softening.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109509"},"PeriodicalIF":6.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium oxalate crystals in cacao trees and their interactions with cadmium.","authors":"Fabien Letort, Eduardo Chavez, Hester Blommaert, Sylvain Campillo, Sabine Sentenac, Delphine Tisserand, Rachel Martin, Simona Denti, Géraldine Sarret","doi":"10.1016/j.plaphy.2025.109499","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109499","url":null,"abstract":"<p><p>Cadmium (Cd) concentrations in cacao beans from Latin America often exceed limits for trading. A better understanding of the mechanisms of Cd accumulation in Theobroma cacao L. trees is necessary to advance mitigation strategies. Recent studies on a high Cd accumulating cultivar of cacao revealed that calcium oxalate (CaOx) crystals were involved in Cd accumulation in the branches. The purpose of this study was to quantify soluble and crystalline oxalate in cacao compartments, to characterize their morphology and distribution in the tissues, and to evaluate the relationship between CaOx and Cd total concentrations in each plant compartment. Two representative cultivars from Latin America (CCN-51 and Nacional) were studied. CCN-51 trees grew on soils with low and high total Cd contents (0.120 ± 0.002 and 2.59 ± 0.48 mg kg⁻¹, respectively), and Nacional trees grew on soils with low Cd content (0.188 ± 0.005 mg kg⁻¹). Oxalate was present in all organs of the two cultivars. In mature leaves, oxalate content exceeded the limit of 5% per dry weight used to define extreme oxalate accumulators. The crystalline form predominated in branches and mature leaves (82-92%), whereas the soluble form predominated in nibs (67-82%). Calcium oxalate crystal size varied from <1 μm (generally agglomerated as crystal sand) to a few tens of μm (faceted crystals). Log CaOx and Cd concentrations were positively correlated in branches (R² = 0.77, p = 0.002) and roots (R² = 0.71, p = 0.005), whereas in nibs, the oxalate content was almost constant among conditions. The possible roles of CaOx crystals in the cacao plant, including Ca regulation, protection against herbivory, tissue stiffening and Cd detoxification are discussed.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109499"},"PeriodicalIF":6.1,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Taxus to paclitaxel: Opportunities and challenges for urban agriculture to promote human health.","authors":"Xiulan Xie, Yaohua Zhai, Hao Cheng, Wen-Hua Wei, Maozhi Ren","doi":"10.1016/j.plaphy.2025.109502","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109502","url":null,"abstract":"<p><p>Conifers of the genus Taxus are environmentally friendly plants with significant medicinal and ecological value, contributing to the enhancement of urban living environments. Paclitaxel, a compound found in Taxus, has garnered particular research interest owing to its potent anti-cancer effects. However, traditional methods of extracting paclitaxel from Taxus are not only inefficient, but also destructive and unsustainable, posing the major risk of Taxus extinction. To address this, sustainable production using modern biotechnology is crucial for the mass production of paclitaxel. Therefore, this review revisits the potential of Taxus and sustainable paclitaxel production in the context of urban agriculture. It provides a comprehensive review of widespread research efforts targeting efficient and cost-effective paclitaxel biosynthesis. We also discuss a set of innovative strategies for paclitaxel biosynthesis and suggest approaches for its industrial production based on Taxus cell-based approaches.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109502"},"PeriodicalIF":6.1,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of iron oxide nanoparticles on cadmium toxicity mitigation in Brassica napus.","authors":"Di He, Zohaib Kaleem, Sharafat Ali, Hafsah Shahbaz, Kangni Zhang, Juanjuan Li, Mohamed Salah Sheteiwy, Zaid Ulhassan, Weijun Zhou","doi":"10.1016/j.plaphy.2025.109500","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109500","url":null,"abstract":"<p><p>Cadmium (Cd) contamination greatly hinders plant productivity. Nanotechnology offers a promising solution for Cd phytotoxicity. The novelty of this study lies in the limited research on the effects of nanoiron (Fe₃O₄NPs) in regulating Cd toxicity in oilseed crops. This study examined how Fe₃O₄NPs regulated the Cd-exposure in B. napus. Foliar spray of 10 mg L^-1 Fe₃O₄NPs was applied to 50 μM Cd-stressed B. napus seedlings via leaf exposure in hydroponic system. Under Cd stress, Fe₃O₄NPs decreased the Cd-accumulation (25-37%) due to adsorption followed by more root Cd-immobilization, and increased the plant height (23-31%) and biomass (17-24%). These findings were directly correlated with better photosynthetic activity (chlorophylls, gas exchanges and photosynthetic efficiency), leaf stomata opening and nutrients accumulation (20-29%). Subcellular localization revealed that Fe₃O₄NPs enhanced the binding capacity of cell wall for Cd to hinder its entry into cell organalles and facilitated vacoular sequestration. Additionally, Fe₃O₄NPs decreased the oxidative stress (21-33%) and peroxidation of lipids (24-31%) by regulating the genes-associated to superoxide dismutase, peroxidase, catalase, ascorbate peroxidase, glutathione reductase, reduced glutathione, phytochelation, chlorophyll synthesis and Cd-transporters. Fe₃O₄NPs protected plant roots from Cd-induced cell structural damages and cell death. Among studied parameters, ZD 635 exhibited greater tolerance to Cd stress when compared to ZD 622 cultivar. Findings revealed that Fe₃O₄NPs effectively mitigate Cd toxicity by improving the photosynthesis, antioxidant defense mechanisms, cellular protection, nutrients accumulation and limiting Cd accumulation. This research offers a benchmark for the practical applicability of Fe₃O₄NPs to enhance the quality of canola production in Cd-contaminated soils.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109500"},"PeriodicalIF":6.1,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome assembly and multiomic analyses reveal insights into flower and bark colors of Lagerstroemia excelsa.","authors":"Zhongquan Qiao, Yi Chen, Xiaoming Wang, Yongxin Li, Sisi Liu, Fuyuan Deng, Dezhi Liao, Neng Cai, Huijie Zeng, Jianjun Chen","doi":"10.1016/j.plaphy.2025.109482","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109482","url":null,"abstract":"<p><p>Lagerstroemia excelsa is a unique plant species from China, holds a significant aesthetic and economic value, and plays a crucial role in landscape architecture and horticulture. Thus far, there is little genetic and genomic information available about this species, which limits its use in development of new cultivars. In this study, a high-quality genome map of L. excelsa was obtained via whole-genome sequencing. Results showed that its genome size is about 330.4 Mb and a scaffold mapping rate is approximately 97.20%, resulting in 24 pseudochromosomes. L. excelsa might have undergone a recent whole-genome triplication event and diverged from the pomegranate about 32.3 million years ago (MYA). Subsequently, the divergence time between L. indica and L. excelsa was around 5.9 MYA. The transcriptomic and metabolomic analyses of L. excelsa and L. indica indicated that the chalcone synthase pathway may play a key role in regulating flower color differentiation between the two species. Additionally, a transcription factor LeMYB103 may be involved in regulating anthocyanin synthesis by interacting with LeMYB66, resulting in the accumulation of anthocyanins in the stem bark. This study is the first step toward genomic analysis of L. excelsa, which may provide a foundation for further molecular investigation of this species and offer valuable insights into the molecular mechanisms underlying the flower and stem bark colors in L. excelsa, two important ornamental traits in Lagerstroemia breeding.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109482"},"PeriodicalIF":6.1,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of silver nanoparticles (NPs) and fungal elicitors on the curcuminoid production in Curcuma longa L.","authors":"Aishwarya Praveen, Kiran S Mawale, Nagarajan S, Giridhar Parvatam, Sachin R Chaudhari","doi":"10.1016/j.plaphy.2025.109501","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109501","url":null,"abstract":"<p><p>This study investigates the effects of silver nanoparticles (Ag NPs), biogenic silver nanoparticles derived from Rhizopus spp. (R.Ag NPs), and Rhizopus (R) elicitors on the yield and bioactive compounds of turmeric (Curcuma longa) using foliar spray and rhizome dipping techniques. Elicitors applied at concentrations of 10, 50, and 100 ppm over 210 days significantly enhanced turmeric yield, phenolic, flavonoid, antioxidant, and curcuminoid content compared to control plants. Among the treatments, Rhizopus culture at 50 ppm applied via foliar spray resulted in a 3.5% increase in curcuminoid content, while rhizome dipping at the same concentration led to a 3.75% increase. These findings suggest that foliar spraying and rhizome dipping with Rhizopus elicitors can effectively enhance turmeric quality, offering potential for improving crop production and curcuminoid yield.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109501"},"PeriodicalIF":6.1,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}