Plant Physiology and Biochemistry最新文献_第7页

Advantages of compost tea: Promotion of nitrogen influx into the fruit and improvement of fruit nitrogen metabolism in tomato 堆肥茶的优点：促进番茄果实的氮流入，改善果实的氮代谢

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-09 DOI: 10.1016/j.plaphy.2024.109184

{"title":"Advantages of compost tea: Promotion of nitrogen influx into the fruit and improvement of fruit nitrogen metabolism in tomato","authors":"","doi":"10.1016/j.plaphy.2024.109184","DOIUrl":"10.1016/j.plaphy.2024.109184","url":null,"abstract":"<div><div>The use of compost tea is important to improve food safety. However, the effect of compost tea on N uptake and partitioning in tomato is unclear. In this study, we measured temporal and spatial changes in nitrogen content, enzyme activities, and expression levels of nitrogen transporters genes in different organs of tomato treated with five nutrient solutions. The results showed that the expression levels of ammonium transporter protein genes (<em>AMT1s</em>) increased and that of a nitrogen transporters gene (<em>NRT2.1</em>) decreased under treatment with compost tea, which promoted NH<sub>4</sub><sup>+</sup> transport to the fruit and reduced nutrient wastage compared with the response to chemical fertilizers. In addition, the lowermost leaves on the stem showed reduced nitrate content, faster metabolism, and decreased chlorophyll <em>a</em> content, but fruit yield was not increased, in compost tea-treated plants. These changes were dependent on the expression level of the glutamine synthetase gene (<em>GS1.1</em>), which was increased in leaves and decreased in fruit. Compost tea influenced the expression of critical genes in the fruits and leaves, and improved the competitiveness of sexual reproductive growth as a sink for nitrogen. However, the benefits of compost tea were reduced when it was mixed chemical fertilizers. This research establishes a theoretical framework for optimization of organic vegetable cultivation and promoting the widespread production of organic crops.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of temperature on morphology, physiology, and metabolic profile of diazotrophic cyanobacteria inhabiting diverse habitats 温度对栖息于不同生境的重氮蓝藻的形态、生理和代谢特征的影响。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-09 DOI: 10.1016/j.plaphy.2024.109186

{"title":"Effects of temperature on morphology, physiology, and metabolic profile of diazotrophic cyanobacteria inhabiting diverse habitats","authors":"","doi":"10.1016/j.plaphy.2024.109186","DOIUrl":"10.1016/j.plaphy.2024.109186","url":null,"abstract":"<div><div>Global population expansion has increased the demand for food supply and agricultural productivity. Abiotic stressors like temperature have significantly restricted agriculture in cropland and jeopardized food security. Cyanobacteria play a crucial role in fostering sustainable agriculture and ensuring global food security. In the present study, we have assessed the effect of temperatures on diazotrophic free living rice-field and hot-spring cyanobacteria. They were treated to a variable range of temperatures to see the changes in cellular morphology, physiology, and biochemical characteristics. The rise of temperatures induces growth (60 %), total protein (54 %) contents of rice-field cyanobacterium until 25 °C, further treatment results in decline (20 %) at 45 °C. However, growth indices were increased till 35 °C (90 %) in hot-spring cyanobacterium and further treatment did not exhibit a significant decline in the same. However, the reactive oxygen species (ROS) generation and lipid peroxidation (LPO) were higher in rice-field (2.8 and 1.7 fold) as compared to hot-spring cyanobacterium (2.2 and 1.6 fold). In response to temperature, enzymatic antioxidant contents were much higher in hot-spring as compared to rice-field cyanobacterium. Similarly, carotenoid and carbohydrate content was also higher in hot spring (2 fold) as compared to rice-field cyanobacterium (1.5 and 1.2 fold). All these data collectively suggest that hot-spring (<em>Nostoc</em> sp. strain VKB02) has a higher thermoprotective capacity with novel defense mechanisms as compared to rice-field cyanobacterium (<em>Anabaena</em> sp<em>.</em> strain VKB01). These findings contributed to a better understanding of the temperature stress, improvement of agricultural productivity and future welfare of green ecosystems.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation mechanism of exogenous nitric oxide on phenanthrene uptake by ryegrass roots 外源一氧化氮对黑麦草根系吸收菲的调节机制

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-08 DOI: 10.1016/j.plaphy.2024.109185

{"title":"Regulation mechanism of exogenous nitric oxide on phenanthrene uptake by ryegrass roots","authors":"","doi":"10.1016/j.plaphy.2024.109185","DOIUrl":"10.1016/j.plaphy.2024.109185","url":null,"abstract":"<div><div>Polycyclic aromatic hydrocarbons (PAHs) constitute a category of persistent organic contaminants that possess the potential to induce carcinogenic, teratogenic, and mutagenic consequences. Our previous findings have revealed that plant roots actively take up PAHs through co-transport with protons, and auxin can promote PAHs uptake by wheat roots. It remains unclear whether nitric oxide (NO), a signaling molecule involved in numerous physiological processes in plants and downstream of auxin, can affect PAHs uptake by plant roots. In our study, 50 μmol/L sodium nitroprusside (SNP) significantly enhanced phenanthrene uptake after 4 h of exposure. After the addition of SNP (50 μmol/L), the H<sup>+</sup> flux on root surface increased, and H<sup>+</sup>-ATPase activity was activated, indicating that exogenous NO promotes phenanthrene uptake by plant roots via activating H<sup>+</sup>-ATPase. By studying the effects of 50 μmol/L cyclic guanosine monophosphate (cGMP), 5 mmol/L Ca<sup>2+</sup>, and 50 μmol/L adenosine monophosphate (AMP) on phenanthrene uptake by ryegrass roots and measuring root calcium-dependent protein kinases (CDPK) activity, we demonstrated that exogenous NO promotes phenanthrene uptake through the signaling pathway of NO, cGMP, Ca<sup>2+</sup>, CDPK, 14-3-3 protein and H<sup>+</sup>-ATPase. The results contribute significant insights into elucidating the underlying mechanisms of NO modulating PAHs absorption by plant roots, thereby offering crucial strategies for advancing food safety measures and enhancing the phytoremediation potential of soils and waters contaminated with PAHs.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xyloglucan endotransglucosylase-hydrolase 1 is a negative regulator of drought tolerance in barley via modulating lignin biosynthesis and stomatal closure 木聚糖内转糖基酶-水解酶1是大麦耐旱性的负调控因子，它通过调节木质素的生物合成和气孔关闭来提高大麦的耐旱性。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-05 DOI: 10.1016/j.plaphy.2024.109171

{"title":"Xyloglucan endotransglucosylase-hydrolase 1 is a negative regulator of drought tolerance in barley via modulating lignin biosynthesis and stomatal closure","authors":"","doi":"10.1016/j.plaphy.2024.109171","DOIUrl":"10.1016/j.plaphy.2024.109171","url":null,"abstract":"<div><div>The projected increase in drought severity and duration worldwide poses a significant threat to crop growth and sustainable food production. Xyloglucan endotransglucosylase/hydrolases (XTHs) family is essential in cell wall modification through the construction and restructuring of xyloglucan cross-links, but their role in drought tolerance and stomatal regulation is still illusive. We cloned and functionally characterized <em>HvXTH1</em> using genetic, physiological, biochemical, transcriptomic and metabolomic approaches in barley. Evolutionary bioinformatics showed that orthologues of XTH1 was originated from Streptophyte algae (e.g. some species in the Zygnematales) the closest clade to land plants based on OneKP database. <em>HvXTH1</em> is highly expressed in leaves and HvXTH1 is localized to the plasma membrane. Under drought conditions, silencing <em>HvXTH1</em> in drought-tolerant Tibetan wild barley XZ5 induced a significant reduction in water loss rate and increase in biomass, however overexpressing <em>HvXTH1</em> exhibited drought sensitivity with significantly less drought-responsive stomata, lower lignin content and a thicker cell wall. Transcriptome profile of the wild type Golden Promise and <em>HvXTH1</em>-OX demonstrated that drought-induced differentially expressed genes in leaves are related to cell wall biosynthesis, abscisic acid and stomatal signaling, and stress response. Furthermore, overexpressing <em>HvXTH1</em> suppressed both genes and metabolites in the phenylpropanoid pathway for lignin biosynthesis, leading to drought sensitivity of <em>HvXTH1</em>-OX. We provide new insight by deciphering the function of a novel protein HvXTH1 for drought tolerance in cell wall modification, stomatal regulation, and phenylpropanoid pathway for lignin biosynthesis in barley. The function of HvXTH1 in drought response will be beneficial to develop crop varieties adapted to drought.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive studies of the serine carboxypeptidase-like (SCPL) gene family in Carya cathayensis revealed the roles of SCPL4 in epigallocatechin-3-gallate (EGCG) synthesis and drought tolerance 对 Carya cathayensis 中丝氨酸羧肽酶样（SCPL）基因家族的综合研究发现，SCPL4 在表没食子儿茶素-3-棓酸盐（EGCG）合成和抗旱性中发挥作用。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-05 DOI: 10.1016/j.plaphy.2024.109183

{"title":"Comprehensive studies of the serine carboxypeptidase-like (SCPL) gene family in Carya cathayensis revealed the roles of SCPL4 in epigallocatechin-3-gallate (EGCG) synthesis and drought tolerance","authors":"","doi":"10.1016/j.plaphy.2024.109183","DOIUrl":"10.1016/j.plaphy.2024.109183","url":null,"abstract":"<div><div>Hickory (<em>Carya cathayensis</em>) nuts are rich in epigallocatechin-3-gallate (EGCG) with multiple health functions. EGCG also regulates plant growth, development and stress responses. However, research on the synthesis mechanism of EGCG and its function in hickory is currently limited. Herein, 44 serine carboxypeptidase-like (<em>SCPL</em>) members were identified from the hickory genome and classified into three major categories: SCPL-I, SCPL-II, and SCPL-III. In the CcSCPLs-IA branch, <em>CcSCPL3/4/5/8/9/11/13</em> showed differential expression patterns in various tissues, especially with relatively high expression levels in plant roots, female flowers and seed coat. These proteins have a catalytic triad composed of serine (Ser), aspartic acid (Asp) and histidine (His). Ser-His in the triad and arginine (Arg) mediated the docking of CcSCPL3/4/5/11 with 1-<em>O</em>-galloyl-β-<span>d</span>-glucose (βG) and epigallocatechin (EGC), whereas the Asp of the triad did not. CcSCPL4 was further confirmed to promote the synthesis of EGCG in tobacco leaves. CcSCPL4 may function as monomer and be mainly localized within cellular structures outside the nucleus. Notably, the expression level of <em>CcSCPL4</em> significantly changed after drought, cold, and salt stress, with the highest expression level under drought stress. Meanwhile <em>CcSCPL4</em> over-expression could enhance the drought resistance of <em>Saccharomyces cerevisiae</em> and <em>Arabidopsis</em>. This study elucidates key enzymes for EGCG synthesis and their role in drought resistance, providing insights into the EGCG synthesis pathway and molecular breeding of hickory in future.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factor NtMYB59 targets NtMYB12 to negatively regulate the biosynthesis of polyphenols in Nicotiana tabacum 转录因子 NtMYB59 以 NtMYB12 为靶标，负向调节烟草中多酚的生物合成。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-05 DOI: 10.1016/j.plaphy.2024.109181

{"title":"Transcription factor NtMYB59 targets NtMYB12 to negatively regulate the biosynthesis of polyphenols in Nicotiana tabacum","authors":"","doi":"10.1016/j.plaphy.2024.109181","DOIUrl":"10.1016/j.plaphy.2024.109181","url":null,"abstract":"<div><div>MYB12 is a key regulator that has been shown to promote the accumulation of various phenylpropanoid compounds in plants. However, the regulation of <em>MYB12</em> gene is largely unknown. In this study, we found that overexpression of the <em>NtMYB59</em> gene significantly inhibited the accumulation of chlorogenic acid (CGA), flavonols, and anthocyanins in tobacco, while knock-down and knock-out of <em>NtMYB59</em> significantly increased the contents of these polyphenol compounds. Transcriptome analysis between WT and <em>NtMYB59-OE</em> plants revealed several differentially expressed genes (DEGs) encoding crucial enzymes in the phenylpropanoid pathway and the transcription factor NtMYB12. ChIP-seq assay further indicated that <em>NtMYB1</em>2 might be a direct target of NtMYB59. Subsequent yeast one-hybrid, electrophoretic mobility shift assay, and Dual-Luciferase assays confirmed that NtMYB59 directly binds to the promoter of <em>NtMYB12</em> to inhibit its expression. Moreover, loss-function of NtMYB59 significantly promoted the accumulation of flavonols and anthocyanins in <em>ntmyb59</em>, but their contents in <em>ntmyb59/ntmyb12</em> double mutants were significantly lower than that of WT and <em>ntmyb59</em> plants, indicating that the regulation of NtMYB59 on flavonoids biosynthesis depends on the activity of NtMYB12. Our study revealed that NtMYB59 regulates the expression of <em>NtMYB12</em>, and provided new potential strategies for modulating phenylpropanoids biosynthesis in tobacco.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mn-CDF family genes enhance the manganese tolerance of the tea plants (Camellia sinensis) under acidic condition Mn-CDF 家族基因增强了茶树在酸性条件下的耐锰能力。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-04 DOI: 10.1016/j.plaphy.2024.109179

{"title":"Mn-CDF family genes enhance the manganese tolerance of the tea plants (Camellia sinensis) under acidic condition","authors":"","doi":"10.1016/j.plaphy.2024.109179","DOIUrl":"10.1016/j.plaphy.2024.109179","url":null,"abstract":"<div><div>The tea plants cultivated in acidic soils are vulnerable to excessive manganese (Mn), which increases the risk of Mn<sup>2+</sup> toxicity to physiology and development. Mn-cation diffusion facilitator (CDF) family genes have been implicated in regulating Mn homeostasis and tolerance. However, the mechanism of Mn tolerance of tea plants in acidic environments is still unknown. In this study, we initially examined the phenotypic characteristics and Mn contents variability in different tissues of tea plants under various Mn concentration at pH 5 and 4. We observed that tea plants exhibited remarkably high Mn tolerance at pH 4, with Mn accumulation notably elevated in the aboveground tissues under pH 4 condition after 28-day treatment. We found the expression levels of Mn-CDF genes, had different subcellular localization, were tissue-specific and significantly induced by high Mn concentrations at pH 4 condition. Furthermore, the yeast complementation assays indicated that the heterologous expression of Mn-CDF genes restored the growth of a Mn<sup>2+</sup> sensitive yeast strain, <em>Δpmr1</em>. Taken together, these results suggest that Mn-CDF family genes function as Mn transporters to participate in Mn tolerance in acidic environments. This study provides reference for further study on the mechanism of maintaining Mn homeostasis in tea plants under soil acidification.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time live imaging of cytosolic hydrogen peroxide and Ca2+ of Marchantia polymorpha gemmalings reveal immediate initial responses of plant cells triggered by nonthermal plasma irradiation 对马钱子藻细胞膜过氧化氢和 Ca2+ 的实时活体成像揭示了非热等离子体辐照引发的植物细胞即时初始反应

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-03 DOI: 10.1016/j.plaphy.2024.109172

{"title":"Real-time live imaging of cytosolic hydrogen peroxide and Ca2+ of Marchantia polymorpha gemmalings reveal immediate initial responses of plant cells triggered by nonthermal plasma irradiation","authors":"","doi":"10.1016/j.plaphy.2024.109172","DOIUrl":"10.1016/j.plaphy.2024.109172","url":null,"abstract":"<div><div>Cold atmospheric pressure plasma generators capable of generating plasma under normal pressure and temperature conditions have recently been developed, and their biological applications have been extensively studied. Plasma irradiation has been reported to affect plant germination and growth; however, the molecular mechanism underlying these effects and initial cellular responses to plasma irradiation remains poorly understood. To unravel the molecular and cellular mechanisms underlying the effects of plasma irradiation on plants, we have been establishing novel experimental systems using a model liverwort <em>Marchantia polymorpha</em>. We here focused on the initial responses of plant cells to plasma irradiation. To investigate immediate cellular responses following plasma irradiation, we developed a new plasma device that allows irradiation under a microscope. Through integration with live fluorescence imaging, we established an experimental setup to track, the dynamics of intracellular concentration of H<sub>2</sub>O<sub>2</sub> and Ca<sup>2+</sup> as representative initial cellular responses. We revealed that plasma irradiation induced a rapid and transient increase in intracellular concentration of H<sub>2</sub>O<sub>2</sub> and Ca<sup>2+</sup> in <em>Marchantia</em> gemmalings. Pharmacological analyses suggested that the long-lived reactive species, H<sub>2</sub>O<sub>2</sub>, generated by the plasma generator was directly delivered into the plant cells. Competitive inhibitors of Ca<sup>2+</sup> channels abolished the Ca<sup>2+</sup> rise, suggesting that plasma irradiation immediately activate plasma membrane Ca<sup>2+</sup> channel(s) to induce Ca<sup>2+</sup> influx. Importantly, this study marks the inaugural demonstration of real-time monitoring of cytosolic H<sub>2</sub>O<sub>2</sub> and Ca<sup>2+</sup> dynamics in plants, triggered by plasma irradiation.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of biochar amendment on Cd accumulation in Bidens pilosa L: Changing Cd subcellular distribution, cell wall polysaccharide Cd-binding capacity and composition 生物炭修正对 Bidens pilosa L 中镉积累的影响：改变镉的亚细胞分布、细胞壁多糖的镉结合能力和组成。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-03 DOI: 10.1016/j.plaphy.2024.109177

{"title":"The effect of biochar amendment on Cd accumulation in Bidens pilosa L: Changing Cd subcellular distribution, cell wall polysaccharide Cd-binding capacity and composition","authors":"","doi":"10.1016/j.plaphy.2024.109177","DOIUrl":"10.1016/j.plaphy.2024.109177","url":null,"abstract":"<div><div>Biochar can reduce Cd uptake by plants, thereby reducing its biotoxicity, but the mechanisms involved at the subcellular level have not been thoroughly elucidated. In this work, we explored the effect of maize straw biochar on Cd accumulation by <em>Bidens pilosa</em> L. and its mechanism at subcellular levels. After 90 days of potting experiment, the subcellular fractions were extracted by differential centrifugation, and the polysaccharide fractions of root cell walls were extracted by leaching centrifugation, and then the Cd content of each fraction was determined. Results showed that Cd was preferentially distributed in cell walls of three organs. Additionally, biochar addition resulted in a greater distribution of Cd from cell wall to soluble fractions and organelles in stems. These results suggested that cell wall immobilization and intracellular compartmentalization were critical detoxification mechanisms tackling Cd stress with biochar addition of <em>Bidens pilosa</em> L. Pectin was the main sink where Cd was stored. And galacturonic acid content in pectin occupied the highest ratio among the three polysaccharide fractions. After biochar addition, in hemicellulose the change of Cd content was consistent with the change of galacturonic acid content. These results suggested that the galacturonic acid in hemicellulose played an important role in Cd binding. Biochar addition reduced the bioavailability of soil Cd and improved the growth environment, thus inducing <em>Bidens pilosa</em> L. to change the composition of root cell wall in response to Cd stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of boron and cadmium on the metal enrichment and cell wall immobilization capacity of Cosmos bipinnatus 硼和镉对 Cosmosbipinnatus 的金属富集和细胞壁固定能力的协同效应。

IF 6.1 2区生物学

Plant Physiology and Biochemistry Pub Date : 2024-10-03 DOI: 10.1016/j.plaphy.2024.109178

{"title":"Synergistic effects of boron and cadmium on the metal enrichment and cell wall immobilization capacity of Cosmos bipinnatus","authors":"","doi":"10.1016/j.plaphy.2024.109178","DOIUrl":"10.1016/j.plaphy.2024.109178","url":null,"abstract":"<div><div>Cadmium (Cd), as a heavy metal pollutant, can seriously affect plant growth and development. Boron (B), as an indispensable nutrient element, plays an important role in plant growth and cell wall (CW) synthesis. However, the physiological effects of B and Cd on plant growth and the mechanism of Cd chelation by the CW remain unclear. Here, we investigate the effect of exogenous B on Cd accumulation in CW components of <em>Cosmos bipinnatus</em> roots and its mechanism of Cd mitigation. Under B deficiency and single Cd (30 μM) treatments, the growth of <em>C. bipinnatus</em> was significantly inhibited, but the addition of exogenous B significantly increased plant biomass, which increased the Cd content in the underground parts of <em>C. bipinnatus</em> by 20.18% and reduced the Cd translocation factor by 22.22%. Meanwhile, application of exogenous B affected the subcellular Cd content across various Cd forms and alleviated Cd-induced oxidative stress in <em>C. bipinnatus</em>. Additionally, exogenous B and Cd and their mixtures affected the functional groups of the root CW, the proportion of polysaccharide components, the Cd content of polysaccharides, and the polysaccharide uronic acid content of <em>C. bipinnatus</em>. However, B application increased 3-deoxy-oct-2-ulosonic acid content, pectin esterase activity, low esterified pectin content, and its Cd content by 149.52%, 55.69%, 206.38%, and 150.02%, respectively, compared to Cd treatment alone. Thus, our study showed that B mitigates the toxicity of Cd to plants, revealing the effect of B on the physiological aspects of Cd tolerance in plants.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0