Plant Physiology最新文献_第2页

Enhancing photosynthesis under salt stress via directed evolution in cyanobacteria 通过蓝藻定向进化增强盐胁迫下的光合作用

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-21 DOI: 10.1093/plphys/kiaf209

Zhenxiong Jiang, Khondokar Nowshin Islam, Malory Wolfe, Michael O’Connell, Dykia Williams, Ashley Florance, David J Vinyard, Xiaohui Zhang, Maxwell Brenner, Andor J Kiss, Xianhua Liu, Xin Wang

{"title":"Enhancing photosynthesis under salt stress via directed evolution in cyanobacteria","authors":"Zhenxiong Jiang, Khondokar Nowshin Islam, Malory Wolfe, Michael O’Connell, Dykia Williams, Ashley Florance, David J Vinyard, Xiaohui Zhang, Maxwell Brenner, Andor J Kiss, Xianhua Liu, Xin Wang","doi":"10.1093/plphys/kiaf209","DOIUrl":"https://doi.org/10.1093/plphys/kiaf209","url":null,"abstract":"A key aspect of enhancing photosynthesis is improving the kinetics of photochemical quenching recovery following environmental perturbation or stress. Salt stress exacerbates high light stress in cyanobacteria and leads to severe yield losses in crop plants. Genetic traits that confer salt tolerance without compromising photosynthetic performance are essential for improving photosynthesis under these conditions. Here, we applied accelerated evolution in Synechococcus elongatus PCC 7942 by conditionally suppressing its methyl-directed mismatch repair system to obtain beneficial genetic traits for enhanced photosynthesis under salt stress. We screened over 10,000 mutants and isolated eight strains with increased biomass or sucrose productivity under salt stress. Genome sequencing revealed an average of 8–20 single nucleotide polymorphisms (SNPs) or indels per genome. Notably, mutations in the photosystem II (PSII) reaction center D1-encoding gene, resulting in the amino acid changes L353F, I358N, and H359N at the carboxyl terminus of the precursor-D1 (pD1) protein, improved photosynthesis under salt and combined salt and light stress by potentially accelerating D1 maturation during PSII repair. Phylogenetic analysis of pD1 across cyanobacteria and red algae highlights the broad relevance of these adaptive genetic traits, underscoring the importance of leveraging evolutionary insights to improve photosynthesis under stress or fluctuating environments.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"37 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144114077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaf oil under stress: Divergent lipid synthesis and stabilization strategies in wild-type and engineered tobacco. 胁迫下的叶油：野生型和工程型烟草不同的脂质合成和稳定策略。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-15 DOI: 10.1093/plphys/kiaf200

Sara Shakir

引用次数: 0

Functional characterization of the N assimilation pathways in the mycelium of Laccaria bicolor and the ectomycorrhizal symbiosis 双色拉毛菌菌丝体与外生菌根共生N同化途径的功能表征

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-15 DOI: 10.1093/plphys/kiaf194

Xinyin Zhang, Jianan Ma, Pan Zhang, Wensheng Shi, Rong Zou, Annegret Kohler, Yingli Yang, Francis M Martin, Feng Zhang

{"title":"Functional characterization of the N assimilation pathways in the mycelium of Laccaria bicolor and the ectomycorrhizal symbiosis","authors":"Xinyin Zhang, Jianan Ma, Pan Zhang, Wensheng Shi, Rong Zou, Annegret Kohler, Yingli Yang, Francis M Martin, Feng Zhang","doi":"10.1093/plphys/kiaf194","DOIUrl":"https://doi.org/10.1093/plphys/kiaf194","url":null,"abstract":"Ectomycorrhizal (ECM) fungi contribute to N, Pi, and water uptake in trees while obtaining carbohydrates from their host plants. However, the molecular mechanisms underlying N assimilation during ECM symbiosis remain unclear. In this study, we used RNA interference (RNAi) to silence the expression of genes encoding glutamine synthetase (GS) and NADP-glutamate dehydrogenase (GDH), which are key enzymes involved in N assimilation in the ectomycorrhizal basidiomycete, Laccaria bicolor. LbGS and LbGDH RNAi strains exhibited significantly reduced mycelial growth when cultivated with various inorganic N sources. Compared to the wild-type mycelium, the RNAi strains demonstrated a reduced formation rate of ECM rootlets, indicating the essential role of these two enzymes in the establishment of symbiosis. Transcriptomic analysis revealed that silencing of LbGS and LbGDH also altered the expression of other genes involved in N metabolism in ECM rootlets. 15N and 13C tracer experiments demonstrated that LbGS silencing affects carbon exchange in ECM roots. Our findings have established that both GS and NADP-GDH pathways play crucial roles in N assimilation in free-living mycelia and ECM roots, although the GS/GOGAT pathway appears to be predominant.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"19 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143979589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-Hydroxypipecolic acid and salicylic acid play key roles in autoimmunity induced by loss of the callose synthase PMR4 n -羟基果酸和水杨酸在胼胝质合成酶PMR4缺失引起的自身免疫中起关键作用

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-15 DOI: 10.1093/plphys/kiaf163

Baofang Fan, Zizhang Li, Amber S Jannasch, Shunyuan Xiao, Zhixiang Chen

{"title":"N-Hydroxypipecolic acid and salicylic acid play key roles in autoimmunity induced by loss of the callose synthase PMR4","authors":"Baofang Fan, Zizhang Li, Amber S Jannasch, Shunyuan Xiao, Zhixiang Chen","doi":"10.1093/plphys/kiaf163","DOIUrl":"https://doi.org/10.1093/plphys/kiaf163","url":null,"abstract":"In Arabidopsis thaliana, the POWDERY MILDEW RESISTANT4 (PMR4)/GLUCAN SYNTHASE LIKE5 (GSL5) callose synthase is required for pathogen-induced callose deposition in cell wall defense. Paradoxically, pmr4/gsl5 mutants exhibit strong resistance to both powdery and downy mildew. The powdery mildew resistance of pmr4/gsl5 has been attributed to up-regulated salicylic acid (SA) signaling based on its dependence on PHYTOALEXIN DEFICIENT4 (PAD4), which controls SA accumulation, and its abolishment by bacterial NahG salicylate hydroxylase. Our study revealed that disruption of PMR4/GSL5 also leads to early senescence. Suppressor analysis uncovered that PAD4 and N-hydroxypipecolic acid (NHP) biosynthetic genes ABERRANT GROWTH AND DEATH2-LIKE DEFENSE RESPONSE PROTEIN1 (ALD1) and FLAVIN-DEPENDENT MONOXYGENASE1 (FMO1) are required for early senescence of pmr4/gsl5 mutants. The critical role of NHP in the early senescence of pmr4/gsl5 was supported by greatly increased accumulation of pipecolic acid in pmr4/gsl5 mutants. In contrast, disruption of the SA biosynthetic gene ISOCHORISMATE SYNTHASE1/SA-INDUCTION DIFFICIENT 2 (ICS1/SID2), which greatly reduces SA accumulation, had little effect on impaired growth of pmr4/gsl5. Furthermore, while disruption of PAD4 completely abolished the powdery mildew resistance in pmr4/gsl5, mutations in ICS1/SID2, ALD1, or FMO1 had only a minor effect on the resistance of the mutant plants. However, disruption of both ICS1/SID2 and FMO1 abolished the enhanced immunity of the callose synthase mutants against the fungal pathogen. Therefore, while NHP plays a crucial role in the early senescence of pmr4/gsl5 mutants, both SA and NHP have important roles in the strong powdery mildew resistance induced by the loss of the callose synthase.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143979590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building resistance: stomatal and mesophyll conductance as variables limiting photosynthetic induction. 建设阻力：气孔和叶肉导度作为限制光合诱导的变量。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-15 DOI: 10.1093/plphys/kiaf199

Pablo Ignacio Calzadilla

引用次数: 0

PuUBL5-mediated ZINC FINGER PROTEIN 1 stability is critical for root development under drought stress in Populus ussuriensis. puubl5介导的锌指蛋白1的稳定性对干旱胁迫下乌苏杨根系发育至关重要。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-14 DOI: 10.1093/plphys/kiaf181

Haoqin Zhao,Yanrui Fu,Wanqiu Lv,Xin Zhang,Jingjing Li,Da Yang,Lin Shi,Hanzeng Wang,Wanxin Li,Haijiao Huang,Shicheng Zhao,Chenghao Li,Jingli Yang

{"title":"PuUBL5-mediated ZINC FINGER PROTEIN 1 stability is critical for root development under drought stress in Populus ussuriensis.","authors":"Haoqin Zhao,Yanrui Fu,Wanqiu Lv,Xin Zhang,Jingjing Li,Da Yang,Lin Shi,Hanzeng Wang,Wanxin Li,Haijiao Huang,Shicheng Zhao,Chenghao Li,Jingli Yang","doi":"10.1093/plphys/kiaf181","DOIUrl":"https://doi.org/10.1093/plphys/kiaf181","url":null,"abstract":"C2H2-type zinc finger protein (ZFP) transcription factors influence root growth and development. However, their potential roles in inhibiting adventitious root (AR) and lateral root (LR) formation in trees remain unclear. Here, we report that the ABA-responsive C2H2-type zinc finger protein transcription factor (PuZFP1) regulates Populus ussuriensis root development to enhance drought tolerance. PuZFP1 negatively regulates LR development by binding to the PuWRKY46 promoter and inhibiting its expression. At the same time, PuZFP1 promotes AR elongation by repressing Clade E Growth-Regulating (EGR) Type 2C protein phosphatases (PuEGR1). In PuZFP1-overexpressing lines, a higher ABA/IAA ratio in the differentiation zone (DZ) drives PuWRKY46-mediated LR inhibition. Conversely, a lower ABA/IAA ratio is associated with AR elongation and the expression of the downstream target gene PuEGR1 in the elongation zone (EZ). Notably, PuZFP1 physically interacts with Ubiquitin-like protein 5 (PuUBL5) and undergoes 26S proteasome-mediated degradation. Taken together, our findings shed light on the role of the PuUBL5-PuZFP1 module in mediating the crosstalk between LR emergence and AR elongation via ABA/auxin signaling in drought-stressed P. ussuriensis, and provide insights into the regulatory network underlying PuZFP1-mediated root growth in poplar.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methionine as a key player in salt stress adaptation in plants. 蛋氨酸在植物适应盐胁迫中的重要作用。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-14 DOI: 10.1093/plphys/kiaf169

Xuelian Li,Ning Zhang

引用次数: 0

Tomato CONSTANS-Like1 promotes anthocyanin biosynthesis under short day and suboptimal low temperature. 番茄CONSTANS-Like1在短日照和次优低温条件下促进花青素的生物合成。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-13 DOI: 10.1093/plphys/kiaf190

Sai Liu,Shanwu Lyu,Yi Zhang,Siqi Liu,Shulin Deng

{"title":"Tomato CONSTANS-Like1 promotes anthocyanin biosynthesis under short day and suboptimal low temperature.","authors":"Sai Liu,Shanwu Lyu,Yi Zhang,Siqi Liu,Shulin Deng","doi":"10.1093/plphys/kiaf190","DOIUrl":"https://doi.org/10.1093/plphys/kiaf190","url":null,"abstract":"Plant growth and development are precisely controlled by light and temperature during their life span. However, the mechanism by which photoperiod and seasonal changes influence the physiological response of day-neutral plants, such as tomato (Solanum lycopersicum), remains unclear. Here, we found that the tomato CONSTANS (CO) close homolog, CONSTANS-Like1 (SlCOL1), does not affect the flowering of tomato under either long-day (LD) or short-day (SD) conditions. However, CRISPR/Cas9-mediated editing of SlCOL1 showed a much lower anthocyanin accumulation in mutant than in wild-type plants, especially under SD at suboptimal low-temperature conditions. SlCOL1 directly activated the Hoffman's Anthocyanin 1 (SlAN1) promoter and interacted with SlAN1 to promote anthocyanin biosynthesis under SD. The cold-induced up-regulation of SlCOL1 further promoted anthocyanin accumulation and enhanced Reactive oxygen species (ROS) scavenging under SD at low-temperature conditions. These results reveal that the SlCOL1-SlAN1 module collaboratively regulates anthocyanin accumulation under SD and cold conditions, which could help tomato counteract the cold autumn/winter season in nature.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"164 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The N6-methyladenosine reader ECT1 regulates seed germination via gibberellic acid- and phytochrome B-mediated signaling. n6 -甲基腺苷解读子ECT1通过赤霉素酸和光敏色素b介导的信号传导调节种子萌发。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-12 DOI: 10.1093/plphys/kiaf180

Zenglin Li,Yuhang Ma,Wen Sun,Pengjun Ding,Yifan Bu,Yuhong Qi,Tingrui Shi,Chengchao Jia,Beilei Lei,Chuang Ma

{"title":"The N6-methyladenosine reader ECT1 regulates seed germination via gibberellic acid- and phytochrome B-mediated signaling.","authors":"Zenglin Li,Yuhang Ma,Wen Sun,Pengjun Ding,Yifan Bu,Yuhong Qi,Tingrui Shi,Chengchao Jia,Beilei Lei,Chuang Ma","doi":"10.1093/plphys/kiaf180","DOIUrl":"https://doi.org/10.1093/plphys/kiaf180","url":null,"abstract":"Seed germination, a pivotal stage in plant growth, is governed by phytohormones such as gibberellic acid (GA) and influenced by phytochromes, which are key photoreceptors in plants. The N6-methyladenosine (m6A) RNA modification is fundamental to plant growth and development. However, the molecular mechanisms underlying the regulation of PHYTOCHROME B (phyB) and the function of m6A signaling in GA-mediated seed germination remain elusive. Here, we discovered EVOLUTIONARILY CONSERVED C-TERMINAL REGION 1 (ECT1) as an m6A reader protein that directly binds to m6A and forms homodimers to enhance its stability in Arabidopsis (Arabidopsis thaliana). We observed that the ect1-1 mutant exhibits attenuated GA3 responsiveness in seed germination. Restoration of ECT1 function in ect1-1 confirmed the role of ECT1 in promoting seed germination. Our findings indicate that ECT1 promotes seed germination by destabilizing m6A-modified REPRESSOR OF GA1-3 1 (RGA1), a key inhibitor of GA-mediated seed germination. Moreover, ECT1 establishes a regulatory circuit with DOF AFFECTING GERMINATION 2 (DAG2), another regulator of GA-mediated seed germination. DAG2 directly binds to the ECT1 promoter and controls its transcription, and ECT1 modulates DAG2 mRNA stability through m6A binding. Furthermore, we identified PHYB as a common downstream target of DAG2 and ECT1. ECT1 binds directly to m6A-modified PHYB and influences its stability, and DAG2 binds to the PHYB promoter to regulate its transcription. Our findings demonstrate that ECT1 fine-tunes m6A-regulated seed germination via complex and multifaceted molecular mechanisms, particularly through interactions with GA and phyB, broadening our understanding of m6A-regulated processes in Arabidopsis.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"25 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143932713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Major Facilitator Superfamily (MFS) transporters balance sugar metabolism in peach. 主要促进转运体超家族（MFS）平衡桃糖代谢。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-12 DOI: 10.1093/plphys/kiaf192

Xuanwen Yang,Wenhua Yang,Jiacui Li,Changwen Chen,Siyu Chen,Huan Wang,Jinlong Wu,Hui Xue,Yuting Liu,Jianzhong Lu,Yiwen Wang,Mengrui Du,Yong Li,Weichao Fang,Ruirui Liu,Yanling Peng,Qiang Xu,Yongfeng Zhou,Lirong Wang,Ke Cao

{"title":"Major Facilitator Superfamily (MFS) transporters balance sugar metabolism in peach.","authors":"Xuanwen Yang,Wenhua Yang,Jiacui Li,Changwen Chen,Siyu Chen,Huan Wang,Jinlong Wu,Hui Xue,Yuting Liu,Jianzhong Lu,Yiwen Wang,Mengrui Du,Yong Li,Weichao Fang,Ruirui Liu,Yanling Peng,Qiang Xu,Yongfeng Zhou,Lirong Wang,Ke Cao","doi":"10.1093/plphys/kiaf192","DOIUrl":"https://doi.org/10.1093/plphys/kiaf192","url":null,"abstract":"Sugar content is a key determinant of peach (Prunus persica) fruit quality, influencing taste, consumer preferences, and market value. However, the roles of Major Facilitator Superfamily (MFS) transporters in sugar metabolism and regulation remain largely unexplored. This study employed a combination of spatial metabolomics, quantitative genetics, transcriptomics, comparative genomics, and functional genomics to investigate the role of 67 MFS members in balancing sugar metabolism during peach fruit development. Spatial metabolomics revealed dynamic sugar distribution patterns, with ERD6-like transporters (PpERDL16-1) and tonoplastic sugar transporters 1 (PpTST1) promoting sucrose accumulation and Polyol/monosaccharide transporters 5 (PpPMT5-1) and sucrose transporters 4 (PpSUT4) reducing sucrose transport during fruit ripening. Functional studies confirmed these roles: PpERDL16-1 overexpression enhanced sucrose transport, and PpPMT5-1 or PpSUT4 silencing reduced sugar levels in peach fruit. Quantitative trait locus (QTL) mapping identified a major locus on chromosome 5, upstream of PpTST1, forming distinct haplotypes (Hap1 and Hap2). Hap1 was associated with lower PpTST1 expression and higher sugar and soluble solids content (SSC), while Hap2 was linked to higher PpTST1 expression and lower sugar content. This inverse relationship suggests that upstream genetic variants fine-tune PpTST1 expression in a context-dependent manner, potentially through interactions with transcription factors or epigenetic modifiers. Notably, PpTST1 overexpression increased sugar content but did not alter SSC, indicating compensatory mechanisms such as changes in organic acid metabolism or water content. These results illuminate the molecular mechanisms regulating sugar homeostasis in peach fruits, providing valuable targets for the genetic improvement of fruit quality through breeding programs.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"32 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143932716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0