Plant Physiology最新文献

An old dog with new tricks – the value of photorespiration as a central metabolic hub with implications for environmental acclimation 老狗新把戏-光呼吸的价值作为一个中央代谢枢纽与环境适应的影响

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-14 DOI: 10.1093/plphys/kiaf258

Stefan Timm, Hu Sun, Martin Hagemann, Wei Huang, Alisdair R Fernie

{"title":"An old dog with new tricks – the value of photorespiration as a central metabolic hub with implications for environmental acclimation","authors":"Stefan Timm, Hu Sun, Martin Hagemann, Wei Huang, Alisdair R Fernie","doi":"10.1093/plphys/kiaf258","DOIUrl":"https://doi.org/10.1093/plphys/kiaf258","url":null,"abstract":"Photorespiration serves as a metabolic repair system that safeguards photosynthetic carbon fixation in photoautotrophic organisms thriving in today’s oxygen-rich atmosphere. This essential process detoxifies the inhibitory metabolite 2-phosphoglycolate (2PG), an unavoidable byproduct of ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) activity in the light. If not efficiently metabolized, 2PG impairs key enzymatic processes involved in carbon assimilation and utilization thereby inhibiting growth in oxygenic phototrophs. Decades of research have unraveled the biochemical and genetic intricacies of photorespiration, establishing it as the second-highest carbon flux in illuminated leaves. Here, we discuss recent developments that have expanded our understanding of the pathway, revealing novel metabolic players, intricate inter-organelle interactions, and new regulatory networks. Isotope labeling studies and reverse genetics have identified further interactions of the classical photorespiratory cycle with central carbon and nitrogen metabolism. In order to enhance photosynthetic efficiency, synthetic biology approaches have reengineered photorespiration, either by integrating bypass pathways or optimizing native enzymes. These interventions highlight the vast potential of optimized photorespiration to boost photosynthetic yield and enhance plant adaptation to future climates. Very recently, the importance of active photorespiration in guard cells was discovered, linking it to the regulation of stomatal metabolism and behavior. Collectively, these recent findings reinforce the immense promise of continued photorespiratory research in developing innovative strategies for improving plant yield and resilience.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"50 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of TaWRKY27 in the arms race between wheat and Stripe Rust. TaWRKY27在小麦和条锈病军备竞赛中的作用。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-06-14 DOI: 10.1093/plphys/kiaf259

Ritu Singh

引用次数: 0

Partners in light: Vitamin E and the price of being green. 光的伙伴：维生素E和绿色的代价。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-06-13 DOI: 10.1093/plphys/kiaf251

Thomas Depaepe

引用次数: 0

The transcription factor TaWRKY27 confers enhanced stripe rust susceptibility by facilitating auxin accumulation in wheat 转录因子TaWRKY27通过促进小麦生长素积累而增强小麦对条锈病的敏感性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-13 DOI: 10.1093/plphys/kiaf257

Mohan Yao, Zhen Liu, Yixin Qiao, Yuanfei Hou, Zhensheng Kang, Jie Liu

{"title":"The transcription factor TaWRKY27 confers enhanced stripe rust susceptibility by facilitating auxin accumulation in wheat","authors":"Mohan Yao, Zhen Liu, Yixin Qiao, Yuanfei Hou, Zhensheng Kang, Jie Liu","doi":"10.1093/plphys/kiaf257","DOIUrl":"https://doi.org/10.1093/plphys/kiaf257","url":null,"abstract":"Many pathogens exploit host susceptibility genes to facilitate their colonization. The disruption of these susceptibility genes holds the potential to confer broad-spectrum and robust disease resistance, offering a promising crop improvement strategy. However, little is known about the molecular mechanisms by which susceptibility genes suppress host immunity. In this study, we identified a transcription factor (TF) in wheat (Triticum aestivum L.), TaWRKY27, that was substantially induced during Puccinia striiformis f. sp. tritici (Pst) infection. TaWRKY27 is a nucleus-localized transcriptional activator. TaWRKY27 down-regulation in wheat conferred increased Pst resistance, while TaWRKY27 overexpression substantially decreased Pst resistance and increased auxin accumulation. Notably, auxin application to wheat leaves suppressed the expression of defense-related genes. Additionally, integrated DNA affinity purification sequencing (DAP-seq) and RNA-seq analyses demonstrated that TaWRKY27 directly activates the transcription of two 2-oxoglutarate-dependent dioxygenase (2OGD) superfamily members, aminocyclopropane carboxylate oxidase protein TaACO3 and senescence-related protein TaSRG1, a finding confirmed through yeast one-hybrid and dual-luciferase reporter assays. Notably, both TaACO3 and TaSRG1 negatively regulated wheat resistance to Pst. Taken together, our findings reveal that TaWRKY27 responds to Pst infection and contributes to wheat stripe rust susceptibility by facilitating TaACO3/TaSRG1-mediated auxin accumulation. These results highlight TaWRKY27 as a potential target for engineering enhanced stripe rust resistance in wheat.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"226 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The clock component LHY1b negatively regulates alkaline stress by repressing AOX1-mediated oxidative responses in soybean 时钟组分LHY1b通过抑制aox1介导的大豆氧化反应负向调节碱性胁迫

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-13 DOI: 10.1093/plphys/kiaf253

Jiaxian He, Jiayu Lin, Ning Wang, Jing Yang, Xucheng Yang, Yifei Chen, Manting Zhang, Ruifeng Chen, Xiulin Chen, Lidong Dong, Fanjiang Kong, Baohui Liu, Chunbao Zhang, Zhixi Tian, Meina Li

{"title":"The clock component LHY1b negatively regulates alkaline stress by repressing AOX1-mediated oxidative responses in soybean","authors":"Jiaxian He, Jiayu Lin, Ning Wang, Jing Yang, Xucheng Yang, Yifei Chen, Manting Zhang, Ruifeng Chen, Xiulin Chen, Lidong Dong, Fanjiang Kong, Baohui Liu, Chunbao Zhang, Zhixi Tian, Meina Li","doi":"10.1093/plphys/kiaf253","DOIUrl":"https://doi.org/10.1093/plphys/kiaf253","url":null,"abstract":"Increasing the ability of crop plants to maintain productivity in saline-alkaline lands is an absolute requirement for feeding the growing population. However, a shortage of knowledge about plant alkaline tolerance restricts breeding of salt-tolerant crops. Here we demonstrate that a homolog of the circadian clock component LATE ELONGATED HYPOCOTYL (LHY) negatively regulates reactive oxygen species (ROS) homeostasis and time-gates oxidative stress responses under NaHCO3 conditions in soybean (Glycine max). Yeast one-hybrid assays followed by chromatin immunoprecipitation coupled with quantitative PCR, electrophoretic mobility shift assays, and dual-luciferase reporter assays revealed that LHY1b binds directly to the promoter of Alternative Oxidase 1 (AOX1). Through immunoblotting using AOX1 antibody, we demonstrated that AOX1 accumulates considerably in lhy1b under NaHCO3 conditions. LHY1b strongly inhibits AOX1 transcription under normal growth conditions. However, this inhibition is removed under alkaline stress, allowing the expression of AOX1 and restricting the generation of ROS. Knockdown of AOX1 in lhy1b renders this mutant more sensitive to alkaline stress. Moreover, we determined that the naturally occurring LHY1bH2 allele is associated with alkaline tolerance by phenotyping 559 sequenced soybean accessions. Our study demonstrates a molecular link between clock components and alkaline tolerance and provides a valuable target gene for breeding alkaline-tolerant soybean varieties.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"37 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic insights into the domestication of tetraploid peanut 花生四倍体驯化的表观遗传学研究

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-13 DOI: 10.1093/plphys/kiaf254

Hang Yu, Chong Zhang, Chao Zhang, Yan Shi, Yajie Xue, Liang Xie, Haifeng Wang

{"title":"Epigenetic insights into the domestication of tetraploid peanut","authors":"Hang Yu, Chong Zhang, Chao Zhang, Yan Shi, Yajie Xue, Liang Xie, Haifeng Wang","doi":"10.1093/plphys/kiaf254","DOIUrl":"https://doi.org/10.1093/plphys/kiaf254","url":null,"abstract":"Polyploidization is a crucial evolutionary mechanism driving species domestication that promotes species formation and adaptation by providing additional genetic information to accelerate the functional differentiation of genes and evolution of new traits. Despite its importance as a key epigenetic modification, the role of DNA methylation in polyploid domestication through the regulation of gene expression remains unclear. Here, we performed whole genome bisulfite sequencing and RNA-seq analysis on the cultivated allotetraploid peanut (Arachis hypogaea L.) and its two ancestral diploids to investigate the epigenetic regulatory mechanisms of DNA methylation in the formation of peanut polyploids and in peanut domestication. Our findings unveiled substantial differences in DNA methylation between peanut subgenomes, particularly in non-CG contexts. Specifically, CHG methylation is a key factor regulating the expression bias of homoeologs and dominant subgenome expression, as well as stress-responsive gene expression. Additionally, CHH methylation plays a role in peanut seed development by regulating genes associated with fatty acid biosynthesis and lipid metabolism. In conclusion, our study provides a vital theoretical foundation and perspective on the epigenetics underlying cultivated peanut domestication, especially related to the formation of agronomic traits.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"44 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CATIONIC AMINO ACID TRANSPORTER 1 modulates amino acid distribution between stem and leaf in new shoots: a case study of theanine distribution in tea plants (Camellia sinensis) 阳离子氨基酸转运蛋白1调节新芽茎叶间氨基酸分布——以茶树茶氨酸分布为例

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-13 DOI: 10.1093/plphys/kiaf255

Shupei Zhang, Shijia Lin, Ruiping Jin, Yiwen Zhang, Xinxin Zhang, Biying Zhu, Yuanyuan Luo, Byung-Kook Ham, Jieyu Chen, Tianyuan Yang, Xiaochun Wan, Zhaoliang Zhang

{"title":"CATIONIC AMINO ACID TRANSPORTER 1 modulates amino acid distribution between stem and leaf in new shoots: a case study of theanine distribution in tea plants (Camellia sinensis)","authors":"Shupei Zhang, Shijia Lin, Ruiping Jin, Yiwen Zhang, Xinxin Zhang, Biying Zhu, Yuanyuan Luo, Byung-Kook Ham, Jieyu Chen, Tianyuan Yang, Xiaochun Wan, Zhaoliang Zhang","doi":"10.1093/plphys/kiaf255","DOIUrl":"https://doi.org/10.1093/plphys/kiaf255","url":null,"abstract":"Theanine, a tea plant (Camellia sinensis) -specific non-proteinogenic amino acid, is one of the most important components conferring the taste quality and health benefits of tea. It is primarily synthesized in roots of tea plants and transported to new shoots, where it is mainly distributed to the young stem; however, tea is predominantly produced from young leaves. To promote more theanine allocation to young leaves, the molecular mechanism underlying theanine distribution between stems and leaves requires elucidation. In this study, we found the ratios of stem-to-leaf theanine content in the new shoots of 11 tea plant cultivars ranged from 3.8 to 8.8. Analyses on transcriptome and gene expression demonstrated that the expression of CATIONIC AMINO ACID TRANSPORTER1 (CsCAT1), an amino acid transporter-encoding gene, was highly correlated with the ratios of theanine content in the stem and in leaf (r = 0.97, p &lt; 0.0001). Further analyses indicated that CsCAT1 localizes in the plasma membrane and has theanine transport activity. Moreover, CsCAT1 was predominantly expressed in the vascular ray cells in the stem. Finally, we found that repression of CsCAT1 increased theanine content in young leaves and the ratio of leaf-to-stem theanine content. These results indicate that CsCAT1 modulates theanine distribution between stem and leaf and provides a target for increasing theanine content in young leaves of tea plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"10 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brassinosteroid promotes leaf wrinkling in Brassica campestris by regulating a key transcription factor and its downstream genes 油菜素内酯通过调控一个关键转录因子及其下游基因促进油菜叶片起皱

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-12 DOI: 10.1093/plphys/kiaf252

Jingwen Chen, Xiaojie Cai, Yuan Tian, Xuelian Ye, Rui Liu, Yujie Wu, Zihang Ye, Airong Li, Jianli Yang, Xinhua Quan, Xiangtan Yao, Sue Lin, Li Huang

{"title":"Brassinosteroid promotes leaf wrinkling in Brassica campestris by regulating a key transcription factor and its downstream genes","authors":"Jingwen Chen, Xiaojie Cai, Yuan Tian, Xuelian Ye, Rui Liu, Yujie Wu, Zihang Ye, Airong Li, Jianli Yang, Xinhua Quan, Xiangtan Yao, Sue Lin, Li Huang","doi":"10.1093/plphys/kiaf252","DOIUrl":"https://doi.org/10.1093/plphys/kiaf252","url":null,"abstract":"Chinese cabbage (Brassica campestris syn. B.rapa) is an important leafy vegetable featuring a variety of cultivars with diverse leaf morphologies. Among these, leaf wrinkling is a distinctive trait that not only defines cultivar types but also influences key agronomic traits such as light interception, photosynthetic capacity, and consumer preferences related to taste and texture. Despite its importance, the genetic regulation underlying this trait remains unclear. In this study, we compared the transcriptomes of two Chinese cabbage cultivars, ‘Huangya14’ (‘HY14’, with wrinkled leaf) and ‘Suzhouqing’ (‘SZQ’, with flat leaf), at three developmental stages. Clustering analysis suggested that the difference between wrinkled and flat leaves was already apparent 7 days after sowing (DAS). GO and KEGG analysis revealed that a multifaceted network involving adaxial-abaxial axis polarity, epidermal cell division, and brassinosteroid (BR) signaling mediates leaf wrinkling. Notably, the determination of endogenous BR content and exogenous treatment revealed the differentially expressed genes (DEGs) BRASSINAZOLE RESISTANT1 (BcBZR1), PHABULOSA (BcPHB), and CYCLIN D3;1 (BcCYCD3;1), which were further characterized to be sensitive to either endogenous or exogenous BR levels. Overexpression of BcBZR1 and BcPHB contributed to wrinkled leaves with irregular tissue arrangement, increased epidermal cell number, and the elevated expression level of BcCYCD3;1. Meanwhile, silencing the two genes reduced the degree of leaf wrinkling. Interestingly, Y1H, dual-LUC, and ChIP-qPCR assays revealed that BcBZR1 specifically binds to the promoter of BcPHB and BcCYCD3;1 to activate their expression, and BcPHB also promoted the expression of BcCYCD3;1. This study unveils a mechanism whereby BR contributes to leaf wrinkling in Chinese cabbage by regulating the key component BcBZR1 and its downstream genes BcPHB and BcCYCD3;1.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"42 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Illuminating viral replication in living plants: A conditional probe system for tracking replicase dynamics. 照亮活植物中的病毒复制：跟踪复制酶动力学的条件探针系统。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-06-11 DOI: 10.1093/plphys/kiaf250

Thomas Scotti, Sara Shakir

引用次数: 0

N Matters: Insights into nitrogen assimilation from the ectomycorrhizal fungus Laccaria bicolor. 氮问题：外生菌根真菌双色拉卡菌对氮同化的见解。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-06-10 DOI: 10.1093/plphys/kiaf231

Mamoona Khan

引用次数: 0