Plant Physiology最新文献_第2页

The transcription factor WRKY41–FLAVONOID 3′-HYDROXYLASE module fine-tunes flavonoid metabolism and cold tolerance in potato

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf070

Huihui Bao, Li Yuan, Yongchao Luo, Jinxiu Zhang, Xi Liu, Qiuju Wu, Xiyao Wang, Jitao Liu, Guangtao Zhu

{"title":"The transcription factor WRKY41–FLAVONOID 3′-HYDROXYLASE module fine-tunes flavonoid metabolism and cold tolerance in potato","authors":"Huihui Bao, Li Yuan, Yongchao Luo, Jinxiu Zhang, Xi Liu, Qiuju Wu, Xiyao Wang, Jitao Liu, Guangtao Zhu","doi":"10.1093/plphys/kiaf070","DOIUrl":"https://doi.org/10.1093/plphys/kiaf070","url":null,"abstract":"Cold stress adversely affects crop growth and productivity. Resolving the genetic basis of freezing tolerance is important for crop improvement. Wild potato (Solanum commersonii) exhibits excellent freezing tolerance. However, the genetic factors underlying its freezing tolerance remain poorly understood. Here, we identified flavonoid 3’-hydroxylase (F3’H), a key gene in the flavonoid biosynthesis pathway, as highly expressed in S. commersonii compared to cultivated potato (S. tuberosum L.). Loss of ScF3’H function impaired freezing tolerance in S. commersonii, while ScF3’H overexpression in cultivated potato enhanced its freezing tolerance. Metabolic analysis revealed that F3’H generates more downstream products by adding hydroxyl (-OH) groups to the flavonoid ring structures. These flavonoids enhance reactive oxygen species scavenging, thereby contributing to freezing tolerance. Furthermore, the W-box element in the F3’H promoter plays a critical role in cold responses. Cold-induced transcription factor ScWRKY41 directly binds to the ScF3’H promoter region and recruits histone acetyltransferase 1 (ScHAC1), which enhances histone acetylation at the F3’H locus and activates its transcription. Overall, we identified the cold-responsive WRKY41–F3’H module that enhances freezing tolerance by augmenting the antioxidant capacity of flavonoids. This study reveals a valuable natural gene module for breeding enhanced freezing tolerance in potato and other crops.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"65 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial DNA and the largest nuclear-mitochondrial DNA in Arabidopsis can be separated by their methylation levels.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf069

Yuyang Zhong, Miki Okuno, Nobuhiro Tsutsumi, Shin-Ichi Arimura

{"title":"Mitochondrial DNA and the largest nuclear-mitochondrial DNA in Arabidopsis can be separated by their methylation levels.","authors":"Yuyang Zhong, Miki Okuno, Nobuhiro Tsutsumi, Shin-Ichi Arimura","doi":"10.1093/plphys/kiaf069","DOIUrl":"https://doi.org/10.1093/plphys/kiaf069","url":null,"abstract":"Methylation of cytosines in plant mitochondrial DNA (mtDNA) has been a controversial issue. Results supporting mtDNA methylation may have been subject to contamination due to the presence of nuclear sequences originating from the mitochondrial genome called nuclear mitochondrial DNA insertions (NUMT). In Arabidopsis (Arabidopsis thaliana) Columbia 0 (Col-0), the largest NUMT, located on chromosome 2, is nearly twice the size of the entire mitochondrial genome and exhibits a sequence almost identical to the mitochondrial genome, albeit with shuffling and repeats. In the presence of such high similarity, it is challenging to eliminate interference when determining mtDNA methylation levels. Here, we applied a methyl-CpG binding domain (MBD) protein-based affinity assay to separate total DNA, applied next-generation sequencing to the pre- and post-separation DNA samples, and examined the SNP sites between NUMT and mtDNA. The results revealed successful separation of methylated and non-methylated DNA within the total DNA, with simultaneous separation achieved between NUMT DNA and mtDNA. These results suggest that our method can achieve separation based on the differential methylation levels of the whole lengths of NUMT and mitochondrial DNAs. The bisulfite sequencing results for the post-separation DNA samples suggest that mtDNA exhibits not only a lack of CpG methylation but also an absence of CHH and CHG methylation. In contrast, the NUMT shows high levels of methylation across all three contexts, at least in the Columbia 0 accession.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimycin A induces light hypersensitivity of photosystem II in the presence of quinone QB-site binding herbicides

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf082

Ko Imaizumi, Daisuke Takagi, Kentaro Ifuku

{"title":"Antimycin A induces light hypersensitivity of photosystem II in the presence of quinone QB-site binding herbicides","authors":"Ko Imaizumi, Daisuke Takagi, Kentaro Ifuku","doi":"10.1093/plphys/kiaf082","DOIUrl":"https://doi.org/10.1093/plphys/kiaf082","url":null,"abstract":"Photosynthetic electron transport consists of linear electron flow and two cyclic electron flow (CEF) pathways around photosystem I (PSI) (CEF-PSI). PROTON GRADIENT REGULATION 5 (PGR5)-dependent CEF-PSI is thought to be the major CEF-PSI pathway and an important regulator of photosynthetic electron transfer. Antimycin A (AA) is commonly recognized as an inhibitor of PGR5-dependent CEF-PSI in photosynthesis. Although previous findings imply that AA may also affect photosystem II (PSII), which does not participate in CEF-PSI, these “secondary effects” tend to be neglected, and AA is often used for inhibition of PGR5-dependent CEF-PSI as if it were a specific inhibitor. Here, we investigated the direct effects of AA on PSII using isolated spinach (Spinacia oleracea) PSII membranes, and thylakoid membranes isolated from spinach, Arabidopsis thaliana (wild-type Columbia-0 and PGR5-deficient mutant pgr5hope1), and Chlamydomonas reinhardtii. Measurements of quinone QA− reoxidation kinetics showed that AA directly affects the acceptor side of PSII and inhibits electron transport within PSII. Furthermore, repetitive Fv/Fm measurements revealed that, in the presence of quinone QB-site binding inhibitors, AA treatment results in severe photodamage even from a single-turnover flash. The direct effects of AA on PSII are non-negligible and caution is required when using AA as an inhibitor of PGR5-dependent CEF-PSI. Meanwhile, we found that the commercially available compound AA3, which is a component of the AA complex, inhibits PGR5-dependent CEF-PSI without having notable effects on PSII. Thus, we propose that AA3 should be used instead of AA for physiological studies of the PGR5-dependent CEF-PSI.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"65 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrate reductase-dependent nitric oxide production mediates nitrate-conferred salt tolerance in rice seedlings

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf080

Zhenning Teng, Qin Zheng, Yaqiong Peng, Yi Li, Shuan Meng, Bohan Liu, Yan Peng, Meijuan Duan, Dingyang Yuan, Jianhua Zhang, Nenghui Ye

{"title":"Nitrate reductase-dependent nitric oxide production mediates nitrate-conferred salt tolerance in rice seedlings","authors":"Zhenning Teng, Qin Zheng, Yaqiong Peng, Yi Li, Shuan Meng, Bohan Liu, Yan Peng, Meijuan Duan, Dingyang Yuan, Jianhua Zhang, Nenghui Ye","doi":"10.1093/plphys/kiaf080","DOIUrl":"https://doi.org/10.1093/plphys/kiaf080","url":null,"abstract":"Soil salinity is a destructive environmental factor that inhibits plant growth and crop yield. Applying nitrogen fertilizer is a practical method to enhance salt tolerance. However, the underlying mechanisms remain largely unknown. Here, we demonstrated that NO3−-enhanced salt tolerance in rice (Oryza sativa L.) seedlings is mediated by nitrate reductase (NR)-dependent nitric oxide (NO) production. Seedlings grown in nitrate condition (N) exhibited much greater salt tolerance compared to those grown in ammonium nitrate (AN) and ammonium (A) conditions, a pattern also observed in the MADS-box transcription factor 27 (mads27) mutant. NR activity was highly induced by NO3− under both normal and salt stress conditions. Only the double mutant nr1/2 and the triple mutant nr1/2/3 displayed a dramatic reduction in salt tolerance. Application of tungstate suppressed salt tolerance of wild-type seedlings but not the triple mutants. Furthermore, both NO3−-enhanced salt tolerance and salt-induced NO production were totally blocked in triple mutants. However, treatment with exogenous sodium nitroprusside (an NO donor) significantly enhanced salt tolerance in both NIP and the triple mutants. Antioxidant enzyme activities in shoots were significantly inhibited in the triple mutants when compared with NIP. Furthermore, expression of OsAKT1 was specifically induced by NO3− but was inhibited in the roots of triple mutants, resulting in a lower potassium/sodium ratio in NR triple mutants. Our results revealed that NO3−-conferred salt tolerance is mediated by NR-dependent NO production in rice seedlings.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"48 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CYTOKININ DEHYDROGENASE suppression increases intrinsic water use efficiency and photosynthesis in cotton under drought

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf081

Wei Hu, Dimitra A Loka, Yuanyu Luo, Huilian Yu, Shanshan Wang, Zhiguo Zhou

{"title":"CYTOKININ DEHYDROGENASE suppression increases intrinsic water use efficiency and photosynthesis in cotton under drought","authors":"Wei Hu, Dimitra A Loka, Yuanyu Luo, Huilian Yu, Shanshan Wang, Zhiguo Zhou","doi":"10.1093/plphys/kiaf081","DOIUrl":"https://doi.org/10.1093/plphys/kiaf081","url":null,"abstract":"Drought reduces endogenous cytokinin (CK) content and disturbs plant water balance and photosynthesis. However, the effect of higher endogenous CK levels (achieved by suppressing cytokinin dehydrogenase (CKX) genes) on plant water status and photosynthesis under drought stress is unknown. Here, pot experiments were conducted with wild-type (WT) cotton (Gossypium hirsutum) and two GhCKX suppression lines (CR-3 and CR-13) to explore the effect of higher endogenous CK levels on leaf water utilization and photosynthesis under drought stress. The GhCKX suppression lines had higher leaf net photosynthetic rate (AN) and intrinsic water use efficiency (iWUE) than WT under drought. This increase was attributed to the decoupling of stomatal conductance (gs) and mesophyll conductance (gm) in the suppression lines in response to drought. GhCKX suppression increased gm but maintained gs relative to WT under drought, and the increased gm was associated with altered anatomical traits, including decreased cell wall thickness (Tcw) and increased surface area of chloroplast-facing intercellular airspaces per unit leaf area (Sc/S), as well as altered cell wall composition, especially decreased cellulose levels. This study provides evidence that increased endogenous CK levels can simultaneously enhance AN and iWUE in cotton under drought conditions and establishes a potential mechanism for this effect. These findings provide a potential strategy for breeding drought-tolerant crops or exploring alternative methods to promote crop drought tolerance.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"47 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The wheat stripe rust effector PstEXLX1 inhibits formate dehydrogenase activity to suppress immunity in wheat

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf083

Jingwei Lu, Kexin Sun, Wenxin Yang, Ying Mou, Ruijie Zhang, Ralf T Voegele, Zhensheng Kang, Jia Guo, Jun Guo

{"title":"The wheat stripe rust effector PstEXLX1 inhibits formate dehydrogenase activity to suppress immunity in wheat","authors":"Jingwei Lu, Kexin Sun, Wenxin Yang, Ying Mou, Ruijie Zhang, Ralf T Voegele, Zhensheng Kang, Jia Guo, Jun Guo","doi":"10.1093/plphys/kiaf083","DOIUrl":"https://doi.org/10.1093/plphys/kiaf083","url":null,"abstract":"Effectors are the most critical weapons that Puccinia striiformis f. sp. tritici (Pst) employs to engage with wheat (Triticum aestivum L.). Discovering important effectors is essential for deciphering the pathogenic mechanisms of Pst. In this study, we identified the expansin-like protein 1from Pst (PstEXLX1), which suppresses cell death in Nicotiana benthamiana. In wheat, knockdown of PstEXLX1 diminished Pst development, whereas PstEXLX1 overexpression enhanced Pst virulence by inhibiting PAMP-triggered immunity (PTI), indicating its importance in pathogenesis. Further investigation revealed that PstEXLX1 stabilizes itself through self-association mediated by its expansin-like domain, which also determines its association with the wheat formate dehydrogenase TaFDH1. Wheat lines overexpressing TaFDH1 exhibited increased resistance to Pst, which was associated with elevated TaFDH1 catalytic activity and induced defense responses. In addition, TaFDH1 activity was strongly inhibited in the presence of PstEXLX1 but became more robust in PstEXLX1-silenced plants, suggesting that PstEXLX1 suppresses TaFDH1 activity. Collectively, our results uncover a strategy employed by Pst to facilitate infection, wherein PstEXLX1 suppresses TaFDH1 activity to repress host immune responses.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"81 1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MADS6, JAGGED, and YABBY proteins synergistically determine floral organ development in rice

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-02-20 DOI: 10.1093/plphys/kiaf076

Zhengzheng Cai, Jieqiong Li, Yuanyuan Su, Lili Zheng, Jianwei Zhang, Miaomiao Zhu, Bingwen Qiu, Lan Kong, Yanfang Ye, Yongbiao Xue, Weiren Wu, Yuanlin Duan

{"title":"The MADS6, JAGGED, and YABBY proteins synergistically determine floral organ development in rice","authors":"Zhengzheng Cai, Jieqiong Li, Yuanyuan Su, Lili Zheng, Jianwei Zhang, Miaomiao Zhu, Bingwen Qiu, Lan Kong, Yanfang Ye, Yongbiao Xue, Weiren Wu, Yuanlin Duan","doi":"10.1093/plphys/kiaf076","DOIUrl":"https://doi.org/10.1093/plphys/kiaf076","url":null,"abstract":"MADS6, JAGGED (JAG), and DROOPING LEAF (DL) are key regulators of floral organ patterns in rice (Oryza sativa); however, how they work together in specifying floral organs remains to be determined. Here, we extensively analyzed the coordination mechanism. Genetic interactions showed that all double/triple mutant combinations of mads6-5 with jag and/or dl-sup7 generated an inflorescence from the spikelet center and lemma-like organs (LLOs) at the periphery, indicating that these genes synergistically promote floral organ specification, inhibit inflorescence initiation, and terminate the floral meristem (FM). Particularly, a fully developed mads6-5 jag spikelet appeared as a large bouquet composed of numerous multi-floral complexes (MFC), while the triple mutant was generally similar to mads6-5 jag, except for a longer pedicel and fewer MFCs. Expression analysis revealed that JAG directly inhibits the transcription of MADS6 in stamens but not in pistils, as JAG and DL co-express in pistils and form a JAG-DL complex, indicating that JAG and DL may coordinate the transcription of MADS6 in sexual organs. Protein interactions revealed that MADS6 and JAG bind to five spikelet-related YABBY proteins (including DL), forming ten heterodimers, suggesting that they may promote floral differentiation through various pathways. However, MADS6 and JAG neither bound together nor formed a heterotrimer with any of the five YABBY proteins. These findings revealed specific synergistic patterns between MADS6, JAGGED, and YABBY proteins, which may contribute to the unique characteristics of rice spikelets and provide insights into the diversity regulation mechanisms of floral specification in plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"52 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caffeic acid O-methyltransferase-dependent flavonoid defenses promote sorghum resistance to fall armyworm infestation.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-02-19 DOI: 10.1093/plphys/kiaf071

Pritha Kundu, Sanket Shinde, Sajjan Grover, Scott E Sattler, Joe Louis

{"title":"Caffeic acid O-methyltransferase-dependent flavonoid defenses promote sorghum resistance to fall armyworm infestation.","authors":"Pritha Kundu, Sanket Shinde, Sajjan Grover, Scott E Sattler, Joe Louis","doi":"10.1093/plphys/kiaf071","DOIUrl":"https://doi.org/10.1093/plphys/kiaf071","url":null,"abstract":"Sorghum (Sorghum bicolor), one of the world's most important monocot crops, suffers severe yield losses due to attack by a polyphagous insect pest, fall armyworm (FAW; Spodoptera frugiperda). Here, we show that the Brown midrib 12 (Bmr12) gene, which encodes the caffeic acid O-methyltransferase (COMT) enzyme, promotes sorghum defense against FAW. Loss of Bmr12 function resulted in increased susceptibility, but enhanced resistance to FAW was observed in Bmr12-overexpression (OE) plants compared with wild-type (RTx430) plants. Although COMT is associated with modulating lignin levels, FAW infestation resulted in comparable lignin levels between bmr12 and Bmr12-OE sorghum plants. On the contrary, evidence presented here indicates that FAW feeding induced the accumulation of flavonoids, which was previously shown to have a negative impact on FAW growth and survival in Bmr12-OE plants compared with bmr12 and RTx430 plants. Furthermore, a combination of phytohormone profiling and transcriptomic analysis uncovered that COMT-mediated resistance to FAW depends on jasmonic acid (JA) and oxidative stress-associated pathways. Exogenous application of FAW oral secretions stimulated flavonoid accumulation in Bmr12-OE plants compared with bmr12 and RTx430 plants, indicating that COMT has an essential function in perceiving FAW oral cues. Taken together, the critical role of COMT in sorghum defense against FAW hinges upon the interplay between JA and its derivatives and hydrogen peroxide, which potentially helps to mount a robust flavonoid-based host defense upon caterpillar attack.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GT1 and ZmHB13/VRL1 regulate flower sexual differentiation by modulating jasmonate biosynthesis and signaling in maize.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-02-19 DOI: 10.1093/plphys/kiaf075

Yateng Yuan, Xiaotong Ou, Minhao Yao, Pinle Wang, Chunlian Li, Guisen Zhang, Mengqi He, Heying Li, Xin Xu, Zhuojun Zhong, Yun Lu, Xiaojun Lai, Chen Zou, Jie Shen, Tianyu Zhang, Defan Liu, Yaoyao Li, Haiyang Wang, Dexin Kong, Qing Liu

{"title":"GT1 and ZmHB13/VRL1 regulate flower sexual differentiation by modulating jasmonate biosynthesis and signaling in maize.","authors":"Yateng Yuan, Xiaotong Ou, Minhao Yao, Pinle Wang, Chunlian Li, Guisen Zhang, Mengqi He, Heying Li, Xin Xu, Zhuojun Zhong, Yun Lu, Xiaojun Lai, Chen Zou, Jie Shen, Tianyu Zhang, Defan Liu, Yaoyao Li, Haiyang Wang, Dexin Kong, Qing Liu","doi":"10.1093/plphys/kiaf075","DOIUrl":"https://doi.org/10.1093/plphys/kiaf075","url":null,"abstract":"As a typical monoecious plant, maize (Zea mays L.) produces unisexual male and female flowers comprising tassels and ears, respectively. However, the molecular mechanisms underlying unisexual flower development remain largely unclear. Here, we identify a pair of homeodomain-leucine zipper (HD-ZIP) transcription factors, Grassy tiller1 (GT1) and ZmHB13 (also termed VRS1-like1, VRL1), which act synergistically to control carpel development in maize. Mutations in GT1, but not ZmHB13/VRL1, cause defects in carpel abortion in the tassels, resulting in feminized tassels. The gt1 Zmhb13 double mutants show a notably more severe phenotype and a defect in the abortion of the lower floret in the ear spikelet, resulting in extra fertile flowers and seed setting. We demonstrate that GT1 and ZmHB13/VRL1 bind directly to the promoters of two jasmonate (JA) biosynthesis genes, ZmLOX3 and ZmOPR7, and upregulate their expression in the developing flowers. The application of methyl jasmonate (MeJA) rescues the feminized tassel phenotype of the gt1 Zmhb13 double mutants. Additionally, GT1 and ZmHB13/VRL1 interact with the key JA-pathway repressors ZmJAZ4 and ZmJAZ26, thus releasing the key JA-pathway activators ZmMYC2a and ZmMYC2b to activate the downstream JA signaling processes. Our findings indicate that GT1 and ZmHB13/VRL1 function synergistically to regulate maize carpel development through modulating JA biosynthesis and signaling.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Bacterial-Plant Auxin Alliance Enhances Phosphorus Acquisition.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-02-18 DOI: 10.1093/plphys/kiaf061

Haiyan Yu, Jiaqi Sun

引用次数: 0