Characterization of four terpene synthase genes involved in nerolidol and linalool biosynthesis in Santalum album leaves induced by methyl jasmonate

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-10-01 DOI:10.1016/j.plaphy.2025.110571

Yuqing Wang , Chuting Wu , Yuping Xiong , Zhan Bian , Yunfei Yuan , Yongxia Jia , Jaime A. Teixeira da Silva , Guohua Ma , Xinhua Zhang

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引用次数: 0

Abstract

Santalum album is highly valued for its fragrant essential oil from heartwood. Volatile terpenoids involved in aroma formation in plants can be emitted in response to a variety of environmental stresses. However, the regulatory mechanisms underpinning the response of S. album to external stresses are not yet known. In this study, the regulatory mechanism of S. album leaves was investigated after the application of methyl jasmonate (MeJA), a stressor. Eight classes of volatile organic compounds were identified in S. album leaves, including terpenes, aldehydes, alcohols, ketones, esters, benzenoids, alkanes and heterocyclic compounds. In total, 15 terpenoids, such as ocimene, linalool, nerolidol and α-farnesene, were considerably induced 6 h after MeJA treatment, amounting to 38.01 % of all volatiles. Notably, transcript levels of the genes in the MVA pathway were enhanced by 2- to 8-fold after 6 h of MeJA treatment compared to the control. Exogenously applied MeJA resulted in the significant upregulation of ten terpene synthase (SaTPS) genes. In vitro enzyme activity assays confirmed that four SaTPS recombinant proteins converted substrates into (E)-nerolidol and linalool. Overexpression of the four SaTPS genes produced (E)-nerolidol, (Z)-nerolidol and linalool in sandalwood callus. These results suggest that the four SaTPS genes have the ability to synthesize nerolidol and linalool in S. album leaves in response to MeJA treatment. This study provides insight into the regulatory mechanism of the biosynthesis of terpenoids in S. album in response to an environmental stress.

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茉莉酸甲酯诱导檀香叶合成神经樟醇和芳樟醇的4个萜类合成酶基因的研究。

檀香相册因其芳香的心材精油而备受推崇。参与植物香气形成的挥发性萜类化合物可以在各种环境胁迫下释放出来。然而，支持S. album对外部压力反应的调节机制尚不清楚。本研究以茉莉酸甲酯（MeJA）为胁迫源，研究了茉莉酸甲酯（MeJA）对野牡丹叶片的调控机制。经鉴定，白菖蒲叶中含有萜类、醛类、醇类、酮类、酯类、苯类、烷烃和杂环化合物等8类挥发性有机化合物。MeJA处理6 h后，共有15种萜类化合物被显著诱导，分别为山香烯、芳樟醇、神经樟醇和α-法尼烯，占总挥发物的38.01%。值得注意的是，与对照组相比，MeJA处理6小时后，MVA途径中基因的转录水平提高了2- 8倍。外源施加MeJA导致10萜烯合成酶（SaTPS）基因显著上调。体外酶活性测定证实，四种SaTPS重组蛋白可将底物转化为(E)-神经樟醇和芳樟醇。四种SaTPS基因的过表达产生檀香愈伤组织中(E)-橙花醇、(Z)-橙花醇和芳樟醇。这些结果表明，在MeJA处理下，4个SaTPS基因具有在野蔷薇叶片中合成神经樟醇和芳樟醇的能力。本研究揭示了南芥在环境胁迫下萜类生物合成的调控机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.