Plant Physiology最新文献_第2页

Integrating the tomato chlorosis virus with the tobacco rattle virus facilitates the direct infection of tomato. 将番茄萎黄病毒与烟草响尾蛇病毒结合，有利于番茄的直接侵染。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-04-30 DOI: 10.1093/plphys/kiag260

Ge Wang, Yumei Bian, Shuo Liu, Fengze Sun, Chenxu Niu, Peng Zhang, Xiaofei Qin, Jiucheng Zhang, Wenzheng Gao, Fangfang Ma, Zhilong Bao

{"title":"Integrating the tomato chlorosis virus with the tobacco rattle virus facilitates the direct infection of tomato.","authors":"Ge Wang, Yumei Bian, Shuo Liu, Fengze Sun, Chenxu Niu, Peng Zhang, Xiaofei Qin, Jiucheng Zhang, Wenzheng Gao, Fangfang Ma, Zhilong Bao","doi":"10.1093/plphys/kiag260","DOIUrl":"https://doi.org/10.1093/plphys/kiag260","url":null,"abstract":"Tomato chlorosis virus (ToCV) threatens tomato production, resulting in substantial yield losses. An efficient inoculation system for ToCV can accelerate the isolation and utilization of tomato-resistant genetic resources. However, infectious ToCV clones cannot directly infect tomato plants. In this study, we cloned the full-length ToCV genome, including RNA1 and RNA2 molecules, and integrated their corresponding DNA fragments separately into the less virulent tobacco rattle virus-based pTRV2GW vector. Agrobacterium suspensions carrying pTRV1, pTRV2GW-RNA1, and pTRV2GW-RNA2 plasmids were mixed in equal amounts as the ToCV infectious clones and introduced into tomato cotyledons via a needleless syringe. RT-PCR analysis detected RNA1 ORF3 and RNA2 ORF10 after infection, and the ToCV capsid protein was detected via western blotting. Transmission electron microscopy revealed the viral aggregates of ToCV in infected leaves. Plants with successful infection displayed typical disease symptoms, including stunted growth, yellow leaves, increased anthocyanin accumulation on the abaxial side, reduced photosynthetic and antioxidant capacities, decreased fruit size, and impaired fruit maturation. The ToCV infectious clones were applied to susceptible tomato cultivars and resistant wild species, and only susceptible cultivars developed typical disease symptoms. Western blotting showed lower viral accumulation in resistant species than in susceptible cultivars. Thus, integrating the ToCV genome with the less virulent TRV enables direct infection of tomato by ToCV, accelerating the breeding of ToCV-resistant tomato and creating a feasible strategy to establish highly efficient viral infectious clones in other systems.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147819524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ABA receptor NtPYL6 promotes flavonol biosynthesis to enhance tobacco resistance to UV-B. ABA受体NtPYL6促进黄酮醇生物合成，增强烟草抗UV-B能力。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag257

Zhong Wang,Li Xu,Haitao Huang,Yali Liu,Pingping Liu,Jiarui Jiang,Xin Xu,Huina Zhou,Qiansi Chen,Xuemei Li,Qian Gao,Jun Yang

{"title":"The ABA receptor NtPYL6 promotes flavonol biosynthesis to enhance tobacco resistance to UV-B.","authors":"Zhong Wang,Li Xu,Haitao Huang,Yali Liu,Pingping Liu,Jiarui Jiang,Xin Xu,Huina Zhou,Qiansi Chen,Xuemei Li,Qian Gao,Jun Yang","doi":"10.1093/plphys/kiag257","DOIUrl":"https://doi.org/10.1093/plphys/kiag257","url":null,"abstract":"ABA and flavonol accumulation protect plants against UV-B radiation damage. However, the molecular mechanism by which UV-B enhances ABA signaling to induce flavonol biosynthesis remains largely unknown. Here, we found that ABA receptor ntpyl6 (PYRABACTIN RESISTANCE 1-LIKE6) mutants are more sensitive to UV-B than the WT and that UV-B significantly induces ABA accumulation and NtPYL6 expression in tobacco (Nicotiana tabacum). The induction of UV-B on NtPYL6 expression was achieved by inactivating NtBES1 (BRI1-EMS-SUPPRESSOR1), which directly binds to the E-box present in the NtPYL6 promoter to inhibit its expression. Metabolome and transcriptome analyses demonstrated that NtPYL6 positively mediates flavonol accumulation and the expression of related genes induced by ABA signaling. We further showed that NtPYL6-NtABI1/NtHAB1-NtSnRK2.12 constitute the core ABA signaling pathway to activate NtABF3 (ABA-RESPONSIVE ELEMENT-BINDING FACTOR 3). NtABF3 directly binds to the ABRE cis-elements present in the promoters of NtCHS and NtFLS genes to induce their transcription, leading to the increased accumulation of flavonols and enhanced plant resistance to UV-B. Our results elucidate a mechanism by which enhanced ABA signaling promotes flavonol accumulation, thereby improving plant resistance to UV-B.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"29 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147754997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hornwort pyrenoids: a terrestrial exception with engineering lessons. 牛膝草：地球上有工程学经验的例外。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag247

Tanner A Robison, Juan Carlos Villarreal A, Fay-Wei Li, Laura H Gunn

{"title":"Hornwort pyrenoids: a terrestrial exception with engineering lessons.","authors":"Tanner A Robison, Juan Carlos Villarreal A, Fay-Wei Li, Laura H Gunn","doi":"10.1093/plphys/kiag247","DOIUrl":"https://doi.org/10.1093/plphys/kiag247","url":null,"abstract":"Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) underpins nearly all primary production yet remains a slow, error-prone enzyme because it catalyzes both carboxylation and oxygenation, the latter initiating photorespiration and reducing net carbon gain. Many organisms mitigate these limitations not by improving Rubisco selectivity for CO2 directly, but by modifying its local environment using CO₂-concentrating mechanisms (CCMs). In algae, a prominent biophysical CCM strategy is the pyrenoid: a phase-separated, Rubisco-rich condensate coupled to bicarbonate transport, local carbonic anhydrase activity, and diffusion barriers that elevate CO₂ at Rubisco active sites. Although pyrenoids have been most intensively studied in algal models, a pyrenoid-based CCM has evolved independently in a single land-plant lineage-the hornworts-providing a powerful comparative system for understanding how chloroplast organization can be tuned to terrestrial CO₂-delivery constraints. Here we synthesize a century of hornwort pyrenoid research in ecological, phylogenetic, and mechanistic context. We summarize bryophyte anatomical and microhabitat features that impose strong CO₂ diffusion limitation, and compare hornwort and algal pyrenoids in ultrastructure, molecular parts lists, and regulation. We highlight emerging models for hornwort pyrenoid formation, inorganic-carbon delivery, CO₂ generation and recapture, and recent biochemical/structural work revealing distinctive hornwort Rubisco properties and biogenesis. Finally, we discuss how hornwort pyrenoids complement efforts to engineer algal pyrenoid components into C₃ crops, and propose modular, hybrid engineering strategies that leverage hornwort compatibility with the embryophyte chloroplast, while selectively importing algal modules. Together, hornwort pyrenoids illustrate both the convergent logic and the lineage-specific solutions of biophysical CO₂ concentration, and they open avenues for mechanistic discovery and photosynthesis engineering.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147778378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

L-Glutamic acid negatively regulates extracellular ATP-induced reactive oxygen species signaling. l -谷氨酸负调控胞外atp诱导的活性氧信号。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag230

Ronald J Myers,Abdul Ghani,Sameep Dhakal,Trupti Joshi,Daewon Kim,Ranjita Sinha,Gary Stacey,Ron Mittler

{"title":"L-Glutamic acid negatively regulates extracellular ATP-induced reactive oxygen species signaling.","authors":"Ronald J Myers,Abdul Ghani,Sameep Dhakal,Trupti Joshi,Daewon Kim,Ranjita Sinha,Gary Stacey,Ron Mittler","doi":"10.1093/plphys/kiag230","DOIUrl":"https://doi.org/10.1093/plphys/kiag230","url":null,"abstract":"Extracellular ATP (eATP) and L-Glutamic acid (L-Glu) are important damage associated molecular pattern (DAMP) molecules released from cells during injury. Both molecules trigger wound-associated signal transduction pathways, as well as the enhanced production of reactive oxygen species (ROS) by the RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) protein. However, whether eATP and L-Glu trigger overlapping or distinct pathways is mostly unknown. Here we report that Arabidopsis (Arabidopsis thaliana) responses to eATP or L-Glu are distinct from each other in terms of tissue specificity and transcriptomic responses. Thus, although both DAMPs trigger the expression of multiple wounding and hormone response transcripts in systemic tissues, eATP and L-Glu induced transcripts have little overlap between them. We further show that wounding of different tissues may result in ROS responses that are controlled by different DAMP receptors. Thus, activation of ROS production following injury of non-vascular tissues primarily depended on the eATP receptors PURINORECEPTOR 2 KINASE 1 and 2 (P2K1P2K2), while activation of ROS responses in vascular tissues following injury primarily depended on the L-Glu receptors GLU-LIKE RECEPTORS 3.3 and 3.6 (GLR3.3GLR3.6). Interestingly, we found that in the absence of the GLR3.3GLR3.6 receptors (i.e., in the glr3.3glr3.6 double mutant), the ROS response to eATP application is enhanced. This finding suggests that the L-Glu pathway may suppress the eATP pathway during wounding. Taken together, our findings suggest that the DAMP molecules eATP and L-Glu have complex interactions that appear to be both partly complementary and partly antagonistic, as well as tissue dependent.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"33 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147754919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rorippa islandica is a genetically accessible dicot model system to study flooding tolerance. Rorippa islandica是一个遗传上可接近的dicot模型系统，用于研究洪水耐受性。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag254

Malte M Bartylla,Emma L R Düthorn,Jana T Müller,Markus Wirtz,Hans van Veen,Rashmi Sasidharan,Angelika Mustroph

{"title":"Rorippa islandica is a genetically accessible dicot model system to study flooding tolerance.","authors":"Malte M Bartylla,Emma L R Düthorn,Jana T Müller,Markus Wirtz,Hans van Veen,Rashmi Sasidharan,Angelika Mustroph","doi":"10.1093/plphys/kiag254","DOIUrl":"https://doi.org/10.1093/plphys/kiag254","url":null,"abstract":"Most crop species cannot survive prolonged flooding events. Within the Cardamineae tribe of the Brassicaceae family, several wild species display high flooding tolerance and are therefore attractive study systems to unravel tolerance mechanisms. However the genetic recalcitrance of many of these species has prevented detailed mechanistic studies of observed tolerance traits. Here, Rorippa islandica was identified as a genetically accessible diploid species with high submergence tolerance. Comparison of its submergence transcriptome with that of another diploid species from the same genus, the submergence sensitive R. stylosa, revealed a strong and partially overlapping transcriptomic response to 48 h submergence. It also revealed RiBCA3 as a potential tolerance gene contributing to the higher submergence survival of R. islandica. Successful CRISPR-Cas9-mediated knockout of RiBCA3 confirmed the suitability of this species for genetic transformation. However, although it was hypothesized that RiBCA3 might have an important function in carbon fixation under water, no differences in submergence survival or underwater photosynthesis were observed between wild-type and bca3 knockout lines. The molecular mechanisms of submergence tolerance of Rorippa islandica are therefore not yet understood. This work demonstrates the suitability of Rorippa islandica for molecular genetics studies and presents a promising dicot model for investigation of underlying tolerance mechanisms, especially for comparative studies with Arabidopsis. This species might contribute to knowledge on flood tolerance in dicots, particularly Brassica oilseed crops and vegetables, while current knowledge is based primarily on rice (a monocot) studies.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"72 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147754994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The bHLH transcription factor IBL1 fine-tunes growth and development via restraining cell elongation/expansion in Arabidopsis. bHLH转录因子IBL1通过抑制拟南芥细胞伸长/扩增来调控生长发育。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag248

Yuqi Qian, Tengyue Zhang, Xiaonuan Hao, Meng Li, Yike Xie, Puyuan Liu, Xu Wang, Yunjing Hao, Bingyao Li, Yaqi Hao, Min Xu, Aigen Fu

{"title":"The bHLH transcription factor IBL1 fine-tunes growth and development via restraining cell elongation/expansion in Arabidopsis.","authors":"Yuqi Qian, Tengyue Zhang, Xiaonuan Hao, Meng Li, Yike Xie, Puyuan Liu, Xu Wang, Yunjing Hao, Bingyao Li, Yaqi Hao, Min Xu, Aigen Fu","doi":"10.1093/plphys/kiag248","DOIUrl":"https://doi.org/10.1093/plphys/kiag248","url":null,"abstract":"bHLH proteins regulate plant development, hormone responses, and stress adaptation; however, the precise functions of most bHLHs remain unclear. In this study, we explored the functions and mechanism of a bHLH gene, IBH1-LIKE 1 (IBL1), in Arabidopsis. IBL1 negatively controlled root growth, hypocotyl elongation, and leaf expansion, as indicated by larger or longer cells in ibl1 compared to Col-0 in the early vegetative process. IBL1 promoted photomorphogenesis and participated in various hormone pathways. In later vegetative stages, ibl1 plants were smaller and weaker than Col-0. During the reproductive stage, the deficiency of IBL1 led to earlier bolting and unhealthy shoots but did not obviously impair gametogenesis and embryogenesis. Longer pistils occurred in ibl1 plants, while stamens exhibited normal length, indicating that IBL1 restricted pistil cell elongation. The unsynchronized sexual organs led to substantial incomplete pollination and vacant seats in siliques. Transcriptomic analysis implied that IBL1 functions via affecting the expression of cell expansion-related genes. Yeast two-hybrid, bimolecular fluorescence complementation, in vitro protein pull-down, electrophoretic mobility shift assays, and dual luciferase assays indicated that IBL1 interacts with other bHLHs, including PRE5, HBI1, and CIL2. These bHLH proteins could constitute a tri-antagonistic system to regulate plant cell elongation/expansion. In a proposed model, IBL1 and PRE5 antagonistically regulate the activity of HBI1/CIL2 to control target gene expression and eventually fine-tune cell elongation/expansion. This study reveals physiological roles and the working mode of IBL1 and provides insights into the regulation of plant growth and development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147778404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyrenoid-based CO2-concentrating mechanisms across diverse lineages: convergent features and their applications for engineering. 基于芘类的不同谱系的二氧化碳集中机制：收敛特征及其在工程中的应用。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag250

Jessica H Pritchard, Sophie N R Young, Herbie Garland, Luke C M Mackinder, Alistair J McCormick

{"title":"Pyrenoid-based CO2-concentrating mechanisms across diverse lineages: convergent features and their applications for engineering.","authors":"Jessica H Pritchard, Sophie N R Young, Herbie Garland, Luke C M Mackinder, Alistair J McCormick","doi":"10.1093/plphys/kiag250","DOIUrl":"https://doi.org/10.1093/plphys/kiag250","url":null,"abstract":"Engineering pyrenoid-based CO2-concentrating mechanisms (pCCMs) into crop plants is a promising strategy to boost photosynthetic performance, enhance yield potential, and improve resilience to future climates. Achieving this goal requires a deeper understanding of the molecular principles that enable pyrenoids to elevate CO2 around Rubisco. Although pyrenoids span a remarkable diversity of forms across algae and hornworts, they consistently exhibit three core features: a condensed Rubisco matrix, specialised membranes that deliver inorganic carbon, and a diffusion barrier that restricts CO2 leakage. Here, we review recent mechanistic advances from Chlamydomonas reinhardtii alongside emerging insights from other algae and hornworts that highlight both conserved strategies and lineage-specific innovations in Rubisco condensation, membrane-associated inorganic carbon channelling, and matrix encapsulation. Together, these findings refine our understanding of pCCM diversity and provide an increasingly robust blueprint for reconstructing pCCMs in vascular plant chloroplasts.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147778421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor MYB52 regulates salt stress tolerance in tomato by modulating ion homeostasis and proline biosynthesis. 转录因子MYB52通过调节离子稳态和脯氨酸生物合成调控番茄耐盐性。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag256

Yancui Di,Shihao Lou,Zichen Wang,Tao Wang,Xiaohui Niu,Zelin Ji,Weiyang Liu,Shuangchen Chen,Zhixin Guo,Shihan Zheng,Tao Zhang,Fengzhi Piao,Yong Wang,Xiaoxing Dong,Chaoyi Hu,Han Dong

{"title":"The transcription factor MYB52 regulates salt stress tolerance in tomato by modulating ion homeostasis and proline biosynthesis.","authors":"Yancui Di,Shihao Lou,Zichen Wang,Tao Wang,Xiaohui Niu,Zelin Ji,Weiyang Liu,Shuangchen Chen,Zhixin Guo,Shihan Zheng,Tao Zhang,Fengzhi Piao,Yong Wang,Xiaoxing Dong,Chaoyi Hu,Han Dong","doi":"10.1093/plphys/kiag256","DOIUrl":"https://doi.org/10.1093/plphys/kiag256","url":null,"abstract":"Salt stress severely affects tomato (Solanum lycopersicum L.) survival and growth. Although the involvement of the tomato MYB gene family in response to salt stress has been well established, the mechanism underlying resistance to salt stress remains unclear. In this study, we investigated the role of MYB52 in conferring salt stress resistance using overexpression and knockout tomato seedlings obtained via genetic modification. We demonstrated that MYB52 improves the ability of tomato to withstand salt stress by enhancing antioxidant capacity, photosynthetic capacity, and proline content while reducing relative electrolyte leakage (REL) levels. Transcription of MYB52 was induced by salt stress-induced ABA accumulation. Activated MYB52 bound to the promoter of Salt Overly Sensitive 1 (SOS1), Na+/H+ exchanger 1 (NHX1), pyrroline-5-carboxylate synthetase 1 (P5CS1) and Ornithine δ-aminotransferase (OAT), thereby positively regulating their expression. This regulation resulted in enhanced potassium (K+) absorption, sodium (Na+) efflux, and proline content, which contributed to improved salt tolerance in tomato. Furthermore, silencing of SOS1, NHX1, P5CS1 and OAT impaired the salt tolerance of the WT and MYB52-OE plants. These results will refine the mechanistic framework for MYB52 and accelerate its application in crop improvement under increasing soil salinization, thereby advancing the sustainable and efficient production of tomatoes and other vegetable crops.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"25 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147754993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histone H3K27 trimethylation modulates the cotton response to KCl-induced ionic stress. 组蛋白H3K27三甲基化调节棉花对氯化钾诱导的离子胁迫的反应。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag252

Zhiqiang Zhang, Wenqi Huo, Xinyang Li, Zhongying Ren, Yangai Liu, Kunlun He, Fei Zhang, Jinfeng Guo, Xiongfeng Ma, Daigang Yang, Wei Li

{"title":"Histone H3K27 trimethylation modulates the cotton response to KCl-induced ionic stress.","authors":"Zhiqiang Zhang, Wenqi Huo, Xinyang Li, Zhongying Ren, Yangai Liu, Kunlun He, Fei Zhang, Jinfeng Guo, Xiongfeng Ma, Daigang Yang, Wei Li","doi":"10.1093/plphys/kiag252","DOIUrl":"https://doi.org/10.1093/plphys/kiag252","url":null,"abstract":"Potassium (K⁺), an essential macronutrient for plant growth and stress adaptation, becomes physiologically stressful when overaccumulated in soil. While K fertilization enhances cotton (Gossypium hirsutum) fiber quality and yield, the consequential KCl-induced ionic stress has emerged as a critical agricultural challenge demanding molecular-level resolution. This study unveils the previously unexplored epigenetic mechanisms mediated by histone H3 lysine 27 trimethylation (H3K27me3) in cotton's adaptation to KCl stress. Through integrated cleavage under targets and tagmentation (CUT&Tag) chromatin profiling and transcriptome sequencing (RNA-seq), we demonstrate that KCl stress triggers genome-wide attenuation of H3K27me3 deposition, concomitant with characteristic stress phenotypes in cotton seedlings. Suppression of H3K27me3 using RDS 3434 significantly ameliorated KCl-induced physiological damage, thereby supporting a functional correlation between this epigenetic mark and stress tolerance. Mechanistic analyses revealed 48 genes exhibiting inverse correlation between H3K27me3 enrichment and transcriptional activation, including two that encode pivotal salt-tolerance regulators: Glutathione Synthase1 (GhGSH1) and Salt-Related MYB1 (GhSRM1). Virus-induced gene silencing (VIGS) validation confirmed these H3K27me3-associated genes as essential components of cotton's ionic stress response network. Our findings delineate the epigenetic landscape associated with KCl stress adaptation and highlight H3K27me3-mediated chromatin remodeling as a critical regulatory layer in plant abiotic stress responses. This work provides insights into epigenetic engineering strategies for developing stress-resilient cotton cultivars.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147778391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cytokinin-auxin antagonistic module participates in nitrogen-triggered tiller outgrowth in rice. 细胞分裂素-生长素拮抗模块参与氮触发水稻分蘖生长。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag258

Sourav Chatterjee,Aditi Dwivedi,Ananda K Sarkar,Aashish Ranjan

{"title":"A cytokinin-auxin antagonistic module participates in nitrogen-triggered tiller outgrowth in rice.","authors":"Sourav Chatterjee,Aditi Dwivedi,Ananda K Sarkar,Aashish Ranjan","doi":"10.1093/plphys/kiag258","DOIUrl":"https://doi.org/10.1093/plphys/kiag258","url":null,"abstract":"Tillering is a key trait that shapes rice (Oryza sativa L.) shoot architecture and directly influences yield. While tiller bud formation is largely genetically determined, bud outgrowth into functional tillers is highly responsive to environmental cues. However, integration of environmental signals with genetic regulators to determine tiller bud fate remains poorly understood. Here, we investigated the effects of nitrogen on early stages of tiller bud outgrowth. Comprehensive phenotyping and temporal transcriptomic analyses demonstrated that both nitrate and ammonium promote bud outgrowth and elicit overlapping transcriptional responses, with nitrate acting more slowly. Gene regulatory network analysis identified phytohormone signaling as a key interface for nitrogen- triggered tiller outgrowth. Pharmacological and molecular experiments demonstrated the involvement of cytokinin-auxin antagonism in nitrogen-mediated tillering. Cytokinin promoted bud activation by repressing the critical bud dormancy regulators rice TEOSINTE BRANCHED 1 (OsTB1) and a homolog of PIN-FORMED 1 (OsPIN1a) through the Cytokinin Response Factors OsERF53/54. In contrast, auxin maintained dormancy by inducing OsTB1 and OsPIN1a expression through Auxin Response Factors OsARF11/16. Consistently, OsTB1 overexpression lines showed reduced responsiveness to nitrogen and hormone treatments, placing OsTB1 downstream of these convergent inputs. Sequence and gene expression differences in OsERF53/54, along with phenotypic variations across contrasting rice accessions, further substantiated the crucial roles of OsERF53/54 in nitrogen-mediated tillering. Together, we identify a key regulatory role of the cytokinin-auxin antagonistic module for integrating nitrogen signals to determine tiller bud fate. Adequate nitrogen promotes cytokinin signaling while attenuating auxin signaling and transport in tiller buds, thereby releasing dormancy and initiating bud outgrowth.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147754992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0