Plant Physiology最新文献_第6页

A conserved protein family in mirid bug Riptortus pedestris plays dual roles in regulating plant immunity. 镜蝽（Riptortus pedestris）中的一个保守蛋白家族在调节植物免疫方面发挥着双重作用。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-02 DOI: 10.1093/plphys/kiae468

Jiangxuan Zhou, Zhiyuan Yin, Danyu Shen, Qingsong Zhang, Yujie OYang, Xiaoxi Li, Yurong Ma, Lanping Ding, Yong Pei, Gan Ai, Yumei Dong, Donglei Yang, Yuanchao Wang, Daolong Dou, Ai Xia

{"title":"A conserved protein family in mirid bug Riptortus pedestris plays dual roles in regulating plant immunity.","authors":"Jiangxuan Zhou, Zhiyuan Yin, Danyu Shen, Qingsong Zhang, Yujie OYang, Xiaoxi Li, Yurong Ma, Lanping Ding, Yong Pei, Gan Ai, Yumei Dong, Donglei Yang, Yuanchao Wang, Daolong Dou, Ai Xia","doi":"10.1093/plphys/kiae468","DOIUrl":"10.1093/plphys/kiae468","url":null,"abstract":"The mirid bug (Riptortus pedestris), a major soybean pest, migrates into soybean fields during the pod filling stage and causes staygreen syndrome, which leads to substantial yield losses. The mechanism by which R. pedestris elicits soybean (Glycine max) defenses and counter-defenses remains largely unexplored. In this study, we characterized a protein family from R. pedestris, designated R.pedestris HAMP 1 (RPH1), and its putative paralogs (RPH1L1, 2, 3, 4, and 5), whose members exhibit dual roles in triggering and inhibiting plant immunity. RPH1 and RPH1L1 function as herbivore-associated molecular patterns (HAMPs), activating pattern-triggered immunity (PTI) in tobacco (Nicotiana benthamiana) and G. max. Furthermore, RPH1 stimulates jasmonic acid and ethylene biosynthesis in G. max, thereby enhancing its resistance to R. pedestris feeding. Additionally, RPH1 homologs are universally conserved across various herbivorous species, with many homologs also acting as HAMPs that trigger plant immunity. Interestingly, the remaining RPH1 putative paralogs (RPH1L2-5) serve as effectors that counteract RPH1-induced PTI, likely by disrupting the extracellular perception of RPH1. This research uncovers a HAMP whose homologs are conserved in both chewing and piercing-sucking insects. Moreover, it unveils an extracellular evasion mechanism utilized by herbivores to circumvent plant immunity using functionally differentiated paralogs.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"2812-2824"},"PeriodicalIF":6.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ubiquitin E3 ligase RZFP1 affects drought tolerance in poplar by mediating the degradation of the protein phosphatase PP2C-9. 泛素 E3 连接酶 RZFP1 通过介导蛋白磷酸酶 PP2C-9 的降解影响杨树的耐旱性。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-02 DOI: 10.1093/plphys/kiae497

Fang He, Meng-Xue Niu, Ting Wang, Jun-Lin Li, Yu-Jie Shi, Jiu-Jiu Zhao, Hao Li, Xiang Xiang, Peng Yang, Shu-Ying Wei, Tian-Tian Lin, Xiong Huang, Xinli Xia, Xue-Qin Wan

{"title":"The ubiquitin E3 ligase RZFP1 affects drought tolerance in poplar by mediating the degradation of the protein phosphatase PP2C-9.","authors":"Fang He, Meng-Xue Niu, Ting Wang, Jun-Lin Li, Yu-Jie Shi, Jiu-Jiu Zhao, Hao Li, Xiang Xiang, Peng Yang, Shu-Ying Wei, Tian-Tian Lin, Xiong Huang, Xinli Xia, Xue-Qin Wan","doi":"10.1093/plphys/kiae497","DOIUrl":"10.1093/plphys/kiae497","url":null,"abstract":"Abscisic acid (ABA) signaling has been implicated in plant responses to water deficit-induced osmotic stress. However, the underlying molecular mechanism remains unelucidated. This study identified the RING-type E3 ubiquitin ligase RING ZINC FINGER PROTEIN1 (PtrRZFP1) in poplar (Populus trichocarpa), a woody model plant. PtrRZFP1 encodes an ubiquitin E3 ligase that participates in protein ubiquitination. PtrRZFP1 mainly functions in the nucleus and endoplasmic reticulum and is activated by drought and ABA. PtrRZFP1-overexpressing transgenic poplars (35S:PtrRZFP1) showed greater tolerance to drought, whereas PtrRZFP1-knockdown lines (KD-PtrRZFP1) showed greater sensitivity to drought. Under treatment with polyethylene glycol and ABA, PtrRZFP1 promoted the production of nitric oxide and hydrogen peroxide in stomatal guard cells, ultimately enhancing stomatal closure and improving drought tolerance. Additionally, PtrRZFP1 physically interacted with the clade A Protein Phosphatase 2C protein PtrPP2C-9, a core regulator of ABA signaling, and mediated its ubiquitination and eventual degradation through the ubiquitination-26S proteasome system, indicating that PtrRZFP1 positively regulates the ABA signaling pathway. Furthermore, the PtrPP2C-9-overexpression line was insensitive to ABA and more sensitive to drought than the wild-type plants, whereas the opposite phenotype was observed in 35S:PtrRZFP1 plants. In general, PtrRZFP1 negatively regulates the stability of PtrPP2C-9 to mediate poplar drought tolerance. The results of this study provide a theoretical framework for the targeted breeding of drought-tolerant traits in perennial woody plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"2936-2955"},"PeriodicalIF":6.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vernalization or devernalization? A question about VRT2. 春化还是脱温？关于 VRT2 的问题。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-02 DOI: 10.1093/plphys/kiae440

Dechang Cao

引用次数: 0

Killing two birds with one stone: A breakthrough in transgene-free gene editing in soybean. 一石二鸟：大豆无转基因基因编辑的突破。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-02 DOI: 10.1093/plphys/kiae526

Chong Teng

引用次数: 0

A cyclical marker system enables indefinite series of oligonucleotide-directed gene editing in Chlamydomonas reinhardtii. 循环标记系统使寡核苷酸定向基因编辑在衣藻中无限系列地进行。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-02 DOI: 10.1093/plphys/kiae427

Ian L Ross, Hong Phuong Le, Sabar Budiman, Dake Xiong, Fritz Hemker, Elizabeth A Millen, Melanie Oey, Ben Hankamer

{"title":"A cyclical marker system enables indefinite series of oligonucleotide-directed gene editing in Chlamydomonas reinhardtii.","authors":"Ian L Ross, Hong Phuong Le, Sabar Budiman, Dake Xiong, Fritz Hemker, Elizabeth A Millen, Melanie Oey, Ben Hankamer","doi":"10.1093/plphys/kiae427","DOIUrl":"10.1093/plphys/kiae427","url":null,"abstract":"CRISPR/Cas9 gene editing in the model green alga Chlamydomonas reinhardtii relies on the use of selective marker genes to enrich for nonselectable target mutations. This becomes challenging when many sequential modifications are required in a single-cell line, as useful markers are limited. Here, we demonstrate a cyclical selection process which only requires a single marker gene to identify an almost infinite sequential series of CRISPR-based target gene modifications. We used the NIA1 (Nit1, NR; nitrate reductase) gene as the selectable marker in this study. In the forward stage of the cycle, a stop codon was engineered into the NIA1 gene at the CRISPR target location. Cells retaining the wild-type NIA1 gene were killed by chlorate, while NIA1 knockout mutants survived. In the reverse phase of the cycle, the stop codon engineered into the NIA1 gene during the forward phase was edited back to the wild-type sequence. Using nitrate as the sole nitrogen source, only the reverted wild-type cells survived. By using CRISPR to specifically deactivate and reactivate the NIA1 gene, a marker system was established that flipped back and forth between chlorate- and auxotrophic (nitrate)-based selection. This provided a scarless cyclical marker system that enabled an indefinite series of CRISPR edits in other, nonselectable genes. We demonstrate that this \"Sequential CRISPR via Recycling Endogenous Auxotrophic Markers (SCREAM)\" technology enables an essentially limitless series of genetic modifications to be introduced into a single-cell lineage of C. reinhardtii in a fast and efficient manner to complete complex genetic engineering.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"2330-2345"},"PeriodicalIF":6.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142047033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional diversification of Sec13 isoforms for storage protein trafficking in rice endosperm cells. Sec13 异构体在水稻胚乳细胞中运输贮藏蛋白的功能多样化

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-02 DOI: 10.1093/plphys/kiae513

Yongfei Wang, Yulong Ren, Xuan Teng, Fan Wang, Yanyu Chen, Erchao Duan, Xin Wang, Tian Pan, Binglei Zhang, Gexing Wan, Yu Zhang, Pengcheng Zhang, Xiejun Sun, Wenkun Yang, Yun Zhu, Yu Chen, Wenjie Zhao, Xiaohang Han, Cailin Lei, Shanshan Zhu, Shijia Liu, Yihua Wang, Jianmin Wan

{"title":"Functional diversification of Sec13 isoforms for storage protein trafficking in rice endosperm cells.","authors":"Yongfei Wang, Yulong Ren, Xuan Teng, Fan Wang, Yanyu Chen, Erchao Duan, Xin Wang, Tian Pan, Binglei Zhang, Gexing Wan, Yu Zhang, Pengcheng Zhang, Xiejun Sun, Wenkun Yang, Yun Zhu, Yu Chen, Wenjie Zhao, Xiaohang Han, Cailin Lei, Shanshan Zhu, Shijia Liu, Yihua Wang, Jianmin Wan","doi":"10.1093/plphys/kiae513","DOIUrl":"10.1093/plphys/kiae513","url":null,"abstract":"Coat protein complex II (COPII) vesicles play crucial roles in mediating the endoplasmic reticulum (ER) exit of newly synthesized proteins to the Golgi in eukaryotic cells. However, the molecular functions of COPII components and their functional diversifications in plant seeds remain obscure. Here, we showed that the rice (Oryza sativa) glutelin precursor accumulation12 (gpa12) mutant is defective in storage protein export from the ER, resulting in the formation of aggregated protein bodies. Map-based cloning revealed that GPA12 encodes a COPII outer layer protein, Sec13a, that mainly localizes to endoplasmic reticulum exit sites (ERES) and partially localizes to the Golgi. Biochemical experiments verified that Sec13a physically interacts with Sec31 and Sec16, and mutation in Sec13 compromises its interaction with Sec31 and Sec16, thereby affecting the membrane association of the inner complex components Sar1b and Sec23c. Apart from Sec13a, the rice genome encodes 2 other Sec13 isoforms, Sec13b and Sec13c. Notably, we observed an abnormal accumulation of globular ER structures in the sec13bc double mutant but not in the single mutants, suggesting a functional redundancy of Sec13b and Sec13c in modulating ER morphology. Taken together, our results substantiated that Sec13a plays an important role in regulating storage protein export from the ER, while Sec13b and Sec13c are required for maintaining ER morphology in rice endosperm cells. Our findings provide insights into the functional diversification of COPII components in plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"2405-2421"},"PeriodicalIF":6.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Filling the gaps on root hair development under salt stress and phosphate starvation using current evidence coupled with a meta-analysis approach. 利用现有证据和元分析方法，填补盐胁迫和磷酸盐饥饿条件下根毛发育的空白。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-02 DOI: 10.1093/plphys/kiae346

Miguel Angel Ibeas, Hernán Salinas-Grenet, Nathan R Johnson, Jorge Pérez-Díaz, Elena A Vidal, José Miguel Alvarez, José M Estevez

引用次数: 0

The white lupin trehalase gene LaTRE1 regulates cluster root formation and function under phosphorus deficiency. 白羽扇豆三卤酶基因 LaTRE1 在缺磷情况下调控丛生根的形成和功能。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-02 DOI: 10.1093/plphys/kiae290

Tianyu Xia, Xiaoqi Zhu, Yujie Zhan, Bowen Liu, Xiangxue Zhou, Qian Zhang, Weifeng Xu

{"title":"The white lupin trehalase gene LaTRE1 regulates cluster root formation and function under phosphorus deficiency.","authors":"Tianyu Xia, Xiaoqi Zhu, Yujie Zhan, Bowen Liu, Xiangxue Zhou, Qian Zhang, Weifeng Xu","doi":"10.1093/plphys/kiae290","DOIUrl":"10.1093/plphys/kiae290","url":null,"abstract":"Under phosphorus (P) deficiency, white lupin (Lupinus albus L.) forms a specialized root structure, called cluster root (CR), to improve soil exploration and nutrient acquisition. Sugar signaling is thought to play a vital role in the development of CR. Trehalose and its associated metabolites are the essential sugar signal molecules that link growth and development to carbon metabolism in plants; however, their roles in the control of CR are still unclear. Here, we investigated the function of the trehalose metabolism pathway by pharmacological and genetic manipulation of the activity of trehalase in white lupin, the only enzyme that degrades trehalose into glucose. Under P deficiency, validamycin A treatment, which inhibits trehalase, led to the accumulation of trehalose and promoted the formation of CR with enhanced organic acid production, whereas overexpression of the white lupin TREHALASE1 (LaTRE1) led to decreased trehalose levels, lateral rootlet density, and organic acid production. Transcriptomic and virus-induced gene silencing results revealed that LaTRE1 negatively regulates the formation of CRs, at least partially, by the suppression of LaLBD16, whose putative ortholog in Arabidopsis (Arabidopsis thaliana) acts downstream of ARF7- and ARF19-dependent auxin signaling in lateral root formation. Overall, our findings provide an association between the trehalose metabolism gene LaTRE1 and CR formation and function with respect to organic acid production in white lupin under P deficiency.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"2184-2198"},"PeriodicalIF":6.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11637477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141158764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outward askew endodermal cell divisions reveal INFLORESCENCE AND ROOT APICES RECEPTOR KINASE functions in division orientation. 向外歪斜的内胚层细胞分裂揭示了花期和根尖受体蛋白酶在分裂定向中的功能。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-02 DOI: 10.1093/plphys/kiae419

R M Imtiaz Karim Rony, Roya Campos, Patricio Pérez-Henríquez, Jaimie M Van Norman

{"title":"Outward askew endodermal cell divisions reveal INFLORESCENCE AND ROOT APICES RECEPTOR KINASE functions in division orientation.","authors":"R M Imtiaz Karim Rony, Roya Campos, Patricio Pérez-Henríquez, Jaimie M Van Norman","doi":"10.1093/plphys/kiae419","DOIUrl":"10.1093/plphys/kiae419","url":null,"abstract":"Oriented cell divisions establish plant tissue and organ patterning and produce different cell types; this is particularly true of the highly organized Arabidopsis (Arabidopsis thaliana) root meristem. Mutant alleles of INFLORESCENCE AND ROOT APICES RECEPTOR KINASE (IRK) exhibit excess cell divisions in the root endodermis. IRK is a transmembrane receptor kinase that localizes to the outer polar domain of these cells, suggesting that directional signal perception is necessary to repress endodermal cell division. Here, a detailed examination revealed many of the excess endodermal divisions in irk have division planes that specifically skew toward the outer lateral side. Therefore, we termed them \"outward askew\" divisions. Expression of an IRK truncation lacking the kinase domain retains polar localization and prevents outward askew divisions in irk; however, the roots exhibit excess periclinal endodermal divisions. Using cell identity markers, we show that the daughters of outward askew divisions transition from endodermal to cortical identity similar to those of periclinal divisions. These results extend the requirement for IRK beyond repression of cell division activity to include cell division plane positioning. Based on its polarity, we propose that IRK at the outer lateral endodermal cell face participates in division plane positioning to ensure normal root ground tissue patterning.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"2251-2262"},"PeriodicalIF":6.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MdIAA29-MdARF4 complex plays an important role in balancing plant height with salt and drought stress responses. MdIAA29-MdARF4 复合物在平衡植物高度与盐和干旱胁迫响应方面发挥着重要作用。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-02 DOI: 10.1093/plphys/kiae467

Yingying Lei, Cui Chen, Wenjun Chen, Hongyan Dai

{"title":"The MdIAA29-MdARF4 complex plays an important role in balancing plant height with salt and drought stress responses.","authors":"Yingying Lei, Cui Chen, Wenjun Chen, Hongyan Dai","doi":"10.1093/plphys/kiae467","DOIUrl":"10.1093/plphys/kiae467","url":null,"abstract":"Breeding dwarf apple (Malus domestica) varieties is a recent trend in agriculture because such varieties are easy to maintain and have high yields; however, dwarf apple trees generally have poor stress tolerance. Balancing apple plant height and stress response has been an important breeding goal. In this study, aux/indole-3-acetic acid 29 gene in apple (MdIAA29) overexpression lines (#1, #2, and #3) had reduced plant height by 39%, 31%, and 35%, respectively, suitable for close planting applications. Surprisingly, the dwarf MdIAA29-overexpressing lines also showed increased plant tolerance to salt and drought stresses. Further analysis showed that MdIAA29 inhibited the regulation of auxin response factor 4 (ARF4) on Gretchen Hagen 3.9 (GH3.9) gene and 9-cis-epoxycarotenoid dioxygenase 3 (NCED3) gene in apple and changed the contents of auxin and abscisic acid in different tissues, thus achieving a balance between plant height and stress tolerance. In addition, we also found that MdIAA7 enhanced the inhibitory effect of MdIAA29 on MdARF4. In brief, the MdIAA29-MdARF4 complex significantly impacts the height of apple plants and their ability to respond to salt and drought stress.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"2795-2811"},"PeriodicalIF":6.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0