Plant Physiology最新文献

Chloroplast stress caused by maltose hyperaccumulation activates chlorophagy via the core autophagy machinery. 麦芽糖过度积累引起的叶绿体应激通过核心自噬机制激活叶绿体自噬。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-05-08 DOI: 10.1093/plphys/kiag271

Sakuya Nakamura, Mayumi Wakazaki, Mayuko Sato, Kiminori Toyooka, Atsushi J Nagano, Hiroyuki Ishida, Shinya Hagihara, Masanori Izumi

{"title":"Chloroplast stress caused by maltose hyperaccumulation activates chlorophagy via the core autophagy machinery.","authors":"Sakuya Nakamura, Mayumi Wakazaki, Mayuko Sato, Kiminori Toyooka, Atsushi J Nagano, Hiroyuki Ishida, Shinya Hagihara, Masanori Izumi","doi":"10.1093/plphys/kiag271","DOIUrl":"https://doi.org/10.1093/plphys/kiag271","url":null,"abstract":"Chlorophagy is an autophagy pathway that delivers chloroplast components into the vacuole for degradation, thus eliminating damaged chloroplasts. Chloroplast degradation is observed in Arabidopsis (Arabidopsis thaliana) mutants of MALTOSE-EXCESS 1 (MEX1), a maltose exporter in the chloroplast inner envelope membrane. However, whether autophagy is involved in the mex1 phenotypes is unknown. To extend our understanding of the signals that emanate from damaged chloroplasts and activate chlorophagy, we investigated how mex1 chloroplasts are degraded. Chlorotic mature leaves caused by maltose hyperaccumulation in mex1 plants contained swollen chloroplasts in the cytoplasm and degrading chloroplasts in the vacuole, together with heightened expression of autophagy-related (ATG) genes. The vacuolar degradation of mex1 chloroplasts required the core ATG proteins ATG7 and ATG10. ATG8-labeled structures accumulated on the surfaces of swollen mex1 chloroplasts. These findings indicate that maltose hyperaccumulation triggers chlorophagy via the core autophagy machinery. Notably, phenotypic analysis of mex1 atg double mutant plants suggested that excess chlorophagy aggravates the chlorosis seen in mex1 leaves. Transcriptome deep sequencing indicated that maltose-excess stress shares a similar transcriptomic response with high-light stress, which also triggers chlorophagy. Therefore, the signals inducing chlorophagy may be highly stimulated in mex1 leaves, making mex1 mutants effective tools for chlorophagy research.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HATs off to OsHAG704 and OsBPM2: Novel regulators of OsRpp30 in Rice Blast Resistance. OsHAG704和OsBPM2：水稻抗稻瘟病的新调控因子。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-05-07 DOI: 10.1093/plphys/kiag268

Catherine P Freed

引用次数: 0

Photosystem I photoinhibition attenuates LHCI-dependent light-harvesting activity in spinach leaves. 光系统I光抑制减弱了菠菜叶片中lhci依赖的光收获活性。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-05-06 DOI: 10.1093/plphys/kiag251

Daisuke Takagi, Aya Kishie, Kentaro Ifuku

{"title":"Photosystem I photoinhibition attenuates LHCI-dependent light-harvesting activity in spinach leaves.","authors":"Daisuke Takagi, Aya Kishie, Kentaro Ifuku","doi":"10.1093/plphys/kiag251","DOIUrl":"https://doi.org/10.1093/plphys/kiag251","url":null,"abstract":"The over-reduced state of photosystem I causes photoinhibition, and changes in electron transport activity and core-subunit content have been studied to examine the degree of photosystem I photoinhibition. However, the involvement of peripheral subunits, such as light-harvesting complex I, in photosystem I photoinhibition has not been thoroughly evaluated. Here, we aimed to determine whether the light-harvesting functions of the light-harvesting complex I are altered by photosystem I photoinhibition in spinach leaves. To this end, we developed a method to estimate the functional antenna size of light-harvesting complex I by measuring the far-red light-dependent absorption change in the oxidized P700 reaction center chlorophyll in leaves in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea and methyl viologen. We analyzed kinetics using the double Gompertz model. Subsequently, we found that the rate constant and relative rate for P700+ induction (kmajor and Vmajor) increased linearly with an increase in the illuminated far-red light intensity. The Arabidopsis lhca4 mutant demonstrated that kmajor and Vmajor are suppressed compared to wild-type plants, suggesting that these parameters reflect light-harvesting activity depending on light-harvesting complex I. Following photoinhibitory treatment of photosystem I, spinach leaves showed reduced kmajor and Vmajor. The 77 K fluorescence emission spectra analysis showed blue-shifted fluorescence at approximately 740 nm after photosystem I photoinhibition, suggesting that the light energy transfer process from light-harvesting complex I to the PSI-core is disturbed because of photosystem I photoinhibition. From these observations, we propose that the light-harvesting complex I, as well as its core subunits, are targets of photosystem I photoinhibition.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A precise splice: Proper splicing patterns required for appropriate lipid metabolism during callus induction with cytokinin. 精确剪接：在细胞分裂素诱导愈伤组织过程中，适当的脂质代谢需要适当的剪接模式。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-05-06 DOI: 10.1093/plphys/kiag270

Alyssa Kearly

引用次数: 0

Salicylic acid moonlights beyond defense to guard rice seed longevity. 水杨酸在保护水稻种子寿命方面发挥着超越防御的作用。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-05-06 DOI: 10.1093/plphys/kiag267

Neeta Lohani

引用次数: 0

Overexpression of β-carboxysomes increases photosynthesis and growth in Synechocystis sp. PCC 6803. β-羧酸体的过表达增加了Synechocystis sp. PCC 6803的光合作用和生长。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-05-06 DOI: 10.1093/plphys/kiag261

Jinlu Hu, Kuo Zhao, Yu Chen, Xingwu Ge, Jing Yang, Gregory F Dykes, Jian-Yu Shi, Lu-Ning Liu

{"title":"Overexpression of β-carboxysomes increases photosynthesis and growth in Synechocystis sp. PCC 6803.","authors":"Jinlu Hu, Kuo Zhao, Yu Chen, Xingwu Ge, Jing Yang, Gregory F Dykes, Jian-Yu Shi, Lu-Ning Liu","doi":"10.1093/plphys/kiag261","DOIUrl":"https://doi.org/10.1093/plphys/kiag261","url":null,"abstract":"Cyanobacteria are photoautotrophic cell factories capable of converting carbon dioxide into valuable chemicals. The rate-limiting step in photosynthesis is the enzymatic dark reaction catalyzed by ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO). The carboxysome is a protein-based organelle that plays a central role in cyanobacterial carbon fixation. It consists of thousands of subunits, including hexameric and pentameric proteins, that form a shell to encapsulate the enzymes RuBisCO and carbonic anhydrase. Here, we enhanced β-carboxysome biogenesis in Synechocystis sp. PCC 6803 by overexpressing a full or partial set of endogenous β-carboxysome components. Overexpression of carboxysome proteins altered protein stoichiometry, morphology, and cellular abundance of β-carboxysomes, as well as improved photosynthetic activities and cell growth. These findings indicate that targeted carboxysome overexpression enhances photosynthetic efficiency and growth of cyanobacteria under favorable conditions. This study provides a framework for engineering cyanobacterial chassis cells as microbial cell factories and carboxysome-based CO2-concentrating mechanisms in heterologous hosts, including crop plants, to improve photosynthetic productivity for sustainable bioproduction.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin glycosylation and hydrolysis mediate a biochemical arms race between apple and herbivorous insects. 槲皮素糖基化和水解介导了苹果和草食性昆虫之间的生化军备竞赛。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-05-06 DOI: 10.1093/plphys/kiag273

Jing Xu, Shanshan Zhao, Xiaoping Zhu, Fengwang Ma, Jia-Long Yao, Pengmin Li

{"title":"Quercetin glycosylation and hydrolysis mediate a biochemical arms race between apple and herbivorous insects.","authors":"Jing Xu, Shanshan Zhao, Xiaoping Zhu, Fengwang Ma, Jia-Long Yao, Pengmin Li","doi":"10.1093/plphys/kiag273","DOIUrl":"https://doi.org/10.1093/plphys/kiag273","url":null,"abstract":"Quercetin glycosides are abundant flavonoids in apple (Malus domestica) leaves; however, how their structural variation shapes plant-insect interactions remains unclear. Here, we show that quercetin glycosylation and hydrolysis underlie a biochemical arms race between apple and multiple herbivorous insects. Herbivory by Helicoverpa armigera and Spodoptera litura activated a jasmonic acid (JA)-regulated transcriptional response that selectively induced the quercetin-glycoside pathway, resulting in elevated accumulation of five major quercetin glycosides. Methyl jasmonate treatment and JA content-boosting transgenic plants increased quercetin glycosides, whereas altering quercetin glycoside content did not feed back to JA signaling. Functional analyses revealed strong structure-dependent variation in anti-insect activity. Quercetin 3-O-glucoside (Q3Glc), but not its isomer quercetin 3-O-galactoside (Q3Gal), deterred larval feeding. During herbivory, apple leaves hydrolyzed Q3Glc to release toxic quercetin, while insects displayed species-specific detoxification strategies. H. armigera and S. exigua re-glycosylated quercetin to regenerate Q3Glc, neutralizing toxicity, whereas S. litura and S. frugiperda lacked this ability and suffered reduced growth. These findings reveal a reciprocal metabolic interplay in which apple activates glycoside hydrolysis to deploy toxic aglycones, while insects counter-evolve re-glycosylation to attenuate toxicity. Quercetin glycosylation patterns, therefore, serve as dynamic determinants of apple defense and herbivore susceptibility, offering opportunities for breeding insect-resistant cultivars.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-mRNA splicing regulates cellular dedifferentiation via lipid metabolism in a cytokinin-dependent manner in Arabidopsis. Pre-mRNA剪接以细胞分裂素依赖的方式通过脂质代谢调节细胞去分化。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-05-06 DOI: 10.1093/plphys/kiag269

Ami Takeuchi, Toshiki Ishikawa, Toshihiro Arae, Junichi Togami, Kenji Nagamiya, Koji Koizumi, Takuyuki Ikeda, Shingo Nagawa, Iwai Ohbayashi, Munetaka Sugiyama, Misato Ohtani

{"title":"Pre-mRNA splicing regulates cellular dedifferentiation via lipid metabolism in a cytokinin-dependent manner in Arabidopsis.","authors":"Ami Takeuchi, Toshiki Ishikawa, Toshihiro Arae, Junichi Togami, Kenji Nagamiya, Koji Koizumi, Takuyuki Ikeda, Shingo Nagawa, Iwai Ohbayashi, Munetaka Sugiyama, Misato Ohtani","doi":"10.1093/plphys/kiag269","DOIUrl":"https://doi.org/10.1093/plphys/kiag269","url":null,"abstract":"Plant cells exhibit high plasticity for proliferation and differentiation, and pre-mRNA splicing makes an important contribution to this plasticity. Here, we show that the gene responsible for root redifferentiation defective4-1 (rrd4-1), a temperature-sensitive Arabidopsis thaliana mutant with defects in adventitious rooting and callus formation from hypocotyls, encodes a homolog of yeast Ntr1, which is involved in pre-mRNA splicing. Defective callus formation in rrd4-1 at the restrictive temperature of 28°C depended on the presence of kinetin (a synthetic cytokinin) in the callus-inducing medium. RNA-seq analysis revealed that genes involved in secondary cell wall biogenesis were upregulated, whereas those involved in cell cycle progression were downregulated, in a cytokinin-dependent manner in rrd4-1 at 28°C. Moreover, kinetin and the rrd4-1 mutation had broad effects on alternative splicing, particularly on lipid metabolism genes such as PTPLA, SPHK2, and ATNCER1. Consistent with this result, levels of very-long-chain fatty acid (VLCFA)-type sphingolipids were reduced in rrd4-1, and kinetin affected their contents during callus induction. Kinetin enhanced the inhibitory effect of the lipid synthesis inhibitor cerulenin on callus formation, and rrd4-1 was hypersensitive to cerulenin. Together, our data suggest that pre-mRNA splicing regulates cytokinin-mediated cellular dedifferentiation through the regulation of lipid metabolism gene splicing.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

StWRKY46 promotes stomatal closure and enhances potato drought tolerance by mediating the PYL1-ABI2-OST1/SnRK2.5/SnRK2.6 pathway. StWRKY46通过介导PYL1-ABI2-OST1/SnRK2.5/SnRK2.6通路促进气孔关闭，增强马铃薯抗旱性。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-05-06 DOI: 10.1093/plphys/kiag265

Han Wei, Xiao Wang, Shigui Li, Ning Zhang, Huaijun Si

{"title":"StWRKY46 promotes stomatal closure and enhances potato drought tolerance by mediating the PYL1-ABI2-OST1/SnRK2.5/SnRK2.6 pathway.","authors":"Han Wei, Xiao Wang, Shigui Li, Ning Zhang, Huaijun Si","doi":"10.1093/plphys/kiag265","DOIUrl":"https://doi.org/10.1093/plphys/kiag265","url":null,"abstract":"Drought stress affects crop yield and quality. Abscisic acid (ABA) plays a crucial role in plant responses to drought stress; however, the signal transduction mechanism in potato (Solanum tuberosum L.) remains unclear. This study identifies a WRKY transcription factor (TF) member, StWRKY46, that enhances drought tolerance in potato. Under drought, StWRKY46 binds directly to the promoter of StPYL1 (a PYR/PYL family ABA receptor) and activates its transcription, triggering the ABA signaling pathway and improving drought tolerance in potato. StPYL1 interacts with StHAB1, StPP2C24, StPP2C51.1, and StABI2. StABI2 acts as a key negative regulator of ABA signaling. It interacts with StOST1, StSnRK2.5, and StSnRK2.6 to regulate the expression of stomatal movement-related genes, including the slow-type anion channel 1 (StSLAC1) and guard cell hydrogen peroxide-resistant 1 (StGHR1), thereby potentiating ABA-induced stomatal closure. Overall, our findings demonstrate the molecular mechanism of StWRKY46-mediated drought tolerance through the ABA-StPYL1-StABI2-StOST1/StSnRK2.5/StSnRK2.6 pathway in potato.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity in Rubisco Kinetics and CO2-Concentrating Mechanisms in Cyanobacteria. 蓝藻中Rubisco动力学和co2富集机制的多样性。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-04-30 DOI: 10.1093/plphys/kiag259

Pere Aguiló-Nicolau, Reto S Wijker, Concepción Iñiguez, Sebastià Capó-Bauçà, Heather M Stoll, Jeroni Galmés

{"title":"Diversity in Rubisco Kinetics and CO2-Concentrating Mechanisms in Cyanobacteria.","authors":"Pere Aguiló-Nicolau, Reto S Wijker, Concepción Iñiguez, Sebastià Capó-Bauçà, Heather M Stoll, Jeroni Galmés","doi":"10.1093/plphys/kiag259","DOIUrl":"https://doi.org/10.1093/plphys/kiag259","url":null,"abstract":"Cyanobacteria are the most ancient oxygenic photosynthetic organisms on Earth and play a pivotal role in the global carbon cycle. Despite their ecological and evolutionary significance, the mechanisms of carbon acquisition and fixation in this phylum remain largely unexplored beyond a few model species. Here, we examined representative taxa spanning the full phylogenetic breadth of Cyanobacteria, assessing in vivo carbon-acquisition pathways, the role and effectiveness of CO2-concentrating mechanisms (CCMs), as well as conducting in vitro biochemical characterizations of the kinetic traits and carbon isotope fractionation of Rubisco. We found significant strain-specific differences in Rubisco kinetics and CCM performance, but a common signature of high Rubisco catalytic turnover coupled with low CO2 affinity, consistent with the co-evolution of this enzyme together with effective CCMs. Furthermore, we identified a strong positive correlation between Rubisco intrinsic carbon isotope fractionation and its CO2/O2 specificity factor. Together, these results provide insight into Rubisco catalysis and shed light on its co-evolution with CCMs, underscoring their role in shaping Earth's carbon dynamics.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0