Plant Physiology最新文献_第2页

Membranous translation platforms in the chloroplast of Chlamydomonas reinhardtii.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-21 DOI: 10.1093/plphys/kiaf111

Yi Sun, Shiva Bakhtiari, Melissa Valente-Paterno, Heng Jiang, William Zerges

{"title":"Membranous translation platforms in the chloroplast of Chlamydomonas reinhardtii.","authors":"Yi Sun, Shiva Bakhtiari, Melissa Valente-Paterno, Heng Jiang, William Zerges","doi":"10.1093/plphys/kiaf111","DOIUrl":"https://doi.org/10.1093/plphys/kiaf111","url":null,"abstract":"A small genome in chloroplasts encodes many of the polypeptide subunits of the photosynthetic electron transport complexes embedded in the membranes of thylakoid vesicles in the chloroplast stroma and synthesized by ribosomes of the bacterial-like genetic system of this semiautonomous organelle. While thylakoid membranes are sites of translation, evidence in the unicellular alga Chlamydomonas reinhardtii supports translation on non-canonical membranes in a discrete translation-zone in the chloroplast. To characterize the membranous platforms for translation and the biogenesis of thylakoid membranes, we profiled membranes during chloroplast development, using the yellow-in-the-dark1 mutant, and carried out proteomic analyses on two membrane types proposed previously to support translation in the chloroplast of C. reinhardtii: \"low-density membrane\" (LDM) and \"chloroplast translation membrane\" (CTM). The results support roles of LDM and CTM in preliminary and ongoing stages of translation, respectively. Proteomics, immunoprecipitation and transmission electron microscopy results support connections of these membranous platforms and a chloroplast envelope domain bound by cytoplasmic ribosomes. Our results contribute to a model of photosynthesis complex biogenesis in a spatiotemporal \"assembly line\" involving LDM and CTM as sequential stages leading to photosynthetic thylakoid membranes.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AtSRGA: A shiny application for retrieving and visualizing stress-responsive genes in Arabidopsis thaliana.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-20 DOI: 10.1093/plphys/kiaf105

Yusuke Fukuda, Kohei Kawaguchi, Atsushi Fukushima

{"title":"AtSRGA: A shiny application for retrieving and visualizing stress-responsive genes in Arabidopsis thaliana.","authors":"Yusuke Fukuda, Kohei Kawaguchi, Atsushi Fukushima","doi":"10.1093/plphys/kiaf105","DOIUrl":"https://doi.org/10.1093/plphys/kiaf105","url":null,"abstract":"Abiotic and biotic stresses pose serious threats to plant productivity. Elucidating the gene regulatory networks involved in plant stress responses is essential for developing future breeding programs and innovative agricultural products. Here, we introduce the AtSRGA (Arabidopsis thaliana Stress Responsive Gene Atlas), a user-friendly application facilitating the retrieval of stress-responsive genes in Arabidopsis (Arabidopsis thaliana). The application was developed using 1,131 microarray and 1,050 RNA sequencing datasets obtained from public databases. These datasets correspond to 11 stress-related conditions, namely abscisic acid, cold, drought, heat, high light, hypoxia, osmotic stress, oxidative stress, salt, wounding, and Pseudomonas syringae pv. tomato DC3000. Using a modified meta-analysis technique known as the vote-counting method, we computed integrated scores to evaluate stress responsiveness for each condition across multiple studies. AtSRGA visualizes gene behavior under 11 stress conditions and offers an interactive, user-friendly interface accessible to all researchers. It presents a comprehensive heatmap of stress-responsive genes, facilitating the comparative analysis of individual stress responses and groups of genes responding to multiple stresses. We validated the expression patterns of several high-scoring genes of unknown function under cold and heat stress using RT-qPCR, thus demonstrating that our application helps select targets to understand stress-responsive gene networks in Arabidopsis. AtSRGA will improve the screening of stress-responsive genes in Arabidopsis, thereby supporting the advancement of plant science toward a sustainable society.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root-knot nematode infection enhances the performance of a specialist root herbivore via plant-mediated interactions.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-20 DOI: 10.1093/plphys/kiaf109

Axel J Touw, Nhu Tran, Andreas Schedl, Jessil A Pajar, Cong Van Doan, Henriette Uthe, Nicole M van Dam

{"title":"Root-knot nematode infection enhances the performance of a specialist root herbivore via plant-mediated interactions.","authors":"Axel J Touw, Nhu Tran, Andreas Schedl, Jessil A Pajar, Cong Van Doan, Henriette Uthe, Nicole M van Dam","doi":"10.1093/plphys/kiaf109","DOIUrl":"https://doi.org/10.1093/plphys/kiaf109","url":null,"abstract":"Herbivores sharing host plants are often temporally and spatially separated, limiting direct interactions between them. Nevertheless, as observed in numerous aboveground study systems, they can reciprocally influence each other via systemically induced plant responses. In contrast, examples of such plant-mediated interactions between belowground herbivores are scarce; however, we postulated that they similarly occur, given the large diversity of root-interacting soil organisms. To test this hypothesis, we analyzed the performance of cabbage root fly (Delia radicum) larvae feeding on the main roots of field mustard (Brassica rapa) plants whose fine roots were infected by the root-knot nematode (Meloidogyne incognita). Simultaneously, we studied the effects of M. incognita on D. radicum-induced defense responses and the accumulation of primary metabolites in the main root. We observed that almost 1.5 times as many D. radicum adults emerged from nematode-infected plants, indicating a facilitation effect of M. incognita infection. Although we observed increases in the accumulation of proteins and two essential amino acids, the strongest effect of nematode infection was visible in the defense response to D. radicum. We observed a 1.5 times higher accumulation of the defense-related phytohormone JA-Ile in response to D. radicum on nematode-infected plants, coinciding with a 75% increase in indole glucosinolate concentrations. Contrastingly, concentrations of aliphatic glucosinolates, secondary metabolites negatively affecting D. radicum, were 10-25% lower in nematode-infected plants. We hypothesize that the attenuated aliphatic glucosinolate concentrations result from antagonistic interactions between biosynthetic pathways of both glucosinolate classes, which was reflected in the expression of key biosynthesis genes. Our results provide explicit evidence of plant-mediated interactions between belowground organisms, likely via systemically induced responses in roots.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermi calculations enable quick downselection of target genes and process optimization in photosynthetic systems. 通过费米计算，可以在光合作用系统中快速向下选择目标基因并优化过程。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-20 DOI: 10.1093/plphys/kiaf103

Ratul Chowdhury, Wheaton Schroeder, Debolina Sarkar, Niaz Bahar Chowdhury, Supantha Dey, Rajib Saha

{"title":"Fermi calculations enable quick downselection of target genes and process optimization in photosynthetic systems.","authors":"Ratul Chowdhury, Wheaton Schroeder, Debolina Sarkar, Niaz Bahar Chowdhury, Supantha Dey, Rajib Saha","doi":"10.1093/plphys/kiaf103","DOIUrl":"https://doi.org/10.1093/plphys/kiaf103","url":null,"abstract":"Understanding how photosynthetic organisms including plants and microbes respond to their environment is crucial for optimizing agricultural practices and ensuring food and energy security, particularly in the context of climactic change and sustainability. This perspective embeds back-of-the-envelope calculations across a photosynthetic organism design and scale up workflow. Starting from the whole system level, we provide a recipe to pinpoint key genetic targets, examine the logistics of detailed computational modeling, explore environmentally driven phenotypes, and feasibility as an industrial biofuel production chassis. While complex computer models or high throughput in vivo studies often dominate scientific inquiry, this perspective highlights the power of simple calculations as a valuable tool for initial exploration and evaluating study feasibility. Fermi calculations are defined as quick, approximate estimations made using back-of-the-envelope calculations and straightforward reasoning to achieve order-of-magnitude accuracy, named after the physicist Enrico Fermi. We show how Fermi calculations, based on fundamental principles and readily available data, can offer a first pass understanding of metabolic shifts in plants and microbes in response to environmental and genetic changes. We also discuss how Fermi checks can be embedded in data-driven advanced computing workflows to enable bio-aware machine learning. Lastly, an understanding of state-of-the-art is necessary to guide study feasibility and identifying key levers to maximize cost to return ratios. Combining biology- and resource- aware Fermi calculations, this proposed approach enables researchers to prioritize resource allocation, identify gaps in predictions and experiments, and develop intuition about how observed responses of plants differ between controlled laboratory environments and industrial conditions.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hijacking Host Metabolism: PstEXLX1 Suppresses Triticum aestivum Defense responses by Targeting a Formate Dehydrogenase 劫持宿主代谢：PstEXLX1 通过靶向甲酸脱氢酶抑制小麦的防御反应

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-03-20 DOI: 10.1093/plphys/kiaf093

Marcella Alves Teixeira, Mamoona Khan

引用次数: 0

The polar-localized borate exporter BOR1 facilitates boron transport in tapetal cells to the developing pollen grains

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-03-19 DOI: 10.1093/plphys/kiaf100

Keita Muro, Arisa Yamasaki, Maki Matsumoto, Yu-Ki Tanaka, Yasumitsu Ogra, Toru Fujiwara, Akira Yoshinari, Junpei Takano

{"title":"The polar-localized borate exporter BOR1 facilitates boron transport in tapetal cells to the developing pollen grains","authors":"Keita Muro, Arisa Yamasaki, Maki Matsumoto, Yu-Ki Tanaka, Yasumitsu Ogra, Toru Fujiwara, Akira Yoshinari, Junpei Takano","doi":"10.1093/plphys/kiaf100","DOIUrl":"https://doi.org/10.1093/plphys/kiaf100","url":null,"abstract":"Boron is an essential micronutrient required for plant cell wall integrity, as it is necessary for crosslinking the pectic polysaccharide rhamnogalacturonan II. Reproductive organs require a greater amount of boron for development and growth compared to vegetative organs. However, the mechanism by which plants distribute boron to specific organs is not fully understood. Under boron-limited conditions, the borate exporter BOR1 plays a central role in transporting boron from the roots to the shoots in Arabidopsis (Arabidopsis thaliana). Here, we found that BOR1 is expressed in the tapetal cells of young anthers in unopened buds, showing polar localization toward the locule where microspores develop. Tapetum-localized BOR1 undergoes endocytosis and is subsequently degraded during anther development. BOR1 degradation occurs independently of the lysine residue at position 590 of BOR1, which is responsible for high boron-induced ubiquitination and degradation. Loss-of-function bor1 mutants exhibit disrupted pollen structure, causing reduced fertility under boron-sufficient conditions in the wild type. These phenotypes were rescued by supplementing with high boron concentrations. Furthermore, inflorescence stem grafting experiments suggested that BOR1-dependent boron transport in the flower is necessary for pollen development and subsequent fertilization under boron-sufficient conditions. Our findings suggest the borate exporter BOR1, together with the previously described boric acid channel NIP7;1, facilitates boron transport in tapetal cells toward the locule, thereby supporting pollen development in young anthers under boron-limited conditions.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"54 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Turn up the red: MADS-RIN-DIVARICATA1 module positively regulates carotenoid biosynthesis in non-climacteric pepper fruits.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-03-19 DOI: 10.1093/plphys/kiaf104

Lara Pereira, Chong Teng

引用次数: 0

The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-03-19 DOI: 10.1093/plphys/kiaf096

Xueying Zhang, Shuting Zhang, Shuangjie Wang, Wentao Ma, Tingkai Zhai, Jie Gao, Chunwang Lai, Zihao Zhang, Yukun Chen, Zhongxiong Lai, Yuling Lin

{"title":"The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan","authors":"Xueying Zhang, Shuting Zhang, Shuangjie Wang, Wentao Ma, Tingkai Zhai, Jie Gao, Chunwang Lai, Zihao Zhang, Yukun Chen, Zhongxiong Lai, Yuling Lin","doi":"10.1093/plphys/kiaf096","DOIUrl":"https://doi.org/10.1093/plphys/kiaf096","url":null,"abstract":"Heat stress can seriously affect plant growth and development. Ethylene response factors (ERFs) play important roles in plant development and physiological responses. Here, we identified DlERF6, an ERF family transcription factor that promotes heat tolerance in Dimocarpus longan. DlERF6 was strongly induced by heat stress and IAA treatment in longan roots. Overexpression of DlERF6 generated abundant, fast-growing hairy roots and enhanced longan heat stress tolerance by promoting IAA biosynthesis and reactive oxygen species (ROS) scavenging. Additional assays indicated that DlERF6 directly binds to the DlGH3.5 promoter and represses its expression. Overexpressing DlGH3.5 reduced hairy root number, root length and heat tolerance, concomitant with a reduction in IAA content and ROS scavenging. Collectively, these results reveal the molecular mechanism through which the DlERF6–DlGH3.5 module regulates root growth and heat stress tolerance, providing a gene network that can be used for the genetic improvement of longan.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"183 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of the cytochrome b6f subunit PetN destabilizes the complex and severely impairs state transitions in Anabaena variabilis 细胞色素 b6f 亚基 PetN 的缺失会破坏复合体的稳定性，并严重影响变色龙藻的状态转换

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-03-12 DOI: 10.1093/plphys/kiaf094

Peijun Wei, Xiying Li, Kun Zhang, Xueang Zhang, Chunxia Dong, Jindong Zhao

{"title":"Loss of the cytochrome b6f subunit PetN destabilizes the complex and severely impairs state transitions in Anabaena variabilis","authors":"Peijun Wei, Xiying Li, Kun Zhang, Xueang Zhang, Chunxia Dong, Jindong Zhao","doi":"10.1093/plphys/kiaf094","DOIUrl":"https://doi.org/10.1093/plphys/kiaf094","url":null,"abstract":"The cytochrome b6f complex (Cyt b6f) plays pivotal roles in both linear and cyclic electron transport of oxygenic photosynthesis in plants and cyanobacteria. The four large subunits of Cyt b6f are responsible for organizing the electron transfer chain within Cyt b6f and have their counterparts in the cytochrome bc1 complex in other bacteria. The four small subunits of Cyt b6f are unique to oxygenic photosynthesis, and their functions remain to be elucidated. Here, we report that Cyt b6f was destabilized by the loss of PetN, one of the small subunits, in a petN mutant (ΔpetN) of Anabaena variabilis ATCC 29413 and that the amount of the large subunits of Cyt b6f decreased to 20-25% of that in the wild-type. The oxygen evolution activity of ΔpetN was approximately 30% of that from the wild-type, and the activity could largely be restored by the addition of N,N,N′, N′-tetramethyl-p-phenylenediamine (TMPD), which functions as an electron carrier and bypasses Cyt b6f. Both linear and cyclic electron transfer of the mutant became partially insensitive to the Cyt b6f inhibitor 2,5-dibromo-3-methyl-6-isopropylbenzoquinone (DBMIB). Although the plastoquinone (PQ) pool was largely reduced in ΔpetN under normal light conditions, the mutant had a substantially higher PSII/PSI ratio than the wild-type. State transitions in ΔpetN were abolished, as revealed by 77K fluorescence spectra and room temperature fluorescence kinetics in the presence of TMPD. Our findings strongly suggest that Cyt b6f is required for state transitions in the cyanobacteria.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"21 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor GmbZIP131 enhances soybean salt tolerance by regulating flavonoid biosynthesis

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-03-12 DOI: 10.1093/plphys/kiaf092

Xiaoli Ni, Yiang Wang, Liqiang Dai, Kedi Jiang, Shuting Zeng, Yixin Huang, Yiting Zhou, Lihui Duan, Cheng Bian, Qi Liu, Hong Sun, Jinbo Shen, Shang Wang, Erxu Pi

{"title":"The transcription factor GmbZIP131 enhances soybean salt tolerance by regulating flavonoid biosynthesis","authors":"Xiaoli Ni, Yiang Wang, Liqiang Dai, Kedi Jiang, Shuting Zeng, Yixin Huang, Yiting Zhou, Lihui Duan, Cheng Bian, Qi Liu, Hong Sun, Jinbo Shen, Shang Wang, Erxu Pi","doi":"10.1093/plphys/kiaf092","DOIUrl":"https://doi.org/10.1093/plphys/kiaf092","url":null,"abstract":"Detoxifying reactive oxygen species (ROS) that accumulate under saline conditions is crucial for plant salt tolerance. The Salt Overly Sensitive (SOS) pathway functions upstream, while flavonoids act downstream, in ROS scavenging under salt stress. However, the potential crosstalk between the SOS pathway and flavonoids in regulating salt stress responses and the associated mechanisms remain largely unexplored. To assess this possible connection, we investigated the role of the soybean (Glycine max) transcription factor GmbZIP131 in enhancing salt tolerance by modulating ROS homeostasis through flavonoid biosynthesis. GmSOS2 like (GmSOS2L), a key component of the SOS pathway, phosphorylates and activates GmbZIP131, thus promoting GmICHG (isoflavone conjugate-specific beta-glucosidase) expression. Metabolic profiling of transgenic soybean lines revealed that GmbZIP131 upregulates the levels of lupiwighteone and its 7-glucoside precursor, likely processed by GmICHG. Furthermore, overexpression of GmSOS2L, GmbZIP131, or GmICHG enhances the accumulation of lupiwighteone and its 7-glucoside precursor, as well as soybean salt stress tolerance. Our findings reveal a GmSOS2L–GmbZIP131–GmICHG signaling cascade that enhances soybean salt tolerance through flavonoid accumulation. This research uncovers a mechanism linking the SOS pathway to flavonoid metabolism, offering insights for improving soybean stress tolerance and advancing the molecular breeding of salt-tolerant varieties.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"22 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0