Plant Physiology最新文献_第4页

Virulence effectors encoded in the rice yellow dwarf phytoplasma genome participate in pathogenesis 水稻黄矮病病原菌基因组编码的病毒效应子参与致病过程

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-07 DOI: 10.1093/plphys/kiae601

Shuai Zhang, Peng Gan1, Huiting Xie, Chuan Li, Tianxin Tang, Qiong Hu, Zhihong Zhu, Zhongkai Zhang, Jisen Zhang, Yongsheng Zhu, Qun Hu, Jie Hu, Hongxin Guan, Shanshan Zhao, Jianguo Wu

引用次数: 0

The potato sugar transporter SWEET1g affects apoplasmic sugar ratio and phloem-mobile tuber- and flower-inducing signals 马铃薯糖转运体 SWEET1g 影响细胞质糖比率以及块茎和花的韧皮部移动诱导信号

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-07 DOI: 10.1093/plphys/kiae602

Angelique Lauschke, Leonie Maibaum, Mira Engel, Luise Eisengräber, Sina Beyer, Aleksandra Hackel, Christina Kühn

{"title":"The potato sugar transporter SWEET1g affects apoplasmic sugar ratio and phloem-mobile tuber- and flower-inducing signals","authors":"Angelique Lauschke, Leonie Maibaum, Mira Engel, Luise Eisengräber, Sina Beyer, Aleksandra Hackel, Christina Kühn","doi":"10.1093/plphys/kiae602","DOIUrl":"https://doi.org/10.1093/plphys/kiae602","url":null,"abstract":"The main phloem loader in potato, sucrose transporter StSUT1, is co-expressed with two members of the SWEET gene family: StSWEET11b, a clade III member of SWEET carriers assumed to be involved in sucrose efflux, and StSWEET1g, a clade I member involved in glucose efflux into the apoplast that physically interacts with StSUT1. We investigated the functionality of SWEET carriers via uptake experiments with fluorescent glucose or sucrose analogs. Inhibition or overexpression of StSWEET1g/SlSWEET1e affected tuberization and flowering in transgenic potato plants. Isolation of the apoplasmic fluid by vacuum infiltration centrifugation revealed changes in the apoplasmic hexose composition and mono-to-disaccharide ratio, affecting sink strength. Down-regulation of StSWEET1g expression affected the expression of SP6A, a tuberigen, and miR172 under LD conditions, leading to early flowering and tuberization. A systematic screen for StSWEET1g-interacting protein partners revealed several proteins affecting cell wall integrity and strengthening. StSWEET1g and the main interaction partners were strongly down-regulated during tuber development. We discuss whether StSWEET1g activity might be linked to cell wall remodeling during tuber development and the switch from apoplasmic to symplasmic phloem unloading.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"42 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factor PagWRKY33 regulates gibberellin signaling and immune receptor pathways in Populus. 转录因子 PagWRKY33 调节杨树赤霉素信号和免疫受体通路。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-06 DOI: 10.1093/plphys/kiae593

Xiao-Qian Yu, Hao-Qiang Niu, Yue-Mei Zhang, Xiao-Xu Shan, Chao Liu, Hou-Ling Wang, Weilun Yin, Xinli Xia

{"title":"Transcription factor PagWRKY33 regulates gibberellin signaling and immune receptor pathways in Populus.","authors":"Xiao-Qian Yu, Hao-Qiang Niu, Yue-Mei Zhang, Xiao-Xu Shan, Chao Liu, Hou-Ling Wang, Weilun Yin, Xinli Xia","doi":"10.1093/plphys/kiae593","DOIUrl":"10.1093/plphys/kiae593","url":null,"abstract":"Enhanced autoimmunity often leads to impaired plant growth and development, and the coordination of immunity and growth in Populus remains elusive. In this study, we have identified the transcription factors PagWRKY33a/b as key regulators of immune response and growth maintenance in Populus. The disruption of PagWRKY33a/b causes growth issues and autoimmunity while conferring resistance to anthracnose caused by Colletotrichum gloeosporioides. PagWRKY33a/b binds to the promoters of N requirement gene 1.1 (NRG1.1) and Gibberellic Acid-Stimulated in Arabidopsis (GASA14)during infection, activating their transcription. This process maintains disease resistance and engages in GA signaling to reduce growth costs from immune activation. The oxPagWRKY33a/nrg1.1 mutant results in reduced resistance to C. gloeosporioides. Further, PagWRKY33a/b is phosphorylated and activated by Mitogen-Activated Protein Kinase Kinase 1 (MKK1), which inhibits Respiratory Burst Oxidase Homolog D (RBOHD) and Respiratory Burst Oxidase Homolog I (RBOHI) transcription, causing ROS bursts in wrky33a/b double mutants. This leads to an upregulation of PagNRG1.1 in the absence of pathogens. However, the wrky33a/b/nrg1.1 and wrky33a/b/rbohd triple mutants show compromised defense responses, underscoring the complexity of WRKY33 regulation. Additionally, the stability of PagWRKY33 is modulated by Ring Finger Protein 5 (PagRNF5)-mediated ubiquitination, balancing plant immunity and growth. Together, our results provide key insights into the complex function of WRKY33 in Populus autoimmunity and its impact on growth and development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MYB168 and WRKY20 transcription factors synergistically regulate lignin monomer synthesis during potato tuber wound healing. MYB168 和 WRKY20 转录因子协同调控马铃薯块茎伤口愈合过程中木质素单体的合成。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-05 DOI: 10.1093/plphys/kiae573

Ruirui Yang, Qihui Wang, Ying Wang, Xuejiao Zhang, Xiaoyuan Zheng, Yongcai Li, Dov Prusky, Yang Bi, Ye Han

{"title":"MYB168 and WRKY20 transcription factors synergistically regulate lignin monomer synthesis during potato tuber wound healing.","authors":"Ruirui Yang, Qihui Wang, Ying Wang, Xuejiao Zhang, Xiaoyuan Zheng, Yongcai Li, Dov Prusky, Yang Bi, Ye Han","doi":"10.1093/plphys/kiae573","DOIUrl":"https://doi.org/10.1093/plphys/kiae573","url":null,"abstract":"Lignin is a critical component of the closing layer of the potato (Solanum tuberosum L.) tuber during healing; however, the molecular mechanism of its formation remains poorly understood. To elucidate the molecular mechanism of tuber healing, we screened the genes encoding transcription factors that regulate lignin synthesis(StMYB24/49/105/144/168, StWRKY19/20/22/23/34) and the key genes involved in lignin monomer synthesis (PHENYLALANINE AMMONIA LYASE 5 (StPAL5) and CINNAMYL ALCOHOL DEHYDROGENASE 14 (StCAD14)) for induced expression after wounding using transcriptome data. DLR, Y1H, EMSA, and ChIP-qPCR assays revealed that StMYB168 could bind directly to the StPAL5 and StCAD14 promoters to activate their expression and that StWRKY20 enhanced this regulation with a synergistic effect. Y2H, BiFC, and Co-IP assays showed that StMYB168 interacted with StWRKY20 to form a MYB-WRKY complex. Furthermore, transient overexpression of StMYB168 and StWRKY20 in Nicotiana benthamiana leaves upregulated the expression of NbPAL and NbCAD10 and promoted lignin accumulation in the leaves. In addition, overexpression of StWRKY20 and StMYB168 together resulted in higher expression levels of NbPAL and NbCAD10 and higher levels of lignin monomer and total lignin. In contrast, silencing of StMYB168 and StWRKY20 in potato significantly reduced the lignin content of wounded tubers. In conclusion, StMYB168 and StWRKY20 are important regulators of lignin biosynthesis in potato tubers during healing and can positively regulate lignin biosynthesis by forming a complex. The elucidation of this regulatory module provides information on the regulatory mechanism of lignin monomer synthesis in wounded tubers at the transcriptional level.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Flux Analysis to Increase Oil in Seeds. 通过代谢通量分析提高种子含油量

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-05 DOI: 10.1093/plphys/kiae595

Thiya Mukherjee, Shrikaar Kambhampati, Stewart A Morley, Timothy P Durrett, Doug K Allen

{"title":"Metabolic Flux Analysis to Increase Oil in Seeds.","authors":"Thiya Mukherjee, Shrikaar Kambhampati, Stewart A Morley, Timothy P Durrett, Doug K Allen","doi":"10.1093/plphys/kiae595","DOIUrl":"https://doi.org/10.1093/plphys/kiae595","url":null,"abstract":"Ensuring an adequate food supply and enough energy to sustainably support future global populations will require enhanced productivity from plants. Oilseeds can help address these needs; but the fatty acid composition of seed oils is not always optimal, and higher yields are required to meet growing demands. Quantitative approaches including metabolic flux analysis can provide insights on unexpected metabolism (i.e., when metabolism is different than in a textbook) and can be used to guide engineering efforts; however, as metabolism is context-specific, it changes with tissue type, local environment, and development. This review describes recent insights from metabolic flux analysis in oilseeds and indicates engineering opportunities based on emerging topics and developing technologies that will aid quantitative understanding of metabolism and enable efforts to produce more oil. We also suggest that investigating the key regulators of fatty acid biosynthesis, such as transcription factors, and exploring metabolic signals like phytohormones in greater depth through flux analysis, could open new pathways for advancing genetic engineering and breeding strategies to enhance oil crop production.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osmotic stress in roots drives lipoxygenase-dependent plastid remodeling through singlet oxygen production. 根中的渗透胁迫通过产生单线态氧驱动脂氧合酶依赖性质体重塑

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-05 DOI: 10.1093/plphys/kiae589

Dekel Cohen-Hoch, Tomer Chen, Lior Sharabi, Nili Dezorella, Maxim Itkin, Gil Feiguelman, Sergey Malitsky, Robert Fluhr

{"title":"Osmotic stress in roots drives lipoxygenase-dependent plastid remodeling through singlet oxygen production.","authors":"Dekel Cohen-Hoch, Tomer Chen, Lior Sharabi, Nili Dezorella, Maxim Itkin, Gil Feiguelman, Sergey Malitsky, Robert Fluhr","doi":"10.1093/plphys/kiae589","DOIUrl":"https://doi.org/10.1093/plphys/kiae589","url":null,"abstract":"Osmotic stress, caused by the lack of water or by high salinity, is a common problem in plant roots. Osmotic stress can be reproducibly simulated with the application of solutions of the high-molecular-weight and impermeable polyethylene glycol. The accumulation of different reactive oxygen species, such as singlet oxygen, superoxide, and hydrogen peroxide, accompany this stress. Among them, singlet oxygen, produced as a byproduct of lipoxygenase activity, has been associated with limiting root growth. To better understand the source and effect of singlet oxygen, we followed its production at the cellular level in Arabidopsis (Arabidopsis thaliana). Osmotic stress initiated profound changes in plastid and vacuole structure. Confocal and electron microscopy showed that the plastids were a source of singlet oxygen accompanied by the appearance of multiple, small extraplastidic bodies that were also an intense source of singlet oxygen. A marker protein, CRUMPLED LEAF, indicated that these small bodies originated from the plastid outer membrane. Remarkably, LINOLEATE 9S-LIPOXYGENASE 5, (LOX5), was shown to change its distribution from uniformly cytoplasmic to a more clumped distribution together with plastids and the small bodies. In addition, oxylipin products of type 9 lipoxygenase increased, while products of type 13 lipoxygenases decreased. Inhibition of lipoxygenase by the SHAM inhibitor or in down-regulated lipoxygenase lines prevented cells from initiating the cellular responses, leading to cell death. In contrast, singlet oxygen scavenging halted terminal cell death. These findings underscore the reversible nature of osmotic stress-induced changes, emphasizing the pivotal roles of lipoxygenases and singlet oxygen in root stress physiology.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutation of GLR2 confers enhanced glufosinate resistance and salt tolerance in rice. GLR2 基因突变增强了水稻的草铵膦抗性和耐盐性。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-05 DOI: 10.1093/plphys/kiae588

Weimin Cheng, Yan Ren, Jiayi Wang, Chunpeng Chen, Cheng Fang, Lingling Peng, Dongyang Zhang, Liangzhi Tao, Yue Zhan, Kun Wu, Yuejin Wu, Binmei Liu, Xiangdong Fu, Yafeng Ye

引用次数: 0

Epigenetic control of T-DNA during transgenesis and pathogenesis. 转基因和致病过程中 T-DNA 的表观遗传控制。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-05 DOI: 10.1093/plphys/kiae583

Joaquin Felipe Roca Paixao, Angélique Déléris

引用次数: 0

AlGrow: a graphical interface for easy, fast and accurate area and growth analysis of heterogeneously colored targets. AlGrow：图形界面，可对异色目标进行简便、快速、准确的面积和生长分析。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-05 DOI: 10.1093/plphys/kiae577

Marcus McHale, Ronan Sulpice

引用次数: 0

Epigenetic factors direct synergistic and antagonistic regulation of transposable elements in Arabidopsis. 表观遗传因子对拟南芥中转座元素的协同和拮抗调控起指导作用。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-04 DOI: 10.1093/plphys/kiae392

Jo-Wei Allison Hsieh, Ming-Ren Yen, Fu-Yu Hung, Keqiang Wu, Pao-Yang Chen

{"title":"Epigenetic factors direct synergistic and antagonistic regulation of transposable elements in Arabidopsis.","authors":"Jo-Wei Allison Hsieh, Ming-Ren Yen, Fu-Yu Hung, Keqiang Wu, Pao-Yang Chen","doi":"10.1093/plphys/kiae392","DOIUrl":"10.1093/plphys/kiae392","url":null,"abstract":"Arabidopsis (Arabidopsis thaliana) HISTONE DEACETYLASE 6 (HDA6) and HISTONE DEMETHYLASES LSD-LIKE 1 (LDL1) and LDL2 synergistically regulate the expression of long non-coding RNAs associated with H3Ac and H3K4me2. The underlying mechanisms of such highly coordinated interactions among genetic and epigenetic factors contributing to this collaborative regulation remain largely unclear. We analyzed all transposable elements (TEs) across the Arabidopsis genome and the individual and combined roles of HDA6 and LDL1/LDL2 by dissecting multilayered epigenomes and their association with transcription. Instead of an individual synergistic effect, we observed dual synergistic and antagonistic effects, which are positively associated with H3Ac and H3K4me2 while maintaining a negative but moderate association with DNA methylation. Specifically, 2 modes of synergistic regulation were discovered in TEs: 74% are primarily regulated by HDA6, with less dependence on LDL1/LDL2, and the remaining 26% are co-regulated by both. Between the 2 modes, we showed that HDA6 has a strong effect on TE silencing, whereas LDL1/LDL2 plays a weaker yet crucial role in co-regulation with HDA6. Our results led to a model of epigenomic regulation-the differential de-repression between the 2 modes of synergistic regulation of TEs was determined by H3Ac and H3K4me2 levels, where TEs are in accessible chromatins free of DNA methylation, and this open chromatin environment precedes transcriptional changes and epigenome patterning. Our results discovered unbalanced effects of genetic factors in synergistic regulation through delicately coordinated multilayered epigenomes and chromatin accessibility.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"1939-1952"},"PeriodicalIF":6.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11531835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0