Integrated transcriptomic analyses reveals LpmiR397 and caffeic acid play positive roles in drought and heat stresses tolerance in perennial ryegrass

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-09-29 DOI:10.1016/j.plaphy.2025.110572

Mingzhi Xu , Yanan Gao , Qinying Zhou , Yuzhou Hou , Feng Yuan , Xu Guan , Yanrong Liu , Wanjun Zhang

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引用次数: 0

Abstract

Perennial ryegrass, is an important turf and pasture grass, often faces stagnant growth and even death in summer due to heat and/or drought stress. Highly heterogeneous genomes and the difficulty of performing stable genetic transformation create challenges for the study of drought- and heat-tolerant gene function. In this study, we sequenced full-length transcripts of perennial ryegrass and identified a total of 24,782 transcripts, including 15,330 new transcripts of known genes and 1504 transcripts of novel genes. In RNA-seq, 16,919 differentially expressed mRNAs (DE-mRNAs) appeared in heat treatment and 1063 DE-mRNAs appeared in drought treatment, which significantly enriched in phenylalanine metabolic pathway and triggers to produce caffeic acid, an intermediate product produced during lignin synthesis. Further study showed that exogenous application of caffeic acid significantly enhanced drought and heat tolerance in ryegrass. By miRNA-seq, 118 known miRNAs and 568 newly predicted miRNAs were detected, and 120 differentially expressed miRNAs (DE-miRNAs) appeared in heat treatment and 15 DE-miRNAs appeared in the drought treatment. 22 miRNA-mRNA regulatory networks response to drought and/or heat treatment. Among them, LpmiR397 showed a negative response to heat treatment and was predicted to cleavage, LONELY GUY (LpLOG), a new target gene that regulating cytokinin content and metabolism. Further, we confirmed that miR397 could cleavage the LpLOG mRNA, and blocking LpmiR397 by using AS-ODN to reduce perennial ryegrass heat tolerance and increased its cytokinin content. This study provides a basis for genomic studies of perennial ryegrass and key molecular pathways for coping with heat and drought and demonstrates the positive effects of caffeic acid and LpmiR397 in improving heat tolerance of perennial ryegrass and the function of low concentrations of caffeic acid in improving drought tolerance.

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综合转录组学分析表明，LpmiR397和咖啡酸在多年生黑麦草的抗旱性和耐热性中起积极作用。

多年生黑麦草是一种重要的草皮和牧草，在夏季由于高温和/或干旱胁迫，经常面临生长停滞甚至死亡。高度异质性的基因组和难以进行稳定的遗传转化，为研究耐旱和耐热基因功能带来了挑战。在本研究中，我们对多年生黑麦草的全长转录本进行了测序，共鉴定出24,782个转录本，其中已知基因的新转录本15,330个，新基因的转录本1504个。在RNA-seq中，热处理中出现16919个差异表达mrna (de - mrna)，干旱处理中出现1063个差异表达mrna，它们在苯丙氨酸代谢途径中显著富集，并触发木质素合成过程中的中间产物咖啡酸的产生。进一步研究表明，外源施用咖啡酸显著提高了黑麦草的耐旱性和耐热性。通过miRNA-seq，共检测到118个已知mirna和568个新预测mirna，其中120个差异表达mirna （de - mirna）出现在热处理中，15个差异表达mirna出现在干旱处理中。22 miRNA-mRNA调控网络对干旱和/或热处理的响应。其中，LpmiR397对热处理表现出负反应，并预测会裂解调控细胞分裂素含量和代谢的新靶基因LONELY GUY （LpLOG）。进一步，我们证实miR397可以切割LpLOG mRNA，通过AS-ODN阻断LpmiR397降低多年生黑麦草的耐热性，增加其细胞分裂素含量。本研究为多年生黑麦草的基因组研究和应对高温干旱的关键分子途径提供了基础，并证明了咖啡酸和LpmiR397在提高多年生黑麦草耐热性中的积极作用以及低浓度咖啡酸在提高耐旱性中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.