Plant Physiology最新文献_第6页

Uneven gains: the effects of elevated CO2 and temperatures on photosynthetic response of boreal tree seedlings. 收益不均：CO2和温度升高对北方乔木幼苗光合反应的影响。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf442

Alice Gauthey

引用次数: 0

eRUBY rice: Co-expression of a feedback-insensitive TyrA arogenate dehydrogenase with RUBY enhances endosperm betalain levels. 红宝石：反馈不敏感的TyrA - argenate脱氢酶与RUBY的共表达提高了胚乳β素水平。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf416

Jiantao Tan,Yaxi Wang,Zhansheng Lin,Nan Chai,Yang Xue,Letian Chen,Yao-Guang Liu,Qinlong Zhu

引用次数: 0

ISOPENTENYL TRANSFERASE3 activation triggers root ammonium hypersensitivity via the cytokinin-ARR10/ARR12-CAP1 signaling pathway ISOPENTENYL TRANSFERASE3激活通过细胞分裂素- arr10 /ARR12-CAP1信号通路触发根铵超敏反应

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf462

Ting-Ting Li, Lei Wu, Meng Wang, Herbert J Kronzucker, Yunqi Liu, Weiming Shi, Dong-Wei Di

{"title":"ISOPENTENYL TRANSFERASE3 activation triggers root ammonium hypersensitivity via the cytokinin-ARR10/ARR12-CAP1 signaling pathway","authors":"Ting-Ting Li, Lei Wu, Meng Wang, Herbert J Kronzucker, Yunqi Liu, Weiming Shi, Dong-Wei Di","doi":"10.1093/plphys/kiaf462","DOIUrl":"https://doi.org/10.1093/plphys/kiaf462","url":null,"abstract":"High ammonium (NH₄⁺) levels inhibit primary root (PR) elongation in plants, but the underlying regulatory mechanisms remain poorly understood. In this study, we screened the Arabidopsis (Arabidopsis thaliana) PSKI015 activation-tagged mutant library and identified a dominant mutant, named Ammonium Sensitive 3D (amos3D), which shows increased sensitivity to high NH₄⁺ in terms of PR elongation. Gene cloning revealed that amos3D overexpresses IPT3, a gene involved in cytokinin biosynthesis. Pharmacological and genetic analyses revealed that the PR sensitivity to high NH₄⁺ in amos3D is due to elevated levels of the active cytokinins iP and tZ. Furthermore, we identified the type-B ARRs ARR10 and ARR12 as key transcription factors in the cytokinin-mediated inhibition of PR elongation under high-NH₄⁺ stress. Using CUT&RUN (Cleavage Under Targets & Release Using Nuclease), yeast one-hybrid, and dual-luciferase assays, we showed that ARR10 and ARR12 directly bind to the promoter of CAP1, a tonoplast-localized kinase, repressing its transcription. This repression reduces NH₄⁺ transport from the cytosol to the vacuole, leading to increased Gln/Glu ratios and enhanced NH₄⁺ toxicity. Collectively, our identification of AMOS3 as a key inhibitor of PR growth under high NH₄⁺ through the cytokinin-dependent ARR10/ARR12–CAP1 pathway not only reveals an NH₄⁺-sensing mechanism but also offers promising agronomic potential for optimizing root architecture and improving nitrogen-acquisition efficiency in crops under ammonium-based fertilization systems.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"105 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Base editing of BnaFAD2 fine-tunes oleic acid content in allotetraploid rapeseed without compromising yield. 碱基编辑BnaFAD2可以在不影响产量的情况下微调异源四倍体油菜的油酸含量。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf467

Jingzhen Wang,Qing Dong,Yan Li,Limin Hu,Chenxi Jia,Huailin Li,Hanzi He,Chuchuan Fan

{"title":"Base editing of BnaFAD2 fine-tunes oleic acid content in allotetraploid rapeseed without compromising yield.","authors":"Jingzhen Wang,Qing Dong,Yan Li,Limin Hu,Chenxi Jia,Huailin Li,Hanzi He,Chuchuan Fan","doi":"10.1093/plphys/kiaf467","DOIUrl":"https://doi.org/10.1093/plphys/kiaf467","url":null,"abstract":"Rapeseed (Brassica napus L.) is a major global oil crop, with its nutritional and functional value largely determined by the degree of fatty acid desaturation in seed oil. High-oleic rapeseed is primarily developed through loss-of-function mutations in the fatty acid desaturase 2 gene (BnaFAD2). However, BnaFAD2 is dose-sensitive, and complete gene inactivation often leads to negative phenotypes. To mitigate negative effects on plant growth, we generated BnaFAD2 amino acid variants using base editing. Here, we successfully developed BnSTEME, a dual-base editing system optimized for allotetraploid rapeseed, which targeted conserved domains of BnaFAD2.A5 and BnaFAD2.C5 via 15 sgRNAs. This system enabled simultaneous C-to-T and A-to-G conversions with a base-editing efficiency of 1.29%. BnSTEME together with our previously established cytosine base editor BnA3A1-PBE induced a total of 29 missense mutations in BnaFAD2, including 26 variant alleles not previously reported. By oleic acid content screening, we identified two key mutations-S229F (a strong allele) and D230N (a weak allele)-that significantly increased oleic acid content, yielding rapeseed lines with oleic acid content ranging from 71.08% to 84.22%. Field trials for the base-edited line with the highest oleic acid PST-Z3-15-56-1 (aaS229FccD230N, 84.22% C18:1) demonstrated agronomic stability without yield penalty, which outperformed the knockout line CR-aacc. This study establishes base editing as a powerful tool for fine-tuning dosage-sensitive traits in polyploid crops and provides high-oleic rapeseed germplasm for sustainable oilseed breeding.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"11 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chloroplast budding mediates β-carotene transport for early stage astaxanthin hyperaccumulation in microalgae. 微藻叶绿体出芽介导β-胡萝卜素运输，促进早期虾青素超积累。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf423

Haiyan Ma, Zhaokun Wang, Feng Ge, Cheng-Cai Zhang

引用次数: 0

Elevated atmospheric CO2-induced reprogramming leads to decreased seed protein and nutritional quality in forest trees. 大气co2升高引起的重编程导致森林树木种子蛋白质和营养品质下降。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf463

Barbara Karpinska,Rosa Sanchez-Lucas,Andrew Plackett,A Rob MacKenzie,Christine Helen Foyer

{"title":"Elevated atmospheric CO2-induced reprogramming leads to decreased seed protein and nutritional quality in forest trees.","authors":"Barbara Karpinska,Rosa Sanchez-Lucas,Andrew Plackett,A Rob MacKenzie,Christine Helen Foyer","doi":"10.1093/plphys/kiaf463","DOIUrl":"https://doi.org/10.1093/plphys/kiaf463","url":null,"abstract":"While the global increases in atmospheric CO2 levels have had a beneficial effect on plant growth, the negative impacts of this CO2 fertilisation effect on seed quality are often overlooked. Using data from acorns produced by mature oak (Quercus robur) trees in the eighth year of elevated CO2 (eCO₂), we present evidence of negative consequences for seed quality. The acorns produced by the near-200-year-old oak trees under eCO₂ at the Free Air Carbon dioxide (FACE) facility at the Birmingham Institute for Forest Research (BIFoR) had higher phytate levels but a decreased protein content. Quantitative label-free proteomics identified 335 proteins in all acorns, but 9 proteins were undetectable in acorns produced under eCO2 compared to ambient air (aCO2), and 1 protein was uniquely detected in the eCO₂ acorns. Further subsets of proteins were identified with either higher or lower abundance in eCO₂ than aCO₂ acorns. Proteins that were more abundant in the acorns produced under eCO₂ include allene oxide cyclase and phosphomannomutase. RNA-seq analysis revealed that 154 transcripts were more abundant in the eCO2 acorns compared to those grown under aCO2, while 54 were much less abundant. Transcripts encoding several transcription factors and phytohormone signalling proteins, as well as trehalose 6-phosphate phosphatase were increased in eCO2 acorns. Taken together, these findings demonstrate that the transcriptome and proteome profiles of acorns produced under eCO2 are significantly changed compared to those produced in aCO2, with important implications for seed metabolism, particularly those underpinning the observed changes in seed protein and phytate levels.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"31 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promoter editing of LpNOL generates stay-green perennial ryegrass with improved forage quality and heat tolerance. 通过对LpNOL启动子的编辑，产生了保持绿色的多年生黑麦草，改善了牧草品质和耐热性。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf402

Huadong Yang,Jiaming Yao,Huanhuan Hao,Li Jiang,Jieqin Li,Yingjun Chi,Jing Zhang,Bin Xu

引用次数: 0

The transcription factor bZIP44 regulates α-linolenic acid accumulation by activating FATTY ACID DESATURASE3 in tree peony 转录因子bZIP44通过激活脂肪酸DESATURASE3调控牡丹α-亚麻酸积累

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-29 DOI: 10.1093/plphys/kiaf430

Ling Zhang, Qiafan Su, Dandan Yin, Hongfeng Huang, Liangsheng Wang, Shanshan Li

{"title":"The transcription factor bZIP44 regulates α-linolenic acid accumulation by activating FATTY ACID DESATURASE3 in tree peony","authors":"Ling Zhang, Qiafan Su, Dandan Yin, Hongfeng Huang, Liangsheng Wang, Shanshan Li","doi":"10.1093/plphys/kiaf430","DOIUrl":"https://doi.org/10.1093/plphys/kiaf430","url":null,"abstract":"α-Linolenic acid (ALA) is an essential fatty acid crucial for improving dietary nutrition in humans. Since ALA can only be obtained from dietary sources, developing ALA-rich biological resources is imperative. The seed oil of tree peony (Paeonia × suffruticosa), a promising woody oil crop, is particularly rich in ALA. In this study, we identified PsbZIP44, a basic leucine zipper transcription factor (bZIP) in tree peony, as a key regulator of ALA accumulation in seeds. Heterologous expression of PsbZIP44 in Arabidopsis (Arabidopsis thaliana) promoted ALA accumulation and transient silencing in tree peony seeds decreased ALA levels. Additionally, we found that another bZIP transcription factor, PsbZIP9, negatively regulates ALA biosynthesis by inhibiting PsbZIP44 activity during the late stages of seed development. Based on these findings, we propose a model for FATTY ACID DESATURASE3 (PsFAD3) regulation in tree peony seeds, in which PsbZIP44 activates PsFAD3 during early seed development to promote ALA biosynthesis, while PsbZIP9 suppresses PsbZIP44 activity in later stages, limiting ALA accumulation. This study highlights the synergistic role of transcription factors in seed development, reveals the regulatory mechanisms of PsFAD3, and enhances our understanding of ALA biosynthesis. These findings provide a foundation for improving the quality of tree peony seed oil.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"92 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine-tuning OsTCP19 expression offers broad adaptation scenarios for nitrogen-use efficiency improvement in rice 调控OsTCP19表达为提高水稻氮素利用效率提供了广泛的适应方案

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-29 DOI: 10.1093/plphys/kiaf412

Yongqiang Liu, Weiwei Li, Xiaohan Wang, Yaping Li, Wenjun Zhu, Zhuo Chen, Xinyu Liu, Xiujie Liu, Wei Wang, Chenxiao Xue, Shouyun Cao, Caixia Gao, Peitao Lü, Xianzhi Xie, Bin Hu, Chengcai Chu

{"title":"Fine-tuning OsTCP19 expression offers broad adaptation scenarios for nitrogen-use efficiency improvement in rice","authors":"Yongqiang Liu, Weiwei Li, Xiaohan Wang, Yaping Li, Wenjun Zhu, Zhuo Chen, Xinyu Liu, Xiujie Liu, Wei Wang, Chenxiao Xue, Shouyun Cao, Caixia Gao, Peitao Lü, Xianzhi Xie, Bin Hu, Chengcai Chu","doi":"10.1093/plphys/kiaf412","DOIUrl":"https://doi.org/10.1093/plphys/kiaf412","url":null,"abstract":"Inconsistent nitrogen application rates across global croplands necessitate the adaptation of different genetic alleles to optimize nitrogen-use efficiency (NUE) in agriculture. In rice (Oryza sativa L.), different TEOSINTE BRANCHED 1, CYCLOIDEA AND PROLIFERATING CELL FACTOR 19 (OsTCP19) alleles contribute to the geographical adaptation to soil fertility. The nitrogen-sensitive allele OsTCP19-H is predominantly found in low-nitrogen regions due to its superior tillering and yield accumulation capabilities. Conversely, the nitrogen-insensitive allele OsTCP19-L is more common in nitrogen-sufficient regions, though the precise factors affecting this distribution remain unknown. Here, we report that high nitrogen levels lead to lodging in a OsTCP19-dependent manner. Overexpression of this gene modifies plant architecture, enhancing lodging resistance in rice. Importantly, fine-tuning OsTCP19 expression can confer lodging resistance without yield penalty. This is particularly important as it alleviates yield loss from lodging under high-nitrogen conditions and, intriguingly, can result in a substantial increase in plot yield when combined with dense planting strategies. Furthermore, the distinct nitrogen sensitivity of different OsTCP19 alleles allows for substantial improvement in grain yield and NUE under certain nitrogen conditions. Thus, our findings suggest that genetic manipulation of a single gene, OsTCP19, could allow flexible adaptation to diverse planting scenarios, maximizing genetic benefits based on local nitrogen availability.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"65 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant stem cells maintain genome stability by limiting protein synthesis 植物干细胞通过限制蛋白质合成来维持基因组的稳定性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-29 DOI: 10.1093/plphys/kiaf433

Meng Su, Xiaoya Qu, Haijun Wu, Zhong Zhao, Cheng-Wu Liu

引用次数: 0