Plant Physiology最新文献

Modeling Arabidopsis root growth and development

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-03-04 DOI: 10.1093/plphys/kiaf045

Marta Ibañes

引用次数: 0

Integrating molecular genetics with plant breeding to deliver impact

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-03-04 DOI: 10.1093/plphys/kiaf087

Rajiv Sharma, Chin Jian Yang, Nicola Rossi, Emma Irving, Ashley Tuffin, Hadi Aliki, Wayne Powell, Ian K Dawson

{"title":"Integrating molecular genetics with plant breeding to deliver impact","authors":"Rajiv Sharma, Chin Jian Yang, Nicola Rossi, Emma Irving, Ashley Tuffin, Hadi Aliki, Wayne Powell, Ian K Dawson","doi":"10.1093/plphys/kiaf087","DOIUrl":"https://doi.org/10.1093/plphys/kiaf087","url":null,"abstract":"Summary The appropriate use of molecular genetic approaches to reach impact in plant breeding is not straightforward. Here, we consider theory and realised application, and explore key issues that early-career plant molecular genetic researchers especially should consider as they establish their careers, particularly in relation to opportunities in plant breeding that span academia and the private sector. Useful entry points for these researchers are an understanding of the plant breeding cycle and the breeder’s equation. Becoming familiar with success and failure factors in the application of molecular genetics to practical plant breeding, including in relation to quantitative genetic principles, is also important. Other framing issues are scenario modelling for choosing between breeding scheme options, how agronomy and plant breeding interrelate, and the needs of future food production systems. We also recommend that early-career plant molecular genetic researchers look at whether pathways have been mapped out for how research will lead to impact at field level, whether stakeholders’ perspectives have been accounted for, and whether the effectiveness of molecular genetic versus alternative interventions have been costed. Early-career researchers should also consider if effective systems are in place to monitor the values of molecular interventions, and whether the necessary multidisciplinary teams are involved in crop development and deployment. We believe that through building a cadre of well-informed and well-connected early-career plant molecular genetic researchers, transformational change in the applications of molecular genetics to practical plant breeding will be enhanced.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"35 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143546245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolites managing excess manganese: the SgPAL2-regulated flavonoids in stylo.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-02-25 DOI: 10.1093/plphys/kiaf062

Munkhtsetseg Tsednee

引用次数: 0

Water-saving GC-MC model captures temporally differential enzymatic and transporter activities during C3-CAM transition.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-02-21 DOI: 10.1093/plphys/kiaf048

Devlina Sarkar, Sudip Kundu

引用次数: 0

Viral delivery of recombinases activates heritable genetic switches in plants.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-02-21 DOI: 10.1093/plphys/kiaf073

James C Chamness, Jon P Cody, Anna J Cruz, Daniel F Voytas

{"title":"Viral delivery of recombinases activates heritable genetic switches in plants.","authors":"James C Chamness, Jon P Cody, Anna J Cruz, Daniel F Voytas","doi":"10.1093/plphys/kiaf073","DOIUrl":"https://doi.org/10.1093/plphys/kiaf073","url":null,"abstract":"Viral vectors provide an increasingly versatile platform for transformation-free reagent delivery to plants. RNA viral vectors can be used to induce gene silencing, overexpress proteins, or introduce gene editing reagents; however, they are often constrained by carrying capacity or restricted tropism in germline cells. Site-specific recombinases that catalyze precise genetic rearrangements are powerful tools for genome engineering that vary in size and, potentially, efficacy in plants. In this work, we show that viral vectors based on tobacco rattle virus (TRV) deliver and stably express four recombinases ranging in size from ∼0.6kb to ∼1.5kb and achieve simultaneous marker removal and reporter activation through targeted excision in transgenic Nicotiana benthamiana lines. TRV vectors with Cre, FLP, CinH, and Integrase13 efficiently mediated recombination in infected somatic tissue and led to heritable modifications at high frequency. An excision-activated Ruby reporter enabled simple and high-resolution tracing of infected cell lineages without the need for molecular genotyping. Together, our experiments broaden the scope of viral recombinase delivery and offer insights into infection dynamics that may be useful in developing future viral vectors.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

S-RNase Evolution in Self-Incompatibility: Phylogenomic Insights into Synteny with Class I T2 RNase Genes

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf072

Yunxiao Liu, Yangxin Zhang, Songxue Han, Bocheng Guo, Jiakai Liang, Ze Yu, Fan Yang, Yaqiang Sun, Jiayu Xue, Zongcheng Lin, M Eric Schranz, Changfei Guan, Fengwang Ma, Tao Zhao

{"title":"S-RNase Evolution in Self-Incompatibility: Phylogenomic Insights into Synteny with Class I T2 RNase Genes","authors":"Yunxiao Liu, Yangxin Zhang, Songxue Han, Bocheng Guo, Jiakai Liang, Ze Yu, Fan Yang, Yaqiang Sun, Jiayu Xue, Zongcheng Lin, M Eric Schranz, Changfei Guan, Fengwang Ma, Tao Zhao","doi":"10.1093/plphys/kiaf072","DOIUrl":"https://doi.org/10.1093/plphys/kiaf072","url":null,"abstract":"S-RNases are essential in the gametophytic self-incompatibility (GSI) system of many flowering plants, where they act as stylar-S determinants. Despite their prominence, the syntenic genomic origin and evolutionary trajectory of S-RNase genes in eudicots have remained largely unclear. Here, we performed large-scale phylogenetic and microsynteny network analyses of T2 RNase genes across 130 angiosperm genomes, encompassing 35 orders and 56 families. S-like RNase genes in Cucurbitaceae species phylogenetically grouped with functionally characterized S-RNases in various species. Additionally, Cucurbitaceae S-like RNase genes showed conserved synteny with Class I T2 RNase genes. From this, we inferred that the well-characterized S-RNase genes (belonging to Class III-A genes) and Class I T2 RNase genes (located on duplicated genomic blocks) likely derived from the gamma triplication event shared by core eudicots. Additionally, we identified frequent lineage-specific gene transpositions of S-RNases and S-like RNases across diverse angiosperm lineages, including Rosaceae, Solanaceae, and Rutaceae families, accompanied by a significant increase in transposable element (TE) activity near these genes. Our findings delineate the genomic origin and evolutionary path of eudicot S-RNase genes, enhancing our understanding of the evolution of the S-RNase-based GSI system.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"50 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The parsley genome assembly and DNA methylome shed light on apigenin biosynthesis in the Apiaceae

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf077

Hui Liu, Jia-Qi Zhang, Chen Chen, Ya-Hui Wang, Zhi-Sheng Xu, Qin-Zheng Zhao, Jian Zhang, Jia-Yu Xue, Ai-Sheng Xiong

{"title":"The parsley genome assembly and DNA methylome shed light on apigenin biosynthesis in the Apiaceae","authors":"Hui Liu, Jia-Qi Zhang, Chen Chen, Ya-Hui Wang, Zhi-Sheng Xu, Qin-Zheng Zhao, Jian Zhang, Jia-Yu Xue, Ai-Sheng Xiong","doi":"10.1093/plphys/kiaf077","DOIUrl":"https://doi.org/10.1093/plphys/kiaf077","url":null,"abstract":"Parsley (Petroselinum crispum (Mill.)) is a medicinal and edible vegetable of the Apiaceae family that is rich in apigenin. The Apiaceae family is well known for its diverse secondary metabolites. As a high-quality reference genome is lacking for parsley, the evolution and apigenin biosynthesis in Apiaceae have remained unexplored. Here, we report the chromosome-level genome sequence of parsley, consisting of 1.85 Gb that mainly arose from the expansion of long terminal repeats. Whole-genome bisulfite sequencing (WGBS) revealed a significantly higher number of hypermethylated differentially expressed genes (hyper-DMGs) in leaf blades and petioles than in root tissues. Moreover, we identified and characterized chalcone isomerase (CHI) genes, encoding key enzymes involved in apigenin biosynthesis in parsley. We also established that the APETALA2 family transcription factor Pcrispum_6.2855 (PcAP2) binds to the (Pcrispum_11.4764) PcCHI promoter and promotes apigenin accumulation. In conclusion, our work presents a multi-omics data resource for understanding apigenin biosynthesis and its transcriptional regulation in parsley, in addition to shedding light on the evolution of parsley within the Apiaceae.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"25 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jasmonic acid and nitric oxide orchestrate a hierarchical melatonin cascade for Botrytis cinerea resistance in tomato

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf078

Qing Shan, Dan Zhao, Bili Cao, Xueying Zhu, Chengqiang Wang, Lei Deng, Chuanyou Li, Yang Zhang, Qinghua Shi, Biao Gong

{"title":"Jasmonic acid and nitric oxide orchestrate a hierarchical melatonin cascade for Botrytis cinerea resistance in tomato","authors":"Qing Shan, Dan Zhao, Bili Cao, Xueying Zhu, Chengqiang Wang, Lei Deng, Chuanyou Li, Yang Zhang, Qinghua Shi, Biao Gong","doi":"10.1093/plphys/kiaf078","DOIUrl":"https://doi.org/10.1093/plphys/kiaf078","url":null,"abstract":"Tomato gray mold, caused by Botrytis cinerea (B. cinerea), poses a major challenge to tomato production. In plants, B. cinerea resistance is positively regulated by melatonin; however, the regulatory mechanism of melatonin biosynthesis during B. cinerea infection is not known. Here, we established the working model of jasmonic acid (JA) and nitric oxide (NO) on melatonin biosynthesis in the state of B. cinerea infection. NO responded to B. cinerea infection earlier than JA. In the early stage of infection, tomato caffeic acid O-methyltransferase 2 (SlCOMT2) was S-nitrosylated by NO at Cys344, enhancing the stability of SlCOMT2 and preventing its degradation via the 26S proteasome. In the late stage of infection, JA-triggered SlMYC2 bound to SlCOMT1 and SlCOMT2 promoters for their transcription. NO and JA synergistically enhanced COMT-mediated melatonin biosynthesis during B. cinerea infection via post-translational modification and transcriptional activation. The accumulation of melatonin in tomato leaves inhibited cell death by scavenging reactive oxygen species (ROS), thereby preventing B. cinerea from establishing infection sites. We propose that SlCOMT2Cys344 is a genetic manipulation site or biological breeding target that can be used to enhance melatonin synthesis and B. cinerea resistance in tomato.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"3 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factors AdNAC3 and AdMYB19 regulate kiwifruit ripening through brassinosteroid and ethylene signaling networks

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf084

Yaming Yang, Ming Chen, Qinggang Zhu, Yanrong Lv, Cuihua Liu, Yun Wei, Guili Cha, Xiaoyan Shi, Xiaolin Ren, Yuduan Ding

{"title":"The transcription factors AdNAC3 and AdMYB19 regulate kiwifruit ripening through brassinosteroid and ethylene signaling networks","authors":"Yaming Yang, Ming Chen, Qinggang Zhu, Yanrong Lv, Cuihua Liu, Yun Wei, Guili Cha, Xiaoyan Shi, Xiaolin Ren, Yuduan Ding","doi":"10.1093/plphys/kiaf084","DOIUrl":"https://doi.org/10.1093/plphys/kiaf084","url":null,"abstract":"The pivotal role of ethylene (ETH) in fruit ripening has been extensively studied; however, the function of brassinosteroids (BRs) in regulating fruit ripening remains poorly understood. Specifically, the mechanism by which BRs interact with ETH to affect kiwifruit (Actinidia deliciosa) ripening is unclear. Our research showed that two genes encoding transcription factors, AdNAC3 and AdMYB19, and the fruit softening gene AdEXP3 (encoding a cell wall expansion protein, expansin 3) were upregulated by ETH and downregulated by BRs. Furthermore, AdNAC3 and AdMYB19 positively regulated the activity of the AdEXP3 promoter, and AdNAC3 positively regulated the promoter activity of AdMYB19. The physical interaction between AdNAC3 and the B-box-type zinc finger protein AdBBX32 affected fruit ripening. Transient overexpression and silencing experiments revealed that ETH upregulated and BRs downregulated the expression of AdNAC3 and AdMYB19, thereby regulating the expression level of AdEXP3 and participating in pectin degradation. Stable transformation of AdNAC3 in tomato fruits accelerated fruit color change and promoted fruit ripening. These results indicate that AdNAC3 and AdMYB19 are involved in the hormone interaction between BRs and ETH in regulating kiwifruit ripening, providing insights into the molecular mechanisms underlying the crosstalk between BRs and ETH.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"15 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A powdery mildew core effector protein targets the host endosome tethering complexes HOPS and CORVET in barley.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf067

Björn Sabelleck, Sohini Deb, Sophie C J Levecque, Matthias Freh, Anja Reinstädler, Pietro D Spanu, Hans Thordal-Christensen, Ralph Panstruga

{"title":"A powdery mildew core effector protein targets the host endosome tethering complexes HOPS and CORVET in barley.","authors":"Björn Sabelleck, Sohini Deb, Sophie C J Levecque, Matthias Freh, Anja Reinstädler, Pietro D Spanu, Hans Thordal-Christensen, Ralph Panstruga","doi":"10.1093/plphys/kiaf067","DOIUrl":"https://doi.org/10.1093/plphys/kiaf067","url":null,"abstract":"Powdery mildew fungi are serious pathogens affecting many plant species. Their genomes encode extensive repertoires of secreted effector proteins that suppress host immunity. Here, we revised and analyzed the candidate secreted effector protein (CSEP) effectome of the powdery mildew fungus, Blumeria hordei (Bh). We identified seven putative effectors that are broadly conserved in powdery mildew species, suggesting that they are core effectors of these phytopathogens. We showed that one of these effectors, CSEP0214, interacts with the barley (Hordeum vulgare) vacuolar protein sorting 18 (VPS18) protein, a shared component of the class C core vacuole/endosome tethering (CORVET) and homotypic fusion and protein-sorting (HOPS) endosomal tethering complexes that mediate fusion of early endosomes and multivesicular bodies, respectively, with the central vacuole. Overexpression of CSEP0214 and knockdown of either VPS18, HOPS-specific VPS41 or CORVET-specific VPS8 blocked the vacuolar pathway and the accumulation of the fluorescent vacuolar marker protein (SP)-RFP-AFVY in the endoplasmic reticulum. Moreover, CSEP0214 inhibited the interaction between VPS18 and VPS16, which are both shared components of CORVET as well as HOPS. Additionally, introducing CSEP0214 into barley leaf cells blocked the hypersensitive cell death response associated with resistance gene-mediated immunity, indicating that endomembrane trafficking is required for this process. CSEP0214 expression also prevented callose deposition in cell wall appositions at attack sites and encasements of fungal infection structures. Our results indicate that the powdery mildew core effector CSEP0214 is an essential suppressor of plant immunity.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0