CRISPR/ cas9介导的LhNAP突变延长了百合花的寿命

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-09-23 DOI:10.1016/j.plaphy.2025.110551

Kenichi Shibuya , Toshikazu Nomizu , Hayato Morimoto , Kazuhito Satou

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引用次数: 0

摘要

花寿命是决定观赏植物品质的重要性状。NAC / ataf1,2 /CUC2 （NAM/ ataf1,2 /CUC2）转录因子在多种植物叶片和花瓣衰老中起调节作用。为了延长百合的寿命，本研究利用CRISPR/Cas9技术对东方杂交百合“Acapulco”的NAC转录因子编码LhNAP进行了靶向诱变。在玉米泛素启动子驱动下，利用含有靶向LhNAP的一个或两个区域的引导RNA表达盒和经密码子优化的Cas9的二元载体对丝状愈伤组织进行转化。与野生型植物相比，在LhNAP位点携带双等位基因突变的突变系表现出明显延迟的花被片衰老和脱落。总的来说，我们的研究结果突出了LhNAP靶向基因组编辑的潜力，以产生具有延长花寿命的百合，并揭示了LhNAP在调节百合花被片衰老和脱落中的关键作用。

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CRISPR/Cas9-mediated LhNAP mutagenesis extends flower longevity in lily

Flower longevity is an important trait determining the ornamental plant quality. NAM/ATAF1,2/CUC2 (NAC) transcription factors regulate leaf and petal senescence in several plants. To extend the longevity of lily flowers, in this study we used the CRISPR/Cas9 technology for the targeted mutagenesis of LhNAP encoding the NAC transcription factor in the Oriental hybrid lily (Lilium spp.) ‘Acapulco’. Filament-derived calli were transformed with binary vectors containing guide RNA expression cassettes targeting one or two regions of LhNAP and the codon-optimized Cas9 for Oryza sativa driven by Zea mays ubiquitin promoter. Mutant lines harbouring biallelic mutations at the LhNAP locus exhibited clearly delayed tepal senescence and abscission compared to the wild-type plants. Overall, our results highlight the potential of LhNAP-targeted genome editing to produce lilies with extended flower longevity and reveal the crucial role of LhNAP in regulating tepal senescence and abscission in lilies.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.