Plant Physiology and Biochemistry最新文献

{"title":"Ammonium enhances rice resistance to Magnaporthe oryzae through H2O2 accumulation","authors":"","doi":"10.1016/j.plaphy.2024.109058","DOIUrl":"10.1016/j.plaphy.2024.109058","url":null,"abstract":"<div><p>Nitrogen (N) is essential for the physiological processes of plants. However, the specific mechanisms by which different nitrogen forms influence rice blast pathogenesis remain poorly understood. This study used hydroponic assays to explore how ammonium (NH₄⁺) and nitrate (NO₃⁻) affect rice after inoculation with <em>Magnaporthe oryzae</em> (<em>M. oryzae</em>). The results showed that NH₄⁺, compared to NO₃⁻, significantly reduced disease severity, fungal growth, fungal hyphae number, the expansion capacity of infectious hyphae, and disease-related loss of photosynthesis. Additionally, NH₄⁺ enhanced the expression of defense-related genes, including <em>OsPBZ1</em>, <em>OsCHT1</em>, <em>OsPR1a</em>, and <em>OsPR10</em>. NH₄⁺-treated rice also exhibited higher hydrogen peroxide (H₂O₂) accumulation and increased antioxidant enzyme activities. Moreover, susceptibility to rice blast disease increased when H₂O₂ was scavenged, while a reduction in susceptibility was observed with the application of exogenous H₂O₂. These results suggest that ammonium enhances rice resistance to <em>M. oryzae</em>, potentially through H₂O₂ accumulation. The findings provide valuable insights into how different nitrogen forms affect plant immunity in rice, which is crucial for controlling rice blast and ensuring stable food production.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142049809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation in insect herbivory across an urbanization gradient: The role of abiotic factors and leaf secondary metabolites","authors":"","doi":"10.1016/j.plaphy.2024.109056","DOIUrl":"10.1016/j.plaphy.2024.109056","url":null,"abstract":"<div><p>Urbanization impacts plant-herbivore interactions, which are crucial for ecosystem functions such as carbon sequestration and nutrient cycling. While some studies have reported reductions in insect herbivory in urban areas (relative to rural or natural forests), this trend is not consistent and the underlying causes for such variation remain unclear. We conducted a continental-scale study on insect herbivory along urbanization gradients for three European tree species: <em>Quercus robur, Tilia cordata,</em> and <em>Fraxinus excelsior,</em> and further investigated their biotic and abiotic correlates to get at mechanisms. To this end, we quantified insect leaf herbivory and foliar secondary metabolites (phenolics, terpenoids, alkaloids) for 176 trees across eight European cities. Additionally, we collected data on microclimate (air temperature) and soil characteristics (pH, carbon, nutrients) to test for abiotic correlates of urbanization effects directly or indirectly (through changes in plant secondary chemistry) linked to herbivory. Our results showed that urbanization was negatively associated with herbivory for <em>Q. robur</em> and <em>F. excelsior</em>, but not for <em>T. cordata</em>. In addition, urbanization was positively associated with secondary metabolite concentrations, but only for <em>Q. robur</em>. Urbanization was positively associated with air temperature for <em>Q. robur</em> and <em>F. excelsior</em>, and negatively with soil nutrients (magnesium) in the case of <em>F. excelsior</em>, but these abiotic variables were not associated with herbivory. Contrary to expectations, we found no evidence for indirect effects of abiotic factors via plant defences on herbivory for either <em>Q. robur</em> or <em>F. excelsior</em>. Additional biotic or abiotic drivers must therefore be accounted for to explain observed urbanization gradients in herbivory and their interspecific variation.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0981942824007241/pdfft?md5=10a83c92c68377d730858a940501babd&pid=1-s2.0-S0981942824007241-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142058162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strigolactones: A promising tool for nutrient acquisition through arbuscular mycorrhizal fungi symbiosis and abiotic stress tolerance","authors":"","doi":"10.1016/j.plaphy.2024.109057","DOIUrl":"10.1016/j.plaphy.2024.109057","url":null,"abstract":"<div><p>Strigolactones (SLs) constitute essential phytohormones that control pathogen defense, resilience to phosphate deficiency and abiotic stresses. Furthermore, SLs are released into the soil by roots, especially in conditions in which there is inadequate phosphate or nitrogen available. SLs have the aptitude to stimulate the root parasite plants and symbiotic cooperation with arbuscular mycorrhizal (AM) fungi in rhizosphere. The use of mineral resources, especially phosphorus (P), by host plants is accelerated by AMF, which also improves plant growth and resilience to a series of biotic and abiotic stresses. Thus, these SL treatments that promote rhizobial symbiosis are substitutes for artificial fertilizers and other chemicals, supporting ecologically friendly farming practices. Moreover, SLs have become a fascinating target for abiotic stress adaptation in plants, with an array of uses in sustainable agriculture. In this review, the biological activity has been summarized that SLs as a signaling hormone for AMF symbiosis, nutrient acquisition, and abiotic stress tolerance through interaction with other hormones. Furthermore, the processes behind the alterations in the microbial population caused by SL are clarified, emphasizing the interplay with other signaling mechanisms. This review covers the latest developments in SL studies as well as the properties of SLs on microbial populations, plant hormone transductions, interactions and abiotic stress tolerance.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics integrative analysis provided new insights into alkaline stress in alfalfa","authors":"","doi":"10.1016/j.plaphy.2024.109048","DOIUrl":"10.1016/j.plaphy.2024.109048","url":null,"abstract":"<div><p>Saline-alkali stress is one of the main abiotic stresses that limits plant growth. Salt stress has been widely studied, but alkaline salt degradation caused by NaHCO₃ has rarely been investigated. In the present study, the alfalfa cultivar ‘Zhongmu No. 1′ was treated with 50 mM NaHCO₃ (0, 4, 8, 12 and 24 h) to study the resulting enzyme activity and changes in mRNA, miRNA and metabolites in the roots. The results showed that the enzyme activity changed significantly after alkali stress treatment. The genomic analysis revealed 14,970 differentially expressed mRNAs (DEMs), 53 differentially expressed miRNAs (DEMis), and 463 differentially accumulated metabolites (DAMs). Combined analysis of DEMs and DEMis revealed that 21 DEMis negatively regulated 42 DEMs. In addition, when combined with Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of DEMs and DAMs, we found that phenylpropanoid biosynthesis, flavonoid biosynthesis, starch and sucrose metabolism and plant hormone signal transduction played important roles in the alkali stress response. The results of this study further elucidated the regulatory mechanism underlying the plant response to alkali stress and provided valuable information for the breeding of new saline-alkaline tolerance plant varieties.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142002336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory mechanism of strigolactone in tall fescue to low-light stress","authors":"","doi":"10.1016/j.plaphy.2024.109054","DOIUrl":"10.1016/j.plaphy.2024.109054","url":null,"abstract":"<div><p>Strigolactone (SL), a plant hormone derived from carotenoids, has been recognized for its pivotal role in regulating plant growth. Nevertheless, the influence of SL on tall fescue (<em>Festuca arundinacea</em>) under low-light conditions remains unclear. This study aimed to investigate the impact of SL on various aspects of tall fescue, including its morphological characteristics, photosynthesis, levels of antioxidant and concentrations of SL, under low light intensity (LI). The findings showed that GR24, an artificial analog of SL, positively influenced several parameters of tall fescue under LI. In particular, it enhanced the morphological features such as plant height, leaf width, and biomass, while reducing the number of tillers. Furthermore, it improved the efficiency of photosynthetic by enhancing chlorophyll fluorescence and the gas exchange parameters, mitigating cell damage and improving the contents of antioxidants by increasing the levels of antioxidant enzymes and non-enzymatic antioxidant compounds. Moreover, treatment with SL led to elevated concentrations of this hormone and the levels of gene expression in related pathways. Owing to the immaturity of the genetic transformation system in tall fescue, partial validation through transgenic and mutant materials was obtained using Arabidopsis (<em>Arabidopsis thaliana</em>). These findings demonstrate that SL alleviates the physiological indicators of tall fescue under LI stress and enhances its tolerance to shade. Additionally, it suggests that SL may regulate the shade tolerance of tall fescue through the involvement of <em>FaD14</em>.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142009310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights on β-glycan synthases using in vitro GT-array (i-GT-ray) platform","authors":"","doi":"10.1016/j.plaphy.2024.109052","DOIUrl":"10.1016/j.plaphy.2024.109052","url":null,"abstract":"<div><p>Cellulose and hemicellulose are the major structural β-glycan polysaccharides in cell walls of land plants. They are characterized by a backbone of β-(1,3)- and/or β-(1,4)-linked sugars such as glucose, mannose, or xylose. The backbones of these polymers are produced by processive glycosyltransferases (GTs) called synthases having multiple transmembrane domains anchoring them to the membrane. Thus, they are among the most difficult membrane proteins to test <em>in vitro</em> and to purify. Recently, we developed an <em>in vitro</em> GT-array (<em>i</em>-GTray) platform and showed that non-processive type II membrane GTs could be produced <em>via</em> cell-free system in a soluble and active form and tested in this platform. To determine whether <em>i</em>-GT-ray platform is adequate for the production and testing of β-glycan synthases, we tested five synthases involved in cellulose, xyloglucan, (gluco)mannan, and β-(1,3)(1,4)-mixed-linkage glucan synthesis. Our results revealed unsuspected features of these enzymes. For example, all these synthases could be produced in a soluble and active form and are active in the absence of detergent or membrane lipids, and none of them required a primer for initiation of synthesis. All synthases produced ethanol-insoluble products that were susceptible to the appropriate hydrolases (i.e., cellulase, lichenase, mannanase). Using this platform, we showed that <em>At</em>CslC4 and <em>At</em>XXT1 interact directly to form an active xyloglucan synthase that produced xylosylated cello-oligosaccharides (up to three xylosyl residues) when supplied with UDP-Glc and UDP-Xyl. <em>i</em>-GTray platform represents a simple and powerful functional genomics tool for discovery of new insights of synthase activities and can be adapted to other enzymes.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0981942824007204/pdfft?md5=ef92edfbe81567f9f648dae6420d70f5&pid=1-s2.0-S0981942824007204-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142006711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Target of rapamycin coordinates auxin are involved in exogenous melatonin regulated low temperature tolerance in cucumber seedlings","authors":"","doi":"10.1016/j.plaphy.2024.109055","DOIUrl":"10.1016/j.plaphy.2024.109055","url":null,"abstract":"<div><p>Low temperature (LT) is an important environmental factor affecting the growth and yield of plants. Melatonin (MT) can effectively enhance the LT tolerance of cucumber. This study found that LT stress induced the expression of <em>Comt1</em> (<em>caffeic acid O-methyltransferase 1</em>), with the highest expression being about 2-times that of the control. Meanwhile, the content of MT was found to be roughly 63.16% of that in the control samples. Compared with LT treatment alone, exogenous MT pretreatment upregulated the expression levels of <em>TOR (Target of rapamycin), PIN1 (Pin-formed 1), and YUC4 (YUCCA 4)</em>, with maximum upregulations reaching approximately 66.67%, 79.32%, and 42.86%, respectively. These results suggest that MT may modulate the tolerance of cucumber seedlings to LT stress by regulating the expression of <em>TOR</em>, <em>PIN1</em>, <em>and YUC4</em>. In addition, co-treatment with AZD-8055 (a TOR inhibitor) or NPA (N-1-naphthylphthalamic acid, an auxin polar transport inhibitor) and MT attenuated MT-induced resistance to LT stress, leading to higher levels of reactive oxygen species (ROS), reduced antioxidant defense capacity, and increased damage to the membrane system in cucumber seedlings. Concurrently, the content of osmoregulatory substances and the photosynthesis decreased. These results demonstrate that both TOR and auxin were required for MT to alleviate LT-induced damage in cucumber. In summary, the present study demonstrates that TOR and auxin signaling synergistically contribute to alleviating LT damage in cucumber seedlings by exogenous MT. These findings help us understand the function of MT and provide insights into the regulatory network of MT that regulates the LT tolerance of plants.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142049746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar and soil contributions to crop lodging and yield performance - A meta-analysis","authors":"","doi":"10.1016/j.plaphy.2024.109053","DOIUrl":"10.1016/j.plaphy.2024.109053","url":null,"abstract":"<div><p>Applying biochar has beneficial effects on regulating plant growth by providing water and nutrient availability for plants due to its physicochemical characteristics. Nevertheless, it is still unclear how soil and biochar interactions strengthen crop lodging resistance. The objective of the current study was to find out how soil physicochemical conditions and alterations in biochar affect lodging resistance and crop productivity in cereals. To do this, a meta-analysis was carried out using nine groups of effective variables including type of feedstock, pyrolysis temperature, application rate, soil pH, total nitrogen, available phosphorus, potassium, organic matter (OM), and soil texture. Results showed that straw-derived biochar caused the highest positive effect size in the dry weight of biomass (20.5%) and grain yield (19.9%). Also, the lowest lodging index was observed from straw (−8.3%) and wood-based (−5.6%) biochars. Besides, the high application rate of biochar results in the highest positive effect sizes of plant cellulose (8.1%) and lignin content (7.6%). Soils that contain &gt;20 g kg⁻¹ OM, resulted in the highest positive effect size in dry biomass (27.9%), grain yield (30.2%), and plant height (4.7%). Also, fine-textured soil plays an important role in increasing polymers in the anatomical structure of plants. Overall, the strong connection between biochar and soil processes, particularly the availability of OM, could strengthen plants' ability to tolerate lodging stress and contribute to high nutrient efficiency in terms of crop output and cell wall thickening.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142002337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manganese deficiency alters photosynthetic electron transport in Marchantia polymorpha","authors":"","doi":"10.1016/j.plaphy.2024.109042","DOIUrl":"10.1016/j.plaphy.2024.109042","url":null,"abstract":"<div><p>Manganese (Mn) is considered as an essential element for plant growth. Mn starvation has been shown to affect photosystem II, the site of the Mn₄CaO₅ cluster responsible for water oxidation. Less is known on the effect of Mn starvation on photosystem I. Here we studied the effects of Mn deficiency <em>in vivo</em> on redox changes of P700 and plastocyanin (Pc) in the liverwort <em>Marchantia polymorpha</em> using the KLAS-NIR spectrophotometer. Far-red illumination is used to excite preferentially photosystem I, thus facilitating cyclic electron transport. Under Mn starvation, we observed slower oxidation of P700 and a decrease in the Pc signal relative to P700. The lower Pc content under Mn deficiency was confirmed by western blots. Re-reduction kinetics of P700⁺ and Pc⁺ were faster in Mn deficient thalli than in the control. The above findings show that the kinetics studied under Mn deficiency not only depend on the number of available reductants but also on how quickly electrons are transferred from stromal donors via the intersystem chain to Pc⁺ and P700⁺. We suggest that under Mn deficiency a structural reorganization of the thylakoid membrane takes place favoring the formation of supercomplexes between ferredoxin, cytochrome <em>b</em>_<em>6</em><em>f</em> complex, Pc and photosystem I, and thus an enhanced cyclic electron transport.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0981942824007101/pdfft?md5=8e109df2e1d62a74908e1db5eae89393&pid=1-s2.0-S0981942824007101-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rice OsCIPK17-OsCBL2/3 module enhances shoot Na+ exclusion and plant salt tolerance in transgenic Arabidopsis","authors":"","doi":"10.1016/j.plaphy.2024.109034","DOIUrl":"10.1016/j.plaphy.2024.109034","url":null,"abstract":"<div><p>Soil salinity is detrimental to plant growth and remains a major threat to crop productivity of the world. Plants employ various physiological and molecular mechanisms to maintain growth under salt stress. Identification of genes and genetic loci underlying plant salt tolerance holds the key to breeding salt tolerant crops. CIPK-CBL pathways regulate adaptive responses of plants (especially ion transport) to abiotic stresses via fine-tuned Ca²⁺ signal transduction. In this study, we showed that over-expression of <em>OsCIPK17</em> in Arabidopsis enhanced primary root elongation under salt stress, which is in a Ca²⁺ dependent manner. Further investigation revealed that, under salt stress, <em>OsCIPK17</em> transcript level was significantly induced and its protein moved from the cytosol to the tonoplast. Using both Y2H and BiFC, tonoplast-localised OsCBL2 and OsCBL3 were shown to interact with OsCIPK17. Interestingly, over-expressing salt-induced <em>OsCBL2</em> or <em>OsCBL3</em> in Arabidopsis led to enhanced primary root elongation under salt stress. In this process, OsCIPK17 was shown recruited to the tonoplast (similar to the effect of salt stress). Furthermore, transgenic Arabidopsis lines individually over-expressing <em>OsCIPK17</em>, <em>OsCBL2</em> and <em>OsCBL3</em> all demonstrated larger biomass and less Na ⁺ accumulation in the shoot under salt stress. All data combined suggest that OsCIPK17- OsCBL2/3 module is a major component of shoot Na⁺ exclusion and therefore plant salt tolerance, which is through enhanced Na ⁺ compartmentation into the vacuole in the root. <em>OsCIPK17</em> and <em>OsCBL2/3</em> are therefore potential genetic targets that can be used for delivering salt tolerant rice cultivars.</p></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142122181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}