Plant Physiology最新文献_第5页

A defense-inducible bidirectional promoter enables disease-resistance engineering in Arabidopsis without growth trade-offs. 防御诱导的双向启动子使拟南芥的抗病工程无需生长权衡。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-21 DOI: 10.1093/plphys/kiag237

Xiao-Xia Lin,Chong Li,Ben-Qiang Gong,Jun-Jie Liu,Lu-Yuan Zhu,Kehan Tao,Dandan Zhang,Jian-Feng Li

{"title":"A defense-inducible bidirectional promoter enables disease-resistance engineering in Arabidopsis without growth trade-offs.","authors":"Xiao-Xia Lin,Chong Li,Ben-Qiang Gong,Jun-Jie Liu,Lu-Yuan Zhu,Kehan Tao,Dandan Zhang,Jian-Feng Li","doi":"10.1093/plphys/kiag237","DOIUrl":"https://doi.org/10.1093/plphys/kiag237","url":null,"abstract":"The defense-growth trade-off remains a central challenge in crop breeding. For molecular breeding of crop disease resistance, immune-inducible promoters are valuable tools, as constitutive expression of defense-related genes often leads to growth defects. In this study, through transcriptome analysis and reporter-based screening, we identified BiPro1, a compact (313 bp) defense-inducible bidirectional promoter from Arabidopsis thaliana, which is responsive to elicitors such as the bacterial flagellin peptide flg22 and fungal chitin oligosaccharides. To demonstrate its utility, we used BiPro1 to precisely replace the intergenic region between two head-to-head nucleotide-binding leucine-rich repeat (NLR) genes, RRS1 and RPS4, via CRISPR/Cas9-mediated homologous recombination (HR). The HR-edited plants exhibited normal growth under unchallenged conditions but displayed markedly enhanced induction of both RRS1 and RPS4 under elicitor treatment, accompanied by significantly elevated immune responses. This endowed BiPro1 knock-in plants with broad-spectrum resistance to both bacterial and fungal pathogens, including Pseudomonas syringae, Ralstonia solanacearum, Botrytis cinerea, and Verticillium dahliae. These findings establish BiPro1 as a useful regulatory switch for disease resistance engineering in plants and provide an NLR-based strategy to generate crops with enhanced disease resistance while minimizing growth penalties.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"426 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147731373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SlMYB30 relieves high vapor pressure deficit stress by modulating salicylic acid biosynthesis and stomatal morphology in tomato. SlMYB30通过调节番茄水杨酸生物合成和气孔形态来缓解高蒸汽压亏缺胁迫。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-21 DOI: 10.1093/plphys/kiag233

Min Jia,Zhi-Peng Yu,Fei Liu,Guo-Bin Li,Gao-Xiang Zhang,Shu-Hui Zhang,Jian-Ming Li

{"title":"SlMYB30 relieves high vapor pressure deficit stress by modulating salicylic acid biosynthesis and stomatal morphology in tomato.","authors":"Min Jia,Zhi-Peng Yu,Fei Liu,Guo-Bin Li,Gao-Xiang Zhang,Shu-Hui Zhang,Jian-Ming Li","doi":"10.1093/plphys/kiag233","DOIUrl":"https://doi.org/10.1093/plphys/kiag233","url":null,"abstract":"High vapor pressure deficit (HVPD) stress presents challenges to global agriculture, impacting crop productivity, water resource management, ecosystem stability, and food security. Tomato (Solanum lycopersicum L.) is an economically important crop grown extensively around the world; however, the molecular mechanisms that enable its resistance to HVPD stress are not yet fully understood. In this study, V-MYB AVIAN MYELOBLASTOSIS VIRAL ONCOGENE HOMOLOG 30 (SlMYB30), an R2R3-type MYB transcription factor responsive to HVPD stress in tomato, was identified through transcriptomic and metabolomic analyses. We examined the role of SlMYB30 in conferring resistance to HVPD stress using transgenic plants. Genetic and physiological analyses demonstrated that SlMYB30 enhances tomato resistance to HVPD stress by boosting the plant's antioxidant capacity and optimizing stomatal morphology. Furthermore, SlMYB30 expression was induced by exogenous salicylic acid (SA), suggesting its involvement in SA signaling or a potential feedback regulatory loop. Under HVPD conditions, tomato plants also showed increased transcription of ISOCHORISMATE SYNTHASE (ICS), a key enzyme involved in SA biosynthesis. Interaction assays revealed that SlMYB30 binds to the MBS motifs within the SlICS promoter, leading to its activation and an increase in SA accumulation, thereby enhancing plant adaptation to HVPD stress. In addition, SlMYB30 positively regulated the expression of the PLASMA MEMBRANE INTRINSIC PROTEIN 1;7 (SlPIP1;7), affecting stomatal morphology and boosting antioxidant enzyme activity. This study elucidates the crucial role that the SlMYB30-mediated SA synthesis pathway plays in conjunction with the aquaporin SlPIP1;7 during HVPD stress, offering molecular targets for breeding crops with enhanced HVPD stress resistance.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"5 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147731375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A New Player at the Tip: KPZP Steers Pollen Tube Growth by Reining in Receptor Trafficking. 新参与者：KPZP通过控制受体贩运来引导花粉管生长。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-21 DOI: 10.1093/plphys/kiag226

Neeta Lohani,María Flores-Tornero

引用次数: 0

Multi-omics and genetic analysis uncover salicylic acid-mediated core signaling networks for seed longevity in rice 多组学和遗传分析揭示了水杨酸介导的水稻种子长寿核心信号网络

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-20 DOI: 10.1093/plphys/kiag184

Chengliang Wang, Jingjing Zhu, Chaofei Han, Qianqian Song, Zihan Li, Wei Jiang, Tangjuan Wu, Qian Cheng, Yuanyuan Wang, Wei Jin, Kai Sun, Hugh W Pritchard, Weixiang Lv, Sheng Zuo, Zhong-Hua Chen

{"title":"Multi-omics and genetic analysis uncover salicylic acid-mediated core signaling networks for seed longevity in rice","authors":"Chengliang Wang, Jingjing Zhu, Chaofei Han, Qianqian Song, Zihan Li, Wei Jiang, Tangjuan Wu, Qian Cheng, Yuanyuan Wang, Wei Jin, Kai Sun, Hugh W Pritchard, Weixiang Lv, Sheng Zuo, Zhong-Hua Chen","doi":"10.1093/plphys/kiag184","DOIUrl":"https://doi.org/10.1093/plphys/kiag184","url":null,"abstract":"Seed longevity is a critical agronomic trait underpinning crop productivity, yet its regulation remains less understood due to the complexity of interacting signaling pathways. Here, we identified an aging-resistant rice (Oryza sativa L.) cultivar ZH11 that shows a higher seed germination rate and vigor, higher salicylic acid (SA) content, and lower reactive oxygen species (ROS) level than other cultivars (NJ505 and XF9) under aging treatment. Integrated multi-omics analyses revealed six key pathways—oxidative phosphorylation, plant-pathogen interactions, photosynthesis, carbon fixation, ATP-binding cassette (ABC) transport, and hormone signaling—converging on nine core genes (OsUbOR, OsA10, WRKY42, OsPKD, OsPRCW, OsYGD1-1, OsABCB14, OsPYL3, OsNPR1). Deficiency of each of the nine key genes leads to mutants with a phenotype of markedly reduced seed viability. The differential expression of these key genes across ZH11, NJ505, and XF9, together with the dose-dependent rescue of the shortened seed longevity phenotype in NJ505 and XF9 by SA, confirms that SA acts as a key bridge mediating distinct signaling pathways. The interactions between the SA receptor NPR1 (Nonexpressor of Pathogenesis-Related 1) and bZIP03/bZIP28 (Basic Leucine Zipper 03/28) were identified as potential targets and components for regulating seed longevity. Furthermore, the interactions of NPR1-Protein Phosphatase 2C 30 (PP2C30) and PP2C30-bZIP28 further underscored the role of SA signaling in the aging process of rice seeds. Our findings provide mechanistic insights into seed viability regulation and offer genetic targets for breeding crops with extended seed storage potential and quality for sustainable agriculture.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"23 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147726048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grain-maturing temperature induces seed epi-memory via DNA methylation for subsequent development in rice ( Oryza sativa L.) 水稻籽粒成熟温度通过DNA甲基化诱导种子外记忆，促进后续发育

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-20 DOI: 10.1093/plphys/kiag219

Chetphilin Suriyasak, Yui Oyama, Ryusuke Kawaguchi, Ryo Matsumoto, Yuta Sawada, Wun-Jin Chen, Hue Thi Nong, Norimitsu Hamaoka, Yushi Ishibashi

{"title":"Grain-maturing temperature induces seed epi-memory via DNA methylation for subsequent development in rice ( Oryza sativa L.)","authors":"Chetphilin Suriyasak, Yui Oyama, Ryusuke Kawaguchi, Ryo Matsumoto, Yuta Sawada, Wun-Jin Chen, Hue Thi Nong, Norimitsu Hamaoka, Yushi Ishibashi","doi":"10.1093/plphys/kiag219","DOIUrl":"https://doi.org/10.1093/plphys/kiag219","url":null,"abstract":"High temperature during grain filling diminishes the yield and quality of rice (Oryza sativa L.), but whether and how it affects development and agronomic traits in the subsequent plants remain unclear. In this study, we dissected the DNA methylome of seeds that developed under heat stress (heat-treated during development seeds, HDS) or control seeds (CS), and the transcriptomes of the plants derived from these seeds. Our methylome analysis identified 457 differentially methylated regions in HDS, mostly at the promoter regions of protein-coding genes and transposons. Transcriptome analysis detected 2,824 differentially expressed genes between plants grown from HDS or CS. HDS-derived subsequent plants were shorter, produced more tillers, had greater stomatal density, flowered earlier, and exhibited earlier diurnal floret opening than CS-derived plants. We linked differentially expressed genes to these phenotypic changes: OsSLB1 and OsYODA1 were downregulated, promoting abundant tillering and high stomatal density, respectively; OsHd1 and OsAOC1 were upregulated, leading to early flowering and early diurnal floret opening, respectively. In HDS, the OsSLB1 and OsYODA1 promoters were hypermethylated, but the OsHd1 and OsAOC1 promoters were hypomethylated, relative to CS. Notably, these seed methylation differences remained in their derived subsequent plants. Finally, yield was 9.5% higher for HDS-derived plants than for CS-derived plants when cultivated in the field. Our findings suggest the importance of seed epigenetic memory, mediated by DNA methylation, for the development of preferred agronomic traits. Alterations in seed DNA methylation due to grain-maturing temperature induce favorable adaptive traits in rice","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"69 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147726045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atypical endo-β-1,4-mannanases are necessary for normal glucomannan synthesis in Arabidopsis. 非典型内切-β-1,4-甘露聚糖酶是拟南芥合成正常葡甘露聚糖所必需的酶。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-04-20 DOI: 10.1093/plphys/kiag174

Aina Kikuchi, Eriko Sato, Yoshihisa Yoshimi, Hironori Takasaki, Naho Nishigaki, Kimie Atsuzawa, Yasuko Kaneko, Masatoshi Yamaguchi, Daisuke Takahashi, Paul Dupree, Toshihisa Kotake

{"title":"Atypical endo-β-1,4-mannanases are necessary for normal glucomannan synthesis in Arabidopsis.","authors":"Aina Kikuchi, Eriko Sato, Yoshihisa Yoshimi, Hironori Takasaki, Naho Nishigaki, Kimie Atsuzawa, Yasuko Kaneko, Masatoshi Yamaguchi, Daisuke Takahashi, Paul Dupree, Toshihisa Kotake","doi":"10.1093/plphys/kiag174","DOIUrl":"https://doi.org/10.1093/plphys/kiag174","url":null,"abstract":"<p><p>The molecular mechanisms underlying the synthesis of large cell wall polysaccharides in plant cells are not fully understood. Here we report that two atypical endo-β-1,4-mannanases (MANs), which are not secreted and do not degrade glucomannan in the cell wall, play a role in glucomannan synthesis. Among the six MANs in Arabidopsis (Arabidopsis thaliana), AtMAN2 and AtMAN5 contain a transmembrane domain at their N-terminal region instead of a signal peptide. Subcellular localization using MAN protein fused to a fluorescent protein demonstrated that AtMAN2 localizes to the endomembrane system, including the Golgi apparatus, in xylem and interfascicular fiber cells. An Arabidopsis man2 man5 double mutant lost 65% of glucomannan in the cell walls of the inflorescence stem. Immunostaining and immunoelectron microscopic observation also revealed that the man2 man5 double mutant loses glucomannan in the cell walls to about the same extent as the csla2 csla9 double mutant, which lacks major glucomannan synthases. Gene complementation experiments showed that the enzymatic activities of AtMAN2 and AtMAN5 are important for the synthesis of cell wall glucomannan. Arabidopsis possesses another atypical MAN, AtMAN6, with an HDEL retention signal at its C-terminus. However, mutation of AtMAN6 did not affect glucomannan content in the cell walls, suggesting distinct functions for these MANs. This study has identified AtMAN2 and AtMAN5 as factors necessary for normal glucomannan synthesis in Arabidopsis, along with GDP-mannose-generating enzymes and CslAs, and suggests that glucomannan hydrolysis by these MANs contributes to maintaining glucomannan synthesis.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147729650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gas exchange measurements of carboxysome mutants reveal insights into cyanobacterial carbon concentrating mechanisms 羧基体突变体的气体交换测量揭示了蓝藻碳浓缩机制的见解

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-18 DOI: 10.1093/plphys/kiag221

Rees Rillema, Anne Steensma, Berkley J Walker, Daniel C Ducat

{"title":"Gas exchange measurements of carboxysome mutants reveal insights into cyanobacterial carbon concentrating mechanisms","authors":"Rees Rillema, Anne Steensma, Berkley J Walker, Daniel C Ducat","doi":"10.1093/plphys/kiag221","DOIUrl":"https://doi.org/10.1093/plphys/kiag221","url":null,"abstract":"Gas exchange measurements are widely used in plant research to quantitatively resolve photosynthetic performance and CO2 assimilation rates, yet gas exchange techniques are less commonly applied to algae and cyanobacteria. Cyanobacteria have a well-characterized carbon concentrating mechanism (CCM) that includes a proteinaceous microcompartment called the carboxysome. Carboxysome structural components have been extensively studied and consist of a nucleated core of rubisco encapsulated by a diverse family of structurally related shell proteins. In addition to the dominant shell proteins that compose the majority of carboxysome facets, accessory shell components are widely distributed throughout cyanobacterial phyla. Herein, we utilize gas exchange techniques to elucidate the photosynthetic impacts of carboxysome mutants in Synechoccocus elongatus PCC 7942, emphasizing analysis of three accessory shell proteins: CcmK3, CcmK4, and CcmP. We detail technical considerations important for increasing the throughput and accuracy of gas exchange measurements using a recently developed commercial aquatic chamber. We find distinct photosynthetic phenotypes associated with the roles of different accessory shell proteins, with context-specific photosynthetic impairment in ΔccmP mutants that contrast with the broader pleiotropic defects of ΔccmK3 ΔccmK4 strains under multiple environments. Our results have implications for understanding the role of accessory carboxysome shell components in facilitating carbon fixation and other carboxysomal functions.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"67 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147726046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular insights into avocado grafting: the role of chitinase-like genes. 分子洞察鳄梨嫁接：几丁质酶样基因的作用。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-17 DOI: 10.1093/plphys/kiag187

Vikas Dwivedi,Lalita Pal,Gal Hadas-Brandwein,Sela Yechezkel,Alexander Klaus,Adi Faigenboim-Doron,Eduard Belausov,Balaji U Solanke,Roy Weinstain,Einat Sadot

{"title":"Molecular insights into avocado grafting: the role of chitinase-like genes.","authors":"Vikas Dwivedi,Lalita Pal,Gal Hadas-Brandwein,Sela Yechezkel,Alexander Klaus,Adi Faigenboim-Doron,Eduard Belausov,Balaji U Solanke,Roy Weinstain,Einat Sadot","doi":"10.1093/plphys/kiag187","DOIUrl":"https://doi.org/10.1093/plphys/kiag187","url":null,"abstract":"Grafting is an agricultural technique used widely to increase yield, enhance stress tolerance, and improve disease resistance. The valuable avocado (Persea americana Mill.) rootstock VC801 provides these benefits but exhibits poor rooting capacity, necessitating a labor-intensive double-grafting procedure that involves a nursing seedling and etiolation. In this study, we found that grafting of the 'Hass' cultivar onto VC801 was successful only when the rootstocks were etiolated; by contrast, grafts on green rootstocks largely failed, indicating physiological incompatibility. Transcriptome analysis revealed that graft success was associated with differential expression of cell wall-related genes, among which chitinase-like transcripts were particularly prominent. Repeated RT-qPCR validation highlighted the Ctg0022 chitinase-like transcript. The Arabidopsis (Arabidopsis thaliana) mutant ctl1-1, which is defective in a homologous chitinase-like gene, displays graft incompatibility. Remarkably, heterologous expression of Ctg0022, particularly in the rootstock, restored graft compatibility in these mutants. Complementary analyses, including inhibitor treatments, histology, microscopy, and gene expression profiling, indicated that Ctg0022 modulates cell wall remodeling by influencing cellulose, lignin, and pectin composition. Fluorescence lifetime imaging further showed aberrant cell wall assemblies and impaired cell division and cell adhesion at the graft interface of ctl1-1 mutants; these were restored in Ctg0022-expressing plants. Together, our findings demonstrate that the fine-tuned expression of avocado chitinase-like gene Ctg0022 can promote successful graft union formation, highlighting a conserved role in cell wall remodeling during grafting.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"6 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147702037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the ancestral role of Class I HD-Zip transcription factors in land plants. 阐明I类HD-Zip转录因子在陆地植物中的祖先作用。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-17 DOI: 10.1093/plphys/kiag217

Facundo Romani,Santiago Farías,Ariel Cerda Rojas,Stevie N Florent,Philip Carella,Sebastian Schornack,John L Bowman,Fernan Federici,Javier E Moreno

{"title":"Elucidating the ancestral role of Class I HD-Zip transcription factors in land plants.","authors":"Facundo Romani,Santiago Farías,Ariel Cerda Rojas,Stevie N Florent,Philip Carella,Sebastian Schornack,John L Bowman,Fernan Federici,Javier E Moreno","doi":"10.1093/plphys/kiag217","DOIUrl":"https://doi.org/10.1093/plphys/kiag217","url":null,"abstract":"Class I homeodomain-leucine zipper (C1HDZ) transcription factors regulate diverse developmental and stress-related processes in land plants. While extensively studied in angiosperms, their functions in other lineages are poorly understood. In the liverwort Marchantia polymorpha, the sole C1HDZ orthologue (MpC1HDZ) controls herbivore defense by regulating oil body cell formation. This suggests that the function of C1HDZ genes may have diverged during the evolution of land plants. Here, we further investigated the function of MpC1HDZ in M. polymorpha and its integration into Arabidopsis (Arabidopsis thaliana) regulatory networks. Mpc1hdz mutants were more susceptible to herbivory by Spodoptera frugiperda caterpillars but displayed greater resistance to the oomycete Phytophthora palmivora, indicating that MpC1HDZ-mediated defenses extend to other herbivores but not oomycete pathogens. Loss-of-function Mpc1hdz mutants exhibited developmental alterations, including shortened gemma cups, diminished air pore density, and absence of surface papillae, in addition to the loss of oil bodies, highlighting a broader role for MpC1HDZ in epidermal cell differentiation. Heterologous expression of MpC1HDZ in the Arabidopsis athb13-2 mutant resulted in a smaller rosette diameter, greater leaf number and serration, and shortened inflorescence height; it also restored drought tolerance and improved the forage quality of the mutant tissues. These findings demonstrate that MpC1HDZ can be functionally integrated into endogenous HD-ZIP I regulatory networks, modulating C1HDZ-associated responses. Together, these results support an ancestral developmental role for C1HDZ genes, with abiotic stress functions emerging later in angiosperm evolution, enabled by conserved protein features that facilitate regulatory network integration.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"14 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147702038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brassinosteroid-mediated shoot branching in tomato requires SRT1-dependent histone deacetylation. 油菜素内酯介导的番茄茎分枝需要srt1依赖性组蛋白去乙酰化。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-17 DOI: 10.1093/plphys/kiag223

Xuewei Song,Lin Lu,Fang Feng,Yue Liu,Chuyong Chen,Ting Wang,Ping Yang,Yanhong Zhou,Jingquan Yu,Xiaojian Xia

{"title":"Brassinosteroid-mediated shoot branching in tomato requires SRT1-dependent histone deacetylation.","authors":"Xuewei Song,Lin Lu,Fang Feng,Yue Liu,Chuyong Chen,Ting Wang,Ping Yang,Yanhong Zhou,Jingquan Yu,Xiaojian Xia","doi":"10.1093/plphys/kiag223","DOIUrl":"https://doi.org/10.1093/plphys/kiag223","url":null,"abstract":"Shoot branching is a crucial agronomic trait influencing crop yield. Multiple transcription factors orchestrate a sophisticated regulatory network to control shoot branching in response to internal and external cues. However, the role of epigenetic modification in shoot branching is less clear. Here, we found that SIRTUIN1 (SlSRT1), a histone deacetylase, promotes shoot branching by regulating transcription in lateral buds in response to brassinosteroid (BR) signaling in tomato (Solanum lycopersicum). SlSRT1 interacted with BRASSINAZOLE-RESISTANT 1 (SlBZR1), a critical component of BR signaling, to repress the transcription of RELATED TO ABI3/VP1 1 (SlRAV1) and BRANCHED1 (SlBRC1) through decreasing H3K9 acetylation. SlBRC1 and SlRAV1 both inhibited shoot branching in tomato. Intriguingly, the genetic analysis, spatial-temporal expression of SlBRC1 and SlRAV1, and the transcriptomes in the buds of slbrc1 and slrav1 mutants indicated that SlBRC1 and SlRAV1 function through partially independent mechanisms. Our results demonstrate that SlSRT1-dependent histone deacetylation is essential for BR regulation of shoot branching.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"28 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147702039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0