Mengyao Wang , Tingting Huang , Zhihong Peng , Shuting Wang , Gangmu Wu , Longwei Xiong , Siren Lan , Donghui Peng , Zhong-jian Liu , Ye Ai
{"title":"An R2R3-MYB transcription factor regulates anthocyanin accumulation in response to temperature signals in Cymbidium ensifolium","authors":"Mengyao Wang , Tingting Huang , Zhihong Peng , Shuting Wang , Gangmu Wu , Longwei Xiong , Siren Lan , Donghui Peng , Zhong-jian Liu , Ye Ai","doi":"10.1016/j.plaphy.2025.110244","DOIUrl":"10.1016/j.plaphy.2025.110244","url":null,"abstract":"<div><div><em>Cymbidium ensifolium</em>, an ornamental plant in China, is prized for its diverse flower colors and high aesthetic value. The flower color of <em>C. ensifolium</em> is significantly affected by temperature. In this research, we examined the impact of temperature on anthocyanin accumulation in the sepals of <em>C. ensifolium</em> (‘Shi Zhang Hong’) through different temperature treatments (T1, 20/15 °C, T2, 28/23 °C, T3, 36/31 °C), combined with metabolomics, transcriptome analysis and various experimental methods, screening for key temperature-responsive genes. The results indicated that high temperatures suppress both red pigmentation and anthocyanin accumulation in sepals, while low temperatures promote this process. Metabolomic analysis indicated that temperature primarily influences sepal coloration by regulating the abundance of cyanidin-3-<em>O</em>-glucoside. Notably, its abundance significantly increases under low temperatures but decreases at high temperatures. <em>CeMYB52</em> was found to be a crucial transcription factor (TF) regulating anthocyanin accumulation by transcriptome analysis, with high expression at low temperatures and low expression at high temperatures. Remarkably, the spatio-temporal expression profiles of <em>CeCHS8</em> and <em>CeGST</em> were aligned with <em>CeMYB52</em>. Through regulating the expression of <em>CeCHS8</em> and <em>CeGST</em>, <em>CeMYB52</em> contributes significantly to the promotion of anthocyanin biosynthesis and transport. Our findings offer a molecular basis for breeding temperature-resilient ornamental plants, contributing to agricultural sustainability under climate change.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110244"},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yanyan Wang , Tongkai Guo , Changyan Tian , Ke Zhang , Zhenyong Zhao , Mingfang Hu , Wenxuan Mai
{"title":"Suaeda salsa adapts to high-salt environments through expanding vessel diameter, activating antioxidant enzymes and strengthening osmotic regulation","authors":"Yanyan Wang , Tongkai Guo , Changyan Tian , Ke Zhang , Zhenyong Zhao , Mingfang Hu , Wenxuan Mai","doi":"10.1016/j.plaphy.2025.110249","DOIUrl":"10.1016/j.plaphy.2025.110249","url":null,"abstract":"<div><div>Halophytes have evolved various mechanisms to adapt to saline conditions. However, their morpho-anatomical changes, along with the root metabolic responses to increased salt stress in saline-alkali soils, remain poorly understood. This study aimed to elucidate the adaptive mechanisms of <em>Suaeda salsa</em> under four saline-alkali soil levels (non-saline; NS, lightly saline; LS, moderately saline; MS, and severely saline; SS) by analyzing their anatomical morphological traits, nutritional components, and root differential metabolites through a combination of imaging and metabolomics technologies. Results showed that increased salt stress markedly enhanced leaf succulence, root vessel diameter, and epidermal thickness. Compared to NS treatment, leaf perimeter increased by 11.48, 28.29 and 47.94 %, respectively; root vessel diameter increased by 11.07, 24.39 and 14.31 % (<em>p</em> < 0.05). Root epidermal thickness increased by 24.34 and 109.37 % in MS and SS treatments, compared to NS treatment (<em>p</em> < 0.05). High salt stress disrupted leaf chloroplast and mitochondrial membranes, as indicated by malondialdehyde (MDA) increases of 32.45, 73.36 and 112.96 % in LS, MS and SS treatments (<em>p</em> < 0.05). Antioxidant enzyme activities (catalase; CAT, peroxidase; POD, superoxide dismutase; SOD) significantly increased in LS, MS, and SS treatments by 49.25–71.89 %, 90.64–278.86 %, and 12.94–116.37 %, respectively (<em>p</em> < 0.05). Elevated soil salinity increased sodium/potassium (Na/K) ratios by 223–518 % in roots, 215–330 % in stems, and 303–524 % in leaves; sodium/calcium (Na/Ca) ratios increased by 99–180 % in roots, 842–1810 % in stems, and 1111–2901 % in leaves. Additionally, root metabolic pathways associated with galactose/alanine, aspartate, and glutamate were markedly enhanced, leading to the upregulation of L-aspartate, β-alanine, glycine, galactinol, glycerol, and galactonic acid. Consequently, halophytes highlight their robust adaptive mechanisms by substantial morphological, physiological, and metabolic adjustments in response to salinity stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110249"},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The relationship between mitochondrial respiration metabolism, energy efficiency and ultraweak luminescence in strawberry fruit","authors":"Li Zhang, Yang Bai, Jinli Guo","doi":"10.1016/j.plaphy.2025.110253","DOIUrl":"10.1016/j.plaphy.2025.110253","url":null,"abstract":"<div><h3>Background</h3><div>Ultraweak luminescence (UWL) is a natural phenomenon characterized by extremely low light emission in living organisms. Although it has attracted scientific attention for decades, its underlying mechanisms remain unclear.</div></div><div><h3>Objective</h3><div>This study aimed to elucidate the relationship between mitochondrial respiratory metabolism and UWL in strawberry fruit mitochondria, focusing on how electron transport and oxidative phosphorylation affect UWL intensity.</div></div><div><h3>Methods</h3><div>Mitochondria from the strawberry variety ‘Hongyan' were treated with activators adenosine diphosphate (ADP) and sodium succinate (C<sub>4</sub>H<sub>4</sub>Na<sub>2</sub>O<sub>4</sub>) or inhibitors 2,4-Dinitrophenol (DNP) and sodium vanadate (NaVO<sub>4</sub>) during ATP synthesis. Comparative analyses assessed mitochondrial respiration, energy parameters, and UWL changes under these treatments.</div></div><div><h3>Results</h3><div>Activator treatments significantly increased respiratory enzyme activities of succinate dehydrogenase (SDH), cytochrome <em>c</em> oxidase (COX) and H<sup>+</sup>-ATPase, respiration rate, respiratory pathway contributions, ATP, ADP, energy charge, and UWL intensity at higher concentrations (<em>P</em> < 0.05). In contrast, inhibitor treatments caused progressive reductions in these indicators and UWL intensity, also reaching significance at higher concentrations. Correlation analysis showed UWL intensity was positively associated with respiration metrics, especially respiration rate and cytochrome pathway contribution (<em>P</em> < 0.05), and strongly correlated with ATP, ADP, and energy charge (<em>P</em> < 0.05 or <em>P</em> < 0.01), while negatively correlated with AMP (<em>P</em> < 0.05). Principal component analysis indicated that energy parameters contributed most to UWL variation, followed by respiration rate and enzyme activities.</div></div><div><h3>Conclusion</h3><div>UWL intensity reflects mitochondrial respiratory metabolism and ATP production, suggesting mitochondria are key organelles in UWL generation. UWL could serve as a potential indicator of mitochondrial function and cellular energy status.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110253"},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hongyi Qin , Jiajing Zeng , Xinrui He , Juan Li , Dalian Lu , Bingfu Lei , Min Zhong , Yunyan Kang , Xirong Chai , Xian Yang
{"title":"Salvia miltiorrhiza-derived carbon dots alleviate cadmium stress in flowering Chinese cabbage by suppressing BrTCP9-mediated cadmium transport and reactive oxygen species metabolism","authors":"Hongyi Qin , Jiajing Zeng , Xinrui He , Juan Li , Dalian Lu , Bingfu Lei , Min Zhong , Yunyan Kang , Xirong Chai , Xian Yang","doi":"10.1016/j.plaphy.2025.110240","DOIUrl":"10.1016/j.plaphy.2025.110240","url":null,"abstract":"<div><div>Cadmium stress not only inhibits vegetable growth but also threatens food safety. Carbon dots (CDs) and TCP transcription factors play crucial roles in responding to abiotic stress. As a leafy vegetable, flowering Chinese cabbage exhibits heightened vulnerability to cadmium contamination. However, the molecular mechanism by which CDs may alleviate cadmium stress through TCP-mediated regulation of cadmium transport and reactive oxygen species (ROS) metabolism remains unclear. In this study, we found that <em>Salvia miltiorrhiza</em>-derived CDs (<em>Sm</em>CDs) alleviated cadmium stress in flowering Chinese cabbage in a concentration-dependent manner. An optimal concentration of <em>Sm</em>CDs significantly enhanced cadmium tolerance and plant growth. Furthermore, TCP transcription factor simultaneously induced by cadmium and suppressed by <em>Sm</em>CDs was identified as BrTCP9, while the cadmium transporter and <em>Rboh</em> gene were identified as <em>BrNramp1.2</em> and <em>BrRbohA</em>, respectively. Under cadmium stress, silencing <em>BrTCP9</em>, <em>BrNramp1.2</em>, or <em>BrRbohA</em> markedly suppressed ROS production, reduced both cadmium and malondialdehyde accumulation, and promoted plant growth, with <em>Sm</em>CDs application further enhancing these effects. Intriguingly, <em>BrNramp1.2</em> and <em>BrRbohA</em> were found to mutually regulate each other under cadmium stress. Y1H, LUC, GUS, and EMSA assays confirmed that BrTCP9 directly binds to the promoter of <em>BrNramp1.2</em> and <em>BrRbohA</em>, activating their expression. These results indicated that BrTCP9, <em>BrNramp1.2</em>, and <em>BrRbohA</em> negatively regulated cadmium tolerance, whereas <em>Sm</em>CDs mitigated cadmium stress by suppressing BrTCP9-mediated <em>BrNramp1.2</em> and <em>BrRbohA</em> expressions. This study not only expanded the molecular network of cadmium stress response but also provided theoretical foundation for the application of carbon nanomaterials in agricultural heavy metal remediation.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110240"},"PeriodicalIF":6.1,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiujun Du , Xinglu Zhou , Hantian Wei , Yongxia Bai , Xueqin Song , Lei Zhang , Jianjun Hu
{"title":"Poplar PdRabG3f inhibits root elongation and increases salt tolerance by enhancing endogenous abscisic acid synthesis","authors":"Jiujun Du , Xinglu Zhou , Hantian Wei , Yongxia Bai , Xueqin Song , Lei Zhang , Jianjun Hu","doi":"10.1016/j.plaphy.2025.110233","DOIUrl":"10.1016/j.plaphy.2025.110233","url":null,"abstract":"<div><div>Proteins belonging to the small GTPases family, particularly Rab proteins, are involved in plant root development. The RabG subfamily proteins, in particular, play a role in regulating plant stress responses. This study revealed that the poplar RabG protein PdRabG3f exhibited tissue-specific activity in root epidermis, root hairs, and vascular stele. Functional characterization further demonstrated that overexpression of <em>PdRabG3f</em> in transgenic poplar significantly inhibited root elongation, reducing maximum root length, and fresh root weight, whereas suppression of <em>PdRabG3f</em> enhanced root growth — a phenotype consistent with its spatial expression pattern. In <em>PdRabG3f</em> overexpression lines, elevated endogenous abscisic acid (ABA) levels were observed, which correlated with enhanced ABA sensitivity and further inhibition of root elongation under exogenous ABA treatment. As a result, these lines exhibited improved resilience under salt stress, including higher peroxidase activity, reduced malondialdehyde content, and maintained relative water content. Transcriptome analysis further supported their enhanced salt tolerance, revealing significant upregulation of stress-responsive genes and enrichment in stress response pathways. These findings establish <em>PdRabG3f</em> as a negative regulator of root growth via ABA biosynthesis and highlight its role in enhancing salt tolerance through ABA-dependent stress signaling. This study provides novel insights into Rab protein-mediated hormonal regulation in woody plants, offering potential targets for improving stress resilience in poplar.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110233"},"PeriodicalIF":6.1,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144632955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mingyang Xu, Xuefeng Zhang, Fan Jiang, He Zhang, Ning Yan, Xinyue Li, Guodong Du
{"title":"The PuWRKY29-PuMYB62 module responds to salicylic acid to inhibit the synthesis of stone cells in ‘Nanguo’ pear","authors":"Mingyang Xu, Xuefeng Zhang, Fan Jiang, He Zhang, Ning Yan, Xinyue Li, Guodong Du","doi":"10.1016/j.plaphy.2025.110238","DOIUrl":"10.1016/j.plaphy.2025.110238","url":null,"abstract":"<div><div>Stone cells serve as pivotal determinants of pear fruit quality, with their formation exhibiting a strong correlation with lignification processes. As a phenolic signaling molecule with multiple biological functions, salicylic acid (SA) plays essential roles in regulating fruit growth and developmental processes. Nevertheless, the molecular mechanisms underlying SA-mediated modulation of stone cell differentiation in pears remain poorly characterized. Our investigation revealed that exogenous application of 15 mg L<sup>−1</sup> SA decreased lignified stone cell formation in ‘Nanguo’ (<em>Pyrus ussuriensis</em>) pear fruits and significantly downregulating the expression of the lignin biosynthesis-related <em>PuPRX64</em>. mRNA-seq identified two transcription factors, PuWRKY29 and PuMYB62, which were significantly induced by SA. Functional characterization through transient expression assays in pear fruits and stable transgenic calli demonstrated that both factors act as negative regulators of stone cell formation and lignin accumulation. PuWRKY29 binds to the W-box <em>cis-</em>element within the <em>PuMYB62</em> promoter to activate its transcription, while simultaneously forming a protein complex that synergistically represses <em>PuPRX64</em> expression. Collectively, our findings suggest that exogenous SA treatment inhibits stone cell and lignin biosynthesis in pears via the PuWRKY29-PuMYB62 regulatory module.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110238"},"PeriodicalIF":6.1,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hui Lyu , Zhaoqi Tang , Weijie Xu , Dejun Kong , Zhihong Wang , Zhixiao Yang , Jishun Zhang , Hongqi Wu , Zili Wang , Dušan Lazár , Yingchao Lin
{"title":"Distinct non-photochemical quenching characteristics under constant and fluctuating light conditions: comparison between local and imported tobacco varieties","authors":"Hui Lyu , Zhaoqi Tang , Weijie Xu , Dejun Kong , Zhihong Wang , Zhixiao Yang , Jishun Zhang , Hongqi Wu , Zili Wang , Dušan Lazár , Yingchao Lin","doi":"10.1016/j.plaphy.2025.110239","DOIUrl":"10.1016/j.plaphy.2025.110239","url":null,"abstract":"<div><div>To explore the variation in adaptability to fluctuating light environments, the non-photochemical quenching (NPQ) characteristics of a local tobacco variety Xiaofeng 8 and a foreign introduced variety K326, under both constant and fluctuating light conditions were examined. The field experiment demonstrated that Xiaofeng 8 exhibited significantly stronger growth compared to K326, with the dry weight per plant and leaf area of Xiaofeng 8 increased by 28.26 % and 32.85 % compared with those of K326, respectively. Further, a distinct activation pattern of NPQ was detected in both varieties during the transition from dark to high light. Building on this finding, our subsequent tests proved that, in the high light intensity segments of a fluctuating light environment, Xiaofeng 8 exhibited a higher NPQ value compared to K326, indicating an enhanced capacity for dissipating excess light energy as thermal energy and mitigating the potential risk of photodamage. Subsequent analysis of the contents of violaxanthin (V), antheraxanthin (A), and zeaxanthin (Z) within the two varieties after high - light treatment has proved that Xiaofeng 8 has a superior ability to dissipate excess absorbed light energy under high - light conditions. This ability may contribute to its better growth under fluctuating light environments. Mutants defective in violaxanthin de-epoxidase (VDE) and zeaxanthin epoxidase (ZEP) were employed to examine their involvement in regulating plants growth under fluctuating light condition. The results showed that defects in these enzymes affected the NPQ response and reduced the growth rate of the mutants under fluctuating light conditions, suggesting its pivotal role of xanthophyll in regulating plant growth and adaptation to variable light environments. Collectively, these findings emphasize the importance of exploring genetic resources improving plant NPQ under fluctuating light conditions from both domestic and imported varieties, thereby providing promising targets for selecting and breeding new crop varieties with improved yield.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110239"},"PeriodicalIF":6.1,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eduardo Carlos Rudell , Luan Cutti , Guilherme Menegol Turra , Paula Sinigaglia Angonese , Vinicius Ferrari Tasca , Othon Dias dos Santos , Eric Patterson , Aldo Merotto
{"title":"Variability and spatial distribution of ALS-inhibitor resistance mechanisms in Brazilian Echinochloa crus-galli","authors":"Eduardo Carlos Rudell , Luan Cutti , Guilherme Menegol Turra , Paula Sinigaglia Angonese , Vinicius Ferrari Tasca , Othon Dias dos Santos , Eric Patterson , Aldo Merotto","doi":"10.1016/j.plaphy.2025.110237","DOIUrl":"10.1016/j.plaphy.2025.110237","url":null,"abstract":"<div><div>Barnyardgrass (<em>Echinochloa crus-galli</em> (L.) P. Beauv.) is a hexaploid weed, commonly found in rice fields. The field-level frequencies of the herbicide resistance mechanisms present in barnyardgrass remain unknown. This study developed and compared molecular marker assays for detecting mutations in the <em>ALS</em> genes, analyzing their frequency and spatial distribution in Southern Brazil rice fields. The <em>ALS</em> gene of 52 accessions was sequenced to identify mutations associated with resistance. Single Nucleotide-Amplified Polymorphism (SNAP) and PCR Allele Competitive Extension (PACE®) markers were developed for detecting ALS mutations: A122T, A205N, W574L, and S653N. A total of 233 accessions that survived imidazolinone application were collected. A greenhouse assay identified 195 and 84 accessions resistant to imazapyr + imazapic and penoxsulam, respectively. Molecular assays detected 188 resistant accessions, with W574L, S653N, A122T, and A205N mutations present in 43 %, 29 %, 17 %, and 5 % of resistant samples, respectively. 6 % of accessions carried mutations in two positions, while six resistant accessions lacked any mutation. Efficiency of SNAP and PACE methods was 90 % and 82 %, respectively. Discrepancies between methods were resolved using Nanopore sequencing. The detection threshold was one resistant per 25 susceptible DNA samples and one per 10 using leaf discs in SNAP. All mutations were distributed geographically, with the frequency of W574L increasing from 40 % in 2017/2018 to 47 % in the 2022/2023 season. ALS resistance was detected in 80 % of the accessions. Epidemiological studies, like this, that track resistance mechanisms, including the occurrence, distribution, and variability of mutations, are crucial for improving weed control recommendations.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110237"},"PeriodicalIF":6.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mengmeng Zhang , Juyong Zhao , Yongbo Yu , Yang Yu , Na Cui , Xiangnan Meng , Haiyan Fan
{"title":"Functional divergence of growth-regulating factors CsGRF1 and CsGRF7 in Cucumis sativus defense against Podosphaera xanthii","authors":"Mengmeng Zhang , Juyong Zhao , Yongbo Yu , Yang Yu , Na Cui , Xiangnan Meng , Haiyan Fan","doi":"10.1016/j.plaphy.2025.110236","DOIUrl":"10.1016/j.plaphy.2025.110236","url":null,"abstract":"<div><div>As plant-specific transcription factors (TFs), growth-regulating factors (GRFs) play pivotal roles in regulating plant growth, development, and stress responses. Previous RNA-seq analysis revealed differential expression of <em>CsGRF1</em> and <em>CsGRF7</em> in cucumber under <em>Podosphaera xanthii</em> stress, suggesting their potential involvement in powdery mildew (PM) resistance. However, the functions and regulatory mechanisms of <em>CsGRF1</em> and <em>CsGRF7</em> in cucumber's defense remain unclear. In this study, we investigated the functional divergence of CsGRF1 and CsGRF7 in cucumber's PM defense. Subcellular localization, transcriptional activity, and protein interaction assays confirmed that both are typical members of the GRF TF family. Functional analyses demonstrated that <em>CsGRF1</em> positively regulates cucumber resistance to <em>P. xanthii</em>, whereas <em>CsGRF7</em> suppresses it. Further studies revealed that <em>CsGRF1</em> enhances disease resistance by maintaining reactive oxygen species (ROS) homeostasis, while CsGRF7 negatively regulates lignin biosynthesis by directly interacting with CsODO1 (an MYB TF that inhibits PM resistance), thereby reducing defense responses. This study elucidates distinct regulatory roles of <em>GRF</em> family members in PM resistance and provides a theoretical foundation for improving cucumber disease resistance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110236"},"PeriodicalIF":6.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Duong Thi Thuy Dang , Linh Thi Nguyen , Anh-Tuan Tran , Tam Thi Thanh Tran , Tuan-Anh Tran , Kieu Thi Xuan Vo , Jong-Seong Jeon , Tien Van Vu , Jae-Yean Kim , Nhue Phuong Nguyen , Ha Hoang Chu , Phat Tien Do , Huong Thi Mai To
{"title":"A novel glycerophosphodiester phosphodiesterase 13 is involved in the phosphate starvation-induced phospholipid degradation in rice","authors":"Duong Thi Thuy Dang , Linh Thi Nguyen , Anh-Tuan Tran , Tam Thi Thanh Tran , Tuan-Anh Tran , Kieu Thi Xuan Vo , Jong-Seong Jeon , Tien Van Vu , Jae-Yean Kim , Nhue Phuong Nguyen , Ha Hoang Chu , Phat Tien Do , Huong Thi Mai To","doi":"10.1016/j.plaphy.2025.110230","DOIUrl":"10.1016/j.plaphy.2025.110230","url":null,"abstract":"<div><div>Inorganic phosphate (Pi) is an essential macronutrient for plant growth and productivity. However, its limited bioavailability in soil poses a major constraint to crop performance. To cope with Pi deficiency, plants have evolved a wide range of physiological and biochemical strategies, including membrane lipid remodeling, to optimize Pi acquisition and internal recycling. In a previous genome-wide association study (GWAS) using Vietnamese rice germplasm, we identified a robust QTL, <em>qRST9.14</em>, associated with Pi efficiency. Notably, <em>OsGDPD13,</em> a gene encoding a glycerophosphodiester phosphodiesterase, is located within this QTL and is absent from the <em>indica</em> reference genome, highlighting potential genetic divergence between rice subspecies. Subcellular localization studies revealed that the OsGDPD13 protein is distributed across multiple cellular compartments, including the plasma membrane, cytoplasmic speckles, and plasmodesmata, suggesting multifunctional roles. To investigate the function of <em>OsGDPD13</em>, knockout mutants and overexpression lines were generated in the <em>japonica</em> cv. Kitaake background. Under Pi-deficient conditions, knockout lines showed impaired phospholipid (PL) degradation, particularly phosphatidylcholine (PC), while overexpression lines exhibited enhanced PC breakdown even under Pi sufficiency. These changes were accompanied by differential expressions of the Pi signaling gene <em>OsSPX1</em>, implicating <em>OsGDPD13</em> in Pi-responsive lipid remodeling and signaling. Promoter analysis further revealed multiple P1BS-like elements, suggesting regulation by MYB-CC transcription factors. These findings suggest <em>OsGDPD13</em> as a key component in the Pi deficiency response and possibly linking membrane lipid remodeling to phosphate homeostasis. This work offers new insights into <em>OsGDPD13</em> function and presents it as a promising genetic target for improving phosphorus use efficiency (PUE) in rice.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110230"},"PeriodicalIF":6.1,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}