Genome-wide analysis of phasiRNAs in tea plant reveals a miR482k-PHAS regulatory pathway in response to gray blight

IF 5.7 2区 生物学 Q1 PLANT SCIENCES
Changhao Chen , Guodong Li , Lei Wang , Kongze Wei , Jia Yu , Zhu Liu , Weihua Sun , Yuxin Chu , Attila Molnar , Yue Fei
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Abstract

The tea plant (Camellia sinensis) is an important beverage crop, with substantial economic value and proven health benefits. Its predominant distribution in tropical and subtropical regions exposes it to numerous pathogens, making disease resistance crucial for tea cultivation. Phased secondary small interfering RNAs (phasiRNAs) are a class of small non-coding RNAs, generated in a phased manner from specific genomic regions termed phasiRNA-producing loci (PHAS loci). PhasiRNAs have been demonstrated to participate in disease response across various plant species. However, the disease resistance function of phasiRNAs in tea plants remains unexplored. Here, we present a genome-wide characterization of PHAS loci and phasiRNA biogenesis pathways in Camellia sinensis. Using sRNAminer, we analyzed all publicly available tea small RNA sequencing data, identifying 305 PHAS loci and four highly conserved phasiRNA biogenesis pathways in tea plants. Among these, a miR482/2118-triggered 21-nt phasiRNA biogenesis pathway was investigated in detail. We identified 16 members of the miR482/2118 family in tea plants, with miR482k exhibiting significantly higher expression levels. Pathogen infection studies showed that a miR482k-targeted PHAS locus regulated the disease resistance gene CsCAR through phasiRNA production. Further analysis across tea cultivars revealed that the activity of this phasiRNA biogenesis pathway correlates with their disease resistance levels. These findings highlight the critical role of the miR482k-PHAS pathways in disease resistance mechanisms, providing new insights into how phasiRNAs contribute to tea plant immunity and defense responses.

Abstract Image

茶树相rna的全基因组分析揭示了miR482k-PHAS调控通路对灰枯病的响应
茶树(Camellia sinensis)是一种重要的饮料作物,具有巨大的经济价值和已被证实的保健益处。它主要分布在热带和亚热带地区,使其暴露于许多病原体,使抗病对茶叶种植至关重要。阶段性次级小干扰rna (phasiRNAs)是一类小的非编码rna,由称为phasirna - production loci (phasas loci)的特定基因组区域分阶段产生。PhasiRNAs已被证明参与多种植物的疾病反应。然而,phasirna在茶树中的抗病功能尚不清楚。在这里,我们提出了一个全基因组的特征,在山茶中phasas位点和phasiRNA的生物发生途径。我们使用sRNAminer分析了所有公开的茶叶小RNA测序数据,鉴定出茶树中305个phasas位点和4个高度保守的phasiRNA生物发生途径。其中,我们详细研究了mir482 /2118触发的21-nt phasiRNA生物发生途径。我们在茶树中鉴定出miR482/2118家族的16个成员,其中miR482k的表达水平明显较高。病原体感染研究表明,靶向mir482k的PHAS位点通过产生phasiRNA调控抗病基因CsCAR。进一步的分析表明,这种phasiRNA生物发生途径的活性与其抗病水平相关。这些发现突出了miR482k-PHAS通路在抗病机制中的关键作用,为phasiRNAs如何促进茶树免疫和防御反应提供了新的见解。
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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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