Plant Physiology最新文献_第6页

Upregulation of PECTATE LYASE5 by a NAC transcription factor promotes fruit softening in apple. NAC 转录因子上调 PECTATE LYASE5 可促进苹果果实软化。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-04 DOI: 10.1093/plphys/kiae428

Qiufang Su, Huijuan Yang, Xianglu Li, Yuanwen Zhong, Yifeng Feng, Hongfei Li, Muhammad Mobeen Tahir, Zhengyang Zhao

{"title":"Upregulation of PECTATE LYASE5 by a NAC transcription factor promotes fruit softening in apple.","authors":"Qiufang Su, Huijuan Yang, Xianglu Li, Yuanwen Zhong, Yifeng Feng, Hongfei Li, Muhammad Mobeen Tahir, Zhengyang Zhao","doi":"10.1093/plphys/kiae428","DOIUrl":"10.1093/plphys/kiae428","url":null,"abstract":"Flesh firmness is a critical breeding trait that determines consumer selection, shelf life, and transportation. The genetic basis controlling firmness in apple (Malus × domestica Borkh.) remains to be fully elucidated. We aimed to decipher genetic variance for firmness at harvest and develop potential molecular markers for marker-assisted breeding. Maturity firmness for 439 F1 hybrids from a cross of \"Cripps Pink\" and \"Fuji\" was determined in 2016 and 2017. The phenotype segregated extensively, with a Gaussian distribution. In a combined bulked segregant analysis (BSA) and RNA-sequencing analysis, 84 differentially expressed genes were screened from the 10 quantitative trait loci regions. Interestingly, next-generation re-sequencing analysis revealed a Harbinger-like transposon element insertion upstream of the candidate gene PECTATE LYASE5 (MdPL5); the genotype was associated with flesh firmness at harvest. The presence of this transposon repressed MdPL5 expression and was closely linked to the extra-hard phenotype. MdPL5 was demonstrated to promote softening in apples and tomatoes. Subsequently, using the MdPL5 promoter as bait, MdNAC1-L was identified as a transcription activator that positively regulates ripening and softening in the developing fruit. We also demonstrated that MdNAC1-L could induce the up-regulation of MdPL5, MdPG1, and the ethylene-related genes MdACS1 and MdACO1. Our findings provide insight into TE-related genetic variation and the PL-mediated regulatory network for the firmness of apple fruit.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"1887-1907"},"PeriodicalIF":6.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geminivirus C4/AC4 proteins hijack cellular COAT PROTEIN COMPLEX I for chloroplast targeting and viral infections. 双子叶病毒 C4/AC4 蛋白劫持了细胞 COAT PROTEIN COMPLEX I，以实现叶绿体靶向和病毒感染。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-04 DOI: 10.1093/plphys/kiae436

Wenhao Zhao, Yinghua Ji, Yijun Zhou, Xiaofeng Wang

{"title":"Geminivirus C4/AC4 proteins hijack cellular COAT PROTEIN COMPLEX I for chloroplast targeting and viral infections.","authors":"Wenhao Zhao, Yinghua Ji, Yijun Zhou, Xiaofeng Wang","doi":"10.1093/plphys/kiae436","DOIUrl":"10.1093/plphys/kiae436","url":null,"abstract":"Geminiviruses infect numerous crops and cause extensive agricultural losses worldwide. During viral infection, geminiviral C4/AC4 proteins relocate from the plasma membrane to chloroplasts, where they inhibit the production of host defense signaling molecules. However, mechanisms whereby C4/AC4 proteins are transported to chloroplasts are unknown. We report here that tomato (Solanum lycopersicum) COAT PROTEIN COMPLEX I (COPI) components play a critical role in redistributing Tomato yellow leaf curl virus C4 protein to chloroplasts via an interaction between the C4 and β subunit of COPI. Coexpression of both proteins promotes the enrichment of C4 in chloroplasts that is blocked by a COPI inhibitor. Overexpressing or downregulating gene expression of COPI components promotes or inhibits the viral infection, respectively, suggesting a proviral role of COPI components. COPI components play similar roles in C4/AC4 transport and infections of two other geminiviruses: Beet curly top virus and East African cassava mosaic virus. Our results reveal an unconventional role of COPI components in protein trafficking to chloroplasts during geminivirus infection and suggest a broad-spectrum antiviral strategy in controlling geminivirus infections in plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"1826-1839"},"PeriodicalIF":6.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NF-YC3: The master regulator of tomato-arbuscular mycorrhizal symbiotic symphony. NF-YC3：番茄-树根菌根共生交响乐的主调节器。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-04 DOI: 10.1093/plphys/kiae435

Ritu Singh

引用次数: 0

Synergistic actions of 3 MYB transcription factors underpin blotch formation in tree peony. 三种 MYB 转录因子的协同作用是牡丹斑点形成的基础。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-04 DOI: 10.1093/plphys/kiae420

Yuting Luan, Jun Tao, Daqiu Zhao

{"title":"Synergistic actions of 3 MYB transcription factors underpin blotch formation in tree peony.","authors":"Yuting Luan, Jun Tao, Daqiu Zhao","doi":"10.1093/plphys/kiae420","DOIUrl":"10.1093/plphys/kiae420","url":null,"abstract":"Blotches in floral organs attract pollinators and promote pollination success. Tree peony (Paeonia suffruticosa Andr.) is an internationally renowned cut flower with extremely high ornamental and economic value. Blotch formation on P. suffruticosa petals is predominantly attributed to anthocyanin accumulation. However, the endogenous regulation of blotch formation in P. suffruticosa remains elusive. Here, we identified the regulatory modules governing anthocyanin-mediated blotch formation in P. suffruticosa petals, which involves the transcription factors PsMYB308, PsMYBPA2, and PsMYB21. PsMYBPA2 activated PsF3H expression to provide sufficient precursor substrate for anthocyanin biosynthesis. PsMYB21 activated both PsF3H and PsFLS expressions and promoted flavonol biosynthesis. The significantly high expression of PsMYB21 in nonblotch regions inhibited blotch formation by competing for anthocyanin biosynthesis substrates, while conversely, its low expression in the blotch region promoted blotch formation. PsMYB308 inhibited PsDFR and PsMYBPA2 expressions to directly prevent anthocyanin-mediated blotch formation. Notably, a smaller blotch area, decreased anthocyanin content, and inhibition of anthocyanin structural gene expression were observed in PsMYBPA2-silenced petals, while the opposite phenotypes were observed in PsMYB308-silenced and PsMYB21-silenced petals. Additionally, PsMYBPA2 and PsMYB308 interacted with PsbHLH1-3, and their regulatory intensity on target genes was synergistically regulated by the PsMYBPA2-PsbHLH1-3 and PsMYB308-PsbHLH1-3 complexes. PsMYB308 also competitively bound to PsbHLH1-3 with PsMYBPA2 to fine-tune the regulatory network to prevent overaccumulation of anthocyanin in blotch regions. Overall, our study uncovers a complex R2R3-MYB transcriptional regulatory network that governs anthocyanin-mediated blotch formation in P. suffruticosa petals, providing insights into the molecular mechanisms underlying blotch formation in P. suffruticosa.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"1869-1886"},"PeriodicalIF":6.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CPK10 regulates low light-induced tomato flower drop downstream of IDL6 in a calcium-dependent manner. 激酶 CPK10 以钙依赖方式调控弱光诱导的番茄落花（IDL6 的下游）。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-04 DOI: 10.1093/plphys/kiae406

Xin Fu, Ruizhen Li, Xianfeng Liu, Lina Cheng, Siqi Ge, Sai Wang, Yue Cai, Tong Zhang, Chun-Lin Shi, Sida Meng, Changhua Tan, Cai-Zhong Jiang, Tianlai Li, Mingfang Qi, Tao Xu

{"title":"CPK10 regulates low light-induced tomato flower drop downstream of IDL6 in a calcium-dependent manner.","authors":"Xin Fu, Ruizhen Li, Xianfeng Liu, Lina Cheng, Siqi Ge, Sai Wang, Yue Cai, Tong Zhang, Chun-Lin Shi, Sida Meng, Changhua Tan, Cai-Zhong Jiang, Tianlai Li, Mingfang Qi, Tao Xu","doi":"10.1093/plphys/kiae406","DOIUrl":"10.1093/plphys/kiae406","url":null,"abstract":"Flower drop is a major cause for yield loss in many crops. Previously, we found that the tomato (Solanum lycopersicum) INFLORESCENCE DEFICIENT IN ABSCISSION-Like (SlIDL6) gene contributes to flower drop induced by low light. However, the molecular mechanisms by which SlIDL6 acts as a signal to regulate low light-induced abscission remain unclear. In this study, SlIDL6 was found to elevate cytosolic Ca2+ concentrations ([Ca2+]cyt) in the abscission zone (AZ), which was required for SlIDL6-induced flower drop under low light. We further identified that 1 calcium-dependent protein kinase gene, SlCPK10, was highly expressed in the AZ and upregulated by SlIDL6-triggered [Ca2+]cyt. Overexpression and knockout of SlCPK10 in tomato resulted in accelerated and delayed abscission, respectively. Genetic evidence further indicated that knockout of SlCPK10 significantly impaired the function of SlIDL6 in accelerating abscission. Furthermore, Ser-371 phosphorylation in SlCPK10 dependent on SlIDL6 was necessary and sufficient for its function in regulating flower drop, probably by stabilizing the SlCPK10 proteins. Taken together, our findings reveal that SlCPK10, as a downstream component of the IDL6 signaling pathway, regulates flower drop in tomato under low-light stress.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"2014-2029"},"PeriodicalIF":6.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142110848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complexity of volatile terpene biosynthesis in roses: Particular insights into β-citronellol production. 玫瑰中挥发性萜烯生物合成的复杂性：对 β-Citronellol 生产的特殊见解。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-04 DOI: 10.1093/plphys/kiae444

Hongjie Li, Yueqing Li, Huijun Yan, Tingting Bao, Xiaotong Shan, Jean-Claude Caissard, Liangsheng Zhang, Huiyi Fang, Xue Bai, Jia Zhang, Zhaoxuan Wang, Min Wang, Qian Guan, Ming Cai, Guogui Ning, Xiujuan Jia, Benoît Boachon, Sylvie Baudino, Xiang Gao

{"title":"The complexity of volatile terpene biosynthesis in roses: Particular insights into β-citronellol production.","authors":"Hongjie Li, Yueqing Li, Huijun Yan, Tingting Bao, Xiaotong Shan, Jean-Claude Caissard, Liangsheng Zhang, Huiyi Fang, Xue Bai, Jia Zhang, Zhaoxuan Wang, Min Wang, Qian Guan, Ming Cai, Guogui Ning, Xiujuan Jia, Benoît Boachon, Sylvie Baudino, Xiang Gao","doi":"10.1093/plphys/kiae444","DOIUrl":"10.1093/plphys/kiae444","url":null,"abstract":"The fascinating scent of rose (Rosa genus) flowers has captivated human senses for centuries, making them one of the most popular and widely used floral fragrances. Despite much progress over the last decade, many biochemical pathways responsible for rose scents remain unclear. We analyzed the floral scent compositions from various rose varieties and selected the modern cultivar Rosa hybrida \"Double Delight\" as a model system to unravel the formation of rose dominant volatile terpenes, which contribute substantially to the rose fragrance. Key genes involved in rose terpene biosynthesis were functionally characterized. Cytosolic geranyl diphosphate (GPP) generated by geranyl/farnesyl diphosphate synthase (G/FPPS1) catalysis played a pivotal role in rose scent production, and terpene synthases in roses play an important role in the formation of most volatile terpenes, but not for geraniol, citral, or β-citronellol. Subsequently, a series of enzymes, including geraniol dehydrogenase, geranial reductase, 12-oxophytodienoate reductase, and citronellal reductase, were characterized as involved in the transformation of geraniol to β-citronellol in roses through three successive steps. Interestingly, the β-citronellol biosynthesis pathway appears to be conserved in other horticultural plants like Lagerstroemia caudata and Paeonia lactiflora. Our findings provide valuable insights into the biosynthesis of rose volatile terpenoid compounds and offer essential gene resources for future breeding and molecular modification efforts.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"1908-1922"},"PeriodicalIF":6.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal and spatial frameworks supporting plant responses to vegetation proximity. 支持植物对植被邻近性做出反应的时空框架。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-04 DOI: 10.1093/plphys/kiae417

Pedro Pastor-Andreu, Jordi Moreno-Romero, Mikel Urdin-Bravo, Julia Palau-Rodriguez, Sandi Paulisic, Elizabeth Kastanaki, Vicente Vives-Peris, Aurelio Gomez-Cadenas, Anna Esteve-Codina, Beatriz Martín-Mur, Antía Rodríguez-Villalón, Jaume F Martínez-García

{"title":"Temporal and spatial frameworks supporting plant responses to vegetation proximity.","authors":"Pedro Pastor-Andreu, Jordi Moreno-Romero, Mikel Urdin-Bravo, Julia Palau-Rodriguez, Sandi Paulisic, Elizabeth Kastanaki, Vicente Vives-Peris, Aurelio Gomez-Cadenas, Anna Esteve-Codina, Beatriz Martín-Mur, Antía Rodríguez-Villalón, Jaume F Martínez-García","doi":"10.1093/plphys/kiae417","DOIUrl":"10.1093/plphys/kiae417","url":null,"abstract":"After the perception of vegetation proximity by phytochrome photoreceptors, shade-avoider plants initiate a set of responses known as the shade avoidance syndrome (SAS). Shade perception by the phytochrome B (phyB) photoreceptor unleashes the PHYTOCHROME INTERACTING FACTORs and initiates SAS responses. In Arabidopsis (Arabidopsis thaliana) seedlings, shade perception involves rapid and massive changes in gene expression, increases auxin production, and promotes hypocotyl elongation. Other components, such as phyA and ELONGATED HYPOCOTYL 5, also participate in the shade regulation of the hypocotyl elongation response by repressing it. However, why and how so many regulators with either positive or negative activities modulate the same response remains unclear. Our physiological, genetic, cellular, and transcriptomic analyses showed that (i) these components are organized into 2 main branches or modules and (ii) the connection between them is dynamic and changes with the time of shade exposure. We propose a model for the regulation of shade-induced hypocotyl elongation in which the temporal and spatial functional importance of the various SAS regulators analyzed here helps to explain the coexistence of differentiated regulatory branches with overlapping activities.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"2048-2063"},"PeriodicalIF":6.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11531833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allele-specific expression of AP2-like ABA repressor 1 regulates iron uptake by modulating rhizosphere pH in apple. 类似 AP2 的 ABA 抑制因子 1 的等位基因特异性表达通过调节苹果根瘤层的 pH 值来调控铁的吸收。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-04 DOI: 10.1093/plphys/kiae452

Huaying Ma, Mengmeng Fu, Zhen Xu, Zicheng Chu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu

{"title":"Allele-specific expression of AP2-like ABA repressor 1 regulates iron uptake by modulating rhizosphere pH in apple.","authors":"Huaying Ma, Mengmeng Fu, Zhen Xu, Zicheng Chu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu","doi":"10.1093/plphys/kiae452","DOIUrl":"10.1093/plphys/kiae452","url":null,"abstract":"Genetic variation within a species can result in allelic expression for natural selection or breeding efforts. Here, we identified an iron (Fe) deficiency-inducible gene, AP2-like ABA repressor 1 (MdABR1), in apple (Malus domestica). MdABR1 exhibited differential expression at the allelic level (MdABR131A and MdABR131G) in response to Fe deficiency. The W-box insertion in the promoter of MdABR131A is essential for its induced expression and its positive role under Fe deficiency stress. MdABR1 binds to the promoter of basic helix-loop-helix 105 (MdbHLH105), participating in the Fe deficiency response, and activates its transcription. MdABR131A exerts a more pronounced transcriptional activation effect on MdbHLH105. Suppression of MdABR1 expression leads to reduced rhizosphere acidification in apple, and MdABR131A exhibits allelic expression under Fe deficiency stress, which is substantially upregulated and then activates the expression of MdbHLH105, promoting the accumulation of plasma membrane proton ATPase 8 (MdAHA8) transcripts in response to proton extrusion, thereby promoting rhizosphere acidification. Therefore, variation in the ABR1 alleles results in variable gene expression and enables apple plants to exhibit a wider tolerance capability and Fe deficiency response. These findings also shed light on the molecular mechanisms of allele-specific expression in woody plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"2121-2136"},"PeriodicalIF":6.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142086196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cytoskeleton controls the dynamics of plasma membrane proteins and facilitates their endocytosis in plants. 在植物体内，细胞骨架控制着质膜蛋白的动态，并促进它们的内吞。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-04 DOI: 10.1093/plphys/kiae403

Pengyun Luo, Xinxiu Zuo, Yufen Bu, Hongping Qian, Changwen Xu, Shihui Niu, Jinxing Lin, Yaning Cui

{"title":"The cytoskeleton controls the dynamics of plasma membrane proteins and facilitates their endocytosis in plants.","authors":"Pengyun Luo, Xinxiu Zuo, Yufen Bu, Hongping Qian, Changwen Xu, Shihui Niu, Jinxing Lin, Yaning Cui","doi":"10.1093/plphys/kiae403","DOIUrl":"10.1093/plphys/kiae403","url":null,"abstract":"Plasma membranes (PMs) are highly dynamic structures where lipids and proteins can theoretically diffuse freely. However, reports indicate that PM proteins do not freely diffuse within their planes but are constrained by cytoskeleton networks, though the mechanisms for how the cytoskeleton restricts lateral diffusion of plant PM proteins are unclear. Through single-molecule tracking, we investigated the dynamics of 6 Arabidopsis (Arabidopsis thaliana) PM proteins with diverse structures and found distinctions in sizes and dynamics among these proteins. Moreover, we showed that the cytoskeleton, particularly microtubules, limits the diffusion of PM proteins, including transmembrane and membrane-anchoring proteins. Interestingly, the microfilament skeleton regulates intracellular transport of endocytic cargo. Therefore, these findings indicate that the cytoskeleton controls signal transduction by limiting diffusion of PM proteins in specific membrane compartments and participating in transport of internalized cargo vesicles, thus actively regulating plant signal transduction.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"1813-1825"},"PeriodicalIF":6.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141793021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wheat MYOSIN-RESEMBLING CHLOROPLAST PROTEIN controls B-type starch granule initiation timing during endosperm development. 小麦肌球蛋白-重组壳蛋白控制胚乳发育过程中 B 型淀粉粒的启动时间。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-04 DOI: 10.1093/plphys/kiae429

Jiawen Chen, Yi Chen, Alexander Watson-Lazowski, Erica Hawkins, J Elaine Barclay, Brendan Fahy, Robin Denley Bowers, Kendall Corbin, Frederick J Warren, Andreas Blennow, Cristobal Uauy, David Seung

{"title":"Wheat MYOSIN-RESEMBLING CHLOROPLAST PROTEIN controls B-type starch granule initiation timing during endosperm development.","authors":"Jiawen Chen, Yi Chen, Alexander Watson-Lazowski, Erica Hawkins, J Elaine Barclay, Brendan Fahy, Robin Denley Bowers, Kendall Corbin, Frederick J Warren, Andreas Blennow, Cristobal Uauy, David Seung","doi":"10.1093/plphys/kiae429","DOIUrl":"10.1093/plphys/kiae429","url":null,"abstract":"Molecular factors that contribute to the diverse spatial and temporal patterns of starch granule initiation between species and organs are poorly understood. Wheat (Triticum sp.) endosperm contains both large A-type granules initiated during early grain development and small B-type granules that initiate about 10 to 15 days later. Here, we identify that the MYOSIN-RESEMBLING CHLOROPLAST PROTEIN (MRC) is required for the correct timing of B-type granule initiation in wheat endosperm during grain development. MRC is expressed in the endosperm exclusively in early grain development, before B-type granule initiation. We isolated three independent TILLING mutants of tetraploid wheat (Triticum turgidum cv. 'Kronos') with premature stop or missense mutations in the A-genome homeolog, which we showed to be the only active homeolog in tetraploid wheat due to a disruption of the B-genome homeolog. The mrc mutants had significantly smaller A-type granules and a higher relative volume of B-type granules in the endosperm than the wild type. Whereas B-type granules initiated 15 to 20 days post-anthesis (dpa) in the wild type, they appeared as early as 10 dpa in the mrc-1 mutant. These results suggest a temporal role for MRC in repressing B-type granule initiation, providing insight into how the distinct biochemical mechanisms that control A- and B-type granule initiation are regulated. This role of MRC in the wheat endosperm is distinct from the previously described role of Arabidopsis (Arabidopsis thaliana) MRC in promoting granule initiation in leaves, providing an example of functional diversification among granule initiation proteins.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"1980-1996"},"PeriodicalIF":6.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11531834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0