Plant Physiology最新文献

The hyccin-containing protein ECDR1 interacts with PI3K1 and PI4K1 and regulates broad-spectrum disease resistance in rice 含有葫芦素的蛋白ECDR1与PI3K1和PI4K1相互作用，调控水稻的广谱抗病性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-05 DOI: 10.1093/plphys/kiag663

Shuang Zhang,Lingzhi Meng,Zhichao Zhao,Changyan Qi,Chen Xie,Xiaokang Jiang,Ling Jiang,Yulong Ren,Zhijun Cheng,Jie Wang,Qibing Lin,Xiuping Guo,Xin Wang,Shanshan Zhu,Cailin Lei,Jianmin Wan

{"title":"The hyccin-containing protein ECDR1 interacts with PI3K1 and PI4K1 and regulates broad-spectrum disease resistance in rice","authors":"Shuang Zhang,Lingzhi Meng,Zhichao Zhao,Changyan Qi,Chen Xie,Xiaokang Jiang,Ling Jiang,Yulong Ren,Zhijun Cheng,Jie Wang,Qibing Lin,Xiuping Guo,Xin Wang,Shanshan Zhu,Cailin Lei,Jianmin Wan","doi":"10.1093/plphys/kiag663","DOIUrl":"https://doi.org/10.1093/plphys/kiag663","url":null,"abstract":"Abstract Programmed cell death and immunity in plants are finely orchestrated to promote antimicrobial defense while preventing autoimmunity. However, the molecular mechanisms involved are not fully understood. Here, we isolated a rice mutant ecdr1 (enhanced cell death and resistance 1) that displayed an autoimmunity phenotype and enhanced resistance to rice blast and bacterial blight, and identified ECDR1 as a new shared component in PI3K and PI4K complexes, linking the hyccin-containing protein with plant defense responses. ECDR1 was expressed at all developmental stages and in all tissues examined. The ECDR1 was highly conserved in function across monocots and dicots. The 113 bp deletion in the ECDR1 promoter reduced its expression, leading to cell death and enhanced disease resistance. The ECDR1 was localized in plasma membrane, and interacted with TPR1 and TPR2 which in turn interacted with both PI4K1 and PI3K1, suggesting that ECDR1 could function as a component associated with not only PI4K complexes but also PI3K complexes to help catalyze PI into PI3P and PI4P. The lethality of all homozygous ecdr1, pi3k1 and tpr1 tpr2 mutants indicated the crucial roles of these genes in plant normal growth, which restricted our understanding of PI3K and PI4K functions. Alternatively, exogenous application of PI3K and PI4K inhibitors could substantially exacerbate the cell death and enhance disease resistance, implying their roles in plant immunity. Our findings provide novel insights into the regulatory mechanisms of ECDR1 in cell death and defense pathways, will aid in understanding the functions of PI3K and PI4K in plant immunity.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"147 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148894516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining timelapse imaging resolved leaf water potential with gas exchange provides insights into stomatal control by water 结合时移成像解决了叶片水势与气体交换，提供了对水分控制气孔的见解

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-05 DOI: 10.1093/plphys/kiag660

Anju Manandhar,Ian M Rimer,Scott A M McAdam

{"title":"Combining timelapse imaging resolved leaf water potential with gas exchange provides insights into stomatal control by water","authors":"Anju Manandhar,Ian M Rimer,Scott A M McAdam","doi":"10.1093/plphys/kiag660","DOIUrl":"https://doi.org/10.1093/plphys/kiag660","url":null,"abstract":"Abstract Stomatal responses to changes in leaf water status are amongst the most important responses of guard cells for balancing carbon gain with water loss, yet we do not know if guard cells are hydraulically linked to the water status of the leaf across vascular land plants. We combined time-lapsing imaging of leaf tissue in the cuvette of a gas analyzer to provide a dynamic measurement of leaf water potential (Ψl) while simultaneously recording leaf gas exchange to test these questions. Using this method in a representative lycophyte Selaginella moellendorfii we show that stomata respond passively to changes in Ψl by closing as soon as Ψl declines and opening rapidly on rehydration, confirming a close hydraulic connection between guard cells and the leaf in lycophytes. In contrast, in a representative angiosperm Solanum americanum, wrong-way stomatal responses occurred when leaves experienced rapid changes in Ψl. In this species wrong-way responses no longer occurred once Ψl had declined to turgor loss point, at which point stomata will rapidly reopen on rehydration without a wrong-way response. These observations confirm a close hydraulic connection between the guard cell cytosol and leaf apoplast across vascular land plants and rule out a hypothesis that there is a rapid and transient rehydration of leaf tissue from embolized xylem on leaf excision, as an explanation for wrong-way stomatal opening. The ability to simultaneously record leaf gas exchange and Ψl provides a powerful tool for resolving outstanding questions related to the hydraulic regulation of stomatal aperture.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"48 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148894517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Xanthomonas effector increases virulence quantitatively by inducing at least three minor susceptibility genes 黄单胞菌效应物通过诱导至少三个次要的易感基因来定量地增加毒力

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-05 DOI: 10.1093/plphys/kiag665

Brice Charleux,Carine Gris,Sébastien Carrère,Alvaro L Pérez-Quintero,Aurélie Le Ru,Caroline Bellenot,Ivanna Fuentes,Zoë E Dubrow,Adam J Bogdanove,Laurent D Noël,Corinne Audran

{"title":"A\u0000 Xanthomonas\u0000 effector increases virulence quantitatively by inducing at least three minor susceptibility genes","authors":"Brice Charleux,Carine Gris,Sébastien Carrère,Alvaro L Pérez-Quintero,Aurélie Le Ru,Caroline Bellenot,Ivanna Fuentes,Zoë E Dubrow,Adam J Bogdanove,Laurent D Noël,Corinne Audran","doi":"10.1093/plphys/kiag665","DOIUrl":"https://doi.org/10.1093/plphys/kiag665","url":null,"abstract":"The transcription activator-like effector Tal12a is widely conserved among Xanthomonas campestris pv. campestris strains that cause black rot in Brassica crops. However, its role in disease remains unclear. To investigate how Tal12a contributes to pathogenesis, we combined transcriptomic profiling of cauliflower leaves infected with Tal12a-expressing strains, prediction of TALE-binding elements, and heterologous expression assays in Nicotiana benthamiana. The aim of this approach was to identify candidate susceptibility genes. Artificial TALEs were further employed to validate the contribution of these candidate targets to disease development. Tal12a enhanced both virulence and bacterial growth in cauliflower. Transcriptome analysis revealed 380 genes that were induced upon infection, nine of which were prioritised as candidates. Seven of these were confirmed as direct Tal12a targets, while the induction of the sugar transporter genes BoSWEET13 and BoSWEET14c likely occurred indirectly. Functional assays demonstrated that these two SWEET genes and the BoIAA7c gene, which encodes auxin-dependent transcriptional regulator, contribute to disease development. These findings identify the first susceptibility genes in cauliflower and reveal that Tal12a promotes disease through a complex transcriptional reprogramming, involving direct and indirect target induction, with several genes functioning as minor susceptibility factors.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"175 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148894511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoplastic and nickel nanoparticles modulate soybean performance and nitrogen assimilation 纳米塑料和镍纳米颗粒调节大豆生产性能和氮同化

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-05 DOI: 10.1093/plphys/kiag651

Imran Azeem,Muhammad Adeel,Noman Shakoor,Adnan Anwar khan,Muhmmad Zain,Jawad Ullah,Muhmmad Nadeem,Kamran Azeem,Rui Yukui

{"title":"Nanoplastic and nickel nanoparticles modulate soybean performance and nitrogen assimilation","authors":"Imran Azeem,Muhammad Adeel,Noman Shakoor,Adnan Anwar khan,Muhmmad Zain,Jawad Ullah,Muhmmad Nadeem,Kamran Azeem,Rui Yukui","doi":"10.1093/plphys/kiag651","DOIUrl":"https://doi.org/10.1093/plphys/kiag651","url":null,"abstract":"Abstract The increasing release of nanoplastics (NPx) and engineered nanoparticles (NPs) into terrestrial ecosystems poses emerging risks to soil biota and the food chain. The effects of NPx contamination on soybean growth and N2 fixation potential in the presence of nickel oxide nanoparticles (NiO-NPs) remain poorly understood. This study investigated the accumulation and phytotoxicity of polystyrene nanoplastic (PS NPx) and NiO-NPs in soybean and their effects on nitrogen fixation potential. Single or co-application of NPx and NiO-NPs (100 mg kg-1) markedly reduced photosynthetic efficiency (8-48%), elevated biochemical stress markers (25-164%), downregulated phytohormone levels (13-54%), and negatively affected nitrogen fixation potential (19-35%), ultimately leading to reduced plant physiological growth (21-57%). This was due to PS-NPx accumulation in the leaves and structural abnormalities in vacuoles (including altered vacuole morphology), cell walls, mitochondria, and chloroplasts. In contrast, NiO-NPs at 50 mg kg-1 significantly increased nodule formation (16%) and biomass (20%), thereby enhancing N2-fixation (14%) in soybean plants. This improvement was attributed to the upregulation of N2 assimilation enzymes in nodules, roots, and shoots that promoted N2 content in the plant, resulting in increased plant growth. This study improves our understanding of the potential environmental risks associated with the accumulation of NPx and NiO-NPs in legumes crops and provides valuable scientific insights into their impacts on plant growth and N2 fixation in soybean.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"11 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148894512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bypassing the Bottleneck: Broad-host geminivirus enables systemic, tissue-culture-free plant genome editing 绕过瓶颈：广泛宿主双病毒实现系统的，无组织培养的植物基因组编辑

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-05 DOI: 10.1093/plphys/kiag652

Ved Prakash,Praveen Khatri

引用次数: 0

Phytic acid accumulation by phosphorus acquisition contributes to rice defense against Bipolaris oryzae infection 植酸积累对水稻抵御稻瘟病有重要作用

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-05 DOI: 10.1093/plphys/kiag661

Qing Yuan,Wenyan Li,Juan Li,Bing Zhou,Fei Wang,Lingfei Xue,Ying Zhou,Yingmin Wang,Hua Shi,Huichuan Huang,Youyong Zhu,Yongsheng Zhu,Mo Wang

引用次数: 0

Methylosome and SMN complexes are dispensable for plant viability in Arabidopsis thaliana 甲基体和SMN复合物对拟南芥植物的生存能力是必不可少的

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-05 DOI: 10.1093/plphys/kiag667

Daniela Goretti,Silvio Collani,Sarah Muniz Nardeli,Hemamshu Ratnakaram,Stéphanie Robert,Markus Schmid

{"title":"Methylosome and SMN complexes are dispensable for plant viability in\u0000 Arabidopsis thaliana","authors":"Daniela Goretti,Silvio Collani,Sarah Muniz Nardeli,Hemamshu Ratnakaram,Stéphanie Robert,Markus Schmid","doi":"10.1093/plphys/kiag667","DOIUrl":"https://doi.org/10.1093/plphys/kiag667","url":null,"abstract":"Abstract The role of RNA splicing as a modulator of the molecular responses to stress is well described. In contrast, its importance in the acclimation of plants to changes in ambient temperatures has only recently started to emerge. Here, we analyzed the role of temperature in regulating the functionality of factors associated with snRNP biogenesis, a key process underlying pre-mRNA splicing. Taking advantage of mutants showing temperature-dependent phenotypes, we conducted a comprehensive study of the role that the methylosome and SMN complexes have in plant development. Genetic, phylogenetic, and confocal analyses, as well as in vivo and in vitro evidence, reveal remarkable differences in the composition and importance of these complexes between plants and vertebrate animals. The SMN complex in Arabidopsis is apparently reduced to a single protein, GEMIN2, that is not essential for plant development, and the existence of a SMN ortholog is uncertain. Similarly, components of the methylosome previously implicated in snRNP biogenesis are not essential for plant viability. Our results suggest that factors considered central to snRNP biogenesis in animals have less crucial roles in plants and highlight how an evolutionarily conserved molecular process like RNA splicing has nevertheless evolved plant specific characteristics.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"18 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148894515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guard cell Signal Transduction Mechanisms Controlling Stomatal Movements by Light, Abscisic Acid, CO2, VPD and Heat 光、脱落酸、CO2、VPD和热控制气孔运动的保护细胞信号转导机制

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-04 DOI: 10.1093/plphys/kiag653

Julian I Schroeder, Toshinori Kinoshita, Nobuyuki Uozumi, Sarah M Assmann

{"title":"Guard cell Signal Transduction Mechanisms Controlling Stomatal Movements by Light, Abscisic Acid, CO2, VPD and Heat","authors":"Julian I Schroeder, Toshinori Kinoshita, Nobuyuki Uozumi, Sarah M Assmann","doi":"10.1093/plphys/kiag653","DOIUrl":"https://doi.org/10.1093/plphys/kiag653","url":null,"abstract":"Guard cell pairs form dynamic stomatal gas exchange pores in aerial tissues of plants. Guard cells regulate CO2 influx and water loss through stomata by integrating multiple environmental and internal signals. Guard cells serve as highly specialized sensory and signal processing cells, thereby orchestrating stomatal movements in response to changing conditions. This review describes recent advances and early discoveries in elucidation of guard cell signaling networks. Present day understanding of the molecular and cellular mechanisms mediating stomatal opening by blue light, red light, heat stress and photosynthesis-mediated low CO2 in intercellular leaf spaces are reviewed. Furthermore, up-to-date knowledge of the molecular mechanisms and pathways that trigger stomatal closing by abscisic acid (ABA), elevated CO2 and low humidity/high vapor pressure difference is synthesized. These pathways promote stomatal closure and water conservation. Critical functions of other tissues are described, including mesophyll cells as producers of messengers for red light-induced stomatal opening and vascular tissues in drought-induced ABA synthesis and transport. Guard cell ion channels and pumps drive the ion fluxes required for the cellular osmotic motor that opens and closes stomatal pores. Roles of and recent advances toward understanding the regulatory mechanisms of these membrane transporters as downstream targets of guard cell signaling cascades are described. Synthesis of these signaling cascades into signaling networks is reviewed and open questions for future research are highlighted throughout. Understanding these integrated mechanisms is critical for future improvement of plant water use efficiency and crop environmental stress resilience.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"10 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148883513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear transfer of a beneficial fungal effector-host target complex via the NTF2 pathway underpins symbiont-induced plant immunity 通过NTF2途径进行有益真菌效应器-宿主靶复合物的核转移是共生体诱导的植物免疫的基础

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-04 DOI: 10.1093/plphys/kiag654

Yingqi Zhang, Zhuohua Liu, Yu Zhang, Ziran Yang, Yang Yang, Xueni Mai, Yitong Liang, Laura Rehneke, Yuling Meng, Patrick Schäfer, Weixing Shan

{"title":"Nuclear transfer of a beneficial fungal effector-host target complex via the NTF2 pathway underpins symbiont-induced plant immunity","authors":"Yingqi Zhang, Zhuohua Liu, Yu Zhang, Ziran Yang, Yang Yang, Xueni Mai, Yitong Liang, Laura Rehneke, Yuling Meng, Patrick Schäfer, Weixing Shan","doi":"10.1093/plphys/kiag654","DOIUrl":"https://doi.org/10.1093/plphys/kiag654","url":null,"abstract":"The symbiotic fungus Serendipita indica confers broad-spectrum beneficial effects on diverse plant hosts. Its key effector SIE141 elicits immunity against Phytophthora and salt tolerance by binding and relocalizing thioredoxin CDSP32 from chloroplast to the nucleus. Here, we show that this functionally essential nuclear transfer process of the SIE141-CDSP32 complex is mediated by the host NTF2 proteins. NTF2 family proteins are direct targets of SIE141, whose knockdown abolished nuclear accumulation of both SIE141 and CDSP32, leading to their rendered accumulation to chloroplasts. The glutamine residue at position 40 of NbNTF2A is critical for its interaction with both SIE141 and Ran GTPase 1. SIE141 modulates NbNTF2-NbRan1 interaction in a dose-dependent manner, without impairing nuclear accumulation of NbRan1 and the NTF2-mediated positive immune function of Ran1. Our results reveal a previously unknown mechanism where a beneficial symbiotic fungal effector utilizes the conserved NTF2 rather than other core nuclear import machinery components to traffic an immune complex and enhance disease resistance. These findings provide a potential strategy for engineering plant immunity by manipulating NTF2-RanGTPase dependent nucleocytoplasmic transport.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"35 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR160a-ARF4 module regulates the biosynthesis of Panax notoginseng saponins miR160a-ARF4模块调控三七皂苷的生物合成

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-03 DOI: 10.1093/plphys/kiag656

Wenqing Li, Chunyu Liu, Yuan Qu, Diqiu Liu, Feng Ge

{"title":"miR160a-ARF4 module regulates the biosynthesis of Panax notoginseng saponins","authors":"Wenqing Li, Chunyu Liu, Yuan Qu, Diqiu Liu, Feng Ge","doi":"10.1093/plphys/kiag656","DOIUrl":"https://doi.org/10.1093/plphys/kiag656","url":null,"abstract":"Panax notoginseng is an economically vital medicinal plant, prized for its bioactive P. notoginseng saponins (PNS) which possess significant therapeutic effects in cardiovascular and cerebrovascular diseases. Despite the elucidation of the core enzymatic steps in PNS biosynthesis, the post-transcriptional mechanisms that coordinate these metabolic fluxes remain largely obscure. This study explored the Pn-miR160a-ARF4 module as a pivotal regulation mode in the biosynthesis of PNS. Under methyl jasmonate (MeJA) stimulation, Pn-miR160a was significantly upregulated, subsequently targeting and cleaving the transcripts of auxin response factor 4 (PnARF4). This targeted silencing of PnARF4 effectively relieves its transcriptional repression, thereby promoting the expression of downstream biosynthetic genes and increasing PNS yield. By integrating hormonal signaling with miRNA-mediated gene silencing, these results uncover a sophisticated regulatory axis that governs specialized metabolism. Furthermore, this work expands the known functional scope of the evolutionarily conserved miR160-ARF module beyond its traditional role in plant architecture and auxin signaling, repositioning it as a central component of secondary metabolic regulation. These findings provide a theoretical foundation and a robust molecular target for the metabolic engineering and genetic improvement of P. notoginseng quality.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"22 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148877338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0