Plant Physiology and Biochemistry最新文献_第6页

Preinoculation with Bradyrhizobium japonicum enhances the salt tolerance of Glycine max seedlings by regulating polyamine metabolism in roots 通过调节根部的多胺代谢，预接种日本农杆菌可增强甘蓝幼苗的耐盐性

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-12 DOI: 10.1016/j.plaphy.2024.109196

{"title":"Preinoculation with Bradyrhizobium japonicum enhances the salt tolerance of Glycine max seedlings by regulating polyamine metabolism in roots","authors":"","doi":"10.1016/j.plaphy.2024.109196","DOIUrl":"10.1016/j.plaphy.2024.109196","url":null,"abstract":"<div><div>Rhizobia are common symbiotic microorganisms in the root system of leguminous plants that can usually provide nitrogen to the host through nitrogen fixation. Studies have shown that rhizobium-preinoculated soybean plants usually exhibit improved salt tolerance, but the underlying mechanism is not fully understood. In this paper, transcriptome sequencing (RNA-seq) revealed that preinoculation with rhizobia affected polyamine (PA) metabolism in soybean roots. The assay of PA contents showed that preinoculation with rhizobia significantly increased the putrescine (Put) content in roots and leaves during short-term salt treatment (0–5 d). Long-term salt treatment (5–7 d) resulted in a high Put content and significantly increased Spm and Spd contents, resulting in a rapid increase in the Put/(Spd + Spm) ratio (0–5 d) and subsequent decrease. Moreover, rhizobium preinoculation of soybean plants resulted in increased contents of conjugated and bound PAs under salt stress. Further transcriptome sequencing, PA contents, PA synthase expression and activity analysis revealed that <em>GmADC</em> may be a key gene related to salt tolerance in rhizobium-preinoculated soybean plants, and the <em>GmADC</em>-overexpressing soybean hairy-root composite plants exhibited less ROS damage, lower Cl<sup>−</sup>/NO<sub>3</sub><sup>−</sup> ratios and Na<sup>+</sup>/K<sup>+</sup> ratios, and stabilized ion homeostasis. Taken together, preinoculation with rhizobia increased the expression level and enzyme activity of arginine decarboxylase (ADC) in soybean roots, increased the content of Put in roots and leaves, and increased the content of conjugated and bound PAs in soybean plants, thereby alleviating the oxidative and ionic injuries of soybean plants and enhancing the salt tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution of the regulatory miR156-SPL9 module to the drought stress response in pigmented potato (Solanum tuberosum L.) 调控 miR156-SPL9 模块对色素马铃薯（Solanum tuberosum L.）干旱胁迫响应的贡献

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-12 DOI: 10.1016/j.plaphy.2024.109195

{"title":"Contribution of the regulatory miR156-SPL9 module to the drought stress response in pigmented potato (Solanum tuberosum L.)","authors":"","doi":"10.1016/j.plaphy.2024.109195","DOIUrl":"10.1016/j.plaphy.2024.109195","url":null,"abstract":"<div><div>Potato (<em>Solanum tuberosum</em> L.) is nowadays an important component of diversified cropping systems due to its adaptability, yielding capacity, and nutrition contribution. Breeding programs aiming at raising potato's nutritional value have mainly focused on the accumulation in potato tubers of health-promoting phytochemicals such as anthocyanins. In different plant species, increased amounts of anthocyanins in vegetative tissues have been associated with enhanced tolerance to abiotic and biotic stresses that challenge agrifood systems in the current context of global climate change. In the present study, we aimed at gaining insight into the effect of anthocyanin accumulation on the potato plants response to drought stress using three different potato genotypes with differential canopy and tuber pigmentation: the purple fleshed commercial variety Bleuet; the red fleshed breeding clone DAR170; and the non-pigmented commercial variety Monalisa. The varieties Bleuet and DAR170 exhibiting higher anthocyanin content in vegetative tissues than the Monalisa variety showed a remarkable inhibition of stem growth development under drought stress treatment suggestive of an anthocyanin-mediated physiological shift from growth to resilience as a mechanism of stress tolerance. The results of the expression analysis of stu-miR156a and its target <em>StSPL9</em> gene in the potato plants with different anthocyanin content, as well as their change in response to drought stress support the participation of the conserved miR156-SPL9 regulatory module in coordinating potato plants development and plant responses to drought stress, involving precise fine-tuning of anthocyanin biosynthesis.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

R2R3-MYB transcription factor GmMYB68 is involved in the accumulation of soybean isoflavones R2R3-MYB 转录因子 GmMYB68 参与大豆异黄酮的积累

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-12 DOI: 10.1016/j.plaphy.2024.109187

{"title":"R2R3-MYB transcription factor GmMYB68 is involved in the accumulation of soybean isoflavones","authors":"","doi":"10.1016/j.plaphy.2024.109187","DOIUrl":"10.1016/j.plaphy.2024.109187","url":null,"abstract":"<div><div>We aimed to investigate the regulatory function of the soybean transcription factor R2R3-MYB (<em>GmMYB68</em>) in isoflavone biosynthesis. Through comprehensive subcellular and chromosomal localization analyses, we found that <em>GmMYB68</em> was predominantly localized to the nucleus and mapped to chromosome Gm04. Notably, SSR markers near this gene significantly correlated with seed isoflavone content. <em>GmMYB68</em> overexpression markedly increased isoflavone contents, confirming its positive role in regulating isoflavone synthesis. <em>GmMYB68</em> also played a crucial role in the response of soybean to abiotic stress. Using RNA-seq and yeast one-hybrid techniques, we discovered an intricate interaction between <em>GmMYB68</em> and key isoflavone biosynthesis genes <em>GmCHS7</em> and <em>GmCHS8</em>. These findings provide novel insights into the mechanisms underlying isoflavone biosynthesis. Furthermore, using yeast two-hybrid experiments, we identified proteins interacting with <em>GmMYB68</em>, suggesting roles in the synthesis of physiologically active compounds and abiotic stress response. We not only elucidated the regulatory mechanisms of <em>GmMYB68</em> in isoflavone biosynthesis and abiotic stress response but also constructed a molecular network encompassing <em>GmMYB68</em>, <em>GmCHS7</em>, and <em>GmCHS8</em>. This network provides a theoretical basis for a better understanding of and strategies for improving soybean isoflavone biosynthesis.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutations in target gene confers resistance to acetolactate synthase inhibitors in Echinochloa phyllopogon 目标基因突变使 Echinochloa phyllopogon 对乙酰乳酸合成酶抑制剂产生抗性

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-11 DOI: 10.1016/j.plaphy.2024.109194

{"title":"Mutations in target gene confers resistance to acetolactate synthase inhibitors in Echinochloa phyllopogon","authors":"","doi":"10.1016/j.plaphy.2024.109194","DOIUrl":"10.1016/j.plaphy.2024.109194","url":null,"abstract":"<div><div><em>Echinochloa phyllopogon</em> is a noxious weed that can harm rice over prolonged periods. Recently, a penoxsulam-resistant variant of <em>E. phyllopogon</em> with a mutation in the acetolactate synthase (<em>ALS</em>) gene was collected in Northeastern China. In the present study, the molecular mechanism underlying herbicide resistance in mutant populations was evaluated. The GR<sub>50</sub> and IC<sub>50</sub> values of the herbicide-resistant mutant 1–11 were 27.0- and 21.4-fold higher than those of the susceptible population 2–31, respectively. In addition, pre-application of malathion reduced the GR<sub>50</sub> value of the resistant population. Additionally, mutant populations developed cross-resistance to other ALS inhibitors. <em>E. phyllopogon ALS</em> sequencing showed a Trp-574-Leu mutation in ALS2 variant 1–11. Molecular docking showed that the Trp-574-Leu substitution reduced the number of hydrogen bonds and altered the interaction between penoxsulam and ALS2. Transgenic <em>Arabidopsis</em> plants harboring the <em>ALS2</em> mutant gene also showed resistance to penoxsulam and other ALS inhibitors. Overall, our study demonstrated that the Trp-574-Leu mutation and P450-mediated metabolic resistance lead to the cross-resistance of <em>E. phyllopogon</em> to ALS inhibitors.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shading increases the susceptibility of alfalfa (Medicago sativa) to Pst. DC3000 by inhibiting the expression of MsIFS1 遮光会增加紫花苜蓿（Medicago sativa）对 Pst.DC3000 的敏感性。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-11 DOI: 10.1016/j.plaphy.2024.109191

{"title":"Shading increases the susceptibility of alfalfa (Medicago sativa) to Pst. DC3000 by inhibiting the expression of MsIFS1","authors":"","doi":"10.1016/j.plaphy.2024.109191","DOIUrl":"10.1016/j.plaphy.2024.109191","url":null,"abstract":"<div><div>Shade is a stressful factor for most plants, leading to both morphological and physiological changes, and often resulting in increased susceptibility to diseases and pathogen attacks. Our study revealed that the isoflavonoid synthesis pathway was inhibited in alfalfa under shade, resulting in a significant reduction in disease resistance. Overexpression of <em>MsIFS1</em>, a switch regulator in isoflavonoid synthesis, led to a notable increase in endogenous isoflavonoids and enhanced resistance to <em>Pseudomonas syringae pv</em>. tomato DC3000 (<em>Pst</em>. DC3000). Conversely, <em>MsIFS1</em>-RNAi had the opposite effect. Yeast one-hybrid (Y1H) assays revealed that the shade-responsive transcription factor MsWRKY41 could directly bind to the <em>MsIFS1</em> promoter. This interaction was confirmed through Dual-Luciferase Reporter (Dual-LUC) and Chromatin Immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) assays, both <em>in vitro</em> and <em>in vivo</em>. Overexpression of <em>MsWRKY41</em> not only enhanced alfalfa's resistance to <em>Pst</em>. DC3000 but also promoted the accumulation of isoflavonoids. Additionally, yeast two-hybrid (Y2H) assays showed that neither MsWRKY41 nor MsIFS1 physically interacted with the Type III effector (T3SE) HopZ1 secreted by <em>Pst</em>. DC3000, suggesting that the MsWRKY41-MsIFS1 module is not a direct target of HopZ1. These findings provide valuable theoretical insights and genetic resources for the development of shade-tolerant alfalfa with enhanced disease resistance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsCNGC7 modulates calcium dynamics and accelerates leaf senescence in rice OsCNGC7 调节钙动力学并加速水稻叶片衰老

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-11 DOI: 10.1016/j.plaphy.2024.109193

{"title":"OsCNGC7 modulates calcium dynamics and accelerates leaf senescence in rice","authors":"","doi":"10.1016/j.plaphy.2024.109193","DOIUrl":"10.1016/j.plaphy.2024.109193","url":null,"abstract":"<div><div>Calcium plays a crucial role in regulating plant senescence. However, the specific effects of increased intranuclear calcium versus cytoplasmic calcium on aging remain unclear. Cyclic nucleotide-gated channels (CNGCs), which manage Ca<sup>2</sup>⁺ levels in plant cells, are particularly significant in this context. These channels are known to relocate between the nuclear envelope and the plasma membrane in response to stress and developmental signals. Through this movement, CNGCs help regulate the balance of cytosolic and intranuclear Ca<sup>2</sup>⁺. In this study, we categorized the 16 CNGC genes in rice into five subgroups. <em>OsCNGCs</em> are notably expressed in leaves, especially during the reproductive stage. Both OsCNGC6 and OsCNGC7 exhibit dual localization to the plasma membrane and the nuclear envelope. Knockdown of <em>OsCNGC7</em> led to reduced levels of Ca<sup>2</sup>⁺ and K⁺ in plants. Conversely, yeast expressing the <em>OsCNGC7</em> gene showed increased sensitivity to Ca<sup>2</sup>⁺. Additionally, while the [Ca<sup>2</sup>⁺]<sub>cyt</sub> was maintained at relatively low levels in both wild-type and <em>OsCNGC7</em>-RNAi lines, the fluorescence intensity was significantly higher in <em>OsCNGC7</em>-overexpressing lines, particularly in the nucleus of root tips. Overexpression of <em>OsCNGC7</em> resulted in enhanced stomatal opening and accelerated leaf senescence from the tillering stage to grain filling in rice. Treatment with MeJA rapidly induced <em>OsCNGC7</em> expression, while knockdown of <em>OsCNGC7</em> delayed both MeJA-induced and dark-induced leaf senescence. Further analysis revealed that OsCNGC7 interacts with OsKAT2 and OsALMT2. In conclusion, our findings highlight the distinct roles of <em>OsCNGCs</em> in regulating senescence. This knowledge could provide new strategies for manipulating plant senescence and enhancing crop productivity.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional characterization of the diterpene synthase family in Pogostemon cablin (Blanco) Benth Pogostemon cablin (Blanco) Benth.中二萜合成酶家族的鉴定和功能表征

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-10 DOI: 10.1016/j.plaphy.2024.109190

{"title":"Identification and functional characterization of the diterpene synthase family in Pogostemon cablin (Blanco) Benth","authors":"","doi":"10.1016/j.plaphy.2024.109190","DOIUrl":"10.1016/j.plaphy.2024.109190","url":null,"abstract":"<div><div><em>Pogostemon cablin</em> (Blanco) Benth (Patchouli) is an aromatic herb extensively used in pharmaceutical and cosmetic industries. Sesquiterpenes are the characteristic constitutes in patchouli which are synthesized in the glandular trichomes on leaves and stems. Gibberellic acid (GA), a tetracyclic diterpenoid, plays a crucial role in the formation of glandular trichome. However, the diterpene biosynthesis remains largely unknown in patchouli. Here we identified a small diterpene synthases (diTPSs) family comprising three class II diTPSs (PatCPS1-3) and three class I diTPSs (PatKSL1 and PatGLS1-2). These diTPSs are functionally characterized using a yeast heterologous expression system. PatCPS1 was identified as an <em>ent</em>-copalyl diphosphate synthase (<em>ent</em>-CPS), in combination with PatKSL1, yield <em>ent</em>-kaurene, the precursor of GA, indicating their involvement in primary metabolism. PatCPS2 converted GGPP into (+)-8, 13-copalyl diphosphate (CPP). No activity was detected for PatCPS3, PatGLS1 and PatGLS2. Three ohnologs of <em>PatCPS1</em> were further characterized to explore the possible functional differentiation of <em>ent</em>-CPS during the evolution of tetraploid hybrid patchouli genome. GC-MS analysis showed all ohnologs are functional <em>ent</em>-CPSs, demonstrating the functional conservation of PatCPS1 during evolution. Expression profiling by qRT-PCR showed <em>PatCPS1</em> and <em>PatKSL1</em> are ubiquitously expressed in all tissues, consistent with their involvement in primary metabolism. Conversely, <em>PatCPS2</em> and <em>PatCPS3</em> were predominantly expressed in the above ground parts, indicating a role in specialized metabolism. In summary, these findings clarify the early stages of GA biosynthesis in patchouli and provide gene elements for further metabolic engineering of sesquiterpenes via diterpenoids.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification and expression analysis of SlKFB gene family (Solanum lycopersicum) and the molecular mechanism of SlKFB16 and SlKFB34 under drought SlKFB基因家族（Solanum lycopersicum）的全基因组鉴定和表达分析以及SlKFB16和SlKFB34在干旱条件下的分子机制

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-10 DOI: 10.1016/j.plaphy.2024.109192

{"title":"Genome-wide identification and expression analysis of SlKFB gene family (Solanum lycopersicum) and the molecular mechanism of SlKFB16 and SlKFB34 under drought","authors":"","doi":"10.1016/j.plaphy.2024.109192","DOIUrl":"10.1016/j.plaphy.2024.109192","url":null,"abstract":"<div><div>Environmental stress significantly affects plant growth and productivity. The effects of drought stress on plants are reflected primarily in enzyme activity, membrane systems, and cell-water loss. Here, the Kelch repeat F-box (KFB) protein family in tomato was systematically identified and analysed. Using bioinformatics, we identified 37 <em>SlKFB</em> family members in the tomato genome and analysed their protein structure, phylogenetic relationships, chromosome distribution, and expression under drought or biotic-stress conditions. Transcriptome data revealed that <em>SlKFB</em> members exhibit differential responses to drought stress, with significant differences in <em>SlKFB16</em> and <em>SlKFB34</em> expression. Functional analysis revealed that <em>SlKFB16</em> functions in the cytoplasm and <em>SlKFB34</em> in the nucleus and cytoplasm. Under drought stress, <em>SlKFB16</em> and <em>SlKFB34</em>-silencing significantly reduced reactive oxygen species scavenging and resistance to drought stress. These findings provide a reference for further studies of the mechanisms of <em>SlKFB16</em> and <em>SlKFB34</em> in drought stress in tomato as well as a foundation for enhancing their resistance to drought stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined transcriptome and physiological analysis reveals exogenous sucrose enhances photosynthesis and source capacity in foxtail millet 转录组和生理分析相结合揭示外源蔗糖可提高狐尾黍的光合作用和源能力

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-10 DOI: 10.1016/j.plaphy.2024.109189

{"title":"Combined transcriptome and physiological analysis reveals exogenous sucrose enhances photosynthesis and source capacity in foxtail millet","authors":"","doi":"10.1016/j.plaphy.2024.109189","DOIUrl":"10.1016/j.plaphy.2024.109189","url":null,"abstract":"<div><div>Foxtail millet (<em>Setaria italica</em> (L.) P. Beauv.) is an environmentally friendly crop that meets the current requirements of international food security and is widely accepted as a photosynthesis research model. However, whether exogenous sucrose treatment has a positive effect on foxtail millet growth remains unknown. Here, we employed physiological and molecular approaches to identify photosynthesis and source capacity associated with exogenous sucrose during the growth of Jingu 21 seedlings. RNA-seq analysis showed that some differentially expressed genes (DEGs) related to photosynthesis and carotenoid biosynthesis were induced by exogenous sucrose and that most of these genes were up-regulated. An increase in gas exchange parameters, chlorophyll content, and chlorophyll fluorescence of Jingu 21 was noted after exogenous sucrose addition. Furthermore, exogenous sucrose up-regulated genes encoding sucrose and hexose transporters and enhanced starch and sucrose metabolism. More DEGs were up-regulated by sucrose, the nonstructural carbohydrate (NSC) content in the leaves increased and energy metabolism and sucrose loading subsequently improved, ultimately enhancing photosynthesis under normal and dark conditions. Further analysis revealed that WRKYs, ERFs, HY5, RAP2, and ABI5 could be key transcription factors involved in growth regulation. These results indicate that exogenous sucrose affects the normal photosynthetic performance of foxtail millet by increasing NSC transport and loading. They improve our understanding of the molecular mechanisms of the effects of exogenous sucrose on photosynthesis in foxtail millet, providing an effective measure to enhance source–sink relationships and improve yield.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome wide analysis of HMA gene family in Hydrangea macrophylla and characterization of HmHMA2 in response to aluminum stress 大绣球花 HMA 基因家族的全基因组分析及 HmHMA2 对铝胁迫的响应特征

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-09 DOI: 10.1016/j.plaphy.2024.109182

{"title":"Genome wide analysis of HMA gene family in Hydrangea macrophylla and characterization of HmHMA2 in response to aluminum stress","authors":"","doi":"10.1016/j.plaphy.2024.109182","DOIUrl":"10.1016/j.plaphy.2024.109182","url":null,"abstract":"<div><div>Aluminum toxicity poses a significant threat to plant growth, especially in acidic soils. Heavy metal ATPases (HMAs) are crucial for transporting heavy metal ions across plant cell membranes, yet their role in Al<sup>3+</sup> transport remains unexplored. This study identified eight <em>HmHMA</em> genes in the genome of Hydrangea macrophylla, categorizing them into two major clades based on phylogenetic relationships. These genes were found unevenly distributed across six chromosomes. Detailed analysis of their physicochemical properties, collinearity, and gene structure was conducted. RNA-seq and qRT-PCR analyses revealed that specific <em>HmHMA</em> genes, notably HmHMA2, were predominantly expressed in roots and flowers under Al<sup>3+</sup> stress, indicating their potential role in Al<sup>3+</sup> tolerance. HmHMA2 showed significant expression in roots, especially under Al<sup>3+</sup> stress conditions, and when expressed in yeast cells, it conferred resistance to aluminum and zinc but increased sensitivity to cadmium. Overexpression of HmHMA2 in hydrangea leaf discs significantly improved Al<sup>3+</sup> tolerance, reduced oxidative stress markers like hydrogen peroxide and malondialdehyde, and enhanced antioxidant enzyme activity such as SOD, POD and CAT compared to controls. These findings shed lights on the potential role of HmHMAs in Al transport and tolerance in H. macrophylla.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0