Plant Physiology and Biochemistry最新文献

{"title":"Integrated omics reveals a SmGBF1-SmGDH3 model that facilitates the accumulation of salvianolic acid B under flooding","authors":"Zhizhou Zhang ,&nbsp;Yukun Shang ,&nbsp;Haomiao Yu ,&nbsp;SongJiang Dai ,&nbsp;Yuanyuan Jiang ,&nbsp;Xuexue Deng ,&nbsp;Long Wang ,&nbsp;Ruiwu Yang ,&nbsp;Li Zhang","doi":"10.1016/j.plaphy.2025.110252","DOIUrl":"10.1016/j.plaphy.2025.110252","url":null,"abstract":"<div><div>Salvianolic acid B, an active ingredient in <em>Salvia miltiorrhiza</em>, is renowned for its strong antioxidant capacity and significant medicinal value. As a rhizome herb, frequent flooding hinders its yield and quality. Recently, studies on the biosynthesis genes of salvianolic acid B have garnered considerable attention under abiotic stress. However, few reports focus on the impact of flooding on the biosynthesis of salvianolic acid B, and there is limited understanding of the potential mechanisms involved. This work found that the accumulation of salvianolic acid B under flooding stress was primarily due to increased ammonium nitrogen (NH₄⁺). Among the numerous compounds, the substantial accumulation of primary metabolites captured our attention. Transcriptome sequencing further indicated that glutamate played a crucial role in responding to flooding stress. By integrating omics analysis, we constructed a co-expression network of <em>S. miltiorrhiza</em> under flooding stress and identified several candidate genes. The detailed analysis confirmed the role of the glutamate dehydrogenase gene (SmGDH3) in promoting the biosynthesis of salvianolic acid B in response to flooding/NH₄⁺ signals. Subsequently, interaction analysis and transgenic assays revealed that SmGBF1 could bind to the promoter region of <em>SmGDH3</em>, enhancing its expression and ultimately leading to the accumulation of salvianolic acid B. Our results provide insights into the role of SmGBF1-SmGDH3 in the biosynthesis of salvianolic acid B, contributing to the improvement of the salvianolic acid B content of <em>S. miltiorrhiza</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110252"},"PeriodicalIF":5.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the effects of white LED irradiation on postharvest quality and carotenoid biosynthesis in fresh goji Berries: Insights from physiological and transcriptomic analyses","authors":"Zhicheng Yan ,&nbsp;Yafei Li ,&nbsp;Tingting Li ,&nbsp;Caie Wu ,&nbsp;Xiaohong Kou ,&nbsp;Dongying Xu ,&nbsp;Dandan Zhou ,&nbsp;Kaiping Cong ,&nbsp;Gongjian Fan ,&nbsp;Xiaojing Li","doi":"10.1016/j.plaphy.2025.110254","DOIUrl":"10.1016/j.plaphy.2025.110254","url":null,"abstract":"<div><div>The fragile texture of fresh goji berries makes them prone to rapid quality deterioration during storage. This study investigated the effects of white LED irradiation (100 μmol m² s⁻¹) on the postharvest quality of fresh goji berries. Compared to control samples, LED irradiation significantly improved fruit firmness and total phenolic content, preserved color quality and total carotenoid levels, and upregulated carotenoid biosynthesis genes such as <em>LbPSY</em>, <em>LbZ-ISO</em>, <em>LbCRTISO</em>, and <em>LbLCA1</em>. Transcriptomic analysis revealed that LED irradiation enhanced the expression of carotenoid biosynthesis-related genes during early and mid-storage, activated the terpenoid backbone biosynthesis pathway (including the MEP and MVA pathways), and downregulated WRKY transcription factors potentially involved in carotenoid repression. These findings showed that LED irradiation could maintain higher quality attributes and nutritional qualities, especially in terms of color quality and carotenoid levels, thereby improving the fresh goji berries' storability. This study provides a useful way to store fresh goji berries in the future after harvest.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110254"},"PeriodicalIF":6.1,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural variation in the promoter of TaHY5 enhances anthocyanin biosynthesis via upregulated transcription of TaF3H and TaANS in purple grain wheat (Triticum aestivum L.)","authors":"Mengfei Tang ,&nbsp;Jialin Guo ,&nbsp;Ge Yan ,&nbsp;Zhengqing Xie ,&nbsp;Baoming Tian ,&nbsp;Lina Wang ,&nbsp;Gongyao Shi ,&nbsp;Fang Wei ,&nbsp;Gangqiang Cao ,&nbsp;Yulin Han","doi":"10.1016/j.plaphy.2025.110246","DOIUrl":"10.1016/j.plaphy.2025.110246","url":null,"abstract":"<div><div>Purple grain wheat (<em>Triticum aestivum</em> L.) has attracted increasing attention owing to its high anthocyanin content that is beneficial to human health; however, the molecular mechanisms governing its pigmentation remain poorly characterized. In this study, two anthocyanin-related structural genes, <em>TaF3H</em> (<em>TraesCS1D02G319700</em>) and <em>TaANS</em> (<em>TraesCS6D02G004300</em>), and one transcription factor (TF) gene, <em>TaHY5</em> (<em>TraesCS6B02G209600</em>), were significantly upregulated in purple grain wheat. Functional characterization of TaHY5 revealed its nuclear localization and transcription activity, validating its predicted role as a transcriptional regulator. The ectopic expression of <em>TaHY5</em> in tobacco (<em>Nicotiana benthamiana</em>) significantly upregulated endogenous anthocyanin biosynthetic genes <em>NtF3H</em> and <em>NtANS</em>, and promoted anthocyanin accumulation. Moreover, transient silencing of <em>TaHY5</em> in purple grain wheat significantly downregulated the expression of <em>TaF3H</em> and <em>TaANS</em>, decreasing the anthocyanin content in the coleoptiles. Yeast one-hybrid (Y1H) assays reflected that TaHY5 directly binds to the ACE box (with the ACGT motif) on the <em>TaF3H</em> promoter and to the G-box (with the CACGTG motif) on the <em>TaANS</em> promoter, activating the transcription of the corresponding genes. Furthermore, the purple-grain variety of wheat contains more copies of light-responsive ACE-motif within the <em>cis</em>-acting element of <em>TaHY5</em> promoter compared with the white grain wheat; this natural variation results in enhanced transcriptional activity of <em>TaHY5</em>. In conclusion, our findings established that TaHY5 serves as a key regulator of anthocyanin biosynthesis in purple grain wheat, directly activating <em>TaF3H</em> and <em>TaANS</em> to drive anthocyanin biosynthesis.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110246"},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The IbMYB52/IbARF11L-IbDRM1 module negatively regulates the root development of sweetpotato","authors":"Chengyang Li ,&nbsp;Weihan Song ,&nbsp;Dandan Wang,&nbsp;Chen Li,&nbsp;Wei Tang,&nbsp;Runfei Gao,&nbsp;Aicen Zhang,&nbsp;Tianqi Gao,&nbsp;Hui Yan,&nbsp;Xin Wang,&nbsp;Meng Kou,&nbsp;Yungang Zhang,&nbsp;Qiang Li","doi":"10.1016/j.plaphy.2025.110250","DOIUrl":"10.1016/j.plaphy.2025.110250","url":null,"abstract":"<div><div>The formation and development of sweetpotato (<em>Ipomoea batatas</em> (L.) Lam) storage roots directly affect its yield and quality. This process involves a multidimensional interaction of genetic regulation, signal transduction of hormones, environmental response, and cultivation management are all interconnected. The natural auxin indole-3-acetic acid (IAA) plays a crucial role in the formation and root development of sweetpotatoes. Previously, we selected the <em>IbDRM1</em> gene that was strongly upregulated in its progeny crossing with “Meiguohong” named “Xu18-192” (X192) exhibits a significant delay in root expansion but the lower expression in sweetpotato variety “Xuzishu8” (XZ8) exhibits normal roots development. <em>IbDRM1</em> contains a conserved Auxin-repressed domain and is significantly induced by IAA. Compared with the wild-type (WT), overexpression of the <em>IbDRM1</em> gene inhibits root development and reduces sweetpotato yield, accompanied by decreased IAA content throughout the growth period, whereas RNA interference (Ri) lines exhibit opposite effects. Furthermore, RT-qPCR analysis revealed that the expression levels of key genes involved in the IAA signaling pathway and root development were reduced in overexpression (OE) plants. Further analysis revealed that IbMYB52 could directly bind to the <em>IbDRM1</em> promoter and promote its expression. The IAA content of <em>IbMYB52</em> OE plants increased by 14.04 %–23.53 %. Similarly, root development in <em>IbMYB52</em> OE plants was notably inhibited. The auxin response factor IbARF11L interacts with IbMYB52 to enhance the transcription of <em>IbDRM1</em>. This study elucidates the molecular mechanism by which the IbMYB52/IbARF11L-<em>IbDRM1</em> module negatively regulates the root development of sweetpotato, offering potential key genes for genetic breeding to improve root development.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110250"},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An R2R3-MYB transcription factor regulates anthocyanin accumulation in response to temperature signals in Cymbidium ensifolium","authors":"Mengyao Wang ,&nbsp;Tingting Huang ,&nbsp;Zhihong Peng ,&nbsp;Shuting Wang ,&nbsp;Gangmu Wu ,&nbsp;Longwei Xiong ,&nbsp;Siren Lan ,&nbsp;Donghui Peng ,&nbsp;Zhong-jian Liu ,&nbsp;Ye Ai","doi":"10.1016/j.plaphy.2025.110244","DOIUrl":"10.1016/j.plaphy.2025.110244","url":null,"abstract":"<div><div><em>Cymbidium ensifolium</em>, an ornamental plant in China, is prized for its diverse flower colors and high aesthetic value. The flower color of <em>C. ensifolium</em> is significantly affected by temperature. In this research, we examined the impact of temperature on anthocyanin accumulation in the sepals of <em>C. ensifolium</em> (‘Shi Zhang Hong’) through different temperature treatments (T1, 20/15 °C, T2, 28/23 °C, T3, 36/31 °C), combined with metabolomics, transcriptome analysis and various experimental methods, screening for key temperature-responsive genes. The results indicated that high temperatures suppress both red pigmentation and anthocyanin accumulation in sepals, while low temperatures promote this process. Metabolomic analysis indicated that temperature primarily influences sepal coloration by regulating the abundance of cyanidin-3-<em>O</em>-glucoside. Notably, its abundance significantly increases under low temperatures but decreases at high temperatures. <em>CeMYB52</em> was found to be a crucial transcription factor (TF) regulating anthocyanin accumulation by transcriptome analysis, with high expression at low temperatures and low expression at high temperatures. Remarkably, the spatio-temporal expression profiles of <em>CeCHS8</em> and <em>CeGST</em> were aligned with <em>CeMYB52</em>. Through regulating the expression of <em>CeCHS8</em> and <em>CeGST</em>, <em>CeMYB52</em> contributes significantly to the promotion of anthocyanin biosynthesis and transport. Our findings offer a molecular basis for breeding temperature-resilient ornamental plants, contributing to agricultural sustainability under climate change.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110244"},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suaeda salsa adapts to high-salt environments through expanding vessel diameter, activating antioxidant enzymes and strengthening osmotic regulation","authors":"Yanyan Wang ,&nbsp;Tongkai Guo ,&nbsp;Changyan Tian ,&nbsp;Ke Zhang ,&nbsp;Zhenyong Zhao ,&nbsp;Mingfang Hu ,&nbsp;Wenxuan Mai","doi":"10.1016/j.plaphy.2025.110249","DOIUrl":"10.1016/j.plaphy.2025.110249","url":null,"abstract":"<div><div>Halophytes have evolved various mechanisms to adapt to saline conditions. However, their morpho-anatomical changes, along with the root metabolic responses to increased salt stress in saline-alkali soils, remain poorly understood. This study aimed to elucidate the adaptive mechanisms of <em>Suaeda salsa</em> under four saline-alkali soil levels (non-saline; NS, lightly saline; LS, moderately saline; MS, and severely saline; SS) by analyzing their anatomical morphological traits, nutritional components, and root differential metabolites through a combination of imaging and metabolomics technologies. Results showed that increased salt stress markedly enhanced leaf succulence, root vessel diameter, and epidermal thickness. Compared to NS treatment, leaf perimeter increased by 11.48, 28.29 and 47.94 %, respectively; root vessel diameter increased by 11.07, 24.39 and 14.31 % (<em>p</em> &lt; 0.05). Root epidermal thickness increased by 24.34 and 109.37 % in MS and SS treatments, compared to NS treatment (<em>p</em> &lt; 0.05). High salt stress disrupted leaf chloroplast and mitochondrial membranes, as indicated by malondialdehyde (MDA) increases of 32.45, 73.36 and 112.96 % in LS, MS and SS treatments (<em>p</em> &lt; 0.05). Antioxidant enzyme activities (catalase; CAT, peroxidase; POD, superoxide dismutase; SOD) significantly increased in LS, MS, and SS treatments by 49.25–71.89 %, 90.64–278.86 %, and 12.94–116.37 %, respectively (<em>p</em> &lt; 0.05). Elevated soil salinity increased sodium/potassium (Na/K) ratios by 223–518 % in roots, 215–330 % in stems, and 303–524 % in leaves; sodium/calcium (Na/Ca) ratios increased by 99–180 % in roots, 842–1810 % in stems, and 1111–2901 % in leaves. Additionally, root metabolic pathways associated with galactose/alanine, aspartate, and glutamate were markedly enhanced, leading to the upregulation of L-aspartate, β-alanine, glycine, galactinol, glycerol, and galactonic acid. Consequently, halophytes highlight their robust adaptive mechanisms by substantial morphological, physiological, and metabolic adjustments in response to salinity stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110249"},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between mitochondrial respiration metabolism, energy efficiency and ultraweak luminescence in strawberry fruit","authors":"Li Zhang,&nbsp;Yang Bai,&nbsp;Jinli Guo","doi":"10.1016/j.plaphy.2025.110253","DOIUrl":"10.1016/j.plaphy.2025.110253","url":null,"abstract":"<div><h3>Background</h3><div>Ultraweak luminescence (UWL) is a natural phenomenon characterized by extremely low light emission in living organisms. Although it has attracted scientific attention for decades, its underlying mechanisms remain unclear.</div></div><div><h3>Objective</h3><div>This study aimed to elucidate the relationship between mitochondrial respiratory metabolism and UWL in strawberry fruit mitochondria, focusing on how electron transport and oxidative phosphorylation affect UWL intensity.</div></div><div><h3>Methods</h3><div>Mitochondria from the strawberry variety ‘Hongyan' were treated with activators adenosine diphosphate (ADP) and sodium succinate (C₄H₄Na₂O₄) or inhibitors 2,4-Dinitrophenol (DNP) and sodium vanadate (NaVO₄) during ATP synthesis. Comparative analyses assessed mitochondrial respiration, energy parameters, and UWL changes under these treatments.</div></div><div><h3>Results</h3><div>Activator treatments significantly increased respiratory enzyme activities of succinate dehydrogenase (SDH), cytochrome <em>c</em> oxidase (COX) and H⁺-ATPase, respiration rate, respiratory pathway contributions, ATP, ADP, energy charge, and UWL intensity at higher concentrations (<em>P</em> &lt; 0.05). In contrast, inhibitor treatments caused progressive reductions in these indicators and UWL intensity, also reaching significance at higher concentrations. Correlation analysis showed UWL intensity was positively associated with respiration metrics, especially respiration rate and cytochrome pathway contribution (<em>P</em> &lt; 0.05), and strongly correlated with ATP, ADP, and energy charge (<em>P</em> &lt; 0.05 or <em>P</em> &lt; 0.01), while negatively correlated with AMP (<em>P</em> &lt; 0.05). Principal component analysis indicated that energy parameters contributed most to UWL variation, followed by respiration rate and enzyme activities.</div></div><div><h3>Conclusion</h3><div>UWL intensity reflects mitochondrial respiratory metabolism and ATP production, suggesting mitochondria are key organelles in UWL generation. UWL could serve as a potential indicator of mitochondrial function and cellular energy status.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110253"},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salvia miltiorrhiza-derived carbon dots alleviate cadmium stress in flowering Chinese cabbage by suppressing BrTCP9-mediated cadmium transport and reactive oxygen species metabolism","authors":"Hongyi Qin ,&nbsp;Jiajing Zeng ,&nbsp;Xinrui He ,&nbsp;Juan Li ,&nbsp;Dalian Lu ,&nbsp;Bingfu Lei ,&nbsp;Min Zhong ,&nbsp;Yunyan Kang ,&nbsp;Xirong Chai ,&nbsp;Xian Yang","doi":"10.1016/j.plaphy.2025.110240","DOIUrl":"10.1016/j.plaphy.2025.110240","url":null,"abstract":"<div><div>Cadmium stress not only inhibits vegetable growth but also threatens food safety. Carbon dots (CDs) and TCP transcription factors play crucial roles in responding to abiotic stress. As a leafy vegetable, flowering Chinese cabbage exhibits heightened vulnerability to cadmium contamination. However, the molecular mechanism by which CDs may alleviate cadmium stress through TCP-mediated regulation of cadmium transport and reactive oxygen species (ROS) metabolism remains unclear. In this study, we found that <em>Salvia miltiorrhiza</em>-derived CDs (<em>Sm</em>CDs) alleviated cadmium stress in flowering Chinese cabbage in a concentration-dependent manner. An optimal concentration of <em>Sm</em>CDs significantly enhanced cadmium tolerance and plant growth. Furthermore, TCP transcription factor simultaneously induced by cadmium and suppressed by <em>Sm</em>CDs was identified as BrTCP9, while the cadmium transporter and <em>Rboh</em> gene were identified as <em>BrNramp1.2</em> and <em>BrRbohA</em>, respectively. Under cadmium stress, silencing <em>BrTCP9</em>, <em>BrNramp1.2</em>, or <em>BrRbohA</em> markedly suppressed ROS production, reduced both cadmium and malondialdehyde accumulation, and promoted plant growth, with <em>Sm</em>CDs application further enhancing these effects. Intriguingly, <em>BrNramp1.2</em> and <em>BrRbohA</em> were found to mutually regulate each other under cadmium stress. Y1H, LUC, GUS, and EMSA assays confirmed that BrTCP9 directly binds to the promoter of <em>BrNramp1.2</em> and <em>BrRbohA</em>, activating their expression. These results indicated that BrTCP9, <em>BrNramp1.2</em>, and <em>BrRbohA</em> negatively regulated cadmium tolerance, whereas <em>Sm</em>CDs mitigated cadmium stress by suppressing BrTCP9-mediated <em>BrNramp1.2</em> and <em>BrRbohA</em> expressions. This study not only expanded the molecular network of cadmium stress response but also provided theoretical foundation for the application of carbon nanomaterials in agricultural heavy metal remediation.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110240"},"PeriodicalIF":6.1,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Poplar PdRabG3f inhibits root elongation and increases salt tolerance by enhancing endogenous abscisic acid synthesis","authors":"Jiujun Du ,&nbsp;Xinglu Zhou ,&nbsp;Hantian Wei ,&nbsp;Yongxia Bai ,&nbsp;Xueqin Song ,&nbsp;Lei Zhang ,&nbsp;Jianjun Hu","doi":"10.1016/j.plaphy.2025.110233","DOIUrl":"10.1016/j.plaphy.2025.110233","url":null,"abstract":"<div><div>Proteins belonging to the small GTPases family, particularly Rab proteins, are involved in plant root development. The RabG subfamily proteins, in particular, play a role in regulating plant stress responses. This study revealed that the poplar RabG protein PdRabG3f exhibited tissue-specific activity in root epidermis, root hairs, and vascular stele. Functional characterization further demonstrated that overexpression of <em>PdRabG3f</em> in transgenic poplar significantly inhibited root elongation, reducing maximum root length, and fresh root weight, whereas suppression of <em>PdRabG3f</em> enhanced root growth — a phenotype consistent with its spatial expression pattern. In <em>PdRabG3f</em> overexpression lines, elevated endogenous abscisic acid (ABA) levels were observed, which correlated with enhanced ABA sensitivity and further inhibition of root elongation under exogenous ABA treatment. As a result, these lines exhibited improved resilience under salt stress, including higher peroxidase activity, reduced malondialdehyde content, and maintained relative water content. Transcriptome analysis further supported their enhanced salt tolerance, revealing significant upregulation of stress-responsive genes and enrichment in stress response pathways. These findings establish <em>PdRabG3f</em> as a negative regulator of root growth via ABA biosynthesis and highlight its role in enhancing salt tolerance through ABA-dependent stress signaling. This study provides novel insights into Rab protein-mediated hormonal regulation in woody plants, offering potential targets for improving stress resilience in poplar.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110233"},"PeriodicalIF":6.1,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144632955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The PuWRKY29-PuMYB62 module responds to salicylic acid to inhibit the synthesis of stone cells in ‘Nanguo’ pear","authors":"Mingyang Xu,&nbsp;Xuefeng Zhang,&nbsp;Fan Jiang,&nbsp;He Zhang,&nbsp;Ning Yan,&nbsp;Xinyue Li,&nbsp;Guodong Du","doi":"10.1016/j.plaphy.2025.110238","DOIUrl":"10.1016/j.plaphy.2025.110238","url":null,"abstract":"<div><div>Stone cells serve as pivotal determinants of pear fruit quality, with their formation exhibiting a strong correlation with lignification processes. As a phenolic signaling molecule with multiple biological functions, salicylic acid (SA) plays essential roles in regulating fruit growth and developmental processes. Nevertheless, the molecular mechanisms underlying SA-mediated modulation of stone cell differentiation in pears remain poorly characterized. Our investigation revealed that exogenous application of 15 mg L⁻¹ SA decreased lignified stone cell formation in ‘Nanguo’ (<em>Pyrus ussuriensis</em>) pear fruits and significantly downregulating the expression of the lignin biosynthesis-related <em>PuPRX64</em>. mRNA-seq identified two transcription factors, PuWRKY29 and PuMYB62, which were significantly induced by SA. Functional characterization through transient expression assays in pear fruits and stable transgenic calli demonstrated that both factors act as negative regulators of stone cell formation and lignin accumulation. PuWRKY29 binds to the W-box <em>cis-</em>element within the <em>PuMYB62</em> promoter to activate its transcription, while simultaneously forming a protein complex that synergistically represses <em>PuPRX64</em> expression. Collectively, our findings suggest that exogenous SA treatment inhibits stone cell and lignin biosynthesis in pears via the PuWRKY29-PuMYB62 regulatory module.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"228 ","pages":"Article 110238"},"PeriodicalIF":6.1,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}