Plant Physiology最新文献

{"title":"Pollenless endeavour: loss of a glyoxal/galactose oxidase causes male sterility in rice","authors":"Gunjan Sharma","doi":"10.1093/plphys/kiag730","DOIUrl":"https://doi.org/10.1093/plphys/kiag730","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"9 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond auxin responses: a miR160-ARF4 module regulates the biogenesis of medicinal saponins","authors":"Avilash Singh Yadav","doi":"10.1093/plphys/kiag731","DOIUrl":"https://doi.org/10.1093/plphys/kiag731","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"11 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving rice Cas12i3 variant-based adenine and cytosine base editing by different protein-fusion strategies","authors":"Xuemei Wu,Meijie Liu,Fang Yan,Dongfang Ma,Bin Ren,Huanbin Zhou","doi":"10.1093/plphys/kiag732","DOIUrl":"https://doi.org/10.1093/plphys/kiag732","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"14 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A tale of two domains: How PIF7 controls hypocotyl growth.","authors":"Hannah Rae Thomas","doi":"10.1093/plphys/kiag729","DOIUrl":"https://doi.org/10.1093/plphys/kiag729","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Horizontal gene transfer as a driving force in plant terrestrialization and adaptive evolution","authors":"Jianchao Ma, Shanshan Dong, Yun Zhou, Chun-Peng Song, Jinling Huang","doi":"10.1093/plphys/kiag733","DOIUrl":"https://doi.org/10.1093/plphys/kiag733","url":null,"abstract":"Horizontal gene transfer (HGT) has been considered as a pivotal source of genetic innovation during early plant evolution. This review synthesizes evidence that genes acquired from microbes (e.g., bacteria and fungi) by plants facilitated critical adaptations in their terrestrialization. These include stress tolerance, phytohormone signaling, cell wall biosynthesis, and defense against pathogens and herbivores. The integration of foreign genes, often following post-transfer domestication such as intron gain, underscores a dynamic evolutionary process in land plants. Furthermore, bidirectional HGT events between plants and other organisms reveal a complex “web of life”. Ultimately, HGT is a fundamental and continuous force that has shaped plant evolution, particularly during plant colonization of land.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"80 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149086553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MsNAC72 and MsbZIP29 fine-tune salt tolerance in alfalfa via oppositely regulating MsSOS2 expression","authors":"Jing Gao, Junjie Chen, Guangjing Ma, Longxing Hu, Liang Chen, Liwen Cao","doi":"10.1093/plphys/kiag726","DOIUrl":"https://doi.org/10.1093/plphys/kiag726","url":null,"abstract":"Alfalfa (Medicago sativa L.), a high-quality perennial legume forage, is essential to global agriculture and ecosystems. Expanding alfalfa production on saline-alkaline soils is critical to alleviate shortages of high-quality forage in China, but progress is limited by a shortage of salt-tolerant varieties and incomplete understanding of the underlying molecular mechanisms. As one of the most well-characterized salt tolerance pathways in plants, salt-overly-sensitive (SOS) pathway plays a pivotal role in Na+ exclusion and cellular ion balance regulation. Here, we investigated the role and regulation of MsSOS2 in alfalfa salt stress responses. Overexpression of MsSOS2 enhanced salt tolerance in transgenic alfalfa plants, evidenced by a reduced Na+/K+ ratio. Then, two salt-induced transcription factors Basic leucine zipper 29 (MsbZIP29) and NAC domain-containing protein 72 (MsNAC72) were identified as upstream regulators of MsSOS2 through yeast one-hybrid screening. Further protein-DNA interaction assays demonstrated that MsbZIP29 directly activates MsSOS2 expression, whereas MsNAC72 directly represses it. Functional analyses revealed that MsbZIP29 promotes salt tolerance, while MsNAC72 functions as a transcriptional brake, together fine-tuning ionic homeostasis under salt stress. Additionally, MsbZIP29 interacted with MsbZIP1 to amplify the transcriptional activation of MsSOS2. Consistently, overexpression of MsbZIP1 improved salt tolerance while its knockdown increased salt stress sensitivity. Collectively, our results reveal that MsNAC72 and MsbZIP29 fine-tune salt stress tolerance in alfalfa through opposing roles in regulating MsSOS2 expression. This study provides novel insights into the transcriptional network underlying salt tolerance in alfalfa, offering potential molecular targets for improving alfalfa resilience in saline environments.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"32 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149077642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VvDLK negatively regulates cold tolerance by interacting with Vv14-3-3A in grapevine","authors":"Jiang Wu, Wenjing Shi, Sifang Luo, Lucca Magnus Armlich, Yijing Wu, Qianwei Liu, Xiao Chen, Ruslan Eliseev, Niklas Karsten Kretzler, Sai Kumar Halavath, Pingyin Guan, Peter Nick","doi":"10.1093/plphys/kiag728","DOIUrl":"https://doi.org/10.1093/plphys/kiag728","url":null,"abstract":"Sudden cold snaps due to blurred seasonality are a rising challenge to agriculture in times of climate change. Swift activation of cold signaling is crucial for resilience. Dual-localization kinesins (DLK) convey the cold signal from the plasma membrane to the nucleus, where this protein modulates cold-dependent gene expression. Using the grapevine homologue as paradigm, we investigated the functional context of VvDLK. We combined live-cell and immunofluorescence imaging with pharmacological interference, physiological analysis and protein-interaction assays upon stable expression in grapevine cells and tomato, or CRISPR-Cas mediated inactivation of the tomato homologue. Overexpression of VvDLK was associated with earlier and more pronounced cold-induced elimination of microtubules in both, tomato plants as heterologous, and grapevine cells as homologous host, followed by reduced cold tolerance, evident from cellular, physiological, and molecular readouts. Conversely, inactivation of the tomato VvDLK homologue SlDLK improved cold tolerance. VvDLK interacted specifically with Vv14-3-3A, and a 14-3-3 inhibitor attenuated cold-induced microtubule loss. Overexpression of Vv14-3-3A also increased cold susceptibility. In addition, VvDLK and Vv14-3-3A both interacted with VvCBF4, while VvDLK reduced VvCBF4 protein accumulation and altered its intranuclear distribution under cold stress. Together, these findings suggest that VvDLK is associated with cold-induced microtubule elimination and attenuates cold tolerance through a signaling module involving Vv14-3-3A and VvCBF4.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"481 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149083838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Producing tastier sweetcorn by combining waxy and sweet traits.","authors":"Rose McNelly","doi":"10.1093/plphys/kiag722","DOIUrl":"https://doi.org/10.1093/plphys/kiag722","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host Genetic Variation Is Associated with Root-Associated Microbiome Assembly and Resistance to Fusarium oxysporum in Tobacco","authors":"Huan Su, Huabing Liu, Jiemeng Tao, Zechao Qu, Qiao Wang, Peng Lu, Xinhua Tian, Shizhou Yu, Kaiyan Li, Heli Shi, Mei Zuo, Bikun Xiang, Jizhong Wu, Peijian Cao, Jingjing Jin","doi":"10.1093/plphys/kiag727","DOIUrl":"https://doi.org/10.1093/plphys/kiag727","url":null,"abstract":"Plant–microbe interactions are essential for plant health, yet the genetic basis of host control over root-associated microbiome assembly in Nicotiana tabacum remains unclear. We integrated whole-genome resequencing, amplicon sequencing, and metabolomics across 50 tobacco cultivars, together with microbial genome-wide association and mediation analyses, to investigate host–microbiome covariation. Host genotype significantly influenced the diversity and composition of root-associated microbial communities, particularly in the rhizosphere. Several host loci were associated with heritable microbial taxa linked to nutrient acquisition and stress responses. Mediation analysis suggested that associations between host genetic variation and Pseudomonadaceae abundance were partly transmitted through secondary metabolites variation, particularly terpenoids, whereas this mediating effect was less pronounced for other taxa. Functional assays further showed that several genotype-associated bacterial isolates antagonized Fusarium oxysporum. Among them, Pseudomonas aeruginosa FZ1 suppressed pathogen growth, enhanced host defense responses, and reduced disease severity in greenhouse experiments. These findings suggest that host genetic variation is associated with shifts in the tobacco root microbiome and that secondary metabolites may contribute to the assembly of specific microbial taxa. The study provides a basis for future mechanistic and breeding-oriented investigations of microbiome-associated traits.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"111 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149077641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A YopJ-family effector from Ralstonia solanacearum is associated with auxin-responsive transcriptional reprogramming and bacterial wilt suppression.","authors":"Mengshu Huang, Yuhan Li, Jiahui Wu, Hongxu Zhu, Jingxin Liu, Bingsen Wang, Xiaodan Tan, Liu Yu, Qian Li, Huilan Chen, Juan Du, Botao Song","doi":"10.1093/plphys/kiag725","DOIUrl":"https://doi.org/10.1093/plphys/kiag725","url":null,"abstract":"<p><p>Effector-triggered immunity (ETI) can reshape hormone-associated transcriptional programs during plant-pathogen interactions. However, how bacterial effectors are associated with hormone-responsive defense outputs in potato remains poorly understood. This study identifies RipBR, a previously uncharacterized YopJ-family effector from Ralstonia solanacearum, as an avirulence-associated determinant in the wild potato accession Solanum albicans ALB28-1. Loss- and gain-of-function analyses demonstrated that RipBR contributes to reduced bacterial wilt symptoms and restricts bacterial colonization in potato. Mutations in conserved residues H158 and C225 strongly impaired RipBR-associated hypersensitive response induction and disease suppression, indicating their importance for RipBR function. RipBR localized to both the nucleus and cytoplasm, whereas H158R and C225S mutations were associated with altered subcellular distribution and impaired immune-associated phenotypes. Transcriptome analysis revealed that RipBR recognition was accompanied by extensive auxin-responsive transcriptional reprogramming, including induction of AUX/IAA, ARF, GH3, and SAUR genes, together with activation of the defense-associated transcription factor SaTGA1. These responses occurred without detectable increases in endogenous indole-3-acetic acid (IAA) accumulation or major auxin biosynthetic gene expression, suggesting modulation of auxin signaling rather than enhanced auxin production. Exogenous 1-naphthaleneacetic acid (NAA) treatment alleviated bacterial wilt symptoms but did not significantly reduce bacterial colonization, indicating that auxin signaling activation alone is insufficient to establish enhanced resistance. Together, these findings identify RipBR as a YopJ-family effector associated with bacterial wilt suppression in potato and reveal an association between effector recognition and auxin-responsive transcriptional reprogramming during plant immunity.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}