Plant Physiology最新文献

How slender bamboo reacts to gravitational and external mechanical stimuli: responses and mechanisms. 细长的竹子对重力和外部机械刺激的反应：反应和机制。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-13 DOI: 10.1093/plphys/kiaf488

Yu Luan,Yichen Yang,Menghong Jiang,Shanying Li,Huanrong Liu,Fengbo Sun,Xinxin Ma,Hu Miao,Changhua Fang

{"title":"How slender bamboo reacts to gravitational and external mechanical stimuli: responses and mechanisms.","authors":"Yu Luan,Yichen Yang,Menghong Jiang,Shanying Li,Huanrong Liu,Fengbo Sun,Xinxin Ma,Hu Miao,Changhua Fang","doi":"10.1093/plphys/kiaf488","DOIUrl":"https://doi.org/10.1093/plphys/kiaf488","url":null,"abstract":"Bamboo is renowned for its rapid growth rate and striking slenderness. Despite the challenges to mechanical stability posed by its slender structure, bamboo demonstrates an exceptional ability to resist external forces. How does it accomplish this? In this study, we focused on upright plants of Moso bamboo (Phyllostachys edulis), as well as those that were bending naturally or inclined artificially, exploring their responses to gravitational and mechanical stimuli. Artificially inclined bamboo showed remarkable control and reorientation of posture through the independent sensing of local angles at each internode. Strain gauge tests revealed that bamboo possesses growth stress, forming an intrinsic support system. Upright bamboo plants displayed longitudinal tensile stresses, enhancing their stability amidst disturbances. Non-upright bamboo plants exhibited a pronounced asymmetrical stress distribution, with higher tensile stress on one side, which is crucial for maintaining a slanted posture. We detected variability in stress on the opposite side, ranging from low-level tensile stress to compressive stress, demonstrating bamboo's dynamic response to stimuli. Moreover, growth stress was detected at the cellular level, providing a foundation for investigating the origin and generation of growth stress in bamboo. Multiscale studies revealed adaptations across the macroscopic appearance, microscopic structure, and physiological functions of bamboo in response to gravitational and external mechanical stimuli, which are closely linked to mechanical responses, thereby ensuring efficient growth and stability.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"116 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A network of RS splicing regulatory proteins controls light-dependent splicing and seedling development RS剪接调节蛋白网络控制光依赖性剪接和幼苗发育

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-09 DOI: 10.1093/plphys/kiaf482

Jennifer Saile, Hannah Walter, Moritz Denecke, Patrick Lederer, Laura Schütz, Andreas Hiltbrunner, Katharina Lepp, Sofia Lobato-Gil, Petra Beli, Andreas Wachter

{"title":"A network of RS splicing regulatory proteins controls light-dependent splicing and seedling development","authors":"Jennifer Saile, Hannah Walter, Moritz Denecke, Patrick Lederer, Laura Schütz, Andreas Hiltbrunner, Katharina Lepp, Sofia Lobato-Gil, Petra Beli, Andreas Wachter","doi":"10.1093/plphys/kiaf482","DOIUrl":"https://doi.org/10.1093/plphys/kiaf482","url":null,"abstract":"The light-induced change from skoto- to photomorphogenesis is a key switch in plant development that requires global transcriptome reprogramming. Earlier studies in Arabidopsis (Arabidopsis thaliana) and other plant species have revealed the eminent role of alternative precursor mRNA splicing (AS), which allows fine-tuning the expression of numerous genes including light signalling and photosynthesis-related components in response to the ambient light conditions. Starting from the previous finding that AS changes induced by either light or metabolic signals are linked to phospho-signalling, we applied phospho-proteomics to identify proteins that undergo rapid changes in their phosphorylation status upon exposing etiolated seedlings to either light or sucrose. This approach revealed hyperphosphorylation of RS41, a member of the RS subfamily of serine/arginine-rich (SR) proteins. To study the function of the four RS genes RS31a, RS31, RS40, and RS41, we generated a comprehensive set of single and higher order mutants. A complete loss of RS function in the quadruple mutant caused sterility. Moreover, the important role of the RS proteins in seedling photomorphogenesis was demonstrated, with both redundant and specific functions in the regulation of hypocotyl elongation and cotyledon opening. We further identified the critical contribution of the RS proteins to light-dependent AS, being part of an intricate network of splicing regulatory components. Our study provides insight into the complex network of RNA-binding proteins that allow balancing light-responsive splicing and development in Arabidopsis seedlings.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"17 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145255000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jasmonic acid signaling inhibits axillary bud development under drought stress via the CmMYC2-CmBRC1b module. 茉莉酸信号通过CmMYC2-CmBRC1b模块抑制干旱胁迫下腋芽发育。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-09 DOI: 10.1093/plphys/kiaf505

Jinyu Jin,Yuqing Zhu,Song Li,Xiaojuan Xing,Yaoyao Huang,Yun Tang,Ye Liu,Jiafu Jiang,Sumei Chen,Fadi Chen,Weimin Fang,Aiping Song

{"title":"Jasmonic acid signaling inhibits axillary bud development under drought stress via the CmMYC2-CmBRC1b module.","authors":"Jinyu Jin,Yuqing Zhu,Song Li,Xiaojuan Xing,Yaoyao Huang,Yun Tang,Ye Liu,Jiafu Jiang,Sumei Chen,Fadi Chen,Weimin Fang,Aiping Song","doi":"10.1093/plphys/kiaf505","DOIUrl":"https://doi.org/10.1093/plphys/kiaf505","url":null,"abstract":"Plant architecture changes to adapt to drought stress. Jasmonic acid (JA) is an endogenous growth regulator that alters plant development in response to changing environments. Teosinte BRANCHED1 (TB1)/BRANCHED 1 (BRC1) can integrate multiple signalling pathways. However, the molecular mechanism by which and how drought stress regulates the development of axillary buds and the relationship between JA and BRC1 remain elusive. In this study, we found that the development of axillary buds in chrysanthemum (Chrysanthemum morifolium) is inhibited under drought stress and after exogenous spraying of methyl jasmonate (MeJA). RNA-seq analysis revealed the response of CmBRC1b to this process. Molecular validation confirmed that CmMYC2 directly binds to the promoter of CmBRC1b and promotes its expression in vitro and in vivo, but CmMYC2 cannot bind to the CmBRC1a promoter. Genetic transformation confirmed that both CmMYC2 and CmBRC1b inhibit lateral bud outgrowth. We conclude that JA signalling inhibits the development of axillary buds through the CmMYC2-CmBRC1b module under drought stress. These findings reveal the molecular mechanisms by which plants respond to drought and alter axillary shoot development, which provide a strategy for developing cultivars with high resistance to drought stress and high yield.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"18 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The plant Cleavage Factor I complex is essential for precise cleavage and polyadenylation site determination 植物切割因子I复合体是精确切割和聚腺苷化位点测定所必需的

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-09 DOI: 10.1093/plphys/kiaf483

Lukasz Szewc, Xiaojuan Zhang, Mateusz Bajczyk, Dawid Bielewicz, Marta Zimna, Kei Yura, Mariko Kato, Mika Nomoto, Marta Garcia-León, Vicente Rubio, Yasuomi Tada, Tsuyoshi Furumoto, Takashi Aoyama, Zofia Szweykowska-Kulinska, Dorothee Staiger, Artur Jarmolowski, Tomohiko Tsuge

{"title":"The plant Cleavage Factor I complex is essential for precise cleavage and polyadenylation site determination","authors":"Lukasz Szewc, Xiaojuan Zhang, Mateusz Bajczyk, Dawid Bielewicz, Marta Zimna, Kei Yura, Mariko Kato, Mika Nomoto, Marta Garcia-León, Vicente Rubio, Yasuomi Tada, Tsuyoshi Furumoto, Takashi Aoyama, Zofia Szweykowska-Kulinska, Dorothee Staiger, Artur Jarmolowski, Tomohiko Tsuge","doi":"10.1093/plphys/kiaf483","DOIUrl":"https://doi.org/10.1093/plphys/kiaf483","url":null,"abstract":"Cleavage factor I (CFI) is a four-subunit protein complex of the pre-mRNA 3' end processing machinery in eukaryotes. In Arabidopsis (Arabidopsis thaliana), AtCFI25a, AtCFI25b, AtCFI59, and AtCFI68 have been identified as potential components of AtCFI, in silico. Here, we showed that AtCFI25a, AtCFI59, and AtCFI68 are each able to pull down each other as components of CFI, revealing the plant CFI complex composition. Furthermore, either AtCFI59 or AtCFI68 was essential for nuclear localization of the smallest subunit, AtCFI25a. Mutants with single loss-of-function for AtCFI59 or AtCFI68 showed no visible differences compared to wild-type plants, while the double mutant displayed pleiotropic morphological defects, identical to those previously reported for AtCFI25a loss-of-function plants. Moreover, these morphological defects correlated with alterations in the usage of 3' UTR cleavage and polyadenylation sites. atcfi25a, the atcfi25a atcfi25b double mutant, and the atcfi59 atcfi68 double mutant showed widespread changes in the selection of cleavage and polyadenylation sites. In addition to the loss of diversity for 3' UTR usages in these mutants, proximal cleavage and polyadenylation sites were favored in most cases, leading to shorter 3' UTRs. In particular, genes involved in light intensity, light harvesting, photosynthesis, and cold responses showed significant dependence on AtCFI function. Interestingly, transcripts coding for AtCFI subunits showed altered 3' end processing in these mutants, suggesting a self-regulation function of AtCFI.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"9 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145255049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annexin D1 promotes potyvirus infection through interaction with nuclear inclusion protein b and Ca2+-dependent phosphorylation. 膜联蛋白D1通过与核包涵蛋白b和Ca2+依赖性磷酸化相互作用促进多病毒感染。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-09 DOI: 10.1093/plphys/kiaf458

De-Jie Cheng,Xin-Yang Chen,Carlos Kwesi Tettey,Song-Yu Jiang,Jun Jiang,Cheng-Wu Zou,Hai-Tao Cui,Yan-Ping Tian,Xiang-Dong Li

{"title":"Annexin D1 promotes potyvirus infection through interaction with nuclear inclusion protein b and Ca2+-dependent phosphorylation.","authors":"De-Jie Cheng,Xin-Yang Chen,Carlos Kwesi Tettey,Song-Yu Jiang,Jun Jiang,Cheng-Wu Zou,Hai-Tao Cui,Yan-Ping Tian,Xiang-Dong Li","doi":"10.1093/plphys/kiaf458","DOIUrl":"https://doi.org/10.1093/plphys/kiaf458","url":null,"abstract":"Annexins are a family of calcium- and phospholipid-binding proteins with immunomodulatory roles. However, whether annexins regulate plant virus infection has not been studied in detail. Here, we report that annexin D1 of Nicotiana benthamiana (NbANXD1) forms dimers and interacts with nuclear inclusion protein b (NIb) of tobacco vein banding mosaic virus (TVBMV) by binding the C domain of NIb. NIb can recruit NbANXD1 to the perinuclear region, and the NIb-NbANXD1 interaction complex is found to co-localize with TVBMV 6K2. Further analysis showed that phosphokinase 29 of N. benthamiana (NbKIN29) interacts with and specifically phosphorylates NbANXD1 at amino acid residues T204, T276, and S286. Furthermore, Ca2+ may regulate TVBMV infection by modulating the phosphorylation of NbANXD1 by NbKIN29. Gene silencing, overexpression, knockout, and mutation experiments showed that annexin D1 positively regulates potyviral replication and systemic infection. In summary, our study supports that annexin D1 is recruited to promote potyvirus infection through interaction with NIb and Ca2+-dependent phosphorylation by phosphokinase 29. Our results provide important insights into the potyvirus infections.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"35 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transactivation of Dek601 by the Opaque2-OHP2-EREB58-EREB177 module regulates kernel development in maize. 通过Opaque2-OHP2-EREB58-EREB177模块激活Dek601调控玉米籽粒发育。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-09 DOI: 10.1093/plphys/kiaf502

Qianhan Wei,Kaijian Fan,Anqi Zhao,Qinghui Fu,Rong Miao,Yawen Zhang,Xupeng Dou,Yuhao Su,Zhenjing Ren,Yunling Peng,Jianhua Wang,Yunjun Liu

{"title":"Transactivation of Dek601 by the Opaque2-OHP2-EREB58-EREB177 module regulates kernel development in maize.","authors":"Qianhan Wei,Kaijian Fan,Anqi Zhao,Qinghui Fu,Rong Miao,Yawen Zhang,Xupeng Dou,Yuhao Su,Zhenjing Ren,Yunling Peng,Jianhua Wang,Yunjun Liu","doi":"10.1093/plphys/kiaf502","DOIUrl":"https://doi.org/10.1093/plphys/kiaf502","url":null,"abstract":"BAHD acyltransferases are a specialized class of plant enzymes involved in the acylation of secondary metabolites, yet their roles in seed development remain poorly understood. In this study, we identified the defective kernel 601 (dek601) mutant in the maize (Zea mays) W22 genetic background, which exhibits defective embryo and endosperm development, leading to smaller and shrunken kernels compared to wild type (WT). Using positional cloning, we localized the causal gene, Zm00001d039535 (also known as Disorganized wall 1, Dow1), to chromosome 3. A mutation in this gene causes a frameshift and disrupts protein function, which was confirmed through complementation tests. Dek601 encodes a BAHD family acyltransferase, and its expression is regulated by the transcription factors Opaque2 (O2), Ethylene-Responsive Element Binding Protein 58 (EREB58), and Ethylene-Responsive Element Binding Protein 177 (EREB177). These factors bind to the Dek601 promoter and modulate its expression. Furthermore, DEK601 interacts with the Acyl-activating enzyme 19 (ZmAAE19), suggesting their potential functional synergy in downstream enzymatic processes. Metabolomic analysis of dek601 kernels revealed significant shifts in metabolic profiles, including a downregulation of carbohydrate compounds and an upregulation of amino acids and metabolites from the phenylpropanoid and flavonoid pathways. This study demonstrates that the Opaque2-OHP2-EREB58-EREB177 regulatory module controls Dek601 expression, and DEK601 and ZmAAE19 likely collaborate to influence metabolite biosynthesis, thereby regulating kernel development in maize.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"67 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatin accessibility and transcriptome analyses reveal stress-responsive regulatory elements in Zygophyllum xanthoxylum. 染色质可及性和转录组分析揭示了木本叶黄素的应激响应调控元件。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-09 DOI: 10.1093/plphys/kiaf503

Tian-Qiong Wang,Xue-Ran Peng,Hengbin Gao,Wei Li,Suo-Min Wang,Yujun Wu,Ying Ju Li

{"title":"Chromatin accessibility and transcriptome analyses reveal stress-responsive regulatory elements in Zygophyllum xanthoxylum.","authors":"Tian-Qiong Wang,Xue-Ran Peng,Hengbin Gao,Wei Li,Suo-Min Wang,Yujun Wu,Ying Ju Li","doi":"10.1093/plphys/kiaf503","DOIUrl":"https://doi.org/10.1093/plphys/kiaf503","url":null,"abstract":"The adaptive evolution of xerophytes to abiotic stresses is intimately linked with evolutionarily conserved cis-regulatory elements (CREs). However, the key CREs and conserved non-coding sequences (CNSs) involved in stress responses remain largely unexplored. In this study, we investigated the epigenetic regulatory mechanisms of the xerophyte Zygophyllum xanthoxylum under abiotic stress using an integrated multi-omics approach. Using ATAC-seq, we mapped genome-wide chromatin accessibility profiles in shoots and roots under heat, salt, and drought treatments, identifying 2,423-77,497 high-quality accessible chromatin regions (ACRs). Zygophyllum xanthoxylum employs distinct chromatin remodeling strategies for different stresses: chromatin tends to open under salt and drought conditions but close under heat stress. By integrating RNA-seq data, we correlated ACR dynamics with gene expression changes and identified significant alterations in transcription factor binding profiles within these accessible regions. We validated a stress-responsive regulatory pathway where the ZxNF-YC10-ZxAPK1 module induces promoter-associated ACR opening, leading to gene expression upregulation. Comparative genomic analysis across five xerophyte species identified 165,896 phylogenetically conserved non-coding sequences containing critical transcription factor binding sites. These species-specific CNSs were significantly enriched within central regions of ACRs and exhibited higher overlap with root ACRs, suggesting they may represent crucial CREs that evolved during adaptation to adverse environments. Our study provides a comprehensive chromatin accessibility atlas for Z. xanthoxylum under diverse abiotic stresses and establishes cross-species CNS resources that may facilitate genetic improvement of stress-tolerant crops.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"114 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular MIZU-KUSSEI1 movement and hydrotropism in Arabidopsis require F-actin organization. 拟南芥细胞内MIZU-KUSSEI1的运动和嗜水需要F-actin组织。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-08 DOI: 10.1093/plphys/kiaf495

Kotaro Akita,Yutaka Miyazawa

{"title":"Intracellular MIZU-KUSSEI1 movement and hydrotropism in Arabidopsis require F-actin organization.","authors":"Kotaro Akita,Yutaka Miyazawa","doi":"10.1093/plphys/kiaf495","DOIUrl":"https://doi.org/10.1093/plphys/kiaf495","url":null,"abstract":"Tropism is directed growth toward or away from stimuli such as light, gravity, and moisture gradient, by which plants can adapt to their surroundings. Hydrotropism is a response to a moisture gradient across the soil, which enables plants to grow their roots towards wet soil and thus avoid drought conditions. MIZU-KUSSEI 1 (MIZ1) is a pivotal gene for root hydrotropism, and its function inside cortical cells at the transition zone is indispensable for hydrotropic bending. However, how MIZ1 is regulated in the cortical cells remains unclear. Here, we found that treatment with the actin depolymerizing drug latrunculin B (Lat B) reduces hydrotropic bending of the Arabidopsis (Arabidopsis thaliana) wild-type and a MIZ1 overexpression line. Moreover, lines with knocked down actin depolymerizing factors showed enhanced hydrotropic root bending, partly due to an increase in MIZ1 expression. We further explored intracellular MIZ1 dynamics using a GFP-fused MIZ1 (MIZ1-GFP) expressing line and found that MIZ1-GFP moves in the cytosol. Lat B treatment diminished MIZ1-GFP movement, indicating the movement of MIZ1-GFP is actin-dependent. These results indicate that actin filaments are required for proper hydrotropic root bending, probably by controlling MIZ1 expression and localization.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"4 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding microbiome shifts and their impacts on plant health during pathogen infections. 了解病原菌感染期间微生物组的变化及其对植物健康的影响。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-08 DOI: 10.1093/plphys/kiaf498

Daniela P T Thomazella,Letícia B Pereira,Paulo J P L Teixeira

{"title":"Understanding microbiome shifts and their impacts on plant health during pathogen infections.","authors":"Daniela P T Thomazella,Letícia B Pereira,Paulo J P L Teixeira","doi":"10.1093/plphys/kiaf498","DOIUrl":"https://doi.org/10.1093/plphys/kiaf498","url":null,"abstract":"Pathogen infections can drastically reshape plant-associated microbiomes, yet the mechanisms underlying these shifts and their consequences for plant health remain elusive. In this review, we integrate recent advances to delineate how pathogen attack drives both local and systemic microbiome restructuring via three major processes: (i) collateral effects of the plant immune system, including alterations to the local physicochemical niche; (ii) active recruitment of beneficial microbes through pathogen-induced root exudates (primary and secondary metabolites, volatiles and organic compounds); and (iii) pathogen-mediated manipulation of the host microbiome through microbial effectors, antimicrobial production, or niche competition. By contrasting adaptive outcomes (enrichment of disease-suppressive taxa) with detrimental consequences (dysbiosis, mutualist loss or proliferation of disease-promoting microbes), we emphasize the need for time-resolved, mechanistic studies that move beyond correlative surveys. Although the enrichment of protective microbes is a recurring theme in current literature, it is important to recognize that not all microbiome shifts are adaptive or host-directed. Considering alternative explanations is critical to avoid survivorship bias and to accurately interpret microbiome dynamics in the context of plant-pathogen interactions. Building on these insights, we discuss the rational design of microbiome-based interventions to enhance crop resilience, including synthetic microbial consortia and elicitor-mediated recruitment of beneficial microbes. Notably, integrating microbiome dynamics into plant pathology offers a promising path toward sustainable disease management, transforming the microbiome from a passive background into an active, designable component of plant immunity.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"4 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Paulinella chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold Paulinella染色质转运肽part2采用类似于γ-谷氨酰胺环转移酶折叠的结构折叠

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-08 DOI: 10.1093/plphys/kiaf504

Victoria Klimenko, Jens Reiners, Violetta Applegate, Katharina Reimann, Grzegorz Popowicz, Astrid Hoeppner, Athanasios Papadopoulos, Sander H J Smits, Eva C M Nowack

{"title":"The Paulinella chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold","authors":"Victoria Klimenko, Jens Reiners, Violetta Applegate, Katharina Reimann, Grzegorz Popowicz, Astrid Hoeppner, Athanasios Papadopoulos, Sander H J Smits, Eva C M Nowack","doi":"10.1093/plphys/kiaf504","DOIUrl":"https://doi.org/10.1093/plphys/kiaf504","url":null,"abstract":"The chromatophores of the cercozoan amoeba Paulinella are photosynthetic organelles that evolved from a cyanobacterial endosymbiont. Many nucleus-encoded chromatophore-targeted proteins carry unusual N-terminal targeting signals termed crTPs, which are bipartite. crTPpart1 likely mediates trafficking through the secretory pathway and is cleaved off during import, but crTPpart2 remains attached to its cargo protein and its function is unknown. To unravel the functional role of crTPpart2, here we elucidated the structures of crTPpart2 from two different chromatophore-targeted proteins by X-ray crystallography at ∼2.3 Å resolution. Interestingly, the crTPpart2 of both proteins adopts a structural fold. Both structures share a conserved structured core and a flexible N-terminal arm. The structured core resembles proteins of the γ-glutamyl cyclotransferase superfamily within which crTPpart2 structures form a protein (sub)-family. The proposed catalytic center typical for proteins with cyclotransferase activity is not conserved in crTPpart2. A Cys pair that is conserved in crTPpart2 of many chromatophore-targeted proteins has been captured as a disulfide bridge. Together, our data suggest that chromatophore-targeted proteins are imported in their folded state and that the fold adopted by crTPpart2 plays a functional role during import. The characterization of its structure and flexibility provides important steps towards elucidating this protein translocation mechanism.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"42 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145260580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0