Plant Physiology and Biochemistry最新文献

{"title":"Comparative analysis of rapid alkalinization factor peptide-triggered plant immunity in citrus and closely related species","authors":"Wenzhong Shen ,&nbsp;Mengying Yuan ,&nbsp;Lijuan Chen ,&nbsp;Xinxin Zhang","doi":"10.1016/j.plaphy.2025.109941","DOIUrl":"10.1016/j.plaphy.2025.109941","url":null,"abstract":"<div><div>Rapid alkalinization factors (RALFs) are plant-derived, cysteine-rich small peptides that play crucial roles in plant development and immunity. However, their function in citrus immunity remains unexplored. This study systematically identified <em>RALF</em> family members in sweet orange, a cultivated citrus species, and five closely related species known for their strong resistance to citrus diseases. Most species contained 13 RALF genes, and their corresponding RALF orthologs exhibited minimal variation in gene structure, sequence identity, conserved domains, and motifs. However, differences in <em>cis</em>-acting elements within promoter regions led to differences in their expression patterns under biotic stress. Compared with sweet orange, citrus-related species exhibited a stronger and more widespread induction of <em>RALF</em> genes. Additionally, RALF peptides, particularly RALF11, triggered more robust immune responses in these species, including ROS bursts, mitogen-activated protein kinase activation, and the upregulation of defense-related genes. Furthermore, the basal expression levels of <em>CrRLK1Ls</em>, the <em>RALF</em> receptor homologs, were higher in citrus-related species than in sweet orange. Amplifying the <em>RALF-CrRLK1L</em> pathway through <em>CrRLK1L</em> overexpression and <em>RALF</em> peptide application significantly enhanced sweet orange resistance to citrus canker. These findings suggest that citrus-related species have endured selective pressure, leading to greater promoter sequence diversity, which is conducive to fine-tune gene expression in response to environmental challenges.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109941"},"PeriodicalIF":6.1,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Competitive ion uptake and transcriptional regulation as a coordinated dual mechanism of NaCl-mediated cadmium detoxification in Suaeda salsa","authors":"Tian Li ,&nbsp;Xiangna Yang ,&nbsp;Haotian Sun ,&nbsp;Hao Jing ,&nbsp;Sinuo Bao ,&nbsp;Yanfeng Hu ,&nbsp;Wei Shi ,&nbsp;Honglei Jia ,&nbsp;Jisheng Li","doi":"10.1016/j.plaphy.2025.109939","DOIUrl":"10.1016/j.plaphy.2025.109939","url":null,"abstract":"<div><div>Cadmium (Cd), a highly toxic heavy metal, severely inhibits plant growth. Salt alleviates Cd stress in halophytes, however, the molecular mechanisms governing salt-mediated regulation of Cd toxicity remain poorly understood. This study elucidates the protective mechanism of NaCl in Cd-stressed <em>Suaeda salsa</em> seedlings. Cd exposure suppressed seedling growth and induced membrane lipid peroxidation. Conversely, NaCl application not only maintained normal growth but also effectively ameliorated Cd-induced phytotoxicity, potentially through osmotic adjustment mechanisms. Notably, using ion flux analysis, we found that NaCl attenuated Cd²⁺ influx into root epidermal cells, thereby enhancing Cd resistance. Pharmacological inhibition studies confirmed that Na ⁺ competitively inhibits Cd²⁺ uptake through shared channels/transporters. Furthermore, RT-qPCR gene expression profiling revealed that NaCl coordinately activated both ionic compartmentalization and efflux systems through upregulating plasma membrane-localized <em>SsSOS1</em> and tonoplast-associated <em>SsNHX1</em> for Na ⁺ extrusion and vacuolar sequestration, enhancing Cd²⁺ compartmentalization via <em>SsCAX</em> and <em>SsVHA-B</em> mediated transport and maintaining cellular homeostasis through <em>SsHKT1</em> and <em>SsPIP</em>-mediated regulation of water and K⁺ balance, or indirectly inhibit Cd²⁺ influx. It reveals that salt weakens Cd²⁺ influx and enhances Cd tolerance by activating a coordinated gene regulatory network in <em>Suaeda salsa</em>. This finding offers valuable insights into phytoremediation strategies for enhancing crop resilience in Cd-contaminated saline soils.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109939"},"PeriodicalIF":6.1,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MYB transcription factor MdMYB44 positively regulates fruit crispness by directly activating the expression of pectin methylesterase MdMPE3 in apple","authors":"Ling Yang ,&nbsp;Jiali He ,&nbsp;Sijun Qin ,&nbsp;Xiaojing Li ,&nbsp;Xiaodi Wang ,&nbsp;Deguo Lyu","doi":"10.1016/j.plaphy.2025.109936","DOIUrl":"10.1016/j.plaphy.2025.109936","url":null,"abstract":"<div><div>Crispness, a key attribute of fruit texture quality, is a critical determinant of apple commercial value. Pectin, a major component of the cell wall, plays a vital role in maintaining cell structure, turgor pressure, and mechanical support, with pectin methylesterase (PMEs, <span>EC</span> 3.1.1.11) mediating pectin modification during cell wall remodeling. In this study, we identified two genes, <em>MdMYB44</em> and <em>MdMPE3</em> (pectin methylesterase 3), that regulate apple fruit crispness. Through Y1H, EMSA, ChIP-qPCR, and transient expression assays, we demonstrated that the MYB transcription factor MdMYB44 directly enhances the expression of <em>MdMPE3</em> by binding to its promoter. These results indicate that MdMYB44 acts as a positive regulator of fruit crispness by activating <em>MdMPE3</em> transcription. Our findings provide new insights into the molecular mechanisms by which MYB transcription factors and pectin methylesterase influence apple fruit texture, enriching our understanding of the regulation of fruit crispness.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109936"},"PeriodicalIF":6.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought stress disrupts biological nitrogen fixation and starch accumulation compromising growth and yield of cowpea plants","authors":"Andressa Aparecida Rodrigues Melo ,&nbsp;Maycon Anderson Araújo ,&nbsp;Nandhara Angélica Carvalho Mendes ,&nbsp;André Rodrigues Reis","doi":"10.1016/j.plaphy.2025.109931","DOIUrl":"10.1016/j.plaphy.2025.109931","url":null,"abstract":"<div><div>Water scarcity is one of the most important abiotic stresses in biological nitrogen fixation, often limiting the yield of leguminous plants. This study aimed to evaluate the effect of soil moisture on biological nitrogen fixation, cellular osmotic adjustment and cowpea yield. Water restriction started 20 days after sowing with different levels (20, 40, 60, and 80 %) of soil water retention capacity (SWRC). Cowpea plants did not complete life cycle under 20 % SWRC. Plants growing under SWRC less than 60 % decreased stomatal conductance, transpiration, photosynthetic rates and leaf pigments such as chlorophyll and carotenoids. Drought stress caused chlorosis in leaf tissues, reducing sucrose and total sugars in shoots and transport of these metabolites to the roots. Drought stress disrupted number and weight of nodule per plants affecting nitrogen compounds (ureides, nitrate, ammonia and amino acids) accumulation in roots and shoots of cowpea plants. Well-watered plants showed better biological nitrogen fixation, photosynthetic rates and starch accumulation in the leaves. Less stomatal conductance and transpiration rates induced by drought impaired nodulation, biological nitrogen fixation, dry mass of roots and shoots, growth and yield of cowpea plants. These results suggest that stomatal conductance, transpiration rates and nodulation were the main physiological responses limiting growth and yield of cowpea plants grown in soil moisture below than 60 % SWRC.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109931"},"PeriodicalIF":6.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of PP2C gene family in moso bamboo (Phyllostachys edulis) and function analysis of PhePP2CA13","authors":"Qihan Lin,&nbsp;Xin Shan,&nbsp;Qin Si,&nbsp;Yidong Liu,&nbsp;Min Wu","doi":"10.1016/j.plaphy.2025.109929","DOIUrl":"10.1016/j.plaphy.2025.109929","url":null,"abstract":"<div><div>The protein phosphatase 2C (PP2C) gene family plays an important role in plant growth and development and resistance to abiotic stresses. In our research, the PP2C family containing 103 genes was comprised eleven distinct subfamilies in moso bamboo (<em>Phyllostachys edulis</em>). <em>PP2C</em> gene family mainly expand through segmental duplications and basically underwent purifying selection in moso bamboo. PP2CA subfamily, the most extensively studied subfamily, carried 19 members in moso bamboo PP2CA subfamily. The <em>cis</em>-acting elements analysis and expression patterns of <em>PhePP2CA</em> genes indicated their potential functions in response to abiotic stress. Next, we gained the lines of transgenic PhePP2CA13-overexpressing plants. Overexpression of <em>PhePP2CA13</em> significantly decreased tolerance to drought stress, along with a lower germination rate and suppressed root length under drought treatment. Moreover, OE lines also reduced the sensitivity to exogenous ABA in OE lines. More importantly, yeast one-hybrid assays demonstrated that PhePP2CA13 acted downstream of PhebZIP47, and Dual-luciferase assays further showed that the expression of <em>PhePP2CA13</em> was inhibited by PhebZIP47. This study enriched the knowledge of <em>PP2CA</em> genes and provided genetic resources for stress resistant breeding in moso bamboo.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109929"},"PeriodicalIF":6.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CpHSFA2 isolated from a wild native Carica papaya genotype, with potential to confer tolerance to the combined effect of drought stress and heat shock","authors":"Yessica Bautista-Bautista ,&nbsp;Gabriela Fuentes ,&nbsp;Sergio García-Laynes ,&nbsp;Felipe Alonso Barredo-Pool ,&nbsp;Santy Peraza-Echeverria ,&nbsp;Jorge M. Santamaría","doi":"10.1016/j.plaphy.2025.109925","DOIUrl":"10.1016/j.plaphy.2025.109925","url":null,"abstract":"<div><div>Many papaya producing regions are repeatedly affected by drought and high temperatures. In the present study, we investigated the individual effect of heat shock (HS), water deficit stress (WDS) and the combined effect of both types of stress (WD + HS), on the physiological performance of two contrasting papaya genotypes (Maradol and Wild). In all cases, water relations, membrane integrity, gas exchange, photochemical state of PSII and RELs of three <em>Carica papaya</em> transcription factors (<em>CpHsfA1d</em>, <em>CpHsfA2</em> and <em>CpHsfB3</em>, in both a Wild-native genotype collected from an undisturbed site in its center of origin (Yucatán, Mexico; Wild), as well as in a commercial cultivar (Maradol). Results showed that both papaya genotypes have different physiological and molecular mechanisms to cope with individual stress and combined stresses. Wild (W) genotype exhibited greater tolerance to the three types of stresses than the commercial genotype (M), which correlates with the fact that W also showed higher relative expression levels (REL) in the three <em>CpHsf</em> studied: <em>CpHsfA1d</em>, <em>CpHsfA2</em> and <em>CpHsfB3</em> than M. REL of <em>CpHsfA2</em> was particularly high in the HS and in the combined WD + HS treatment, as well as during the recovery phase of the WDS treatment. CpHSFA2 was then selected for further analysis of subcellular localization, finding that it accumulates in the membrane and nucleus. Taken together, it seems that <em>CpHsfA2</em> plays an important role in the response to HS and WD + HS stress. Therefore, <em>CpHsfA2</em> gene from the W genotype could be important to eventually improve tolerance to high temperatures and drought in commercial papaya cultivars.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109925"},"PeriodicalIF":6.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-induced CsCV triggers chloroplast degradation by destabilizing photosystem proteins in tea plant","authors":"Xin Zhang ,&nbsp;Xiaobei Huang ,&nbsp;Zhijun Wu","doi":"10.1016/j.plaphy.2025.109926","DOIUrl":"10.1016/j.plaphy.2025.109926","url":null,"abstract":"<div><div>Excess light induces chloroplast degradation in plants, leading to decreased photosynthetic efficiency and an albino leaf phenotype. However, the molecular mechanism underlying this process remains unclear, especially in perennial crops like tea plant. This study investigated the effects of relatively strong light (SL, 240 μmol m⁻²·s⁻¹) on chloroplast ultrastructure and metabolites in the light-sensitive tea germplasm Nanchuan Dachashu (<em>Camellia nanchuanica</em>). Continuous exposure to SL resulted in abnormal chloroplast structure characterized by extensive vacuolation. SL also significantly decreased the levels of chlorophyll (−60.30 %), carotenoids (−88.29 %), free amino acids (−23.97 %), and caffeine (−41.15 %) compared to relatively weak light (WL, 15 μmol m⁻²·s⁻¹). Transcriptome analysis and RT-qPCR revealed that the chloroplast vesiculation gene <em>CsCV</em> was significantly up-regulated under SL, with promoter analysis showing more light-responsive elements in <em>CsCV</em> compared to another light-responsive gene, <em>CsNBR1</em>. Overexpression of <em>CsCV</em> in <em>Arabidopsis</em> caused stunted growth and accelerated leaf senescence, with the most affected line showing decreases in chlorophyll and carotenoid contents of 24.97 % and 17.39 %, respectively. Conversely, silencing <em>CsCV</em> in tea plants using antisense oligodeoxynucleotides (asODNs) for 3 days increased chlorophyll and carotenoid levels by 15.98 % and 18.35 %, respectively. Bimolecular fluorescence complementation (BiFC) assays and in protein-protein docking simulations demonstrated that CsCV interacts with the photosystem proteins CsLhca1, CsLhcb4, and CsPsaL through its conserved C-terminal region, suggesting CsCV may trigger chloroplast degradation by destabilizing the photosynthetic apparatus under SL. These findings provide mechanistic insights into light-induced chloroplast degradation in tea plants and highlight <em>CsCV</em> as a potential target for improving crop stress tolerance.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109926"},"PeriodicalIF":6.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomics and metabolomics analyses reveal jasmonic acid metabolic pathways for improving the chilling tolerance in cotton seedlings","authors":"Luyao Wang ,&nbsp;Yaping Jiang ,&nbsp;Yupeng Hao ,&nbsp;Li Yu ,&nbsp;Shengjun Zhao ,&nbsp;Hongyu Wu ,&nbsp;Xuan Long ,&nbsp;Zhiyuan Zhang ,&nbsp;Ting Zhao ,&nbsp;Shiwei Geng ,&nbsp;Xueying Guan","doi":"10.1016/j.plaphy.2025.109935","DOIUrl":"10.1016/j.plaphy.2025.109935","url":null,"abstract":"<div><div>Cotton (<em>Gossypium</em> spp.) originated in tropical and subtropical regions, spreading to higher latitudes through domestication while retaining thermophilic characteristics. Xinjiang, a major cotton-producing area in China, frequently experiences ‘late spring cold snaps’ due to its location, causing chilling injury during critical sowing periods. Current research on cotton chilling stress primarily focuses on physiological studies such as evaluations of chilling stress and biochemical indices but lacks systematic investigation into the difference among varieties. Phenotypic screening across seed germination, cotyledon, and seedling stages identified upland cotton (<em>Gossypium hirsutum</em>) cultivar, Junmian1 exhibits superior cold tolerance relative to the sensitive genotype C1470. Under chilling stress, Junmian1 protects chloroplasts and other cellular structures in its first true leaf to survive the chilling stress. Weighted gene co-expression network analysis (WGCNA) analysis pinpointed Module Brown as a chilling-tolerance responsive hub, with subsequent validation via virus-induced gene silencing (VIGS) confirming the regulatory roles of <em>GhRBL</em> (Ribulose-bisphosphate carboxylase), <em>GhGI</em> (GIGANTEA), and lncRNA <em>MSTR.1631</em> in cold tolerance. Additionally, integrated metabolomic and transcriptomic analyses demonstrated that jasmonic acid plays a crucial role in enhancing cotton's chilling tolerance at seedling stage. The primary difference in chilling tolerance between Junmian1 and C1470 is attributed to the signaling efficiency of the jasmonic acid synthesis and metabolism pathways. These findings establish JA metabolic engineering as a viable approach for enhancing cold resilience in early-stage cotton seedlings.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109935"},"PeriodicalIF":6.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription factor WUSCHEL-related homeobox (WOX) underground revelations: Insights into plant root development","authors":"Yuzheng Deng ,&nbsp;Yongjie Zhu ,&nbsp;Wanyi Su ,&nbsp;Meiling Zhang ,&nbsp;Weibiao Liao","doi":"10.1016/j.plaphy.2025.109928","DOIUrl":"10.1016/j.plaphy.2025.109928","url":null,"abstract":"<div><div>Plant roots are essential for nutrient and water uptake and play a crucial role in plant growth and development. The development of roots is a complex process regulated by numerous factors, among which transcription factors (TFs) like WUSCHEL-related homeobox (WOX) have an essential function. The importance of WOXs in root development cannot be overstated. They act as key regulators in maintaining the balance between cell proliferation and differentiation and ensure the proper formation and function of root tissues. This review comprehensively presents the roles of WOXs in various root development aspects across multiple plant species, including primary, lateral, adventitious, and crown root development, as well as root hair, rhizoid formation, <em>de novo</em> root regeneration, and root apical meristem maintenance. We also discuss how WOXs regulate root development through various mechanisms in different plant species. Overall, this review provides comprehensive insights into the complex regulatory networks governing plant root growth and the importance of WOXs therein. Understanding WOXs in root development can help improve crop root architecture and stress tolerance and provide insights into the regulatory networks of plant root growth, contributing to plant breeding and agricultural productivity.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109928"},"PeriodicalIF":6.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myosin XI is required for boron transport under boron limitation via maintenance of endocytosis and polar localization of the boric acid channel AtNIP5;1","authors":"Haiyang Liu ,&nbsp;Keita Muro ,&nbsp;Riku Chishima ,&nbsp;Junpei Takano ,&nbsp;Motoki Tominaga","doi":"10.1016/j.plaphy.2025.109938","DOIUrl":"10.1016/j.plaphy.2025.109938","url":null,"abstract":"<div><div>Myosin XI plays a major role in cytoplasmic streaming and is essential for intracellular transport. Here, we investigated the physiological roles of myosin XI in nutrient transport using double (<em>2ko</em>) and triple (<em>3ko</em>) myosin XI knockout mutants of <em>Arabidopsis thaliana</em>. The results revealed that the mutants exhibited more severe boron deficiency phenotypes under boron-limiting conditions, and the boron concentration in the aerial parts of mutant plants was lower than that in the wild-type. Microscopic analysis demonstrated a reduction in general endocytosis and abolishment of NIP5; 1's polar localization in <em>2ko</em> and <em>3ko</em> plants. Overall, these results indicate that myosin XI is necessary for proper boron transport via the maintenance of the endocytic pathway and NIP5; 1's polar localization.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"224 ","pages":"Article 109938"},"PeriodicalIF":6.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}