Plant Physiology and Biochemistry最新文献_第5页

Multi-omics analysis unveils early molecular responses to aluminum toxicity in barley root tip 多组学分析揭示大麦根尖对铝毒性的早期分子反应

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-18 DOI: 10.1016/j.plaphy.2024.109209

{"title":"Multi-omics analysis unveils early molecular responses to aluminum toxicity in barley root tip","authors":"","doi":"10.1016/j.plaphy.2024.109209","DOIUrl":"10.1016/j.plaphy.2024.109209","url":null,"abstract":"<div><div>Barley (<em>Hordeum vulgare</em> L.) is widely cultivated across diverse soil types, including acidic soils where aluminum (Al) toxicity is the major limiting factor. The relative Al sensitivity of barley highlights the need for a deeper understanding of early molecular responses in root tip (the primary target of Al toxicity) to develop Al-tolerant cultivars. Integrative <em>N</em><sup><em>6</em></sup>-methyladenosine (m6A) modification, transcriptomic, and metabolomic analyses revealed that elevated auxin and jasmonic acid (JA) levels modulated Al-induced root growth inhibition by repressing genes involved in cell elongation and proliferation. Additionally, these pathways promoted pectin demethylation via up-regulation of genes encoding pectin methylesterases (PMEs). The up-regulation of citrate efflux transporter genes including <em>Al-activated citrate transporter 1</em> (<em>HvAACT1</em>), and ATP-binding cassette (ABC) transporters like <em>HvABCB25</em>, facilitated Al exclusion and vacuolar sequestration. Enhanced activity within the phenylpropanoid pathway supported antioxidant defenses and internal chelation through the production of specific flavonoids and altered cell wall composition via lignin unit modulation. Notably, several Al-responsive genes, including <em>HvABCB25</em> and transcription factors (TFs), exhibited m6A modification changes, with two <em>microtubule associated protein 65</em> (<em>MAP65</em>) members displaying opposing regulatory patterns at both transcriptional and m6A levels, underscoring the crucial role of m6A modification in gene expression regulation. This comprehensive study provides valuable insights into the epitranscriptomic regulation of gene expression and metabolite accumulation in barley root tip under Al stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling the physiological and molecular mechanisms of leaf color change in Acer griseum through multi-omics analysis 通过多组学分析揭示灰叶槭叶色变化的生理和分子机制

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-16 DOI: 10.1016/j.plaphy.2024.109198

{"title":"Unravelling the physiological and molecular mechanisms of leaf color change in Acer griseum through multi-omics analysis","authors":"","doi":"10.1016/j.plaphy.2024.109198","DOIUrl":"10.1016/j.plaphy.2024.109198","url":null,"abstract":"<div><div>Paperbark maple (<em>Acer griseum</em>), an endemic and endangered wild plant in China, has red-colored autumn leaves of high ornamental and garden application value. Leaf color change serves as a crucial indicator for evaluating garden tree aesthetics; however, research on <em>A. griseum's</em> leaf color change remains limited. This study aims to elucidate the physiological and molecular mechanisms underlying leaf color change in maple leaves through physiological, transcriptional, and metabolic assays. Data analysis encompasses gene expression levels and metabolite changes in three distinct states of maple leaves: green, half-red, and red. The progessive decrease of chlorophyll and carotenoids and the continuous accumulation of anthocyanidins caused a sharp change in leaf coloration, which was most drastic in the green to half-red period. Subsequently, targeted metabolomics analysis was performed, and a total of 71 anthocyanidins were detected, and the content of eight types of anthocyanidins increased significantly in the half-red and red periods, compared with that in the green period; of which the multiplicative difference was the largest for cyanidin-3,5-O diglucoside, delivering the largest multiplicative difference. Thus, it was plausible that cyanidin-3,5-O-diglucoside-dominated compoundswere likely to be the main metabolites associated with leaf reddening. Correlation analysis revealed that 12 key transcription factors (TFs) were significantly correlated with the anthocyanin-related metabolites and structural genes, which play important regulatory roles during the biosynthesis of anthocyanosides in <em>A. griseum</em>. These findings offered useful insights into the molecular basis of leaf color variation in <em>A. griseum</em>; providing valuable information to guide targeted genetic breeding and varietal improvement strategies.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic responses of germination characteristics and antioxidant systems to alfalfa (Medicago sativa) seed aging based on transcriptome 基于转录组的苜蓿（Medicago sativa）种子老化对萌芽特性和抗氧化系统的动态响应。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-16 DOI: 10.1016/j.plaphy.2024.109205

{"title":"Dynamic responses of germination characteristics and antioxidant systems to alfalfa (Medicago sativa) seed aging based on transcriptome","authors":"","doi":"10.1016/j.plaphy.2024.109205","DOIUrl":"10.1016/j.plaphy.2024.109205","url":null,"abstract":"<div><div>Seed aging poses a significant challenge to agronomic production and germplasm conservation. Reactive oxygen species (ROS) are highly involved in the aging process. However, dynamic response of germination characteristics and antioxidant system to seed aging are not yet very clear. This study explored the potential physiological mechanisms responsible for the reduced and rapid loss of seed vigor in alfalfa, and identified key genes regulating seed vigor. The germination percentage exhibited a decreased trend with the prolongation of aging duration. From 16 to 32 days of aging, the antioxidant enzyme activities of SOD, POD, CAT, DHAR and MDHAR declined significantly, which lead to the disruption of ROS balance and a significant increase in ROS levels, exacerbating seed aging. Based on transcriptome, 29 differentially expressed genes (DEGs) including <em>SOD1</em>, <em>APX-2</em> and <em>GST-7</em> within the ROS scavenging system showed a significantly down-regulated expression trend at aging of 16 and 24 days, indicating the abnormal function of antioxidant metabolism. Furthermore, some related genes including <em>ATPF1B</em>, <em>ATPeF0C-3</em>, <em>NDUFS1</em>, <em>NDUFS3</em> and <em>ND2</em> in the mitochondrial ETC exhibited a downturn following seed aging, which would result in the losing of seed vigor. This study has uncovered a significant array of potential target genes within the seed antioxidant system and mitochondrial ETC. These discoveries offer a wider lens for delving into the molecular regulatory mechanisms of seed aging. Further research is crucial to comprehensively elucidate the precise pathways through which these pivotal genes regulate seed vigor.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing carotenoid accumulation in Dunaliella bardawil by combined treatments with fulvic acid and optimized culture conditions 通过富勒酸和优化培养条件的综合处理，提高杜纳藻类胡萝卜素的积累。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-16 DOI: 10.1016/j.plaphy.2024.109206

{"title":"Enhancing carotenoid accumulation in Dunaliella bardawil by combined treatments with fulvic acid and optimized culture conditions","authors":"","doi":"10.1016/j.plaphy.2024.109206","DOIUrl":"10.1016/j.plaphy.2024.109206","url":null,"abstract":"<div><div>Natural carotenoids from microalgae have received more attention as an alternative source. In this study, fulvic acid (FA), a plant growth regulator, was used to enhance carotenoid accumulation in microalgae <em>Dunaliella bardawil</em> rich in lutein. However, the addition of FA promoted pigment synthesis but also exhibited an inhibitory effect on biomass. Therefore, the optimization of culture conditions was performed to further enhance carotenoid accumulation, including high light stress (10,000 lx) and the two-stage cultivation comprising 1-aminocyclopropane-1-carboxylic acid (ACC) and FA. Under both culture conditions, the growth inhibition caused by FA was alleviated, leading to a further increase in the contents of chlorophylls and carotenoids. HPLC analysis revealed that the production of lutein, α-carotene and β-carotene increased by 0.44-, 0.37- and 0.54-fold under the treatment of 400 mg/L FA with high light intensity and 0.91-, 1.15–0.29-fold under the two-stage cultivation comprising 11 mM ACC and 500 mg/L FA. Furthermore, algal cells under FA treatment and the two-stage cultivation stained with Bodipy505/515 emitted stronger fluorescence under a laser confocal microscope, suggesting that lipid accumulation was increased. Additionally, the transcription levels of carotenogenic genes were also found to be up-regulated by qRT-PCR. These results indicated an enhancement in both the storage capacity and synthesis of carotenoids in <em>D. bardawil</em>. This study revealed the potential application of plant growth regulators in promoting carotenoid accumulation in <em>D. bardawil</em> which could be further improved by optimizing the culture conditions, providing a reference for efficient carotenoid production in microalgae.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochar solutions: Slow and fast pyrolysis effects on chromium stress in rapeseed roots 生物炭溶液：慢速和快速热解对油菜根部铬胁迫的影响

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-16 DOI: 10.1016/j.plaphy.2024.109197

{"title":"Biochar solutions: Slow and fast pyrolysis effects on chromium stress in rapeseed roots","authors":"","doi":"10.1016/j.plaphy.2024.109197","DOIUrl":"10.1016/j.plaphy.2024.109197","url":null,"abstract":"<div><div>Chromium (Cr) contamination in agricultural soils, largely due to industrial activities, poses a significant threat to plant growth and productivity. This study examines the effects of Cr stress at concentrations of 100 and 200 mg of K<sub>2</sub>Cr<sub>2</sub>O<sub>7</sub> per kg soil on rapeseed (<em>Brassica napus</em>) roots and evaluates the mitigating potential of biochar. Biochar, produced through both slow and fast pyrolysis and applied at 30 g per kg soil, was investigated for its ability to neutralize Cr toxicity. Our findings indicate that Cr stress significantly decreased the growth and physiological functions of rapeseed roots. However, biochar application improved soil pH, cation exchange capacity, and the uptake of essential nutrients such as nitrogen, phosphorus, potassium, calcium, and magnesium. Additionally, biochar enhanced the production of osmotic regulators like glycine betaine and soluble proteins, as well as indole acetic acid, promoting better root growth and water uptake under Cr stress. Notably, biochar reduced Cr availability and absorption in rapeseed roots, leading to lower levels of stress-related hormones such as abscisic acid, salicylic acid, and jasmonic acid. Among the biochars tested, slow pyrolysis biochar was more effective than fast pyrolysis biochar in mitigating Cr toxicity. These results highlight the potential of slow pyrolysis biochar as a sustainable strategy to alleviate Cr pollution and enhance plant resilience in contaminated soils.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic mild cadmium exposure increases the vulnerability of tomato plants to dehydration 长期轻度接触镉会使番茄植株更容易脱水。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-16 DOI: 10.1016/j.plaphy.2024.109200

{"title":"Chronic mild cadmium exposure increases the vulnerability of tomato plants to dehydration","authors":"","doi":"10.1016/j.plaphy.2024.109200","DOIUrl":"10.1016/j.plaphy.2024.109200","url":null,"abstract":"<div><div>Heavy metal contamination increases plant susceptibility to both biotic and abiotic stresses. However, the comprehensive impact of heavy metal pollution on plant hydraulics, which is crucial for plant productivity, and the interaction between heavy metal stress and environmental factors on plant health are not yet fully understood. In this study, we investigated the effects of cadmium exposure on plant-water relations and hydraulics of <em>Solanum lycopersicum</em> L., cultivar Piccadilly. Particular attention was given to leaf hydraulic conductance (K<sub>L</sub>) in response to cadmium pollution and dehydration.</div><div>Cadmium exposure exhibited negligible impacts on tomato productivity but resulted in significant differences in pressure-volume derived traits. Leaves and roots of Cd-treated plants showed reduced wall stiffness compared to control samples. However, Cd-treated leaves had a less negative turgor loss point (Ψ<sub>tlp</sub>), whereas Cd-treated roots exhibited more negative Ψ<sub>tlp</sub> values due to lower osmotic potential at full turgor compared to control samples.</div><div>Leaves and root cells of Cd-treated plants showed higher values of saturated water content compared to control plants, along with a distinct mineral profile between the two experimental groups. Despite similar leaf water potential thresholds for 50% and 80% loss of K<sub>L</sub> in control and cadmium-treated leaves, plants grown in cadmium-polluted soil showed higher leaf cell damages even under well watered conditions. This, in turn, affected the plant ability to recover from drought upon rehydration by compromising cell rehydration ability.</div><div>Overall, the present findings suggest that under conditions of low water availability, cadmium pollution increases the risk of leaf hydraulic failure.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SlMKK4 is responsible for pollen development in tomato SlMKK4 负责番茄的花粉发育

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-15 DOI: 10.1016/j.plaphy.2024.109201

{"title":"SlMKK4 is responsible for pollen development in tomato","authors":"","doi":"10.1016/j.plaphy.2024.109201","DOIUrl":"10.1016/j.plaphy.2024.109201","url":null,"abstract":"<div><div>The development of viable pollen is a determinant of male fertility and plays an essential role in the reproductive process of angiosperms. Mitogen-activated protein kinase (MAPK) cascades modulate diverse aspects of plant growth, but their involvement in post-meiotic pollen development is unclear. In this study, SlMKK4 was identified as a crucial regulator in overseeing pollen development in tomatoes (<em>Solanum lycopersicum</em>). Utilizing CRISPR-associated protein 9 to disrupt SlMKK4 resulted in an obvious decrease in pollen viability. The results of cell biology and transcriptomic analyses demonstrated that SlMKK4 specifically regulates auxin and sugar metabolism as well as signal transduction during post-meiotic pollen development. This is supported by the finding that protein–protein interaction assays and <em>in vitro</em> phosphorylation assays indicate that SlMKK4 serves as the upstream MAPKK for SlMPK20, which exhibits a distinct function in regulating the uninucleate (UN) to binucleate (BN) transition during microgametogenesis in tomatoes. Moreover, pollen from transgenic plants experienced significant arrest predominantly at the BN stage, accompanied by subcellular abnormalities manifesting during the late UN microspore phase. Furthermore, transcriptomic analyses indicated that SlMKK4 knockout remarkably downregulated the expression of numerous genes regulating auxin and sugar metabolism as well as signal transduction in anthers. Therefore, our findings suggest that SlMKK4 may serve as one of the upstream SlMAPKKs of SlMPK20 and also play a pivotal role in modulating post-meiotic pollen development in tomato plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating plasma activated water as a sustainable treatment for improving growth and nutrient uptake in maize and pea plant 研究等离子体活化水作为一种可持续的处理方法，用于改善玉米和豌豆的生长和养分吸收。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-15 DOI: 10.1016/j.plaphy.2024.109203

{"title":"Investigating plasma activated water as a sustainable treatment for improving growth and nutrient uptake in maize and pea plant","authors":"","doi":"10.1016/j.plaphy.2024.109203","DOIUrl":"10.1016/j.plaphy.2024.109203","url":null,"abstract":"<div><div>In this study, an atmospheric pressure air plasma jet (APAPJ) was employed to generate plasma-activated water (PAW), which was applied to treat maize (monocot) and pea (dicot) seeds for evaluating its influence. This research explored APAPJ diagnostics by varying the air feed rate as 1, 2, and 3 liter per minute (Lpm) through current-voltage characterization, optical emission spectroscopy, electron temperature and density, nitrogen metastable state density, and rotational and vibrational temperature of the plasma. Additionally, various reactive oxygen and nitrogen species (RONS) formed and physicochemical properties of PAW were analyzed by varying plasma treatment time from 0 to 8 min. Furthermore, the water uptake of maize (<em>Zea mays</em>) and pea (<em>Pisum sativum</em>) seeds were examined by the measurement of the contact angle. Results indicated that APAPJ has the capacity of fostering germination, growth, chlorophyll, phosphorus, nitrite, nitrate, ammonium ion and leaf area in plants significantly with an optimized 6 min treated PAW for maize and 2 min treated PAW for peas. Among various categories, seeds soaked in PAW and irrigated with PAW exhibited the most outstanding result in germination and plant growth. Non-thermal plasma showed promising green methods for enhancing plant growth and boosting nutrient content.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cadmium-induced protein AS8: A protein to improve Cd accumulation and transport via Cd uptake in poplar 镉诱导蛋白 AS8：通过镉吸收改善杨树镉积累和运输的蛋白质

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-15 DOI: 10.1016/j.plaphy.2024.109199

{"title":"Cadmium-induced protein AS8: A protein to improve Cd accumulation and transport via Cd uptake in poplar","authors":"","doi":"10.1016/j.plaphy.2024.109199","DOIUrl":"10.1016/j.plaphy.2024.109199","url":null,"abstract":"<div><div>The pollution of soil with heavy metals (HMs) has become an environmental problem of global concern. Phytoremediation, whereby plants extract HMs from soil, can efficiently and substantially reduce HM pollution in soil in an environmentally friendly manner. Cadmium-induced protein AS8 (CIPAS8) is present in many plants and its expression is induced by HMs. In this study, <em>PeCIPAS8</em> and <em>SlCIPAS8</em> were transformed into 84K poplar to study their effects on tolerance to, and translocation of, cadmium (Cd) in woody plants. Localization analyses showed that two CIPAS8 proteins were localized at the plasma membrane when transiently expressed in tobacco leaf epidermal cells. Compared with wild-type 84K poplar seedlings, transgenic poplar lines overexpressing <em>PeCIPAS8</em> or <em>SlCIPAS8</em> showed increased Cd contents and decreased Cd tolerance. Transgenic poplar lines overexpressing <em>PeCIPAS8</em> or <em>SlCIPAS8</em> accumulated more Cd in the roots, stems, and leaves, but the plant height did not differ significantly, compared with wild-type 84K poplar under Cd stress during the vegetative stage. CIPAS8 increased the Cd influx rate of transgenic poplar roots compared with that of the wild type, and affected the transcription levels of other metal transporters. These findings show that CIPAS8 increases Cd flux into plant tissues and demonstrate moderate Cd sensitivity of the plant. Therefore, CIPAS8 is an influx transporter with the potential to increase the uptake of toxic HMs by woody plants growing in HM-contaminated soils.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role and mechanism of TgCWIN2-mediated changes of photo-assimilates in modulating early development of Torreya grandis seeds TgCWIN2 介导的光同化物变化在调节香榧种子早期发育中的作用和机制

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-14 DOI: 10.1016/j.plaphy.2024.109188

{"title":"The role and mechanism of TgCWIN2-mediated changes of photo-assimilates in modulating early development of Torreya grandis seeds","authors":"","doi":"10.1016/j.plaphy.2024.109188","DOIUrl":"10.1016/j.plaphy.2024.109188","url":null,"abstract":"<div><div>Early seed development is vital for plant reproduction, but the processes behind this in gymnosperms like <em>Torreya grandis</em>, which has a low rate of normal early-developed seeds, are not well understood. To fill this knowledge gap, we embarked on a comprehensive investigation encompassing the morphology and phenology of seed development in <em>T. grandis</em>. Using the <sup>13</sup>C labelling analysis, coupled with leaf removal and seed thinning treatments, we observed a substantial increase in the content of photo-assimilate, an almost 10% increase in sucrose content under seed thinning treatments, thereby leading to an increase in the proportion of normal early-developed seeds, reaching 15%. Concurrently, through the integration of multi-omics analyses and transient overexpression validation, we identified cell wall invertase coding gene, <em>TgCWIN2</em>, which plays a pivotal role in sucrose cleavage during the early development of <em>T. grandis</em> seeds. Further gene co-expression, dual-luciferase assay, and yeast one-hybrid assay revealed that TgWRKY31 was a candidate regulator of <em>TgCWIN2</em>, positively influencing its expression by direct binding to the <em>TgCWIN2</em> promoter. Notably, <em>TgWRKY31</em> transient overexpression substantially enhances the expression of <em>TgCWIN2</em>, thereby contributing to a higher proportion of normal early-developed seeds. Our findings not only provide a comprehensive understanding of the underlying mechanisms governing the early development of <em>T. grandis</em> seeds, but are also essential for establishing strategies to enhance early seed development and improve yield.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0