Pseudomonas syringae infection causes metabolic changes in tomato leaves

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-09-12 DOI:10.1016/j.plaphy.2025.110521

Chunyan Chen, Jiahua Ye, Keke Zhao, Mingyang Hao, Fangfang Ma, Zhilong Bao

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Abstract

Pathogen infection requires the reprogramming of host metabolism to assist the pathogen growth and stimulate host defense responses. Repression of photosynthesis is a common phenomenon during the pathogen infection, while the changes of primary metabolic pathways and carbohydrates are seldomly studied. This study aims to investigate the temporal changes of physiological parameters and primary metabolites in tomato leaves infected with Pseudomonas syringae pv. Tomato DC3000 (Pst DC3000). Pathogen infection triggered the reduction of pigments and starch accumulation. The production of reactive oxygen species and malondialdehyde are enhanced, and the acitvities of antioxidative enzymes are reduced after the pathogen infection. Pathogen induces the leaf cell enlargement and enhanced endoreduplication. Targeted metabolomics using GC-MS and LC-MS/MS reveals that metabolites in photosynthesis, sugar biosynthesis and TCA cycle are significantly increased between 24 and 36-h post inoculation (hpi). Overall, metabolites in photosynthesis, sugar biosynthesis and TCA cycle are more responsive to the pathogen infection than other metabolic pathways. Moreover, RNA-seq data in Pst DC3000-resistant and susceptible plants reveal that majority genes involved in Calvin cycle, photorespiration, sucrose and starch biosynthesis and TCA cycle have opposite transcriptional patterns after the pathogen infection. Our study illustrates drastic fluctuations of host primary metabolism during the bacterial pathogen infection, suggesting that metabolic engineering on photosynthesis, sugar biosynthesis and TCA cycle may enhance plant disease resistance.

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丁香假单胞菌感染引起番茄叶片代谢变化

病原体感染需要宿主代谢的重编程来帮助病原体生长和刺激宿主的防御反应。光合作用的抑制是病原菌侵染过程中常见的现象，而对主要代谢途径和碳水化合物的变化研究较少。本研究旨在研究丁香假单胞菌侵染番茄叶片后生理参数和主要代谢物的变化。番茄DC3000 （Pst DC3000）。病原菌感染导致色素和淀粉积累减少。病原菌感染后活性氧和丙二醛的生成增强，抗氧化酶活性降低。病原菌诱导叶片细胞增大，内复制增强。利用GC-MS和LC-MS/MS进行的目标代谢组学分析显示，接种后24 ~ 36 h，光合作用、糖生物合成和TCA循环代谢产物显著增加。总的来说，光合作用、糖生物合成和TCA循环中的代谢物比其他代谢途径对病原体感染的反应更敏感。此外，Pst dc3000抗性和易感植物的RNA-seq数据显示，病原菌感染后，参与卡尔文循环、光呼吸、蔗糖和淀粉生物合成以及TCA循环的大多数基因的转录模式相反。本研究揭示了病原菌侵染过程中寄主初级代谢的剧烈波动，提示对光合作用、糖生物合成和TCA循环进行代谢工程可以增强植物的抗病性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.