Plant Physiology and Biochemistry最新文献

{"title":"GIGANTEA functions as a co-repressor of cold stress response with a histone-modifying complex","authors":"Myung Geun Ji ,&nbsp;Jin-Sung Huh ,&nbsp;Chae Jin Lim ,&nbsp;Gyeongik Ahn ,&nbsp;Joon-Yung Cha ,&nbsp;Song Yi Jeong ,&nbsp;Gyeong-Im Shin ,&nbsp;Aliya Alimzhan ,&nbsp;Dae-Jin Yun ,&nbsp;Woe-Yeon Kim","doi":"10.1016/j.plaphy.2025.109801","DOIUrl":"10.1016/j.plaphy.2025.109801","url":null,"abstract":"<div><div>The circadian clock in plants is crucial for regulating stress responses, including cold tolerance. Cold stress induces the expression of <em>C-REPEAT BINDING FACTOR</em> (<em>CBF</em>) transcription factors, which activate <em>COLD-REGULATED</em> (<em>COR</em>) genes to mitigate cold-induced damage. Previously, we identified that HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE15 (HOS15)-HISTONE DEACETYLASE 2C (HD2C) complex regulates cold tolerance by modulating histone deacetylation on the <em>COR</em> genes. Our research reports that the circadian oscillator GIGANTEA (GI) regulates the association of histone deacetylase complex on the <em>COR</em> promoter, controlling cold tolerance. We show that GI functions downstream of HOS15, as the <em>hos15-2 gi-2</em> double mutant exhibits freezing tolerance and expression of the <em>COR</em> gene like <em>gi-2</em>. Consistent with the HOS15, GI doesn't affect <em>CBF</em> transcription, suggesting that GI involved cold stress responses through HOS15-mediated <em>COR</em> gene regulation. Moreover, GI reduces histone acetylation and CBF binding at the <em>COR15A</em> promoter under cold stress, repressing <em>COR15A</em> gene expression. We further demonstrate that GI forms a co-repressor with HOS15 and HD2C, inhibiting CBF binding and preventing <em>COR</em> gene activation under normal conditions. These findings provide insights into molecular mechanisms by which GI, HOS15, and HD2C coordinate cold stress responses, offering potential strategies for enhancing plant cold tolerance through chromatin-mediated regulation.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109801"},"PeriodicalIF":6.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thirsty for solutions: How potassium drives sugarcane's varietal-specific strategies for drought tolerance","authors":"José Lavres ,&nbsp;Fernanda Viginotti Alves ,&nbsp;Nikolas Souza Mateus ,&nbsp;Jessica Bezerra Oliveira ,&nbsp;Laura Panzarin Nerastri ,&nbsp;Salete Aparecida Gaziola ,&nbsp;Paulo Mazzafera ,&nbsp;Ricardo Antunes Azevedo ,&nbsp;Lionel Jordan-Meille","doi":"10.1016/j.plaphy.2025.109791","DOIUrl":"10.1016/j.plaphy.2025.109791","url":null,"abstract":"<div><div>With increasing drought stress impacting plants during critical growth stages, genetic breeding and nutrient management are key strategies for plant's resilience. To investigate how potassium (K) fertilization and drought stress impact biochemical, physiological and nutritional mechanisms of sugarcane, we applied a multitiered approach at a range of leaf- and plant-levels in four sugarcane varieties (IACSP95-5000, CTC7, CTC14, and RB975201) grown under two K-soil availability levels (moderate – MK; high K – HK) and two water regimes (well-watered – WW; drought-stressed – DS). DS reduced leaf water potential at predawn (Ψpd) and midday (Ψmd), and photosynthetic parameters, including CO₂ assimilation (<em>A</em>), stomatal conductance (<em>gs</em>), transpiration (<em>E</em>), instantaneous carboxylation efficiency (<em>k</em>), and Fv/Fm. In contrast, DS enhanced K-uptake efficiency (KUpE), oxidative stress (indicated by H₂O₂ and MDA), and enzymatic antioxidant activities (SOD, CAT, APX, GPX). Regarding K-soil availability levels, the HK increased K-uptake efficiency (KUpE) and K-accumulation, which enhanced photosynthetic parameters, enzymatic antioxidant activities and biomass production, while reduced leaf δ¹³C and oxidative stress relative to MK, regardless the water regimes. Plants under DS x HK showed distinct leaf metabolic profiles, being characterized by the accumulation of stress-resilient metabolites, including organic acids (aconitic, aspartic, benzoic glutonic malic and valeric acid), sugars (ribulose, hexopyranose and turanosis), amino acids (alanine), and polyamines (putrescine), notably in CTC7 variety. Our findings illustrate that enhanced K-accumulation observed in plants under HK (especially in CTC7 variety) supports drought resilience across varieties by upregulating biochemical, physiological and nutritional mechanisms.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109791"},"PeriodicalIF":6.1,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143682543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ectopic expression of Dryopteris fragrans DfMTPSL6, a directly target gene of DfWRKY16/45, enhanced drought tolerance in tobacco plants","authors":"Dongrui Zhang,&nbsp;Xun Tang,&nbsp;Xiaojie Qiu,&nbsp;Jiameng Su,&nbsp;Qian Ma,&nbsp;Yongjia Li,&nbsp;Ying Chang","doi":"10.1016/j.plaphy.2025.109786","DOIUrl":"10.1016/j.plaphy.2025.109786","url":null,"abstract":"<div><div>Terpenoid synthesis in seed plants is primarily catalyzed by Typical Terpene Synthase (TPS) enzymes. However, terrestrial non-seed plants also possess microbial terpene synthase-like (MTPSL) enzymes for terpene synthesis. A previous study has demonstrated the presence of both TPSs and MTPSLs in <em>Dryopteris fragrans</em> (L.) Schott. Specifically, DfMTPSL6 has been identified as the enzyme responsible for catalyzing the conversion of farnesyl diphosphate (FPP) to nerolidol. Nerolidol has several functions, including insect, disease and chilling resistance, although its biological role in eukaryotes remains to be confirmed. Transcription factors regulate the terpenoid biosynthesis by binding to gene promoters. However, the regulation of terpene metabolism by transcription factors, including <em>MTPSLs</em>, has not been investigated in ferns.</div><div>This study analyzed the conservation of DfMTPSL6, a nerolidol synthase, expressed in tobacco plants to enhance drought tolerance. Promoter analysis revealed specific expression in glandular hairs, with the active site responsive to MeJA and PEG treatments and containing a W-box, a binding site for WRKY transcription factors. 48 DfWRKY transcription factors were identified, and their expression patterns under MeJA and PEG treatments were analyzed. Yeast one-hybrid assays identified DfWRKY16 and DfWRKY45 as potential regulators of <em>DfMTPSL6</em>. Subcellular localization and transcriptional activation analysis confirmed that DfWRKY16 and DfWRKY45 are transcriptional activator and promotion of <em>DfMTPSL6</em> expression.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109786"},"PeriodicalIF":6.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-day shading increases soluble sugar content in citrus fruit primarily through promoting sucrose distribution, starch degradation and sucrose storage ability","authors":"Fatima Zaman ,&nbsp;Dong-Hai Liu ,&nbsp;Yong-Zhong Liu ,&nbsp;Muhammad Abbas Khan ,&nbsp;Shariq Mahmood Alam ,&nbsp;Yin Luo ,&nbsp;Han Han ,&nbsp;Yan-Ting Li ,&nbsp;Abdelmonem Elshahat","doi":"10.1016/j.plaphy.2025.109779","DOIUrl":"10.1016/j.plaphy.2025.109779","url":null,"abstract":"<div><div>Sugar is a key factor, significantly affecting fruit flavor quality through sucrose metabolism and accumulation. This study investigated the effect of short-day shading on the accumulation of soluble sugars in citrus fruits by using ‘Nanfeng’ tangerine (<em>Citrus reticulata</em> cv. Nanfeng) as materials. Results revealed that using a black net with 12 % light transmittance for 18-day shading remarkably increased soluble sugars and decreased starch content in fruits. Moreover, expresion levels of two cell wall invertase genes (<em>CwINV6</em>/<em>7</em>) and one sucrose synthase genes (<em>CsSUS6</em>) in the segment membrane (SM) were increased significantly at 18 DAS. On the other hand, in juice sacs (JS), expression levels of vacuolar invertase (<em>VINV)</em>, five sucrose synthase genes (<em>CsSUS1</em>-<em>5</em>), two sucrose phosphate synthases (<em>CsSPS1</em>/<em>3</em>), some sugar transporter genes including <em>STP7</em>, <em>SUT1</em>/<em>2</em>/<em>3</em>, two SWEET genes (<em>SWEET5</em>/<em>10</em>), two vacuolar glucose transporter genes (<em>VGT1</em>/<em>2</em>), two tonoplast monosaccharide transporter genes (<em>TMT1/2</em>), and two citrus type I V-PPase genes (<em>CsVPP1</em>/<em>2</em>) were increased significantly at 18DAS as compared to control. Furthermore, starch degradation related gene <em>ISA3, BAM3, GWD and PWD</em> were also significantly induced by 18-day shading. Overall, this study suggested that short-day shading (12 %) enhances soluble sugar accumulation in citrus fruit, primarily by promoting starch degradation and soluble sugar storage in the JS, except for the increase of sucrose distribution to the fruits.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109779"},"PeriodicalIF":6.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143682549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of beneficial microorganisms and phenolic compounds in hydroponically cultivated tomato plants","authors":"María Hurtado-Navarro ,&nbsp;Paula Garcia-Ibañez ,&nbsp;Jose Antonio Pascual ,&nbsp;Micaela Carvajal","doi":"10.1016/j.plaphy.2025.109793","DOIUrl":"10.1016/j.plaphy.2025.109793","url":null,"abstract":"<div><div>The combined effects of applying extracts derived from plant debris and biostimulant microorganisms have not been thoroughly investigated. Furthermore, the interaction between these components and plants remains poorly understood. Utilizing the commercial cherry tomato (<em>Solanum lycopersicum</em> cv. Unidarkwin) as a study model, we conducted a hydroponic experiment in a controlled growth chamber to assess the impact of foliar application of phenolic compounds extracted from <em>Vitis vinifera</em> leaves, combined with the inoculation of <em>Trichoderma harzianum</em> or <em>Bacillus velezensis</em> via roots. Plant growth, gas exchange and root architecture were measured and mineral nutrients, chlorophylls and phenolic compounds were analysed. The results showed that phenolic compounds produced an increase in root fresh weight, by the enhanced root length. This could be related to the improved transpiration rate, sub-stomatal CO₂ concentration, phosphorus and iron concentration in the roots. A positive effect was also found by <em>B. velezensis</em> application in root length development that could be related to the increase in hydraulic conductance. However, <em>T. harzianum</em> inoculation only showed higher root diameter and volume in combination to phenolic application, but with no effect on growth. The absence of caffeic acid and sinapic acid in the Hoagland solution used in the <em>B. velezensis</em> treatments and of chlorogenic acid in all treatments with added microorganisms suggested their metabolization. Therefore, our findings establish that the phenolic exudation could regulate the interaction of microorganism with plants resulting in beneficial physiological changes.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109793"},"PeriodicalIF":6.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of stay-green candidate gene TaTRNH1-3B and development of molecular markers related to chlorophyll content and yield in wheat (Triticum aestivum L.)","authors":"Xiu-Juan Jin ,&nbsp;Xue Yan ,&nbsp;Feng Guo ,&nbsp;Ling Wang ,&nbsp;Juan Lu ,&nbsp;Xiao-Sha Tang ,&nbsp;Hui-Fang Hao ,&nbsp;Md Ashraful Islam ,&nbsp;Ning Li ,&nbsp;Jin-Wen Yang ,&nbsp;Ya-Ping Cao ,&nbsp;Rui-Lian Jing ,&nbsp;Dai-Zhen Sun","doi":"10.1016/j.plaphy.2025.109787","DOIUrl":"10.1016/j.plaphy.2025.109787","url":null,"abstract":"<div><div>Functional stay-green characteristic is closely associated with delayed loss in photosynthetic function and increased crop yield. However, the development and application of functional molecular markers based on stay-green-related genes are limited. This study compared and analyzed the differences of SPAD values, photosynthetic parameters, fluorescence parameters, and antioxidant enzyme activities at 0, 10, 18, 22, 26, 30 and 34 days after anthesis, as well as agronomic traits at mature stage between a stay-green line, Tailv113 (TL113), and a non-stay-green cultivar, Jinmai39 (JM39). The results showed that TL113 had higher photosynthetic capacity, photosynthetic efficiency, antioxidant capacity and yield than JM39. Subsequently, a comparative transcriptome analysis was conducted on TL113 and JM39 at 0, 26, and 30 days after anthesis. Analysis showed that senescence-associated co-expressed genes (SCEGs) and stay-green-associated differentially expressed genes (SDEGs) jointly affected wheat leaf senescence, while SDEGs played an important role in the stay green differences between TL113 and JM39. By analyzing the SNP sites of the SDEGs from transcriptome sequencing, a nsSNP was found in the <em>TaTRNH1-3B</em> sequence between TL113 and JM39. Further analyzing the resequencing data published in the Wheat Union database, four linked SNP sites were identified in <em>TaTRNH1-3B</em>, which formed two haplotypes, <em>TaTRNH1-3B-Hap1</em> and <em>TaTRNH1-3B-Hap2</em>. Based on the SNP at 373 bp (A/G), a CAPS molecular marker, <em>TaTRNH1-3B-Nla</em> III<em>-CAPS,</em> was developed to distinguish allelic variations (A/G). Association analysis between <em>TaTRNH1-3B</em> allelic variation and agronomic traits found that the accessions possessing <em>TaTRNH1-3B-Hap1</em> (A) exhibited significantly higher SPAD values than those possessing <em>TaTRNH1-3B-Hap2</em> (G) in 6 of 10 environments at the jointing stage and in 7 of 10 environments at the grain filling stage in Beijing. Similarly, the accessions possessing <em>TaTRNH1-3B-Hap1</em> (A) exhibited significantly higher chlorophyll contents and yield than those possessing <em>TaTRNH1-3B-Hap2</em> (G) in 3 environments in Taigu. Additionally, lines with <em>TaTRNH1-3B-Hap1</em> (A) displayed higher SPAD values at 0, 15, and 20 days after anthesis in the two BC3F3 populations than those with <em>TaTRNH1-3B-Hap2</em> (G). These results suggest that <em>TaTRNH1-3B</em> is associated with the stay-green and yield traits in wheat, and <em>TaTRNH1-3B-Hap1</em> is a favorable stay-green haplotype. The newly developed molecular marker, <em>TaTRNH1-3B-Nla</em> III<em>-CAPS</em>, provide valuable information for wheat genetic improvement of stay-green and high-yield traits, and can be used to marker-assisted selection breeding.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109787"},"PeriodicalIF":6.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CtMYB63 enhances the waterlogging tolerance of safflower through the JA signalling pathway","authors":"Yingqi Hong ,&nbsp;Shiwen Zhou ,&nbsp;Jianyi Zhang ,&nbsp;Yanxi Lv ,&nbsp;Na Yao ,&nbsp;Xiuming Liu","doi":"10.1016/j.plaphy.2025.109774","DOIUrl":"10.1016/j.plaphy.2025.109774","url":null,"abstract":"<div><div>MeJA can help plants resist external stress, and waterlogging stress is the most serious stress for safflower. The mechanism by which MeJA (Methyl jasmonate) induction helps safflower resist waterlogging stress is unclear. Our results indicate that <em>CtMYB63</em> responds to MeJA through the TGACG motif element, and MeJA induction can further increase the expression of <em>CtMYB63</em>. Under MeJA induction, <em>CtMYB63</em> is expressed by regulating the transcriptional expression of <em>CtDFR1</em>, <em>CtANS1</em> and <em>CtANR1</em>, thereby increasing the biomass and flavonoid content of safflower, but inhibiting plant elongation. Our waterlogging stress experiments further demonstrated that overexpression of <em>CtMYB63</em> can enhance antioxidant enzyme activity to clear the accumulation of MDA (Malondialdehyde), H₂O₂, and O₂⁻. We found that MeJA induction could further improve the waterlogging stress tolerance of overexpressed <em>CtMYB63</em> and WT (wild-type) safflower. Still, the waterlogging tolerance of <em>CtMYB63Δ</em> was weakened due to the deletion of the TGACG motif element. Finally, we found through yeast one-hybrid (Y1H) and luciferase assays that <em>CtMYB63</em> regulates the expression of downstream genes by binding to the promoters of downstream genes. However, <em>CtJAZ9</em> inhibits the expression of downstream genes. In summary, our experiments show that <em>CtMYB63</em> enhances the waterlogging tolerance of safflower through the JA signalling pathway, providing a new idea for improving safflower yield through molecular breeding.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109774"},"PeriodicalIF":6.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the Helicase activity of Pif-like Helicases from Arabidopsis thaliana and role in gene regulation and abiotic stress response","authors":"Himanshi Sharma,&nbsp;Rohini Garg","doi":"10.1016/j.plaphy.2025.109771","DOIUrl":"10.1016/j.plaphy.2025.109771","url":null,"abstract":"<div><div>Regulation in gene expression is rendered by the dynamic structural transitions between classical B-form DNA and non-canonical DNA folds such as G-Quadruplexes (GQS). Several proteins interact with GQSes and regulate a range of biological processes including regulation of DNA replication, transcription, and translation. Several GQS helicases resolve these structures and contribute to maintaining genomic stability. Arabidopsis has 3 homologs of Pif-Like-Helicases (AtPLHs) which have been characterized in this study. Cellular localization and tissue-specific profiling of these homologs revealed nuclear localization and expression in seedling, leaves, and flower tissues suggesting they might play a role in gene regulation and plant development. Molecular characterization of recombinant AtPLH2 and AtPLH3 suggested DNA binding, ATPase, and helicase activity of these two proteins. Further, phenotyping of <em>Atplh</em> mutants revealed alterated seedling root and hypocotyl growth and pollen germination. Transcriptomic profiling of mutants revealed the upregulation of genes associated with processes like systemic acquired resistance, glutathione metabolic process, cellular oxidant detoxification, β-glucosidase activity, SAM-dependent methyltransferase activity, heme binding, calmodulin binding, etc, with these genes harboring WRKY and AP2/EREB TF binding sites in their promoters. These mutants showed susceptibility to different abiotic stresses like salinity and ABA stress as well as to hydroxyurea. Further, <em>AtPLH2</em> and <em>AtPLH3</em> mutants showed no root elongation under low pH suggesting root elongation is affected in the absence of these genes. Our study highlights the role of these GQS helicases in regulating genes involved in root development and stress tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109771"},"PeriodicalIF":6.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deletion of the PtrDJ1C gene leads to increased branching in poplar","authors":"Jingxia Du ,&nbsp;Chunxue Shao ,&nbsp;Dong Wang ,&nbsp;Zizhuo Feng ,&nbsp;Chuwen Cui ,&nbsp;Ruili Li ,&nbsp;Pawan Kumar Jewaria ,&nbsp;Xuanyi Wang ,&nbsp;Jianwei Xiao ,&nbsp;Xinwei Wang","doi":"10.1016/j.plaphy.2025.109789","DOIUrl":"10.1016/j.plaphy.2025.109789","url":null,"abstract":"<div><div>The <em>PtrDJ1C</em> gene is essential for poplar growth and early chloroplast development. Disruption of <em>PtrDJ1C</em> expression results in an albino leaf phenotype and increased branching. However, the underlying mechanism for the increased branching remains unknown. In this study, we employed integrated approaches to investigate the function of <em>PtrDJ1C</em> in the branch-increasing phenotype. Our results revealed that levels of indole-3-acetic acid (IAA), gibberellin (GA), and abscisic acid (ABA) were significantly reduced in <em>ptrdj1c</em> mutants, while cytokinin (CK) levels were slightly increased. Transcriptomic and proteomic analyses identified several key genes and proteins involved in hormone regulation and branching development that were differentially expressed. Specifically, the expression levels of <em>TAA</em>, <em>ZEP</em>, and <em>GA20ox—</em>genes involved in IAA, GA, and ABA biosynthesis—were significantly reduced in <em>ptrdj1c</em>, while <em>IPT</em> and <em>LOG</em>, which regulate CK synthesis, were upregulated. Moreover, immunoblot analysis further validated reduced levels of key biosynthetic enzymes for IAA, GA, and ABA, alongside increased levels of IPT and LOG enzymes. Interestingly, our findings suggest that hormone signaling pathways act in concert with the transcription factor WUSCHEL (WUS) to synergistically promote branching development. These results provide novel insight into the regulatory role of <em>PtrDJ1C</em> in hormone balance and its downstream effects on poplar branching.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109789"},"PeriodicalIF":6.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics change the safe production ability of arsenic-stressed rice (Oryza sativa L.) by regulating the antioxidant capacity, arsenic absorption, and distribution in rice","authors":"Yueyi Wu ,&nbsp;Chaorui Yan ,&nbsp;Li Yin ,&nbsp;Zhaoyong Zeng ,&nbsp;Deqiang Li ,&nbsp;Yinggang Xu ,&nbsp;Lan Zhang ,&nbsp;Xuesong Gao ,&nbsp;Fu Huang ,&nbsp;Guangdeng Chen","doi":"10.1016/j.plaphy.2025.109792","DOIUrl":"10.1016/j.plaphy.2025.109792","url":null,"abstract":"<div><div>Microplastics (MPs) and arsenic (As) are pervasive pollutants in agricultural soils, drawing increasing attention due to their combined toxicity. While biodegradable plastics offer a potential alternative to conventional plastics, their interactions with As and subsequent effects on edible crops remain largely unexplored. Here, we investigated the combined effect of polyethylene (PE) and polylactic acid (PLA) microplastics with As on rice growth, As accumulation, and rhizosphere microbial communities in two rice genotypes. The results showed that As-PE exposure was more detrimental to rice growth than As alone, leading to biomass reductions of 21.1–39.8% in 2A roots, 32.6–54.6% in stems, and 21.9–32.7% in leaves. In contrast, PLA mitigated As-induced growth inhibition in 2119, increasing leaf biomass by 56.1–71.9% and stem biomass by 45.6–57.9%. The presence of MPs intensified As toxicity and induced oxidative stress, with the low-As-accumulating genotype exhibiting stronger detoxification mechanisms, including enhanced sequestration of As in the leaf cell wall and MPs facilitated As adsorption and desorption in the root zone, exacerbating As accumulation in the aerial part of rice, particularly during grain filling. Different degradation characteristics of MPs altered microbial composition and function, impacting rhizosphere iron plaque formation and As availability in soil. PLA decreased the As content in 2A and 2119 roots by 6.1% and 24.0%, respectively, whereas PE increased by 10.6% and 12.9%. This study provides new insights into the comprehensive toxicity of As and MPs in the soil-plant system, highlighting their effects on As uptake and accumulation in rice.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109792"},"PeriodicalIF":6.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}