Plant Physiology最新文献_第10页

Substantial capacitance found in the roots of 2 contrasting conifer species. 在2种对比针叶树的根中发现了大量电容。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-30 DOI: 10.1093/plphys/kiaf116

Christopher McCarthy,Ibrahim Bourbia,Timothy Brodribb

{"title":"Substantial capacitance found in the roots of 2 contrasting conifer species.","authors":"Christopher McCarthy,Ibrahim Bourbia,Timothy Brodribb","doi":"10.1093/plphys/kiaf116","DOIUrl":"https://doi.org/10.1093/plphys/kiaf116","url":null,"abstract":"High rates of photosynthesis require abundant water delivered to the canopy to replace water lost to transpiration. In addition to water drawn immediately from the soil, stem capacitance has been identified as an additional water source, particularly during transient transpiration states. However, little information is available about the potential of roots to contribute to plant capacitance because methodological constraints have made it challenging to quantify root capacitance. In this study, we present a method to measure the water storage capacity of the root system and assess its contribution to daytime transpiration. We used an optical dendrometer to obtain in situ measurements of water potential and transpiration in 2 contrasting conifer species, Oyster Bay pine (Callitris rhomboidea) and Monterey pine (Pinus radiata), allowing us to quantify diurnal changes in plant water deficit. We employed a modified flow meter to gauge the rehydration kinetics of the below-ground and above-ground systems separately. We observed that root capacitance is a major supplier to the water demands during transient changes in transpiration for both species. Notably, the total below-ground capacitance exceeded the above-ground capacitance in C. rhomboidea, while the 2 capacitances were similar in P. radiata. Our findings highlight the importance of measuring and including below-ground capacitance in hydraulic models to accurately predict diurnal plant water status and stomatal behavior.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"65 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The regulatory landscape of β-caryophyllene biosynthesis in pak choi. 小白菜β-石竹烯生物合成的调控格局。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-30 DOI: 10.1093/plphys/kiaf123

Haibin Wang,Tiantian Han,Yibo Bai,Shuilin Yuan,Huanhuan Xu,Aimei Bai,Bilal A Rather,Tongkun Liu,Xilin Hou,Ying Li

{"title":"The regulatory landscape of β-caryophyllene biosynthesis in pak choi.","authors":"Haibin Wang,Tiantian Han,Yibo Bai,Shuilin Yuan,Huanhuan Xu,Aimei Bai,Bilal A Rather,Tongkun Liu,Xilin Hou,Ying Li","doi":"10.1093/plphys/kiaf123","DOIUrl":"https://doi.org/10.1093/plphys/kiaf123","url":null,"abstract":"β-Caryophyllene is a key volatile sesquiterpene involved in plant defense and contributes to the characteristic aroma of pak choi (Brassica campestris). This study aimed to elucidate the regulatory landscape of β-caryophyllene biosynthesis in pak choi to understand the genetic and molecular mechanisms controlling the production of this volatile sesquiterpene. Among 61 germplasm accessions of pak choi, β-caryophyllene was detected in only 11 accessions. Genetic analysis revealed that β-caryophyllene production is controlled by a single dominant gene. Fine mapping and gene sequencing identified the candidate gene B. campestris terpene synthases 21 (BcTPSa21), which encodes a β-caryophyllene synthase. Functional validation of BcTPSa21 through transient expression of BcTPSa21 in Nicotiana benthamiana leaves and enzyme activity assays in vitro confirmed its role in β-caryophyllene biosynthesis. A single nucleotide polymorphism (SNP) (C-T) in the promoter region of BcTPSa21 was found to affect the binding of the transcription factor BcMYC2, thereby influencing gene expression. Additionally, BcDIVARICATA (an R-R-type MYB TF BcDIV) was identified as a negative regulator of β-caryophyllene synthesis. The molecular experiments showed that abscisic acid participates in the biosynthesis of β-caryophyllene via the B. campestris pyrabactin resistance 1-like (BcPYL6)-BcDIVARICATA-BcMYC2 module. RNA-seq analysis suggested that under temperature stress, the transcription of BcTPSa21 and the biosynthesis of β-caryophyllene were the collective result of multilevel regulation. These findings provide comprehensive insights into the regulatory mechanisms governing β-caryophyllene biosynthesis in pak choi, identifying key factors and regulatory modules involved and offering a foundation for enhancing the flavor quality of pak choi through targeted genetic interventions.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"101 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The SAGA histone acetyltransferase complex functions in concert with RNA processing machinery to regulate wheat wax biosynthesis. SAGA组蛋白乙酰转移酶复合物与RNA加工机制协同作用，调节小麦蜡的生物合成。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-04-30 DOI: 10.1093/plphys/kiaf153

Xiaoyu Wang, Yixian Fu, Pengfei Zhi, Xiaofeng Liu, Pengkun Ge, Wenhui Zhang, Wanzhen Chen, Cheng Chang

{"title":"The SAGA histone acetyltransferase complex functions in concert with RNA processing machinery to regulate wheat wax biosynthesis.","authors":"Xiaoyu Wang, Yixian Fu, Pengfei Zhi, Xiaofeng Liu, Pengkun Ge, Wenhui Zhang, Wanzhen Chen, Cheng Chang","doi":"10.1093/plphys/kiaf153","DOIUrl":"https://doi.org/10.1093/plphys/kiaf153","url":null,"abstract":"Wax mixtures comprising very long-chain fatty acids and their derivatives represent the major cuticular components and protect plant tissues from environmental stresses. Uncovering the regulatory mechanisms underlying wax biosynthesis is essential for the genetic improvement of the agronomically important crop bread wheat (Triticum aestivum L.). Herein, partially redundant ECERIFERUM 3 (TaCER3) proteins were characterized as essential components of wheat wax biosynthetic machinery. Furthermore, we demonstrated that the wheat enoyl-CoA reductase promoter-binding MYB transcription factor 1 (TaEPBM1)could directly target TaCER3 genes and recruit components of the SAGA histone acetyltransferase complex to mediate histone acetylation, thereby stimulating TaCER3 transcription and potentiating wax biosynthesis. Wheat RNA processing machineries, including the RNA exosome, SUPERKILLER complex, cap-binding complex (CBC) components, TaSERRATE, and its partners, as well as elongator subunits, affected the accumulation of TaCER3 transcripts and controlled wax biosynthesis. Silencing of wheat CBC components, TaSERRATE, and elongator subunits resulted in the accumulation of TaCER3 transcripts and increased wax biosynthesis. Importantly, the activation of wheat wax biosynthesis in the absence of RNA processing factors was suppressed by silencing TaCER3 expression. These findings suggest that the SAGA histone acetyltransferase complex functions in concert with the RNA processing machinery to regulate wheat wax biosynthesis, probably via affecting TaCER3 genes.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"198 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytoplasmic Inheritance: The Transmission of Plastid and Mitochondrial Genomes Across Cells and Generations. 细胞质遗传：质体和线粒体基因组在细胞和代之间的传递。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-30 DOI: 10.1093/plphys/kiaf168

Kin Pan Chung

{"title":"Cytoplasmic Inheritance: The Transmission of Plastid and Mitochondrial Genomes Across Cells and Generations.","authors":"Kin Pan Chung","doi":"10.1093/plphys/kiaf168","DOIUrl":"https://doi.org/10.1093/plphys/kiaf168","url":null,"abstract":"In photosynthetic organisms, genetic material is stored in the nucleus and the two cytoplasmic organelles: plastids and mitochondria. While both the nuclear and cytoplasmic genomes are essential for survival, the inheritance of these genomes is subject to distinct laws. Cytoplasmic inheritance differs fundamentally from nuclear inheritance through two unique processes: vegetative segregation and uniparental inheritance. To illustrate the significance of these processes in shaping cytoplasmic inheritance, I will trace the journey of plastid and mitochondrial genomes, following their transmission from parents to progeny. The cellular and molecular mechanisms regulating their transmission along the path are explored. By providing a framework that encompasses the inheritance of both plastid and mitochondrial genomes across cells and generations, I aim to present a comprehensive overview of cytoplasmic inheritance and highlight the intricate interplay of cellular processes that determine inheritance patterns. I will conclude this review by summarizing recent breakthroughs in the field that have significantly advanced our understanding of cytoplasmic inheritance. This knowledge has paved the way for achieving the first instance of controlled cytoplasmic inheritance in plants, unlocking the potential to harness cytoplasmic genetics for crop improvement.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The thylakoid membrane remodeling protein VIPP1 forms bundled oligomers in tobacco chloroplasts. 类囊体膜重塑蛋白VIPP1在烟草叶绿体中形成束状低聚物。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-04-30 DOI: 10.1093/plphys/kiaf137

Sarah W Gachie, Alexandre Muhire, Di Li, Akihiro Kawamoto, Noriko Takeda-Kamiya, Yumi Goto, Mayuko Sato, Kiminori Toyooka, Ryo Yoshimura, Tsuneaki Takami, Lingang Zhang, Genji Kurisu, Toru Terachi, Wataru Sakamoto

{"title":"The thylakoid membrane remodeling protein VIPP1 forms bundled oligomers in tobacco chloroplasts.","authors":"Sarah W Gachie, Alexandre Muhire, Di Li, Akihiro Kawamoto, Noriko Takeda-Kamiya, Yumi Goto, Mayuko Sato, Kiminori Toyooka, Ryo Yoshimura, Tsuneaki Takami, Lingang Zhang, Genji Kurisu, Toru Terachi, Wataru Sakamoto","doi":"10.1093/plphys/kiaf137","DOIUrl":"https://doi.org/10.1093/plphys/kiaf137","url":null,"abstract":"The thylakoid membrane (TM) serves as the scaffold for oxygen-evolving photosynthesis, hosting the protein complexes responsible for the light reactions and ATP synthesis. Vesicle inducing protein in plastid 1 (VIPP1), a key protein in TM remodeling, has been recognized as essential for TM homeostasis. In vitro studies of cyanobacterial VIPP1 demonstrated its ability to form large homo-oligomers (2 MDa) manifesting as ring-like or filament-like assemblies associated with membranes. Similarly, VIPP1 in Chlamydomonas reinhardtii assembles into rods that encapsulate liposomes or into stacked spiral structures. However, the nature of VIPP1 assemblies in chloroplasts, particularly in Arabidopsis, remains uncharacterized. Here, we expressed Arabidopsis thaliana VIPP1 fused to GFP (AtVIPP1-GFP) in tobacco (Nicotiana tabacum) chloroplasts and performed transmission electron microscopy (TEM). A purified AtVIPP1-GFP fraction was enriched with long filamentous tubule-like structures. Detailed TEM observations of chloroplasts in fixed resin-embedded tissues identified VIPP1 assemblies in situ that appeared to colocalize with GFP fluorescence. Electron tomography demonstrated that the AtVIPP1 oligomers consisted of bundled filaments near membranes, some of which appeared connected to the TM or inner chloroplast envelope at their contact sites. The observed bundles were never detected in wild-type Arabidopsis but were observed in Arabidopsis vipp1 mutants expressing AtVIPP1-GFP. Taken together, we propose that the bundled filaments are the dominant AtVIPP1 oligomers that represent its static state in vivo.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"198 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ubiquitination of the PpMADS2 transcription factor controls linalool production during UV-B irradiation in detached peach fruit. PpMADS2转录因子的泛素化控制着UV-B辐照下桃果离体芳樟醇的产生。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-04-30 DOI: 10.1093/plphys/kiaf159

Chunyan Wei, Huizhen Yang, Bingbing Ye, Wei Wei, Wei Shan, Jianye Chen, Kunsong Chen, Xian Li, Zhiping Deng, Bo Zhang

{"title":"Ubiquitination of the PpMADS2 transcription factor controls linalool production during UV-B irradiation in detached peach fruit.","authors":"Chunyan Wei, Huizhen Yang, Bingbing Ye, Wei Wei, Wei Shan, Jianye Chen, Kunsong Chen, Xian Li, Zhiping Deng, Bo Zhang","doi":"10.1093/plphys/kiaf159","DOIUrl":"https://doi.org/10.1093/plphys/kiaf159","url":null,"abstract":"Plant secondary metabolites undergo changes in response to UV-B irradiation. Although UV-B irradiation reduces flavor-associated volatile compounds in detached peach (Prunus persica L. Batsch) fruit, the underlying regulatory mechanisms remain unclear. By integrating proteomic, transcriptomic, and metabolomic data from peach fruit following UV-B irradiation, we discovered that the detached fruit responds to UV-B by suppressing the biosynthesis of the flavor-related monoterpene linalool. We identified PpMADS2, a transcription factor that regulates linalool biosynthesis by activating terpene synthase 1 (PpTPS1) expression. PpMADS2 overexpression in peach and tomato fruits significantly increased linalool levels compared with the controls. Proteomic data and immunoblots revealed a decrease in PpMADS2 abundance following exposure to UV-B. Moreover, our results demonstrated that PpMADS2 interacts with the E3 ubiquitin ligase PpCOP1 both in vitro and in vivo. The UV-B-induced 26S-proteasome-mediated degradation of PpMADS2 is largely PpCOP1-dependent. Taken together, our findings demonstrate that linalool biosynthesis in detached peach fruit exposed to UV-B radiation is governed by the PpCOP1-PpMADS2-PpTPS1 module. This study enhances our understanding of the interplay between light signaling and fruit flavor quality. Multiomics approaches offer valuable resources for investigating the mechanisms underlying how light influences metabolism in fruit crops.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"198 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased root hydraulic traits in German winter wheat cultivars over 100 years of breeding. 100年来德国冬小麦品种根系水力性状的下降。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-04-30 DOI: 10.1093/plphys/kiaf166

Juan C Baca Cabrera, Jan Vanderborght, Yann Boursiac, Dominik Behrend, Thomas Gaiser, Thuy Huu Nguyen, Guillaume Lobet

{"title":"Decreased root hydraulic traits in German winter wheat cultivars over 100 years of breeding.","authors":"Juan C Baca Cabrera, Jan Vanderborght, Yann Boursiac, Dominik Behrend, Thomas Gaiser, Thuy Huu Nguyen, Guillaume Lobet","doi":"10.1093/plphys/kiaf166","DOIUrl":"https://doi.org/10.1093/plphys/kiaf166","url":null,"abstract":"Wheat (Triticum aestivum L.) plays a vital role in global food security, and understanding its root traits is essential for improving water uptake under varying environmental conditions. This study investigated how over a century of breeding has influenced root morphological and hydraulic properties in 6 German winter wheat cultivars released between 1895 and 2002. Field and hydroponic experiments were used to measure root diameter, root number, branching density, and whole root system hydraulic conductance (Krs). The results showed a significant decline in root axes number and Krs with release year, while root diameter remained stable across cultivars. Additionally, dynamic functional-structural modeling using the whole-plant model CPlantBox was employed to simulate Krs development with root system growth, revealing that older cultivars consistently had higher hydraulic conductance than modern ones. The combined approach of field phenotyping and modeling provided a comprehensive view of the changes in root traits arising from breeding. These findings suggest that breeding may have unintentionally favored cultivars with smaller root systems and more conservative water uptake strategies under the high-input, high-density conditions of modern agriculture. The results of this study may inform future breeding efforts aimed at optimizing wheat root systems, helping to develop cultivars with water uptake strategies better tailored to locally changing environmental conditions.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"198 1","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shared abscisic acid biosynthesis pathway across 600 million years of streptophyte evolution. 在6亿年的链藻进化中共享脱落酸生物合成途径。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-04-30 DOI: 10.1093/plphys/kiaf121

Matan Azar, Elisa Goldbecker, David Karpovsky, Michal Shpilman, Michal Breker, Jan de Vries, Assaf Mosquna

引用次数: 0

MYB80 and TEK: Dynamic duo regulating callose wall degradation and pollen exine development. MYB80和TEK：调节胼胝质壁降解和花粉外壁发育的动态组合。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-04-30 DOI: 10.1093/plphys/kiaf147

Nilesh D Gawande