Plant Physiology最新文献_第2页

Establishment of an efficient and versatile genome editing platform for L. ruthenicum 一种高效、通用的白乳草基因组编辑平台的建立

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-07 DOI: 10.1093/plphys/kiaf486

Ying Sun, Zheng Hong, Wenwen Wang, Hong Zhang, Xiang Ren, Ximan He, Tingting Kan, Yunfang Fan, Chong Wang, Youlong Cao, Hui Zhang

{"title":"Establishment of an efficient and versatile genome editing platform for L. ruthenicum","authors":"Ying Sun, Zheng Hong, Wenwen Wang, Hong Zhang, Xiang Ren, Ximan He, Tingting Kan, Yunfang Fan, Chong Wang, Youlong Cao, Hui Zhang","doi":"10.1093/plphys/kiaf486","DOIUrl":"https://doi.org/10.1093/plphys/kiaf486","url":null,"abstract":"Black goji berry (Lycium ruthenicum Murr.) is a valuable functional food and traditional medicinal plant owing to its rich content of anthocyanins, trace minerals, vitamins, and polysaccharides. However, limited genetic manipulation tools have hindered functional genomic studies and trait improvement in this species. In this study, we optimized the genetic transformation system for L. ruthenicum, achieving a remarkably high transformation efficiency of 95.4%. Based on this system, we developed a clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9)-mediated gene knockout approach, using the Arabidopsis U6 (AtU6) promoter to drive sgRNA expression and the cauliflower mosaic virus 35S (35S) promoter to drive Cas9 expression, achieving editing efficiencies of 68.8% at the phytoene desaturase (LrPDS) locus and 96.0% at the betaine aldehyde dehydrogenase (LrBADH2) locus. Furthermore, we established an adenine base editing (ABE) system using the ribosomal protein subunit 5A (RPS5A) promoter to drive tRNA adenine deaminase-8e (TadA-8e-nSpCas9) cassette expression, achieving an editing efficiency of 72.2% at the LrPDS locus. To broaden protospacer adjacent motif (PAM) compatibility, we introduced the PAM-relaxed variant SpRY, enabling successful A-to-G editing at an acetolactate synthase (LrALS) target site containing a non-canonical NAN PAM, with an efficiency of 5.3%. Additionally, we developed a multiplex ABE system based on the tRNA-processing strategy, which enabled simultaneous editing at two independent loci with an efficiency of 33.3%. Our study establishes a robust genome editing toolkit for L. ruthenicum, offering valuable tools for functional gene analysis and molecular breeding in this economically important species.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"10 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145235530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A T-DNA-induced chromosomal translocation in smo2-2 disrupts gametophyte development independently of sterol biosynthesis. t - dna诱导的smo2-2染色体易位可独立于甾醇生物合成干扰配子体发育。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-07 DOI: 10.1093/plphys/kiaf497

Chao Tan,Huiwen Ren,Shuangli Sun,Wenhui Wang,Shuzhen Men

{"title":"A T-DNA-induced chromosomal translocation in smo2-2 disrupts gametophyte development independently of sterol biosynthesis.","authors":"Chao Tan,Huiwen Ren,Shuangli Sun,Wenhui Wang,Shuzhen Men","doi":"10.1093/plphys/kiaf497","DOIUrl":"https://doi.org/10.1093/plphys/kiaf497","url":null,"abstract":"The sterol C-4 demethylase multienzyme complex (SC4DM), containing the sterol 4α-methyl oxidases SMO1 and SMO2, plays essential roles in plant reproduction. In Arabidopsis (Arabidopsis thaliana), two SMO2 genes (SMO2-1 and SMO2-2) encode functionally redundant enzymes, as evidenced by the embryo-lethal phenotype and auxin-related patterning defects observed in smo2-1 smo2-2 double mutants. Intriguingly, while smo2-1/+ smo2-2 plants develop normally, smo2-1 smo2-2/+ heterozygotes produce siliques with approximately 50% unfertilized ovules, suggesting a genotype-dependent effect whose mechanism requires elucidation. Here, we found that the SMO2 genes are highly expressed during anther and ovule development, with SMO2-1 showing stronger expression than SMO2-2. The smo2-1 smo2-2/+ mutant exhibits ∼50% abortion rates for both male and female gametophytes, with developmental arrest occurring around the first mitosis. Through comprehensive analysis, we excluded several potential mechanisms for these aberrant phenotypes, including auxin deficiency, SMO2-2 splice variants, and secondary T-DNA insertions. Instead, we identified a reciprocal chromosomal translocation induced by T-DNA insertions in smo2-2 as the underlying cause. This conclusion was further supported by characterizing a knockdown allele (smo2-1 smo2-2a/+) and a CRISPR/Cas9 knockout line (smo2-1 smo2-2k1/+), which confirmed that the observed gametophytic defects are independent of SMO2 loss-of function. These findings highlight the necessity of analyzing multiple independent alleles when interpreting mutant phenotypes.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"11 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145241123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsS40-14 regulates rice seed germination by modulating ABA responsiveness and inhibiting dormancy release. OsS40-14通过调节ABA响应性和抑制休眠释放来调控水稻种子萌发。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-07 DOI: 10.1093/plphys/kiaf492

Xiangzi Zheng,Weiqi Wang,Chunlan Fan,Min Tan,Huiling Dai,Dongmei Liao,Zhuoning Dou, Habiba,Sirong Chen,Wuqiang Hong,Xiaowei Wang,Chuheng Lin,Xiaoli Wang,Ying Miao

{"title":"OsS40-14 regulates rice seed germination by modulating ABA responsiveness and inhibiting dormancy release.","authors":"Xiangzi Zheng,Weiqi Wang,Chunlan Fan,Min Tan,Huiling Dai,Dongmei Liao,Zhuoning Dou, Habiba,Sirong Chen,Wuqiang Hong,Xiaowei Wang,Chuheng Lin,Xiaoli Wang,Ying Miao","doi":"10.1093/plphys/kiaf492","DOIUrl":"https://doi.org/10.1093/plphys/kiaf492","url":null,"abstract":"Seed germination is a complex process influenced by various physical and biochemical factors, and dormancy release upon imbibition is essential for germination. However, the specific regulators controlling dormancy release in rice remain largely unknown. Here, we identified OsS40-14, a member of the senescence-associated S40 gene family, as a negative regulator of seed germination in rice. Using CRISPR/Cas9 gene editing and transgenic overexpression (OE) lines, we found that the oss40-14 mutant lines exhibit pre-harvest sprouting (PHS) and accelerated germination, while the OE-OsS40-14 lines show delayed germination. Notably, dormancy-breaking treatments largely eliminated these phenotypic differences. Analysis of endogenous abscisic acid (ABA) levels and the response to exogenous ABA revealed that OsS40-14 regulates germination by modulating ABA signaling. Transcriptomic and CUT & Tag-seq analyses demonstrated that OsS40-14 directly represses the transcription of two ABA catabolic genes, OsABA8ox1 and OsABA8ox3, thereby preventing the reduction of ABA levels during seed dormancy release. These findings establish OsS40-14 as a key regulator of seed germination through its control of ABA homeostasis, providing potential targets for breeding PHS-resistant varieties.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"9 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of enzymes VvNCED1 and VvCCD1 in β-Damascenone biosynthesis: insights into aroma development in grapes. VvNCED1和VvCCD1酶在β-大马士革酮生物合成中的作用：对葡萄香气发育的见解。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-07 DOI: 10.1093/plphys/kiaf496

Xiao Meng,Xuechen Yao,Yi Wei,Yachen Wang,Huimin Zhang,Keji Yu,Yibin Lan,Changqing Duan,Guangfeng Wu,Qiuhong Pan

{"title":"Roles of enzymes VvNCED1 and VvCCD1 in β-Damascenone biosynthesis: insights into aroma development in grapes.","authors":"Xiao Meng,Xuechen Yao,Yi Wei,Yachen Wang,Huimin Zhang,Keji Yu,Yibin Lan,Changqing Duan,Guangfeng Wu,Qiuhong Pan","doi":"10.1093/plphys/kiaf496","DOIUrl":"https://doi.org/10.1093/plphys/kiaf496","url":null,"abstract":"Grape (Vitis vinifera L.) is a widely cultivated fruit with significant economic value. β-Damascenone, a key norisoprenoid compound, contributes to the distinctive floral and fruity aroma of grape berries. However, the biosynthesis of this compound remains unresolved. Integrated transcriptomic and metabolomic analyses across 17 developmental stages of V. vinifera cv. Cabernet Sauvignon revealed stage-dependent regulation of aroma metabolism. Notably, norisoprenoid accumulation peaked at veraison, with the expression of both 9-cis-EPOXYCAROTENOID DIOXYGENASE 1 (VvNCED1) and CAROTENOID CLEAVAGE DIOXYGENASE 1 (VvCCD1) exhibiting a synchronized temporal pattern. Exogenous ABA treatment enhanced norisoprenoid accumulation and the expression of VvNCED1, NEOXANTHIN SYNTHASE (VvNXS), and CAROTENOID CLEAVAGE DIOXYGENASE 4b (VvCCD4b). Enzymatic assays using recombinant VvNCED1 demonstrated its capacity to cleave 9'-cis-neoxanthin in vitro, generating putative C25-allenic-apo-aldehyde and xanthoxin (an ABA precursor). Subsequent in vitro reactions with recombinant VvCCD1 converted C25-allenic-apo-aldehyde into grasshopper ketone, which undergoes conversion to β-damascenone. We propose two complementary pathways: 1) ABA-mediated regulation: VvNCED1-driven ABA biosynthesis upregulates norisoprenoid biosynthetic genes; 2) Direct precursor channeling: VvNCED1 and VvCCD1 sequentially process 9'-cis-neoxanthin into β-damascenone precursors, with grape-specific acidic conditions or uncharacterized enzymes facilitating final conversion. This collaborative VvNCED1-VvCCD1 model bridges carotenoid metabolism, ABA metabolism, and aroma development, providing insights into the spatiotemporal coordination of berry maturation and flavor compound accumulation.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"58 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145241088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fight smarter, not harder: NPR1-Mediated Immune Balance in Citrus Greening Disease. 更聪明地战斗，而不是更艰难：npr1介导的柑橘绿化病免疫平衡。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-07 DOI: 10.1093/plphys/kiaf479

Ritu Singh,Marcella Teixeira

引用次数: 0

The endoplasmic reticulum-associated degradation machinery selectively degrades stress-induced TIN1 during stress recovery 内质网相关的降解机制在应力恢复过程中选择性地降解应力诱导的TIN1

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-07 DOI: 10.1093/plphys/kiaf489

Yi Wang, Zhihui Ma, Jiarui Wu, Congcong Zhang, Yongwu Chen, Liangguang Lin, Juan Mao, Jianjun Zhang, Linchuan Liu, Pengcheng Wang, Jianming Li

{"title":"The endoplasmic reticulum-associated degradation machinery selectively degrades stress-induced TIN1 during stress recovery","authors":"Yi Wang, Zhihui Ma, Jiarui Wu, Congcong Zhang, Yongwu Chen, Liangguang Lin, Juan Mao, Jianjun Zhang, Linchuan Liu, Pengcheng Wang, Jianming Li","doi":"10.1093/plphys/kiaf489","DOIUrl":"https://doi.org/10.1093/plphys/kiaf489","url":null,"abstract":"The unfolded protein response (UPR) signaling pathway is activated by accumulation of misfolded proteins in the endoplasmic reticulum (ER) and stimulates production of ER chaperones to restore ER proteostasis. However, how UPR-induced proteins return to their pre-stress levels upon removal of ER stress remains unknown. TUNICAMYCIN-INDUCED1 (TIN1) is an Arabidopsis (Arabidopsis thaliana) protein that is normally expressed in pollen but is rapidly induced by ER stresses in vegetative tissues. Here we show that the ER stress-induced TIN1 is rapidly degraded in the UPR recovery phase. We found that TIN1 degradation depends on its asparagine-linked glycans and requires both EMS-mutagenized bri1 suppressor 5 (EBS5) and EBS6 for its recruitment to the ER-associated degradation (ERAD) complex. Loss-of-function mutations in the core component of this Arabidopsis ERAD complex greatly stabilize TIN1. Interestingly, two other UPR-induced proteins that are coexpressed with TIN1 remained stable upon ER stress removal, suggesting that rapid degradation during the stress-recovery phase likely applies to a subset of UPR-induced proteins. Further investigation is needed to uncover the mechanisms by which the ERAD machinery selectively degrades UPR-induced ER proteins.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145235327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive profiling of the epitranscriptome and translatome in rice seedlings under salt stress. 盐胁迫下水稻幼苗表转录组和翻译组的综合分析。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-06 DOI: 10.1093/plphys/kiaf481

Qian Qian,Yiming Zhou,Yuli Gan,Yongqing Ling,Jiantao Tan,Mengyuan Sheng,Xin Peng,Degui Zhou,Zhonghui Zhang,Qi Liu

{"title":"Comprehensive profiling of the epitranscriptome and translatome in rice seedlings under salt stress.","authors":"Qian Qian,Yiming Zhou,Yuli Gan,Yongqing Ling,Jiantao Tan,Mengyuan Sheng,Xin Peng,Degui Zhou,Zhonghui Zhang,Qi Liu","doi":"10.1093/plphys/kiaf481","DOIUrl":"https://doi.org/10.1093/plphys/kiaf481","url":null,"abstract":"Plants dynamically regulate gene expression at multiple levels, including transcription, splicing, polyadenylation, modification, and translation, to adapt to environmental changes. However, comprehensive studies exploring the epitranscriptome and translatome in response to salt stress in rice (Oryza sativa) remain limited. In this study, we performed nanopore direct RNA sequencing (DRS) and ribosome profiling to investigate the post-transcriptional and translational landscapes of rice seedlings (Nipponbare) under salt stress. Time-course transcriptome data revealed that differentially expressed genes uniquely identified 8 hours post-salt treatment were significantly enriched in Gene Ontology terms related to RNA processing and translation. DRS analysis showed that the global N6-methyladenosine (m6A) ratio decreased, while the N5-methylcytosine (m5C) ratio increased during early salt stress. Genes with significant changes in transcript abundance and RNA modifications were both enriched in oxidation-reduction processes. Notably, we found that the transcript abundance of modified genes exhibited a significant positive correlation with m5C ratios and a negative correlation with m6A ratios, particularly in oxidoreductase activity-related genes. Ribosome profiling demonstrated that the translation efficiency of modified mRNAs was significantly increased under early salt stress. Furthermore, we identified 2,078 transcripts with differential poly(A) tail length (PAL), with an increased number of transcripts exhibiting increased PAL. Further analysis revealed that the PAL of modified transcripts increased after salt treatment. These results broaden our understanding of the intricate interplay among transcript abundance, RNA modification, PAL, and translation under early salt stress in rice.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"55 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benzoic and salicylic acids inhibit β-substituted alanine synthase 4;1 in common bean. 苯甲酸和水杨酸抑制β-取代丙氨酸合成酶4；1在普通豆。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-06 DOI: 10.1093/plphys/kiaf485

Zixuan Lu,Wojciech Witek,Milosz Ruszkowski,Barbara Imiolczyk,Nataliya Paulish,Jaya Joshi,Mariusz Jaskolski,Frédéric Marsolais

{"title":"Benzoic and salicylic acids inhibit β-substituted alanine synthase 4;1 in common bean.","authors":"Zixuan Lu,Wojciech Witek,Milosz Ruszkowski,Barbara Imiolczyk,Nataliya Paulish,Jaya Joshi,Mariusz Jaskolski,Frédéric Marsolais","doi":"10.1093/plphys/kiaf485","DOIUrl":"https://doi.org/10.1093/plphys/kiaf485","url":null,"abstract":"The nutritionally essential sulfur amino acids, methionine and cysteine, are present at suboptimal levels in legumes, such as common bean (Phaseolus vulgaris L.). β-Substituted alanine synthase 4;1 (BSAS4;1) is the major isoform of cytosolic cysteine synthase present in the developing seeds of common bean. There is evidence that in addition to cysteine, this enzyme is also involved in the biosynthesis of the non-protein amino acid S-methylcysteine, which accumulates in the form of a γ-glutamyl dipeptide. Here, we report the high-resolution structure of recombinant BSAS4;1. Unexpectedly, the crystal structure showed the presence of a molecule of benzoic acid near the active site, which appeared to have been co-purified from E. coli. Kinetic analysis indicated that benzoic acid acts as a competitive inhibitor of BSAS4;1 with respect to O-acetylserine. IC50 values for benzoic acid and the structurally related salicylic acid were both equal to 0.6 mM. Using developing cotyledons grown in vitro, quantification of the incorporation of 13C3- and 15N-labeled serine into cysteine and downstream metabolites indicated that benzoic acid effectively inhibited cysteine biosynthesis in vivo at a concentration of 1.2 mM. The results of experiments tracking the incorporation of 13C-labeled sodium thiomethoxide provided further evidence that BSAS4;1 may be involved in the formation of free S-methylcysteine, through the condensation of O-acetylserine with methanethiol.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"28 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

More is not always merrier: enhanced atmospheric CO2 reduces nutritional seed quality in oak trees. 越多并不总是越好：大气中二氧化碳的增加降低了橡树种子的营养质量。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-05 DOI: 10.1093/plphys/kiaf491

Gunjan Sharma

引用次数: 0

Vegetative to generative1 (Vgt1) is an enhancer affecting flowering time and jasmonate signaling in maize by promoting the expression of Zea mays Related to APETALA 2.7 Vgt1 （Vegetative to generative1）是一种通过促进与aptala相关的玉米基因表达而影响玉米开花时间和茉莉酸信号转导的促进剂

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-03 DOI: 10.1093/plphys/kiaf468

Johan Zicola, Blaise Weber, Xiaoyu Tu, Rechien Bader, Dimitrios Zisis, Stijn Aesaert, Silvio Salvi, Pawel Krajewski, Mieke Van Lijsebettens, Chuanshun Li, Yangmeihui Li, Silin Zhong, Stefan Scholten, Franziska Turck, Maike Stam

{"title":"Vegetative to generative1 (Vgt1) is an enhancer affecting flowering time and jasmonate signaling in maize by promoting the expression of Zea mays Related to APETALA 2.7","authors":"Johan Zicola, Blaise Weber, Xiaoyu Tu, Rechien Bader, Dimitrios Zisis, Stijn Aesaert, Silvio Salvi, Pawel Krajewski, Mieke Van Lijsebettens, Chuanshun Li, Yangmeihui Li, Silin Zhong, Stefan Scholten, Franziska Turck, Maike Stam","doi":"10.1093/plphys/kiaf468","DOIUrl":"https://doi.org/10.1093/plphys/kiaf468","url":null,"abstract":"Transcriptional enhancers participate in cell and tissue differentiation in all multicellular organisms. Here, we characterized the candidate enhancer Vegetative to generative1 (Vgt1), a major QTL for flowering time in maize. Transgenic lines containing an inverted repeat that induces DNA methylation at Vgt1 showed early flowering and an accelerated growth rate during early development. DNA methylation of Vgt1 was associated with the downregulation of the AP2-like floral repressor ZmRap2.7 in specific leaf tissues at the early stages of maize development. In line with Vgt1 regulating ZmRap2.7, chromosome conformation capture data showed that Vgt1 physically interacts with the ZmRap2.7 transcription start site. Finally, chromatin immunoprecipitation of transiently expressed ZmRap2.7 in protoplasts indicated that this transcription factor binds to the promoters of several hundred genes. These genes include many genes that are differentially expressed in maize lines with and without extra DNA methylation at Vgt1. Altogether, we show that ZmRap2.7 is transcriptionally controlled by Vgt1 and is involved in regulating flowering time and other biological pathways, such as jasmonate signaling.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"197 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145215921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0