在拟南芥中,赖氨酸-组氨酸转运蛋白4 (Lysine-Histidine Transporter 4, AtLHT4)促进水仙素(一种生物碱)的吸收

IF 5.7 2区 生物学 Q1 PLANT SCIENCES
Junjie Li , Zhenyu Cheng , Ruiqing Shi , Chang Qi , Ziyu Liu , Meiyun Pu , Juan Qin , Shengwang Wang , Hao Sun , Haoxuan Pan , Xiaoqi He , Xiaomin Wang , Yurong Bi , Xiaofan Na
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引用次数: 0

摘要

已知赖氨酸-组氨酸转运蛋白(LHT)家族成员介导植物的氨基酸摄取、氮获取和对非生物和生物胁迫的反应。然而,它们在响应其他植物源代谢物方面的潜在作用在很大程度上仍未被探索。在这里,我们确定了AtLHT4是拟南芥对水仙素(NCS)敏感的关键因素,水仙素是一种来自Amaryllidaceae家族的生物碱。通过遗传作图和功能分析,我们发现AtLHT4功能的缺失显著增强了植物对NCS的抗性,而其他AtLHT成员的突变没有明显的影响。LC-MS分析显示,暴露4小时后,lht4突变体在根中积累的NCS减少了70%,而AtLHT4过表达增加了NCS的积累。与载体对照相比,异源表达AtLHT4的酵母细胞也积累了更高的细胞内NCS浓度,证实了其在介导NCS摄取中的作用。空间表达分析表明,atht4主要表达于种子、根分生组织区、维管组织、花托和叶柄端,可能在根生长调控和NCS再分配中发挥作用。值得注意的是,外源施用特定氨基酸(包括Gly、Glu、Asp、Phe、Asn和arg)部分或完全缓解了ncs诱导的野生型和light - 4-2幼苗根系生长抑制,这表明氨基酸与生物碱信号传导或摄取途径之间可能存在相互作用。总之,我们的发现揭示了AtLHT4在生物碱摄取中的特殊作用,并扩展了LHT家族在植物中氨基酸运输之外的已知功能库。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lysine-Histidine Transporter 4 (AtLHT4) facilitates the uptake of narciclasine, a bioactive alkaloid, in Arabidopsis
Members of the Lysine-Histidine Transporter (LHT) family are known to mediate amino acid uptake, nitrogen acquisition, and responses to abiotic and biotic stresses in plants. However, their potential roles in responding to other plant-derived metabolites remain largely unexplored. Here, we identify AtLHT4 as a key contributor to Arabidopsis sensitivities to narciclasine (NCS), a bioactive alkaloid derived from the Amaryllidaceae family. Through genetic mapping and functional analyses, we found that loss of AtLHT4 function significantly enhances plant resistance to NCS, while mutations in other AtLHT members have no detectable effect. LC-MS analysis showed that lht4 mutants accumulate ∼70 % less NCS in roots after 4 h of exposure, whereas AtLHT4 overexpression increases NCS accumulation. Yeast cells heterologously expressing AtLHT4 also accumulated higher intracellular concentrations of NCS compared to vector controls, confirming its role in mediating NCS uptake. Spatial expression analysis revealed that AtLHT4 is predominantly expressed in seeds, the root meristematic zone, vascular tissues, floral receptacles, and silique ends, suggesting potential roles in root growth regulation and NCS redistribution. Notably, exogenous application of specific amino acids—including Gly, Glu, Asp, Phe, Asn, and Arg—partially or fully alleviated NCS-induced root growth inhibition in both wild-type and lht4-2 seedlings, indicating possible interactions between amino acid and alkaloid signaling or uptake pathways. Together, our findings uncover a specific role of AtLHT4 in alkaloid uptake and expand the known functional repertoire of the LHT family beyond amino acid transport in plants.
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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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