Plant DiversityPub Date : 2023-09-01DOI: 10.1016/j.pld.2023.02.001
Xien Wu , Dengli Luo , Yingmin Zhang , Ling Jin , M. James C. Crabbe , Qin Qiao , Guodong Li , Ticao Zhang
{"title":"Integrative analysis of the metabolome and transcriptome reveals the potential mechanism of fruit flavor formation in wild hawthorn (Crataegus chungtienensis)","authors":"Xien Wu , Dengli Luo , Yingmin Zhang , Ling Jin , M. James C. Crabbe , Qin Qiao , Guodong Li , Ticao Zhang","doi":"10.1016/j.pld.2023.02.001","DOIUrl":"https://doi.org/10.1016/j.pld.2023.02.001","url":null,"abstract":"<div><p>Hawthorns are important medicinal and edible plants with a long history of health protection in China. Besides cultivated hawthorn, other wild hawthorns may also have excellent medicinal and edible value, such as <em>Crataegus</em> <em>chungtienensis</em>, an endemic species distributed in the Southwest of China. In this study, by integrating the flavor-related metabolome and transcriptome data of the ripening fruit of <em>C. chungtienensis</em>, we have developed an understanding of the formation of hawthorn fruit quality. The results show that a total of 849 metabolites were detected in the young and mature fruit of <em>C. chungtienensis</em>, of which flavonoids were the most detected metabolites. Among the differentially accumulated metabolites, stachyose, maltotetraose and cis-aconitic acid were significantly increased during fruit ripening, and these may be important metabolites affecting fruit flavor change. Moreover, several flavonoids and terpenoids were reduced after fruit ripening compared with young fruit. Therefore, using the unripe fruit of <em>C. chungtienensis</em> may allow us to obtain more medicinal active ingredients such as flavonoids and terpenoids. Furthermore, we screened out some differentially expressed genes (DEGs) related to fruit quality formation, which had important relationships with differentially accumulated sugars, acids, flavonoids and terpenoids. Our study provides new insights into flavor formation in wild hawthorn during fruit development and ripening, and at the same time this study lays the foundation for the improvement of hawthorn fruit flavor.</p></div>","PeriodicalId":20224,"journal":{"name":"Plant Diversity","volume":"45 5","pages":"Pages 590-600"},"PeriodicalIF":4.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49810903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant DiversityPub Date : 2023-09-01DOI: 10.1016/j.pld.2023.03.012
Hai-Su Hu , Jiu-Yang Mao , Xue Wang , Yu-Ze Liang , Bei Jiang , De-Quan Zhang
{"title":"Plastid phylogenomics and species discrimination in the “Chinese” clade of Roscoea (Zingiberaceae)","authors":"Hai-Su Hu , Jiu-Yang Mao , Xue Wang , Yu-Ze Liang , Bei Jiang , De-Quan Zhang","doi":"10.1016/j.pld.2023.03.012","DOIUrl":"https://doi.org/10.1016/j.pld.2023.03.012","url":null,"abstract":"<div><p><em>Roscoea</em> is an alpine or subalpine genus from the pan-tropical family Zingiberaceae, which consists of two disjunct groups in geography, namely the “Chinese” clade and the “Himalayan” clade. Despite extensive research on the genus, <em>Roscoea</em> species remain poorly defined and relationships between these species are not well resolved. In this study, we used plastid genomes of nine species and one variety to resolve phylogenetic relationships within the “Chinese” clade of <em>Roscoea</em> and as DNA super barcodes for species discrimination. We found that <em>Roscoea</em> plastid genomes ranged in length from 163,063 to 163,796 bp, and encoded 113 genes, including 79 protein-coding genes, 30 tRNA genes, four rRNA genes. In addition, expansion and contraction of the IR regions showed obvious infraspecific conservatism and interspecific differentiation. Plastid phylogenomics revealed that species belonging to the “Chinese” clade of <em>Roscoea</em> can be divided into four distinct subclades. Furthermore, our analysis supported the independence of <em>R</em>. <em>cautleoides</em> var. <em>pubescens</em>, the recovery of <em>Roscoea pubescens</em> Z.Y. Zhu, and a close relationship between <em>R</em>. <em>humeana</em> and <em>R</em>. <em>cautloides</em>. When we used the plastid genome as a super barcode, we found that it possessed strong discriminatory power (90%) with high support values. Intergenic regions provided similar resolution, which was much better than that of protein-coding regions, hypervariable regions, and DNA universal barcodes. However, plastid genomes could not completely resolve <em>Roscoea</em> phylogeny or definitively discriminate species. These limitations are likely related to the complex history of <em>Roscoea</em> speciation, poorly defined species within the genus, and the maternal inheritance of plastid genomes.</p></div>","PeriodicalId":20224,"journal":{"name":"Plant Diversity","volume":"45 5","pages":"Pages 523-534"},"PeriodicalIF":4.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49810897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant DiversityPub Date : 2023-09-01DOI: 10.1016/j.pld.2022.12.008
Yi Jin , Hong Qian
{"title":"Drivers of the differentiation between broad-leaved trees and shrubs in the shift from evergreen to deciduous leaf habit in forests of eastern Asian subtropics","authors":"Yi Jin , Hong Qian","doi":"10.1016/j.pld.2022.12.008","DOIUrl":"https://doi.org/10.1016/j.pld.2022.12.008","url":null,"abstract":"<div><p>In eastern Asian subtropical forests, leaf habit shifts from evergreen to deciduous broad-leaved woody plants toward higher latitudes. This shift has been largely explained by the greater capacity of deciduous broad-leaved plants to respond to harsh climatic conditions (e.g., greater seasonality). The advantages of deciduous leaf habit over evergreen leaf habit in more seasonal climates have led us to hypothesize that leaf habits would shift in response to climate changes more conspicuously in forest canopy trees than in forest understory shrubs. Furthermore, we hypothesize that in the forests of the subtropics, plants at higher latitudes, regardless of growth form, would better tolerate seasonal harsh climates, and hence show less differentiation in leaf habit shift, compared to those at lower latitudes. To test these two hypotheses, we modelled the proportion of deciduous broad-leaved species and the incidence of deciduous and evergreen broad-leaved species in woody angiosperm species compositions of ten large-sized forest plots distributed in the Chinese subtropics. We found that the rate of leaf habit shift along a latitudinal gradient was higher in forest trees than in forest shrubs. We also found that the differentiation in leaf habit shift between trees and shrubs is greater at lower latitudes (i.e., warmer climates) than at higher latitudes (i.e., colder climates). These findings indicate that specialized forest plants are differentially affected by climate in distinct forest strata in a manner dependent on latitudinal distribution. These differences in forest plant response to changes in climate suggest that global climate warming will alter growth forms and geographical distributions and ranges of forests.</p></div>","PeriodicalId":20224,"journal":{"name":"Plant Diversity","volume":"45 5","pages":"Pages 535-543"},"PeriodicalIF":4.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49810901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant DiversityPub Date : 2023-07-01DOI: 10.1016/j.pld.2022.11.007
Korina Ocampo-Zuleta , Ángela Parrado-Rosselli
{"title":"Functional diversity in an Andean subpáramo affected by wildfire in Colombia","authors":"Korina Ocampo-Zuleta , Ángela Parrado-Rosselli","doi":"10.1016/j.pld.2022.11.007","DOIUrl":"10.1016/j.pld.2022.11.007","url":null,"abstract":"<div><p>Recently, the Andean subpáramo in Colombia has experienced severe wildfires, but little is known about the functional composition of recovering or not after a wildfire. Therefore, we examined the functional community composition subpáramo affected by fire in 2016. We documented how functional traits changed 31 months after the disturbance and compared them with an unburned site. We sampled from one to two years after the fire every four months, then registered all recruits in 16 5 × 5m plots. New individuals were classified into strategy functional groups based on the traits of persistence and dispersal. The first group was stem type and regeneration mechanism (seedling and resprout), and the second was fruit type and dispersal mode. We investigated the degree to which functional diversity changes plant communities over time (woody and non-woody), and we compared it with an unburned site. The most relevant results showed that resprouts and seed regenerated increased post-fire time and significant differences between sampling periods. The anemochory is the most relevant dispersal mode that indicates the community capacity to colonize the new gaps opened by the fire rapidly. We discuss how wildfire appears to be a triggering factor for persistence and dispersal strategy groups in subpáramo burned given their characteristics of tolerance to stress. For this reason, a greater functional divergence between the ecosystems studied post-fire recovery has been related to higher levels of biodiversity at the landscape scale due to the high degree of endemism and significant differences in species composition between páramos.</p></div>","PeriodicalId":20224,"journal":{"name":"Plant Diversity","volume":"45 4","pages":"Pages 385-396"},"PeriodicalIF":4.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7e/9a/main.PMC10436060.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10052160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant DiversityPub Date : 2023-07-01DOI: 10.1016/j.pld.2023.03.004
Xue-Min Xu , Dan-Hui Liu , Shi-Xin Zhu , Zhen-Long Wang , Zhen Wei , Quan-Ru Liu
{"title":"Phylogeny of Trigonotis in China—with a special reference to its nutlet morphology and plastid genome","authors":"Xue-Min Xu , Dan-Hui Liu , Shi-Xin Zhu , Zhen-Long Wang , Zhen Wei , Quan-Ru Liu","doi":"10.1016/j.pld.2023.03.004","DOIUrl":"https://doi.org/10.1016/j.pld.2023.03.004","url":null,"abstract":"<div><p>The genus <em>Trigonotis</em> comprises nearly 60 species mainly distributed in East and Southeast Asia. China has the largest number of <em>Trigonotis</em> species in the world, with a total of 44 species, of which 38 are endemic. Nutlet morphology is useful for the taxonomic delimitation of <em>Trigonotis</em>. However, there are still controversial circumscriptions of nutlet shape in some species. In previous studies, interspecies phylogenetic relationships were inferred using few DNA markers and very few taxa, which possibly led to erroneous or incomplete conclusions. In this study, the nutlet morphology of 39 <em>Trigonotis</em> taxa and the characteristics of 34 complete chloroplast genomes (29 taxa) were investigated and analyzed. Then, the phylogenetic relationships were discussed within this genus based on complete chloroplast genomes. To the best of our knowledge, this study is the first comprehensive analysis of nutlet morphology and complete chloroplast genome of <em>Trigonotis</em>. Based on nutlet morphology, <em>Trigonotis</em> can be divided into two groups: Group 1, hemispherical or oblique tetrahedron with carpopodiums, and Group 2, inverted tetrahedron without carpopodiums<em>.</em> The chloroplast genome of <em>Trigonotis</em> exhibited a typical quadripartite structure, including 84–86 protein-coding, 37 transfer RNA, and 8 ribosomal RNA genes, with a total length of 147,247–148,986 bp. Genes in the junctions were well conserved in <em>Trigonotis</em>, similar to those in other Boraginaceae s.str. species. Furthermore, <em>Trigonotis</em> chloroplast genomes showed relatively high diversity, with more conserved genic regions than intergenic regions; in addition, we detected 14 hot spots (Pi > 0.005) in non-coding regions. Phylogenetic analyses based on chloroplast genome data identified highly resolved relationships between <em>Trigonotis</em> species. Specifically, <em>Trigonotis</em> was divided into two clades with strong support: one clade included species with hemispherical or oblique tetrahedron nutlets with carpopodiums and bracts, whereas the other clade included species with inverted tetrahedron nutlets without carpopodiums or bracts. Our results may inform future taxonomic, phylogenetic, and evolutionary studies on Boraginaceae.</p></div>","PeriodicalId":20224,"journal":{"name":"Plant Diversity","volume":"45 4","pages":"Pages 409-421"},"PeriodicalIF":4.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49894973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant DiversityPub Date : 2023-07-01DOI: 10.1016/j.pld.2022.09.007
Gang Feng , Ying-Jie Xiong , Hua-Yu Wei , Yao Li , Ling-Feng Mao
{"title":"Endemic medicinal plant distribution correlated with stable climate, precipitation, and cultural diversity","authors":"Gang Feng , Ying-Jie Xiong , Hua-Yu Wei , Yao Li , Ling-Feng Mao","doi":"10.1016/j.pld.2022.09.007","DOIUrl":"10.1016/j.pld.2022.09.007","url":null,"abstract":"<div><p>Medicinal plants provide crucial ecosystem services, especially in developing countries such as China, which harbors diverse endemic medicinal plant species with substantial cultural and economic value. Accordingly, understanding the patterns and drivers of medicinal plant distribution is critical. However, few studies have investigated the patterns and drivers of endemic medicinal plants distribution in China. Here, we linked endemic medicinal plants distribution with possible explanatory variables, i.e., paleoclimate change, contemporary climate, altitudinal range and ethnic minority human population size at the prefecture city level in China. Our results show that endemic medicinal plants are concentrated in southern China, especially in southwestern China. Notably, both endemic medicinal plant species richness and the ratio of endemic medicinal plant species richness are negatively associated with glacial-interglacial anomaly in temperature, and positively associated with contemporary precipitation and altitudinal range. In addition, we found that endemic medicinal plant species richness is positively associated with ethnic minority population sizes as well as its ratio to the overall population size. These findings suggest that the distribution of endemic medicinal plants is determined by multiple drivers. Furthermore, our findings stress that dramatic future climate changes and massive anthropogenic activities in southern China pose great challenges to the conservation of China's endemic medicinal plants.</p></div>","PeriodicalId":20224,"journal":{"name":"Plant Diversity","volume":"45 4","pages":"Pages 479-484"},"PeriodicalIF":4.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/72/48/main.PMC10435908.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10050577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant DiversityPub Date : 2023-07-01DOI: 10.1016/j.pld.2022.10.005
Cindy Q. Tang , Shi-Qian Yao , Peng-Bin Han , Jian-Ran Wen , Shuaifeng Li , Ming-Chun Peng , Chong-Yun Wang , Tetsuya Matsui , Yong-Ping Li , Shan Lu , Yuan He
{"title":"Forest characteristics, population structure and growth trends of threatened relict Pseudotsuga forrestii in China","authors":"Cindy Q. Tang , Shi-Qian Yao , Peng-Bin Han , Jian-Ran Wen , Shuaifeng Li , Ming-Chun Peng , Chong-Yun Wang , Tetsuya Matsui , Yong-Ping Li , Shan Lu , Yuan He","doi":"10.1016/j.pld.2022.10.005","DOIUrl":"https://doi.org/10.1016/j.pld.2022.10.005","url":null,"abstract":"<div><p><em>Pseudotsuga forrestii</em> is a relict evergreen coniferous tree species in Pinaceae endemic to China. <em>P. forrestii</em> tree numbers have greatly decreased due to deforestation, over-utilization and habitat degradation. Here we clarify <em>P. forrestii</em> community types, structure, species diversity, seedling recruitments and growth trends. We identified four <em>P. forrestii</em> community types: (1) <em>Pseudotsuga forrestii - Quercus guyavifolia - A</em><em>cer davidii</em> evergreen coniferous and broad-leaved mixed forest; (2) <em>Pseudotsuga forrestii - P</em><em>inus yunnanensis - Quercus guyavifolia</em> evergreen coniferous and broad-leaved mixed forest; (3) <em>Pseudotsuga forrestii</em> evergreen coniferous forest; (4) <em>Pseudotsuga forrestii - A</em><em>bies georgei</em> var. <em>smithii</em> evergreen coniferous forest. <em>P. forrestii</em> forests are characterized by both warm temperate and temperate affinities. Simpson diversity, Pielou evenness, Shannon–Wiener diversity indices ranged from 0.75 to 0.76, 0.74–0.81, and 1.62–1.93, respectively, with no significant differences among the four forest types. The forest stratification was multilayered. The canopy layer was generally 10–25 m tall, with the emergent layer reached 25–42 m. DBH and age structures of <em>P. forrestii</em> showed multimodal distributions. Its maximum age <em>P. forrestii</em> was 570 years with a DBH of 143 cm. The growth of annual ring width of <em>P. forrestii</em> was slow, and generally decreased with age, whereas the basal area at the breast height increased with age. Established seedlings/saplings were mainly found in unstable micro-habitats. Regeneration of <em>P. forrestii</em> depends on moderate natural disturbances. Finally, we provide recommendations for <em>P. forrestii</em> conservation.</p></div>","PeriodicalId":20224,"journal":{"name":"Plant Diversity","volume":"45 4","pages":"Pages 422-433"},"PeriodicalIF":4.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49894974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant DiversityPub Date : 2023-07-01DOI: 10.1016/j.pld.2023.03.013
Na Su , Richard G.J. Hodel , Xi Wang , Jun-Ru Wang , Si-Yu Xie , Chao-Xia Gui , Ling Zhang , Zhao-Yang Chang , Liang Zhao , Daniel Potter , Jun Wen
{"title":"Molecular phylogeny and inflorescence evolution of Prunus (Rosaceae) based on RAD-seq and genome skimming analyses","authors":"Na Su , Richard G.J. Hodel , Xi Wang , Jun-Ru Wang , Si-Yu Xie , Chao-Xia Gui , Ling Zhang , Zhao-Yang Chang , Liang Zhao , Daniel Potter , Jun Wen","doi":"10.1016/j.pld.2023.03.013","DOIUrl":"https://doi.org/10.1016/j.pld.2023.03.013","url":null,"abstract":"<div><p><em>Prunus</em> is an economically important genus widely distributed in the temperate Northern Hemisphere. Previous studies on the genus using a variety of loci yielded conflicting phylogenetic hypotheses. Here, we generated nuclear reduced representation sequencing data and plastid genomes for 36 <em>Prunus</em> individuals and two outgroups. Both nuclear and plastome data recovered a well-resolved phylogeny<em>.</em> The species were divided into three main clades corresponding to their inflorescence types, - the racemose group, the solitary-flower group and the corymbose group - with the latter two sister to one another. <em>Prunus</em> was inferred to have diversified initially in the Late Cretaceous around 67.32 million years ago. The diversification of the three major clades began between the Paleocene and Miocene, suggesting that paleoclimatic events were an important driving force for <em>Prunus</em> diversification. Ancestral state reconstructions revealed that the most recent common ancestor of <em>Prunus</em> had racemose inflorescences, and the solitary-flower and corymb inflorescence types were derived by reduction of flower number and suppression of the rachis, respectively. We also tested the hybrid origin hypothesis of the racemose group proposed in previous studies. <em>Prunus</em> has undergone extensive hybridization events, although it is difficult to identify conclusively specific instances of hybridization when using SNP data, especially deep in the phylogeny. Our study provides well-resolved nuclear and plastid phylogenies of <em>Prunus</em>, reveals substantial cytonuclear discord at shallow scales, and sheds new light on inflorescence evolution in this economically important lineage.</p></div>","PeriodicalId":20224,"journal":{"name":"Plant Diversity","volume":"45 4","pages":"Pages 397-408"},"PeriodicalIF":4.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49796437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plant DiversityPub Date : 2023-07-01DOI: 10.1016/j.pld.2022.10.001
Ting-Ting Zou, Sen-Tao Lyu, Qi-Lin Jiang, Shu-He Shang, Xiao-Fan Wang
{"title":"Pre- and post-pollination barriers between two exotic and five native Sagittaria species: Implications for species conservation","authors":"Ting-Ting Zou, Sen-Tao Lyu, Qi-Lin Jiang, Shu-He Shang, Xiao-Fan Wang","doi":"10.1016/j.pld.2022.10.001","DOIUrl":"https://doi.org/10.1016/j.pld.2022.10.001","url":null,"abstract":"<div><p>Anthropogenic introduction of species has resulted in a breakdown of geographical barriers and hybridization in previously allopatric species. Thus, examining hybridization proneness of exotic species contributes to revealing its potential threat. Moreover, reproductive barriers may be strengthened or weakened due to long-term geographical isolation for these newly sympatric species. However, few studies have evaluated multiple barriers between alien and native species. In this study, we quantified the importance of four pre-pollination barriers (phenological, floral traits, pollen production, and floral constancy) and four post-pollination barriers (pollen-pistil incompatibility, seed set, seed viability, and seedling survival) between two introduced and five native <em>Sagittaria</em> species. Results showed that introduced <em>S</em>. <em>platyphylla</em> was cross-compatible with two native species, whereas introduced <em>S</em>. <em>montevidensis</em> was incapable of hybridizing with any native species. Different barriers were asymmetric within species pairs and multiple barriers acted in concert to maintain species boundaries. Post-pollination barriers contributed more to total reproductive isolation in native species, whereas pre-pollination barriers played a stronger role in total reproductive isolation for two introduced species. Seed set was the only barrier that was positively correlated with genetic distance. Our results provide a perspective to better understand reproductive barriers for secondary contact species. We highlight the importance of monitoring hybridization events before human introduction and the possible conservation strategies to remove invasive species with hybridization proneness.</p></div>","PeriodicalId":20224,"journal":{"name":"Plant Diversity","volume":"45 4","pages":"Pages 456-468"},"PeriodicalIF":4.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49796435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}