Plant Physiology and Biochemistry最新文献

{"title":"Integrated analyses of the transcriptome and metabolome revealed the coloring mechanism of red-pericarp wampee (Clausena lansium)","authors":"Shujun Peng,&nbsp;Xiaoyue Zhu,&nbsp;Jiaxuan Chen,&nbsp;Jiayi Chen,&nbsp;Xinglong Hu,&nbsp;Jiale Wu,&nbsp;Zhike Zhang,&nbsp;Jietang Zhao,&nbsp;Guibing Hu,&nbsp;Irfan Ali Sabir,&nbsp;Yonghua Qin","doi":"10.1016/j.plaphy.2025.109959","DOIUrl":"10.1016/j.plaphy.2025.109959","url":null,"abstract":"<div><div>Wampee (<em>Clausena lansium</em>), a tropical evergreen fruit from the Rutaceae family renowned for its rich nutrient profile and bioactive compounds, presents a fascinating case study in fruit coloration. However, changes in anthocyanins, and expressions of metabolism-associated genes during fruit maturation of red-pericarp wampee (‘ZR’) are not documented. In this study, metabolic and gene expression profiles of anthocyanin across different fruit developmental stages of red and yellow-pericarp wampees were analyzed. A total of 38 distinct anthocyanins were identified from the comparison of ‘ZR3’ and ‘JX3’ wampees and categorized into 17 differential anthocyanin metabolites (DAMs). Among these DAMs, fifteen were up-regulated in ‘ZR3’, while two were down-regulated compared with ‘JX3’. The delphinidin 3-[6-(4-(caffeoylrhamnosyl)glucoside)] was the predominant anthocyanins in ‘ZR’ wampee. A total of 1135 metabolics mainly including amino acid metabolites and flavonoids were detected in the ‘ZR’ and ‘JX’ wampees. Significant differences were mainly concentrated in the biosynthesis of secondary metabolites in terms of flavonoid biosynthesis, ABC transporters, and anthocyanin biosynthesis. According to the combined analyses of qRT-PCR and transcriptome, the transcript levels of <em>PAL1</em>, <em>PAL2</em>, <em>CHS1</em> and <em>UFGT1</em> in ‘ZR’ wampee were two to eight-fold higher than those in ‘JX’ wampee during fruit pigmentation. Our study offers valuable insights into the mechanisms of anthocyanin accumulation in the red pericarp of wampee which is helpful to regulate fruit coloration of wampee.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109959"},"PeriodicalIF":6.1,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CAM5, WRKY53, and TGA5 regulate defense gene expression mediated by the volatile organic compound ethyl vinyl ketone in Arabidopsis","authors":"Junqing Gong ,&nbsp;Jinchao Han ,&nbsp;Xinyi Chen ,&nbsp;Chunyang Jiao ,&nbsp;Mian Zhang","doi":"10.1016/j.plaphy.2025.109952","DOIUrl":"10.1016/j.plaphy.2025.109952","url":null,"abstract":"<div><div>Plants produce ethyl vinyl ketone (evk) in response to biotic stress; however, the downstream defense responses triggered by evk remain unclear. Our research utilizing reverse-transcription quantitative PCR showed that evk treatment antagonized the effects of salicylic acid (SA) and jasmonic acid (JA) and that CALMODULIN 5 (CAM5) positively regulated <em>WRKY53</em> expression, which, in turn, positively regulated the expression of SA-related genes. Additionally, the GST-pulldown assay, yeast two-hybrid assays, and luciferase complementation test all suggested that CAM5 and WRKY53 physically interacted and formed a complex that was unlocked by Ca²⁺ to release WRKY53. Electrophoretic mobility shift and dual-luciferase reporter assays demonstrated that WRKY53 enhanced the binding of TGA5 to the <em>PATHOGENESIS-RELATED 1</em> (<em>PR1</em>) promoter, thereby enhancing the expression of the defense gene <em>PR1</em>. Conversely, WRKY53 diminishes the binding of TGA5 to the PLANT DEFENSIN 1.2 (<em>PDF1.2</em>) promoter and inhibits <em>PDF1.2</em> expression. As the expression of <em>WRKY53</em> decreases in later stages, this inhibitory effect weakens, allowing TGA5 to positively activate <em>PDF1.2</em> expression. In this study, we propose a framework in which evk, as a resistance-inducing substance, facilitates the plant’s ‘rescue' through the regulation of defense-related gene expressions in Arabidopsis.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109952"},"PeriodicalIF":6.1,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and characterization of CONSTANS-like transcription factors reveal that three CsCOLs regulate the cannabinoid biosynthesis in Cannabis","authors":"Maolun Gao ,&nbsp;Shanshan Chen ,&nbsp;Lingzhe Kong ,&nbsp;Liwei Wang ,&nbsp;Xiangxiao Meng ,&nbsp;Ziyan Xie ,&nbsp;Zhichao Xu ,&nbsp;Yaolei Mi","doi":"10.1016/j.plaphy.2025.109942","DOIUrl":"10.1016/j.plaphy.2025.109942","url":null,"abstract":"<div><div>Cannabis (Cannabis <em>sativa</em> L.) has been cultivated as a versatile industrial crop for millennia, serving for food, fiber, and medicine. Cannabinoids are characteristic medicinal active compounds in cannabis, mainly being rich in female inflorescences. CONSTANS-like (COL) transcription factors are primarily involved in the photoperiod process of flowering plants. However, knowledge about their regulatory mechanism for secondary metabolites is limited. Eleven CsCOLs were identified from the cannabis genome based on the phylogenetic relationship and conserved domains in this study. The number of CsCOLs in cannabis showed apparent contraction and their functional divergence. <em>CsCOL1, CsCOL5,</em> and <em>CsCOL7</em> exhibited high expression in flowers and bracts and their overexpression elevated the content of CBDA and CBGA by promoting the expression of related structural genes involved in cannabinoid biosynthesis. In addition, CsCOL7 was bound to the promoters of four structural genes, including <em>CsAAE</em>, <em>CsOLS</em>, <em>CsPT4</em>, and <em>CsCBDAS</em>, to regulate their gene expression in the manner of repressing the upstream and activating the downstream genes. CsCOL1 positively regulated the expression of <em>CsPT4</em> and <em>CsCBDAS</em>. In addition, CsCOL5 positively regulated the expression of <em>CsOLS</em> and <em>CsPT4</em> via binding of their promoters. Here, we explored the potential transcription regulation mechanism of CsCOLs in cannabinoid biosynthesis in <em>C. sativa</em> for the first time, even though a more profound investigation should be conducted in cannabis plants in the future. The findings expanded the knowledge of COLs in regulating secondary metabolites and provided insights into CsCOLs in cannabinoid biosynthesis.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109942"},"PeriodicalIF":6.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships between leaf morphologies and stomatal traits of Achnatherum splendens under different soil moistures in semiarid Northern China","authors":"Yuting Shen ,&nbsp;Yuxuan Liu ,&nbsp;Yang Gao ,&nbsp;Yuhan Liu ,&nbsp;Tianxiao Ma ,&nbsp;Chunwang Xiao","doi":"10.1016/j.plaphy.2025.109947","DOIUrl":"10.1016/j.plaphy.2025.109947","url":null,"abstract":"<div><div>The interplay between leaf and stomata is fundamental for the exchange of carbon and water between plants and the atmosphere. This relationship is pivotal for comprehending the connections between the physical structure and physiological metabolism of plant leaves under various conditions. A study examining the relationship between leaf morphological traits and stomatal traits of the <em>Achnatherum splendens</em> under varying natural soil moisture conditions was conducted along the southern shore of Hulun Lake in Inner Mongolia. In our study, stomatal density (SD) and stomatal relative area (SRA) significantly decreased, while stomatal width (SW), leaf length (LL), leaf area (LA) and leaf dry weight (LDW) increased correspondingly with rising soil moisture. A significant positive correlation between the stomatal traits and leaf morphological traits was observed through principal component analysis (PCA) and permutation test, indicating that the functional traits of leaf morphologies and stomata of <em>A</em>. <em>splendens</em> exhibit a positive relationship. The results reveal the resource utilization strategies of <em>A</em>. <em>splendens</em> in response to varying soil water conditions, offering a scientific data foundation and theoretical support for the management of <em>A</em>. <em>splendens</em> grassland, ecological restoration, and the strategic planning of animal husbandry in Inner Mongolia.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109947"},"PeriodicalIF":6.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of PcHD8 from Pogostemon cablin related to the regulation of trichome development","authors":"Xiaoqi Peng ,&nbsp;Jin Huang ,&nbsp;Yaru Lu ,&nbsp;Yingying Liang ,&nbsp;Lu Yang ,&nbsp;Yuwei Zhang ,&nbsp;Qixuan Huang ,&nbsp;Huili Lai ,&nbsp;Wenru Wu","doi":"10.1016/j.plaphy.2025.109944","DOIUrl":"10.1016/j.plaphy.2025.109944","url":null,"abstract":"<div><div><em>Pogostemon cablin</em>, a herbaceous plant of the <em>Lamiaceae</em> family, is widely recognized for the medicinal and industrial applications of its dried aerial parts. The plant's surface is densely populated with trichomes, which are believed to be the primary sites for the biosynthesis and accumulation of patchouli essential oil. However, the molecular mechanisms underlying the development of these trichomes in <em>P</em>. <em>cablin</em> remain largely unexplored. This study employed transcriptomic sequencing to identify and characterize genes co-regulated in trichome development and essential oil biosynthesis in <em>P. cablin</em>. Notably, we report the first identification of <em>PcHD8</em>, a member of the HD-ZIP gene family. Homologous genes, such as <em>AaHD8</em> in <em>Artemisia annua</em> and <em>SlHD8</em> in <em>Solanum lycopersicum</em>, have been shown to promote both trichome formation and secondary metabolite synthesis. We cloned <em>PcHD8</em> and overexpressed it in <em>Nicotiana tabacum</em>, which resulted in a significant increase in trichome density compared to the control. Conversely, silencing <em>PcHD8</em> via virus-induced gene silencing (VIGS) in <em>P. cablin</em> led to a marked reduction in trichome density. GC-MS analysis revealed a significant decline in the key biomarkers of patchouli essential oil, patchouli alcohol and pogostone, following <em>PcHD8</em> silencing. These findings establish <em>PcHD8</em> as a key positive regulator of trichome development and essential oil biosynthesis. This study offers critical insights into the genetic regulation of plant traits affecting medicinal quality. Our findings highlight <em>PcHD8</em> as a promising candidate gene for molecular breeding in <em>P. cablin</em> and may provide a reference for research on other trichome-rich, volatile oil-producing plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109944"},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of SWEET gene family in passion fruit reveals the involvement of PeSWEET3 in soluble sugar accumulation","authors":"Rui Ren ,&nbsp;Yuxuan Chen ,&nbsp;Xiao Yu ,&nbsp;Xianrui Peng ,&nbsp;Lihui Zeng ,&nbsp;Ting Fang","doi":"10.1016/j.plaphy.2025.109943","DOIUrl":"10.1016/j.plaphy.2025.109943","url":null,"abstract":"<div><div>The sugars will eventually be exported transporters (SWEET) family is a class of sugar transporters that play crucial roles in plant growth, reproduction, and stresses response. The characteristics of 21 <em>PeSWEETs</em>, identified in passion fruit (<em>Passiflora edulis</em> Sims) and divided into four clades, were evaluated. Structural feature analysis showed that exon numbers ranged from 4 to 12, while motif 1, 2, and 3 were highly conserved. Analysis of RNA-seq from different tissues revealed that <em>PeSWEETs</em> showed tissue-specificity. Additionally, <em>Cis</em>-acting regulatory element analysis indicated that abundant hormone-response and stress response elements were enriched in promoters of <em>PeSWEETs</em>. Exogenous abscisic acid (ABA) treatment led to an increase in the soluble sugar accumulation and up-regulated the expression level of <em>PeSWEET3</em>. The PeSWEET3 protein localizes on the plasma membrane and exhibits transport activity of fructose, glucose, and mannose. Furthermore, ectopic expression of <em>PeSWEET3</em> significantly enhanced soluble sugar accumulation in leaves of transgenic tobacco. Collectively, these results lay a solid foundation for further exploration on the role of <em>PeSWEETs</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109943"},"PeriodicalIF":6.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel wheat S1-bZIP gene, TabZIP11-D, confers stress resistance in Arabidopsis","authors":"Lina Zhang ,&nbsp;Zhen Yu ,&nbsp;Xingyan Liu ,&nbsp;Yaoyao Wang ,&nbsp;Jing Luo ,&nbsp;Yinghong Wang ,&nbsp;Ning Yang ,&nbsp;Jie Du ,&nbsp;Lan Ding ,&nbsp;Chuan Xia ,&nbsp;Lichao Zhang ,&nbsp;Xiuying Kong","doi":"10.1016/j.plaphy.2025.109946","DOIUrl":"10.1016/j.plaphy.2025.109946","url":null,"abstract":"<div><div>Most subgroup S1 basic leucine zipper (bZIP) transcription factors (TFs) play a crucial role in the abiotic stress responses. However, their functions and molecular mechanisms remain poorly characterized in wheat (<em>Triticum aestivum</em> L.). In this study, we identified a novel subgroup S1 bZIP gene, designated <em>TabZIP11-D</em>, which was transcriptionally responsive to abscisic acid (ABA), salt, and cold stresses. <em>TabZIP11-D</em> encodes a nuclear-localized protein that lacks transcriptional activation activity in yeast. The Ca²⁺ blocker LaCl₃ significantly suppressed the salt-induced expression of <em>TabZIP11-D</em>. TabZIP11-D interacted with the Ca²⁺-dependent protein kinases (TaCDPK1, TaCDPK5, TaCDPK9-1, and TaCDPK30) and the CBL-interacting protein kinase TaCIPK31. Overexpression of <em>TabZIP11-D</em> enhanced salt and freezing tolerance by modulating soluble sugar and proline accumulation, reducing hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) contents, and regulating the expression levels of stress-responsive genes. Furthermore, TabZIP11-D formed a homodimer with itself and heterodimers with group C bZIP proteins. Modified yeast one-hybrid assays revealed that TabZIP14 and TabZIP36 significantly enhanced TabZIP11-D binding to the G-box <em>cis</em>-element in the promoter region of <em>TaCBF1</em> gene. These findings demonstrate that TabZIP11-D heterodimerizes with TabZIP14/36 to regulate cold signaling by promoting the <em>TaCBF1</em> transcription. TabZIP11-D functions as a positive regulator in the salt stress response by interacting with TaCDPK1/5/9-1/30 and TaCIPK31.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109946"},"PeriodicalIF":6.1,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endogenous salicylic acid contributes to cadmium tolerance in Monochoria korsakowii through upregulation of photosynthetic efficiency, antioxidant capacity, and chelators accumulation","authors":"Qianqian Zheng ,&nbsp;Lu Yang ,&nbsp;Jianpan Xin,&nbsp;Chu Zhao,&nbsp;Yan Li,&nbsp;Runan Tian","doi":"10.1016/j.plaphy.2025.109940","DOIUrl":"10.1016/j.plaphy.2025.109940","url":null,"abstract":"<div><div>Exogenous salicylic acid (SA) enhances plant tolerance to cadmium (Cd) stress by preserving chlorophyll, stabilizing osmoprotectants, and upregulating antioxidant activity alongside the ASA-GSH system. However, the role of endogenous SA in plant tolerance to Cd stress remains poorly understood. Therefore, we cultivated <em>Monochoria korsakowii</em> hydroponically and sprayed the SA biosynthesis inhibitors (2-aminoindane-2-phosphonic acid and 1-aminobenzotriazole) in an attempt to explore the correlation between endogenous SA and other Cd tolerance mechanisms. Compared with control, 0.3 mM Cd treatment induced reductions of net photosynthetic rate (<em>Pn</em>), total chlorophyll (T Chl), catalase (CAT), and soluble protein (SP), while malondialdehyde increased. To mitigate Cd toxicity, <em>M. korsakowii</em> upregulated peroxidase (POD), superoxide dismutase (SOD), glutathione reductase (GR), ascorbic acid (ASA), nonprotein thiols (NPT), phytochelatin (PC), and proline. High concentrations of SA inhibitors exacerbated Cd-induced oxidative damage and suppressed these tolerance mechanisms. Compared with T4, T6 plants exhibited marked reductions in <em>Pn</em>, T Chl, CAT, POD, SOD, GSH, GR, ASA, ascorbate peroxidase, NPT, PCs, SP, and translocation factors. Concurrently, T6 plants sprayed with SA inhibitors exhibited suppressed SA, methyl salicylate, and zeatin accumulation, contrasting with heightened jasmonic acid and abscisic acid concentrations. We propose that endogenous SA is crucial for preserving the photosynthetic apparatus, activating the antioxidant system, and promoting the accumulation of chelators and SP in <em>M. korsakowii</em> under Cd stress. Furthermore, endogenous SA may function synergistically with methyl salicylate and zeatin to regulate plant physiological responses to Cd. This study provides valuable insights into the Cd tolerance mechanisms in <em>M. korsakowii</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109940"},"PeriodicalIF":6.1,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Type-dependent biostimulant-mediated induction of nutrients and phenolic compounds improved plant tolerance against canola aphids (Brevicoryne brassicae L.)","authors":"Muhammad Wajid Javed ,&nbsp;Mansoor-ul-Hasan ,&nbsp;Muhammad Sagheer ,&nbsp;Shahbaz Talib Sahi","doi":"10.1016/j.plaphy.2025.109930","DOIUrl":"10.1016/j.plaphy.2025.109930","url":null,"abstract":"<div><div>The canola aphid (<em>Brevicoryne brassicae</em> L.) is a global threat to the production of canola. Field studies on the mechanism of biostimulants-driven plant tolerance against canola aphids are either scare or inconclusive. To assess the potential of biostimulants—salicylic acid (SA) and citric acid (CA)—in conferring induced resistance against canola aphids, two-year field experiments were conducted. The 0, 0.5, and 1 mM concentrations of each biostimulant were compared to an insecticide (Carbosulfan 20 EC) and infested control groups. Aphid abundance decreased by 19.3 % (1 mM SA), 16.3 % (0.5 mM SA), and 15.7 % (1 mM CA), compared to the insecticide treatment. Significant increases were observed in plant height, number of siliques (pods), seeds per silique, thousand-seed weight, seed yield m⁻², total yield (kg ha⁻¹), and oil contents. The 1 mM SA led to the highest levels of nitrogen, phosphorous, magnesium, calcium, and zinc, while 1 mM CA had highest concentrations of potassium, iron, and copper. Reverse-phase HPLC (<em>RP</em>-HPLC) separation analyses revealed that 1 mM SA promoted the accumulation of gallic, m-coumaric, p-coumaric, and cinnamic acid, while 1 mM CA induced the synthesis of myricetin, quercetin, syringic, vanillic, caffeic, chlorogenic, ferulic, and sinapic acid. The 0.5 mM concentration of either biostimulant showed intermediate efficacy. Our findings demonstrate that each biostimulant induces a distinct nutrient and phenolic compound in canola likely through a unique defense eliciting mechanism. These insights advances our understanding of the aphid-stress tolerance mechanism in canola to support the breeding of aphid-resistant varieties.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109930"},"PeriodicalIF":6.1,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complex regulatory network of ZmbZIP54-mediated Pb tolerance in maize","authors":"Fengxia Hou ,&nbsp;Yuru Liang ,&nbsp;Mengxiang Sang ,&nbsp;Guixiang Zhao ,&nbsp;Jing Song ,&nbsp;Peng Liu ,&nbsp;Chaoying Zou ,&nbsp;Zhong Chen ,&nbsp;Langlang Ma ,&nbsp;Yaou Shen","doi":"10.1016/j.plaphy.2025.109945","DOIUrl":"10.1016/j.plaphy.2025.109945","url":null,"abstract":"<div><div>Lead (Pb) is highly toxic and widely distributed in the soil, causing adverse effects on plant growth and yield formation. Herein, the combination of transmission electron microscope (TEM), energy dispersive X-ray Spectroscopy (EDS), and comparative transcriptome analyses was conducted to reveal the cytological mechanism and regulatory network of in the <em>ZmbZIP54-</em>mediated Pb tolerance in maize. As results, Z<em>mbZIP54</em> helped in Pb²⁺ retention in the cell wall and intercellular space, inhibiting Pb²⁺ entering the cells and reducing its toxic effects on cell ultrastructure. Meanwhile, <em>ZmbZIP54</em> was involved in the transition between the HCl-extracted and CH₃COOH-extracted Pb speciations. At the molecular level, <em>ZmbZIP54</em> affected the macromolecule metabolism, thus decreasing Pb accumulation in the roots. Moreover, <em>ZmZIFL1</em> and <em>NRT1/PTR</em> were the direct targets of <em>ZmbZIP54,</em> which participated in heavy metal binding, nitrogen uptaking, and IAA transport and thus mediated Pb transport, Pb speciation transition, and antioxidant enzyme activation. Collectively, we proposed a model to explain the complex regulatory network mediated by <em>ZmbZIP54</em> and its target genes in maize tolerance to Pb stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109945"},"PeriodicalIF":6.1,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}