Plant Physiology and Biochemistry最新文献

{"title":"Chitosan nanoparticles and germanium synergistically enhance photosynthetic efficiency, sugar metabolism, and anthocyanin biosynthesis via metabolic pathway modulation in guar","authors":"Seham M. Hamed ,&nbsp;Uğur Tan ,&nbsp;Marwa Yousry A. Mohamed ,&nbsp;Ashraf Khalifa ,&nbsp;Hamada AbdElgawad","doi":"10.1016/j.plaphy.2025.110566","DOIUrl":"10.1016/j.plaphy.2025.110566","url":null,"abstract":"<div><div>Boosting crop yield and quality is critical for feeding the world's population. The synergistic interaction of germanium (Ge) and chitosan nanoparticles (CSNPs) offers a novel approach to enhance guar (<em>Cyamopsis tetragonoloba</em>) yield and metabolism. This synergy led to substantial increases in guar biomass and yield, ranging from 33 % to 41 %, which correlated with improved photosynthesis. Improved photosynthesis induced sugar metabolism in leaves and seeds that directed to biosynthesis of primary metabolites including essential amino acids, organic acids (17.5–35.5 %), and lipids shifted toward unsaturated fatty acids. At the seed level, Ge and CSNPs significantly elevated crude protein, lipid, fiber, and sugar contents (r &gt; 0.65–0.99). Anthocyanin levels in leaves increased significantly, reflecting efficient metabolic channeling and avoidance of bottlenecks in precursor accumulation (like phenylalanine) and enzymatic activity (e.g., UDP-glucose: flavonoid 3-O-glucosyltransferase saw a &gt;4-fold increase). Furthermore, Ge + CSNPs boosted seed phosphorus by 27.9 % and antioxidant capacity by 57.4 %, which improved overall nutritional quality. Principal component analysis (71.65 % variance) confirmed that Ge + CSNPs coordinate metabolic changes, linking sugar availability and anthocyanin metabolism to increased guar yield. This study shows that Ge + CSNPs optimize metabolic transitions, offering a sustainable way to improve guar productivity, and nutritive values.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110566"},"PeriodicalIF":5.7,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA responses to alkaline stress and the key role of the miR156–MsSPL2/6B module in alfalfa","authors":"Tongtong Yao ,&nbsp;Siyue Qi ,&nbsp;Hongjiao Zhang ,&nbsp;Hongrui Zhang ,&nbsp;Jiang Su ,&nbsp;Zhongyong Cen ,&nbsp;Zheyuan Wang ,&nbsp;Bo Qin ,&nbsp;Huihui Zhang","doi":"10.1016/j.plaphy.2025.110567","DOIUrl":"10.1016/j.plaphy.2025.110567","url":null,"abstract":"<div><div>The global expansion of saline-alkali soils has made alkaline stress a major abiotic factor limiting plant growth and crop yield. Alfalfa (<em>Medicago sativa</em> L.), as an important high-quality forage grass, also has its growth and development significantly been constrained by alkaline stress. To investigate the molecular mechanisms underlying alfalfa's response to alkaline stress, this study focused on two previously identified alfalfa cultivars with distinct tolerance levels: the tolerant “ZD (Zhaodong)” and the sensitive “ZM (Zhongmu No.1).” Using miRNA-Seq and RNA-Seq, we systematically analyzed the expression changes of miRNAs and mRNAs in both cultivars under alkaline stress, aiming to identify key regulatory miRNAs and their target gene modules. The analysis identified 112 miRNAs that were significantly differentially expressed miRNAs (DEMs) following NaHCO₃ treatment. Integrative miRNA-mRNA analysis revealed 258 high-confidence miRNA-target gene interaction pairs. Functional enrichment of DEMs and differentially expressed genes (DEGs) highlighted several biological processes and signaling pathways closely associated with stress response, including transcription factor regulation, phenylpropanoid biosynthesis, flavonoid metabolism, plant hormone signaling, and calcium (Ca²⁺) signaling. Notably, many genes related to hormone and Ca²⁺ signaling were reportedly regulated by differentially expressed miRNAs, exhibiting significant expression differences between the two cultivars. This underscores their critical role in balancing growth and stress response. Additionally, numerous differentially expressed miRNAs indirectly regulate alfalfa's alkaline tolerance by targeting transcription factors, with the miR156-SPL module identified as a potentially key contributor to alkaline stress adaptation. Transient co-expression assays demonstrated that <em>Ms-miR156</em> target to <em>MsSPL2</em> and <em>MsSPL6B</em>. Overexpression of <em>MsSPL2</em> and <em>MsSPL6B</em> in Arabidopsis enhanced tolerance to alkaline stress, alleviating its adverse effects on root growth, photoinhibition, and oxidative damage. In summary, this study systematically elucidates the miRNA-mediated regulatory network underlying alfalfa's response to alkaline stress, providing a theoretical foundation and potential molecular targets for understanding and improving salt-alkali tolerance in alfalfa.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110567"},"PeriodicalIF":5.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transcription factor bZIP44 enhances lead tolerance by activating the expression of PDR12 in Arabidopsis","authors":"Xi Wu ,&nbsp;Qian Ma ,&nbsp;Zhen Zhang ,&nbsp;Mengfan Wu ,&nbsp;Xinyun Liao ,&nbsp;Guangna Chen ,&nbsp;Hui Song ,&nbsp;Shuqing Cao","doi":"10.1016/j.plaphy.2025.110559","DOIUrl":"10.1016/j.plaphy.2025.110559","url":null,"abstract":"<div><div>Lead (Pb) contamination poses a severe threat to plant growth and ecosystem health. Although plants have evolved various detoxification mechanisms, the transcriptional regulation underlying Pb tolerance remains poorly understood. Here, we demonstrate that the transcription factor bZIP44 plays a critical role in mediating Pb stress responses in Arabidopsis. Expression of <em>bZIP44</em> was induced by Pb stress. The <em>bzip44</em> mutants showed increased sensitivity to Pb stress and enhanced accumulation of Pb, whereas the <em>bZIP44</em> overexpression lines were tolerant to Pb stress and reduced Pb accumulation. Further studies revealed that bZIP44 directly binds to the promoter of <em>PDR12</em>, an ABC transporter gene essential for Pb efflux, and activates its expression under Pb stress. Genetic analysis showed that bZIP44 is located upstream of PDR12 and positively regulates the Pb stress response in Arabidopsis. Our findings identify bZIP44 as a key upstream regulator of PDR12 and establish the bZIP44–PDR12 module as a central pathway in Pb detoxification, providing new insights into the molecular mechanisms of heavy metal tolerance in plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110559"},"PeriodicalIF":5.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of TCP gene family and functional analysis of PcTCP8 gene related to trichome development in Pogostemon cablin","authors":"Yingying Liang ,&nbsp;Xiaoqi Peng ,&nbsp;Yuwei Zhang ,&nbsp;Siyu Xie ,&nbsp;Qixuan Huang ,&nbsp;Lu Yang ,&nbsp;Chang Su ,&nbsp;Huili Lai ,&nbsp;Wenru Wu","doi":"10.1016/j.plaphy.2025.110570","DOIUrl":"10.1016/j.plaphy.2025.110570","url":null,"abstract":"<div><div>Trichomes are key sites for synthesis and storage of plant secondary metabolites. The TCP transcription factor family is essential for trichomes cell fate determination. In <em>Pogostemon cablin</em>, trichome density significantly affects the accumulation of terpenoid metabolites, but the role of TCP gene family in this process is unclear. This study characterized TCP proteins in <em>P. cablin</em> and explored the impact of <em>PcTCP8</em> on trichome development and volatile compound biosynthesis. It identified 44 PcTCP proteins and used transcriptome data for expression profiling, providing functional insights. Over-expression of <em>PcTCP8</em> decreased patchoulol content, virus-induced gene silencing (VIGS) of <em>PcTCP8</em> resulted in increased trichome density, elevated patchoulol levels, and upregulated expression of <em>PcFFPS</em>. These findings demonstrate that <em>PcTCP8</em> acts as a negative regulator of both glandular trichome development and essential oil biosynthesis in <em>P. cablin</em>. The study provides novel insights into the genetic regulation of glandular trichomes in medicinal plants, offering valuable information for future research in this field.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110570"},"PeriodicalIF":5.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The GARP-ARR-B family transcription factor SeAPRR2 positively regulates drought tolerance in chayote (Sechium edule)","authors":"Shaobo Cheng,&nbsp;Zhili Chen,&nbsp;Wei Yan,&nbsp;Lihong Su,&nbsp;Xiaoting Zhou,&nbsp;Zhongqun He","doi":"10.1016/j.plaphy.2025.110561","DOIUrl":"10.1016/j.plaphy.2025.110561","url":null,"abstract":"<div><div>Chayote, an economically vital cucurbit crop, faces severe production and quality constraints due to increasing drought stress. Despite this threat, systematic identification and analysis of the GARP-ARR-B transcription factor family in chayote remain unreported. This study identified 11 ARR-B transcription factors in chayote, with focused investigation on the drought-responsive gene <em>SeAPRR2</em>. <em>SeAPRR2</em> exhibited rapid induction under PEG-simulated drought, and its heterologous expression significantly enhanced osmotic stress tolerance in yeast. Furthermore, <em>SeAPRR2</em> overexpression in tomato substantially improved drought resistance, manifested through reduced wilting, decreased electrolyte leakage, lower malondialdehyde content, elevated proline accumulation, attenuated reactive oxygen species (ROS), and accelerated stomatal closure. The interaction between <em>SeAPRR2</em> and the metallochaperone <em>SeHIPP3</em> was confirmed by Y2H, LCI, BIFC and molecular docking. Additionally, DAP-seq, Y1H, and LUC assays demonstrated <em>SeAPRR2</em>'s direct binding and transcriptional activation of <em>SeP5CS1</em> and <em>SeNCED5</em>. Collectively, <em>SeAPRR2</em> synchronously enhances drought tolerance through proline-mediated osmotic adjustment and ABA-triggered stomatal closure, suggesting its potential as a candidate gene for enhancing drought tolerance in chayote germplasm.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110561"},"PeriodicalIF":5.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of root anatomy, phytochemicals and gene expression in bolted and unbolted Saposhnikovia divaricata","authors":"Xiao Liu ,&nbsp;Xijia Cui ,&nbsp;Xiran Bi ,&nbsp;Yi Cui ,&nbsp;Yunhe Wang ,&nbsp;Hongmei Lin ,&nbsp;Zhuo Sun ,&nbsp;Lin Cheng ,&nbsp;Limin Yang ,&nbsp;Zhongming Han ,&nbsp;Jian Zhang","doi":"10.1016/j.plaphy.2025.110568","DOIUrl":"10.1016/j.plaphy.2025.110568","url":null,"abstract":"<div><div><em>Saposhnikovia divaricata</em> (Turcz.) Schischk., known as Fangfeng, is a highly valued traditional Chinese medicinal herb esteemed for its therapeutic properties. Premature bolting in <em>S. divaricata</em> adversely affects root yield and medicinal quality. This study aimed to compare root anatomical structures, active phytochemical contents, and gene expression differences between unbolted (UBF) and bolted (BF) <em>S. divaricata</em> plants, which can provide a theoretical foundation for elucidating potential mechanisms driving premature bolting for future research and practical applications. The result showed that UBF roots exhibited intact secondary xylem and wider secondary phloem, whereas BF roots showed fragmented secondary xylem with lignified parenchyma cells. Chromone concentrations were higher in UBF plants, particularly within the secondary phloem. Transcriptome analysis identified 33 differentially expressed genes (DEGs) associated with bolting and flowering, 22 DEGs involved in plant hormone signal transduction pathways, including ETR, JAR1, EIN3, TCH4, GID2, ABF, BKI1, BSK, BIN, BZR1/2, CYCD3, and 11 DEGs involved in circadian rhythm pathways, including GI, ZTL, FT, PHYA, COP1, SPA, FKF1, were differentially expressed between BF and UBF groups, suggesting their potential role in regulating bolting and flowering in <em>S. divaricata</em>. These findings suggest that plant hormones and circadian rhythms may influence bolting and flowering in <em>S. divaricata</em>. These findings can provide a theoretical basis for analyzing the mechanisms of bolting and flowering in this species and the Apiaceae family<em>.</em> However, premature bolting adversely affects root quality, necessitating further investigation into its regulatory mechanisms to improve cultivation practices.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110568"},"PeriodicalIF":5.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photorespiration and plant immunity: Interactions and implications under a changing climate","authors":"Jingying Chen ,&nbsp;Yafei Yan ,&nbsp;Shuangchen Chen,&nbsp;Golam Jalal Ahammed","doi":"10.1016/j.plaphy.2025.110569","DOIUrl":"10.1016/j.plaphy.2025.110569","url":null,"abstract":"<div><div>Climate change has profound impacts on plant growth, productivity, and immunity. Photosynthesis, a key biological process that sequesters atmospheric CO₂, plays a crucial role in mitigating the effects of climate change. However, photorespiration, a process that consumes O₂ instead of CO₂ and is metabolically linked with photosynthesis, is often viewed as a wasteful process that reduces photosynthetic efficiency by nearly 48 % in C₃ plants. This reduction has a significant impact on crop yield, given the direct contribution of photosynthesis to biomass accumulation. Over the years, numerous efforts have been made to rectify this perceived metabolic flaw to enhance photosynthetic efficiency. Interestingly, recent studies have unveiled a role for photorespiration in plant immunity, which can vary from positive to negative depending on the plant-pathosystem. A key challenge lies in enhancing photosynthetic efficiency by modulating photorespiration without compromising plant immunity. This review discusses the role of photorespiration in plant immunity under current and future climatic conditions. We explore how photorespiration and photorespiratory pathways influence plant defense, how alterations in photorespiration affect hormonal pathways and subsequently plant immunity, and how manipulations of photorespiration may impact plant growth and defense under elevated CO₂ conditions. We highlight the roles of plant hormones such as salicylates and jasmonates as well as reactive oxygen species, in photorespiration-related plant immunity. We conclude that unraveling the underlying mechanisms of photorespiration-involved plant responses to various pathogens provides comprehensive insights for the management, breeding, and genetic improvement of crops, thereby enhancing their resilience to future climate change.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110569"},"PeriodicalIF":5.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the cell wall nitrogen use efficiency – Can the differences between cell wall architectures contribute to the nitrogen economy of plants?","authors":"Gabriela Ellen Barreto Bossoni ,&nbsp;Matt Stata ,&nbsp;Aline Marengoni Almeida ,&nbsp;Rodrigo Polimeni Constantin ,&nbsp;Rogério Marchiosi ,&nbsp;Osvaldo Ferrarese-Filho ,&nbsp;Rowan F. Sage ,&nbsp;Wanderley Dantas dos Santos","doi":"10.1016/j.plaphy.2025.110548","DOIUrl":"10.1016/j.plaphy.2025.110548","url":null,"abstract":"<div><div>C4 plants exhibit greater nitrogen use efficiency (NUE) than C3 plants, primarily due to lower ribulose-1,5-bisphosphate carboxylase/oxygenase requirements. However, plant NUE also varies among C4 species, suggesting that other factors, such as cell wall composition, contribute to nitrogen economy. To investigate the contribution of cell wall architecture to nitrogen economy, we compared plant species with distinct cell wall types: eudicots with type I cell walls (T1CW) and grasses with type II cell walls (T2CW), under four nitrogen regimes: deficit, low, medium, and normal. Species with different photosynthetic metabolisms were compared to confirm known differences in photosynthetic nitrogen use efficiency (pNUE), while species with similar metabolism but distinct cell wall types were compared assessing the influence of cell wall on nitrogen economy. The pNUE of C4 grasses was higher than that of C4 eudicots, increasing from +54 % in normal nitrogen to +81 % in nitrogen deficit. C4 grasses presented lower structural nitrogen (−26 %) in normal nitrogen supply, which decreased to −58 % in nitrogen deficit, in comparison to C4 eudicots. An exploratory parameter <em>cwpNUE</em> (photosynthetic rate/structural nitrogen) resulted in a much higher value in C4 grasses (∼1.1 μmol CO₂ s⁻¹ mmol⁻¹ cell wall nitrogen) than in the other groups (∼0.5 μmol CO₂ s⁻¹ mmol⁻¹ cell wall nitrogen). In turn, the ester-linked ferulic acid increased from +177 % in C4 grasses in normal nitrogen to +362 % in nitrogen deficit, when compared to C4 eudicots. Data supports the hypothesis that in hot, humid tropical environments, nitrogen became a major limiting nutrient for C4 plant growth and development. The overlap between extensins and FA-GAX in crosslinking CW polymers observed in T2CW suggest that nitrogen scarcity may have exerted a selection pressure for adaptations in C4 grasses to contribute to NUE.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110548"},"PeriodicalIF":5.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PscCYP716A1-mediated brassinolide biosynthesis increases lead tolerance and enrichment in poplar","authors":"Jie Yang ,&nbsp;Keran Zhu ,&nbsp;Xiaoxi Chen ,&nbsp;Feifei Tian ,&nbsp;Chengyu Han ,&nbsp;Zhikun Liang ,&nbsp;Kaixuan Xiao ,&nbsp;Ruixue Hao ,&nbsp;Meng Liu ,&nbsp;Xiangyu Zhou ,&nbsp;Xueqin Wan ,&nbsp;Qinglin Liu ,&nbsp;Fan Zhang","doi":"10.1016/j.plaphy.2025.110564","DOIUrl":"10.1016/j.plaphy.2025.110564","url":null,"abstract":"<div><div>Urban soil lead (Pb) pollution poses a pressing threat to ecosystems and public health. Phytoremediation has emerged as an effective solution to address this issue. Poplars are widely employed in urban greening owing to their fast growth and high biomass, rendering them prime candidates for Pb remediation. However, their innate remediation capacity remains constrained, therefore, it needs to further enhance by genetic improvement. Transcriptome sequencing (RNA-seq) has identified that <em>PscCYP716A1</em>, a gene involved in brassinosteroid (BR) synthesis, is upregulated under Pb stress, indicating a potential role in Pb tolerance. This prompted a research question: Can <em>PscCYP716A1</em> improve poplar's Pb tolerance? In this study, we obtained <em>PscCYP716A1</em>-overexpression poplars (OE lines, OE-13 and OE-42) and RNA interference <em>PscCYP716A1</em>-expression poplars (RNAi lines, RI-33 and RI-23). The overexpression poplars significantly enhanced Pb tolerance, and increased Pb accumulation compared to wild-type (WT). Further analysis revealed that <em>PscCYP716A1</em> enhances Pb tolerance by improving the antioxidant system and Pb chelation. However, when exogenous brassinazole (BR biosynthesis inhibitor, BRZ) was sprayed on poplar under Pb stress, all advantages of OE lines disappeared. According to the results of analysis, the improvement of antioxidant and chelation capacities conferred by <em>PscCYP716A1</em> may be related to its participation in the BR synthesis pathway. In summary, our results revealed the physiological mechanism and detoxification processes facilitated by <em>PscCYP716A1</em>, and underscored its potential as a genetic tool for enhancing phytoremediation in Pb-contaminated urban environments. These findings provide a theoretical foundation and technical support for the application of engineered poplars in remediating soil Pb pollution.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110564"},"PeriodicalIF":5.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tonoplast sugar transporter StTST1 mediates vacuolar sugar partitioning and abiotic stress tolerance in potato","authors":"Jin Wang ,&nbsp;Md Abu Kawocha ,&nbsp;Tengfei Liu ,&nbsp;Tiantian Liu ,&nbsp;Jingjing Guo ,&nbsp;Shulan Hu ,&nbsp;Yi Liu ,&nbsp;Shengxuan Liu ,&nbsp;Lin Chen ,&nbsp;Bihua Nie ,&nbsp;Botao Song","doi":"10.1016/j.plaphy.2025.110549","DOIUrl":"10.1016/j.plaphy.2025.110549","url":null,"abstract":"<div><div>Sugar transporters play pivotal roles in plant growth, development, and stress responses. However, the function of sugar transporters in potato (Solanum tuberosum) is still obscure. In this study, the function of potato tonoplast sugar transporter 1 (StTST1) in subcellular sugar compartmentation and abiotic stress tolerance was characterized. Heterologous expression assays in <em>Saccharomyces cerevisiae</em> (strain W303) demonstrated that StTST1 mediates sucrose transporting into the vacuole. Moreover overexpression and RNA interference (RNAi) of <em>StTST1</em> in potato altered leafy sugar content including sucrose, glucose, and fructose without affecting the activity of key metabolic enzymes. Intriguingly, RNAi-mediated suppression of <em>StTST1</em> enhanced freezing tolerance and compromised drought tolerance. In contrast, overexpression of StTST1 enhanced drought tolerance but reduced freezing tolerance. Our results demonstrate that StTST1 dynamically regulates subcellular sugar partitioning and differentially modulates freezing and drought stress responses. These findings highlight the potential of targeted manipulation of sugar transporters to modulate crop resilience to multiple abiotic stresses.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110549"},"PeriodicalIF":5.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}