Plant Physiology最新文献_第9页

Evolutionary trajectories and subfunctionalization of two key methyltransferase regulator subfamilies in plants. 植物中两个关键甲基转移酶调控亚家族的进化轨迹和亚功能化。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-07 DOI: 10.1093/plphys/kiaf191

Li-Yao Su,Zheng-Tai Liu,Xi-Liang Wang,Pei-Yan Chen,Hui Liu,Jin-Song Xiong,Ai-Sheng Xiong

{"title":"Evolutionary trajectories and subfunctionalization of two key methyltransferase regulator subfamilies in plants.","authors":"Li-Yao Su,Zheng-Tai Liu,Xi-Liang Wang,Pei-Yan Chen,Hui Liu,Jin-Song Xiong,Ai-Sheng Xiong","doi":"10.1093/plphys/kiaf191","DOIUrl":"https://doi.org/10.1093/plphys/kiaf191","url":null,"abstract":"DNA methylation, a conserved epigenetic mark in both plants and animals, plays a critical role in growth, development, and adaptability. This study explores the origin, evolution, and functional diversification of two key methyltransferase regulators, DNAJ domain-containing protein 1/2/3 (DNAJ1/2/3) and SU(VAR)3-9 HOMOLOG 1/3 (SUVH1/3), in plants. By analyzing genomic data from 21 algae and 86 land plants, we discovered that DNAJ1/2/3 originated within Magnoliopsida, while SUVH1/3 emerged in ferns and evolved through retrotransposition. Both protein families have undergone multiple duplication events and positive selection throughout plant evolution, resulting in their expansion and functional divergence. In dicotyledons, DNAJ1/2/3 diverged into three subclades, whereas SUVH1/3 underwent a common duplication event in its ancestral lineage, resulting in two subgroups. Structural domain analysis revealed that the evolution of PHD fingers in DNAJ1/2/3 and AT domains in SUVH1/3, under selective pressure, enhanced their interaction capabilities and contributed to the formation of complexes involved in DNA methylation and demethylation regulation. Expression profile analysis across various plant taxa demonstrated tissue-specific expression patterns, with higher expression levels observed in meristematic tissues and active cell regions. These findings elucidate the evolutionary patterns of DNAJ1/2/3 and SUVH1/3 and offer insights into their functional diversification in plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"47 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR858a-encoded peptide, miPEP858a, interacts with the miR858a promoter and requires the C-terminus for associated functions miR858a编码的肽miPEP858a与miR858a启动子相互作用，需要c端来实现相关功能

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-05 DOI: 10.1093/plphys/kiaf152

Himanshi Gautam, Ashish Sharma, Anwesha Anyatama, Hiteshwari Sinha, Prabodh Kumar Trivedi

{"title":"miR858a-encoded peptide, miPEP858a, interacts with the miR858a promoter and requires the C-terminus for associated functions","authors":"Himanshi Gautam, Ashish Sharma, Anwesha Anyatama, Hiteshwari Sinha, Prabodh Kumar Trivedi","doi":"10.1093/plphys/kiaf152","DOIUrl":"https://doi.org/10.1093/plphys/kiaf152","url":null,"abstract":"MicroRNAs (miRNAs) are key regulators of gene expression and typically processed from primary transcripts (pri-miRNAs). Recent discoveries highlight that certain pri-miRNAs also encode miRNA-encoded peptides (miPEPs), which influence miRNA function. However, the molecular mechanisms underlying miPEP activity, including the specific domains or essential amino acid residues required for their function, remain largely unexplored. In this study, we elucidated that the pri-miR858a-derived peptide, miPEP858a, directly interacts with the promoter of the MIR858 gene in Arabidopsis (Arabidopsis thaliana). Notably, the C-terminal region of miPEP858a, composed of 14 amino acid residues, is critical for its functionality. Through DNA–protein interaction assays, including yeast 1-hybrid, chromatin immunoprecipitation (ChIP-qPCR), electrophoretic mobility shift assay, and promoter–reporter analyses, we demonstrated that miPEP858a binds to a specific region within the MIR858 promoter. Exogenous application of a synthetic peptide corresponding to the C-terminal region of miPEP858a resulted in enhanced MIR858 expression, leading to phenotypic changes similar to those observed with the full-length miPEP858a. Moreover, the truncated C-terminal peptide was able to complement mutant plants lacking endogenous miPEP858a, emphasizing its role in regulating miR858a expression and downstream target genes involved in flavonoid biosynthesis and plant development. These findings suggest that the full-length miPEP858a may not be necessary for its biological function, with the C-terminal region being sufficient to modulate miRNA expression. This discovery reveals opportunities for identifying functional domains in other miPEPs, potentially reducing peptide synthesis costs, and offering a more efficient strategy for enhancing agronomic traits in crop plants without the need for complex biotechnological interventions.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"110 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How SynBio Can *Realistically* Impact Crop Improvement and Agriculture. 合成生物如何能够“现实地”影响作物改良和农业。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-05-03 DOI: 10.1093/plphys/kiaf179

Steven J Burgess, R Clay Wright, Karsten Temme, Catalin Voiniciuc, Andrew D Hanson

引用次数: 0

Characterization of the cyclic dipeptide cyclo(His-Pro) in Arabidopsis 拟南芥中环二肽环（His-Pro）的表征

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-03 DOI: 10.1093/plphys/kiaf174

Romina I Minen, Maria Dolores Camalle, Tyler Jeffrey Schwertfeger, Fatimah Abdulhakim, Hannah Reish, Leonardo Perez de Souza, Juan C Moreno, Anthony Schilmiller, Venkatesh P Thirumalaikumar, Pallavi Agarwal, Caroline F Plecki, Alisdair R Fernie, Heribert Hirt, Frank C Schroeder, Aleksandra Skirycz

{"title":"Characterization of the cyclic dipeptide cyclo(His-Pro) in Arabidopsis","authors":"Romina I Minen, Maria Dolores Camalle, Tyler Jeffrey Schwertfeger, Fatimah Abdulhakim, Hannah Reish, Leonardo Perez de Souza, Juan C Moreno, Anthony Schilmiller, Venkatesh P Thirumalaikumar, Pallavi Agarwal, Caroline F Plecki, Alisdair R Fernie, Heribert Hirt, Frank C Schroeder, Aleksandra Skirycz","doi":"10.1093/plphys/kiaf174","DOIUrl":"https://doi.org/10.1093/plphys/kiaf174","url":null,"abstract":"Diketopiperazines (DKPs) are chemically and functionally diverse cyclic dipeptides associated primarily with microbes. Few DKPs have been reported from plants and animals; the best characterized is cyclo(His-Pro), found in the mammalian central nervous system, where it arises from the proteolytic cleavage of a thyrotropin-releasing tripeptide hormone. Herein, we report the identification of cyclo(His-Pro) in Arabidopsis (Arabidopsis thaliana), where its levels increase upon abiotic stress conditions, including high salt, heat, and cold. To screen for potential protein targets, we used isothermal shift assays (iTSA), which examine changes in protein melting stability upon ligand binding. Among the identified proteins, we focused on the glycolytic enzyme, cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC1). Binding between the GAPC1 protein and cyclo(His-Pro) was validated using nano differential scanning fluorimetry (nanoDSF) and microscale thermophoresis (MST), and we could further demonstrate that cyclo(His-Pro) inhibits GAPC1 activity with an IC50 of approximately 200 μM. This inhibition was conserved in human GAPDH. Inhibition of glyceraldehyde-3-phosphate dehydrogenase activity has previously been reported to reroute carbon from glycolysis towards the pentose phosphate pathway. Accordingly, cyclo(His-Pro) supplementation augmented NADPH levels, increasing the NADPH/NADP+ ratio. Phenotypic screening revealed that plants supplemented with cyclo(His-Pro) were more tolerant to high salt stress, as manifested by higher biomass, which we show is dependent on GAPC1/2. Our work reports the identification and functional characterization of cyclo(His-Pro) as a modulator of glyceraldehyde-3-phosphate dehydrogenase in plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"16 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How to eat an idea - a roadmap for the translation and impact in plant biology. 如何吃一个想法-一个路线图的翻译和影响在植物生物学。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2025-05-03 DOI: 10.1093/plphys/kiaf178

Johnathan A Napier

引用次数: 0

Thermogenic tissues in lotus: Insights from multiscale imaging and calcium dynamics. 荷花的产热组织：来自多尺度成像和钙动力学的见解。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-02 DOI: 10.1093/plphys/kiaf173

Erin Cullen,Chong Teng

引用次数: 0

Protein homeostasis under heat stress: The role of BiP3, HsfA2, and chromatin remodeling in plants. 热胁迫下的蛋白质稳态：BiP3、HsfA2和植物染色质重塑的作用。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-02 DOI: 10.1093/plphys/kiaf172

Neeta Lohani,Prateek Jain

引用次数: 0

Harder Better Faster Stronger, and with less annotated data: ESGAN and plant sciences. 更硬，更好，更快，更强，注释数据更少：ESGAN和植物科学。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-02 DOI: 10.1093/plphys/kiaf171

Thomas Depaepe

引用次数: 0

Auxin! here you go again: Spatiotemporal dynamic regulation of auxin promotes proper nodule formation in Medicago truncatula. 生长素!又来了：生长素的时空动态调节促进了苜蓿根瘤的形成。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-02 DOI: 10.1093/plphys/kiaf177

Gunjan Sharma,Héctor H Torres-Martínez

引用次数: 0

Acceptance of Crop Biotechnology Requires a Change in Communication Strategy 接受作物生物技术需要改变传播策略

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-02 DOI: 10.1093/plphys/kiaf167

Kevin M Folta

{"title":"Acceptance of Crop Biotechnology Requires a Change in Communication Strategy","authors":"Kevin M Folta","doi":"10.1093/plphys/kiaf167","DOIUrl":"https://doi.org/10.1093/plphys/kiaf167","url":null,"abstract":"Ever since the first transgenic plant emerged from a green clump of callus, grant proposals were erected upon grand ideas, visions of crop genetic engineering innovations positively impacting people and the planet. But how many of these actually came to fruition? More than three decades of journal articles articulate the discoveries of gene-trait connections and how they may be implemented to improve profits for farmers, products for consumers, environmental stewardship, and the plight of the food insecure. The shelves and autoclaves of academic, government and industry laboratories speak stories of innovation unrealized. Today’s latest gene editing technologies stand to speed innovation with greater precision with less perception of risk-- but will the next wave of crop solutions created via transgenesis or site-directed nucleases also fail to reach the field? Their deployment is not limited by safety or utility. Instead, they are hindered by a lack of social license to implement technology, driven by understandable concerns, many not based in reality, and some stoked by well-constructed disinformation campaigns. The solution is public engagement, yet scientists engage at low frequency, and fail to connect in effective ways when they do engage. The goal of this article is to illuminate the agricultural biotechnology communication chasm, how it happened, its effects, and implementable solutions. Scientists need to understand the how information flows, the social guardrails that impede information flow, and ways to bypass psychological barriers to deliver trusted information. Rapid deployment of next generation plant biology solutions is dependent on scientists retooling their communication strategies, and then becoming part of the social conversation.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"9 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0