Plant Physiology最新文献_第9页

LACCASE35 Enhances Lignification and Resistance Against Pseudomonas syringae pv. actinidiae Infection in Kiwifruit

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-01-24 DOI: 10.1093/plphys/kiaf040

Yawei Li, Dongle Zhang, Xiaojie Wang, Fuxi Bai, Rui Li, Rongrong Zhou, Shunyuan Wu, Zemin Fang, Wei Liu, Lili Huang, Pu Liu

{"title":"LACCASE35 Enhances Lignification and Resistance Against Pseudomonas syringae pv. actinidiae Infection in Kiwifruit","authors":"Yawei Li, Dongle Zhang, Xiaojie Wang, Fuxi Bai, Rui Li, Rongrong Zhou, Shunyuan Wu, Zemin Fang, Wei Liu, Lili Huang, Pu Liu","doi":"10.1093/plphys/kiaf040","DOIUrl":"https://doi.org/10.1093/plphys/kiaf040","url":null,"abstract":"Kiwifruit bacterial canker, a highly destructive disease caused by Pseudomonas syringae pv. actinidiae (Psa), seriously affects kiwifruit (Actinidia spp.) production. Lignin deposition in infected cells serves as a defense mechanism, effectively suppressing pathogen growth. However, the underlying process remains unclear. In this study, we determined that Psa infection leads to a significant increase in S-lignin accumulation in kiwifruit. The S/G ratio in lignin was higher in a Psa-resistant cultivar than in a Psa-sensitive cultivar. Furthermore, kiwifruit laccase 35 (AcLac35), encoding an enzyme in the lignin biosynthesis pathway with characteristic laccase activity, showed tissue-specific expression in plants and was upregulated following infection by Psa. Overexpressing AcLac35 in kiwifruit leaves resulted in greater lignin content than in wild-type leaves, leading to the formation of thicker cell walls, and also activated plant-pathogen interactions and MAPK pathways, thereby enhancing resistance against Psa infection. Yeast one-hybrid assays, dual-LUC reporter assays, electrophoretic mobility shift assays, and transient injection experiments indicated that SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 9 (AcSPL9) can bind to the AcLac35 promoter, thereby positively regulating its expression. Moreover, overexpression of AcSPL9 increased lignin accumulation in kiwifruit leaves, enhancing resistance to Psa, while virus-induced gene silencing of AcSPL9 expression reduced this resistance. Our findings reveal the function of AsSPL9-AcLac35 in kiwifruit, providing insight into enhancing resistance against Psa in kiwifruit.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"113 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ASPARAGINE-RICH PROTEIN–LYST-INTERACTING PROTEIN5 complex regulates non-canonical AUTOPHAGY8 degradation in Arabidopsis

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-01-24 DOI: 10.1093/plphys/kiaf037

Yanying Wu, Kaikai Zhu, Si Chen, Enzhen Xing, Jiajia Li, Wenqi Tian, Ming Gao, Jiaxin Kong, Danni Zheng, Xue Wang, Weihong Zhou, Shuzhen Men, Xinqi Liu

{"title":"The ASPARAGINE-RICH PROTEIN–LYST-INTERACTING PROTEIN5 complex regulates non-canonical AUTOPHAGY8 degradation in Arabidopsis","authors":"Yanying Wu, Kaikai Zhu, Si Chen, Enzhen Xing, Jiajia Li, Wenqi Tian, Ming Gao, Jiaxin Kong, Danni Zheng, Xue Wang, Weihong Zhou, Shuzhen Men, Xinqi Liu","doi":"10.1093/plphys/kiaf037","DOIUrl":"https://doi.org/10.1093/plphys/kiaf037","url":null,"abstract":"The endocytic and autophagic pathways play important roles in abiotic stress responses and maintaining cellular homeostasis in plants. Asparagine Rich Proteins (NRPs) are plant-specific stress-responsive proteins that are involved in many abiotic stress-related signaling pathways. We previously demonstrated that NRP promotes PIN FORMED 2 (PIN2) vacuolar degradation to maintain PIN2 homeostasis under abscisic acid (ABA) treatment in Arabidopsis (Arabidopsis thaliana). However, the molecular function and mechanism of NRP in cellular vesicle trafficking remain unknown. In this study, we report that NRP directly interacts with LIP5 and ATG8, critical components of the endocytic and autophagic pathways, respectively. Genetic analyses show that NRP overexpression rescues canonical autophagy defects in a LIP5-dependent manner. Cellular and biochemical evidence indicates that NRP-LIP5 recruits ATG8 to multivesicular bodies for further vacuolar degradation, implying that a novel NRP-mediated endocytic pathway is utilized to compensate for the canonical autophagy defects that occur during plant stress responses. These findings provide insights into the crosstalk between the endocytic and autophagic pathways and uncover a function of ATG8 distinct from its canonical role in autophagy. The mechanism revealed here confers an evolutionary advantage to plants and provides a molecular basis for breeding crops with greater stress tolerance.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"49 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphorylation-dependent VaMYB4a regulates cold stress in grapevine by inhibiting VaPIF3 and activating VaCBF4

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-01-24 DOI: 10.1093/plphys/kiaf035

Qinhan Yu, Qiaoling Zheng, Chang Liu, Junxia Zhang, Yaping Xie, Wenkong Yao, Jiaxin Li, Ningbo Zhang, Xinyi Hao, Weirong Xu

{"title":"Phosphorylation-dependent VaMYB4a regulates cold stress in grapevine by inhibiting VaPIF3 and activating VaCBF4","authors":"Qinhan Yu, Qiaoling Zheng, Chang Liu, Junxia Zhang, Yaping Xie, Wenkong Yao, Jiaxin Li, Ningbo Zhang, Xinyi Hao, Weirong Xu","doi":"10.1093/plphys/kiaf035","DOIUrl":"https://doi.org/10.1093/plphys/kiaf035","url":null,"abstract":"Cold stress severely impacts the quality and yield of grapevine (Vitis L.). In this study, we extend our previous work to elucidate the role and regulatory mechanisms of Vitis amurensis MYB transcription factor 4a (VaMYB4a) in grapevine's response to cold stress. Our results identified VaMYB4a as a key positive regulator of cold stress. We demonstrated that VaMYB4a undergoes phosphorylation by V. amurensis CBL-interacting protein kinase 18 (VaCIPK18) under cold stress, a process that activates VaMYB4a transcriptional activity. Using ChIP-seq, we performed a comprehensive genomic search to identify downstream components that interact with VaMYB4a, leading to the discovery of a basic helix-loop-helix (bHLH) transcription factor, V. amurensis phytochrome-interacting factor 3 (VaPIF3). VaMYB4a attenuated the transcriptional activity of VaPIF3 through a phosphorylation-dependent interaction under cold conditions. Furthermore, VaPIF3, which interacts with and inhibits V. amurensis C-repeat binding factor 4 (VaCBF4, a known positive regulator of cold stress), has its activity attenuated by VaMYB4a, which mediates the modulation of this pathway. Notably, VaMYB4a also interacted with and promoted the expression of VaCBF4 in a phosphorylation-dependent manner. Our study shows that VaMYB4a positively modulates cold tolerance in plants by simultaneously downregulating VaPIF3 and upregulating VaCBF4. These findings provide a nuanced understanding of the transcriptional response in grapevine under cold stress and contribute to the broader field of plant stress physiology.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"34 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BRASSINAZOLE RESISTANT 1 delays photoperiodic flowering by repressing CONSTANS transcription 油菜素唑抗性1通过抑制CONSTANS转录延迟光周期开花

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-01-23 DOI: 10.1093/plphys/kiaf032

Xingwen Xu, Wenbo Jiang, Yangbo Chen, Hao Tian, Zijian Yang, Shuo Liu, Xiaopeng Li, Chunhui Song, Zhangli Ye, Wei Guo, Dongdong Kong, Congcong Hou, Legong Li, Liangyu Liu

引用次数: 0

Toll/interleukin-1 receptor-only genes contribute to immune responses in maize Toll/白介素-1受体基因参与玉米的免疫应答

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-01-23 DOI: 10.1093/plphys/kiaf030

Qiang Zhang, Derong Gao, Lei Tian, Kirstin Feussner, Bin Li, Long Yang, Qin Yang, Yuelin Zhang, Xin Li, Ivo Feussner, Fang Xu

{"title":"Toll/interleukin-1 receptor-only genes contribute to immune responses in maize","authors":"Qiang Zhang, Derong Gao, Lei Tian, Kirstin Feussner, Bin Li, Long Yang, Qin Yang, Yuelin Zhang, Xin Li, Ivo Feussner, Fang Xu","doi":"10.1093/plphys/kiaf030","DOIUrl":"https://doi.org/10.1093/plphys/kiaf030","url":null,"abstract":"Proteins with Toll/interleukin-1 receptor (TIR) domains are widely distributed in both prokaryotes and eukaryotes, serving as essential components of immune signaling. Although monocots lack the major TIR-nucleotide-binding (NB)-leucine-rich repeat (LRR)-type (TNL) immune receptors, they possess a small number of TIR-only proteins, the function of which remains largely unknown. In the monocot maize (Zea mays), there are three conserved TIR-only genes in the reference genome, namely ZmTIR1 to ZmTIR3. A genome-wide scan for TIR genes and comparative analysis revealed that these genes exhibit low sequence diversity and do not show copy number variation among 26 diverse inbred lines. ZmTIR1 and ZmTIR3, but not ZmTIR2, specifically trigger cell death and defense gene expression when overexpressed in Nicotiana benthamiana leaves. These responses depend on the critical glutamic acid and cysteine residues predicted to be essential for TIR-mediated NADase and 2’,3’-cAMP/cGMP synthetase activity, respectively, as well as the key TIR downstream regulator Enhanced Disease Susceptibility 1 (EDS1). Overexpression of ZmTIR3 in N. benthamiana produces signaling molecules, including 2’cADPR, 2’,3’-cAMP and 2’,3’-cGMP, a process that requires the enzymatic glutamic acid and cysteine residues of ZmTIR3. ZmTIR expression in maize is barely detectable under normal conditions, but is substantially induced by different pathogens. Importantly, the maize Zmtir3 knockout mutant exhibits enhanced susceptibility to the fungal pathogen Cochliobolus heterostrophus, highlighting the role of ZmTIR3 in maize immunity. Overall, our results unveil the function of the maize ZmTIRs. We propose that the pathogen-inducible ZmTIRs play an important role in maize immunity, likely through their enzymatic activity and via EDS1-mediated signaling.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"32 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sphingolipid remodeling in the plasma membrane is essential for osmotic stress tolerance in Arabidopsis 质膜鞘脂重塑对拟南芥的渗透胁迫耐受性至关重要

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-01-23 DOI: 10.1093/plphys/kiaf031

Yong-Kang Li, Yu-Meng Zhang, Guang-Yi Dai, Yi-Li Chen, Ding-Kang Chen, Nan Yao

{"title":"Sphingolipid remodeling in the plasma membrane is essential for osmotic stress tolerance in Arabidopsis","authors":"Yong-Kang Li, Yu-Meng Zhang, Guang-Yi Dai, Yi-Li Chen, Ding-Kang Chen, Nan Yao","doi":"10.1093/plphys/kiaf031","DOIUrl":"https://doi.org/10.1093/plphys/kiaf031","url":null,"abstract":"Osmotic stress caused by drought, salinity, or cold conditions is an important abiotic factor that decreases membrane integrity and causes cell death, thus decreasing plant growth and productivity. Remodeling cell membrane composition via lipid turnover can counter the loss of membrane integrity and cell death caused by osmotic stress. Sphingolipids are important components of eukaryotic membrane systems; however, how sphingolipids participate in plant responses to osmotic stress remains unclear. Here, we characterized the role of the glucosylceramidase (GCD) AtGCD1 (encoded by At1g33700) in sphingolipid remodeling and acclimation to osmotic stress in Arabidopsis (Arabidopsis thaliana). AtGCD1–AtGCD4 are Arabidopsis homologs of human nonlysosomal glucosylceramidase. We determined that AtGCD1 functions as a glucosylceramidase and localizes to the plasma membrane and that recombinant AtGCD1 has no substrate preference for acyl chain length. Moreover, AtGCD1 and AtGCD3 (At4g10060) are essential for osmotic stress tolerance in Arabidopsis. In cells treated with mannitol, AtGCD1 and AtGCD3 hydrolyzed glucosylceramides to ceramides, leading to decreased glucosylceramide contents and increased glycosyl inositol phosphoceramide contents. We observed a substantial change in the molecular order of lipids and membrane tension at the plasma membrane of the Arabidopsis gcd1 gcd3 double mutant, indicating that glucosylceramidases compensate for changes in membrane properties to stabilize the membrane during osmotic stress. Notably, we found that loss of GCD1 and GCD3 enhanced plant resistance to beet armyworm (Spodoptera exigua). Our results suggest that sphingolipid remodeling regulates the physicochemical properties of cellular membranes during plant stress responses.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic photosynthetic labeling and carbon-positional mass spectrometry monitor in vivo Rubisco carbon assimilation rates. 动态光合标记和碳定位质谱监测体内Rubisco碳同化率。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-01-21 DOI: 10.1093/plphys/kiaf020

Yogeswari Rajarathinam,Luisa Wittemeier,Kirstin Gutekunst,Martin Hagemann,Joachim Kopka

{"title":"Dynamic photosynthetic labeling and carbon-positional mass spectrometry monitor in vivo Rubisco carbon assimilation rates.","authors":"Yogeswari Rajarathinam,Luisa Wittemeier,Kirstin Gutekunst,Martin Hagemann,Joachim Kopka","doi":"10.1093/plphys/kiaf020","DOIUrl":"https://doi.org/10.1093/plphys/kiaf020","url":null,"abstract":"RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE (RUBISCO) is the most abundant enzyme and CO2 bio-sequestration system on Earth. Its in vivo activity is usually determined by 14CO2 incorporation into 3-phosphoglycerate (3PGA). However, the radiometric analysis of 3PGA does not distinguish carbon positions. Hence, RUBISCO activity that fixes carbon into the 1-C position of 3PGA and Calvin-Benson-Bassham (CBB) cycle activities that redistribute carbon into its 2-C and 3-C positions are not resolved. This study aims to develop technology that differentiates between these activities. In source fragmentation of gas chromatography-mass spectrometry (GC-MS) enables paired isotopologue distribution analyses of fragmented substructures and the complete metabolite structure. GC-MS measurements after dynamic photosynthetic 13CO2 labelling allowed quantification of the 13C fractional enrichment (E13C) and molar carbon assimilation rates (A13C) at carbon position 1-C of 3PGA by combining E13C from carbon positions 2,3-C2 and 1,2,3-C3 with quantification of 3PGA concentrations. We validated the procedure using two GC-time of flight (TOF)-MS instruments, operated at nominal or high mass resolution, and tested the expected 3PGA positional labelling by in vivo glycolysis of positional labelled glucose isotopomers. Mutant analysis of the highly divergent GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASEs (GAPDH1 and 2) from Synechocystis sp. PCC 6803 revealed full inactivation of the CBB cycle with maintained RUBISCO activity in Δgapdh2 and a CBB cycle modulating role of GAPDH1 under fluctuating CO2 supply. RUBISCO activity in the CBB-deficient Δgapdh2 can re-assimilate CO2 released by catabolic pathways. We suggest that RUBISCO activity in Synechocystis can scavenge carbon lost through the pentose phosphate pathway or other cellular decarboxylation reactions.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"6 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142991831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The actin cytoskeleton regulates danger-associated molecular pattern signaling and PEP1 RECEPTOR1 internalization 肌动蛋白细胞骨架调节危险相关的分子模式信号和PEP1受体1内化

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-01-17 DOI: 10.1093/plphys/kiaf023

Hongping Qian, Xinxiu Zuo, Yi Man, Changwen Xu, Pengyun Luo, Lijuan Yao, Ruohan Geng, Binghe Wang, Shihui Niu, Jinxing Lin, Yaning Cui

{"title":"The actin cytoskeleton regulates danger-associated molecular pattern signaling and PEP1 RECEPTOR1 internalization","authors":"Hongping Qian, Xinxiu Zuo, Yi Man, Changwen Xu, Pengyun Luo, Lijuan Yao, Ruohan Geng, Binghe Wang, Shihui Niu, Jinxing Lin, Yaning Cui","doi":"10.1093/plphys/kiaf023","DOIUrl":"https://doi.org/10.1093/plphys/kiaf023","url":null,"abstract":"In plants, cytoskeletal proteins assemble into dynamic polymers that play numerous roles in diverse fundamental cellular processes, including endocytosis, vesicle trafficking, and the spatial distribution of organelles and protein complexes. Plant elicitor peptides (Peps) are damage/danger-associated molecular patterns (DAMPs) that are perceived by the receptor-like kinases PEP RECEPTOR 1 (PEPR1) and PEPR2 to enhance innate immunity and inhibit root growth in Arabidopsis (Arabidopsis thaliana). To date, however, there is little evidence that the actin cytoskeleton of the host cell participates in DAMP-induced innate immunity. Here, we demonstrated that the actin cytoskeleton alters the Pep1-triggered immune response. In addition, dual-color total internal reflection fluorescence–structured illumination microscopy (TIRF-SIM) showed that PEPR1 diffusion on the plasma membrane is closely related to the actin cytoskeleton. We performed single-particle tracking to quantify individual protein particles and found that the actin cytoskeleton notably regulates PEPR1 mobility and cluster size. More importantly, we demonstrated that actin filament reconfiguration is sufficient to inhibit Pep1-induced internalization, which alters the immune response. Taken together, these findings suggest that the actin cytoskeleton functions as an integration node for Pep1 signaling and PEPR1 endocytosis.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"70 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The vacuolar phosphatases PAP26 and HIIA2.1 hydrolyze 5’-, 3’-, and 2’-nucleotides derived from RNA degradation 液泡磷酸酶PAP26和HIIA2.1水解来自RNA降解的5 ' -、3 ' -和2 ' -核苷酸

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-01-17 DOI: 10.1093/plphys/kiaf025

Nabila Firdoos, Lukas Krumwiede, Nieves Medina Escobar, Leonie Treichel, Lisa Fischer, Marco Herde, Claus-Peter Witte

{"title":"The vacuolar phosphatases PAP26 and HIIA2.1 hydrolyze 5’-, 3’-, and 2’-nucleotides derived from RNA degradation","authors":"Nabila Firdoos, Lukas Krumwiede, Nieves Medina Escobar, Leonie Treichel, Lisa Fischer, Marco Herde, Claus-Peter Witte","doi":"10.1093/plphys/kiaf025","DOIUrl":"https://doi.org/10.1093/plphys/kiaf025","url":null,"abstract":"The vacuole is an important site for RNA degradation. Autophagy delivers RNA to the vacuole, where the vacuolar T2 RNase Ribonuclease 2 (RNS2) plays a major role in RNA catabolism. The presumed products of RNS2 activity are 3’-nucleoside monophosphates (3’-NMPs). Vacuolar phosphatases that carry out 3’-NMP hydrolysis are required to metabolize 3’-NMPs, but the specific players remain unknown. Using a mutant of RNS2 and mutants of the Autophagy-Related Genes 5 and 9 (atg5 and atg9), we confirmed that 3’-NMPs are products of vacuolar RNS2-mediated RNA degradation in Arabidopsis (Arabidopsis thaliana). Moreover, we identified Purple Acid Phosphatase 26 (PAP26) and Haloacid Dehalogenase (HAD) IIA2.1 (HIIA2.1) as vacuolar 3'-NMP phosphatases. Based on phylogenetic analysis, we propose systematic nomenclature for HADIIA enzymes. PAP26 and HIIA2.1 differ in their NMP specificity and activity in vitro. However, the hiia2.1 pap26 double mutant, but generally not the respective single mutants, accumulates 3’-NMPs in addition to 5’-NMPs and, surprisingly, also 2’-NMPs. These findings suggest that PAP26 and HIIA2.1 have overlapping NMP substrate spectra in vivo. Excess 3’- and 2’-NMPs accumulate in plants exposed to a prolonged night, presumably because carbon limitation enhances autophagy-mediated vacuolar RNA degradation. We conclude that vacuolar RNA catabolism releases 3’-NMPs as well as 2’-NMPs through RNS2 and other RNases that also generate 5’-NMPs. PAP26 and HIIA2.1 are required to dephosphorylate these NMPs so that they can enter general nucleotide metabolism outside the vacuole.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"77 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor SlLBD40 regulates seed germination by inhibiting cell wall remodeling enzymes during endosperm weakening 转录因子SlLBD40在胚乳弱化过程中通过抑制细胞壁重塑酶调控种子萌发

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-01-17 DOI: 10.1093/plphys/kiaf022

Jialong Zhang, Lun Liu, Danhui Dong, Jiayi Xu, Hongxin Li, Qilin Deng, Yan Zhang, Wei Huang, Haijun Zhang, Yang-Dong Guo

{"title":"The transcription factor SlLBD40 regulates seed germination by inhibiting cell wall remodeling enzymes during endosperm weakening","authors":"Jialong Zhang, Lun Liu, Danhui Dong, Jiayi Xu, Hongxin Li, Qilin Deng, Yan Zhang, Wei Huang, Haijun Zhang, Yang-Dong Guo","doi":"10.1093/plphys/kiaf022","DOIUrl":"https://doi.org/10.1093/plphys/kiaf022","url":null,"abstract":"Uniform seed germination is crucial for consistent seedling emergence and efficient seedling production. In this study, we identified a seed-expressed protein in tomato (Solanum lycopersicum), lateral organ boundaries domain 40 (SlLBD40), that regulates germination speed. CRISPR/Cas9-generated SlLBD40 knockout mutants exhibited faster germination due to enhanced seed imbibition, independent of the seed coat. The expression of SlLBD40 was induced during the imbibition process, particularly in the micropylar endosperm, suggesting its role in endosperm weakening. Gene ontology analysis of RNA-seq data indicated that differentially expressed genes were enriched in cell wall-related processes. SlLBD40 directly targeted genes encoding cell wall remodeling enzymes implicated in endosperm weakening, including expansin 6 (SlEXP6), xyloglucan endotransglucosylase/hydrolase 23 (SlXTH23), and endo-β-mannanase 1 (SlMAN1). Our findings shed light on the role of endosperm weakening in regulating seed germination and propose potential gene targets for improving germination in species constrained by endosperm strength.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"18 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0