Plant Physiology最新文献

{"title":"Conserved language of plant gene regulation.","authors":"Neeta Lohani","doi":"10.1093/plphys/kiaf263","DOIUrl":"10.1093/plphys/kiaf263","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12225659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of transgene-free homozygous early-flowering poplar via cytosine base editing.","authors":"Ling Wu, Lichi Zhong, Hongju Xiao, Fuliang Cao, Qiang Cheng","doi":"10.1093/plphys/kiaf290","DOIUrl":"https://doi.org/10.1093/plphys/kiaf290","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"198 3","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of TaWRKY27 in the arms race between wheat and stripe rust.","authors":"Ritu Singh","doi":"10.1093/plphys/kiaf259","DOIUrl":"10.1093/plphys/kiaf259","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12230710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blue-light only: How horsetails broke the rules of stomatal control.","authors":"Ritu Singh, Erin Cullen","doi":"10.1093/plphys/kiaf298","DOIUrl":"10.1093/plphys/kiaf298","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12275086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Divide and survive: PuZFP1 coordinates dual root developmental pathways for drought adaptation in Populus.","authors":"Héctor H Torres-Martínez","doi":"10.1093/plphys/kiaf261","DOIUrl":"10.1093/plphys/kiaf261","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12225661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ETHYLENE INSENSITIVE2-like protein mediates submergence and drought responses in Physcomitrium patens","authors":"Md Masudul Karim, Mousona Islam, Marcos Takeshi Miyabe, Yuko Ikeda, Mohammed Arif Sadik Polash, Kanata Hirota, Hidetoshi Sakayama, Yoichi Sakata, Daisuke Takezawa","doi":"10.1093/plphys/kiaf293","DOIUrl":"https://doi.org/10.1093/plphys/kiaf293","url":null,"abstract":"ETHYLENE INSENSITIVE 2 (EIN2) is an Nramp family transmembrane protein recognized as an essential component of ethylene signaling in angiosperms. However, its functions in other plant systems are not fully understood. Here, we demonstrate that ppein2ab mutants of the moss Physcomitrium patens, in which both EIN2-like genes have been disrupted, do not show a typical ethylene-mediated escape response following submergence. Interestingly, ppein2ab mutants showed reduced sensitivity to abscisic acid (ABA), a phytohormone that mediates drought stress responses. The ppein2ab plants were sensitive to hyperosmosis and freezing stress and exhibited reduced late embryogenesis abundant protein accumulation. Furthermore, ppein2ab mutants showed reduced activation of both SNF1-related protein kinase2 (SnRK2), the central activator of ABA and osmostress signaling, and the B3-RAF kinase ARK/PpCTR1L, a positive regulator of SnRK2. These results indicate that EIN2 is a dual function signaling component mediating both submergence and drought signaling in bryophytes. The diminished ABA responses in ppein2ab were restored by introduction of Arabidopsis EIN2 and the EIN2 orthologs of the Charophyceaen alga Chara braunii, suggesting functional conservation of EIN2 orthologs in Phragmoplastophyta.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"26 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144533073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The susceptibility factor RTP1 negatively regulates Arabidopsis immunity by interacting with the cytochrome P450 protein CYP71B3","authors":"Yushu Wei, Deqian Zong, Yaling Tang, Lehui Kong, Xianxian Gao, Xiaoxue Wang, Yingqi Zhang, Yang Yang, Xiaoyu Qiang, Weixing Shan","doi":"10.1093/plphys/kiaf284","DOIUrl":"https://doi.org/10.1093/plphys/kiaf284","url":null,"abstract":"Oomycetes, particularly Phytophthora species, cause destructive plant diseases that severely threaten sustainable crop production. Due to the loss of genotype-specific disease resistance, it is important to identify and understand immune factors that mediate plant susceptibility. Loss-of-function of the susceptibility factor Resistance To Phytophthora parasitica 1 (RTP1) leads to broad-spectrum disease resistance in Arabidopsis thaliana(Arabidopsis thaliana (L.) Heynh.). Through RNA-seq analysis, we determined that CYP71B3, encoding an uncharacterized P450 enzyme, is significantly up-regulated in rtp1 mutant plants infected with P. parasitica. Loss-of-function of CYP71B3 led to abolished pathogen-associated molecular pattern (PAMP)-triggered oxidative burst and rendered Arabidopsis more susceptible to diverse pathogens, including the oomycete P. parasitica and bacterial Pseudomonas syringae. Conversely, overexpression of CYP71B3 enhanced plant resistance and PAMP-triggered oxidative burst. CYP71B3 localized in the endoplasmic reticulum and was destabilized by interacting with RTP1 via the I-38 residue, which is essential for its immune function and P450 enzyme activity. The expression of CYP71B3 was regulated by transcription factor bZIP60, which is required for rtp1-mediated resistance to P. parasitica. Our studies indicate that RTP1 mediates plant susceptibility by destabilizing the downstream positive immune factor CYP71B3.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"51 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144533076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Insights into DNA Recognition by HD-Zip Transcription Factors","authors":"Wan Chen, Wei Yan, Kai Jiang, Hongda Huang","doi":"10.1093/plphys/kiaf292","DOIUrl":"https://doi.org/10.1093/plphys/kiaf292","url":null,"abstract":"Homeodomain–leucine zipper (HD-Zip) genes encode a large family of plant-specific transcription factors (TFs) that are integral to plant development, growth, regulation, and responses to environmental and hormonal signals. While the roles and mechanisms of HD-Zip TFs have been extensively studied, the structural basis for their DNA recognition remains unclear. In this study, we analyzed DAP-seq data and identified consensus DNA motifs, 5'-AAT[W]AT-3' and 5'-[N]AAA[N][N]-3', preferentially bound by HD-Zip TFs. Both motifs feature a 5'-AA(T/A)-3' core, which is shared across previously identified HD-Zip target sequences, suggesting a common recognition feature within the HD-Zip family. Focusing on the well-characterized HD-Zip IV TF PROTEIN PRODUCTION FACTOR 2 (PDF2) from Arabidopsis (Arabidopsis thaliana) and its interaction with the L1 box DNA sequence, our structural and biochemical analyses revealed that the PDF2 HD-ZA module forms a dimer to specifically recognize the 5'-AATG-3' core through an asymmetric binding mode. In this mode, only the primary recognition helix of one protomer and the N-arm of the other protomer in the PDF2 HD-ZA dimer are involved in specific DNA interactions. Our study offers insights into the molecular mechanisms of HD-Zip TFs and provides a structural template for engineering applications in agricultural research.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"79 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144516018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-regulated dual-targeting of NUCLEAR CONTROL OF PEP ACTIVITY establishes photomorphogenesis via interorganellar communication","authors":"Jae-Hyung Lee, Thu Minh Doan, Abigail Bruzual, Sandhya Senthilkumar, Chan Yul Yoo","doi":"10.1093/plphys/kiaf289","DOIUrl":"https://doi.org/10.1093/plphys/kiaf289","url":null,"abstract":"Interorganellar communication is essential for maintaining cellular and organellar functions and adapting to dynamic environmental changes in eukaryotic cells. In angiosperms, light initiates photomorphogenesis, a developmental program characterized by chloroplast biogenesis and inhibition of hypocotyl elongation, through photoreceptors such as the red/far-red-sensing phytochromes and their downstream signaling pathways. However, the mechanisms underlying nucleus-chloroplast crosstalk during photomorphogenesis remain elusive. Here, we show that light-regulated dual-targeting of NUCLEAR CONTROL OF PEP ACTIVITY (NCP) mediates bidirectional communication between the nucleus and chloroplasts via alternative promoter selection and retrograde translocation. Light promotes transcription from an upstream canonical transcription start site, producing a long NCP isoform (NCP-L) containing an N-terminal chloroplast transit peptide that directs chloroplast localization. In contrast, darkness or low red-light conditions favor transcription from a downstream alternative start site, producing a shorter cytoplasmic isoform (NCP-S) that is rapidly degraded via the 26S proteasome. This light-regulated alternative transcription initiation depends on PHYTOCHROME-INTERACTING FACTORS (PIFs), key repressors of photomorphogenesis. Upon chloroplast import, NCP-L is processed into its mature form (NCPm), which promotes assembly and nucleoid localization of the PEP complex to initiate chloroplast biogenesis. Notably, NCP’s nuclear function requires its prior localization to chloroplasts, supporting a model in which NCP mediates chloroplast-to-nucleus retrograde signaling. Consistent with this, NCP promotes stromule formation in Arabidopsis (Arabidopsis thaliana) hypocotyls, linking chloroplast dynamics to phytochrome-dependent nuclear pathways that restrict hypocotyl elongation. Our findings uncover an interorganellar communication mechanism in which light-dependent alternative promoter usage and retrotranslocation regulate photomorphogenesis, integrating nuclear and plastid signals to coordinate organ-specific developmental programs.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"33 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144521175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Transcription Factor LpWRKY65 Enhances Embryogenic Capacity through ROS Scavenging during Somatic Embryogenesis of Larch","authors":"Xiaoyi Chen, Luyao Zhang, Chengbi Liu, Rui Wang, Jianfeng Dai, Lisheng Kong, Jinfeng Zhang, Jian Zhao","doi":"10.1093/plphys/kiaf286","DOIUrl":"https://doi.org/10.1093/plphys/kiaf286","url":null,"abstract":"Somatic embryogenesis is a powerful system for studying embryo development and scaling up the production of elite genetic material. Somatic embryogenesis has been well established in Larix principis-rupprechtii, a Chinese larch species dominant in the world’s largest man-made forest. However, genotype-dependent embryogenic variations hinder large-scale forestry, and the molecular mechanisms remain unclear. Here, we constructed stage-specific developmental transcriptomes of the somatic embryogenesis process using two lines with contrasting embryogenic capacities. Clustering and co-expression analyses identified LpWRKY65 as a central hub gene highly expressed in early somatic embryogenesis stages and with significantly higher expression in the high-embryogenic-capacity cell line (HEL) compared to the low-embryogenic-capacity cell line (LEL). Overexpressing LpWRKY65 significantly increased somatic embryo yield and quality. DAP-seq and RNA-seq were combined to identify a set of target genes downstream of and responsive to LpWRKY65, particularly including genes involved in reactive oxygen species (ROS) scavenging. We identified LpHmgB10 as a critical downstream regulator of LpWRKY65. LpWRKY65 directly binds to the W-box in the promoter of LpHmgB10, markedly enhancing its transcriptional activity. ROS profiling further demonstrated that overexpression of LpWRKY65 or LpHmgB10 enhances ROS scavenging and promotes a stable redox environment, which is crucial for improving embryogenic capacity. These findings suggest that LpWRKY65 regulates the cellular redox environment to promote embryogenic differentiation and somatic embryo development, advancing somatic embryogenesis research in conifers.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"66 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144520839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}