Plant Physiology最新文献_第10页

Gibberellin-dependent brassinosteroid signaling modulates plant growth by optimizing nitrogen levels during salinity stress 赤霉素依赖的油菜素类固醇信号通过优化盐胁迫下的氮水平来调节植物生长

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-25 DOI: 10.1093/plphys/kiag720

Shreya Gupta, Ritesh Kumar Yadav, Anshika Pandey, Nidhi Gandhi, Loitongbam Lorinda Devi, Subham Agarwal, Kratika Singh, Sonali Sen, Muhammed Shamnas v, Subodh Kumar Sinha, Amar Pal Singh

{"title":"Gibberellin-dependent brassinosteroid signaling modulates plant growth by optimizing nitrogen levels during salinity stress","authors":"Shreya Gupta, Ritesh Kumar Yadav, Anshika Pandey, Nidhi Gandhi, Loitongbam Lorinda Devi, Subham Agarwal, Kratika Singh, Sonali Sen, Muhammed Shamnas v, Subodh Kumar Sinha, Amar Pal Singh","doi":"10.1093/plphys/kiag720","DOIUrl":"https://doi.org/10.1093/plphys/kiag720","url":null,"abstract":"Low nitrogen (LN) and high salt stress are major constraints for plant growth and development. Under LN stress, plants typically show root elongation to increase nitrogen foraging from the rhizosphere, whereas high salt levels cause inhibition of root growth to avoid salinity stress-induced toxicity. To date, mechanisms and strategies of root adaptations in plants under combined LN and high salt stress remain poorly defined. Here, we provide evidence that Arabidopsis plants under LN stress show increased brassinosteroid (BR) signaling activity, which in turn suppresses salt stress-responsive pathways but improves nitrogen foraging. BR signaling, through its transcriptional regulators, activates and represses nitrogen and salt-responsive genes, respectively. In contrast, salinity stress represses gibberellin (GA) signaling and leads to the accumulation of DELLA proteins. High levels of DELLAs inhibit BZR1-dependent nitrogen-responsive growth to facilitate plant adaptation under high salt stress condition. Our data suggest that GA-promoted root elongation under combined LN and high salt stress is associated with BZR1-DELLA and Salt Overly Sensitive 3 (SOS3) protein stoichiometry. Specifically, DELLAs interact with SOS3, and high levels of SOS3 in turn facilitate tolerance to salt stress by adjusting DELLA-dependent BZR1 function and nitrogen homeostasis. Taken together, our findings highlight the adaptive responses plants exert to mitigate variable nitrogen and high salt stress conditions and appear essential for balancing growth and stress response.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149033085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sprouts of Plant Ecophysiology Along 100 Years of Plant Physiology 植物生态生理学的芽

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-23 DOI: 10.1093/plphys/kiag717

Alicia V Perera-Castro, Jaume Flexas

引用次数: 0

DcmiR172c–DcNAC41–DcHSFA2d/DcHSP70 module collaborates with DcGOX1 to modulate thermotolerance in carnation DcmiR172c-DcNAC41-DcHSFA2d /DcHSP70模块与DcGOX1协同调节康乃馨的耐热性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-23 DOI: 10.1093/plphys/kiag718

Ziyi Liu, Yuxin Jing, Pengcheng Xue, Xiaohan Zhang, Xiaojing Zhang, Xiaoni Zhang, Xinqiang Jiang, Xueli Wan

{"title":"DcmiR172c–DcNAC41–DcHSFA2d/DcHSP70 module collaborates with DcGOX1 to modulate thermotolerance in carnation","authors":"Ziyi Liu, Yuxin Jing, Pengcheng Xue, Xiaohan Zhang, Xiaojing Zhang, Xiaoni Zhang, Xinqiang Jiang, Xueli Wan","doi":"10.1093/plphys/kiag718","DOIUrl":"https://doi.org/10.1093/plphys/kiag718","url":null,"abstract":"Heat stress (HS) driven by global warming poses a challenge to the productivity and aesthetic value of carnation. To explore the molecular basis of thermotolerance, this study conducted integrated multi-omics analyses under HS, identifying a heat-responsive microRNA, DcmiR172c, whose expression exhibited an inverse relationship with the NAC transcription factor gene DcNAC41. Functional assays suggested that elevated DcmiR172c levels were associated with increased heat-induced membrane peroxidation and photosynthetic damage. Bioinformatic prediction, qRT-PCR analysis, dual-luciferase reporter assays, and protein-level experiments indicated that DcmiR172c may post-transcriptionally repress DcNAC41. In contrast, DcNAC41 acts as a transcriptional activator potentially involved in heat adaptation. It bound to and activated the promoters of the heat shock transcription factor DcHSFA2d and the heat shock protein gene DcHSP70-5, suggesting its involvement in the canonical HSF-HSP defense pathway. Notably, DcHSFA2d further amplified this response by activating DcHSP70-5, implying a possible feed-forward regulatory circuit. Moreover, protein interaction screening identified the glycolate oxidase DcGOX1 as a novel binding partner of DcNAC41. This interaction, supported by multiple in vivo and in vitro assays, appeared to enhance the transcriptional activity of DcNAC41 toward its targets, and contribute to heat tolerance. Collectively, this research outlines a multi-tiered regulatory framework in which a miRNA-guided NAC module coordinates with the HSF-HSP network and a metabolic enzyme to modulate heat acclimation. These findings highlight the potential importance of the DcmiR172c-DcNAC41-DcHSFA2d/DcHSP70-5 axis and offer valuable genetic targets for engineering climate-resilient carnation varieties.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"56 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary origin of cross-laminated cell walls predates lignification in vascular plant supporting tissues 在维管植物的支持组织中，交叉层压细胞壁的进化起源早于木质素化

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-23 DOI: 10.1093/plphys/kiag709

Naoki Takata

{"title":"Evolutionary origin of cross-laminated cell walls predates lignification in vascular plant supporting tissues","authors":"Naoki Takata","doi":"10.1093/plphys/kiag709","DOIUrl":"https://doi.org/10.1093/plphys/kiag709","url":null,"abstract":"Vertical elongation in land plants is a primary adaptive strategy driven by competitive pressures for increased height, conferring significant advantages for photosynthesis and long-distance dispersal. In these lineages, structural integrity is primarily mediated by the deposition of thick extracellular cell walls. In seed plants, these walls often exhibit a cross-laminated architecture, defined by cellulose microfibrils arranged at alternating angles, and lignin reinforcement, leveraging structural anisotropy to enhance mechanical resilience. However, the evolutionary chronology of the acquisition of these traits in basal vascular plant lineages is poorly understood. To investigate this question, we performed comprehensive histological and histochemical profiling of 35 basal vascular plant species, comprising eight lycophytes and 27 monilophytes, to elucidate the emergence of cross-laminated cell walls and their subsequent lignification in supporting tissues. In all examined taxa, sterome tissue, which is typically located in subepidermal regions, exhibited thick cell walls with a distinct cross-laminated architecture. Although lignification was ubiquitous in tracheary elements, its presence in sterome tissue showed significant interspecific variation. Most examined taxa possessed lignin-reinforced steromes, while specific lineages relied on nonlignified structural reinforcements. Quantitative morphometric analysis of sterome tissues and cells demonstrated that the mechanical contribution of steromes was depended on species-specific body plans. The findings suggest that the ability to form cross-laminated cell walls is a fundamental innovation that likely emerged before or independently of lignification in the supporting tissues of vascular plants. This study further highlights the functional significance of cell wall patterning in the successful colonization of terrestrial environments by basal plant lineages.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"40 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CmHSFA3-CmBRC1a-CmHB40 module inhibits axillary bud development in response to high temperature CmHSFA3-CmBRC1a-CmHB40模块抑制高温下腋芽发育

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-23 DOI: 10.1093/plphys/kiag668

Jinyu Jin, Yuqing Zhu, Shaocong Chen, Jingxuan Ye, Jiayu Xue, Fadi Chen, Weimin Fang, Aiping Song

{"title":"CmHSFA3-CmBRC1a-CmHB40 module inhibits axillary bud development in response to high temperature","authors":"Jinyu Jin, Yuqing Zhu, Shaocong Chen, Jingxuan Ye, Jiayu Xue, Fadi Chen, Weimin Fang, Aiping Song","doi":"10.1093/plphys/kiag668","DOIUrl":"https://doi.org/10.1093/plphys/kiag668","url":null,"abstract":"Branching is a key determinant of plant architecture, directly influencing growth, yield, and resource allocation. High temperature (HT) inhibits plant growth and development, often leading to alterations in plant architecture. However, the molecular mechanisms underlying temperature-induced inhibition of branching remain elusive. Here, we discovered that CmBRC1a is strongly induced by HT and inhibits axillary bud development in chrysanthemum. We further demonstrated that the heat shock transcription factor CmHSFA3 directly activates CmBRC1a by binding to its promoter, thus acting as its upstream regulator. Overexpression of CmHSFA3 further confirmed its role in suppressing branching. The finding that HT cannot completely suppress the enhanced branching phenotype of CmBRC1a knockdown lines suggests that HT-regulated branching is not entirely dependent on CmBRC1a. Similar to previously reported BRC1-mediated pathways, CmBRC1a directly activates the expression of CmHB40, thereby increasing abscisic acid (ABA) accumulation, which ultimately inhibits shoot branching. Our findings reveal CmHSFA3-CmBRC1a-CmHB40 module inhibits axillary bud development in response to HT, providing new insights into how environmental cues shape plant architecture.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"41 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149021255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SOBIR1 Integrates Pathogen Recognition and Hormonal Metabolism in Nicotiana tabacum SOBIR1整合了烟草病原体识别和激素代谢

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-23 DOI: 10.1093/plphys/kiag707

Adam Janotík, Martina Zapletalová, Karim Bouhidel, Jérôme Fromentin, Markéta Lišková, David Novák, Ivan Petřík, Ondřej Novák, Jaromir Mikulik, Jan Strnad, Ján Šmeringai, Markéta Pernisová, Nathalie Leborgne-Castel, Jan Lochman

{"title":"SOBIR1 Integrates Pathogen Recognition and Hormonal Metabolism in Nicotiana tabacum","authors":"Adam Janotík, Martina Zapletalová, Karim Bouhidel, Jérôme Fromentin, Markéta Lišková, David Novák, Ivan Petřík, Ondřej Novák, Jaromir Mikulik, Jan Strnad, Ján Šmeringai, Markéta Pernisová, Nathalie Leborgne-Castel, Jan Lochman","doi":"10.1093/plphys/kiag707","DOIUrl":"https://doi.org/10.1093/plphys/kiag707","url":null,"abstract":"The receptor-like kinase SOBIR1 (SUPPRESSOR OF BIR1) is a core component of immune receptor complexes mediating plant responses to extracellular immunogenic signals. While its role in pattern-triggered immunity is well defined in model species, its broader physiological functions remain largely unexplored. Here, we generated CRISPR-Cas9-derived double knockouts of two homeologous SOBIR1 genes in allotetraploid Nicotiana tabacum. The resulting Nt sobir1 double mutants failed to trigger elicitor-induced cell death in response to elicitins from Phytophthora and Pythium spp. but retained an attenuated early reactive oxygen species (ROS) response while lacking sustained ROS accumulation, showing that the transient and sustained ROS outputs differ in their dependence on SOBIR1. Functionally, the mutants showed compromised resistance to Phytophthora parasitica but unaltered responses to Botrytis cinerea and Pseudomonas syringae, pointing to pathogen-specific SOBIR1 roles. Beyond immunity, SOBIR1 appears to interface with developmental signaling modules involving CLAVATA2 and ERECTA–YODA, implicating it in phytohormone signaling, meristem regulation, and cell proliferation. Notably, Nt sobir1 mutants displayed increased hypocotyl cell wall stiffness despite transcriptional upregulation of wall-loosening genes, suggesting compensatory cell wall remodeling and perturbed integrity signaling. Transcriptome and phytohormone profiling revealed tissue-specific expression changes and altered levels of cytokinins, salicylic acid, jasmonic acid, and their conjugates. These findings position SOBIR1 as a multifunctional regulatory hub that links immune perception with phytohormone dynamics and cell wall mechanics in N. tabacum, expanding our understanding of co-receptor signaling plasticity across plant lineages.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"36 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant Peptide Hormones: Three Decades of Discovery and Emerging Paradigms 植物肽激素：三十年的发现和新兴范式

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-23 DOI: 10.1093/plphys/kiag699

Keiko U Torii

{"title":"Plant Peptide Hormones: Three Decades of Discovery and Emerging Paradigms","authors":"Keiko U Torii","doi":"10.1093/plphys/kiag699","DOIUrl":"https://doi.org/10.1093/plphys/kiag699","url":null,"abstract":"Plant peptide hormones have reshaped our understanding of intercellular signaling in plants. Once considered absent in plants, peptide-based signaling pathways emerged as key regulators of plant growth, development, environmental responses and immunity. A paradigm shift occurred with the discoveries of Systemin and Phytosulfokine (PSK) in the 1990s as well as the identifications of receptor-like kinases (RLKs) regulating important biological processes. Over the past three decades, a repertoire of peptide hormone families has been expanded enormously, with deeper understandings of peptide modifications, cleavage, modes of actions, and receptor binding and signal transduction. In this review, I highlight selected peptide hormone families, Systemin, PSK, INFLORESCENCE DEFICIENT IN ABSCISSION (IDA)/IDA-LIKES, CLAVATA3/EMBRYO SURROUNDING REGION (CLE), and EPIDERMAL PATTERNING FACTORs (EPF)/EPF-LIKES, and discuss how individual discoveries of the peptides and their cognate receptors have shaped emerging concepts in plant peptide signaling. Celebrating this Centennial Issue, emphasis will be given on contributions published in Plant Physiology. Finally, I discuss how expanding genomic resources and advanced technologies will accelerate the discovery and engineering of peptide signaling pathways to enhance plant resilience.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"2 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149021260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Grass-Specific Structural Myosin VIII Regulates Protoxylem Development and Hydraulic Conductance in Sorghum 草特异性结构肌球蛋白VIII调控高粱原木质部发育和水力导度

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-22 DOI: 10.1093/plphys/kiag712

Zhiyuan Liu, Ran Tian, Leonidas D'Agostino, Zhanguo Xin, Junping Chen, Gunvant Baliram Patil, Yinping Jiao

{"title":"A Grass-Specific Structural Myosin VIII Regulates Protoxylem Development and Hydraulic Conductance in Sorghum","authors":"Zhiyuan Liu, Ran Tian, Leonidas D'Agostino, Zhanguo Xin, Junping Chen, Gunvant Baliram Patil, Yinping Jiao","doi":"10.1093/plphys/kiag712","DOIUrl":"https://doi.org/10.1093/plphys/kiag712","url":null,"abstract":"Sorghum (Sorghum bicolor), a C4 grass adapted to hot semi-arid environments, depends on reinforced xylem vessels to maintain hydraulic conductance under high evaporative demand. During xylem differentiation, coordinated microtubule and actin dynamics guide secondary cell wall (SCW) deposition; however, whether actin-based motors regulate vascular architecture and hydraulic performance has remained unknown. Here, we identify HEAT-SENSITIVE 1 (HS1), a previously uncharacterized myosin VIII, as a central regulator of protoxylem integrity and water transport in sorghum. The hs1 mutant exhibited severe leaf scorching under field conditions, accompanied by pronounced protoxylem defects under controlled environments, including vessel collapse, reduced lumen area, and attenuated lignified SCWs. These structural abnormalities compromised longitudinal hydraulic conductance, diminished whole-plant water use, and rendered developing leaves unable to meet transpirational demand, resulting in transient water deficit and secondary tissue injury. HS1 encoded a grass-specific myosin VIII with a grass-specific N-terminal extension exhibiting high intrinsic disorder in N-terminal and lineage-specific substitutions in the motor domain. Single-cell transcriptome analyses positioned HS1 within differentiating protoxylem cells of developing leaves, revealing pronounced temporal and cell type specificity. Furthermore, bulk transcriptome profiling and quantitative lignin measurements indicated that HS1 is required for proper lignin deposition during protoxylem differentiation, linking an actin-based motor to wall reinforcement. Reduced nucleotide diversity at the HS1 locus further supports strong evolutionary constraint, suggesting an adaptive role in sorghum hydraulic resilience. Together, these findings establish HS1 as an actin-based motor controlling xylem architecture and hydraulic function in plants, a previously unrecognized component of grass hydraulic regulation not evident in Arabidopsis.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"7 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149021257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Trichoderma milRNA cross-species targeting chitin synthases genes is delivered by carbon nanomaterials to suppress Botrytis cinerea 利用碳纳米材料传递一种靶向几丁质合成酶基因的木霉milRNA以抑制灰葡萄孢

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-22 DOI: 10.1093/plphys/kiag715

Yaping Yin, Zhen Liu, Ming Xue, Xuewei Zhang, Raja Asad Ali Khan, Rui Wang, Jumei Hou, Tong Liu

{"title":"A Trichoderma milRNA cross-species targeting chitin synthases genes is delivered by carbon nanomaterials to suppress Botrytis cinerea","authors":"Yaping Yin, Zhen Liu, Ming Xue, Xuewei Zhang, Raja Asad Ali Khan, Rui Wang, Jumei Hou, Tong Liu","doi":"10.1093/plphys/kiag715","DOIUrl":"https://doi.org/10.1093/plphys/kiag715","url":null,"abstract":"Botrytis cinerea is a globally destructive plant pathogen that threatens major agricultural crops. Although biocontrol agents are widely used against this fungus, their inconsistent efficacy reflects a limited mechanistic understanding, hindering the development of effective disease management strategies. In this study, we investigated the in-depth biocontrol mechanism of Trichoderma breve against B. cinerea on tomato plants and various fruits. We identified a new microRNA Tri-milR29 from T. breve. Tri-milR29 is delivered into B. cinerea via extracellular vesicles, enabling cross-species RNA interference (RNAi). Once inside B. cinerea, Tri-milR29 targets the chitin synthase genes BcCHS7 and BcCHS6, thereby impairing fungal growth and reducing its pathogenicity. This cross-species miRNA phenomenon significantly enhanced the antifungal and disease-suppressing efficacy of T. breve against B. cinerea. Moreover, to optimize the antifungal and disease-control effects of Tri-milR29, we engineered polyethyleneimine-modified carbon dot nanocomposites (CPP) as nanocarriers using coconut water as a raw material. This green, biomass-based nanocarrier system improved the delivery and uptake of Tri-milR29 in B. cinerea. Pot experiments indicated that CPP-loaded Tri-milR29 improved disease control efficacy by 37.5%. A six-month field trial further confirmed that its field control rate reached 78.71%, which was close to the commercial fungicide procymidone (82.84%). Understanding the targets and mechanism of Tri-milR29, along with its enhanced performance via CPP facilitation, could support the broader application of dsRNA-CPP in other plant-pathogen systems, leading to reduced chemical inputs and encouraging safer, more environmentally-friendly alternatives.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"83 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149021256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inositol phosphates, pyrophosphates and the genes involved in their turnover in the streptophyte green alga Chara braunii 绿藻褐藻中肌醇磷酸、焦磷酸及其参与其转化的基因

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-09-22 DOI: 10.1093/plphys/kiag708

Daniel A Heß, Anuj Shukla, Henning Jacob Jessen, Wolfgang R Hess

{"title":"Inositol phosphates, pyrophosphates and the genes involved in their turnover in the streptophyte green alga Chara braunii","authors":"Daniel A Heß, Anuj Shukla, Henning Jacob Jessen, Wolfgang R Hess","doi":"10.1093/plphys/kiag708","DOIUrl":"https://doi.org/10.1093/plphys/kiag708","url":null,"abstract":"Inositol phosphates (InsPs) and inositol pyrophosphates (PP-InsPs) are conserved signalling molecules, but their evolutionary origin and diversification in the green lineage remain poorly understood. Here we investigated the InsP network in the streptophyte alga Chara braunii, a key lineage close to the origin of land plants. Using capillary electrophoresis–electrospray ionization mass spectrometry, we detected a broad spectrum of InsP and PP-InsP species from InsP3 to InsP8, including multiple positional isomers. Developmental profiling across dormant oospores, young thalli and mature thalli revealed extensive metabolic remodeling, with InsP6 as the dominant metabolite and distinct stage-dependent changes in lower InsPs and pyrophosphorylated species. Multiple PP-InsP5 and (PP)2-InsP4 isomers were identified, together with an unassigned additional InsP8-like signal, indicating further pathway complexity. Bioinformatic analyses identified candidate homologs of major InsP metabolic enzymes, supporting the presence of an enzymatic framework for InsP synthesis and turnover similar to land plants. Environmental perturbation revealed isomer-selective effects: prolonged light and dark phases strongly affected the accumulation of specific InsP5 and PP-InsP5 isomers, with 1-PP-InsP5 emerging as the most stimulus-responsive pyrophosphate species, whereas heat stress preferentially reduced 4-PP-InsP5. Together, these findings show that a structurally complex and environmentally responsive InsP network was already established in streptophyte algae before the emergence of land plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"101 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0