Plant Physiology最新文献

{"title":"CRISPR-based Transcriptional activation deciphers strigolactone biosynthesis in\u0000 Nicotiana benthamiana","authors":"Juan Pablo Arciniegas,Derry Alvarez,Jian You Wang,Marta Vazquez-Vilar,Diego Orzaez,Salim Al-Babili","doi":"10.1093/plphys/kiag748","DOIUrl":"https://doi.org/10.1093/plphys/kiag748","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"53 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR editing of TERMINAL FLOWER 1 recapitulates perpetual flowering in strawberry, mimicking repression by the PERPETUAL FLOWERING locus.","authors":"Marta Bjornson, Michael A Hardigan, Juan M Debernardi, Hillel Brukental, Dominique D A Pincot, Mishi V Vachev, Randi A Famula, Glenn S Cole, Dianne Velasco, Maher Al Rwahnih, Mitchell J Feldmann, Steven J Knapp","doi":"10.1093/plphys/kiag684","DOIUrl":"10.1093/plphys/kiag684","url":null,"abstract":"<p><p>The development of perpetual flowering (PF) cultivars revolutionized octoploid strawberry (Fragaria  ×  ananassa) cultivation by enabling nearly year-round fruit production and doubling fruit yields compared to historic seasonal flowering (SF) cultivars, which flower in spring. Most commercial PF cultivars result from the introgression of a dominant PERPETUAL FLOWERING (PF) allele at the PERPETUAL FLOWERING RUNNERING (PFRU) locus. PFRU has been genetically and physically mapped to chromosome 4B, but the causal gene(s) remain unknown. Here we used the haplotype-phased \"UCD Royal Royce\" (PFpf) genome, next-generation sequencing-based k-mer genome-wide association study (k-mer GWAS), and RNAseq to investigate PFRU. We narrowed the locus down to a precise physical address and revealed abundant genetic and transcriptomic differences in the locus and associated with flowering. Outside the PFRU locus, flowering regulation diverges at expression of TERMINAL FLOWER 1 (TFL1) in all 4 subgenomes, analogous to diploid Fragaria vesca. This critical step of the flowering pathway is altered by PF in diverse cultivars, and gene editing knockout of TFL1 is sufficient to induce flowering under long days in a seasonal flowering cultivar. Together, these results shed light on the mechanism of flowering regulation by the PF locus and expand biotechnological approaches to achieve extended flowering in any strawberry cultivar.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"202 2","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13650783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149137561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering transgenic alfalfa with improved biomass and enhanced heat-humidity tolerance.","authors":"Chao Yang, Jing Li, Yuping Li, Dingyun Cen, Yamei Ma, Ying Wang","doi":"10.1093/plphys/kiag754","DOIUrl":"10.1093/plphys/kiag754","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The loss of COP1 delays senescence and inhibits root growth through modulation of sucrose allocation and availability.","authors":"Seung Ju Lee, Wang Ki Min, Jong Tae Song, Hak Soo Seo","doi":"10.1093/plphys/kiag704","DOIUrl":"10.1093/plphys/kiag704","url":null,"abstract":"<p><p>CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) participates in diverse signaling pathways that regulate plant growth and development, but its role in sucrose-mediated signaling remains unclear. Here, we show that COP1 contributes to leaf senescence and root growth by modulating sucrose allocation and availability in Arabidopsis. Under sucrose-free conditions, cop1 mutants developed chlorotic leaves with reduced chlorophyll content, impaired primary root growth, and decreased starch granule formation in root columella cells. cop1 mutants also showed reduced expression of sucrose transport- and catabolism-related genes in leaves, suggesting impaired source-to-sink sugar allocation and reduced sucrose turnover in source tissues. Consistent with this possibility, exogenous glucose and sucrose supplementation alleviated leaf chlorosis and increased chlorophyll content and primary root growth, while sucrose supplementation also partially restored starch granule formation in root columella cells. Moreover, overexpression of SUCROSE-PROTON SYMPORTER 2, a key sucrose transporter for shoot-to-root sucrose transport that was downregulated in cop1 mutants, partially restored chlorophyll content, primary root growth, and starch granule formation. COP1 loss was also accompanied by reduced brassinosteroid (BR) signaling, including decreased expression of BR-related genes, reduced BRASSINAZOLE-RESISTANT 1 (BZR1) accumulation, attenuated brassinolide (BL) responsiveness, and decreased endogenous BL content, along with decreased expression of ethylene biosynthesis- and senescence-associated genes and delayed leaf senescence. Together, these findings indicate that loss of COP1 disrupts sucrose allocation and availability, with sucrose accumulating in leaves while the delivery of shoot-derived sucrose to roots is limited, thereby impairing starch granule formation and root growth and reducing BR/ethylene-associated senescence responses, ultimately delaying leaf senescence.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13650782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VmUGT88A65 and VmUGT88A66 orchestrate root development and pathogen resistance in tung tree via flavonoid and lignin glycosylation.","authors":"Yang Yang, Jia Wang, Fuchuan Han, Ming Gao, Yunxiao Zhao, Yicun Chen, Yangdong Wang","doi":"10.1093/plphys/kiag491","DOIUrl":"10.1093/plphys/kiag491","url":null,"abstract":"<p><p>Vernicia montana possesses a robust root system and exhibits high resistance to the soil-borne fungus Fusarium. The molecular mechanism underlying this resistance remains largely elusive. Here, we identified the species-specific UDP-glucosyltransferase coding genes VmUGT88A65 and VmUGT88A66 in V. montana through evolutionary analysis. These genes displayed exclusively root-specific expression patterns and were significantly upregulated upon infection with Fof-1. Transgenic tung trees overexpressing VmUGT88A65 and VmUGT88A66 exhibited a substantial increase in root index from 1.59 g to 2.16 g and enhanced resistance to Fof-1, which was associated with elevated levels of flavonoid and lignin glycosides. Conversely, RNAi-mediated suppression of VmUGT88A65 and VmUGT88A66 resulted in increased susceptibility to Fof-1, reduced root size, and decreased glycoside accumulation. In vitro and in vivo experiments further confirmed that VmUGT88A65 and VmUGT88A66 catalyze the glycosylation of specific compounds, producing flavonoid glycosides (N7G and Q7G) and lignin glycosides (CA4G and FA4G). Exogenous application of a combination of N7G and Q7G significantly inhibited Fof-1 mycelial growth and host infection in vitro. Additionally, CA4G and FA4G contributed to enhanced lignin deposition in roots, thereby strengthening the physical barrier against Fof-1. Our findings reveal a pathway through which VmUGT88A65 and VmUGT88A66 overexpression enhances the accumulation of flavonoid and lignin glycosides and promotes root growth, collectively contributing to improved disease resistance in tung trees.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A tail of 2 proteins: the cytoplasmic tail of Cf receptor-like proteins modulates effector-triggered activation of cell death.","authors":"Josephine H R Maidment","doi":"10.1093/plphys/kiag632","DOIUrl":"https://doi.org/10.1093/plphys/kiag632","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"202 2","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13660677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From smoke to symbiosis: dissecting KAI2 signaling in rice using the specific receptor agonist, dMGer.","authors":"James M Bradley","doi":"10.1093/plphys/kiag389","DOIUrl":"10.1093/plphys/kiag389","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13640472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148265660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Derepression of BRANCHED1-controlled sucrose hydrolysis drives bud outgrowth and phytoplasma-induced symptoms in Ziziphus jujuba.","authors":"Fuli Ma, Lei Zhong, Yu Liu, Zhenyuan Zhang, Yunyan Zheng, Jia Si, Mingsheng Deng, Junyong Zhou, Qibao Sun, Jun Sun","doi":"10.1093/plphys/kiag702","DOIUrl":"10.1093/plphys/kiag702","url":null,"abstract":"<p><p>Sugar metabolism and signaling play major roles in the early events of axillary bud outgrowth by repressing BRANCHED 1 (BRC1) expression. However, whether BRC1 controls sugar metabolism during the cessation of axillary bud growth remains unknown. In previous research, it was shown that jujube witches' broom (JWB) phytoplasma effectors SJP1 and SJP2-mediated ZjBRC1 degradation induced lateral main bud (LMB) outgrowth in Ziziphus jujuba. Here, we found that derepression of ZjBRC1-suppressed sucrose hydrolysis drove JWB phytoplasma-induced LMB outgrowth. ZjBRC1 complementation repressed axillary bud outgrowth in the Arabidopsis brc1-2 mutant by increasing sucrose accumulation. ZjBRC1 directly bound to and downregulated the promoter activities of the vacuolar invertase ZjVIN2, the neural or alkaline invertase ZjCIN9, and the sucrose synthase ZjSUSY5, contributing to sucrose accumulation. Fusing the SRDX repression domain to the C terminus of ZjBRC1 (ZjBRC1-SRDX) counteracted the repression and promoted sucrose hydrolysis in jujube calli. Compared with the control, JWB phytoplasma infection reduced the sucrose content and ZjBRC1 accumulation in growing LMBs, which was consistent with the results of the decapitation treatment. The effector SJP2 disrupted the balance between sucrose homeostasis and growth cessation, resulting in a shortened and repeated bud break-active growth cycle of LMBs in transgenic jujube plants. In this study, we propose a potential model for JWB phytoplasma-mediated LMB outgrowth in which ZjBRC1 regulates sugar metabolism and signaling in a negative feedback loop in Z. jujuba.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149002363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is resistance ever free? Weighing the costs of altered strigolactone biosynthesis in sorghum.","authors":"Neeta Lohani, Rita A Sharma","doi":"10.1093/plphys/kiag692","DOIUrl":"10.1093/plphys/kiag692","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13650780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The old nourishes the new: 100 years of leaf senescence studies in Plant Physiology.","authors":"Pablo Ignacio Calzadilla, Judy Brusslan","doi":"10.1093/plphys/kiag697","DOIUrl":"10.1093/plphys/kiag697","url":null,"abstract":"<p><p>The remarkable efficiency of plants is exemplified by leaf senescence, in which older leaves slowly perish while catabolizing and exporting the nutrients that made them prolific photosynthetic factories. Several endogenous (eg ontogeny and phenology) and exogenous (eg environmental stressors) factors can induce this physiological process. These multiple pathways, together with their complex regulatory networks, make senescence challenging to understand. The coordination between organelles, cells, and organs, across multiple levels of regulation (eg, genetic, metabolic, and physiological), further complicates the identification of the signals that initiate this process and the associated transduction pathways. Nevertheless, unraveling this complexity has remained a significant goal for plant biologists. The critical process of leaf senescence has been studied in Plant Physiology for 100 years. Research has focused on 6 main questions: (i) Which hormones regulate leaf senescence? (ii) How is highly abundant Rubisco catabolized? (iii) How do reproductive sinks regulate leaf senescence? (iv) How does photosynthesis change during leaf senescence? (v) What role do reactive oxygen species (ROS) play in leaf senescence? and (vi) How does gene expression change during leaf senescence? This review focuses on Plant Physiology's long-standing track record of publishing the foundational, field-defining work that has shaped core conceptual advances in leaf senescence research and provides context for future studies that will continue to address how leaves slowly and productively end their lives. Many other contributing papers have been cited in this review; however, our coverage is incomplete. We suggest readers view additional reviews for a more complete understanding of leaf senescence (Woo HR, Kim HJ, Lim PO, Nam HG. 2019. Leaf senescence: systems and dynamics aspects. Annu Rev Plant Biol. 70:347-376; Guo Y et al. 2021. Leaf senescence: progression, regulation, and application. Mol Hortic. 1:5; Zhang Y-M, Guo P, Xia X, Guo H, Li Z. 2021. Multiple layers of regulation on leaf senescence: new advances and perspectives. Front Plant Sci. 12:788996; Cao J, Liu H, Tan S, Li Z. 2023. Transcription factors-regulated leaf senescence: current knowledge, challenges and approaches. Int J Mol Sci. 24:9245; Zhai Y, Qiao W, Yang W, Liu X. 2026. Regulatory network of leaf senescence in Arabidopsis: the roles of transcription factors and microRNAs. Int J Mol Sci. 27:3584).</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13650777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}