{"title":"DcMYB30通过调节类黄酮的生物合成而负向影响石斛的抗旱性","authors":"Chenning Zhao , Hongyan Hou , Junxia Wu , Yanqin Zhu , Qingsong Shao , Aimin Lv","doi":"10.1016/j.plaphy.2025.110199","DOIUrl":null,"url":null,"abstract":"<div><div>Drought stress is a severe abiotic stress, limiting the plant growth, development and yield. MYB transcription factor family plays a crucial role in plants response to adversity stress, particularly drought. However, a comprehensive analysis of the MYB gene family in <em>Dendrobium catenatum</em> remains limited, especially regarding the functions of two-repeat MYB proteins in response to drought stress and their regulation of flavonoid biosynthesis and accumulation. Here, severe drought stress inhibited the growth of <em>D. catenatum</em> and decreased polysaccharides and flavonoid contents. This study identified 174 MYB genes and characterized their phylogenetic relationships, protein profiles, and expression patterns. Co-expression analysis and transient expression assay revealed that an R2R3-MYB DcMYB30 was a key regulator in drought response and flavonoid synthesis in <em>D. catenatum</em>. DcMYB30 was found to localize in the nucleus and was down-regulated by drought stress. In both <em>DcMYB30</em>-overexpressing <em>Nicotiana benthamiana</em> or <em>D. catenatum</em>, flavonoid content decreased and transcript levels of multiple flavonoid biosynthetic enzyme-coding genes were downregulated. According to these findings, it is proposed that DcMYB30 may negatively regulate flavonoid biosynthesis by down-regulating flavonoid pathway enzyme-coding genes (e.g., 4CL), thereby reducing the flavonoid biosynthesis or accumulation and drought tolerance in <em>D. catenatum</em>. This study provides fundamental insights for characterizing the physiological roles of two-repeat MYB transcription factors in plant stress responses.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"227 ","pages":"Article 110199"},"PeriodicalIF":5.7000,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DcMYB30 negatively function in drought tolerance of Dendrobium catenatum by modulating flavonoid biosynthesis\",\"authors\":\"Chenning Zhao , Hongyan Hou , Junxia Wu , Yanqin Zhu , Qingsong Shao , Aimin Lv\",\"doi\":\"10.1016/j.plaphy.2025.110199\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Drought stress is a severe abiotic stress, limiting the plant growth, development and yield. MYB transcription factor family plays a crucial role in plants response to adversity stress, particularly drought. However, a comprehensive analysis of the MYB gene family in <em>Dendrobium catenatum</em> remains limited, especially regarding the functions of two-repeat MYB proteins in response to drought stress and their regulation of flavonoid biosynthesis and accumulation. Here, severe drought stress inhibited the growth of <em>D. catenatum</em> and decreased polysaccharides and flavonoid contents. This study identified 174 MYB genes and characterized their phylogenetic relationships, protein profiles, and expression patterns. Co-expression analysis and transient expression assay revealed that an R2R3-MYB DcMYB30 was a key regulator in drought response and flavonoid synthesis in <em>D. catenatum</em>. DcMYB30 was found to localize in the nucleus and was down-regulated by drought stress. In both <em>DcMYB30</em>-overexpressing <em>Nicotiana benthamiana</em> or <em>D. catenatum</em>, flavonoid content decreased and transcript levels of multiple flavonoid biosynthetic enzyme-coding genes were downregulated. According to these findings, it is proposed that DcMYB30 may negatively regulate flavonoid biosynthesis by down-regulating flavonoid pathway enzyme-coding genes (e.g., 4CL), thereby reducing the flavonoid biosynthesis or accumulation and drought tolerance in <em>D. catenatum</em>. This study provides fundamental insights for characterizing the physiological roles of two-repeat MYB transcription factors in plant stress responses.</div></div>\",\"PeriodicalId\":20234,\"journal\":{\"name\":\"Plant Physiology and Biochemistry\",\"volume\":\"227 \",\"pages\":\"Article 110199\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2025-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology and Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0981942825007272\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942825007272","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
DcMYB30 negatively function in drought tolerance of Dendrobium catenatum by modulating flavonoid biosynthesis
Drought stress is a severe abiotic stress, limiting the plant growth, development and yield. MYB transcription factor family plays a crucial role in plants response to adversity stress, particularly drought. However, a comprehensive analysis of the MYB gene family in Dendrobium catenatum remains limited, especially regarding the functions of two-repeat MYB proteins in response to drought stress and their regulation of flavonoid biosynthesis and accumulation. Here, severe drought stress inhibited the growth of D. catenatum and decreased polysaccharides and flavonoid contents. This study identified 174 MYB genes and characterized their phylogenetic relationships, protein profiles, and expression patterns. Co-expression analysis and transient expression assay revealed that an R2R3-MYB DcMYB30 was a key regulator in drought response and flavonoid synthesis in D. catenatum. DcMYB30 was found to localize in the nucleus and was down-regulated by drought stress. In both DcMYB30-overexpressing Nicotiana benthamiana or D. catenatum, flavonoid content decreased and transcript levels of multiple flavonoid biosynthetic enzyme-coding genes were downregulated. According to these findings, it is proposed that DcMYB30 may negatively regulate flavonoid biosynthesis by down-regulating flavonoid pathway enzyme-coding genes (e.g., 4CL), thereby reducing the flavonoid biosynthesis or accumulation and drought tolerance in D. catenatum. This study provides fundamental insights for characterizing the physiological roles of two-repeat MYB transcription factors in plant stress responses.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.