Plant Physiology and Biochemistry最新文献

{"title":"Combining transcriptome and metabolome analyses to reveal the response of maize roots to Pb stress","authors":"Xiaoxiang Zhang ,&nbsp;Bin Zhao ,&nbsp;Xingye Ma ,&nbsp;Xining Jin ,&nbsp;Shilin Chen ,&nbsp;Pingxi Wang ,&nbsp;Guan Zhongrong ,&nbsp;Xiangyuan Wu ,&nbsp;Huaisheng Zhang","doi":"10.1016/j.plaphy.2024.109265","DOIUrl":"10.1016/j.plaphy.2024.109265","url":null,"abstract":"<div><div>As a major food crop, maize (<em>Zea mays</em> L.) is facing a serious threat of lead (Pb) pollution. Research into its Pb tolerance is crucial for ensuring food security and human health, however, the molecular mechanism underlying the response to Pb remains incompletely understood. Here, we investigated the transcriptomic and metabolome of two maize lines (BY001, a Pb-resistant line; BY006, a Pb-sensitive line) under different concentrations of Pb stress (0, 500, 1000, 2000 and 3000 mg/L). The results showed that BY001 performed well, whereas the BY006 exhibited minimal development of lateral roots upon exposure to high concentration of Pb. The antioxidant enzyme activity of BY001 remained relatively stable, while that of BY006 declined significantly. Transcriptomic analysis revealed that under high concentration of Pb stress, BY001 produced 5057 differentially expressed genes, whereas BY006 produced 3374. Functional annotation showed that these genes were primarily involved in carbohydrate metabolism, root growth, and plant resistance to external Pb stress. Further untargeted metabolomics indicated that Pb stress triggered distinct alterations in the levels of 47 diverse metabolite types across 13 distinct classes, particularly amino acids, carbohydrates, and organic acids. A conjoint omics analysis suggested that the pathways of starch and sucrose metabolism, as well as cutin, suberin, and wax biosynthesis in BY001, play a key role in the Pb resistance. These findings elucidate the biological mechanisms employed by maize to counter the effects of Pb stress, and provide a basis for breeding of maize cultivars with low Pb accumulation or tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109265"},"PeriodicalIF":6.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Four ClEF1A genes involved in self-incompatibility in 'Xiangshui Lemon' confer early fowering and increase stress tolerance in transgenic Arabidopsis","authors":"Moying Lan ,&nbsp;Kaijiang Li ,&nbsp;Cong Luo,&nbsp;Yuze Li,&nbsp;Yuan Liu,&nbsp;Yi Nai,&nbsp;Wanli Hu,&nbsp;Guixiang Huang,&nbsp;Xinhua He","doi":"10.1016/j.plaphy.2024.109255","DOIUrl":"10.1016/j.plaphy.2024.109255","url":null,"abstract":"<div><div>The plant elongation factor eEF1A is involved in coregulating not only the translation of proteins and controlling translation-related signaling but also in signaling associated with cell growth, stress response and motility, controlling apoptosis and responding to adversity in plants. In this study, four eEF1A genes, namely, <em>ClEF1A-1</em>, <em>ClEF1A-2</em>, <em>ClEF1A-3</em> and <em>ClEF1A-4</em>, were identified from the genomic and ubiquitin-modified omics data of the 'Xiangshui Lemon', and bioinformatics analysis revealed that these four genes have relatively similar structures with conserved sequences; <em>ClEF1A-1</em> and <em>ClEF1A-4</em> were highly expressed in pollen, and temporal expression analysis demonstrated that the expression of <em>ClEF1A</em>s was significantly greater under self-pollination than under cross-pollination. All four genes were localized in the nucleus. <em>ClEF1A</em>s overexpression promoted early flowering and improved drought and salt stress tolerance in transgenic <em>Arabidopsis</em> plants. Yeast two-hybrid assays revealed that ClEF1As interacted with F-box, eIF3-G, the organ-specific-like protein S2, AGL62, S₁-RNase, S₂-RNase, S₃-RNase and S₄-RNase. This study demonstrated the functions of ClEF1As and provided a baseline for further studies on the associations of ClEF1As with self-incompatibility and abiotic stresses.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109255"},"PeriodicalIF":6.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging Arabidopsis roots exhibit hypersensitive gravitropism associated with distinctive auxin synthesis and polar transport within the elongation zone","authors":"Xinyu Li,&nbsp;Jiahui Liu,&nbsp;Ziwei Li,&nbsp;Ai Chen,&nbsp;Ruoxin Zhao,&nbsp;Shi Xu,&nbsp;Xianyong Sheng","doi":"10.1016/j.plaphy.2024.109257","DOIUrl":"10.1016/j.plaphy.2024.109257","url":null,"abstract":"<div><div>Gravitropism is crucial for plants to secure light, water, and minerals essential for developing seedlings. Despite its importance, the gravitropism of young roots remains largely unexplored. Herein, we reported that the emerging <em>Arabidopsis</em> roots exhibit hypersensitive gravitropism compared to mature roots, growing relatively slowly but bending exceptionally rapidly. This rapid gravibending is characterized by substantial growth inhibition and a distinctive auxin accumulation on the lower side of the elongation zone. Intriguingly, surgical experiments suggest that these auxins predominantly originate from the elongation zone rather than from the shoot or root cap. However, their asymmetrical distribution is heavily modulated by the root cap. Confocal analysis of GFP-tagged TAA1 further confirms that gravitational stimulus induces active auxin biosynthesis in the elongation zone of nascent roots but not in mature roots. Furthermore, mutations in the PIN proteins, especially PIN2, severely impair the rapid gravitropic responses in emerging roots. Interestingly, PIN2 in nascent roots is not confined to the epidermis and cortex but extends to the endodermis, contrasting with its distribution in mature roots. Gravitational stimulation leads to a marked asymmetrical distribution of PIN2 between the upper and lower sides of the roots, which is strongly inhibited by surgical removal of the root cap. These observations indicate that gravitational stimulation triggers active auxin synthesis and PIN protein-mediated lateral transport within the elongation zone of emerging roots, resulting in swift gravitropic responses. These results offer an intriguing enhancement and expansion to the mechanism of root gravitropism.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109257"},"PeriodicalIF":6.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of green and UVA spectra, and pre-harvest treatments on biomass and metabolite yields of indoor cultivated stevia rebaudiana","authors":"Narendren Rengasamy ,&nbsp;Rofina Yasmin Othman ,&nbsp;Hang Seng Che ,&nbsp;Jennifer Ann Harikrishna","doi":"10.1016/j.plaphy.2024.109252","DOIUrl":"10.1016/j.plaphy.2024.109252","url":null,"abstract":"<div><div><em>Stevia rebaudiana</em> is a high-value crop due to the strong commercial demand for its metabolites (steviol glycosides, SG) as an organic low-caloric sweetener with up to 300 times the sweetness of conventional sugar. Two experiments were conducted in this study. In the first experiment, treatments with varying green (GR1 &amp; GR2), UVA (UV1 &amp; UV2) and treatments that had both (UVGR1, UVGR2) were used. In the second experiment, separate set of plants were grown under base red-blue (RB) and natural sunlight before being transferred to GR2, UV2, UVGR2, and monochromatic light treatments of blue, green and UVA, for 3 and 10 days before harvest. RB and sunlight were used as the control for artificial and natural light respectively. Plants grown under the UVGR1 had the highest dry leaf biomass accumulation of 4.75 g plant⁻¹ (P &lt; 0.05), 458% and 660% higher than the RB (0.98 g plant⁻¹) and natural sunlight (0.72 g plant⁻¹) controls. UVA had the highest metabolite (Stevioside + Rebaudioside A) concentration of 27% (P &lt; 0.05) compared to the RB and sunlight controls at 17.24% and 15% respectively. The 10 day pre-harvest treatment with blue supplemented light yielded a dry biomass of 1.87 g plant^-1, a 190% increase compared to the RB control. However, the 3 day pre-harvest treatment had higher metabolite yields improvements compared to 10 day treatments with the highest yield obtained of 21.10% in 3-day pre-harvest irradiation that had supplemental UVA and blue light. UVGR1 was the most productive lighting strategy, resulting in the highest overall metabolite yield per plant.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109252"},"PeriodicalIF":6.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"From non-coding RNAs to histone modification: The epigenetic mechanisms in tomato fruit ripening and quality regulation\" [Plant Physiol. Biochem. 215 (2024), 109070].","authors":"Shengbo Chen, Jiazhen Han, Shu Wu, Shangjing Guo, Yufei Tang, Yujing Zheng, Lei Hu, Xingxing Zhang, Peng Zhang, Haifeng Zhang, Guodong Ren, Shuai Gao","doi":"10.1016/j.plaphy.2024.109102","DOIUrl":"10.1016/j.plaphy.2024.109102","url":null,"abstract":"","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":" ","pages":"109102"},"PeriodicalIF":6.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional stress in plants: Understanding sensing and resilience","authors":"Andrea Galatro,&nbsp;Susana Gallego,&nbsp;Carlos García-Mata,&nbsp;Ramiro Lascano,&nbsp;Guillermo E. Santa-María","doi":"10.1016/j.plaphy.2024.109207","DOIUrl":"10.1016/j.plaphy.2024.109207","url":null,"abstract":"","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"216 ","pages":"Article 109207"},"PeriodicalIF":6.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial to special issue on Photosynthetic organisms for sustainable development.","authors":"Giorgio Perin, Alexandra Dubini, Francesco Milano, Joanna Kargul, Maya Dimova Lambreva","doi":"10.1016/j.plaphy.2024.109263","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109263","url":null,"abstract":"","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":" ","pages":"109263"},"PeriodicalIF":6.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of foliar application of boron to alleviate cadmium toxicity in winter wheat (Triticum aestivum L.)","authors":"Mengchan Xie,&nbsp;Yifan Feng,&nbsp;Peng Zhao,&nbsp;Zhaojun Nie,&nbsp;Hongen Liu,&nbsp;Wei Gao,&nbsp;Chang Li,&nbsp;Fuqing Sui,&nbsp;Long Wang,&nbsp;Shiyu Qin (秦世玉)","doi":"10.1016/j.plaphy.2024.109264","DOIUrl":"10.1016/j.plaphy.2024.109264","url":null,"abstract":"<div><div>Our previous work shown that boron (B) can inhibit cadmium (Cd) absorption in winter wheat, while the mechanism is not well understood. In this study, the mechanism of foliage spray B on Cd uptake in Cd-contaminated farmland was characterized with three treatments: spraying distilled water (CK), spraying 0.3% B fertilizer (F0.3%), and spraying 0.6% B fertilizer (F0.6%). The spraying treatment was conducted during the jointing stage and booting stage, respectively. The objective was to investigate the impact of different concentrations of B fertilizer application on Cd content, yield, physiological biochemical response, Cd transporter in wheat. Results showed that the yield increased with increasing B spray concentration compared with CK treatment. The wheat yield increased by 1.29%∼12.50% under foliar B application. The Cd concentration in both shoot and root of wheat seedlings were significantly decreased with foliar application of B at jointing stage and booting stage. The Cd concentrations in root and seed were also decreased with B treatment at maturity stage, especially after treatment with F0.6%, the Cd absorption in wheat grains exhibited a significant reduction of 31.20%. In addition, foliar application of B significantly increased antioxidant enzyme activities, include APX, SOD, CAT, and POD. Compared with CK, the MDA content in leaves showed a significant decrease with 30.48%–50.14%, while the GSH content showed an increase with 76.32%–1.05%. The down-regulation of the uptake and transport genes (<em>TaNramp5</em>, <em>TaLCT1</em> and <em>TaHMA2</em>) and the up-regulation of compartmentalization transport genes (<em>TaTM20</em> and <em>TaHMA3</em>) may contribute to the reduction of Cd accumulation in shoot. Overall, our results suggest that foliar spraying of B could increase B accumulation and yield, and alleviate Cd toxicity by reducing Cd uptake, enhancing the antioxidant capacity, regulating the expression of Cd genes in wheat.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109264"},"PeriodicalIF":6.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: ROS and phytohormones: Two ancient chemical players in new roles","authors":"Vijay Pratap Singh,&nbsp;Durgesh Kumar Tripathi,&nbsp;José M. Palma,&nbsp;Francisco J. Corpas","doi":"10.1016/j.plaphy.2024.109149","DOIUrl":"10.1016/j.plaphy.2024.109149","url":null,"abstract":"","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"216 ","pages":"Article 109149"},"PeriodicalIF":6.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-time monitoring of stromal NADPH levels in Arabidopsis using a metagenome-derived NADPH-binding fluorescent protein","authors":"Jin Hoon Jang ,&nbsp;Da Been Kim ,&nbsp;Yeonsu Choi ,&nbsp;Roshanzadeh Amir ,&nbsp;Dae-Eun Cheong ,&nbsp;Hea-Jong Chung ,&nbsp;Sun-Hee Ahn ,&nbsp;Geun-Joong Kim ,&nbsp;Dong Wook Lee ,&nbsp;Ok Ran Lee ,&nbsp;Eung-Sam Kim","doi":"10.1016/j.plaphy.2024.109260","DOIUrl":"10.1016/j.plaphy.2024.109260","url":null,"abstract":"<div><div>The light irradiation to the plant chloroplasts drives NADPH and ATP synthesis in the stroma via the electron transport chains within the thylakoid membranes. Conventional methods for assessing photosynthetic light reactions are often invasive or require specific conditions. While detection markers do not significantly affect plant growth itself, developing a method for the real-time and non-invasive detection of NADPH is a highly impactful and important research area in plant physiology and biochemistry. This study introduces a genetically encoded NADPH-binding blue fluorescent protein (mBFP) targeted to the chloroplast stroma or thylakoid membrane in <em>Arabidopsis thaliana</em> and <em>Nicotiana benthamiana</em>. Using two-photon microscopy, we monitored real-time stromal NADPH levels in transgenic leaves of Arabidopsis in response to light exposure. A mutant mBFP construct targeted to the thylakoid membrane allowed us to detect the stromal NADPH levels in real time under different light conditions. This <em>in planta</em> biosensor provides a non-invasive tool for studying photosynthetic responses to light more quantitatively and holds potential for optimizing light conditions in controlled-environment agriculture, such as indoor vertical farms, to improve crop productivity.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109260"},"PeriodicalIF":6.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}