Plant Physiology最新文献_第5页

AlGrow: A graphical interface for easy, fast, and accurate area and growth analysis of heterogeneously colored targets. AlGrow：图形界面，可对异色目标进行简便、快速、准确的面积和生长分析。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-23 DOI: 10.1093/plphys/kiae577

Marcus McHale, Ronan Sulpice

引用次数: 0

The bHLH transcription factor gene EGL3 accounts for the natural diversity in Arabidopsis fruit trichome pattern and morphology

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-12-22 DOI: 10.1093/plphys/kiae673

Belén Méndez-Vigo, Noelia Arteaga, Alba Murillo-Sánchez, Sonia Alba, Carlos Alonso-Blanco

{"title":"The bHLH transcription factor gene EGL3 accounts for the natural diversity in Arabidopsis fruit trichome pattern and morphology","authors":"Belén Méndez-Vigo, Noelia Arteaga, Alba Murillo-Sánchez, Sonia Alba, Carlos Alonso-Blanco","doi":"10.1093/plphys/kiae673","DOIUrl":"https://doi.org/10.1093/plphys/kiae673","url":null,"abstract":"The number and distribution of trichomes, i.e., the trichome pattern, in different plant organs shows a conspicuous inter- and intraspecific diversity across Angiosperms that is presumably involved in adaptation to numerous environmental factors. The genetic and molecular mechanisms accounting for the evolution of trichome patterns have just begun to be elucidated. In this study, we aimed to identify and characterize MALAMBRUNO 1 (MAU1), a locus affecting trichome number in the fruits of Arabidopsis (Arabidopsis thaliana) natural populations. To this end, we developed introgression lines (ILs) from the hairy fruit accession Don-0 in the genetic background of the Ler strain with glabrous fruits. Genetic analyses of ILs showed that MAU1 affects fruit trichome patterns through synergistic epistasis with the MYB genes TRICHOMELESS1 (TCL1), GLABRA1 (GL1), and TRIPTYCHON (TRY). In addition, fine mapping and characterization of transgenic lines demonstrated that MAU1 is the bHLH transcription factor gene EGL3, for which Don-0 carries a gain-of-function semidominant allele. Gene expression analyses did not detect differences between EGL3 alleles, thus supporting that a structural missense mutation is the causal nucleotide polymorphism of Don-0. Further phylogenetic analyses of EGL3 showed that most Arabidopsis populations with hairy fruits belong to three haplogroups, suggesting that additional EGL3 natural alleles account for fruit trichome development. Finally, the characterization of EGL3 pleiotropy indicates that Don-0 hyperfunction also increases stem trichome branching. We conclude that EGL3 interactions in the core gene regulatory network of trichome development explain the Arabidopsis natural diversity for fruit trichome pattern and morphology.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"61 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142874423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of abscisic acid receptor mRNA stability: involvement of microRNA5628 in PYL6 transcript decay

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-12-21 DOI: 10.1093/plphys/kiae663

João G P Vieira, Gustavo T Duarte, Carlos H Barrera-Rojas, Cleverson C Matiolli, Américo J C Viana, Raphael de A Campos, Lucas E D Canesin, Renato Vicentini, Fabio T S Nogueira, Michel Vincentz

{"title":"Regulation of abscisic acid receptor mRNA stability: involvement of microRNA5628 in PYL6 transcript decay","authors":"João G P Vieira, Gustavo T Duarte, Carlos H Barrera-Rojas, Cleverson C Matiolli, Américo J C Viana, Raphael de A Campos, Lucas E D Canesin, Renato Vicentini, Fabio T S Nogueira, Michel Vincentz","doi":"10.1093/plphys/kiae663","DOIUrl":"https://doi.org/10.1093/plphys/kiae663","url":null,"abstract":"Phytohormone signaling is fine-tuned by regulatory feedback loops. The phytohormone abscisic acid (ABA) plays key roles in plant development and abiotic stress tolerance. PYRABACTIN RESISTENCE 1/PYR1-LIKE/REGULATORY COMPONENT OF ABA RECEPTOR (PYR/PYL/RCAR) receptors sense ABA, and in turn, ABA represses their expression. Conversely, ABA induces expression of type 2C PROTEIN PHOSPHATASES (PP2C) genes, which negatively regulate the ABA signaling pathway. This regulatory feedback scheme is likely important for modulating ABA signaling. Here, we provide insight into the mechanisms underlying the ABA-induced repression of PYR/PYL/RCAR expression in Arabidopsis (Arabidopsis thaliana). ABA time course analyses revealed strong and sustained repression of PYR/PYL/RCARs, suggesting that receptor gene regulation is an important step in resetting the ABA signaling pathway. Cordycepin-induced transcription inhibition showed that PYL1/4/5/6 mRNA destabilization is involved in the ABA-induced repression of these genes. Furthermore, genetic evidence indicated that decapping may play a role in PYL4/5/6 mRNA decay. We also provide evidence that the Arabidopsis-specific microRNA5628 (miR5628), which is transiently induced by the ABA core signaling pathway, guides PYL6 transcript cleavage in response to ABA. After cleavage, the resulting 5’- and 3’-cleaved fragments of PYL6 mRNA may be degraded by the XRN4 exoribonuclease. miR5628 is an evolutionary novelty that may enhance PYL6 mRNA degradation, along with decapping and XRN4 activity. Thus, regulating the stability of PYR/PYL/RCAR transcripts maintains ABA signaling homeostasis.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calcium signaling in hypoxic response

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-12-21 DOI: 10.1093/plphys/kiae654

Arkadipta Bakshi, Simon Gilroy

{"title":"Calcium signaling in hypoxic response","authors":"Arkadipta Bakshi, Simon Gilroy","doi":"10.1093/plphys/kiae654","DOIUrl":"https://doi.org/10.1093/plphys/kiae654","url":null,"abstract":"Plants can experience a lack of oxygen due to environmental conditions such as flooding events or intense microbial blooms in the soil, and from their own metabolic activities. The associated limit on aerobic respiration can be fatal. Therefore, plants have evolved sensing systems that monitor oxygen levels and trigger a suite of metabolic, physiological and developmental responses to endure, or potentially escape, these oxygen limiting conditions. Low oxygen stress has long been known to trigger changes in cytosolic Ca2+ levels in plants and recent work has seen some major steps forward in characterizing these events as part of a Ca2+-based signaling system through: (1) defining how hypoxia may trigger and then shape the dynamics of these Ca2+ signals and, (2) identifying a host of the downstream elements that allow Ca2+ to regulate a wide-ranging network of hypoxia responses. Calcium transporters such as the CAX family of Ca2+/H+ antiporters at the tonoplast have emerged as important components of the system that forms hypoxia-related Ca2+ signals. Downstream lies a web of Ca2+-responsive proteins such as the calmodulin like proteins (CMLs), Ca2+-dependent kinases (CPKs), the calcineurin-B like proteins (CBLs) along with their interacting kinases (CIPKs). A host of other regulators such as reactive oxygen species and lipid-mediated signals then act in parallel to the Ca2+-dependent events to closely control and coordinate the myriad responses that characterize the plant’s low oxygen response.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"52 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis. MYB73 的相分离调节拟南芥种子油的生物合成。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-20 DOI: 10.1093/plphys/kiae674

Yuzhou Yang, Que Kong, Zhiming Ma, Peng Ken Lim, Sanjay K Singh, Sitakanta Pattanaik, Marek Mutwil, Yansong Miao, Ling Yuan, Wei Ma

{"title":"Phase separation of MYB73 regulates seed oil biosynthesis in Arabidopsis.","authors":"Yuzhou Yang, Que Kong, Zhiming Ma, Peng Ken Lim, Sanjay K Singh, Sitakanta Pattanaik, Marek Mutwil, Yansong Miao, Ling Yuan, Wei Ma","doi":"10.1093/plphys/kiae674","DOIUrl":"https://doi.org/10.1093/plphys/kiae674","url":null,"abstract":"MYB family transcription factors (TFs) play crucial roles in plant development, metabolism, and responses to various stresses. However, whether MYB TFs are involved in regulating fatty acid biosynthesis in seeds remains largely elusive. Here, we demonstrated that transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing MYB73 exhibit altered FATTY ACID ELONGATION1 (FAE1) expression, seed oil content, and seed fatty acid composition. Electrophoretic mobility shift assays (EMSAs) showed that FAE1 is a direct target of MYB73, and functional assays revealed that MYB73 represses FAE1 promoter activity. Transcriptomic analysis of the MYB73-overexpressing plants detected significant changes in the expression of genes involved in fatty acid biosynthesis and triacylglycerol assembly. Furthermore, MYB73 expression was responsive to abscisic acid (ABA), and ABA responsive element binding factor 2 (ABF2) directly bound to the ABA-responsive element (ABRE) in the MYB73 promoter to activate its expression. Additionally, we determined that MYB73 exhibits the hallmarks of an intrinsically disordered protein and forms phase-separated condensates with liquid-like characteristics, which are important in regulating target gene expression. Together, our findings suggest that MYB73 condensate formation likely fine-tunes seed oil biosynthesis.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CALMODULIN-BINDING RECEPTOR-LIKE CYTOPLASMIC KINASE 3 regulates salt tolerance through CATALASE 2 in Arabidopsis. 拟南芥中的 CALMODULIN-BINDING RECEPTOR-LIKE CYTOPLASMIC KINASE 3 通过 CATALASE 2 调节耐盐性。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-20 DOI: 10.1093/plphys/kiae669

Yufen Zhuang, Yiyi Zhang, Haifan Shi, Yanan Pang, Xixian Feng, Wenjuan Fan, Dan Chang, Honghui Lin, Huapeng Zhou

{"title":"CALMODULIN-BINDING RECEPTOR-LIKE CYTOPLASMIC KINASE 3 regulates salt tolerance through CATALASE 2 in Arabidopsis.","authors":"Yufen Zhuang, Yiyi Zhang, Haifan Shi, Yanan Pang, Xixian Feng, Wenjuan Fan, Dan Chang, Honghui Lin, Huapeng Zhou","doi":"10.1093/plphys/kiae669","DOIUrl":"https://doi.org/10.1093/plphys/kiae669","url":null,"abstract":"Soil salinization threatens global crop production. Here, we report that a receptor-like cytoplasmic kinase (RLCK), CALMODULIN-BINDING RECEPTOR-LIKE CYTOPLASMIC KINASE 3 (CRCK3), plays an essential role in plant salt tolerance via CATALASE 2 (CAT2), a hydrogen peroxide (H2O2)-scavenging enzyme in Arabidopsis (Arabidopsis thaliana). CRCK3 was induced by salt stress, and its knockout mutant displayed a salt-sensitive phenotype compared to wild-type (WT) plants. CRCK3 was activated by salt stress in a calcium-dependent manner, and its kinase activity was required for plant salt tolerance. CRCK3 physically interacted with CAT2, and CRCK3-mediated salt tolerance depended on CAT2. Salt treatment significantly induced CAT2 phosphorylation via the action of CRCK3, and this phosphorylation was required for CAT2-mediated H2O2 scavenging to reduce ROS content and oxidative damage in plants under saline conditions. CRCK3 phosphorylated CAT2 at the Thr209 residue, resulting in elevated catalase activity to reduce reactive oxygen species (ROS) accumulation under saline conditions. Therefore, the CRCK3-CAT2 module mediates plant salt tolerance by maintaining redox homeostasis. This study expands our knowledge of how plants respond to salt stress.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The brassinosteroid signaling-related ILI-OsAIF-OsbHLH92 transcription factor module antagonistically controls leaf angle and grain size in rice.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-20 DOI: 10.1093/plphys/kiae668

Mingmin Lu, Mingqian Liu, Qin Luo, Yubing He, Yanan Tian, Huadong Zhan

{"title":"The brassinosteroid signaling-related ILI-OsAIF-OsbHLH92 transcription factor module antagonistically controls leaf angle and grain size in rice.","authors":"Mingmin Lu, Mingqian Liu, Qin Luo, Yubing He, Yanan Tian, Huadong Zhan","doi":"10.1093/plphys/kiae668","DOIUrl":"https://doi.org/10.1093/plphys/kiae668","url":null,"abstract":"Atypical basic helix-loop-helix (bHLH) transcription factors, which lack the basic region for DNA binding, are important elements of brassinosteroid (BR) signaling. Recently, our systematic characterization of the rice (Oryza sativa) INCREASED LEAF INCLINATION (ILI) subfamily of atypical bHLHs revealed their indispensable roles in BR-mediated growth and development. Here, we reported the isolation of two additional rice ILI-interacting atypical bHLHs, ATBS1-INTERACTING FACTOR 1 (OsAIF1)/OsbHLH176 and OsAIF2/OsbHLH178. Genetic and cytological analyses of the OsAIFs knockout mutants and overexpression lines revealed that OsAIF1 and OsAIF2 negatively regulate rice leaf inclination and grain size in a synergistic and redundant manner. Compared to the wild type, osaif knockout mutants exhibited hypersensitivity to BR, while OsAIF1 and OsAIF2 overexpression lines showed greatly reduced sensitivity or complete insensitivity to BR, indicating that these two OsAIFs act as major negative regulators of rice BR signaling. As ILI-interacting negative atypical HLHs, OsAIF1 and OsAIF2 genetically counteracted the positive ILI subfamily of atypical HLHs. Moreover, OsAIF1 and OsAIF2 physically interacted with and antagonized OsbHLH92, a positive regulator of BR signaling, thereby modulating rice development and gene transcription. These findings suggested that the atypical HLHs (ILIs and OsAIF1/OsAIF2) and the bHLH (OsbHLH92) transcription factors form a triantagonistic cascade in rice, counteracting each other to fine-tune leaf angle and grain size through BR signaling. Our results provide insights into the mechanisms balancing BR signaling and growth in rice.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COBRA-LIKE 9 modulates cotton cell wall development via regulating cellulose deposition. COBRA-LIKE 9 通过调节纤维素沉积调节棉花细胞壁的发育。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-20 DOI: 10.1093/plphys/kiae675

Qingfei He, Yujia Yu, Zhiguang Qin, Yujia Duan, Hanqiao Liu, Weixi Li, Xiaohui Song, Guozhong Zhu, Xiaoguang Shang, Wangzhen Guo

{"title":"COBRA-LIKE 9 modulates cotton cell wall development via regulating cellulose deposition.","authors":"Qingfei He, Yujia Yu, Zhiguang Qin, Yujia Duan, Hanqiao Liu, Weixi Li, Xiaohui Song, Guozhong Zhu, Xiaoguang Shang, Wangzhen Guo","doi":"10.1093/plphys/kiae675","DOIUrl":"https://doi.org/10.1093/plphys/kiae675","url":null,"abstract":"Plant cell walls are complex and dynamic cellular structures critical for plant growth, development, physiology, and adaptation. Cellulose is one of the most important components of the cell wall. However, how cellulose microfibrils deposit and assemble into crystalline cellulose remains elusive. The COBRA-LIKE (COBL) plant-specific protein family plays vital roles in modulating the deposition and orientation of cellulose microfibril in plant cell walls. Here, we investigate the role of GhCOBL9 in cotton (Gossypium hirsutum) fiber development, an ideal model for studying cell elongation and cell wall thickening. The expression period of GhCOBL9 is consistent with the thickening stage of the secondary wall of cotton fibers. Overexpression of GhCOBL9 results in increased cellulose content in the cell wall and produces shorter, thicker, and stronger fibers, while RNA interference (RNAi)-mediated down-regulation of GhCOBL9 leads to the opposite phenotypes, indicating its crucial role in cell wall development. Subcellular localization and binding activity assays reveal that GhCOBL9 targets the cell wall and binds to crystalline cellulose with high affinity. Transcriptomic analysis of GhCOBL9 transgenic lines uncovers expression alterations in genes related to cellulose and monosaccharide biosynthesis. Furthermore, we identify a fasciclin-like arabinogalactan protein 9 (GhFLA9) as an interacting partner of GhCOBL9 to modulate cell wall development. Additionally, the R2R3-MYB transcription factor GhMYB46-5 activates GhCOBL9 expression by binding to the MYB46-responsive cis-regulatory element（M46RE）in the GhCOBL9 promoter. These findings broaden our knowledge of COBL function in modulating plant cell wall development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor ORA59 represses hypoxia responses during Botrytis cinerea infection and reoxygenation. 转录因子 ORA59 可抑制灰霉病菌感染和复氧过程中的缺氧反应。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-20 DOI: 10.1093/plphys/kiae677

Luca Brunello, Alicja B Kunkowska, Emma Olmi, Paolo M Triozzi, Simone Castellana, Pierdomenico Perata, Elena Loreti

{"title":"The transcription factor ORA59 represses hypoxia responses during Botrytis cinerea infection and reoxygenation.","authors":"Luca Brunello, Alicja B Kunkowska, Emma Olmi, Paolo M Triozzi, Simone Castellana, Pierdomenico Perata, Elena Loreti","doi":"10.1093/plphys/kiae677","DOIUrl":"https://doi.org/10.1093/plphys/kiae677","url":null,"abstract":"Transcription factors belonging to the large Ethylene Responsive Factor (ERF) family are involved in plant responses to biotic and abiotic stresses. Among the ERFs, OCTADECANOID-RESPONSIVE ARABIDOPSIS 59 (ORA59) integrates ethylene and jasmonic acid signaling to regulate resistance to necrotrophic pathogens. The ERF group ERFVII encodes oxygen-labile proteins that are required for oxygen sensing and are stabilized by hypoxia established at the site of Botrytis (Botrytis cinerea) infection. Here, we show that ORA59 represses ERFVII protein activity to induce the expression of hypoxia-responsive genes in Arabidopsis (Arabidopsis thaliana). Moreover, inhibition of ethanol fermentation enhances plant tolerance to Botrytis, indicating a trade-off between the hypoxia and defense responses. In addition, ERFVII members and ORA59 are both involved in the downregulation of hypoxia-responsive genes during reoxygenation. Taken together, our results reveal that the ERFVII transcription factor-ORA59 module ensures that the multiple roles of ERFVII proteins are correctly balanced to favor plant tolerance to biotic or abiotic stresses.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The calcium-dependent protein kinase CmaCPK4 regulates sex determination in pumpkin (Cucurbita maxima D.)

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-12-19 DOI: 10.1093/plphys/kiae666

Chaojie Wang, Yunli Wang, Guichao Wang, Ke Zhang, Zhe Liu, Xiaopeng Li, Wenlong Xu, Zheng Li, Shuping Qu

{"title":"The calcium-dependent protein kinase CmaCPK4 regulates sex determination in pumpkin (Cucurbita maxima D.)","authors":"Chaojie Wang, Yunli Wang, Guichao Wang, Ke Zhang, Zhe Liu, Xiaopeng Li, Wenlong Xu, Zheng Li, Shuping Qu","doi":"10.1093/plphys/kiae666","DOIUrl":"https://doi.org/10.1093/plphys/kiae666","url":null,"abstract":"Pumpkin (Cucurbita maxima D.) is typically monoecious with individual male and female flowers, and its yield is associated with the degree of femaleness, i.e. the ratio of female to male flowers produced by the plant. Subgynoecy represents a sex form with a high degree of femaleness, but the regulatory mechanisms in pumpkin remain poorly understood. In this study, using the F2 population crossed from the subgynoecious line 2013-12 and the monoecious line 9-6, we initially identified a recessive locus to control the subgynoecious trait, and named it sg1. After bulked segregant analysis with whole-genome resequencing (BSA-seq) and molecular marker linkage analysis, the sg1 locus was mapped to pumpkin chromosome 2. Genetic sequence analysis found a pumpkin calcium-dependent protein kinase (CPK) gene, CmaCPK4, in the mapping interval as the candidate gene. A retrotransposon insertion identified within the promoter elevated CmaCPK4 expression in 2013-12. Morphological characterization of near-isogenic lines (NILs) containing the sg1 allele showed increases in the ratio of female flowers and high ethylene contents in terminal buds compared to the receptor parent. Heterologous overexpression of CmaCPK4 significantly increased the ratio of female flowers in cucumber (Cucumis sativus). Furthermore, CmaCPK4 directly interacts with and phosphorylates 1-aminocyclopropane-1-carboxylate synthase 5 (CmaACS5) and 1-aminocyclopropane-1-carboxylate synthase 7 (CmaACS7), resulting in increased ethylene content in 2013-12, which affected pumpkin sex determination. These findings provide insights into the role of the CmaCPK4-CmaACS5/CmaACS7 module in ethylene-induced sex determination in pumpkin.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"31 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142858395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0