Plant Physiology最新文献_第4页

Ontologically spiky: Behind trichome formation in lilies sepals.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-01 DOI: 10.1093/plphys/kiaf063

Sebastián R Moreno

引用次数: 0

Systemic evaluation of the inhibitory effects of 4 anti-CRISPR systems on different Cas12a nucleases in rice.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-01 DOI: 10.1093/plphys/kiaf097

Yao He, Shishi Liu, Xuelian Zheng, Yiping Qi, Yong Zhang

引用次数: 0

Potato tuberization under long-day conditions: Genetic effects of 6 CYCLING DOF FACTOR1 alleles.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-01 DOI: 10.1093/plphys/kiaf098

Lianlian Ma, Haicai Li, Hao Jiang, Yanhui Zhu, Zhong Zhang, Dawei Li, Guangtao Zhu, Qijun Sui, Yinqiao Jian, Jianjian Qi, Zefeng Zhai, Chunzhi Zhang

引用次数: 0

Viral delivery of recombinases activates heritable genetic switches in plants.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-01 Epub Date: 2025-02-21 DOI: 10.1093/plphys/kiaf073

James C Chamness, Jon P Cody, Anna J Cruz, Daniel F Voytas

{"title":"Viral delivery of recombinases activates heritable genetic switches in plants.","authors":"James C Chamness, Jon P Cody, Anna J Cruz, Daniel F Voytas","doi":"10.1093/plphys/kiaf073","DOIUrl":"10.1093/plphys/kiaf073","url":null,"abstract":"Viral vectors provide an increasingly versatile platform for transformation-free reagent delivery to plants. RNA viral vectors can be used to induce gene silencing, overexpress proteins, or introduce gene editing reagents; however, they are often constrained by carrying capacity or restricted tropism in germline cells. Site-specific recombinases that catalyze precise genetic rearrangements are powerful tools for genome engineering that vary in size and, potentially, efficacy in plants. In this work, we show that viral vectors based on tobacco rattle virus (TRV) deliver and stably express four recombinases ranging in size from ∼0.6 to ∼1.5 kb and achieve simultaneous marker removal and reporter activation through targeted excision in transgenic Nicotiana benthamiana lines. TRV vectors with Cre, FLP, CinH, and Integrase13 efficiently mediated recombination in infected somatic tissue and led to heritable modifications at high frequency. An excision-activated Ruby reporter enabled simple and high-resolution tracing of infected cell lineages without the need for molecular genotyping. Together, our experiments broaden the scope of viral recombinase delivery and offer insights into infection dynamics that may be useful in developing future viral vectors.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"197 3","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial DNA and the largest nuclear-mitochondrial DNA in Arabidopsis can be separated by their methylation levels.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-01 DOI: 10.1093/plphys/kiaf069

Yuyang Zhong, Miki Okuno, Nobuhiro Tsutsumi, Shin-Ichi Arimura

{"title":"Mitochondrial DNA and the largest nuclear-mitochondrial DNA in Arabidopsis can be separated by their methylation levels.","authors":"Yuyang Zhong, Miki Okuno, Nobuhiro Tsutsumi, Shin-Ichi Arimura","doi":"10.1093/plphys/kiaf069","DOIUrl":"10.1093/plphys/kiaf069","url":null,"abstract":"Methylation of cytosines in plant mitochondrial DNA (mtDNA) has been a controversial issue. Results supporting mtDNA methylation may have been subject to contamination due to the presence of nuclear sequences originating from the mitochondrial genome called nuclear mitochondrial insertions (NUMT). In Arabidopsis (Arabidopsis thaliana) Columbia 0 (Col-0), the largest NUMT, located on Chromosome 2, is nearly twice the size of the entire mitochondrial genome and exhibits a sequence almost identical to the mitochondrial genome, albeit with shuffling and repeats. In the presence of such high similarity, it is challenging to eliminate interference when determining mtDNA methylation levels. Here, we applied a methyl-CpG-binding domain (MBD) protein-based affinity assay to separate total DNA, applied next-generation sequencing to the pre- and postseparation DNA samples, and examined the single nucleotide polymorphism (SNP) sites between NUMT and mtDNA. The results revealed successful separation of methylated and non-methylated DNA within the total DNA, with simultaneous separation achieved between NUMT DNA and mtDNA. These results suggest that our method can achieve separation based on the differential methylation levels of the whole lengths of NUMT and mtDNAs. The bisulfite sequencing results for the postseparation DNA samples suggest that mtDNA exhibits not only a lack of CpG methylation but also an absence of CHH and CHG methylation. In contrast, the NUMT shows high levels of methylation across all 3 contexts, at least in the Col-0 accession.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The tomato SBP-box protein SlCNR negatively regulates genes involved in fruit cutin deposition.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-01 DOI: 10.1093/plphys/kiaf099

Di Chen, Hua Huang, Qiaoli Zhang, Tingting Hu, Zongyan Sun, Tingyu Wang, Xue Chen, Rongrong Nie, Leilei Zhou, Daqi Fu, Hongliang Zhu, Guozheng Qin, Benzhong Zhu, Guiqin Qu

{"title":"The tomato SBP-box protein SlCNR negatively regulates genes involved in fruit cutin deposition.","authors":"Di Chen, Hua Huang, Qiaoli Zhang, Tingting Hu, Zongyan Sun, Tingyu Wang, Xue Chen, Rongrong Nie, Leilei Zhou, Daqi Fu, Hongliang Zhu, Guozheng Qin, Benzhong Zhu, Guiqin Qu","doi":"10.1093/plphys/kiaf099","DOIUrl":"10.1093/plphys/kiaf099","url":null,"abstract":"Cutin serves as the foundational structure of the plant cuticle and plays a crucial role in determining fruit development and quality. However, the transcriptional regulation of cutin deposition by fruit-specific transcription factors remains largely unknown. This study delves into the regulatory role of the tomato (Solanum lycopersicum) SBP-box protein Colorless Non-Ripening (SlCNR), primarily expressed in fruits, in cutin formation. We found that clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9-induced slcnr mutants exhibited thicker cuticles and elevated contents in total cutin and cutin monomers compared with wild-type fruits, whereas SlCNR overexpression (OE) lines displayed the opposite tendency. Transcriptome-wide RNA sequencing identified differentially expressed genes in SlCNR OE fruits. Further validation by gene expression, DNA binding, and transcriptional activity assays revealed that SlCNR directly binds to and represses the transcription of 13 genes associated with cutin synthesis, export, and assembly, including glycerol-3-phosphate acyltransferase 4/6, ATP-binding cassette transporter subfamily G protein 36/42, and cutin synthase. In addition, SlCNR directly bound to and repressed the transcription activities of the promoter of NON-RIPENING like-1, which encodes a positive regulator of cutin deposition. Thus, SlCNR emerged as a negative transcription regulator of cutin content and cuticle thickness, ultimately affecting fruit firmness and cuticle permeability. This study sheds light on the molecular mechanisms governing cutin deposition through transcriptional regulatory networks, highlighting the role of SlCNR as an important player in fruit cuticle development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resistance gene enrichment sequencing refines the Brassica napus NLRome. 利用 RenSeq 完善甘蓝菜 NLRome。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-01 DOI: 10.1093/plphys/kiae631

Jiaxu Wu 吴家旭, Soham Mukhopadhyay, Edel Pérez-López

引用次数: 0

Oiling the wheels of growth: TOR kinase promotes lipid synthesis in plants.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-01 DOI: 10.1093/plphys/kiaf065

Moona Rahikainen

引用次数: 0

GT1 and ZmHB13/VRL1 regulate flower sexual differentiation by modulating jasmonate biosynthesis and signaling in maize.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-01 DOI: 10.1093/plphys/kiaf075

Yateng Yuan, Xiaotong Ou, Minhao Yao, Pinle Wang, Chunlian Li, Guisen Zhang, Mengqi He, Heying Li, Xin Xu, Zhuojun Zhong, Yun Lu, Xiaojun Lai, Chen Zou, Jie Shen, Tianyu Zhang, Defan Liu, Yaoyao Li, Haiyang Wang, Dexin Kong, Qing Liu

{"title":"GT1 and ZmHB13/VRL1 regulate flower sexual differentiation by modulating jasmonate biosynthesis and signaling in maize.","authors":"Yateng Yuan, Xiaotong Ou, Minhao Yao, Pinle Wang, Chunlian Li, Guisen Zhang, Mengqi He, Heying Li, Xin Xu, Zhuojun Zhong, Yun Lu, Xiaojun Lai, Chen Zou, Jie Shen, Tianyu Zhang, Defan Liu, Yaoyao Li, Haiyang Wang, Dexin Kong, Qing Liu","doi":"10.1093/plphys/kiaf075","DOIUrl":"10.1093/plphys/kiaf075","url":null,"abstract":"As a typical monoecious plant, maize (Zea mays L.) produces unisexual male and female flowers comprising tassels and ears, respectively. However, the molecular mechanisms underlying unisexual flower development remain largely unclear. Here, we identify a pair of homeodomain-leucine zipper (HD-ZIP) transcription factors, Grassy tiller1 (GT1) and ZmHB13 (also termed VRS1-like1, VRL1), which act synergistically to control carpel development in maize. Mutations in GT1, but not ZmHB13/VRL1, cause defects in carpel abortion in the tassels, resulting in feminized tassels. The gt1 Zmhb13 double mutants show a notably more severe phenotype and a defect in the abortion of the lower floret in the ear spikelet, resulting in extra fertile flowers and seed setting. We demonstrate that GT1 and ZmHB13/VRL1 bind directly to the promoters of 2 jasmonate (JA) biosynthesis genes, ZmLOX3 and ZmOPR7, and upregulate their expression in the developing flowers. The application of methyl jasmonate (MeJA) rescues the feminized tassel phenotype of the gt1 Zmhb13 double mutants. Additionally, GT1 and ZmHB13/VRL1 interact with the key JA-pathway repressors ZmJAZ4 and ZmJAZ26, thus releasing the key JA-pathway activators ZmMYC2a and ZmMYC2b to activate the downstream JA signaling processes. Our findings indicate that GT1 and ZmHB13/VRL1 function synergistically to regulate maize carpel development through modulating JA biosynthesis and signaling.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The TRAPPC8/TRS85 subunit of the Arabidopsis TRAPPIII tethering complex regulates endoplasmic reticulum function and autophagy.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-01 DOI: 10.1093/plphys/kiaf042

Marta Hoffman-Sommer, Natalia Piłka, Anna Anielska-Mazur, Julita Nowakowska, Małgorzata Kozieradzka-Kiszkurno, Cezary Pączkowski, Małgorzata Jemioła-Rzemińska, Kamil Steczkiewicz, Yasin Dagdas, Ewa Swiezewska

{"title":"The TRAPPC8/TRS85 subunit of the Arabidopsis TRAPPIII tethering complex regulates endoplasmic reticulum function and autophagy.","authors":"Marta Hoffman-Sommer, Natalia Piłka, Anna Anielska-Mazur, Julita Nowakowska, Małgorzata Kozieradzka-Kiszkurno, Cezary Pączkowski, Małgorzata Jemioła-Rzemińska, Kamil Steczkiewicz, Yasin Dagdas, Ewa Swiezewska","doi":"10.1093/plphys/kiaf042","DOIUrl":"10.1093/plphys/kiaf042","url":null,"abstract":"Transport protein particle (TRAPP) tethering complexes are known for their function as Rab GTPase exchange factors. Two versions of the complex are considered functionally separate: TRAPPII, an activator of the Rab11 family (RabA in plants) GTPases that function in post-Golgi sorting, and TRAPPIII, activating Rab1 family (RabD in plants) members that regulate endoplasmic reticulum (ER)-to-Golgi trafficking and autophagy. In Arabidopsis (Arabidopsis thaliana), the TRAPPIII complex has been identified and its subunit composition established, but little is known about its functions. Here, we found that binary subunit interactions of the plant TRAPPIII complex are analogous to those of metazoan TRAPPIII, with the 2 large subunits TRAPPC8 and TRAPPC11 linking the TRAPP core and the small C12 to C13 dimer. To gain insight into the functions of TRAPPIII in plants, we characterized 2 A. thaliana trappc8 mutants. These mutants display abnormalities in plant morphology, particularly in flower and seed development. They also exhibit autophagic defects, a constitutive ER stress response, and elevated levels of the ER lipid dolichol (Dol), which is an indispensable cofactor in protein glycosylation. These results indicate that plant TRAPPC8 is involved in multiple cellular trafficking events and suggest a link between ER stress responses and Dol levels.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"197 3","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0