Plant Physiology最新文献_第9页

The H2S-responsive transcription factor ERF.D3 regulates tomato abscisic acid metabolism, leaf senescence, and fruit ripening. H2S反应性转录因子ERF.D3调控番茄赤霉酸代谢、叶片衰老和果实成熟。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-10-21 DOI: 10.1093/plphys/kiae560

Kangdi Hu,Meihui Geng,Lin Ma,Gaifang Yao,Min Zhang,Hua Zhang

{"title":"The H2S-responsive transcription factor ERF.D3 regulates tomato abscisic acid metabolism, leaf senescence, and fruit ripening.","authors":"Kangdi Hu,Meihui Geng,Lin Ma,Gaifang Yao,Min Zhang,Hua Zhang","doi":"10.1093/plphys/kiae560","DOIUrl":"https://doi.org/10.1093/plphys/kiae560","url":null,"abstract":"Hydrogen sulfide (H2S) is a signaling molecule that regulates plant senescence. In this study, we found that H2S delays dark-induced senescence in tomato (Solanum lycopersicum) leaves. Transcriptome and RT-qPCR analyses revealed an Ethylene Response Factor ERF.D3 is quickly induced by H2S. H2S also persulfidated ERF.D3 at amino acid residues C115 and C118. CRISPR/Cas9-mediated gene editing and gene overexpression analyses showed that ERF.D3 negatively regulates leaf senescence and fruit ripening. Abscisic acid (ABA) levels were reduced by ERF.D3 overexpression, suggesting ERF.D3 might regulate ABA metabolism. Additionally, the abscisic acid 8'-hydroxylase-encoding gene CYP707A2, which is required for ABA degradation, was identified as an ERF.D3 target gene through transcriptome data, RT-qPCR, dual-luciferase reporter assays and electrophoretic mobility shift assays. ERF.D3 persulfidation enhanced its transcriptional activity towards CYP707A2. Moreover, the E3 ligase RNF217 ubiquitinated ERF.D3, which may accelerate fruit ripening during the late stage of fruit development. Overall, our study provides valuable insights into the roles of a H2S-responsive ERF.D3 and its persulfidation state in delaying leaf senescence and fruit ripening and provides a link between H2S and ABA degradation.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"8 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic effects of lipid binding pockets within soluble signaling proteins: Lessons from acyl-CoA-binding and START-domain-containing proteins. 可溶性信号蛋白中脂质结合口袋的机制效应：从酰基-CoA 结合蛋白和含 START 域蛋白中汲取的教训

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-10-21 DOI: 10.1093/plphys/kiae565

Shiu-Cheung Lung,Mee-Len Chye

{"title":"Mechanistic effects of lipid binding pockets within soluble signaling proteins: Lessons from acyl-CoA-binding and START-domain-containing proteins.","authors":"Shiu-Cheung Lung,Mee-Len Chye","doi":"10.1093/plphys/kiae565","DOIUrl":"https://doi.org/10.1093/plphys/kiae565","url":null,"abstract":"While lipids serve as important energy reserves, metabolites, and cellular constituents in all forms of life, these macromolecules also function as unique carriers of information in plant communication given their diverse chemical structures. The signal transduction process involves a sophisticated interplay between messengers, receptors, signal transducers, and downstream effectors. Over the years, an array of plant signaling proteins have been identified for their crucial roles in perceiving lipid signals. However, the mechanistic effects of lipid binding on protein functions remain largely elusive. Recent literature has presented numerous fascinating models that illustrate the significance of protein-lipid interactions in mediating signaling responses. This review focuses on the category of lipophilic signaling proteins that encompass a hydrophobic binding pocket located outside of cellular membranes and provides an update on the lessons learned from two of these structures, namely the acyl-CoA-binding and START domains. It begins with a brief overview of the latest advances in understanding the functions of the two protein families in plant communication. The second part highlights five functional mechanisms of lipid ligands in concert with their target signaling proteins.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The H2S-responsive transcription factor ERF.D3 regulates tomato abscisic acid metabolism, leaf senescence, and fruit ripening. H2S反应性转录因子ERF.D3调控番茄赤霉酸代谢、叶片衰老和果实成熟。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-10-21 DOI: 10.1093/plphys/kiae560

Kangdi Hu, Meihui Geng, Lin Ma, Gaifang Yao, Min Zhang, Hua Zhang

{"title":"The H2S-responsive transcription factor ERF.D3 regulates tomato abscisic acid metabolism, leaf senescence, and fruit ripening.","authors":"Kangdi Hu, Meihui Geng, Lin Ma, Gaifang Yao, Min Zhang, Hua Zhang","doi":"10.1093/plphys/kiae560","DOIUrl":"https://doi.org/10.1093/plphys/kiae560","url":null,"abstract":"Hydrogen sulfide (H2S) is a signaling molecule that regulates plant senescence. In this study, we found that H2S delays dark-induced senescence in tomato (Solanum lycopersicum) leaves. Transcriptome and RT-qPCR analyses revealed an Ethylene Response Factor ERF.D3 is quickly induced by H2S. H2S also persulfidated ERF.D3 at amino acid residues C115 and C118. CRISPR/Cas9-mediated gene editing and gene overexpression analyses showed that ERF.D3 negatively regulates leaf senescence and fruit ripening. Abscisic acid (ABA) levels were reduced by ERF.D3 overexpression, suggesting ERF.D3 might regulate ABA metabolism. Additionally, the abscisic acid 8'-hydroxylase-encoding gene CYP707A2, which is required for ABA degradation, was identified as an ERF.D3 target gene through transcriptome data, RT-qPCR, dual-luciferase reporter assays and electrophoretic mobility shift assays. ERF.D3 persulfidation enhanced its transcriptional activity towards CYP707A2. Moreover, the E3 ligase RNF217 ubiquitinated ERF.D3, which may accelerate fruit ripening during the late stage of fruit development. Overall, our study provides valuable insights into the roles of a H2S-responsive ERF.D3 and its persulfidation state in delaying leaf senescence and fruit ripening and provides a link between H2S and ABA degradation.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GLABRA3-mediated trichome branching requires transcriptional repression of MICROTUBULE-DESTABILIZING PROTEIN25. GLABRA3 介导的毛状体分枝需要 MICROTUBULE-DESTABILIZING PROTEIN25 的转录抑制。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-10-21 DOI: 10.1093/plphys/kiae563

Wenfei Xie, Yuang Zhao, Xianwang Deng, Ruixin Chen, Zhiquan Qiang, Pedro García-Caparros, Tonglin Mao, Tao Qin

{"title":"GLABRA3-mediated trichome branching requires transcriptional repression of MICROTUBULE-DESTABILIZING PROTEIN25.","authors":"Wenfei Xie, Yuang Zhao, Xianwang Deng, Ruixin Chen, Zhiquan Qiang, Pedro García-Caparros, Tonglin Mao, Tao Qin","doi":"10.1093/plphys/kiae563","DOIUrl":"https://doi.org/10.1093/plphys/kiae563","url":null,"abstract":"Microtubules play pivotal roles in establishing trichome branching patterns, which is a model system for studying cell-shape control in Arabidopsis (Arabidopsis thaliana). However, the signaling pathway that regulates microtubule reorganization during trichome branching remains poorly understood. In this study, we report that MICROTUBULE-DESTABILIZING PROTEIN25 (MDP25) is involved in GLABRA3 (GL3)-mediated trichome branching by regulating microtubule stability. Loss of MDP25 function led to excessive trichome branching, and this phenotype in mdp25 could not be rescued by the MDP25 K7A or MDP25 K18A mutated variants. Pharmacological treatment and live-cell imaging revealed increased microtubule stability in the mdp25 mutant. Furthermore, the microtubule collar observed during trichome branching remained more intact in mdp25 compared to the WT under oryzalin treatment. Results of genetic assays further demonstrated that knocking out MDP25 rescued the reduced branching phenotype of gl3 trichomes. In gl3 trichomes, normal microtubule organization was disrupted, and microtubule stability was significantly compromised. Moreover, GL3 physically bound to the MDP25 promoter, thereby inhibiting its expression. Overexpression of GL3 negated the effects of PMDP25-driven MDP25 or its mutant proteins on trichome branching and microtubules in the mdp25 background. Overall, our study uncovers a mechanism by which GL3 inhibits MDP25 transcription, thereby influencing microtubule stability and regulating trichome branching. This mechanism provides a connection between early regulatory components and microtubules during trichome development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic effects of lipid binding pockets within soluble signaling proteins: Lessons from acyl-CoA-binding and START-domain-containing proteins. 可溶性信号蛋白中脂质结合口袋的机制效应：从酰基-CoA 结合蛋白和含 START 域蛋白中汲取的教训

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-10-21 DOI: 10.1093/plphys/kiae565

Shiu-Cheung Lung, Mee-Len Chye

{"title":"Mechanistic effects of lipid binding pockets within soluble signaling proteins: Lessons from acyl-CoA-binding and START-domain-containing proteins.","authors":"Shiu-Cheung Lung, Mee-Len Chye","doi":"10.1093/plphys/kiae565","DOIUrl":"https://doi.org/10.1093/plphys/kiae565","url":null,"abstract":"While lipids serve as important energy reserves, metabolites, and cellular constituents in all forms of life, these macromolecules also function as unique carriers of information in plant communication given their diverse chemical structures. The signal transduction process involves a sophisticated interplay between messengers, receptors, signal transducers, and downstream effectors. Over the years, an array of plant signaling proteins have been identified for their crucial roles in perceiving lipid signals. However, the mechanistic effects of lipid binding on protein functions remain largely elusive. Recent literature has presented numerous fascinating models that illustrate the significance of protein-lipid interactions in mediating signaling responses. This review focuses on the category of lipophilic signaling proteins that encompass a hydrophobic binding pocket located outside of cellular membranes and provides an update on the lessons learned from two of these structures, namely the acyl-CoA-binding and START domains. It begins with a brief overview of the latest advances in understanding the functions of the two protein families in plant communication. The second part highlights five functional mechanisms of lipid ligands in concert with their target signaling proteins.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GLABRA3-mediated trichome branching requires transcriptional repression of MICROTUBULE-DESTABILIZING PROTEIN25. GLABRA3 介导的毛状体分枝需要 MICROTUBULE-DESTABILIZING PROTEIN25 的转录抑制。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-10-21 DOI: 10.1093/plphys/kiae563

Wenfei Xie,Yuang Zhao,Xianwang Deng,Ruixin Chen,Zhiquan Qiang,Pedro García-Caparros,Tonglin Mao,Tao Qin

{"title":"GLABRA3-mediated trichome branching requires transcriptional repression of MICROTUBULE-DESTABILIZING PROTEIN25.","authors":"Wenfei Xie,Yuang Zhao,Xianwang Deng,Ruixin Chen,Zhiquan Qiang,Pedro García-Caparros,Tonglin Mao,Tao Qin","doi":"10.1093/plphys/kiae563","DOIUrl":"https://doi.org/10.1093/plphys/kiae563","url":null,"abstract":"Microtubules play pivotal roles in establishing trichome branching patterns, which is a model system for studying cell-shape control in Arabidopsis (Arabidopsis thaliana). However, the signaling pathway that regulates microtubule reorganization during trichome branching remains poorly understood. In this study, we report that MICROTUBULE-DESTABILIZING PROTEIN25 (MDP25) is involved in GLABRA3 (GL3)-mediated trichome branching by regulating microtubule stability. Loss of MDP25 function led to excessive trichome branching, and this phenotype in mdp25 could not be rescued by the MDP25 K7A or MDP25 K18A mutated variants. Pharmacological treatment and live-cell imaging revealed increased microtubule stability in the mdp25 mutant. Furthermore, the microtubule collar observed during trichome branching remained more intact in mdp25 compared to the WT under oryzalin treatment. Results of genetic assays further demonstrated that knocking out MDP25 rescued the reduced branching phenotype of gl3 trichomes. In gl3 trichomes, normal microtubule organization was disrupted, and microtubule stability was significantly compromised. Moreover, GL3 physically bound to the MDP25 promoter, thereby inhibiting its expression. Overexpression of GL3 negated the effects of PMDP25-driven MDP25 or its mutant proteins on trichome branching and microtubules in the mdp25 background. Overall, our study uncovers a mechanism by which GL3 inhibits MDP25 transcription, thereby influencing microtubule stability and regulating trichome branching. This mechanism provides a connection between early regulatory components and microtubules during trichome development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"113 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription Factor OsMYB2 Triggers Amino Acid Transporter OsANT1 expression to Regulate Rice Growth and Salt Tolerance 转录因子 OsMYB2 触发氨基酸转运体 OsANT1 的表达以调控水稻的生长和耐盐性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-10-19 DOI: 10.1093/plphys/kiae559

Shengsong Nie, Weiting Huang, Chongchong He, Bowen Wu, Honglang Duan, Jingjun Ruan, Quanzhi Zhao, Zhongming Fang

{"title":"Transcription Factor OsMYB2 Triggers Amino Acid Transporter OsANT1 expression to Regulate Rice Growth and Salt Tolerance","authors":"Shengsong Nie, Weiting Huang, Chongchong He, Bowen Wu, Honglang Duan, Jingjun Ruan, Quanzhi Zhao, Zhongming Fang","doi":"10.1093/plphys/kiae559","DOIUrl":"https://doi.org/10.1093/plphys/kiae559","url":null,"abstract":"Amino acid transporters play important roles in plant growth and stress tolerance; however, whether the abscisic acid signaling pathway regulates their transcription in rice (Oryza sativa) under salt stress remains unclear. In this study, we report that the transcription factor OsMYB2 (MYB transcription factor 2) of the abscisic acid signaling pathway mediates the expression of the gene encoding the amino acid transporter OsANT1 (aromatic and neutral amino acid transporter 1), which positively regulates growth and salt tolerance in rice. OsANT1 was mainly expressed in the leaf blade and panicle under normal conditions and transports leucine, phenylalanine, tyrosine and proline, positively regulating tillering and yield in rice. Nevertheless, salt stress induced the accumulation of abscisic acid and strongly increased the expression level of OsANT1 in the root, resulting in enhanced salt tolerance of rice seedlings, as evidenced by higher proline concentration and antioxidant-like enzyme activities and lower malondialdehyde and hydrogen peroxide concentrations. Moreover, we showed that OsMYB2 interacts with the promoter of OsANT1 and promotes its expression. Overexpression of OsMYB2 also improved tillering, yield, and salt tolerance in rice. In conclusion, our results suggest that the transcription factor OsMYB2 triggers OsANT1 expression and regulates growth and salt tolerance in rice, providing insights into the role of the abscisic acid signaling pathway in the regulatory mechanism of amino acid transporters in response to salt stress.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"33 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving rice grain shape through upstream open reading frame editing-mediated translation regulation 通过上游开放阅读框编辑介导的翻译调控改善水稻粒形

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-10-19 DOI: 10.1093/plphys/kiae557

Qingqing Yang, Wenjie Zhu, Xu Tang, Yuechao Wu, Guanqing Liu, Dongsheng Zhao, Qiaoquan Liu, Yong Zhang, Tao Zhang

引用次数: 0

A bacterial effector cleaves RIN4 to allow the dimerization and activation of its recognizing NLR. 细菌效应物会裂解 RIN4，使其识别的 NLR 二聚化并激活。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-10-18 DOI: 10.1093/plphys/kiae555

Manuel González-Fuente

引用次数: 0

Get two for the price of one: GmNF-YC4 factor mediates GmEXP7-induced root developmental changes and phosphorus starvation response in soybean. 买一送一：GmNF-YC4因子介导GmEXP7诱导的大豆根系发育变化和磷饥饿响应

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-10-18 DOI: 10.1093/plphys/kiae554

Héctor H Torres-Martínez

引用次数: 0