Plant Physiology最新文献_第4页

Shrunken2 (Sh2) and Brittle2 (Bt2) play a crucial role in the developmental processes of the aleurone layers in maize. 枯缩2 （Sh2）和脆性2 （Bt2）在玉米糊粉层发育过程中起着至关重要的作用。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf464

Yimo Du,Boming Yang,Yipeng Lu,Li Zhao,Tengfei Zhang,Jie Liu,Yuanrong Pei,Darun Cai,Huairen Zhang,Zhiliang Zhang,Huiyong Li,Lifeng Wang,Long Zhang,Huabang Chen,Juan Liu

{"title":"Shrunken2 (Sh2) and Brittle2 (Bt2) play a crucial role in the developmental processes of the aleurone layers in maize.","authors":"Yimo Du,Boming Yang,Yipeng Lu,Li Zhao,Tengfei Zhang,Jie Liu,Yuanrong Pei,Darun Cai,Huairen Zhang,Zhiliang Zhang,Huiyong Li,Lifeng Wang,Long Zhang,Huabang Chen,Juan Liu","doi":"10.1093/plphys/kiaf464","DOIUrl":"https://doi.org/10.1093/plphys/kiaf464","url":null,"abstract":"The aleurone layer, located on the outermost region of the endosperm, is rich in lipids, proteins, and minerals and can substantially enhance grain nutritional value with increased thickness. ADP-glucose pyrophosphorylase is a critical rate-limiting enzyme in starch biosynthesis, with Shrunken2 (Sh2) and Brittle2 (Bt2) encoding its large and small subunits, respectively. Nevertheless, the influence of these genes on maize (Zea mays) aleurone layer development remains poorly understood. We conducted genetic analysis, map-based cloning, and allelic validation using the Huajian1F (HJ1F) paternal line containing multi-aleurone layers, and identified that Sh2 regulates aleurone layer thickness in HJ1F. Additional studies demonstrated that the bt2 mutant displayed the phenotype of aleurone layer thickening. Both sh2 and bt2 mutants exhibited a substantial increase in the contents of grain oil, total protein, and mineral elements. RNA sequencing analysis indicated that differentially expressed genes were enriched in fatty acid and amino acid metabolic pathways during aleurone layer formation. ZmDof3 (DNA-binding one zinc finger protein 3) and NKD2 (NAKED ENDOSPERM2), which participate in aleurone layer thickening in maize, showed significant differential expression during the aleurone layer development of sh2 and bt2 mutants. Yeast one-hybrid assays, β-galactosidase activity assays, and the luciferase reporter system confirmed that ZmDof3 and NKD2 regulate the expression of Sh2 and Bt2. This study has established that Sh2 and Bt2 control starch biosynthesis and play a pivotal role in maize aleurone layer development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"7 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The exocyst subunit EXO70D2 interacts with the WD40 protein GORI to regulate pollen tube growth and seed setting in rice. 外囊亚基EXO70D2与WD40蛋白GORI相互作用，调控水稻花粉管生长和结实率。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf422

Ranran Tu, Hong Wang, Qinwen Zou, Zhihao Sun, Jiajun Wu, Guanghua He, Nan Wang

引用次数: 0

The natural Msc-4 allele confers genic male sterility via conserved and divergent gene regulatory mechanisms in pepper. 天然的Msc-4等位基因通过保守和分化的基因调控机制赋予辣椒基因雄性不育性。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf474

Heshan Du,Xiaofei Zhang,Ren Li,Bin Chen,Xiaofen Zhang,Yihao Wang,Jinfang Wang,Ting Wang,Changlong Wen,Ning Liu,Sansheng Geng

{"title":"The natural Msc-4 allele confers genic male sterility via conserved and divergent gene regulatory mechanisms in pepper.","authors":"Heshan Du,Xiaofei Zhang,Ren Li,Bin Chen,Xiaofen Zhang,Yihao Wang,Jinfang Wang,Ting Wang,Changlong Wen,Ning Liu,Sansheng Geng","doi":"10.1093/plphys/kiaf474","DOIUrl":"https://doi.org/10.1093/plphys/kiaf474","url":null,"abstract":"Utilization of genic male sterility (GMS) significantly reduces production costs and ensures the high varietal purity of hybrid seeds, thereby demonstrating considerable potential for hybrid seed production in pepper (Capsicum annuum). In this study, we report the characterization of a pepper GMS mutant, male sterility from China-4 (msc-4), which exhibits aborted pollen grains without detectable exine. Strikingly, the vegetative growth of these pepper plants was unaffected. Genome-based mapping and functional analysis of msc-4 revealed that a nonsense mutation in a cytochrome P450 family gene, CYP703A2/Msc-4, results in pollen development failure and subsequent GMS. Msc-4 transcripts specifically accumulated in the tapetum and microspores of developing anthers. Comparative transcriptome analysis suggested that fatty acid and sporopollenin metabolism are severely impaired in msc-4 mutants. Furthermore, we demonstrated that the AMS-MYB80-CYP703A2/Msc-4 regulatory cascade is generally conserved, albeit with some differences, in pepper. Importantly, mutations in the tomato and cucumber CYP703A2 orthologs conferred male sterility without growth penalty, strongly supporting that CYP703A2 genes are ideal targets for the generation of GMS germplasms. Overall, our findings shed light on the molecular basis of Msc-4 in pepper, demonstrating that mutagenesis of CYP703A2 genes can be readily adapted for a broad range of vegetable crops.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"93 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H3 acetylation triggers SWR1 recruitment to promote H2A.Z incorporation into chromatin and stimulate gene expression. H3乙酰化触发SWR1募集，促进H2A。与染色质结合并刺激基因表达。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf469

Han Jiang,Mingming Zhao,Min Liu,Wenshuo Zhang,Junjie Xu,Thierry Pélissier,Olivier Mathieu,Israel Ausin,Yafei Wang

引用次数: 0

The MdVQ17/MdVQ37-MdWRKY100 module coordinates apple salt tolerance by modulating Na+/K+ homeostasis and ROS scavenging. MdVQ17/MdVQ37-MdWRKY100模块通过调节Na+/K+稳态和ROS清除来协调苹果的耐盐性。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf434

Dingyue Duan,Ran Yi,Mingrui Shi,Jiwei Zhou,Qinglong Dong,Ke Mao,Jie Yang,Fengwang Ma

{"title":"The MdVQ17/MdVQ37-MdWRKY100 module coordinates apple salt tolerance by modulating Na+/K+ homeostasis and ROS scavenging.","authors":"Dingyue Duan,Ran Yi,Mingrui Shi,Jiwei Zhou,Qinglong Dong,Ke Mao,Jie Yang,Fengwang Ma","doi":"10.1093/plphys/kiaf434","DOIUrl":"https://doi.org/10.1093/plphys/kiaf434","url":null,"abstract":"Salt stress greatly affects the yield and quality of agricultural crops, limiting their geographic distribution. Valine-glutamine (VQ) motif-containing proteins function as transcriptional regulators of plant growth and stress responses. However, the role and underlying molecular mechanisms of VQ proteins in apple (Malus domestica) salt stress responses remain largely unknown. Here, MdVQ17 was identified as a salt-responsive gene that negatively regulates salt tolerance in apple plants by increasing the Na+/K+ ratio and suppressing reactive oxygen species (ROS) scavenging under salt stress. MdWRKY100, a WRKY transcription factor that enhances apple salt tolerance, was identified as an MdVQ17-interacting protein. MdWRKY100 directly bound to the Arabidopsis K⁺ TRANSPORTER 1-LIKe (MdAKT1-like) and PEROXIDASE 57 (MdPER57) promoters, activating their transcription. MdVQ17 inhibited the binding and transcriptional activation activity of MdWRKY100 at these promoters, thereby suppressing MdWRKY100-mediated regulation of Na+/K+ homeostasis and antioxidant enzyme activity. MdVQ37, another MdWRKY100-interacting VQ protein, modulated the MdWRKY100-mediated salt response through a similar mechanism. Overall, these findings reveal that the MdVQ17/MdVQ37-MdWRKY100 module co-regulates Na+/K+ homeostasis and ROS scavenging by modulating MdAKT1-like and MdPER57 expression, providing insights into how the VQ-WRKY complex influences salt tolerance in apple.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"17 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allelic variants of IRKI contribute to photosynthetic efficiency by regulating rubisco activase in Populus IRKI等位变异通过调控rubisco激活酶对杨树光合效率的影响

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf465

Yongsen Jiang, Dan Wang, Linxia Yang, Yu Cheng, Luying Yang, Tingxuan Zhao, Donghai Zhang, Jiaxuan Zhou, Zitian Li, Yicen Guan, Tailin Ren, Yuling He, Qingzhang Du, Deqiang Zhang, Mingyang Quan

{"title":"Allelic variants of IRKI contribute to photosynthetic efficiency by regulating rubisco activase in Populus","authors":"Yongsen Jiang, Dan Wang, Linxia Yang, Yu Cheng, Luying Yang, Tingxuan Zhao, Donghai Zhang, Jiaxuan Zhou, Zitian Li, Yicen Guan, Tailin Ren, Yuling He, Qingzhang Du, Deqiang Zhang, Mingyang Quan","doi":"10.1093/plphys/kiaf465","DOIUrl":"https://doi.org/10.1093/plphys/kiaf465","url":null,"abstract":"Photosynthesis directly determines plant biomass accumulation by controlling carbon flow and energy input. Thus, increasing photosynthetic efficiency is a promising approach for boosting plant growth and yield. However, the genetic basis of photosynthesis in perennial woody plants remains largely unknown, and the causative alleles warrant comprehensive investigation. Here, we performed a genome-wide association study (GWAS) on photosynthetic traits in a natural population of Chinese white poplar (Populus tomentosa). We identified inflorescence and root apices receptor-like kinase-interacting protein (IRKI) as a causative gene of photosynthesis that is significantly associated with the activity of rubisco activase (Rca). The seventh leaves of PtoIRKI-OE plants exhibited a 27.77% increase in net photosynthetic rate (Pn), a 31.42% rise in starch content, and a 16.83% expansion in leaf area compared to wild-type plants, whereas ptoirki-KD plants displayed opposite phenotypes. Further analyses indicated that PtoIRKI interacted with PtoRca to enhance Rca activity, leading to increases in the activation state of ribulose bisphosphate carboxylase oxygenase (rubisco) and photosynthetic efficiency. Importantly, we identified an elite haplotype, PtoIRKIhap2, which exhibited higher PtoIRKI expression and Pn than PtoIRKIhap1. Finally, we found that homeodomain-leucine zipper protein 1 (PtoHB1) specifically bound to the PtoIRKIhap2 promoter, thereby promoting PtoIRKI expression and photosynthetic efficiency, as validated by integrating machine learning models and molecular experiments. Our results shed light on the molecular mechanism through which PtoIRKI modulates photosynthetic efficiency. We also provide an excellent haplotype module, PtoHB1-PtoIRKIhap2-PtoRca, that can be used to improve the photosynthesis of woody plants via molecular breeding.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"114 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and transcriptomic basis of heterosis in three intra- and intersubspecific hybrid rice 三个亚种内和亚种间杂交稻杂种优势的遗传和转录组学基础

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf427

Jian Che, Tao Yang, Haichuan Yang, Guangwei Li, Zhengji Wang, Qinglu Zhang, Xu Li, Yidan Ouyang

{"title":"Genetic and transcriptomic basis of heterosis in three intra- and intersubspecific hybrid rice","authors":"Jian Che, Tao Yang, Haichuan Yang, Guangwei Li, Zhengji Wang, Qinglu Zhang, Xu Li, Yidan Ouyang","doi":"10.1093/plphys/kiaf427","DOIUrl":"https://doi.org/10.1093/plphys/kiaf427","url":null,"abstract":"Heterosis has been crucial for global food security, particularly in hybrid rice (Oryza sativa L.) development. While indica-japonica intersubspecific hybrids offer a 20-30% yield increase over indica-indica hybrids, the mechanisms underlying intersubspecific heterosis remain poorly explored. Here, we sequenced and phenotyped three pairwise crosses and their derived populations from representative japonica and indica varieties. Intersubspecific hybrids exhibited stronger heterosis and superior biomass than intrasubspecific hybrids. Transcriptomic analysis demonstrated that brassinosteroid signaling and flowering-promoting genes are up-regulated in intrasubspecific hybrids to accelerate growth, while intersubspecific hybrids display enhanced tillering capacity and yield potential through elevated transcriptional activity and gene expression related to nitrogen use efficiency. Genome-wide allele-specific expression analysis highlighted the regulatory impact of parental genomic divergence on hybrid gene expression, particularly through promoter variations affecting genes involved in flowering, tillering, pollen fertility, and stress response. QTL mapping revealed a greater number of loci associated with heterosis in intersubspecific hybrids than in intrasubspecific hybrids, particularly for spikelets per panicle and plant height, where the Nipponbare allele and indica alleles function as advantageous alleles across distinct QTLs; their synergistic interactions collectively drive the heterosis between subspecies. Our study reveals that parental genetic diversity combined with dominant expression patterns collectively drives heterosis, providing valuable insights for optimizing intersubspecific hybrid rice breeding to enhance yield and agricultural sustainability.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"17 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Under control: a WRKY transcription factor positively regulates the expression of a helper NLR immune receptor in N. benthamiana. 在控制下：WRKY转录因子正向调节benthamiana中辅助NLR免疫受体的表达。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf445

Josephine H R Maidment

引用次数: 0

Arabidopsis lipid kinase FAB1 regulates lateral root emergence via clathrin-mediated endocytosis of PIN-FORMED 3. 拟南芥脂质激酶FAB1通过网格蛋白介导的PIN-FORMED 3内吞作用调控侧根出苗。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf459

Jiaqi Ma,Jianxin Shou,Danlu Han,Mei Xu,Yuting Yao,Liufan Wang,Yuling Ran,Haijun Wu,Chengwei Yang,Jun Wang,Sebastian Bednarek,Xu Yan,Chao Wang

{"title":"Arabidopsis lipid kinase FAB1 regulates lateral root emergence via clathrin-mediated endocytosis of PIN-FORMED 3.","authors":"Jiaqi Ma,Jianxin Shou,Danlu Han,Mei Xu,Yuting Yao,Liufan Wang,Yuling Ran,Haijun Wu,Chengwei Yang,Jun Wang,Sebastian Bednarek,Xu Yan,Chao Wang","doi":"10.1093/plphys/kiaf459","DOIUrl":"https://doi.org/10.1093/plphys/kiaf459","url":null,"abstract":"Lateral root (LR) growth enables plants to explore regions of the soil, anchor themselves, and optimize nutrient and water uptake. LR development is characterized by the establishment of an auxin maximum at the lateral root primordium (LRP); however, the regulatory mechanisms underlying this process remain poorly understood. In Arabidopsis (Arabidopsis thaliana), FORMATION OF APLOID AND BINUCLEATE CELLS1 (FAB1) encodes a lipid kinase responsible for biosynthesizing phosphatidylinositol 3,5-bisphosphate. Our findings show that auxin induces FAB1 expression through AUXIN RESPONSE FACTOR7/19 (ARF7/19). In turn, FAB1 modulates the auxin maximum at the LRP tip by regulating clathrin-mediated endocytosis of the PIN-FORMED (PIN) auxin efflux carriers, forming a previously uncharacterized auxin-induced feedback loop during LR development. Loss of FAB1 function markedly increases LR number, while FAB1 overexpression suppresses LR formation. Diminished FAB1 activity leads to less clathrin accumulation at the plasma membrane (PM), limited endocytosis, and greater PIN3 retention at the LRP PM, thereby enhancing auxin flow toward LRP tips and promoting emergence. These results identify FAB1 as a negative regulator of lateral root development that fine-tunes auxin transport to facilitate organ emergence.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"91 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Further evidence supporting the existence and accumulation of triacylglycerol and acylplastoquinol in cyanobacteria. 进一步的证据支持三酰基甘油和酰基塑喹啉在蓝藻细菌的存在和积累。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf478

Naoki Sato,Haruhiko Jimbo,Toru Yoshitomi,Tsubasa Shoji,Mayuko Sato,Noriko Takeda,Kiminori Toyooka,Hajime Wada

{"title":"Further evidence supporting the existence and accumulation of triacylglycerol and acylplastoquinol in cyanobacteria.","authors":"Naoki Sato,Haruhiko Jimbo,Toru Yoshitomi,Tsubasa Shoji,Mayuko Sato,Noriko Takeda,Kiminori Toyooka,Hajime Wada","doi":"10.1093/plphys/kiaf478","DOIUrl":"https://doi.org/10.1093/plphys/kiaf478","url":null,"abstract":"Triacylglycerol (TAG) has been frequently reported in cyanobacteria; however, unlike in plants and algae, the isolation of TAG as a pure substance and its subsequent chemical characterization have proven challenging. The slr2103 gene in Synechocystis sp. PCC 6803, which encodes a putative acyltransferase involved in TAG biosynthesis, has been considered evidence for the existence of TAG. However, the identification of acylplastoquinol (APQ) as the major component of the \"TAG fraction\" obtained through thin-layer chromatography (TLC) has raised questions about the actual presence of TAG in cyanobacteria. To address concerns regarding potential chemical and biological contamination in the detection of TAG in cyanobacteria, we developed 1D- and 2D-TLC methods to separate submicromole quantities of TAG and APQ from Synechocystis cells. Both compounds were convincingly identified using NMR and LC/MS. TAG levels depended upon culture conditions. Well-aerated cyanobacterial cultures exhibited minimal TAG levels, while TAG accumulation reached approximately 1% of total lipids in static or slowly swirled senescing cultures, where photosynthetic activity had declined substantially. Under these conditions, we observed numerous lipid globules, approximately 72 nm in diameter, located at the periphery of the cells. These findings provide critical insights into TAG and APQ accumulation in cyanobacteria, elucidating the role of lipid globules and offering perspectives on TAG biosynthesis in cyanobacteria, as well as the potential function of APQ in photosynthesis.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"4 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0