Plant Physiology最新文献_第9页

Examining photosynthetic induction variation among historical cotton cultivars through time-integrated limitation analyses 利用时间积分限制分析研究历史棉花品种间光合诱导变异

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-24 DOI: 10.1093/plphys/kiaf157

Yonghui Pan, Margaret M Barbour, Tao Liu, Sen Rao, Xin Song

{"title":"Examining photosynthetic induction variation among historical cotton cultivars through time-integrated limitation analyses","authors":"Yonghui Pan, Margaret M Barbour, Tao Liu, Sen Rao, Xin Song","doi":"10.1093/plphys/kiaf157","DOIUrl":"https://doi.org/10.1093/plphys/kiaf157","url":null,"abstract":"A thorough understanding of the biochemical, stomatal, and mesophyll components that limit photosynthetic induction is crucial for targeted improvement of crop productivity. However, compared with biochemical activation and stomatal conductance (gs), mesophyll conductance (gm) remains underexplored in induction studies. The fluorescence method (the variable J method) is a valid and widely accessible tool for gm measurement under steady-state conditions. Here, we experimentally validated the applicability of the fluorescence method under non-steady-state conditions, demonstrating comparable induction kinetics of gm with the well-established carbon isotope method. Building on this validation, we combined the fluorescence method with gas exchange measurements to comprehensively examine the induction kinetics of photosynthetic rate (A) and its associated components in a set of historical cotton (Gossypium hirsutum L.) cultivars. Our results showed no significant effect of the year of cultivar release on A during induction, suggesting that dynamic photosynthesis has not benefited from past selection efforts in cotton. Nonetheless, significant among-cultivar variations were observed in all measured induction traits, hinting at breeding opportunities for leveraging dynamic photosynthetic variation to boost crop productivity. Through induction-period-integrated limitation analysis, we further identified gs as the single most important limiter of photosynthetic induction across all cotton cultivars. Moreover, the analysis also demonstrated that accurately accounting for gm kinetics is essential for the unbiased acquisition of mechanistic insights into non-steady-state photosynthetic physiology. We recommend that future induction studies incorporate gm measurements whenever possible to strengthen the knowledge base necessary for genetically enhancing dynamic carbon gain and crop yield in the field.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"91 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breaking the barrier of human-annotated training data for machine learning-aided plant research using aerial imagery 打破人工标注训练数据的障碍，利用航空图像进行机器学习辅助植物研究

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-23 DOI: 10.1093/plphys/kiaf132

Sebastian Varela, Xuying Zheng, Joyce Njuguna, Erik Sacks, Dylan Allen, Jeremy Ruhter, Andrew D B Leakey

{"title":"Breaking the barrier of human-annotated training data for machine learning-aided plant research using aerial imagery","authors":"Sebastian Varela, Xuying Zheng, Joyce Njuguna, Erik Sacks, Dylan Allen, Jeremy Ruhter, Andrew D B Leakey","doi":"10.1093/plphys/kiaf132","DOIUrl":"https://doi.org/10.1093/plphys/kiaf132","url":null,"abstract":"Machine learning (ML) can accelerate biological research. However, the adoption of such tools to facilitate phenotyping based on sensor data has been limited by (i) the need for a large amount of human-annotated training data for each context in which the tool is used and (ii) phenotypes varying across contexts defined in terms of genetics and environment. This is a major bottleneck because acquiring training data is generally costly and time-consuming. This study demonstrates how a ML approach can address these challenges by minimizing the amount of human supervision needed for tool building. A case study was performed to compare ML approaches that examine images collected by an uncrewed aerial vehicle to determine the presence/absence of panicles (i.e. “heading”) across thousands of field plots containing genetically diverse breeding populations of 2 Miscanthus species. Automated analysis of aerial imagery enabled the identification of heading approximately 9 times faster than in-field visual inspection by humans. Leveraging an Efficiently Supervised Generative Adversarial Network (ESGAN) learning strategy reduced the requirement for human-annotated data by 1 to 2 orders of magnitude compared to traditional, fully supervised learning approaches. The ESGAN model learned the salient features of the data set by using thousands of unlabeled images to inform the discriminative ability of a classifier so that it required minimal human-labeled training data. This method can accelerate the phenotyping of heading date as a measure of flowering time in Miscanthus across diverse contexts (e.g. in multistate trials) and opens avenues to promote the broad adoption of ML tools.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"31 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Crassulacean acid metabolism at the protein level in Kalanchoë laxiflora Kalanchoë草属植物在蛋白质水平上对天冬氨酸代谢的调控

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-22 DOI: 10.1093/plphys/kiaf095

Katharina Schiller, Saskia Janshoff, Sanja Zenker, Prisca Viehöver, James Hartwell, Jürgen Eirich, Iris Finkemeier, Andrea Bräutigam

{"title":"Regulation of Crassulacean acid metabolism at the protein level in Kalanchoë laxiflora","authors":"Katharina Schiller, Saskia Janshoff, Sanja Zenker, Prisca Viehöver, James Hartwell, Jürgen Eirich, Iris Finkemeier, Andrea Bräutigam","doi":"10.1093/plphys/kiaf095","DOIUrl":"https://doi.org/10.1093/plphys/kiaf095","url":null,"abstract":"Crassulacean acid metabolism (CAM) is an adaptation to environments where water availability is seasonal or extremely low. It serves to ensure plant survival and/or maintain productivity in these adverse environments. CAM has repeatedly evolved in many plant lineages, although it requires a large and complex set of enzymes, transporters, and regulatory processes to control metabolite flux and pools. To test the potential levels at which CAM is regulated, we analyzed the CAM plant Kalanchoë laxiflora and compared with the genomes and transcriptomes of other CAM plants across a wide phylogenetic range. We show that CAM-associated transcripts and proteins did not exhibit a binary on/off pattern in abundance between day and night in K. laxiflora. Instead, K. laxiflora and many CAM plants displayed shared amino acid changes among proteins compared to C3 plants, especially in starch metabolism. Phosphoproteomics identified differential phosphorylation in K. laxiflora proteins between day and night. Taken together, our results demonstrate that CAM photosynthesis is regulated at both the transcript and protein levels.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"96 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Essential Yet Dispensable: The Role of CINNAMATE 4-HYDROXYLASE in Rice Cell Wall Lignification 必要但不可缺少：肉桂酸4-羟化酶在水稻细胞壁木质化中的作用

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-22 DOI: 10.1093/plphys/kiaf164

Supatmi Supatmi, Lydia Pui Ying Lam, Senri Yamamoto, Osama A Afifi, Pingping Ji, Yuriko Osakabe, Keishi Osakabe, Toshiaki Umezawa, Yuki Tobimatsu

{"title":"Essential Yet Dispensable: The Role of CINNAMATE 4-HYDROXYLASE in Rice Cell Wall Lignification","authors":"Supatmi Supatmi, Lydia Pui Ying Lam, Senri Yamamoto, Osama A Afifi, Pingping Ji, Yuriko Osakabe, Keishi Osakabe, Toshiaki Umezawa, Yuki Tobimatsu","doi":"10.1093/plphys/kiaf164","DOIUrl":"https://doi.org/10.1093/plphys/kiaf164","url":null,"abstract":"A comprehensive understanding of the intricate lignin biosynthesis in grasses could contribute to enhancing our ability to utilize grass biomass. CINNAMATE 4-HYDROXYLASE (C4H), in conjunction with PHENYLALANINE AMMONIA-LYASE (PAL), initiates the entry of phenylalanine into the cinnamate/monolignol pathway, leading to the production of diverse phenylpropanoids, including lignin monomers. Despite extensive research on C4H in eudicots, genetic studies of C4H in grasses remain considerably limited. Notably, the role of C4H in the presence of PHENYLALANINE/TYROSINE AMMONIA-LYASE (PTAL), a grass-specific ammonia-lyase that can bypass the conserved PAL-C4H pathway by recruiting tyrosine into the cinnamate/monolignol pathway, remains unclear. To address this gap, a set of genome-edited rice (Oryza sativa) mutants harboring knockout mutations in rice C4H genes were generated and subjected to the analysis of growth phenotype and cell wall chemotype, alongside isotopic feeding and chemical inhibitor assays to test the contributions of the PAL-C4H and PTAL pathways. The phenotype and chemotype characterizations of C4H-knockout rice mutants demonstrated that class I (OsC4H1) and class II (OsC4H2a and OsC4H2b) C4Hs cooperatively contribute to lignin biosynthesis in rice. Nevertheless, the effects of C4H deficiency on plant development and lignin formation in rice appeared to be markedly less prominent compared to those reported in eudicots. The 13C-labeled phenylalanine and tyrosine feeding experiments demonstrated that even with the phenylalanine-derived PAL-C4H pathway completely blocked, C4H-knockout rice could still produce substantial levels of lignin and maintain sound cell walls by utilizing the tyrosine-derived PTAL pathway. Overall, this study demonstrates the essential but dispensable role of C4H in grass cell wall lignification.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"36 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BTB/POZ-MATH proteins regulate Arabidopsis seedling development by promoting auxin-independent degradation of the Aux/IAA protein IAA10 BTB/POZ-MATH蛋白通过促进Aux/IAA蛋白IAA10的生长素非依赖性降解来调节拟南芥幼苗发育

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-21 DOI: 10.1093/plphys/kiaf155

Zhaonan Ban, Yueh-Ju Hou, Ellyse Ku, YingLin Zhu, Yun Hu, Natalie Karadanaian, Yunde Zhao, Mark Estelle

{"title":"BTB/POZ-MATH proteins regulate Arabidopsis seedling development by promoting auxin-independent degradation of the Aux/IAA protein IAA10","authors":"Zhaonan Ban, Yueh-Ju Hou, Ellyse Ku, YingLin Zhu, Yun Hu, Natalie Karadanaian, Yunde Zhao, Mark Estelle","doi":"10.1093/plphys/kiaf155","DOIUrl":"https://doi.org/10.1093/plphys/kiaf155","url":null,"abstract":"After germination, seedlings undergo etiolated development (skotomorphogenesis), enabling them to grow toward the soil surface. In Arabidopsis (Arabidopsis thaliana), etiolated seedlings exhibit rapid hypocotyl elongation, apical hook formation, and closed cotyledons to protect the meristem. In this study, we found that high-order mutants in the BPM (BTB/POZ-MATH) gene family displayed defects in seedling development, characterized by a shorter hypocotyl, early apical hook opening, and opened cotyledons in the dark. BPM1, BPM2, BPM4, and BPM5 exhibited distinct expression patterns and subcellular localization in etiolated seedlings. In a hypocotyl segment assay, the bpm mutants showed defects in auxin response, indicating impaired auxin signaling in the hypocotyl. Expression of the auxin reporter DR5:GFP was also altered in the bpm1,4,5 mutant in various tissues compared to the wild type. Furthermore, yeast two-hybrid, bimolecular fluorescence complementation (BiFC), and co-immunoprecipitation (Co-IP) assay analyses showed that BPM1 interacts with IAA10. Experiments in protoplasts indicated that BPM1 promotes IAA10 ubiquitylation and degradation, which was supported by greater IAA10 protein accumulation in the bpm1,4,5 mutant background. In addition, IAA10 over-expression resulted in phenotypes similar to those of the bpm mutants, indicating that the BPMs may target the Aux/IAA proteins for ubiquitylation and degradation. Overall, our findings shed light on the key roles of the BPMs in auxin signaling during seedling development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"15 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cistanthe longiscapa exhibits ecophysiological and molecular adaptations to the arid environments of the Atacama Desert Cistanthe longiscapa表现出对阿塔卡马沙漠干旱环境的生态生理和分子适应性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-16 DOI: 10.1093/plphys/kiaf068

Paulina Ossa, Adrián A Moreno, Daniela Orellana, Mónica Toro, Tomás Carrasco-Valenzuela, Anibal Riveros, Claudio Meneses, Ricardo Nilo-Poyanco, Ariel Orellana

{"title":"Cistanthe longiscapa exhibits ecophysiological and molecular adaptations to the arid environments of the Atacama Desert","authors":"Paulina Ossa, Adrián A Moreno, Daniela Orellana, Mónica Toro, Tomás Carrasco-Valenzuela, Anibal Riveros, Claudio Meneses, Ricardo Nilo-Poyanco, Ariel Orellana","doi":"10.1093/plphys/kiaf068","DOIUrl":"https://doi.org/10.1093/plphys/kiaf068","url":null,"abstract":"Understanding how plants survive extreme conditions is essential to breeding resilient crops. Cistanthe longiscapa, which flourishes in the Atacama Desert, provides a rare glimpse into plant resilience. To uncover the genetic basis of its stress tolerance, we investigated the ecophysiological and transcriptomic responses of C. longiscapa from 3 sites with low but different precipitation levels. Ecophysiological analyses were performed on samples collected in the field at dusk and dawn, which are crucial stages in crassulacean acid metabolism (CAM), a water-efficient type of photosynthesis. Additional transcriptomic analysis allowed us to evaluate CAM intensity in C. longiscapa and identify changes in the molecular signature of these plants. Our results show that C. longiscapa displays considerable ecophysiological trait response variation across the 3 sites, including variations in markers such as nocturnal acid accumulation, isotopic carbon ratio, and succulence, among others. Analysis of gene expression patterns revealed differences among plants exhibiting varying intensities of CAM photosynthesis and identified key molecular signatures associated with their ecological strategies. Additionally, genes related to stress responses, plastid activities, and circadian rhythm show contrasting expression levels between strong and weak CAM plants, and this expression profile is shared with other CAM plants under stress. Our findings demonstrate that C. longiscapa is a valuable resource for identifying genes involved in the transition between different CAM intensities. This may lead to the discovery of genes that enhance plant tolerance to stressful environments.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"74 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143837041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subcellular plant carbohydrate metabolism under elevated temperature 高温条件下的植物碳水化合物亚细胞代谢

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-16 DOI: 10.1093/plphys/kiaf117

Charlotte Seydel, Martin Heß, Laura Schröder, Andreas Klingl, Thomas Nägele

{"title":"Subcellular plant carbohydrate metabolism under elevated temperature","authors":"Charlotte Seydel, Martin Heß, Laura Schröder, Andreas Klingl, Thomas Nägele","doi":"10.1093/plphys/kiaf117","DOIUrl":"https://doi.org/10.1093/plphys/kiaf117","url":null,"abstract":"In many plant species, exposure to a changing environmental temperature regime induces an acclimation response that ultimately increases thermotolerance. Under elevated temperature, membrane systems undergo remodeling to counteract destabilizing thermodynamic effects. Elevated temperature also affects photosynthesis and carbohydrate metabolism due to altered protein functions, enzyme activities, and transport across membrane systems. Here, a combination of electrolyte leakage assays and chlorophyll fluorescence measurements was applied to quantify heat tolerance before and after heat acclimation in Arabidopsis thaliana under different temperature regimes. Subcellular carbohydrate concentrations were determined through non-aqueous fractionation and 3D reconstruction of mesophyll cells and subcellular compartments using serial block-face scanning electron microscopy. Across temperature regimes between 32 °C and 38 °C, seven days of heat acclimation at 34 °C most efficiently increased tissue heat tolerance. Under such conditions, cytosolic sucrose concentrations were stabilized by a shift in sucrose cleavage rates into the vacuolar compartment, while invertase-driven cytosolic sucrose cleavage was efficiently quenched by fructose and glucose acting as competitive and non-competitive inhibitors, respectively. Finally, this study provides strong evidence for a sucrose concentration gradient from the cytosol into the vacuole, which might directly affect the physiological role and direction of sugar transport across cellular membrane systems.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"18 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor FveMYB117a controls leaf morphology by coordinating auxin and cytokinin signals in woodland strawberry 转录因子FveMYB117a通过协调生长素和细胞分裂素信号控制林地草莓叶片形态

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-15 DOI: 10.1093/plphys/kiaf156

Yafan Han, Rui Lu, Danni Yan, Zhongchi Liu, Xi Luo, Chunying Kang

{"title":"The transcription factor FveMYB117a controls leaf morphology by coordinating auxin and cytokinin signals in woodland strawberry","authors":"Yafan Han, Rui Lu, Danni Yan, Zhongchi Liu, Xi Luo, Chunying Kang","doi":"10.1093/plphys/kiaf156","DOIUrl":"https://doi.org/10.1093/plphys/kiaf156","url":null,"abstract":"Leaf morphology affects physiological activities and contributes to environment adaptation. The phytohormones auxin and cytokinin both regulate leaf shape, but how they act together to specify leaf complexity is not fully understood. In woodland strawberry (Fragaria vesca), the wild type develops trifoliate leaves, whereas myb117a mutants produce one to five leaflets with fewer serrations. Transcriptome analysis revealed that the auxin biosynthesis gene YUCCA4 (FveYUC4), the cytokinin biosynthesis gene ISOPENTENYL TRANSFERASE2 (FveIPT2), the cytokinin degradation gene CYTOKININ OXIDASE/DEHYDROGENASE1 (FveCKX1), and the transcription factor gene CUP-SHAPED COTYLEDON2 (FveCUC2a) are altered in the myb117a mutant. Accordingly, the myb117a leaves contain lower auxin levels and higher cytokinin levels compared to wild type. Moreover, treatment with the auxin transport inhibitor NPA produced simple leaves with smooth margins, whereas exogenous cytokinin application resulted in a higher percentage of four to five leaflets in myb117a. Several lines of evidence showed that FveMYB117a can directly bind to the promoters of FveYUC4 and FveCUC2a and influence their expression. Both myb117a yuc4 and myb117a cuc2a double mutants had fewer leaflets with greatly reduced or no serrations compared to myb117a, suggesting that these genes function in the same pathway. Overall, our results indicate that FveMYB117a is a transcription factor that coordinates auxin and cytokinin homeostasis in young leaves, thereby contributing to robust leaf morphogenesis in strawberry.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"8 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The SAGA histone acetyltransferase complex functions in concert with RNA processing machinery to regulate wheat wax biosynthesis SAGA组蛋白乙酰转移酶复合物与RNA加工机制协同作用，调节小麦蜡的生物合成

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-12 DOI: 10.1093/plphys/kiaf153

Xiaoyu Wang, Yixian Fu, Pengfei Zhi, Xiaofeng Liu, Pengkun Ge, Wenhui Zhang, Wanzhen Chen, Cheng Chang

{"title":"The SAGA histone acetyltransferase complex functions in concert with RNA processing machinery to regulate wheat wax biosynthesis","authors":"Xiaoyu Wang, Yixian Fu, Pengfei Zhi, Xiaofeng Liu, Pengkun Ge, Wenhui Zhang, Wanzhen Chen, Cheng Chang","doi":"10.1093/plphys/kiaf153","DOIUrl":"https://doi.org/10.1093/plphys/kiaf153","url":null,"abstract":"Wax mixtures comprising very long-chain fatty acids and their derivatives represent the major cuticular components and protect plant tissues from environmental stresses. Uncovering the regulatory mechanisms underlying wax biosynthesis is essential for the genetic improvement of the agronomically important crop bread wheat (Triticum aestivum L.). Herein, partially redundant ECERIFERUM 3 (TaCER3) proteins were characterized as essential components of wheat wax biosynthetic machinery. Furthermore, we demonstrated that the wheat Enoyl-CoA Reductase promoter-binding MYB transcription factor 1 (TaEPBM1)could directly target TaCER3 genes and recruit components of the SAGA histone acetyltransferase complex to mediate histone acetylation, thereby stimulating TaCER3 transcription and potentiating wax biosynthesis. Wheat RNA processing machineries, including the RNA exosome, SUPERKILLER (SKI) complex, cap-binding complex (CBC) components, TaSERRATE and its partners, as well as elongator subunits, affected the accumulation of TaCER3 transcripts and controlled wax biosynthesis. Silencing of wheat CBC components, TaSERRATE, and elongator subunits resulted in the accumulation of TaCER3 transcripts and increased wax biosynthesis. Importantly, the activation of wheat wax biosynthesis in the absence of RNA processing factors was suppressed by silencing TaCER3 expression. These findings suggest that the SAGA histone acetyltransferase complex functions in concert with the RNA processing machinery to regulate wheat wax biosynthesis, probably via affecting TaCER3 genes.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"110 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water stress enhances triacylglycerol accumulation via different mechanisms in wild-type and transgenic high-leaf oil tobacco 水分胁迫通过不同机制促进野生型和转基因高叶油烟甘油三酯的积累

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-04-12 DOI: 10.1093/plphys/kiaf151

Jing Zhang, Ingrid Venables, Damien L Callahan, Alexander B Zwart, John Passioura, Qing Liu, Xue-Rong Zhou, Thomas Vanhercke, Gonzalo M Estavillo

{"title":"Water stress enhances triacylglycerol accumulation via different mechanisms in wild-type and transgenic high-leaf oil tobacco","authors":"Jing Zhang, Ingrid Venables, Damien L Callahan, Alexander B Zwart, John Passioura, Qing Liu, Xue-Rong Zhou, Thomas Vanhercke, Gonzalo M Estavillo","doi":"10.1093/plphys/kiaf151","DOIUrl":"https://doi.org/10.1093/plphys/kiaf151","url":null,"abstract":"Metabolically engineered high-leaf oil plants have been developed to meet the increasing demand for plant oils. Oil production of these plants under controlled conditions is promising; however, their performance under field-like conditions with abiotic stresses remains uncertain. In this study, wild-type (WT) and high-leaf oil (HLO) transgenic tobacco (Nicotiana tabacum) plants were exposed to moderate and sustained water stress to mimic field conditions. The effects of water stress on biomass and lipid accumulation were investigated at the physiological, biochemical, and transcriptional levels. The presence of transgenes increased leaf triacylglycerol (TAG) levels in HLO plants by upregulating endogenous genes involved in lipid biosynthesis at the expense of biomass reduction, altered leaf lipid content and profile, and a decrease in unsaturation levels of membrane lipids compared to WT plants. Moreover, the biomass penalty in HLO plants could reduce canopy transpiration, contributing to their better performance under water-limited environments. Furthermore, WT and HLO plants exhibited enhanced TAG accumulation under water stress but via different mechanisms. In WT plants, water stress induced lipid remodeling, upregulated genes encoding phosphatidic acid phosphatase (PAP), diacylglycerol o-acyltransferase (DGAT2), and lipid droplet-associated proteins (LDAP1), but downregulated genes encoding Gly-Asp-Ser-Leu (GDSL) lipases. In contrast, HLO plants showed increased TAG accumulation primarily through upregulation of OLEOSINS and downregulation of GDSLs under water stress. In conclusion, moderate water stress promoted oil production in HLO plants, demonstrating the robustness of HLO technology for sustainable oil production in the field under water deficit conditions which may be more prevalent in the future due to climate change.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"5 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0