Dylan J Ziegler, Deirdre Khan, Jenna L Kalichuk, Michael G Becker, Asher Pasha, Nicholas J Provart, Mark F Belmonte
{"title":"甘蓝型油菜种子基因表达格局揭示了亚基因组在空间和时间上的偏倚","authors":"Dylan J Ziegler, Deirdre Khan, Jenna L Kalichuk, Michael G Becker, Asher Pasha, Nicholas J Provart, Mark F Belmonte","doi":"10.1093/plphys/kiaf283","DOIUrl":null,"url":null,"abstract":"Brassica napus (canola; AnAnCnCn) contains both complete diploid genomes from its progenitors B. rapa (An) and B. oleracea (Cn). Despite growing knowledge of the gene expression landscape of the B. napus seed, little is known about subgenome bias underpinning the development of specific cells and tissues across the seed lifecycle. Here, we present a large-scale transcriptome atlas of the B. napus seed, including both the maternal seed coat and filial embryo and endosperm subregions. We report on extensive, global Cn subgenome bias throughout development and use homoeologous gene pairs to describe how subgenomic bias differs across subregions. We find that subgenome bias is most prominent during early development and that the maternal subregions experience far more asymmetric transcript accumulation in favour of the Cn subgenome. In particular, the unexpectedly distinct transcriptome profile of the chalazal pole indicates the unique developmental processes involved within the chalaza. Further, we report that genes integral to seed storage comprise a large portion of the transcriptome of mature seeds, especially within the embryo, and that gene pairs previously documented to be instrumental in seed development exhibit low transcriptional bias. This work represents an important synthesis of polyploid transcriptomics in seed biology and provides a comprehensive overview of the B. napus gene expression landscape in both space and time.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"143 1","pages":""},"PeriodicalIF":6.5000,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Gene expression landscape of the Brassica napus seed reveals subgenome bias in both space and time\",\"authors\":\"Dylan J Ziegler, Deirdre Khan, Jenna L Kalichuk, Michael G Becker, Asher Pasha, Nicholas J Provart, Mark F Belmonte\",\"doi\":\"10.1093/plphys/kiaf283\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Brassica napus (canola; AnAnCnCn) contains both complete diploid genomes from its progenitors B. rapa (An) and B. oleracea (Cn). Despite growing knowledge of the gene expression landscape of the B. napus seed, little is known about subgenome bias underpinning the development of specific cells and tissues across the seed lifecycle. Here, we present a large-scale transcriptome atlas of the B. napus seed, including both the maternal seed coat and filial embryo and endosperm subregions. We report on extensive, global Cn subgenome bias throughout development and use homoeologous gene pairs to describe how subgenomic bias differs across subregions. We find that subgenome bias is most prominent during early development and that the maternal subregions experience far more asymmetric transcript accumulation in favour of the Cn subgenome. In particular, the unexpectedly distinct transcriptome profile of the chalazal pole indicates the unique developmental processes involved within the chalaza. Further, we report that genes integral to seed storage comprise a large portion of the transcriptome of mature seeds, especially within the embryo, and that gene pairs previously documented to be instrumental in seed development exhibit low transcriptional bias. This work represents an important synthesis of polyploid transcriptomics in seed biology and provides a comprehensive overview of the B. napus gene expression landscape in both space and time.\",\"PeriodicalId\":20101,\"journal\":{\"name\":\"Plant Physiology\",\"volume\":\"143 1\",\"pages\":\"\"},\"PeriodicalIF\":6.5000,\"publicationDate\":\"2025-06-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/plphys/kiaf283\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/plphys/kiaf283","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Gene expression landscape of the Brassica napus seed reveals subgenome bias in both space and time
Brassica napus (canola; AnAnCnCn) contains both complete diploid genomes from its progenitors B. rapa (An) and B. oleracea (Cn). Despite growing knowledge of the gene expression landscape of the B. napus seed, little is known about subgenome bias underpinning the development of specific cells and tissues across the seed lifecycle. Here, we present a large-scale transcriptome atlas of the B. napus seed, including both the maternal seed coat and filial embryo and endosperm subregions. We report on extensive, global Cn subgenome bias throughout development and use homoeologous gene pairs to describe how subgenomic bias differs across subregions. We find that subgenome bias is most prominent during early development and that the maternal subregions experience far more asymmetric transcript accumulation in favour of the Cn subgenome. In particular, the unexpectedly distinct transcriptome profile of the chalazal pole indicates the unique developmental processes involved within the chalaza. Further, we report that genes integral to seed storage comprise a large portion of the transcriptome of mature seeds, especially within the embryo, and that gene pairs previously documented to be instrumental in seed development exhibit low transcriptional bias. This work represents an important synthesis of polyploid transcriptomics in seed biology and provides a comprehensive overview of the B. napus gene expression landscape in both space and time.
期刊介绍:
Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research.
As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.