Plant Physiology最新文献

{"title":"Heat stress response and transposon control in plant shoot stem cells","authors":"Vu Hoang Nguyen, Ortrun Mittelsten Scheid, Ruben Gutzat","doi":"10.1093/plphys/kiaf110","DOIUrl":"https://doi.org/10.1093/plphys/kiaf110","url":null,"abstract":"Plants have an impressive repertoire to react to stress conditions that limit regular growth. Elevated temperatures beyond the optimal range cause rapid and specific transcriptional responses, resulting in developmental alterations and plasticity. Heat stress also causes chromatin decondensation and activation of some transposable elements (TEs), endangering genomic integrity. This is especially risky for stem cells in the shoot apical meristem (SAM) that potentially contribute to the next generation. We examined how the heat stress response in SAM stem cells of Arabidopsis (Arabidopsis thaliana) is different from that in other tissues and whether the elements of epigenetic TE control active in the meristem are involved in specific heat protection of stem cells. Using fluorescence-activated nuclear sorting to isolate and characterize SAM stem cells after exposure to conditions that activate a heat-responsive TE, we found SAM stem cells maintain their developmental program and suppress the heat response pathways dominating in surrounding somatic cells. Furthermore, mutants defective in DNA methylation recovered less efficiently from heat stress and persistently activated heat response factors and heat-responsive TEs. Heat stress also induced epimutations at the level of DNA methylation, especially in the CHG sequence context. Regions with modified DNA methylation patterns remained altered for at least three weeks beyond the stress. These findings urge for disentangling cell type-specific responses under different stress types, especially for stem cells as bridges to the next generation.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"33 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The OsIAA3-OsARF16-OsBUL1 auxin signaling module regulates grain size in rice.","authors":"Fengjun Xian, Shuya Liu, Jishuai Huang, Bin Xie, Lin Zhu, Qiannan Zhang, Chen Lv, Yimeng Xu, Xinrong Zhang, Jun Hu","doi":"10.1093/plphys/kiaf122","DOIUrl":"https://doi.org/10.1093/plphys/kiaf122","url":null,"abstract":"<p><p>Auxin plays an important role in various aspects of plant growth and development. However, the molecular mechanism underlying the control of grain size via auxin signaling pathways remains obscure. Here, we report that AUXIN/INDOLE-3-ACETIC ACID protein 3 (OsIAA3) positively regulates rice (Oryza sativa) grain size by promoting the cell expansion and proliferation of spikelet hulls. OsIAA3 interacted with 11 AUXIN RESPONSE FACTORS (ARFs), among which the interaction with OsARF16 was the strongest. The osarf16 knockout mutant showed smaller grains with decreased grain length, grain width, grain thickness, and 1,000-grain weight. Meanwhile, transgenic plants overexpressing OsARF16 produced noticeably larger grains with increased grain length and 1,000-grain weight. Oryza sativa BRASSINOSTEROID UPREGULATED 1-LIKE (OsBUL1), which encodes an atypical bHLH protein that positively regulates grain size by promoting cell expansion, is a direct target gene of OsARF16. The interaction between OsIAA3 and OsARF16 repressed the transcriptional activation of OsARF16 on OsBUL1. Our study reveals a OsIAA3-OsARF16-OsBUL1 module that regulates grain size, refining the molecular mechanism of the auxin signaling pathway involved in grain size control.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transcription factors MYB80 and TEK coordinate callose wall degradation and pollen exine formation in Arabidopsis.","authors":"Xiaofeng Xu, Kaiqi Wang, Yahui Yu, Xin Zhao, Yuyi Guo, Yaqi Liu, Xuexue Qian, Naiying Yang, Ping Xu, Zhong-Nan Yang","doi":"10.1093/plphys/kiaf124","DOIUrl":"https://doi.org/10.1093/plphys/kiaf124","url":null,"abstract":"<p><p>Pollen development involves cell wall alteration of the male gametophyte, which is critical for plant fertility and requires MYB80 and TRANSPOSABLE ELEMENT SILENCING VIA AT-HOOK (TEK) transcription factors in Arabidopsis (Arabidopsis thaliana). In this study, we found that the myb80 tek double mutant exhibits a compromised degradation of the tetrad callose wall and down-regulation of five ANTHER-SPECIFIC PROTEIN 6 (A6) genes encoding β-1,3-glucanase. The quintuple mutant of A6 (a6-quint) exhibited delayed callose wall degradation and defective exine structure, and its pollen had a weakened UV resistance. The quadruple mutant of A6 (a6-quad) restored the fertility of rvms-2, a thermo-sensitive genic male sterile (TGMS) line where the transition from tetrad wall to pollen wall is defective. Transgenic expression of A6 and A6.2 driven by the A9 promoter led to the expression of the two genes in the tapetum at earlier anther developmental stages, which caused premature callose wall dissolution and impeded exine formation, indicating the importance of the temporal control of A6s. Furthermore, dual-luciferase and ChIP assay results confirmed the direct regulation of MYB80 and TEK in activating the expression of the above A6s in the tapetum. In conclusion, callose degradation mediated by the MYB80/TEK-A6s pathway is required for the transition from tetrad callose wall to pollen wall.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The carboxylesterase AtCXE12 converts volatile (Z)-3-hexenyl acetate to (Z)-3-hexenol in Arabidopsis leaves.","authors":"Tristan M Cofer, James H Tumlinson","doi":"10.1093/plphys/kiaf119","DOIUrl":"https://doi.org/10.1093/plphys/kiaf119","url":null,"abstract":"<p><p>The green leaf volatiles (GLVs) (Z)-3-hexenal, (Z)-3-hexenol, and (Z)-3-hexenyl acetate play important roles in plant defense, deterring insect herbivores and attracting their natural enemies, while also serving as airborne signaling molecules capable of enhancing defenses in undamaged plant tissues. Almost all plants produce GLVs after wounding, beginning with the formation of (Z)-3-hexenal, which is subsequently converted to (Z)-3-hexenol and (Z)-3-hexenyl acetate. (Z)-3-hexenyl acetate can then be taken up by nearby plant tissues where it is predicted to be hydrolyzed to (Z)-3-hexenol, a process that is likely to be important in regulating the specific activities of these compounds. However, the enzyme(s) involved in this process and its role in plant defense are largely unknown. Here, we show that Arabidopsis (Arabidopsis thaliana) plants rapidly take up (Z)-3-hexenyl acetate and convert it to (Z)-3-hexenol. Inhibitor and fractionation experiments identified the carboxylesterases Carboxylesterase 5 (AtCXE5) and Carboxylesterase 12 (AtCXE12) as likely contributors to the (Z)-3-hexenyl acetate esterase activity in Arabidopsis leaves. Heterologous expression of AtCXE5 and AtCXE12 in Escherichia colirevealed that both recombinant enzymes hydrolyze (Z)-3-hexenyl acetate to (Z)-3-hexenol. Furthermore, assays using T-DNA insertion mutants showed that AtCXE12 significantly contributes to (Z)-3-hexenyl acetate hydrolysis in Arabidopsis. Lastly, we found that leaves from several other plant species possess (Z)-3-hexenyl acetate esterase activity, suggesting a conserved mechanism for GLV metabolism among plants. Overall, our study provides a better understanding of the biosynthesis and conversion dynamics of GLVs, which is necessary for unraveling the potential functions of these compounds.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutations in HEADING DATE 1 affect transcription and cell wall composition in rice.","authors":"Marco Biancucci, Daniele Chirivì, Alessio Baldini, Eugene Badenhorst, Fabio Dobetti, Bahman Khahani, Elide Formentin, Tenai Eguen, Franziska Turck, John P Moore, Elahe Tavakol, Stephan Wenkel, Fiorella Lo Schiavo, Ignacio Ezquer, Vittoria Brambilla, David Horner, Matteo Chiara, Giorgio Perrella, Camilla Betti, Fabio Fornara","doi":"10.1093/plphys/kiaf120","DOIUrl":"https://doi.org/10.1093/plphys/kiaf120","url":null,"abstract":"<p><p>Plants utilize environmental information to modify their developmental trajectories for optimal survival and reproduction. Over a century ago, day length (photoperiod) was identified as a major factor influencing developmental transitions, particularly the shift from vegetative to reproductive growth. In rice (Oryza sativa), exposure to day lengths shorter than a critical threshold accelerates flowering, while longer days inhibit this process. This response is mediated by HEADING DATE 1 (Hd1), a zinc finger transcription factor that is central in the photoperiodic flowering network. Hd1 acts as a repressor of flowering under long days but functions as a promoter of flowering under short days. However, how global transcription of genes downstream of Hd1 changes in response to the photoperiod is still not fully understood. Furthermore, it is unclear whether Hd1 target genes are solely involved in flowering time control or mediate additional functions. In this study, we utilized RNA-Seq to analyze the transcriptome of hd1 mutants under both long and short day conditions. We identified genes involved in the phenylpropanoid pathway that are deregulated under long days in the mutant. Quantitative profiling of cell wall components and abiotic stress assays suggested that Hd1 is involved in processes considered unrelated to flowering control. This indicates that day length perception and responses are intertwined with physiological processes beyond flowering.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHOTOPERIOD 1 enhances stress resistance and energy metabolism to promote spike fertility in barley under high ambient temperatures.","authors":"Tianyu Lan, Agatha Walla, Kumsal Ecem Çolpan Karışan, Gabriele Buchmann, Vera Wewer, Sabine Metzger, Isaia Vardanega, Einar Baldvin Haraldsson, Gesa Helmsorig, Venkatasubbu Thirulogachandar, Rüdiger Simon, Maria von Korff","doi":"10.1093/plphys/kiaf118","DOIUrl":"https://doi.org/10.1093/plphys/kiaf118","url":null,"abstract":"<p><p>High ambient temperature (HT) impairs reproductive development and grain yield in temperate crops. To ensure reproductive success under HT, plants must maintain developmental stability. However, the mechanisms integrating plant development and temperature resistance are largely unknown. Here, we demonstrate that PHOTOPERIOD 1 (PPD-H1), homologous to PSEUDO RESPONSE REGULATOR genes of the Arabidopsis (Arabidopsis thaliana) circadian clock, controls developmental stability in response to HT in barley (Hordeum vulgare). We analyzed the HT responses in independent introgression lines with either the ancestral wild-type Ppd-H1 allele or the natural ppd-h1 variant, selected in spring varieties to delay flowering and enhance yield under favorable conditions. HT delayed inflorescence development and reduced grain number in ppd-h1 mutant lines, while the wild-type Ppd-H1 genotypes exhibited accelerated reproductive development and showed a stable grain set under HT. Using a CRISPR/Cas9-induced ppd-h1 mutant, we confirmed that the CCT domain of Ppd-H1 controls developmental stability, but not clock gene expression. Transcriptome and phytohormone analyses in developing leaves and inflorescences revealed increased expression levels of stress-responsive genes and abscisic acid levels in the leaf and inflorescence of the natural and induced mutant ppd-h1 lines. Furthermore, the ppd-h1 lines displayed downregulated photosynthesis- and energy metabolism-related genes, as well as decreased auxin and cytokinin levels in the inflorescence, which impaired anther and pollen development. In contrast, the transcriptome, phytohormone levels, and anther and pollen development remained stable under HT in the wild-type Ppd-H1 plants. Our findings suggest that Ppd-H1 enhances stress resistance and energy metabolism, thereby stabilizing reproductive development, floret fertility and grain set under HT.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR164a-targeted NAM3 inhibits thermotolerance in tomato by regulating HSFA4b-mediated redox homeostasis.","authors":"Zelan Huang, Rui Lin, Yufei Dong, Mingjia Tang, Xiaojian Xia, Lei Fang, Jingquan Yu, Huijia Kang, Yanhong Zhou","doi":"10.1093/plphys/kiaf113","DOIUrl":"https://doi.org/10.1093/plphys/kiaf113","url":null,"abstract":"<p><p>Extreme weather events, including high temperatures, frequently occur and adversely affect crop growth, posing substantial challenges to global agriculture. MicroRNAs (miRNAs) play integral roles in regulating plant growth and responses to various stresses. In this study, we reveal that microRNA164a (miR164a) in tomato (Solanum lycopersicum) is a pivotal element that exhibits a rapid positive response to heat stress (HS) among multiple miRNAs, while its target NO APICAL MERISTEM 3 (NAM3) shows an opposite complementary response. MiR164a/b-5p-deficient mutant and NAM3-overexpressing plants resulted in increased sensitivity to HS, whereas mutants with reduced NAM3 levels exhibited enhanced thermotolerance. Importantly, HS-induced reactive oxygen species (ROS) accumulation and antioxidant enzyme activities were positively regulated by miR164a and negatively by NAM3, respectively. Furthermore, we demonstrated that NAM3 transcriptionally activated the expression of HSFA4b, and silencing HSFA4b improved tomato thermotolerance. HSFA4b repressed the expression of the antioxidant gene APX1 and the heat shock protein (HSP) gene HSP90, disrupting redox homeostasis and exacerbating oxidative stress. Our findings unveil a pivotal regulatory pathway governed by the miR164a-NAM3 module that confers thermotolerance in tomato via its influence on ROS-related and HSP pathways. These findings provide valuable insights into the molecular mechanisms that underpin tomato thermotolerance, which are crucial for advancing sustainable agricultural practices, particularly in the face of the challenges presented by global climate change.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TTLOC: A Tn5 transposase-based approach to localize T-DNA integration sites.","authors":"Xiao-Yuan Tao, Shou-Li Feng, Xin-Jia Li, Yan-Jun Li, Wei Wang, Matthew Gilliham, Zhong-Hua Chen, Sheng-Chun Xu","doi":"10.1093/plphys/kiaf102","DOIUrl":"https://doi.org/10.1093/plphys/kiaf102","url":null,"abstract":"<p><p>Thermal asymmetric interlaced (TAIL)-PCR-based and whole-genome sequencing-based T-DNA localization approaches have been developed for the recovery of T-DNA integration sites (TISs). Nevertheless, a low-cost and high-throughput technique for the detection of TISs, which would facilitate the identification of genetically engineered plants, is in high demand for rapid crop breeding and plant synthetic biology. Here, we present Tn5 transposase-based T-DNA Integration Site Localization (TTLOC), a Tn5-based approach for TIS localization. TTLOC employs specialized adaptor-assembled Tn5 transposases for genomic DNA tagmentation. TTLOC library construction is straightforward, involving only six steps that requires two and a half hours to complete. The resulting pooled library is compatible with next-generation sequencing, which enables high-throughput determination. We demonstrate the ability of TTLOC to recover 95 non-redundant TISs from 65 transgenic Arabidopsis (Arabidopsis thaliana) lines, and 37 non-redundant TISs from the genomes of transgenic rice (Oryza sativa), soybean (Glycine max), tomato (Solanum lycopersicum), potato (Solanum tuberosum), and from the large hexaploid wheat (Triticum aestivum) genome. TTLOC is a cost-effective method, as 1-2 Gb of raw data for each multiplexing library are sufficient for efficient TIS calling, independent of the genome size. Our results establish TTLOC as a promising strategy for evaluation of genome engineered plants, and for selecting genome safe harbors for trait stacking in crop breeding and plant synthetic biology.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Target of rapamycin signaling regulates starch degradation via α-glucan, water dikinase in a unicellular red alga.","authors":"Sota Komiya, Imran Pancha, Hiroki Shima, Kazuhiro Igarashi, Kan Tanaka, Sousuke Imamura","doi":"10.1093/plphys/kiaf106","DOIUrl":"https://doi.org/10.1093/plphys/kiaf106","url":null,"abstract":"<p><p>Target of rapamycin (TOR) signaling pathways are major regulators of starch accumulation in various eukaryotes. However, the underlying molecular mechanisms of this regulation remain elusive. Here, we report the role of TOR signaling in starch degradation in the unicellular red alga Cyanidioschyzon merolae. Reanalysis of our previously published phosphoproteome data showed that phosphorylation of the serine residue at position 264 of a protein similar to α-Glucan water dikinase (CmGWD), a key regulator of starch degradation, was not increased by rapamycin treatment. In the CmGWD knockout strain, starch content increased and starch phosphorylation decreased, indicating that CmGWD is a functional GWD. CmGWD-dependent starch degradation under dark conditions was alleviated by rapamycin treatment. The overexpression of a phosphomimic CmGWD variant, in which Ser264 was replaced by aspartic acid, or a dephosphomimic CmGWD variant, in which Ser264 was replaced by alanine, resulted in 0.6-fold lower and 1.6-fold higher starch accumulation compared to the wild-type CmGWD-overexpressing strain, respectively. The starch levels corresponded with starch phosphorylation status. Furthermore, the dephosphomimic CmGWD-overexpressing strain accumulated nearly the same amount of starch with or without rapamycin treatment as the rapamycin-treated wild-type CmGWD-overexpressing strain. In contrast, rapamycin treatment did not trigger an increase in starch accumulation in the phosphomimic CmGWD-overexpressing strain. These results indicate that TOR signaling regulates starch degradation in C. merolae by altering the phosphorylation state of Ser264 in CmGWD.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membranous translation platforms in the chloroplast of Chlamydomonas reinhardtii.","authors":"Yi Sun, Shiva Bakhtiari, Melissa Valente-Paterno, Heng Jiang, William Zerges","doi":"10.1093/plphys/kiaf111","DOIUrl":"https://doi.org/10.1093/plphys/kiaf111","url":null,"abstract":"<p><p>A small genome in chloroplasts encodes many of the polypeptide subunits of the photosynthetic electron transport complexes embedded in the membranes of thylakoid vesicles in the chloroplast stroma and synthesized by ribosomes of the bacterial-like genetic system of this semiautonomous organelle. While thylakoid membranes are sites of translation, evidence in the unicellular alga Chlamydomonas reinhardtii supports translation on non-canonical membranes in a discrete translation-zone in the chloroplast. To characterize the membranous platforms for translation and the biogenesis of thylakoid membranes, we profiled membranes during chloroplast development, using the yellow-in-the-dark1 mutant, and carried out proteomic analyses on two membrane types proposed previously to support translation in the chloroplast of C. reinhardtii: \"low-density membrane\" (LDM) and \"chloroplast translation membrane\" (CTM). The results support roles of LDM and CTM in preliminary and ongoing stages of translation, respectively. Proteomics, immunoprecipitation and transmission electron microscopy results support connections of these membranous platforms and a chloroplast envelope domain bound by cytoplasmic ribosomes. Our results contribute to a model of photosynthesis complex biogenesis in a spatiotemporal \"assembly line\" involving LDM and CTM as sequential stages leading to photosynthetic thylakoid membranes.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}