Plant Physiology and Biochemistry最新文献

{"title":"Transcriptome mapping of unfertilized pea flowers under high temperature: insights into gene regulatory networks genomics resources","authors":"Chanderkant Chaudhary ,&nbsp;Meenakshi Kanwar ,&nbsp;Parul Sirohi ,&nbsp;Kumar Ankit Anand ,&nbsp;Sohini Barua ,&nbsp;Jaswinder Singh ,&nbsp;Harsh Chauhan","doi":"10.1016/j.plaphy.2025.110268","DOIUrl":"10.1016/j.plaphy.2025.110268","url":null,"abstract":"<div><div>Heat stress is the most significant environmental constraint on pea production, and heat tolerance mechanisms are mediated through a variety of pathways. Pea (<em>Pisum sativum</em> L.) cultivar Arka Chaitra (AC) is considered a heat tolerant variety, whereas cultivar Matar Ageta (MA) is heat sensitive. Transcriptome profiling of two pea cultivars, AC and MA, was conducted under control and heat stress (HS) conditions to elucidate the molecular mechanisms and identify genes associated with heat tolerance. In this study, we initially reported the repertoire of morpho-physiological traits namely pod number (PNP), weight of pods (PW), seed number/pod (SNP) and weight of seed/pod (SW) during HS conditions in AC and MA. Subsequently, Pollen viability assay was performed to further examine the pollen behavior under HS. Morpho-physiological analysis revealed that cultivar AC exhibited greater number of pods and seeds, as well as higher pod and seed weights, compared to MA under HS. Furthermore, pollen viability in cultivar AC was reduced by 23 %, whereas in cultivar MA, it was reduced by 41 %, indicating that AC exhibits robust tolerance mechanisms under HS conditions. Based on these observations, we delve deeper to investigate the regulatory mechanisms by profiling the transcriptomes of cultivars AC and MA using RNA Sequencing (RNA-Seq) under HS. We selected a subset of genes, including heat shock transcription factors, and heat shock proteins involved in heat resilience, to validate the RNA-Seq expression patterns using quantitative RT-PCR, which revealed higher expression levels in AC compared to MA. Furthermore, gene ontology (GO) analysis of the identified DEGs revealed significant enrichment of GO terms associated with pollen and flower development, heat stress proteins, and plastid development in the tolerant variety, whereas, the susceptible variety was enriched with GO terms related to cell morphogenesis, cell growth, and cell wall biogenesis. These findings provide <em>in-depth</em> analysis of genes and pathways contributing to thermotolerance in pea cultivars AC and MA.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110268"},"PeriodicalIF":6.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated physiological, transcriptomic and metabolomic analysis revealed heterosis for cadmium tolerance in maize","authors":"Pingxi Wang,&nbsp;Min Li,&nbsp;Xingye Ma,&nbsp;Bin Zhao,&nbsp;Xining Jin,&nbsp;Shilin Chen,&nbsp;Xiaoxiang Zhang,&nbsp;Xiangyuan Wu,&nbsp;Huaisheng Zhang","doi":"10.1016/j.plaphy.2025.110265","DOIUrl":"10.1016/j.plaphy.2025.110265","url":null,"abstract":"<div><div>Heterosis describes superior performance of F₁ hybrids over parents in yield and stress tolerance, yet its role in Cd tolerance remains poorly characterized in maize. This study integrated physiological, transcriptomic, and metabolomic analyses for the hybrid Zhengdan958 (ZD958) and its parents under cadmium (Cd) stress. Compared to parental lines, ZD958 consistently exhibited superior morphological traits (e.g., plant height, root biomass) and significantly enhanced elevated catalase (CAT) activity under Cd stress. Mid-parent heterosis (MPH) for key traits ranged from 2.73 % to 25.90 %, confirming robust hybrid vigor under Cd exposure. Transcriptomic analysis identified 904 unique differentially expressed genes (DEGs) in ZD958 under Cd stress, with weighted gene co-expression network analysis (WGCNA) revealing two key modules. Metabolomic analysis identified 902 metabolites, and the differentially accumulated metabolites in ZD958 primarily enriched in phenylpropanoid biosynthesis, glycerophospholipid metabolism, and glycosylphosphatidylinositol-anchor biosynthesis. Analysis of non-additive expression (NAE) genes identified one gene under both conditions, which was also specifically down-regulated in ZD958 under Cd stress. Analysis of allele-specific expression (ASE) genes revealed 12 genetically over-dominant genes in ZD958. Integrated multi-omics analysis highlighted the critical roles of phenylpropanoid biosynthesis and starch and sucrose metabolism in the heterosis of ZD958 to Cd stress. Our findings provide novel insights into how phenylpropanoid biosynthesis and starch/sucrose metabolism mediate heterosis for Cd tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110265"},"PeriodicalIF":6.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling root growth dynamics and rhizosphere microbial responses to waterlogging stress in rapeseed seedlings","authors":"Aqarahim Wasim ,&nbsp;Xiaohua Bian ,&nbsp;Fangyuan Huang ,&nbsp;Ximin Zhi ,&nbsp;Yifan Cao ,&nbsp;Siyu Gun ,&nbsp;Yuexia Zhang ,&nbsp;Ni Ma","doi":"10.1016/j.plaphy.2025.110269","DOIUrl":"10.1016/j.plaphy.2025.110269","url":null,"abstract":"<div><div>Waterlogging is a major abiotic stress that significantly alters ecological biodiversity and threatens crop growth and productivity. Rhizosphere microbial communities are essential for crop mineral nutrition and can help plants withstand stress. However, little is known about how rapeseed rhizosphere microbes respond to waterlogging and their link with soil nutrient availability and root architecture, and physiology. Thus, this study compared root morphological and physiological traits, soil physicochemical properties, and changes in rhizosphere microbial community composition and diversity using 16S rDNA and internal transcribed spacer (ITS) amplicon sequencing under waterlogging and normal conditions. The waterlogging stress induced a significant decline in root activity, growth characteristics, and content of nitrogen and potassium. In contrast, the phosphorus content of roots and shoots significantly increased. Rhizosphere bacteria richness and evenness decreased under waterlogging stress, which was associated with a significant decrease in soil available nitrogen, phosphorus, and potassium. In contrast, fungal diversity increased under waterlogging, concordant with increased soil organic matter content after five days. Notably, the bacterial phyla Proteobacteria and Firmicutes, and fungal phyla Ascomycota and Basidiomycota were identified as biomarkers under waterlogging. Interestingly, several Plant Growth-Promoting Rhizobacteria, including <em>Pseudomonas</em> and <em>Rhizobium</em>, increased under waterlogging conditions, indicating their potential for enhancing rapeseed mineral nutrition, growth, and tolerance. Besides, harmful fungi such as <em>Fusarium solani</em> increased under waterlogging conditions. Our results enhance our understanding of rapeseed response to waterlogging and the contribution of rhizosphere bacteria and fungi. Moreover, the current study provides theoretical bases for improving rapeseed waterlogging tolerance through soil management.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110269"},"PeriodicalIF":6.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The sugarcane ScMYB3R1-ScPYL61-ScPP2C57 module confers drought tolerance via ABA signaling","authors":"Keyi Luo ,&nbsp;Kai Chen ,&nbsp;Lifang Zeng,&nbsp;Mingkun Chen,&nbsp;Pingping Lin,&nbsp;Xianman Dong,&nbsp;Shuo Jiang,&nbsp;Wei Yao,&nbsp;Muqing Zhang,&nbsp;Qin Hu,&nbsp;Baoqi Li,&nbsp;Shenghua Xiao","doi":"10.1016/j.plaphy.2025.110247","DOIUrl":"10.1016/j.plaphy.2025.110247","url":null,"abstract":"<div><div>Drought stress constitutes a significant challenge for plant growth, particularly impacting sugarcane yield and quality. MYB transcription factors play a pivotal role in regulating drought tolerance in plants, yet investigations into the 3R-MYB subfamily remain limited. Here, we identified 35 sugarcane 3R-MYB genes, in which <em>ScMYB3R1</em> significantly responds to PEG and ABA treatments. The overexpression of <em>ScMYB3R1</em> enhanced drought tolerance in Arabidopsis through reducing water loss and activating ABA signaling. Through global gene expression profiling, we found that numerous differentially expressed genes were enriched in ABA signaling and drought stress response pathways. Notably, <em>ScMYB3R1</em> regulated 28.8% of ABA-induced genes and 29.9% of ABA-repressed genes. Further analysis revealed that ScMYB3R1 physically interacts with the ABA receptor ScPYL61, and they collaboratively activate ABA signaling. Additionally, ScPYL61 interacts with the type 2C protein phosphatase ScPP2C57, and ScMYB3R1 promotes this interaction. These findings collectively reveal a novel molecular module, ScMYB3R1-ScPYL61-ScPP2C57, in sugarcane that contributes to drought tolerance in an ABA-dependent manner. This research not only identifies potential candidate genes for improving drought tolerance in sugarcane but also expands our understanding of the functional roles of the 3R-MYB subfamily and ABA signaling mechanisms.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110247"},"PeriodicalIF":6.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and phytohormonal profiling of the adventitious shoot regeneration of Camellia reticulata and functional characterization of the CrSAUR20 gene","authors":"Xuan Wang ,&nbsp;Jianzhao Wang ,&nbsp;Yinxin Yang ,&nbsp;Mei Dao ,&nbsp;Yan Bai ,&nbsp;Yuan Gao ,&nbsp;Longqing Chen ,&nbsp;Tian Wu","doi":"10.1016/j.plaphy.2025.110259","DOIUrl":"10.1016/j.plaphy.2025.110259","url":null,"abstract":"<div><div>In establishing <em>in vitro</em> regeneration of <em>Camellia reticulata</em>, we found that the callus from the wild <em>C. reticulata</em> plant could differentiate into the adventitious shoot (AS). In contrast, the callus from <em>C. reticulata</em> cultivar ‘Purple Gown’ has not yet differentiated into the AS, and the mechanism of AS regeneration was still unclear. The molecular mechanisms underlying AS regeneration were investigated using transcriptomic profiling and phytohormone quantification. Results showed that the levels of cytokinin, auxin, and other hormones in the callus of the wild <em>C. reticulata</em> plant were significantly changed compared with those of ‘Purple Gown’. Transcriptomic profiling identified differentially expressed genes (DEGs) involved in phytohormone signaling and hormone biosynthesis pathways. Among these, the expression of response regulators (<em>ARRs</em>), auxin response factors (<em>ARFs</em>), indole-3-pyruvate monooxygenase (<em>YUCCAs</em>), small auxin up-regulated RNAs (<em>SAURs</em>), ethylene-responsive transcription factor 1/2 (<em>ERF1/2</em>), and others significantly differed between the wild <em>C. reticulata</em> plant and ‘Purple Gown’, affecting the development of AS in the callus of the <em>C. reticulata</em>. To further validate the gene function related to hormones, <em>CrSAUR20</em> was cloned as an example and its overexpression vector was constructed. The overexpression of <em>CrSAUR20</em> promoted an increased ratio of auxin-to-cytokinin in transgenic tobacco plants and affected the differentiation of AS and root from the leaf disc of transgenic tobacco plants, suggesting a positive regulatory role of <em>CrSAUR20</em> in auxin signaling transduction. Genotype, phytohormone ratio, and phytohormone crosstalk were critical factors in enhancing AS regeneration capacity in the callus of <em>C. reticulata</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110259"},"PeriodicalIF":6.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome-metabolome convergence reveals that BsPP2C17 and BsPP2C27 of Bletilla striata (Orchidaceae) significantly regulate ABA-mediated seed germination","authors":"Shuai Liu,&nbsp;Siting Zheng,&nbsp;Kaifeng Gou,&nbsp;Ruohan Huang,&nbsp;Shiqiang Wang,&nbsp;Donghao Wang,&nbsp;Junfeng Niu,&nbsp;Zhezhi Wang","doi":"10.1016/j.plaphy.2025.110241","DOIUrl":"10.1016/j.plaphy.2025.110241","url":null,"abstract":"<div><div>Orchidaceae seeds are tiny and undeveloped, commonly facing difficulties in germination under natural conditions. Therefore, investigating seed germination of orchids is critically important. In this study, <em>Bletilla striata</em> seed germination was completed, the germination process was divided into six stages through morphological and histocytological observation, including ungerminated seeds (BS0), imbibition (BS1), formation of rhizoids (BS2), protocorm (BS3), radicle (BS4) and plumule (BS5). LC-MS analysis showed that ABA concentration increased and then decreased, while auxins and cytokinins concentration gradually decreased, and various carbohydrates contents gradually increased. Exogenous ABA treatment showed that ABA significantly inhibited the germination of <em>B. striata</em>. RNA-seq analysis found 323 genes were significantly differentially expressed during protocorms formation (p &lt; 0.01), with more than 4-fold change. Two genes in ABA signaling pathway, <em>BsPP2C17</em> and <em>BsPP2C27</em>, were upregulated by 30-fold and 60-fold, respectively. Heterologous expression in Arabidopsis showed that they could respectively revert the insensitivity to ABA of <em>atap2c4</em> and <em>atpp2c5</em> during seed germination, and the overexpression lines <em>Col-OE-BsPP2C17</em> and <em>Col-OE-BsPP2C27</em> exhibited hypersensitivity to ABA signals. These demonstrated that <em>BsPP2C17</em> and <em>BsPP2C27</em> were involved in the regulation of Arabidopsis seed germination as positive regulators of ABA signaling. These results provided a new perspective for the study of seed germination in Orchidaceae.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110241"},"PeriodicalIF":6.1,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive metabolomic and transcriptomic analyses of the anthocyanin accumulation mechanism in the leaf veins of two Broussonetia papyrifera varieties (ZJ and CL) under cold stress","authors":"Xiangxue Yu ,&nbsp;Rui Ni ,&nbsp;Mei Wang ,&nbsp;Bingxin Jia ,&nbsp;Bo Chen ,&nbsp;Qian Li ,&nbsp;Guoqing Hou ,&nbsp;Ziyu Yang ,&nbsp;Chenjing Li ,&nbsp;Fuyuan Liu ,&nbsp;Yongtao Xia ,&nbsp;Jingyi Zhao ,&nbsp;Zhijia Tian ,&nbsp;Li Zhang ,&nbsp;Xinyong Guo","doi":"10.1016/j.plaphy.2025.110248","DOIUrl":"10.1016/j.plaphy.2025.110248","url":null,"abstract":"<div><div><em>Broussonetia papyrifera</em> is an important native tree species in China. However, its relatively weak cold tolerance not only significantly compromises its survival and reproductive abilities in cold regions but also limits the realization of its economic potential. Notably, previous studies have demonstrated that anthocyanin accumulation enhances plant cold resistance. Despite this, the precise mechanisms underlying anthocyanin accumulation in <em>B. papyrifera</em> remain unclear. Therefore, this study aims to elucidate the pathways and regulatory mechanisms of anthocyanin accumulation in <em>B. papyrifera</em> under cold stress. Two varieties of <em>B. papyrifera</em> (ZJ and CL), were subjected to cold stress at 4 °C. After 21 days, a marked accumulation of anthocyanins was observed in the veins of CL, whereas severe yellowing occurred in the veins of ZJ. Quantitative analysis revealed that the anthocyanin content in CL-V-DW was significantly higher than in other groups. Integrated transcriptomic and metabolomic analyses demonstrated that differentially expressed genes were predominantly enriched in flavonoid metabolic pathways associated with anthocyanin biosynthesis. Cold stress induced upregulation of most anthocyanin synthesis-related genes in both ZJ and CL; however, the expression of glucosyltransferases <em>(BpGST)</em> genes in ZJ-V-DW was markedly reduced, leading to impaired transport of synthesized anthocyanins. Furthermore, genes involved in photosynthesis and chlorophyll/carotenoid metabolism were suppressed, accelerating senescence in ZJ. Metabolomic profiling identified 60 anthocyanin-related metabolites, with Cyanidin-3-O-rutinoside being the predominant pigment in CL-V-DW. The integrated analysis of the transcriptome and metabolome identified 12 differentially expressed genes (DEGs), 15 structural genes, and 20 transcription factors, revealing significant correlations between these genes and anthocyanin-3-O-rutinoside, particularly <em>BpABR1</em> and chalcone isomerase <em>(BpCHI)</em>. Notably, the accumulation of anthocyanins in CL partially attenuated photosynthetic activity. This study elucidates that CL sustains anthocyanin synthesis through the activation of <em>BpCHI, Ethylene-responsive transcription factor ABR1 (BpABR1)</em>, and WRKY transcription factor 40 (<em>BpWRKY40)</em>, while enhancing anthocyanin transport efficiency via <em>BpGST</em> activation. Collectively, these findings provide insights into the molecular mechanisms underlying vein color regulation in <em>B. papyrifera</em> under cold stress and offer a theoretical basis for breeding cold-tolerant varieties.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110248"},"PeriodicalIF":5.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted metabolomics analysis in tolerant and susceptible landraces of rice under sodicity stress","authors":"Manoharan Akilan ,&nbsp;Paramasiwam Jeyaprakash ,&nbsp;Murugappan Shanmuganathan ,&nbsp;Suresh Meena ,&nbsp;Venugopal Rajanbabu ,&nbsp;Adhimoolam Karthikeyan ,&nbsp;Gunasekaran Ariharasutharsan ,&nbsp;Kathiresan Pravin Kumar ,&nbsp;Palanisamy Savitha ,&nbsp;Sadayandi Geethanjali ,&nbsp;Sampathrajan Vellaikumar ,&nbsp;Chocklingam Vanniarajan","doi":"10.1016/j.plaphy.2025.110251","DOIUrl":"10.1016/j.plaphy.2025.110251","url":null,"abstract":"<div><div>Sodicity is a major abiotic stress affecting rice production by disrupting soil structure and impairing major physiological processes in plants. Landraces possess high genetic diversity and exhibit strong adaptation to diverse environmental stresses, including sodicity. In the present study, we screened 47 landraces and identified <em>Norungan</em> as highly tolerant to sodicity based on morpho-physiological traits. Further, we characterized the biochemical and metabolic responses of <em>Norungan</em> and <em>Vellai Kudaivazhai</em> (Highly susceptible landrace) under control and sodicity stress conditions. Biochemical analysis revealed that efficient photosynthesis, reduced lipid peroxidation, and elevated antioxidant enzyme activities distinguished <em>Norungan</em> from <em>Vellai Kudaivazhai</em> and provided primary evidence supporting its tolerance. Untargeted metabolomics analysis of <em>Norungan</em> and <em>Vellai Kudaivazhai</em> identified 103 and 117 differentially accumulated metabolites (DAMs), respectively. The DAMs in <em>Norungan</em> indicated more efficient energy utilization through the TCA cycle and GABA shunt compared to <em>Vellai Kudaivazhai</em>, along with effective sucrose homeostasis that minimized carbon leakage <em>via</em> dead cells. Although, <em>Norungan</em> exhibited effective sucrose homeostasis, minimizing carbon leakage through dead cells, the upregulation of major osmolytes, such as proline (Fold change – 5.32) further underscored its tolerance. Additionally, the enrichment of pathways such as starch and sucrose metabolism, and the TCA cycle, highlighting <em>Norungan</em>'s adaptive mechanisms underlying sodicity tolerance. Taken together, these findings provide valuable insights into the mechanisms of sodicity tolerance, which may inform targeted breeding strategies aimed at improving sodicity tolerance in rice.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110251"},"PeriodicalIF":6.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of tonoplast sugar transporter gene HpTST1 involved in soluble sugars accumulation during fruit development process of red pitaya (Hylocereus polyrhizus)","authors":"Qian-Ming Zheng ,&nbsp;Hong-Lin Wang ,&nbsp;Shuang Yan ,&nbsp;Pu Xie ,&nbsp;Yong Qi","doi":"10.1016/j.plaphy.2025.110228","DOIUrl":"10.1016/j.plaphy.2025.110228","url":null,"abstract":"<div><div>Soluble sugars are considered to be the most important components to determine the flavor and quality of fleshy fruits. Tonoplast sugar transports (TSTs) mediate sugar uptake into vacuoles and play pivotal roles in plant growth and development. Herein, we performed expression analysis of red pitaya <em>TST</em>s, selected a candidate isoform <em>HpTST1</em> and verified its transport activities and biological functions. During red pitaya fruit development, we observed a significant rise in glucose and fructose levels, which were the primary soluble sugars in ripe fruit. Among four <em>HpTST</em>s, <em>HpTST1</em> presented the highest expression of an isoform and showed the up-regulation expression pattern that correlated soluble sugars accumulation. Histochemical analysis using a promoter-driven GUS reporter in Arabidopsis indicated that <em>HpTST1</em> was widely expressed across most source and sink tissues. Subcellular localization studies confirmed that HpTST1 was targeted to the tonoplast in plant cells. Functional assays in baker's yeast demonstrated that HpTST1 was capable of transporting monosaccharides (glucose, fructose and 2-deoxy-D-glucose), sucrose and its analogue esculin. Transient expression of <em>HpTST1</em> in strawberry fruits significantly increased sucrose, glucose and fructose levels. Moreover, heterologous expression of <em>HpTST1</em> in tomatoes resulted in an enhanced plant growth and accelerated flowering process. Importantly, compared to wild-type, transgenic lines showed heavier fruit weight by 23.8 %, higher levels of soluble solid contents by 22.7 %, fructose and glucose contents by 35.6–44.4 %. Our research suggests that HpTST1 is a positive regulator of soluble sugars accumulation in red pitaya fruits, thus enhancing our understanding of molecular mechanisms regulating soluble sugars accumulation in fruit vacuoles.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110228"},"PeriodicalIF":6.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jasmonic acid enhances aluminum tolerance of rice by regulating detoxification genes","authors":"Chun Yan Tu ,&nbsp;Hao Yu Wang ,&nbsp;Qi Jiang ,&nbsp;Yong Qiang Gao ,&nbsp;Ren Fang Shen ,&nbsp;Xiao Fang Zhu","doi":"10.1016/j.plaphy.2025.110257","DOIUrl":"10.1016/j.plaphy.2025.110257","url":null,"abstract":"<div><div>Aluminum (Al) stress in acidified soils represents a critical limitation for rice productivity and sustainable cropping. Jasmonic acid (JA) plays a crucial role in plant responses to Al stress, yet its underlying molecular mechanisms remain poorly understood. This study aimed to elucidate the role and mechanisms of JA in mitigating Al toxicity in rice. We found that Al stress upregulates the expression of the genes associated with JA biosynthesis in rice roots, leading to elevated endogenous JA levels, implying its involvement in Al stress responses. Exogenous JA application significantly reduced Al accumulation in root tips and alleviated Al-induced root growth inhibition. Mechanistically, JA upregulated the expression of the transcription factor <em>OsWRKY22</em> (<em>WRKY GENE</em> 22), citrate transporter genes <em>OsFRDL4</em> and <em>OsFRDL2</em> (<em>FRD3-LIKE PROTEIN 2/4</em>), and the Al-binding protein gene <em>OsCDT3</em> (<em>CADMIUM TOLERANCE 3</em>), thereby preventing the entrance of the Al into the cytoplasm. Furthermore, JA increased nitric oxide (NO) levels in root tips, alleviating root growth inhibition. These findings demonstrate that JA mitigates Al toxicity through enhancing NO-mediated stress tolerance and restricting Al uptake, providing new insights into the molecular basis of JA-mediated Al resistance in rice.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110257"},"PeriodicalIF":6.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}