葡萄（Vitis vinifera L.） CNGC基因全基因组分析及VvCNGC14在拟南芥颖果发育中的功能表征

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-09-17 DOI:10.1016/j.plaphy.2025.110526

Hongsheng Gao , Hongying Wang , BoHui Yu , Xueting Wang , Pan Guo , Shiyang Li , Jieshan Cheng , Chunyan Yu , Hongxia Zhang

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引用次数: 0

摘要

环核苷酸门控通道（CNGCs）在钙摄取中起核心作用。然而，它们在木本植物的生长和对生物胁迫的反应中的准确功能仍不清楚。本研究利用BLAST和HMMER在葡萄基因组中鉴定了22个VvCNGC基因。全基因组关联分析（GWAS）显示，VvCNGC14附近的一个位点与束重相关，并显示出与育性相关的趋势。进一步的表达分析表明，VvCNGC14在花器官，尤其是花粉中有高表达。VvCNGC14在拟南芥cngc18突变体中的异源表达将角果长度、种子大小和种子重量恢复到接近野生型的水平，支持其在角果和种子发育中的作用。系统发育分析表明，VvCNGC14和VvCNGC20因片段重复而亲缘关系密切。蛋白相互作用网络预测鉴定了许多与vvcngc14相互作用的蛋白，包括CDPK家族成员。氧化石墨烯分析预测的蛋白质相互作用突出了钙依赖性蛋白激酶活性、蛋白质自磷酸化、腺苷核糖核苷酸结合和赤霉素3- β -双加氧酶活性的富集。表达分析显示，VvCDPK7、VvCDPK8和VvCDPK16的花粉特异性表达较强，表明VvCNGC14与这些基因之间存在潜在的调控关联。此外，盐和寒冷条件诱导了强大的VvCNGC2/7诱导（qRT-PCR），与应激响应启动子基序一致。总之，这些发现突出了CNGCs在发育和非生物应激反应中的作用。

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Genome-wide analysis of CNGC genes in grapevine (Vitis vinifera L.) and functional characterization of VvCNGC14 in silique development in Arabidopsis

The cyclic nucleotide-gated channels (CNGCs) play a central role in calcium uptake. However, their accurate functions in the growth and response to biological stress in woody plants are still largely unknown. In this study, 22 VvCNGC genes were identified in the grapevine genome using BLAST and HMMER. Genome-wide association study (GWAS) analysis revealed a locus near VvCNGC14 associated with bunch weight and showing a suggestive trend with fertility. Further expression analysis showed that VvCNGC14 was highly expressed in floral organ, particularly pollen. Heterologous expression of VvCNGC14 in Arabidopsis cngc18 mutant restored silique length, seed size, and seed weight to near wild-type levels, supporting a role in silique and seed development. Phylogenetic analysis revealed that VvCNGC14 and VvCNGC20 were closely related due to a segmental duplication event. Protein interaction network prediction identified a number of VvCNGC14-interacting proteins, including CDPK family members. GO analysis of predicted protein interaction highlighted enrichment in calcium-dependent protein kinase activity, protein autophosphorylation, adenyl ribonucleotide binding, and gibberellin 3-beta-dioxygenase activity. Expression analysis indicated strong pollen-specific expression of VvCDPK7, VvCDPK8 and VvCDPK16, suggesting potential regulatory associations between VvCNGC14 and these genes. Furthermore, salt and cold conditions elicited robust VvCNGC2/7 induction (qRT-PCR), aligning with stress-responsive promoter motifs. Together, these findings highlight roles of CNGCs in development and abiotic-stress responses.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.