Plant Physiology and Biochemistry最新文献

{"title":"Galactinol synthase 4 influences plant height by affecting phenylpropanoid metabolism and the balance of soluble carbohydrates in tomato.","authors":"Shuo Wang, Peijie He, Zhijun Wang, Huidong Zhang, Sida Meng, Mingfang Qi","doi":"10.1016/j.plaphy.2025.109484","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109484","url":null,"abstract":"<p><p>Plant height is a key trait that significantly influences plant architecture, disease resistance, adaptability to mechanical cultivation, and overall economic yield. Galactinol synthase (GolS) is a crucial enzyme involved in the biosynthesis of raffinose family oligosaccharides (RFOs). It plays a significant role in carbohydrate transport and storage, combating abiotic and biotic stresses, and regulating plant growth and development. The present study employed CRISPR/Cas9 gene-editing technology to create the gols4 mutant in tomato (Solanum lycopersicum), which exhibits a semi-dwarf phenotype. Results showed that glucose, sucrose, myo-inositol, galactinol, and raffinose levels were significantly reduced in the slgols4 mutant, impairing material transport and affecting the balance of soluble carbohydrates. Integration of transcriptomics and metabolomics data indicated not only a decrease in the expression of synthesis genes related to phenylpropanoid biosynthesis but also a significant reduction in the content of lignin and flavonoids, which are byproducts of phenylpropanoid metabolism. This may be a key factor contributing to dwarfism. Overall, these findings provide evidence for the role of SlGolS4 in regulating sugar metabolism and phenylpropanoid metabolism, offering new insights into tomato dwarfing cultivation and germplasm resources.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109484"},"PeriodicalIF":6.1,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ACC treatment induced alterations in flavonoid accumulation in Toxicodendron vernicifluum.","authors":"Xiaomin Ge, Aiguo Zhao, Shasha Li, Xiao Zhang, Huiying Shang, Chen Chen, Guoqing Bai","doi":"10.1016/j.plaphy.2025.109491","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109491","url":null,"abstract":"<p><p>Lacquer tree (Toxicodendron vernicifluum) is an important economic crop and is rich in flavonoids. ACC (1-aminocyclopropane-1-carboxylic acid) is the precursor to ethylene. ACC treatment can induce physiological and biochemical responses in plants. In this study, flavonoids in the leaves of Toxicodendron vernicifluum, treated with ACC at different time points, were identified and analyzed. A total of 83 flavonoids were identified, including 38 flavonoids and 45 flavonoid glycosides. Among these, 48 flavonoids were first reported in T. vernicifluum. The total flavonoid content significantly increased following ACC treatment, although the accumulation patterns of individual flavonoids varied. Flavonoids with similar structure or glycosylation/acetylation modifications exhibited a positive correlation in their content changes in T. vernicifluum leaves under ACC treatment. Transcriptome sequencing was conducted on control and 3-days ACC-treated leaves, revealing an upregulation in the expression of genes related to flavonoids biosynthesis, such as PAL, CCR, CHS, MYB and ERF encoding genes. We hypothesized that ACC regulated flavonoids biosynthesis by activating ERF and MYB transcription factors in the ethylene signaling pathway. This study provided evidence for the regulation of flavonoids biosynthesis in lacquer trees through ACC treatment.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109491"},"PeriodicalIF":6.1,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated temperature has more pronounced effect on anthesis tomato plant than cadmium stress and reduced nitrogen supply.","authors":"Rong Zhou, Xiaqing Yu, Yankai Li, Yanhai Ji, Xiaoming Song, Hanne Lakkenborg Kristensen, Carl-Otto Ottosen, Fangling Jiang, Zhen Wu","doi":"10.1016/j.plaphy.2025.109498","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109498","url":null,"abstract":"<p><p>Plants are often exposed to combined stress, e.g. heat and cadmium (Cd) stress under natural conditions. Nitrogen (N) fertilizer is usually applied in excess, even though it is an essential nutrition for plants. We aimed to clarify the effects of elevated temperature, Cd stress, reduced N fertilizer and their interaction on leaf physiology and metabolism of anthesis tomato plants. Tomato plants at anthesis stage were subjected to unique combinations of elevated temperature (34 °C/30 °C), Cd stress (0.1 mM CdCl₂) and half N (N = 95 ppm) treatment. The elevated temperature generally decreased leaf intracellular CO₂ concentration and stomatal conductance, but increased transpiration rate with no significant changes in net photosynthetic rate, as compared with control. The plants under elevated temperature exhibited higher chlorophyll content as well as lower anthocyanin than under control temperature. The temperature had significant impacts on open flowers in the 1st inflorescence counting from bottom, open flower percentage in the 1st inflorescence, fresh and dry weight of flowers. Temperature played a predominant role in the changes of metabolites among the three factors based on metabolome. The Cd stress and reduced N supply also affected leaf metabolites of tomato plants, even though the effects on metabolites and physiology were less than that of elevated temperature. Trend analysis of the metabolites showed eight types in tomatoes under eight treatments. Biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, linoleic acid metabolism and ABC transporters pathways positively responded to the elevated temperature. Moreover, there were significant interactions between the three factors (temperature, CdCl₂ and N) on tomato physiological and morphological parameters. We concluded that the physiological and metabolic responses of tomato plants were more pronounced to the elevated temperatures as compared with cadmium stress and reduced nitrogen fertilizer. This study can support the understanding of complex regulatory mechanisms in plants responding to multiple environmental changes due to climate change, management practice and environmental pollution.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109498"},"PeriodicalIF":6.1,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing plant growth-promoting bacteria (Herbaspirillum seropedicae) from an optimal mineral nitrogen supply: A study on improving nitrogen use efficiency in marandu palisadegrass.","authors":"Cássio Carlette Thiengo, Fernando Shintate Galindo, Bruna Wurr Rodak, João Victor Silva Bernardes, Letícia Oliveira da Rocha, Salete Aparecida Gaziola, Ricardo Antunes Azevedo, Diego Lang Burak, Fábio Lopes Olivares, José Lavres","doi":"10.1016/j.plaphy.2025.109497","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109497","url":null,"abstract":"<p><p>Increasing nitrogen use efficiency (NUE) remains a crucial topic in contemporary agriculture. Inoculation with endophytic diazotrophic bacteria offers a potential solution, but the results vary with the N-fertilization regime. Here, we examined the efficacy of inoculation with Herbaspirillum seropedicae strain HRC54 in enhancing NUE and promoting the growth of Marandu palisadegrass with varying levels of N-urea (0, 25, 50, and 100 mg N kg soil⁻¹). We evaluated NUE indicators and conducted complementary analyses covering biochemical, physiological, nutritional and growth-related parameters after cultivating the plants within a greenhouse environment and maintaining controlled conditions of temperature and humidity. Growth promotion was evident in inoculated plants receiving up to 50 mg N kg soil^-1, with enhanced root growth orchestrating the improvement in NUE. Inoculation also improved the nutritional status of the plants (increased N and P accumulation and N balance index) and increased photosynthesis-related parameters, resulting in increased biomass yield. Insufficient N supply led to oxidative stress (overproduction of H₂O₂ and MDA), which was associated with a reduction in photosystem II efficiency, chlorophyll concentration, and soluble proteins, but only in plants that did not receive microbial inoculation. Conversely, a high N supply (100 mg N kg soil^-1) combined with H. seropedicae had no synergistic effect, as NUE and the associated benefits did not improve. Therefore, inoculation with H. seropedicae is effective at increasing NUE when combined with moderate N rates. These findings support a more rational use of N fertilizers to optimize inoculation benefits and enhance NUE in tropical forage crops.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109497"},"PeriodicalIF":6.1,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tomato biodiversity reveals landrace enhanced drought-adaptive strategy.","authors":"A Pompeiano, T M Moles, L Mariotti, A Santaniello, D Di Baccio, A Scartazza, T Huarancca Reyes, L Guglielminetti","doi":"10.1016/j.plaphy.2025.109495","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109495","url":null,"abstract":"<p><p>Tomato (Solanum lycopersicum L.) is a major crop in the Mediterranean basin, vulnerable to drought at any crop stage. Landraces are traditional, locally adapted varieties with greater resilience to water scarcity than modern cultivars. This study compares the responses of Ciettaicale (CE), a tomato landrace, with Moneymaker (MM), a commercial variety, to controlled soil water deficit at early vegetative stage using biometric, physiological, biochemical, and molecular analyses. Our data highlighted that CE copes better with prolonged and severe drought stress, activating distinct response mechanism. CE sustained higher root water content, and root-to-shoot biomass ratio under drought compared to MM, which may be related to their phytohormones balance. Although pigment responses to drought did not differ markedly, the main ratios revealed different defense mechanisms. Both genotypes showed opposite trends in non-photochemical quenching (NPQ) and actual photon yield of PSII photochemistry under drought stress, with increasing NPQ while decreasing PSII electron transport rate and CO₂ uptake capacity. However, differences in substomatal CO₂ concentration indicated that drought mainly limits photosynthesis through diffusive resistances in CE and metabolic impairment in MM. Changes in antioxidant redox status and activities highlighted the CE ability to activate cellular processes to partially control oxidative stress and to induce a drought acclimation. Multicanonical analysis revealed clear genotype separation along the drought gradient, except for CE, which showed complex drought response and introgression of tolerance traits, particularly under moderate stress. Utilizing such genotypes can significantly improve horticultural production under drought conditions.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109495"},"PeriodicalIF":6.1,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of AtDPBF3, encoding a key member of the ABI5 subfamily involved in ABA signaling, in Arabidopsis thaliana under salt stress.","authors":"Linjie Sun, Xiangkai You, Li Gao, Wuwu Wen, Yuncheng Song, Zhiyu Shen, Qiang Xing, Yuan An, Peng Zhou","doi":"10.1016/j.plaphy.2025.109494","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109494","url":null,"abstract":"<p><p>Soil salinization is a major environmental stress limiting plant growth and development, affecting crop yields worldwide. We investigated the role of AtDPBF3, encoding a key member of the ABI5 subfamily, in the response to salt stress. The AtDPBF3 mutant (dpbf3) was significantly more sensitive to salt stress compared with wild type. Compared with leaves of salt-stressed wild type, those of salt-stressed dpbf3 exhibited severe decreases in chlorophyll content and photochemical efficiency (Fv/Fm), and disrupted ion homeostasis (higher Na⁺ content and lower K⁺ content). Comparative transcriptome analyses identified 457 genes that were differentially expressed in wild-type plants under salt stress but not in dpbf3 under salt stress. These differentially expressed genes encoded a range of products, including ion channels (e.g., AtCXX5, encoding a high-affinity K⁺ uptake/Na⁺ transporter), regulatory protein [e.g., AtSOS3, encoding Salt Overly Sensitive 3 (SOS3) that regulates SOS1 to reduce cytoplasmic Na⁺ levels through the SOS signaling pathway], sugar transporters [e.g., AtSUT4, encoding sucrose transporter 4 (SUT4)], and proteins involved in the stress response (e.g., AtLEA4-5, encoding LEA family proteins) and hormone signaling. These findings suggest that AtDPBF3 enhances salt tolerance by regulating many genes. qRT-PCR analyses confirmed the reliability of the transcriptome data, supporting the crucial role of AtDPBF3 in the salt stress response. These results lay the foundation for further research on the ABA signaling pathway and stress resistance mechanisms.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109494"},"PeriodicalIF":6.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in growth, ionomic and antioxidative enzymes system responded to neutral and alkali salt exposure in halophyte Haloxylon ammodendron seedlings.","authors":"Yan Lu, Fanjiang Zeng, Zhihao Zhang, Ping Lv, Bin Liang","doi":"10.1016/j.plaphy.2025.109492","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109492","url":null,"abstract":"<p><p>Soil salinity and alkalinity severely suppress plant growth and crop yields. This study compared the effects of neutral and alkaline salt exposure, both individually and mixed, on metal content and morphophysiological responses in halophyte Haloxylon ammodendron. Our results showed that alkaline salt exposure more considerably inhibited the growth and photosynthesis of H. ammodendron than neutral salt exposure. Under neutral salt conditions, Na accumulated significantly, while K and Fe absorption was hindered. In contrast, under alkaline salt stress, Na accumulation was more pronounced, leading to a greater inhibition of K absorption. Additionally, Ca accumulation was promoted, while the transport of Fe, Mg, and Cu from root to shoot was suppressed. Alkaline salt stress also induced more severe osmotic stress, triggering a stronger accumulation of soluble sugars to counteract it. Furthermore, seedlings under alkaline stress showed higher levels of REL, H₂O₂, and MDA, but lower activities of SOD, POD, CAT, and APX, indicating increased oxidative damage. These findings suggest that H. ammodendron can adapt well to neutral salt stress through efficient antioxidant enzyme systems and osmotic stress regulation. In contrast, alkaline stress severely inhibits the absorption and transport of mineral elements and disrupts the balance of antioxidant enzymes. Besides, the deleterious effects of neutral-alkaline salt mixed stress were significantly less than those of alkaline stress alone, indicating a reciprocal enhancement between neutral and alkaline salt stress was occurred.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109492"},"PeriodicalIF":6.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MtCIR2 negatively regulates seed germination to salt stress by disrupting metabolisms and signaling of abscisic acid and gibberellins.","authors":"Xiaohan Sun, Rui Tian, Mingui Zhao, Jijun Yan, Jinfang Chu, Wen-Hao Zhang","doi":"10.1016/j.plaphy.2025.109493","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109493","url":null,"abstract":"<p><p>Emerging evidence indicates that long non-coding RNAs (lncRNAs) play a regulatory role in plant response to environmental stresses. Seed germination is a complex physiological process modulated by many environmental and phytohormonal cues. However, how lncRNAs and phytohormones interactively regulate the response of seed germination to salt stress remain largely unknown. Here, we functionally characterized a salt-responsive lncRNA from legume species Medicago truncatula, referred to as MtCIR2, in response to salt stress during seed germination by heterologously expressing MtCIR2 in Arabidopsis in which none such homologous sequence was detected. Expressing MtCIR2 in Arabidopsis rendered the seed germination more sensitive to salt stress. We further evaluated whether and how abscisic acid (ABA) and gibberellin (GA) were involved in the MtCIR2-mediated seed germination in response to salt stress. We found that expression of MtCIR2 led to an increase in endogenous ABA concentration and a decrease in overall GA concentration due to enhanced expression of ABA catabolic gene CYP707A2 and suppressed expression of the genes of GA20ox1, GA20ox2, and GA20ox5 involved in GA synthesis under salt stress, respectively. The MtCIR2-dependent enhanced endogenous ABA and reduced endogenous GA concentrations in seeds resulted in greater suppression of seed germination in transgenic seeds than in wild-type seeds when exposed to salt stress. These findings highlight a regulatory role of lncRNAs in response to salt stress during seed germination.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109493"},"PeriodicalIF":6.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coding and non-coding transcripts modulated by transparent and blue PET micro-nanoplastics in Arabidopsis thaliana.","authors":"Marco Dainelli, Ilaria Colzi, Domenico Giosa, Gaetano Gargiulo, Carla Lo Passo, Ida Pernice, Sara Falsini, Sandra Ristori, Sara Pignattelli, Alice Miniati, Pietro Morandi, Matteo Buti, Chiara Vergata, Andrea Coppi, Cristina Gonnelli, Federico Martinelli","doi":"10.1016/j.plaphy.2024.109409","DOIUrl":"https://doi.org/10.1016/j.plaphy.2024.109409","url":null,"abstract":"<p><p>To get further insights on the micro-nanoplastic (MNP) effects on plants, the aim of this study was to evaluate the response of hydroponically cultivated Arabidopsis thaliana to the presence of differentially colored polyethylene terephthalate (PET) particles. MNP impacts on the root organ were studied at a molecular level, with a special focus on the role of long non-coding RNAs (lncRNAS) in the regulation of gene expression after PET exposure. MNPs of transparent (Tr-PET) and blue (Bl-PET) material at environmentally realistic concentration caused a significant reduction in root length, while only Bl-PET significantly reduced rosette area. MNPs induced oxidative stress markers. Tr-PET upregulated genes involved in signaling of xenobiotics, whereas Bl-PET scarcely affected root transcriptomic profile, activating few gene categories for abiotic stresses. Regarding hormones, genes involved in ABA response were repressed, while brassinosteroid-related genes were differentially regulated by Tr-PET. Both MNPs, but especially Tr-PET, upregulated major latex protein-related genes. Plant molecular response to MNPs was linked to differential abundance of lncRNAs on both comparisons. Tr-PET affected the expression of much more lncRNAs than bl-PET (80 and 11 respectively). These lncRNAs were predicted to interact with several repressed protein-coding genes (i.e. glucosyltransferase UGT2, oxidative stress genes etc.), with possible effects on their regulation. A lncRNA (AT1G09297) interacted with CYP81D8, a key gene of cytochrome P450 gene family involved in xenobiotics detoxification. Two lncRNAs interacted with two members of repressed HSP (HSP90 and HSP17.4) family. Finally, genes involved in redox detoxification and stress responses were inhibited by the interaction with two microplastics-regulated lncRNAs. These data highlighted the need of investigating non-coding RNAs in the future in addition to the mostly studied protein coding transcriptome.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109409"},"PeriodicalIF":6.1,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulling nutrients from Mo-polluted soil by arbuscular mycorrhizal fungi extraradical mycelia is quenching thirsty with poison.","authors":"Mengge Zhang, Zhaoyong Shi, Jiakai Gao, Jiayi Yan, Shouxia Xu, Shuangshuang Wang","doi":"10.1016/j.plaphy.2025.109488","DOIUrl":"https://doi.org/10.1016/j.plaphy.2025.109488","url":null,"abstract":"<p><p>As an extension of plant root system, arbuscular mycorrhizal fungi (AMF) extraradical mycelium (ERM) can break the limitation of rhizosphere and play an important role in plant nutrient acquisition. However, it remains unclear whether ERM is smart enough to pick out nutrients while avoiding poison, or is unable to pick out nutrients and have to absorb poisons together. Therefore, the present study employed a compartment device to separate the mycelia from roots, aiming to explore the nutrient absorption pathways of mycelia in molybdenum (Mo) pollution soil after inoculation with AMF in maize and vetch plants. The results showed that mycelia increased the absorption of nutrients in maize and vetch plants, while reducing the biomass of the plants. In addition, mycelia increased the Mo concentration of plants. The highest contribution of mycelia to Mo concentration in plants reached 114.06%. For different plants, maize tends to immobilize Mo in roots, while vetch tend to transport Mo to shoots under Mo stress. Additionally, variance partitioning analysis proved that the contribution of nutrients to biomass was the largest, with the interpretation rates reaching 65.09% and 47.26% in maize and vetch plants, respectively. The decrease in plant biomass was due to the toxic effects of Mo. Furthermore, the negative effect of the interaction of Mo, mycelia and nutrients on biomass further proved that the nutrients absorption of mycelia under Mo-polluted soil may be carried out in the way of quenching thirsty with poison.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109488"},"PeriodicalIF":6.1,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}