Plant Physiology最新文献_第9页

The fungal effector AaAlta1 inhibits PATHOGENESIS-RELATED PROTEIN10-2-mediated callose deposition and defense responses in apple 真菌效应物 AaAlta1 可抑制苹果中由病原相关蛋白 10-2 介导的胼胝质沉积和防御反应

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-26 DOI: 10.1093/plphys/kiae599

Shun Gong, Jinqi Tang, Yi Xiao, Tianzhong Li, Qiulei Zhang

{"title":"The fungal effector AaAlta1 inhibits PATHOGENESIS-RELATED PROTEIN10-2-mediated callose deposition and defense responses in apple","authors":"Shun Gong, Jinqi Tang, Yi Xiao, Tianzhong Li, Qiulei Zhang","doi":"10.1093/plphys/kiae599","DOIUrl":"https://doi.org/10.1093/plphys/kiae599","url":null,"abstract":"Apple leaf spot, caused by Alternaria alternata f. sp mali (ALT), poses a substantial threat to the global apple (Malus × domestica Borkh.) industry. Fungal effectors promote pathogen infestation and survival by interfering with plant immune responses. In our study, we investigated the secretion of effector proteins by the virulent ALT7 strain. Using mass spectrometry, we identified the effector AaAlta1, which belongs to the Alt a 1 protein family (AA1s). Further analysis confirmed that ALT7 secretes AaAlta1. AaAlta1 knockdown mutants displayed reduced pathogenicity in apple tissue culture seedlings, while overexpression strains exhibited enhanced pathogenicity compared to the wild-type ALT7 strain. Using immunoprecipitation followed by mass spectrometry, we isolated pathogenesis-related protein 10-2 (PR10-2) as an interaction partner of AaAlta1 in apple. Knockdown mutants of AaAlta1 showed increased PR10-2-mediated callose deposition in apple, a critical plant defense response. The enhanced defense responses in apple substantially reduced their susceptibility to infection by these ALT7 mutants. Our findings delineate an infection strategy whereby ALT7 secretes AaAlta1 to suppress PR10-2, thereby circumventing the apple defense system.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"21 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142718416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The RALF1-FERONIA complex phosphorylates FREE1 to attenuate abscisic acid signaling during seedling establishment RALF1-FERONIA 复合物磷酸化 FREE1，从而在幼苗形成过程中减弱脱落酸信号传导

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-23 DOI: 10.1093/plphys/kiae625

Qiong Fu, Hongbo Li, Bingqian Wang, Weijun Shen, Doushen Wu, Caiji Gao, Feng Yu

{"title":"The RALF1-FERONIA complex phosphorylates FREE1 to attenuate abscisic acid signaling during seedling establishment","authors":"Qiong Fu, Hongbo Li, Bingqian Wang, Weijun Shen, Doushen Wu, Caiji Gao, Feng Yu","doi":"10.1093/plphys/kiae625","DOIUrl":"https://doi.org/10.1093/plphys/kiae625","url":null,"abstract":"The receptor-like kinase FERONIA (FER), together with its ligand rapid alkalinization factor 1 (RALF1) peptide, plays a crucial role in regulating stress responses, including its involvement in modulating abscisic acid (ABA) signaling. FER has been shown to activate ABA Insensitive 2 (ABI2) in the cytoplasm, leading to the suppression of ABA signaling. However, its regulation of nucleus events in the ABA response remains unclear. FREE1, identified as a plant-specific component of the endosomal sorting complex required for transport (ESCRT) in eukaryotes, serves as an important negative regulator in ABA signaling. In this study, we elucidate that upon RALF1 treatment, FER phosphorylates FREE1, promoting the accumulation of FREE1 protein in the nucleus in Arabidopsis (Arabidopsis thaliana). Consequently, FREE1 suppresses ABA sensitivity by inhibiting the expression of ABA-response genes. Mutating the six identified phosphorylation sites on FREE1, mediated by FER, to non-phosphorylable residues results in reduced nucleus localization of FREE1 and increased hypersensitivity to ABA. Our data also show that these six phosphorylation sites are likely involved in regulating plant survival under salt stress. Collectively, our study not only unveils an additional function of FER in attenuating ABA signaling in the nucleus but also provides a possible insight into the role of the RALF1-FER-FREE1 module in coordinating plant growth and salt stress tolerance.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"58 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The receptor MIK2 interacts with the kinase RKS1 to control quantitative disease resistance to Xanthomonas campestris 受体MIK2与激酶RKS1相互作用，控制对野菜黄单胞菌的定量抗病性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-23 DOI: 10.1093/plphys/kiae626

Florent Delplace, Carine Huard-Chauveau, Fabrice Roux, Dominique Roby

{"title":"The receptor MIK2 interacts with the kinase RKS1 to control quantitative disease resistance to Xanthomonas campestris","authors":"Florent Delplace, Carine Huard-Chauveau, Fabrice Roux, Dominique Roby","doi":"10.1093/plphys/kiae626","DOIUrl":"https://doi.org/10.1093/plphys/kiae626","url":null,"abstract":"Molecular mechanisms underlying qualitative resistance have been intensively studied. In contrast, although quantitative disease resistance (QDR) is a common, durable and broad-spectrum form of immune responses in plants, only a few related functional analyses have been reported. The atypical kinase Resistance related KinaSe1 (RKS1) is a major regulator of QDR to the bacterial pathogen Xanthomonas campestris (Xcc) and is positioned in a robust protein-protein decentralized network in Arabidopsis (Arabidopsis thaliana). Among the putative interactors of RKS1 found by yeast two-hybrid screening, we identified the receptor-like kinase MDIS1-Interacting Receptor-like Kinase 2 (MIK2). Here, using multiple complementary strategies including protein-protein interaction tests, mutant analysis, and network reconstruction, we report that MIK2 is a component of RKS1-mediated QDR to Xcc. First, by co-localization experiments, co-immunoprecipitation (Co-IP), and bimolecular fluorescence complementation (BiFC), we validated the physical interaction between RKS1 and MIK2 at the plasma membrane. Using mik2 mutants, we showed that MIK2 is required for QDR and contributes to resistance to the same level as RKS1. Interestingly, a catalytic mutant of MIK2 interacted with RKS1 but was unable to fully complement the mik2-1 mutant phenotype in response to Xcc. Finally, we investigated the potential role of the MIK2–RKS1 complex as a scaffolding component for the coordination of perception events by constructing a RKS1–MIK2 centered protein-protein interaction network. Eight mutants corresponding to seven RKs in this network showed a strong alteration in QDR to Xcc. Our findings provide insights into the molecular mechanisms underlying the perception events involved in QDR to Xcc.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"15 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GmERFVII transcription factors upregulate PATHOGENESIS-RELATED10 and contribute to soybean cyst nematode resistance. GmERFVII 转录因子上调 PATHOGENESIS-RELATED10 并促进大豆胞囊线虫的抗性。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-22 DOI: 10.1093/plphys/kiae548

Miaomiao Deng, Lei Zhang, Chao Yang, Qian Zeng, Linlin Zhong, Xiaoli Guo

{"title":"GmERFVII transcription factors upregulate PATHOGENESIS-RELATED10 and contribute to soybean cyst nematode resistance.","authors":"Miaomiao Deng, Lei Zhang, Chao Yang, Qian Zeng, Linlin Zhong, Xiaoli Guo","doi":"10.1093/plphys/kiae548","DOIUrl":"https://doi.org/10.1093/plphys/kiae548","url":null,"abstract":"Low oxygen availability within plant cells arises during plant development but is exacerbated under environmental stress conditions. The group VII ETHYLENE RESPONSE FACTOR (ERFVII) transcription factors have been identified as pivotal regulators in the hypoxia response to abiotic stress. However, their roles in transcriptional regulation during biotic stresses remain less defined. In this study, we investigated the biological function and regulatory mechanism of soybean (Glycine max) ERFVII transcription factors during soybean cyst nematode (Heterodera glycines Ichinohe) infection. We provide evidence that soybean cyst nematode infection induces responses at the infection sites similar to those induced by hypoxia, characterized by the stabilization of ERFVII proteins and increased expression of hypoxia-responsive genes. Hypoxia pretreatment of soybeans enhances their resistance to nematode infection. We demonstrate that ERFVII members GmRAP2.12 and GmRAP2.3 act as transcriptional activators to drive the expression of GmPR10-09g, a member of the PR10 gene family highly induced by soybean cyst nematode and positively impacting nematode resistance. Transgenic hairy root analysis of nematode infection for either GmRAP2.12 or N-end rule pathway components (GmATE or GmPRT6) indicates a positive role of ERFVIIs in soybean defense responses against cyst nematode. The results of our study emphasize the important functions of GmERFVIIs in strengthening soybean's immune responses against cyst nematode by transcriptional activation of GmPR10.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protoplast transient transformation facilitates subcellular localization and functional analysis of walnut proteins. 原生质体瞬时转化有助于核桃蛋白质的亚细胞定位和功能分析。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-22 DOI: 10.1093/plphys/kiae627

Yanli Gao, Tianyu Tang, Wenhan Cao, Muhammad Ali, Qirong Zhou, Dongmei Zhu, Xiaohui Ma, Yi Cai, Qixiang Zhang, Zhengjia Wang, Dong Pei, Jianqin Huang, Jinbo Shen

{"title":"Protoplast transient transformation facilitates subcellular localization and functional analysis of walnut proteins.","authors":"Yanli Gao, Tianyu Tang, Wenhan Cao, Muhammad Ali, Qirong Zhou, Dongmei Zhu, Xiaohui Ma, Yi Cai, Qixiang Zhang, Zhengjia Wang, Dong Pei, Jianqin Huang, Jinbo Shen","doi":"10.1093/plphys/kiae627","DOIUrl":"https://doi.org/10.1093/plphys/kiae627","url":null,"abstract":"Walnut (Juglans regia), an important contributor to oil production among woody plants, encounters research constraints due to difficulties in the subcellular localization and functional analysis of its proteins. These limitations arise from the protracted fruiting cycle and the absence of a reliable transient gene transformation system and organelle markers. In this study, we established a transient expression system using walnut protoplasts and generated fluorescent-tagged organelle markers, whose localization was validated against Arabidopsis (Arabidopsis thaliana) organelle markers. The versatility of this system was demonstrated through pharmaceutical treatments, confirming its ability to determine the subcellular localization of endogenous proteins. We determined the subcellular localization of walnut oleosin proteins and explored protein-protein interactions through bimolecular fluorescence complementation (BiFC) analysis. We also explored the effects of abscisic acid (ABA) signaling on oil body morphology and the regulation of walnut WRINKLED1 (JrWRI1) in lipid biosynthesis. Overall, this stable and versatile protoplast-based transient expression system, integrated with walnut organelle markers, enhances the subcellular localization and functional studies of uncharacterized walnut proteins. This advancement accelerates research into walnut gene function and streamlines molecular breeding processes with high throughput efficiency.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Follow the calcium road: Conserved mechanisms of growth and development in Marchantia polymorpha. 沿着钙化之路Marchantia polymorpha 生长发育的保守机制。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-22 DOI: 10.1093/plphys/kiae622

Jiawen Chen, Erin Cullen

引用次数: 0

Go with the flux: Modelling accurately predicts phenotypes of Arabidopsis lipid mutants. 随波逐流：建模准确预测拟南芥脂质突变体的表型

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-22 DOI: 10.1093/plphys/kiae620

Erin Cullen, Maneesh Lingwan

引用次数: 0

Tools to understand hypoxia responses in plant tissues. 了解植物组织缺氧反应的工具。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-22 DOI: 10.1093/plphys/kiae624

Gabriele Panicucci, Pedro Barreto, Max Herzog, Sophie Lichtenauer, Markus Schwarzländer, Ole Pedersen, Daan A Weits

{"title":"Tools to understand hypoxia responses in plant tissues.","authors":"Gabriele Panicucci, Pedro Barreto, Max Herzog, Sophie Lichtenauer, Markus Schwarzländer, Ole Pedersen, Daan A Weits","doi":"10.1093/plphys/kiae624","DOIUrl":"https://doi.org/10.1093/plphys/kiae624","url":null,"abstract":"Our understanding of how low oxygen (O2) conditions arise in plant tissues and how they shape specific responses has seen major advancement in recent years. Important drivers have been (i) the discovery of the molecular machinery that underpins plant O2 sensing and (ii) a growing set of dedicated tools to define experimental conditions and assess plant responses with increasing accuracy and resolution. While some of those tools, such as the Clark-type O2 electrode, were established decades ago, recent customization has set entirely new standards and enabled novel research avenues in plant hypoxia research. Other tools, such as optical hypoxia reporters and O2 biosensor systems, have been introduced more recently. Yet, their adoption into plant hypoxia research has started to generate novel insight into hypoxia physiology at the tissue and cellular levels. The aim of this update is to provide an overview of the currently available and emerging tools for O2 hypoxia measurements in plants, with an emphasis on high-resolution analyses in living plant tissues and cells. Furthermore, it offers directions for future development and deployment of tools to aid progress with the most pressing questions in plant hypoxia research.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rooting for order: how CIKs keep lateral growth in check. 扎根秩序：CIK 如何控制横向发展。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-21 DOI: 10.1093/plphys/kiae621

Alicja B Kunkowska, Nicola Trozzi

引用次数: 0

Histone modification H3K27me3 is essential during chilling-induced flowering in Litchi chinensis 组蛋白修饰 H3K27me3 在寒冷诱导荔枝开花过程中至关重要

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-20 DOI: 10.1093/plphys/kiae619

Xifen Pan, Xingyu Lu, Lijie Huang, Zhiqun Hu, Maogen Zhuo, Yanchun Ji, Bingqi Lin, Jianqin Luo, Peitao Lü, Biyan Zhou

{"title":"Histone modification H3K27me3 is essential during chilling-induced flowering in Litchi chinensis","authors":"Xifen Pan, Xingyu Lu, Lijie Huang, Zhiqun Hu, Maogen Zhuo, Yanchun Ji, Bingqi Lin, Jianqin Luo, Peitao Lü, Biyan Zhou","doi":"10.1093/plphys/kiae619","DOIUrl":"https://doi.org/10.1093/plphys/kiae619","url":null,"abstract":"Litchi (Litchi chinensis), a prominent fruit tree in the Sapindaceae, initiates flowering in response to low autumn and winter temperatures. This study investigates the epigenetic regulation of this process, focusing on the marks histone H3 lysine 27 trimethylation (H3K27me3) and its deposition genes during the chilling-induced floral induction and initiation stages. Our genomic analysis delineated the H3K27me3 deposition landscape across the pre-floral induction (PFId), floral induction (FId), and floral initiation (FIn) stages. We identified 5,635 differentially H3K27me3-modified genes (DHGs) in buds and 4,801 DHGs in leaves. Integration of the RNA-seq and ChIP-seq datasets identified 1,001 differentially regulated genes (DRGs) in buds and 675 DRGs in leaves, offering insights into the genes potentially targeted by H3K27me3. To probe the functional role of H3K27me3, we employed GSK343, a histone H3 lysine methyltransferase inhibitor. Treatment with GSK343 during the chilling-induced flowering process led to reduced H3K27me3 deposition at the TREHALOSE-6-PHOSPHATE SYNTHASE 1 (LcTPS1) and FRIGIDA (LcFRI) loci, resulting in increased gene expression. This manipulation delayed flowering and reduced flowering rates, confirming the pivotal role of H3K27me3 in chilling-induced flowering in litchi. Gene co-expression network analysis identified SHORT VEGETATIVE PHASE 10 (LcSVP10) as a crucial regulator in litchi flowering. Overexpression of LcSVP10 in Arabidopsis thaliana delayed flowering, indicating a conserved function in flowering time control. Our results elucidate the molecular and epigenetic mechanisms that govern floral induction in litchi and highlight the potential of epigenetic modifications to regulate flowering time in horticultural plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"99 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0