Plant Physiology最新文献

CELL DIVISION CYCLE 5 controls floral transition by regulating flowering gene transcription and splicing in Arabidopsis. 细胞分裂周期 5 通过调控拟南芥开花基因的转录和剪接来控制花期转换。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-19 DOI: 10.1093/plphys/kiae616

Xin Xin, Linhan Ye, Tingting Zhai, Shu Wang, Yunjiao Pan, Ke Qu, Mengjie Gu, Yanjiao Wang, Jiedao Zhang, Xiang Li, Wei Yang, Shuxin Zhang

{"title":"CELL DIVISION CYCLE 5 controls floral transition by regulating flowering gene transcription and splicing in Arabidopsis.","authors":"Xin Xin, Linhan Ye, Tingting Zhai, Shu Wang, Yunjiao Pan, Ke Qu, Mengjie Gu, Yanjiao Wang, Jiedao Zhang, Xiang Li, Wei Yang, Shuxin Zhang","doi":"10.1093/plphys/kiae616","DOIUrl":"10.1093/plphys/kiae616","url":null,"abstract":"CELL DIVISION CYCLE 5 (CDC5) is a R2R3-type MYB transcription factor, serving as a key component of Modifier of snc1, 4 (MOS4)-associated complex (MAC)/NineTeen Complex (NTC), which is associated with plant immunity, RNA splicing, and miRNA biogenesis. In this study, we demonstrate that mutation of CDC5 accelerates flowering in Arabidopsis (Arabidopsis thaliana). CDC5 activates the expression of FLOWERING LOCUS C (FLC) by binding to and affecting the enrichment of RNA polymerase II on FLC chromatin. Moreover, genetic analysis confirmed that CDC5 regulates flowering in an FLC-dependent manner. Furthermore, we characterized the interaction of CDC5 with the RNA polymerase-associated factor 1 (Paf1) complex and confirmed that CDC5, as part of the spliceosome, mediates genome-wide alternative splicing, as revealed by RNA-Seq. CDC5 affected the splicing of flowering-associated genes such as FLC, SEF, and MAFs. Additionally, we also demonstrated that CDC5 contributes to the regulation of histone modification of FLC chromatin, which further promotes FLC expression. In summary, our results establish CDC5 as a key factor regulating flowering. This provides valuable insight for future research into plant flowering.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclic and pseudo-cyclic electron pathways play antagonistic roles during nitrogen deficiency in Chlamydomonas reinhardtii. 环状电子途径和假环状电子途径在莱茵衣藻缺氮过程中发挥拮抗作用

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-19 DOI: 10.1093/plphys/kiae617

Ousmane Dao, Adrien Burlacot, Felix Buchert, Marie Bertrand, Pascaline Auroy, Carolyne Stoffel, Sai Kiran Madireddi, Jacob Irby, Michael Hippler, Gilles Peltier, Yonghua Li-Beisson

{"title":"Cyclic and pseudo-cyclic electron pathways play antagonistic roles during nitrogen deficiency in Chlamydomonas reinhardtii.","authors":"Ousmane Dao, Adrien Burlacot, Felix Buchert, Marie Bertrand, Pascaline Auroy, Carolyne Stoffel, Sai Kiran Madireddi, Jacob Irby, Michael Hippler, Gilles Peltier, Yonghua Li-Beisson","doi":"10.1093/plphys/kiae617","DOIUrl":"10.1093/plphys/kiae617","url":null,"abstract":"Nitrogen (N) scarcity frequently constrains global biomass productivity. N deficiency halts cell division, downregulates photosynthetic electron transfer, and enhances carbon storage. However, the molecular mechanism downregulating photosynthesis during N deficiency and its relationship with carbon storage are not fully understood. Proton Gradient Regulator-like 1 (PGRL1) controlling cyclic electron flow (CEF) and Flavodiiron proteins (FLV) involved in pseudo-CEF (PCEF) are major players in the acclimation of photosynthesis. To determine the role of PGRL1 or FLV in photosynthesis under N deficiency, we measured photosynthetic electron transfer, oxygen gas exchange, and carbon storage in Chlamydomonas reinhardtii pgrl1 and flvB knockout mutants. Under N deficiency, pgrl1 maintained higher net photosynthesis and O2 photoreduction rates and higher levels of Cytochrome b6f and PSI compared to the control and flvB. The photosynthetic activity of flvB and pgrl1 flvB double mutants decreased in response to N deficiency, similar to the control strains. Furthermore, the preservation of photosynthetic activity in pgrl1 was accompanied by an increased accumulation of triacylglycerol in certain genetic backgrounds but not others, highlighting the importance of gene-environment interaction in determining traits such as oil content. Our results suggest that in the absence of PGRL1-controlled CEF, FLV-mediated PCEF maintains net photosynthesis at a high level and that CEF and PCEF play antagonistic roles during N deficiency. They further illustrate how a strain's nutrient status and genetic makeup can affect regulation of photosynthetic energy conversion in relation to carbon storage and provide additional strategies for improving lipid productivity in algae.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The E3 ubiquitin ligase COP1 and transcription factors HY5 and RHD6 integrate light signaling and root hair development. E3 泛素连接酶 COP1 以及转录因子 HY5 和 RHD6 整合了光信号和根毛的发育。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-19 DOI: 10.1093/plphys/kiae618

Tianen Zhang, Jingjuan Zhu, Yang Liu, Yanfei Pei, Yayue Pei, Zhenzhen Wei, Pengfei Miao, Jun Peng, Fuguang Li, Zhi Wang

{"title":"The E3 ubiquitin ligase COP1 and transcription factors HY5 and RHD6 integrate light signaling and root hair development.","authors":"Tianen Zhang, Jingjuan Zhu, Yang Liu, Yanfei Pei, Yayue Pei, Zhenzhen Wei, Pengfei Miao, Jun Peng, Fuguang Li, Zhi Wang","doi":"10.1093/plphys/kiae618","DOIUrl":"10.1093/plphys/kiae618","url":null,"abstract":"Light signaling plays a substantial role in regulating plant development, including the differentiation and elongation of single-celled tissue. However, the identity of the regulatory machine that affects light signaling on root hair cell (RHC) development remains unclear. Here, we investigated how darkness inhibits differentiation and elongation of RHC in Arabidopsis (Arabidopsis thaliana). We found that light promotes the growth and development of RHC. RNA-seq analysis showed that light signaling regulates the differentiation of RHC by promoting the expression of specific genes in the root epidermis associated with cell wall remodeling, JA, auxin, and ethylene signaling pathways. Together, these genes integrate light and phytohormone signals with root hair development. Our investigation also revealed that the core light signal factor ELONGATED HYPOCOTYL 5 (HY5) directly interacts with the key root hair development factor ROOT HAIR DEFECTIVE6 (RHD6), which promotes the transcription of RSL4. However, CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) repressed RHD6 function through the COP1-HY5 complex. Our genetic studies confirm associations between RHD6, HY5, and COP1, indicating that RHD6 largely depends on HY5 for root hair development. Ultimately, our work suggests a central COP1-HY5-RHD6 regulatory module that integrates light signaling and root hair development with several downstream pathways, offering perspectives to decipher single-celled root hair development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The wheat CC-NBS-LRR protein TaRGA3 confers resistance to stripe rust by suppressing Ascorbate peroxidase 6 activity. 小麦 CC-NBS-LRR 蛋白 TaRGA3 通过抑制抗坏血酸过氧化物酶 6 的活性来增强对条锈病的抗性。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-18 DOI: 10.1093/plphys/kiae603

Nannan Fang, Conghui Jia, Ruolin Chen, Jiarui An, Zhensheng Kang, Jie Liu

{"title":"The wheat CC-NBS-LRR protein TaRGA3 confers resistance to stripe rust by suppressing Ascorbate peroxidase 6 activity.","authors":"Nannan Fang, Conghui Jia, Ruolin Chen, Jiarui An, Zhensheng Kang, Jie Liu","doi":"10.1093/plphys/kiae603","DOIUrl":"10.1093/plphys/kiae603","url":null,"abstract":"Nucleotide-binding leucine-rich repeat (NLR) proteins are intracellular immune receptors that activate innate immune responses upon sensing pathogen attack. However, the molecular mechanisms by which NLR proteins initiate downstream signal transduction pathways to counteract pathogen invasion remain poorly understood. In this study, we identified the wheat (Triticum aestivum) NLR protein Resistance Gene Analogs3 (TaRGA3), which was significantly up-regulated during Puccinia striiformis f. sp. tritici (Pst) infection. TaRGA3 and its coiled-coil (CC) domain, localized to the cytoplasm and nucleus, can induce cell death in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) and overexpression suggested that TaRGA3 contributed to wheat resistance to stripe rust by facilitating reactive oxygen species (ROS) accumulation. Yeast two-hybrid, luciferase complementation imaging, and co-immunoprecipitation assays revealed that TaRGA3 interacted with wheat protein Ascorbate Peroxidase 6 (TaAPX6). Further analysis showed that TaAPX6 specifically targeted the CC domain of TaRGA3. The TaRGA3-TaAPX6 interplay led to reduced enzyme activity of TaAPX6. Notably, TaAPX6 negatively regulated wheat resistance to Pst by removing excessive ROS accompanying Pst-induced hypersensitive responses. Our findings reveal that TaRGA3 responding to Pst infection confers enhanced wheat resistance to stripe rust, possibly by suppressing TaAPX6-modulated ROS scavenging, and demonstrate that TaRGA3 can be used to engineer stripe rust resistance in wheat.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The E3 ligase TaE3V-B1 ubiquitinates proteins encoded by the vernalization gene TaVRN1 and regulates developmental processes in wheat. E3连接酶TaE3V-B1泛素化春化基因TaVRN1编码的蛋白，并调控小麦的发育过程。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-18 DOI: 10.1093/plphys/kiae606

Tian Li, Ragupathi Nagarajan, Shujuan Liu, Juan C Luzuriaga, Wenxuan Zhai, Shuanghe Cao, Haiyan Jia, Brett F Carver, Liuling Yan

{"title":"The E3 ligase TaE3V-B1 ubiquitinates proteins encoded by the vernalization gene TaVRN1 and regulates developmental processes in wheat.","authors":"Tian Li, Ragupathi Nagarajan, Shujuan Liu, Juan C Luzuriaga, Wenxuan Zhai, Shuanghe Cao, Haiyan Jia, Brett F Carver, Liuling Yan","doi":"10.1093/plphys/kiae606","DOIUrl":"10.1093/plphys/kiae606","url":null,"abstract":"In wheat (Triticum aestivum), early maturity is desired to avoid the hot and dry summer season, especially in view of climate change. Here, we report that TaE3V1, a C3H2C3 RING-type E3 ligase that interacts with TaVRN1, is associated with early development. Aside from its RING domain, TaE3V1 does not harbor any domains that are conserved in other RING-type or other E3 ligase proteins. TaE3V-B1b, encoded by the functional TaE3V1 allele, interacts with and ubiquitinates TaVRN1. In contrast, TaE3V-B1a, encoded by a natural nonfunctional TaE3V1 allele, neither interacts with TaVRN1 nor has E3 ligase activity. TaE3V-B1b activity decreases with plant age under warmer temperatures, but not under the low temperatures required for vernalization. We employed a gene editing method to simultaneously inactivate the three homoeologous TaE3V1 genes to validate their functions. Overall, our results suggest that the naturally mutated and edited TaE3V1 alleles can accelerate wheat development and aid adaptation to warming climates.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EARLY FLOWERING 3 alleles affect the temperature responsiveness of the circadian clock in Chinese cabbage 早花 3 等位基因影响大白菜昼夜节律钟的温度响应性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-15 DOI: 10.1093/plphys/kiae505

Shan Wang, Daling Feng, Yakun Zheng, Yin Lu, Kailin Shi, Rui Yang, Wei Ma, Na Li, Mengyang Liu, Yanhua Wang, Yiguo Hong, C Robertson McClung, Jianjun Zhao

{"title":"EARLY FLOWERING 3 alleles affect the temperature responsiveness of the circadian clock in Chinese cabbage","authors":"Shan Wang, Daling Feng, Yakun Zheng, Yin Lu, Kailin Shi, Rui Yang, Wei Ma, Na Li, Mengyang Liu, Yanhua Wang, Yiguo Hong, C Robertson McClung, Jianjun Zhao","doi":"10.1093/plphys/kiae505","DOIUrl":"https://doi.org/10.1093/plphys/kiae505","url":null,"abstract":"Temperature is an environmental cue that entrains the circadian clock, adapting it to local thermal and photoperiodic conditions that characterize different geographic regions. Circadian clock thermal adaptation in leafy vegetables such as Chinese cabbage (Brassica rapa ssp. pekinensis) is poorly understood but essential to sustain and increase vegetable production under changing climates. We investigated circadian rhythmicity in natural Chinese cabbage accessions grown at 14, 20, and 28 °C. The circadian period was significantly shorter at 20 °C than at either 14 or 28 °C, and the responses to increasing temperature and temperature compensation (Q10) were associated with population structure. Genome-wide association studies mapping identified variation responsible for temperature compensation as measured by Q10 value for temperature increase from 20 to 28 °C. Haplotype analysis indicated that B. rapa EARLY FLOWERING 3 H1 Allele (BrELF3H1) conferred a significantly higher Q10 value at 20 to 28 °C than BrELF3H2. Co-segregation analyses of an F2 population derived from a BrELF3H1 × BrELF3H2 cross revealed that variation among BrELF3 alleles determined variation in the circadian period of Chinese cabbage at 20 °C. However, their differential impact on circadian oscillation was attenuated at 28 °C. Transgenic complementation in Arabidopsis thaliana elf3-8 mutants validated the involvement of BrELF3 in the circadian clock response to thermal cues, with BrELF3H1 conferring a higher Q10 value than BrELF3 H2 at 20 to 28 °C. Thus, BrELF3 is critical to the circadian clock response to ambient temperature in Chinese cabbage. These findings have clear implications for breeding new varieties with enhanced resilience to extreme temperatures.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"75 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factor PagWRKY33 regulates gibberellin signaling and immune receptor pathways in Populus. 转录因子 PagWRKY33 调节杨树赤霉素信号和免疫受体通路。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-06 DOI: 10.1093/plphys/kiae593

Xiao-Qian Yu, Hao-Qiang Niu, Yue-Mei Zhang, Xiao-Xu Shan, Chao Liu, Hou-Ling Wang, Weilun Yin, Xinli Xia

{"title":"Transcription factor PagWRKY33 regulates gibberellin signaling and immune receptor pathways in Populus.","authors":"Xiao-Qian Yu, Hao-Qiang Niu, Yue-Mei Zhang, Xiao-Xu Shan, Chao Liu, Hou-Ling Wang, Weilun Yin, Xinli Xia","doi":"10.1093/plphys/kiae593","DOIUrl":"10.1093/plphys/kiae593","url":null,"abstract":"Enhanced autoimmunity often leads to impaired plant growth and development, and the coordination of immunity and growth in Populus remains elusive. In this study, we have identified the transcription factors PagWRKY33a/b as key regulators of immune response and growth maintenance in Populus. The disruption of PagWRKY33a/b causes growth issues and autoimmunity while conferring resistance to anthracnose caused by Colletotrichum gloeosporioides. PagWRKY33a/b binds to the promoters of N requirement gene 1.1 (NRG1.1) and Gibberellic Acid-Stimulated in Arabidopsis (GASA14)during infection, activating their transcription. This process maintains disease resistance and engages in GA signaling to reduce growth costs from immune activation. The oxPagWRKY33a/nrg1.1 mutant results in reduced resistance to C. gloeosporioides. Further, PagWRKY33a/b is phosphorylated and activated by Mitogen-Activated Protein Kinase Kinase 1 (MKK1), which inhibits Respiratory Burst Oxidase Homolog D (RBOHD) and Respiratory Burst Oxidase Homolog I (RBOHI) transcription, causing ROS bursts in wrky33a/b double mutants. This leads to an upregulation of PagNRG1.1 in the absence of pathogens. However, the wrky33a/b/nrg1.1 and wrky33a/b/rbohd triple mutants show compromised defense responses, underscoring the complexity of WRKY33 regulation. Additionally, the stability of PagWRKY33 is modulated by Ring Finger Protein 5 (PagRNF5)-mediated ubiquitination, balancing plant immunity and growth. Together, our results provide key insights into the complex function of WRKY33 in Populus autoimmunity and its impact on growth and development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MYB168 and WRKY20 transcription factors synergistically regulate lignin monomer synthesis during potato tuber wound healing. MYB168 和 WRKY20 转录因子协同调控马铃薯块茎伤口愈合过程中木质素单体的合成。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-05 DOI: 10.1093/plphys/kiae573

Ruirui Yang, Qihui Wang, Ying Wang, Xuejiao Zhang, Xiaoyuan Zheng, Yongcai Li, Dov Prusky, Yang Bi, Ye Han

{"title":"MYB168 and WRKY20 transcription factors synergistically regulate lignin monomer synthesis during potato tuber wound healing.","authors":"Ruirui Yang, Qihui Wang, Ying Wang, Xuejiao Zhang, Xiaoyuan Zheng, Yongcai Li, Dov Prusky, Yang Bi, Ye Han","doi":"10.1093/plphys/kiae573","DOIUrl":"https://doi.org/10.1093/plphys/kiae573","url":null,"abstract":"Lignin is a critical component of the closing layer of the potato (Solanum tuberosum L.) tuber during healing; however, the molecular mechanism of its formation remains poorly understood. To elucidate the molecular mechanism of tuber healing, we screened the genes encoding transcription factors that regulate lignin synthesis(StMYB24/49/105/144/168, StWRKY19/20/22/23/34) and the key genes involved in lignin monomer synthesis (PHENYLALANINE AMMONIA LYASE 5 (StPAL5) and CINNAMYL ALCOHOL DEHYDROGENASE 14 (StCAD14)) for induced expression after wounding using transcriptome data. DLR, Y1H, EMSA, and ChIP-qPCR assays revealed that StMYB168 could bind directly to the StPAL5 and StCAD14 promoters to activate their expression and that StWRKY20 enhanced this regulation with a synergistic effect. Y2H, BiFC, and Co-IP assays showed that StMYB168 interacted with StWRKY20 to form a MYB-WRKY complex. Furthermore, transient overexpression of StMYB168 and StWRKY20 in Nicotiana benthamiana leaves upregulated the expression of NbPAL and NbCAD10 and promoted lignin accumulation in the leaves. In addition, overexpression of StWRKY20 and StMYB168 together resulted in higher expression levels of NbPAL and NbCAD10 and higher levels of lignin monomer and total lignin. In contrast, silencing of StMYB168 and StWRKY20 in potato significantly reduced the lignin content of wounded tubers. In conclusion, StMYB168 and StWRKY20 are important regulators of lignin biosynthesis in potato tubers during healing and can positively regulate lignin biosynthesis by forming a complex. The elucidation of this regulatory module provides information on the regulatory mechanism of lignin monomer synthesis in wounded tubers at the transcriptional level.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Flux Analysis to Increase Oil in Seeds. 通过代谢通量分析提高种子含油量

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-05 DOI: 10.1093/plphys/kiae595

Thiya Mukherjee, Shrikaar Kambhampati, Stewart A Morley, Timothy P Durrett, Doug K Allen

{"title":"Metabolic Flux Analysis to Increase Oil in Seeds.","authors":"Thiya Mukherjee, Shrikaar Kambhampati, Stewart A Morley, Timothy P Durrett, Doug K Allen","doi":"10.1093/plphys/kiae595","DOIUrl":"https://doi.org/10.1093/plphys/kiae595","url":null,"abstract":"Ensuring an adequate food supply and enough energy to sustainably support future global populations will require enhanced productivity from plants. Oilseeds can help address these needs; but the fatty acid composition of seed oils is not always optimal, and higher yields are required to meet growing demands. Quantitative approaches including metabolic flux analysis can provide insights on unexpected metabolism (i.e., when metabolism is different than in a textbook) and can be used to guide engineering efforts; however, as metabolism is context-specific, it changes with tissue type, local environment, and development. This review describes recent insights from metabolic flux analysis in oilseeds and indicates engineering opportunities based on emerging topics and developing technologies that will aid quantitative understanding of metabolism and enable efforts to produce more oil. We also suggest that investigating the key regulators of fatty acid biosynthesis, such as transcription factors, and exploring metabolic signals like phytohormones in greater depth through flux analysis, could open new pathways for advancing genetic engineering and breeding strategies to enhance oil crop production.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osmotic stress in roots drives lipoxygenase-dependent plastid remodeling through singlet oxygen production. 根中的渗透胁迫通过产生单线态氧驱动脂氧合酶依赖性质体重塑

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-05 DOI: 10.1093/plphys/kiae589

Dekel Cohen-Hoch, Tomer Chen, Lior Sharabi, Nili Dezorella, Maxim Itkin, Gil Feiguelman, Sergey Malitsky, Robert Fluhr

{"title":"Osmotic stress in roots drives lipoxygenase-dependent plastid remodeling through singlet oxygen production.","authors":"Dekel Cohen-Hoch, Tomer Chen, Lior Sharabi, Nili Dezorella, Maxim Itkin, Gil Feiguelman, Sergey Malitsky, Robert Fluhr","doi":"10.1093/plphys/kiae589","DOIUrl":"https://doi.org/10.1093/plphys/kiae589","url":null,"abstract":"Osmotic stress, caused by the lack of water or by high salinity, is a common problem in plant roots. Osmotic stress can be reproducibly simulated with the application of solutions of the high-molecular-weight and impermeable polyethylene glycol. The accumulation of different reactive oxygen species, such as singlet oxygen, superoxide, and hydrogen peroxide, accompany this stress. Among them, singlet oxygen, produced as a byproduct of lipoxygenase activity, has been associated with limiting root growth. To better understand the source and effect of singlet oxygen, we followed its production at the cellular level in Arabidopsis (Arabidopsis thaliana). Osmotic stress initiated profound changes in plastid and vacuole structure. Confocal and electron microscopy showed that the plastids were a source of singlet oxygen accompanied by the appearance of multiple, small extraplastidic bodies that were also an intense source of singlet oxygen. A marker protein, CRUMPLED LEAF, indicated that these small bodies originated from the plastid outer membrane. Remarkably, LINOLEATE 9S-LIPOXYGENASE 5, (LOX5), was shown to change its distribution from uniformly cytoplasmic to a more clumped distribution together with plastids and the small bodies. In addition, oxylipin products of type 9 lipoxygenase increased, while products of type 13 lipoxygenases decreased. Inhibition of lipoxygenase by the SHAM inhibitor or in down-regulated lipoxygenase lines prevented cells from initiating the cellular responses, leading to cell death. In contrast, singlet oxygen scavenging halted terminal cell death. These findings underscore the reversible nature of osmotic stress-induced changes, emphasizing the pivotal roles of lipoxygenases and singlet oxygen in root stress physiology.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0