Plant Physiology最新文献_第2页

The peptide hormone RGF1 modulates PLETHORA2 stability via reactive oxygen species-dependent regulation of a cysteine residue. 肽激素RGF1通过活性氧依赖的半胱氨酸残基调节PLETHORA2的稳定性。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-07-03 DOI: 10.1093/plphys/kiaf244

Yu-Chun Hsiao, Shiau-Yu Shiue, Ming-Ren Yen, Joon-Keat Lai, Masashi Yamada

{"title":"The peptide hormone RGF1 modulates PLETHORA2 stability via reactive oxygen species-dependent regulation of a cysteine residue.","authors":"Yu-Chun Hsiao, Shiau-Yu Shiue, Ming-Ren Yen, Joon-Keat Lai, Masashi Yamada","doi":"10.1093/plphys/kiaf244","DOIUrl":"https://doi.org/10.1093/plphys/kiaf244","url":null,"abstract":"The Root meristem growth factor 1 (RGF1) peptide extends the PLETHORA2 (PLT2) protein gradient by altering the distinct localization of superoxide (O2-) and hydrogen peroxide (H2O2) among the root developmental zones. However, the underlying mechanism through which reactive oxygen species (ROS) regulate PLT2 stability is unclear. Here, we demonstrate that the 212th cysteine of PLT2 is pivotal in modulating PLT2 stability through ROS. The PLT2 protein concentration gradient rapidly decreases in the elongation zone, where H2O2 accumulation initiates. However, substituting the 212th cysteine of PLT2 with serine (PLT2C212S) results in PLT2 being more stable in the elongation zone, more broadly localized by RGF1, and showing robust resistance to H2O2. The sulfenylation of PLT2 was detected following treatment with H2O2 at high concentrations, suggesting that S-sulfenylation of the 212th cysteine controls PLT2 protein stability through local ROS distributions. These findings show that the formation of the PLT2 concentration gradient through ROS depends on a PLT2 sulfenylation mechanism that involves the 212th cysteine.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"198 3","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Running Fermi calculations as a superpower to gauge reality. 运行费米计算，作为衡量现实的超级能力。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-07-03 DOI: 10.1093/plphys/kiae347

Edmar R Oliveira-Filho, Rodrigo Campos-Silva, Andrew D Hanson

引用次数: 0

The transcription factor REM16a promotes flowering time in soybean by activating flowering-related genes. 转录因子REM16a通过激活与开花相关的基因来促进大豆的开花时间。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-07-03 DOI: 10.1093/plphys/kiaf256

Zhikun Wang, Changhuan Du, Qingqing Li, Meng Li, Yuanzhuo Wang, Gege Bao, Yuanyuan Yin, Mingming Yang, Qiang Yang, Pengfei Xu, Shanshan Liu, Bo Song, Shuzhen Zhang

{"title":"The transcription factor REM16a promotes flowering time in soybean by activating flowering-related genes.","authors":"Zhikun Wang, Changhuan Du, Qingqing Li, Meng Li, Yuanzhuo Wang, Gege Bao, Yuanyuan Yin, Mingming Yang, Qiang Yang, Pengfei Xu, Shanshan Liu, Bo Song, Shuzhen Zhang","doi":"10.1093/plphys/kiaf256","DOIUrl":"10.1093/plphys/kiaf256","url":null,"abstract":"The flowering time of soybean [Glycine max (L.) Merr.] is extremely sensitive to photoperiod, which importantly influences its yield potential and restricts the geographical range of soybean cultivars to specific latitudes. Molecular breeding to modulate flowering time and reduce sensitivity to day length is an effective approach to enhance the adaptability and productivity of soybean. Here, we characterized reproductive meristem 16a (GmREM16a), a member of the AP2/B3-like transcription factor family. The GmREM16a protein contains 2 B3 domains, and the expression of its encoding gene is responsive to photoperiod and circadian rhythm. The overexpression of GmREM16a in soybean accelerated flowering by regulating the expression of flowering-related genes. The GmREM16a protein was able to directly bind to the promoters of GmSOC1, GmFT2a, and GmFT5a and upregulate their expression. Yeast two-hybrid screening revealed that GmCSN5 interacts with GmREM16a. GmCSN5 is the fifth subunit of the COP9 signalosome (constitutively photomorphogenic signalosome, CSN) that regulates the activity of CULLIN-RING E3 ubiquitin ligases and regulates protein degradation. Protein degradation assays in vivo and in vitro showed that GmCSN5 promotes the degradation of GmREM16a protein via the ubiquitin-proteasome pathway. Taken together, these findings indicate that the transcription factor GmREM16a promotes flowering by regulating the expression of flowering-related genes. Additionally, GmCSN5 interacts with GmREM16a to regulate its stability in soybean. The GmREM16a-GmSCN5 module may represent a pathway involved in the regulation of flowering time in soybean and is a useful genetic resource for improving the adaptability of soybean through molecular breeding approaches.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How to eat an idea-A roadmap for translation and impact in plant biology. 如何吃一个想法-一个路线图的翻译和影响在植物生物学。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-07-03 DOI: 10.1093/plphys/kiaf178

Johnathan A Napier

引用次数: 0

Conserved language of plant gene regulation. 植物基因调控的保守语言。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-07-03 DOI: 10.1093/plphys/kiaf263

Neeta Lohani

引用次数: 0

Divide and survive: PuZFP1 coordinates dual root developmental pathways for drought adaptation in Populus. 分化与生存：PuZFP1协调杨树干旱适应的双根发育途径。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-07-03 DOI: 10.1093/plphys/kiaf261

Héctor H Torres-Martínez

引用次数: 0

Blue-Light Only: How Horsetails Broke the Rules of Stomatal Control. 只有蓝光：马尾是如何打破气孔控制规则的。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-07-03 DOI: 10.1093/plphys/kiaf298

Ritu Singh, Erin Cullen

引用次数: 0

ETHYLENE INSENSITIVE2-like protein mediates submergence and drought responses in Physcomitrium patens 乙烯insensitive2样蛋白介导了专利状乳胞的浸没和干旱反应

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-07-02 DOI: 10.1093/plphys/kiaf293

Md Masudul Karim, Mousona Islam, Marcos Takeshi Miyabe, Yuko Ikeda, Mohammed Arif Sadik Polash, Kanata Hirota, Hidetoshi Sakayama, Yoichi Sakata, Daisuke Takezawa

{"title":"ETHYLENE INSENSITIVE2-like protein mediates submergence and drought responses in Physcomitrium patens","authors":"Md Masudul Karim, Mousona Islam, Marcos Takeshi Miyabe, Yuko Ikeda, Mohammed Arif Sadik Polash, Kanata Hirota, Hidetoshi Sakayama, Yoichi Sakata, Daisuke Takezawa","doi":"10.1093/plphys/kiaf293","DOIUrl":"https://doi.org/10.1093/plphys/kiaf293","url":null,"abstract":"ETHYLENE INSENSITIVE 2 (EIN2) is an Nramp family transmembrane protein recognized as an essential component of ethylene signaling in angiosperms. However, its functions in other plant systems are not fully understood. Here, we demonstrate that ppein2ab mutants of the moss Physcomitrium patens, in which both EIN2-like genes have been disrupted, do not show a typical ethylene-mediated escape response following submergence. Interestingly, ppein2ab mutants showed reduced sensitivity to abscisic acid (ABA), a phytohormone that mediates drought stress responses. The ppein2ab plants were sensitive to hyperosmosis and freezing stress and exhibited reduced late embryogenesis abundant protein accumulation. Furthermore, ppein2ab mutants showed reduced activation of both SNF1-related protein kinase2 (SnRK2), the central activator of ABA and osmostress signaling, and the B3-RAF kinase ARK/PpCTR1L, a positive regulator of SnRK2. These results indicate that EIN2 is a dual function signaling component mediating both submergence and drought signaling in bryophytes. The diminished ABA responses in ppein2ab were restored by introduction of Arabidopsis EIN2 and the EIN2 orthologs of the Charophyceaen alga Chara braunii, suggesting functional conservation of EIN2 orthologs in Phragmoplastophyta.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"26 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144533073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The susceptibility factor RTP1 negatively regulates Arabidopsis immunity by interacting with the cytochrome P450 protein CYP71B3 易感因子RTP1通过与细胞色素P450蛋白CYP71B3相互作用负向调节拟南芥免疫

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-07-02 DOI: 10.1093/plphys/kiaf284

Yushu Wei, Deqian Zong, Yaling Tang, Lehui Kong, Xianxian Gao, Xiaoxue Wang, Yingqi Zhang, Yang Yang, Xiaoyu Qiang, Weixing Shan

{"title":"The susceptibility factor RTP1 negatively regulates Arabidopsis immunity by interacting with the cytochrome P450 protein CYP71B3","authors":"Yushu Wei, Deqian Zong, Yaling Tang, Lehui Kong, Xianxian Gao, Xiaoxue Wang, Yingqi Zhang, Yang Yang, Xiaoyu Qiang, Weixing Shan","doi":"10.1093/plphys/kiaf284","DOIUrl":"https://doi.org/10.1093/plphys/kiaf284","url":null,"abstract":"Oomycetes, particularly Phytophthora species, cause destructive plant diseases that severely threaten sustainable crop production. Due to the loss of genotype-specific disease resistance, it is important to identify and understand immune factors that mediate plant susceptibility. Loss-of-function of the susceptibility factor Resistance To Phytophthora parasitica 1 (RTP1) leads to broad-spectrum disease resistance in Arabidopsis thaliana(Arabidopsis thaliana (L.) Heynh.). Through RNA-seq analysis, we determined that CYP71B3, encoding an uncharacterized P450 enzyme, is significantly up-regulated in rtp1 mutant plants infected with P. parasitica. Loss-of-function of CYP71B3 led to abolished pathogen-associated molecular pattern (PAMP)-triggered oxidative burst and rendered Arabidopsis more susceptible to diverse pathogens, including the oomycete P. parasitica and bacterial Pseudomonas syringae. Conversely, overexpression of CYP71B3 enhanced plant resistance and PAMP-triggered oxidative burst. CYP71B3 localized in the endoplasmic reticulum and was destabilized by interacting with RTP1 via the I-38 residue, which is essential for its immune function and P450 enzyme activity. The expression of CYP71B3 was regulated by transcription factor bZIP60, which is required for rtp1-mediated resistance to P. parasitica. Our studies indicate that RTP1 mediates plant susceptibility by destabilizing the downstream positive immune factor CYP71B3.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"51 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144533076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Insights into DNA Recognition by HD-Zip Transcription Factors HD-Zip转录因子对DNA识别的分子洞察

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-30 DOI: 10.1093/plphys/kiaf292

Wan Chen, Wei Yan, Kai Jiang, Hongda Huang

{"title":"Molecular Insights into DNA Recognition by HD-Zip Transcription Factors","authors":"Wan Chen, Wei Yan, Kai Jiang, Hongda Huang","doi":"10.1093/plphys/kiaf292","DOIUrl":"https://doi.org/10.1093/plphys/kiaf292","url":null,"abstract":"Homeodomain–leucine zipper (HD-Zip) genes encode a large family of plant-specific transcription factors (TFs) that are integral to plant development, growth, regulation, and responses to environmental and hormonal signals. While the roles and mechanisms of HD-Zip TFs have been extensively studied, the structural basis for their DNA recognition remains unclear. In this study, we analyzed DAP-seq data and identified consensus DNA motifs, 5'-AAT[W]AT-3' and 5'-[N]AAA[N][N]-3', preferentially bound by HD-Zip TFs. Both motifs feature a 5'-AA(T/A)-3' core, which is shared across previously identified HD-Zip target sequences, suggesting a common recognition feature within the HD-Zip family. Focusing on the well-characterized HD-Zip IV TF PROTEIN PRODUCTION FACTOR 2 (PDF2) from Arabidopsis (Arabidopsis thaliana) and its interaction with the L1 box DNA sequence, our structural and biochemical analyses revealed that the PDF2 HD-ZA module forms a dimer to specifically recognize the 5'-AATG-3' core through an asymmetric binding mode. In this mode, only the primary recognition helix of one protomer and the N-arm of the other protomer in the PDF2 HD-ZA dimer are involved in specific DNA interactions. Our study offers insights into the molecular mechanisms of HD-Zip TFs and provides a structural template for engineering applications in agricultural research.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"79 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144516018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0