Plant Physiology最新文献_第10页

Packaging “vegetable oils”: Insights into plant lipid droplet proteins 包装 "植物油"：洞察植物脂滴蛋白

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-20 DOI: 10.1093/plphys/kiae533

Yingqi Cai, Patrick J Horn

{"title":"Packaging “vegetable oils”: Insights into plant lipid droplet proteins","authors":"Yingqi Cai, Patrick J Horn","doi":"10.1093/plphys/kiae533","DOIUrl":"https://doi.org/10.1093/plphys/kiae533","url":null,"abstract":"Plant neutral lipids, also known as “vegetable oils”, are synthesized within the endoplasmic reticulum (ER) membrane and packaged into subcellular compartments called lipid droplets (LDs) for stable storage in the cytoplasm. The biogenesis, modulation, and degradation of cytoplasmic LDs in plant cells are orchestrated by a variety of proteins localized to the ER, LDs, and peroxisomes. Recent studies of these LD-related proteins have greatly advanced our understanding of LDs not only as steady oil depots in seeds but also as dynamic cell organelles involved in numerous physiological processes in different tissues and developmental stages of plants. In the past 2 decades, technology advances in proteomics, transcriptomics, genome sequencing, cellular imaging and protein structural modeling have markedly expanded the inventory of LD-related proteins, provided unprecedented structural and functional insights into the protein machinery modulating LDs in plant cells, and shed new light on the functions of LDs in nonseed plant tissues as well as in unicellular algae. Here, we review critical advances in revealing new LD proteins in various plant tissues, point out structural and mechanistic insights into key proteins in LD biogenesis and dynamic modulation, and discuss future perspectives on bridging our knowledge gaps in plant LD biology.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"14 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CELL DIVISION CYCLE 5 controls floral transition by regulating flowering gene transcription and splicing in Arabidopsis. 细胞分裂周期 5 通过调控拟南芥开花基因的转录和剪接来控制花期转换。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-19 DOI: 10.1093/plphys/kiae616

Xin Xin, Linhan Ye, Tingting Zhai, Shu Wang, Yunjiao Pan, Ke Qu, Mengjie Gu, Yanjiao Wang, Jiedao Zhang, Xiang Li, Wei Yang, Shuxin Zhang

{"title":"CELL DIVISION CYCLE 5 controls floral transition by regulating flowering gene transcription and splicing in Arabidopsis.","authors":"Xin Xin, Linhan Ye, Tingting Zhai, Shu Wang, Yunjiao Pan, Ke Qu, Mengjie Gu, Yanjiao Wang, Jiedao Zhang, Xiang Li, Wei Yang, Shuxin Zhang","doi":"10.1093/plphys/kiae616","DOIUrl":"10.1093/plphys/kiae616","url":null,"abstract":"CELL DIVISION CYCLE 5 (CDC5) is a R2R3-type MYB transcription factor, serving as a key component of Modifier of snc1, 4 (MOS4)-associated complex (MAC)/NineTeen Complex (NTC), which is associated with plant immunity, RNA splicing, and miRNA biogenesis. In this study, we demonstrate that mutation of CDC5 accelerates flowering in Arabidopsis (Arabidopsis thaliana). CDC5 activates the expression of FLOWERING LOCUS C (FLC) by binding to and affecting the enrichment of RNA polymerase II on FLC chromatin. Moreover, genetic analysis confirmed that CDC5 regulates flowering in an FLC-dependent manner. Furthermore, we characterized the interaction of CDC5 with the RNA polymerase-associated factor 1 (Paf1) complex and confirmed that CDC5, as part of the spliceosome, mediates genome-wide alternative splicing, as revealed by RNA-Seq. CDC5 affected the splicing of flowering-associated genes such as FLC, SEF, and MAFs. Additionally, we also demonstrated that CDC5 contributes to the regulation of histone modification of FLC chromatin, which further promotes FLC expression. In summary, our results establish CDC5 as a key factor regulating flowering. This provides valuable insight for future research into plant flowering.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclic and pseudo-cyclic electron pathways play antagonistic roles during nitrogen deficiency in Chlamydomonas reinhardtii. 环状电子途径和假环状电子途径在莱茵衣藻缺氮过程中发挥拮抗作用

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-19 DOI: 10.1093/plphys/kiae617

Ousmane Dao, Adrien Burlacot, Felix Buchert, Marie Bertrand, Pascaline Auroy, Carolyne Stoffel, Sai Kiran Madireddi, Jacob Irby, Michael Hippler, Gilles Peltier, Yonghua Li-Beisson

{"title":"Cyclic and pseudo-cyclic electron pathways play antagonistic roles during nitrogen deficiency in Chlamydomonas reinhardtii.","authors":"Ousmane Dao, Adrien Burlacot, Felix Buchert, Marie Bertrand, Pascaline Auroy, Carolyne Stoffel, Sai Kiran Madireddi, Jacob Irby, Michael Hippler, Gilles Peltier, Yonghua Li-Beisson","doi":"10.1093/plphys/kiae617","DOIUrl":"10.1093/plphys/kiae617","url":null,"abstract":"Nitrogen (N) scarcity frequently constrains global biomass productivity. N deficiency halts cell division, downregulates photosynthetic electron transfer, and enhances carbon storage. However, the molecular mechanism downregulating photosynthesis during N deficiency and its relationship with carbon storage are not fully understood. Proton Gradient Regulator-like 1 (PGRL1) controlling cyclic electron flow (CEF) and Flavodiiron proteins (FLV) involved in pseudo-CEF (PCEF) are major players in the acclimation of photosynthesis. To determine the role of PGRL1 or FLV in photosynthesis under N deficiency, we measured photosynthetic electron transfer, oxygen gas exchange, and carbon storage in Chlamydomonas reinhardtii pgrl1 and flvB knockout mutants. Under N deficiency, pgrl1 maintained higher net photosynthesis and O2 photoreduction rates and higher levels of Cytochrome b6f and PSI compared to the control and flvB. The photosynthetic activity of flvB and pgrl1 flvB double mutants decreased in response to N deficiency, similar to the control strains. Furthermore, the preservation of photosynthetic activity in pgrl1 was accompanied by an increased accumulation of triacylglycerol in certain genetic backgrounds but not others, highlighting the importance of gene-environment interaction in determining traits such as oil content. Our results suggest that in the absence of PGRL1-controlled CEF, FLV-mediated PCEF maintains net photosynthesis at a high level and that CEF and PCEF play antagonistic roles during N deficiency. They further illustrate how a strain's nutrient status and genetic makeup can affect regulation of photosynthetic energy conversion in relation to carbon storage and provide additional strategies for improving lipid productivity in algae.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The E3 ubiquitin ligase COP1 and transcription factors HY5 and RHD6 integrate light signaling and root hair development. E3 泛素连接酶 COP1 以及转录因子 HY5 和 RHD6 整合了光信号和根毛的发育。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-19 DOI: 10.1093/plphys/kiae618

Tianen Zhang, Jingjuan Zhu, Yang Liu, Yanfei Pei, Yayue Pei, Zhenzhen Wei, Pengfei Miao, Jun Peng, Fuguang Li, Zhi Wang

{"title":"The E3 ubiquitin ligase COP1 and transcription factors HY5 and RHD6 integrate light signaling and root hair development.","authors":"Tianen Zhang, Jingjuan Zhu, Yang Liu, Yanfei Pei, Yayue Pei, Zhenzhen Wei, Pengfei Miao, Jun Peng, Fuguang Li, Zhi Wang","doi":"10.1093/plphys/kiae618","DOIUrl":"10.1093/plphys/kiae618","url":null,"abstract":"Light signaling plays a substantial role in regulating plant development, including the differentiation and elongation of single-celled tissue. However, the identity of the regulatory machine that affects light signaling on root hair cell (RHC) development remains unclear. Here, we investigated how darkness inhibits differentiation and elongation of RHC in Arabidopsis (Arabidopsis thaliana). We found that light promotes the growth and development of RHC. RNA-seq analysis showed that light signaling regulates the differentiation of RHC by promoting the expression of specific genes in the root epidermis associated with cell wall remodeling, JA, auxin, and ethylene signaling pathways. Together, these genes integrate light and phytohormone signals with root hair development. Our investigation also revealed that the core light signal factor ELONGATED HYPOCOTYL 5 (HY5) directly interacts with the key root hair development factor ROOT HAIR DEFECTIVE6 (RHD6), which promotes the transcription of RSL4. However, CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) repressed RHD6 function through the COP1-HY5 complex. Our genetic studies confirm associations between RHD6, HY5, and COP1, indicating that RHD6 largely depends on HY5 for root hair development. Ultimately, our work suggests a central COP1-HY5-RHD6 regulatory module that integrates light signaling and root hair development with several downstream pathways, offering perspectives to decipher single-celled root hair development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The wheat CC-NBS-LRR protein TaRGA3 confers resistance to stripe rust by suppressing Ascorbate peroxidase 6 activity. 小麦 CC-NBS-LRR 蛋白 TaRGA3 通过抑制抗坏血酸过氧化物酶 6 的活性来增强对条锈病的抗性。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-18 DOI: 10.1093/plphys/kiae603

Nannan Fang, Conghui Jia, Ruolin Chen, Jiarui An, Zhensheng Kang, Jie Liu

{"title":"The wheat CC-NBS-LRR protein TaRGA3 confers resistance to stripe rust by suppressing Ascorbate peroxidase 6 activity.","authors":"Nannan Fang, Conghui Jia, Ruolin Chen, Jiarui An, Zhensheng Kang, Jie Liu","doi":"10.1093/plphys/kiae603","DOIUrl":"10.1093/plphys/kiae603","url":null,"abstract":"Nucleotide-binding leucine-rich repeat (NLR) proteins are intracellular immune receptors that activate innate immune responses upon sensing pathogen attack. However, the molecular mechanisms by which NLR proteins initiate downstream signal transduction pathways to counteract pathogen invasion remain poorly understood. In this study, we identified the wheat (Triticum aestivum) NLR protein Resistance Gene Analogs3 (TaRGA3), which was significantly up-regulated during Puccinia striiformis f. sp. tritici (Pst) infection. TaRGA3 and its coiled-coil (CC) domain, localized to the cytoplasm and nucleus, can induce cell death in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) and overexpression suggested that TaRGA3 contributed to wheat resistance to stripe rust by facilitating reactive oxygen species (ROS) accumulation. Yeast two-hybrid, luciferase complementation imaging, and co-immunoprecipitation assays revealed that TaRGA3 interacted with wheat protein Ascorbate Peroxidase 6 (TaAPX6). Further analysis showed that TaAPX6 specifically targeted the CC domain of TaRGA3. The TaRGA3-TaAPX6 interplay led to reduced enzyme activity of TaAPX6. Notably, TaAPX6 negatively regulated wheat resistance to Pst by removing excessive ROS accompanying Pst-induced hypersensitive responses. Our findings reveal that TaRGA3 responding to Pst infection confers enhanced wheat resistance to stripe rust, possibly by suppressing TaAPX6-modulated ROS scavenging, and demonstrate that TaRGA3 can be used to engineer stripe rust resistance in wheat.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The E3 ligase TaE3V-B1 ubiquitinates proteins encoded by the vernalization gene TaVRN1 and regulates developmental processes in wheat. E3连接酶TaE3V-B1泛素化春化基因TaVRN1编码的蛋白，并调控小麦的发育过程。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-18 DOI: 10.1093/plphys/kiae606

Tian Li, Ragupathi Nagarajan, Shujuan Liu, Juan C Luzuriaga, Wenxuan Zhai, Shuanghe Cao, Haiyan Jia, Brett F Carver, Liuling Yan

{"title":"The E3 ligase TaE3V-B1 ubiquitinates proteins encoded by the vernalization gene TaVRN1 and regulates developmental processes in wheat.","authors":"Tian Li, Ragupathi Nagarajan, Shujuan Liu, Juan C Luzuriaga, Wenxuan Zhai, Shuanghe Cao, Haiyan Jia, Brett F Carver, Liuling Yan","doi":"10.1093/plphys/kiae606","DOIUrl":"10.1093/plphys/kiae606","url":null,"abstract":"In wheat (Triticum aestivum), early maturity is desired to avoid the hot and dry summer season, especially in view of climate change. Here, we report that TaE3V1, a C3H2C3 RING-type E3 ligase that interacts with TaVRN1, is associated with early development. Aside from its RING domain, TaE3V1 does not harbor any domains that are conserved in other RING-type or other E3 ligase proteins. TaE3V-B1b, encoded by the functional TaE3V1 allele, interacts with and ubiquitinates TaVRN1. In contrast, TaE3V-B1a, encoded by a natural nonfunctional TaE3V1 allele, neither interacts with TaVRN1 nor has E3 ligase activity. TaE3V-B1b activity decreases with plant age under warmer temperatures, but not under the low temperatures required for vernalization. We employed a gene editing method to simultaneously inactivate the three homoeologous TaE3V1 genes to validate their functions. Overall, our results suggest that the naturally mutated and edited TaE3V1 alleles can accelerate wheat development and aid adaptation to warming climates.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EARLY FLOWERING 3 alleles affect the temperature responsiveness of the circadian clock in Chinese cabbage 早花 3 等位基因影响大白菜昼夜节律钟的温度响应性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-15 DOI: 10.1093/plphys/kiae505

Shan Wang, Daling Feng, Yakun Zheng, Yin Lu, Kailin Shi, Rui Yang, Wei Ma, Na Li, Mengyang Liu, Yanhua Wang, Yiguo Hong, C Robertson McClung, Jianjun Zhao

{"title":"EARLY FLOWERING 3 alleles affect the temperature responsiveness of the circadian clock in Chinese cabbage","authors":"Shan Wang, Daling Feng, Yakun Zheng, Yin Lu, Kailin Shi, Rui Yang, Wei Ma, Na Li, Mengyang Liu, Yanhua Wang, Yiguo Hong, C Robertson McClung, Jianjun Zhao","doi":"10.1093/plphys/kiae505","DOIUrl":"https://doi.org/10.1093/plphys/kiae505","url":null,"abstract":"Temperature is an environmental cue that entrains the circadian clock, adapting it to local thermal and photoperiodic conditions that characterize different geographic regions. Circadian clock thermal adaptation in leafy vegetables such as Chinese cabbage (Brassica rapa ssp. pekinensis) is poorly understood but essential to sustain and increase vegetable production under changing climates. We investigated circadian rhythmicity in natural Chinese cabbage accessions grown at 14, 20, and 28 °C. The circadian period was significantly shorter at 20 °C than at either 14 or 28 °C, and the responses to increasing temperature and temperature compensation (Q10) were associated with population structure. Genome-wide association studies mapping identified variation responsible for temperature compensation as measured by Q10 value for temperature increase from 20 to 28 °C. Haplotype analysis indicated that B. rapa EARLY FLOWERING 3 H1 Allele (BrELF3H1) conferred a significantly higher Q10 value at 20 to 28 °C than BrELF3H2. Co-segregation analyses of an F2 population derived from a BrELF3H1 × BrELF3H2 cross revealed that variation among BrELF3 alleles determined variation in the circadian period of Chinese cabbage at 20 °C. However, their differential impact on circadian oscillation was attenuated at 28 °C. Transgenic complementation in Arabidopsis thaliana elf3-8 mutants validated the involvement of BrELF3 in the circadian clock response to thermal cues, with BrELF3H1 conferring a higher Q10 value than BrELF3 H2 at 20 to 28 °C. Thus, BrELF3 is critical to the circadian clock response to ambient temperature in Chinese cabbage. These findings have clear implications for breeding new varieties with enhanced resilience to extreme temperatures.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"75 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MID1-COMPLEMENTING ACTIVITY regulates cell proliferation and development via Ca2+ signaling in Marchantia polymorpha MID1-COMPLEMENTING ACTIVITY 通过 Ca2+ 信号调节马钱子的细胞增殖和发育

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-13 DOI: 10.1093/plphys/kiae613

Megumi Iwano, Noriyuki Suetsugu, Ryuichi Nishihama, Sakiko Ishida, Tomoaki Horie, Alex Costa, Tatsuya Katsuno, Midori Kimura, Kazuko Iida, Hidetoshi Iida, Takeharu Nagai, Takayuki Kohchi

{"title":"MID1-COMPLEMENTING ACTIVITY regulates cell proliferation and development via Ca2+ signaling in Marchantia polymorpha","authors":"Megumi Iwano, Noriyuki Suetsugu, Ryuichi Nishihama, Sakiko Ishida, Tomoaki Horie, Alex Costa, Tatsuya Katsuno, Midori Kimura, Kazuko Iida, Hidetoshi Iida, Takeharu Nagai, Takayuki Kohchi","doi":"10.1093/plphys/kiae613","DOIUrl":"https://doi.org/10.1093/plphys/kiae613","url":null,"abstract":"MID1-COMPLEMENTING ACTIVITY (MCA) is a land plant-specific, plasma membrane protein and Ca2+ signaling component that responds to exogenous mechanical stimuli, such as touch, gravity, and hypotonic-osmotic stress, in various plant species. MCA is essential for cell proliferation and differentiation during growth and development in rice (Oryza sativa) and maize (Zea mays). However, the mechanism by which MCA mediates cell proliferation and differentiation via Ca2+ signaling remains unknown. Here, we address this question using the liverwort Marchantia polymorpha. We show that the M. polymorpha MCA ortholog, MpMCA, is highly expressed in actively dividing regions, such as apical notches in the thalli and developing gametangiophores, and that MpMCA is a plasma membrane protein. In vivo Ca2+ imaging using a Ca2+ sensor (yellow cameleon) revealed that MpMCA is required for maintaining proper [Ca2+]cyt levels in the apical notch region, egg cells, and antheridium cells. Mpmca mutant plants showed severe cell proliferation and differentiation defects in the thalli, gametangiophores, and gametangia, resulting in abnormal development and unsuccessful fertilization. Furthermore, expression of the Arabidopsis MCA1 gene complemented most of the defects in the growth and development of the Mpmca mutant plants. Our findings indicate that MpMCA is an evolutionarily conserved Ca2+-signaling component that regulates cell proliferation and development across the life cycle of land plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor CaBBX10 promotes chlorophyll and carotenoid pigment accumulation in Capsicum annuum fruit 转录因子 CaBBX10 促进辣椒果实中叶绿素和类胡萝卜素色素的积累

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-13 DOI: 10.1093/plphys/kiae592

Jin Wang, Qingyun Shan, Qiaoling Yuan, Luzhao Pan, Meiqi Wang, Pei Zhao, Feng Yu, Li Dai, Lingling Xie, Zhongyi Wang, Xiongze Dai, Li Chen, Xuexiao Zou, Cheng Xiong, Fan Zhu, Feng Liu

{"title":"The transcription factor CaBBX10 promotes chlorophyll and carotenoid pigment accumulation in Capsicum annuum fruit","authors":"Jin Wang, Qingyun Shan, Qiaoling Yuan, Luzhao Pan, Meiqi Wang, Pei Zhao, Feng Yu, Li Dai, Lingling Xie, Zhongyi Wang, Xiongze Dai, Li Chen, Xuexiao Zou, Cheng Xiong, Fan Zhu, Feng Liu","doi":"10.1093/plphys/kiae592","DOIUrl":"https://doi.org/10.1093/plphys/kiae592","url":null,"abstract":"Chlorophylls and carotenoids are two pivotal photosynthetic pigments directly influencing the economic value of pepper (Capsicum annuum L.) fruits. However, the coordinated regulatory mechanisms governing the accumulation of both chlorophylls and carotenoids during pepper fruit development remain elusive. In this study, pepper B-box 10 (CaBBX10), a candidate hub transcription factor, was found to play dual roles in the early development of pepper fruit. CaBBX10 virus-induced gene silencing and overexpression experiments demonstrated that the encoded transcription factor promotes both chlorophyll and carotenoid accumulation in pepper fruit. Further comprehensive analyses showed that CaBBX10 directly binds to the promoter of magnesium chelatase subunit D subunit (CaCHLD) and phytoene synthase 1 (CaPSY1), thereby activating their expression in the chlorophyll and carotenoid biosynthesis pathways, respectively. Additionally, the photomorphogenic factor CaCOP1 was found to physically interact with CaBBX10 and lead to its degradation. Therefore, CaBBX10 may serve as a critical link connecting chlorophyll and carotenoid biosynthesis to light signaling. Altogether, our findings reveal a mechanism for the complex transcriptional regulation that simultaneously promotes chlorophyll and carotenoid accumulation in pepper fruit.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"20 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of the polyphenol oxidase gene MdPPO7 is modulated by MdWRKY3 to regulate browning in sliced apple fruit 多酚氧化酶基因 MdPPO7 的表达受 MdWRKY3 的调控，从而调节苹果切片果实的褐变过程

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-13 DOI: 10.1093/plphys/kiae614

Hui Zou, Chengzhu Li, Xiaoyu Wei, Qian Xiao, Xiaocheng Tian, Lingcheng Zhu, Baiquan Ma, Fengwang Ma, Mingjun Li

{"title":"Expression of the polyphenol oxidase gene MdPPO7 is modulated by MdWRKY3 to regulate browning in sliced apple fruit","authors":"Hui Zou, Chengzhu Li, Xiaoyu Wei, Qian Xiao, Xiaocheng Tian, Lingcheng Zhu, Baiquan Ma, Fengwang Ma, Mingjun Li","doi":"10.1093/plphys/kiae614","DOIUrl":"https://doi.org/10.1093/plphys/kiae614","url":null,"abstract":"Browning is a pervasive problem in horticultural products, substantially diminishing the appearance, flavor and nutritional value of fruit, including important fruits like apple (Malus × domestica Borkh.). In this study, we compared the physiological characteristics of the browning-resistant line ‘Rb-18’ with the susceptible variety ‘Fuji’ and found that the polyphenol oxidase (PPO) enzyme activity and phenolic content of Rb-18 were significantly lower than those in Fuji. In addition, the PPO enzyme in Fuji showed a stronger affinity for its substrate, catechol, compared to Rb-18. Through transcriptome and RT-qPCR analyses, MdPPO7 expression was identified as contributing to flesh browning after cutting. Subsequent fruit injection and stable genetic transformation of the MdPPO7 gene into apple fruit and calli determined that syringic acid, procyanidin, phloridzin, chlorogenic acid, gallic acid, catechin, and caffeic act as its catalytic substrates in the process involved in browning. Furthermore, luciferase reporter, yeast one-hybrid, β-glucuronidase reporter assays and chip-qPCR analysis demonstrated that a WRKY transcription factor(MdWRKY3) binds to the promoter region of polyphenol oxidase gene (MdPPO7) and positively regulates its expression to promote apple flesh browning. This study provides insights into the molecular regulatory mechanisms of fruit browning in fresh-cut apples and provides a theoretical basis for the generation of high-quality apple germplasm resources.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"41 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0