Plant Physiology and Biochemistry最新文献

{"title":"Effects of exogenous melatonin on growth and photosynthesis of maize (Zea mays L.) seedlings under low nitrogen stress","authors":"Xiangyue Lv,&nbsp;Qing Zhang,&nbsp;Jihang He,&nbsp;Yi Yang,&nbsp;Zhenqing Xia,&nbsp;Yuxiang Gong,&nbsp;Jianchao Liu,&nbsp;Haidong Lu","doi":"10.1016/j.plaphy.2025.109810","DOIUrl":"10.1016/j.plaphy.2025.109810","url":null,"abstract":"<div><div>Nitrogen plays an important role in plant growth and development. Melatonin (Mel) is involved in many physiological and biochemical reactions in plants, which can enhance the resistance of plants to abiotic stress. However, the mechanism of exogenous Mel in maize seedlings under low nitrogen stress is still unknown. This experiment adopts the method of sand culture, Zhengdan 958 was used as the experimental variety, and three nitrogen levels (5mmolL⁻¹, 2mmolL⁻¹, 0.1mmolL⁻¹) and two Mel treatment conditions (no exogenous Mel and 1μmolL-1 exogenous Mel) were set up to explore the effects of exogenous Mel on the growth and photosynthesis of maize seedlings under low nitrogen stress. The growth of maize seedlings under low nitrogen stress was significantly inhibited. Exogenous melatonin (Mel) increased the shoot dry weight by 11.74 % and 17.57 % under mild and severe low nitrogen stress, respectively. It promoted root growth, enhanced nitrogen accumulation in the shoots, and improved the net photosynthetic rate of maize seedlings, with increases of 14.85 % and 24.77 % under mild and severe low nitrogen stress, respectively.Exogenous Mel improved the activity of the reaction centers in photosystem I (PSI) and photosystem II (PSII), increased electron transport rates, thereby enhancing light energy conversion efficiency and allocating more light energy to photochemical reactions. Additionally, exogenous Mel effectively alleviated the photo-oxidative damage to the photosynthetic systems caused by low nitrogen stress. This study demonstrated that exogenous Mel could significantly mitigate the inhibitory effects of low nitrogen stress on maize seedling growth.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109810"},"PeriodicalIF":6.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143682548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking ectopic lignification in flax stems following scarification","authors":"Anne-Sophie Blervacq ,&nbsp;Dmitry Galinousky ,&nbsp;Clémence Simon ,&nbsp;Myriam Moreau ,&nbsp;Anne Duputié ,&nbsp;Fabien Baldacci-Cresp ,&nbsp;Cedric Lion ,&nbsp;Christophe Biot ,&nbsp;Simon Hawkins ,&nbsp;Godfrey Neutelings","doi":"10.1016/j.plaphy.2025.109806","DOIUrl":"10.1016/j.plaphy.2025.109806","url":null,"abstract":"<div><div>When flax (<em>Linum usitatissimum</em> L.) stems are scarified, major changes occur in the organization of cell walls within the tissues that border the wound. We sought to characterize the plant's response using a variety of approaches, with a particular focus on lignin deposition within the peripheral fiber cell walls of the stem. Raman spectroscopy and imaging first showed that changes occurred in the polysaccharide matrix of the parenchyma and fiber cell walls. These changes were accompanied by rapid deposition of lignin which initially diffuses centripetally and then, once the vascular cambium was reached, propagates in a periclinal manner until 150 μm from the edges of the wounded zone. Lignin biosynthesis appears to be the result of a <em>de novo</em> activity, as demonstrated by the concomitant accumulation of transcripts corresponding to lignin biosynthesis genes. In addition, using bioorthogonal chemistry approaches, we showed that wounding had enhanced the capacity of fiber cell walls to incorporate modified lignin precursors, in parallel with an increase in transcripts corresponding to peroxidases in the cortical tissues. This incorporation potential was identical for the 3 different types of reporters tested. Our findings demonstrated that mechanical stress can trigger lignification, in a polarized manner within the bast fibers, providing insights into the plasticity of cell wall composition and the potential for modulating fiber properties in flax.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109806"},"PeriodicalIF":6.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative approaches unravelling tea drought alleviation mechanisms primed by carbonyl volatiles and signal peptide","authors":"Abdelkader Bassiony ,&nbsp;Shujuan Sun ,&nbsp;Kangni Yan ,&nbsp;Qunhua Peng ,&nbsp;Mengxue Zhou ,&nbsp;Jianyu Fu ,&nbsp;Susanne Baldermann ,&nbsp;Jiang Shi ,&nbsp;Haipeng Lv ,&nbsp;Zhi Lin","doi":"10.1016/j.plaphy.2025.109802","DOIUrl":"10.1016/j.plaphy.2025.109802","url":null,"abstract":"<div><div>Drought stress (DS) significantly hampers the growth and productivity of tea plants, necessitating effective strategies to enhance their resilience. This study comprehensively investigated the mechanisms of carbonyl volatiles-methyl jasmonate (MeJA) and cis-3-hexenyl acetate (<em>cis</em>-3-HAC) and signal peptide CLAVATA3/EMBRYO-SURROUNDING REGION-RELATED 25 (CLE25) promotion DS resistance using integrative metabolomics and proteomics strategy. Total pigment content decreased, while soluble sugar and proteins increased significantly under DS and further increased after foliar inducement of CLE25, MeJA, and <em>cis</em>-3-HAC. Gallated catechins and amino acids exhibited apparent decreased under DS, especially EGCG (24.6–13.4 mg g⁻¹) and theanine (10.66–3.78 mg g⁻¹), but significantly mitigated by CLE25 inducement. Antioxidant enzymes activity, such as catalase (CAT), jumped from 23.1 to 48.2 and further boosted to 118.8 Ug⁻¹min⁻¹ FW with CLE25. Proteomic analysis revealed massive increased in stress tolerance proteins, particularly dehydrins and heat shock proteins, rising by &gt;50.0 % with CLE25 inducement and the expression levels of peroxidase (POX), superoxide dismutase (SOD), α-galactosidase (α-GAL), carboxypeptidases (CPs), and transaldolase (TAL) exhibited higher after inducement. Furthermore, stress signaling-related proteins were in-depth explored, especially thioredoxin proteins; sucrose non-fermenting 1-related protein kinase 2 (SnRK2) was novelly verified in activating abscisic acid (ABA) responding. Differences among drought resistance mechanisms after carbonyl volatile and CLE25 treatments were comprehensively studied. The integration of metabolite and protein levels provided a comprehensive illustration of tea DS tolerance mechanisms and offered promising promotion strategies through foliar application of MeJA, <em>cis</em>-3-HAC, and CLE25.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109802"},"PeriodicalIF":6.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of ADF gene family in Chinese cabbage (Brassica rapa L. ssp. pekinensis) and functional characterization of BrADF11 under heat stress","authors":"Wei Wang ,&nbsp;Gaoyang Qu ,&nbsp;Yifei Sun ,&nbsp;Jingyi Chen ,&nbsp;Hui Feng ,&nbsp;Yue Gao","doi":"10.1016/j.plaphy.2025.109796","DOIUrl":"10.1016/j.plaphy.2025.109796","url":null,"abstract":"<div><div>Actin depolymerizing factors (ADFs), which function as essential actin-binding proteins (ABPs), are fundamental to plant growth, development, and stress responses by depolymerizing or severing actin filaments. However, research on the <em>ADF</em> gene family in Chinese cabbage remains relatively scarce. In Chinese cabbage, we identified 18 <em>BrADF</em> genes unevenly distributed across eight chromosomes. Phylogenetic analysis revealed that <em>BrADF</em> genes can be classified into four subfamilies. Cis-regulatory elements related to stress response and hormone signaling response were distributed in the promoter sequences of these genes. Expression analysis showed variability in the expression patterns of <em>BrADF</em> genes across different tissues, with most genes responding to heat stress; notably, <em>BrADF11</em> showed significant upregulation under heat stress conditions. Furthermore, transient silencing and transient overexpression experiments proved that <em>BrADF11</em> plays a negative regulatory role in the heat tolerance of Chinese cabbage. In conclusion, this study not only systematically analyzed the characteristics of <em>ADF</em> gene family in Chinese cabbage, but also laid a solid foundation for studying the function of Chinese Cabbage <em>ADF</em> genes under abiotic stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109796"},"PeriodicalIF":6.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143682547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dihydroflavonol 4-reductase from Iris lactea has distinct substrate specificity and promoting the synthesis of delphinidin-based anthocyanins","authors":"Hong Zhang ,&nbsp;Juan Hu ,&nbsp;Gege Tian ,&nbsp;Yumeng Zhao ,&nbsp;Jing Wang ,&nbsp;Sirui Lang ,&nbsp;Ren Chen","doi":"10.1016/j.plaphy.2025.109805","DOIUrl":"10.1016/j.plaphy.2025.109805","url":null,"abstract":"<div><div>Anthocyanin, a water-soluble flavonoid pigment, serves as a key secondary metabolite and plays a major role in the formation of color in plant flowers, fruits and vegetables. Dihydroflavonol 4-reductase (DFR) is a key enzyme in the anthocyanin biosynthesis pathway, catalyzing the reduction of dihydroflavonols into leucoanthocyanidins. In this study, we presented the identification of a putative <em>IlDFR</em> gene from <em>Iris lactea</em> Pall var. chinensis. The amino acid sequences of IlDFR shares an evolutionary lineage among its same genus, and IlDFR showed high activity when dihydromyricetin (DHM) was used as a substrate, while less or no activity using dihydrokaempferol (DHK) or dihydroquercetin (DHQ) as a substrate in the enzymatic assay. This may be the reason way the <em>I. lactea</em> exhibits blue-purple color because it mainly biosynthesizes and accumulates delphinidins in its petals. The <em>IlDFR</em> expressed in a white flower variety of <em>Petunia</em>×<em>hybrida</em> converted noticeable different phenotypes that exhibited light to dark purple in their flowers. In a transgenic plant with dark purple flower and the highest expression level of <em>IlDFR</em> showed that the contents of delphinidin-based anthocyanins, including delphinidin and its methylated derivative petunidin, as well as their glycosides (glucoside, rutinoside, galactoside and sophoroside) were significantly in higher levels than those of no-transgenic negative control. These results further strengthened the evidence that IlDFR prefers DHM substrate. Our research will provide new gene resources and a basis for color modification of flowers, fruits and vegetables using molecular biology and genetic engineering techniques.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109805"},"PeriodicalIF":6.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Genome-wide identification and characterization of alfalfa-specific genes in drought stress tolerance\" [Plant Physiol. Biochem. 220 (2025) 109474].","authors":"Yitong Ma, Qingyan Zhai, Zhipeng Liu, Wenxian Liu","doi":"10.1016/j.plaphy.2025.109776","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109776","url":null,"abstract":"","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":" ","pages":"109776"},"PeriodicalIF":6.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redox regulation of autophagy in Arabidopsis: The different ROS effects","authors":"Germán Robert ,&nbsp;Alejandro Enet ,&nbsp;Laura Saavedra ,&nbsp;Ramiro Lascano","doi":"10.1016/j.plaphy.2025.109800","DOIUrl":"10.1016/j.plaphy.2025.109800","url":null,"abstract":"<div><div>Autophagy plays a key role in the responses to different stress condition in plants. Reactive oxygen species (ROS) are common modulators of stress responses, having both toxic and signaling functions. In this context, the relationship between ROS and autophagy regulation remains unclear, and in some aspects, contradictory. In this study, we employed pharmacological and genetic approaches to investigate the effects of different ROS on the cytoplastic redox state and autophagic flux in <em>Arabidopsis thaliana</em>. Our results demonstrated that oxidative treatments with H₂O₂ and MV, which drastically increased the oxidized state of the cytoplasm, reduced the autophagic flux. Conversely, singlet oxygen, which did not have significant effects on the cytoplasmic redox state, increased the autophagic flux. Additionally, our findings indicated that after H₂O₂ and high light treatments and during the recovery period, the cytoplasm returned to its reduced state, while autophagy was markedly induced. In summary, our study unveils the differential effects of ROS on the autophagic flux, establishing a correlation with the redox state of the cytoplasm. Moreover, it emphasizes the dynamic nature of autophagy in response to oxidative stress and the subsequent recovery period.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109800"},"PeriodicalIF":6.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen-rich water enhances vegetable growth and fruit quality by regulating ascorbate biosynthesis","authors":"Bei Lin ,&nbsp;Jinyi Lin ,&nbsp;Zhiyu Song ,&nbsp;Miao Zhang ,&nbsp;Ying Chen ,&nbsp;Yujia Ma ,&nbsp;Weimin Xu ,&nbsp;Shilong Sun ,&nbsp;Zhen Luan ,&nbsp;Lihong Gao ,&nbsp;Wenna Zhang","doi":"10.1016/j.plaphy.2025.109790","DOIUrl":"10.1016/j.plaphy.2025.109790","url":null,"abstract":"<div><div>Under aerobic conditions, the growth and fruit quality of vegetable crops are significantly influenced by reactive oxygen species (ROS) metabolism. Hydrogen-rich water (HRW) has emerged as a promising tool for enhancing resistance to abiotic stresses and delaying postharvest ripening and senescence. However, the physiological response and adaptation mechanisms of vegetable crops to HRW remain rarely understood. This study explores the effects of low concentrations of HRW on the growth and physiological processes of lettuce, tomato, and cucumber. The results indicate that HRW enhances seedling vigor, boosts photosynthetic efficiency, and promotes biomass accumulation. Additionally, HRW-irrigated cucumber fruit showed a 15–20 % increase in vitamin C (ascorbic acid) content, a 10–15 % rise in soluble sucrose levels, and an increase in fruit weight and diameter by 25–35 % and 8–12 %, respectively. Transcriptomic analyses revealed variations in genes associated with carbon fixation in photosynthesis, glyoxylate and dicarboxylate metabolism, hormonal regulation, and phenylalanine metabolism. These findings illuminate the mechanisms behind improved antioxidant production and L-ascorbate biosynthesis. Notably, this marks the documented case of HRW irrigation enhancing natural antioxidants in fruits. Given the unique properties of hydrogen and the potential of HRW technology in horticultural industry, the findings of this study provide valuable insights into hydrogen's role in biological processes and its impact on vegetable crops production and fruit quality.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109790"},"PeriodicalIF":6.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143682542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of CiHY5 in salt tolerance of Chrysanthemum indicum and conserved role of HY5 under stress in chrysanthemum","authors":"Bin Xia ,&nbsp;Ziwei Li ,&nbsp;Xiaowei Liu ,&nbsp;Yujia Yang ,&nbsp;Shengyan Chen ,&nbsp;Bin Chen ,&nbsp;Ning Xu ,&nbsp;Jinxiu Han ,&nbsp;Yunwei Zhou ,&nbsp;Miao He","doi":"10.1016/j.plaphy.2025.109797","DOIUrl":"10.1016/j.plaphy.2025.109797","url":null,"abstract":"<div><div>Among various abiotic stresses, secondary soil salinization poses a significant threat to plant productivity and survival. Cultivated chrysanthemums (<em>Chrysanthemum morifolium</em>), widely grown as ornamental crops, are highly susceptible to salt stress, and their complex polyploid genome complicates the identification of stress resistance genes. In contrast, <em>C</em>. <em>indicum</em>, a native diploid species with robust stress tolerance, serves as a valuable genetic resource for uncovering stress-responsive genes and improving the resilience of ornamental chrysanthemum cultivars. In this study, we cloned, overexpressed (OE-CiHY5), and silenced (RNAi-CiHY5) the <em>CiHY5</em> gene in <em>C. indicum</em>. OE-CiHY5 plants exhibited larger leaves, sturdier stalks, and higher chlorophyll content compared to wild-type plants, while RNAi-CiHY5 plants displayed weaker growth. Under salt stress, OE-CiHY5 plants demonstrated significantly improved growth, enhanced osmotic adjustment, and effective ROS scavenging. In contrast, RNAi-CiHY5 plants were more sensitive to salinity, showing higher electrolyte leakage and impaired osmotic regulation. Transcriptomic analyses revealed that <em>CiHY5</em> regulates key hormonal pathways such as zeatin (one of cytokinins), abscisic acid and jasmonic acid, as well as metabolic pathways, including photosynthesis, carbohydrate metabolism, which collectively contribute to the enhanced stress resilience of OE-CiHY5 plants. Promoter-binding assays further confirmed that <em>CiHY5</em> directly interacts with the <em>CiABF3</em> promoter, highlighting its critical role in ABA signaling. Evolutionary analyses showed that HY5 is conserved across plant lineages, from early algae to advanced angiosperms, with consistent responsiveness to salt and other abiotic stresses in multiple <em>Chrysanthemum</em> species. These findings establish <em>CiHY5</em> as a key regulator of salt tolerance in <em>C. indicum</em>, orchestrating a complex network of hormonal and metabolic pathways to mitigate salinity-induced damage. Given the conserved nature of HY5 and its responsiveness to various stresses, <em>HY5</em> gene provides valuable insights into the molecular mechanisms underlying stress adaptation and represents a promising genetic target for enhancing salt stress resilience in chrysanthemums.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109797"},"PeriodicalIF":6.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two homoeoallelic gene expression of TaCHLIs ensures normal chlorophyll biosynthesis in Hexaploid wheat","authors":"Jian Yang ,&nbsp;Yankun Zhao ,&nbsp;Yanmin Zou ,&nbsp;Jinfu Ban ,&nbsp;Zhankun Li ,&nbsp;Yu'e Zhang ,&nbsp;Junfeng Yang ,&nbsp;Yan Wang ,&nbsp;Caihua Li ,&nbsp;Xiaoyi Fu ,&nbsp;Xinmei Gao ,&nbsp;Weiguo Hu ,&nbsp;Xicheng Wang ,&nbsp;Yanjie Zhou ,&nbsp;Xin Ding ,&nbsp;Mingqi He ,&nbsp;Wensheng Zhang ,&nbsp;Tingjie Cao ,&nbsp;Zhenxian Gao","doi":"10.1016/j.plaphy.2025.109795","DOIUrl":"10.1016/j.plaphy.2025.109795","url":null,"abstract":"<div><div>Being polyploid has a fitness advantage but is physically complex. During polyploid plant evolution, some duplicate genes retain their ancestral function, which affected the plant phenotype in allelic dosage or functional redundancy. However, how duplicated genes whose products needed to form functional complexes coped with deleterious mutations remained unclear. Here, we report a <em>yellow green leaf-2</em> (<em>ygl2</em>) mutant with yellow-green leaves derived from a cross of Shaan 3025 (S3025) and Shi 4185 (S4185) that was controlled by a combination of <em>Tachli-7A</em> null and <em>Tachli-7B</em> truncation, whereas <em>Tachli-7A</em> null or <em>Tachli-7B</em> truncation individually resulted in normal leaf colour. Our results indicated genetic complementarity between <em>TaCHLI-7A</em> and <em>TaCHLI-7B</em> is responsible for normal chloroplast development. Furthermore, <em>TaCHLI-7D</em> was conserved in <em>ygl2</em>, S3025 and S4185 at both sequence and expression levels. Furthermore, two-thirds of the total mRNA abundance in S4185 with <em>Tachli-7A</em> null was sufficient for chlorophyll synthesis, indicating that redundant mRNA dosage was the reason for genetic complementarity. Particularly, <em>Tachli-7A</em> null can be retained in several modern cultivars with no disadvantage under field conditions, probably because the redundant mRNA dosage is expected to buffer the gene imbalance caused by the imperfect relationship between different copies of <em>TaCHLIs</em> and their molecular interactors. Furthermore, the loss of <em>TaCHLI-7A</em> seems to preserve the minimum dosage and maximise simplification. Our findings provide evidence of homoeologs loss and functional mechanism during polyploid evolution.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109795"},"PeriodicalIF":6.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143682544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}