Plant Physiology最新文献_第9页

Exclusive and hypersensitive stomatal control by blue-light in Equisetum 蓝光对木贼草气孔的排他性和超敏性控制

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-06 DOI: 10.1093/plphys/kiaf238

Scott A M McAdam, Anju Manandhar, Ian M Rimer, Daniela Aros-Mualin

{"title":"Exclusive and hypersensitive stomatal control by blue-light in Equisetum","authors":"Scott A M McAdam, Anju Manandhar, Ian M Rimer, Daniela Aros-Mualin","doi":"10.1093/plphys/kiaf238","DOIUrl":"https://doi.org/10.1093/plphys/kiaf238","url":null,"abstract":"Most ferns are adapted to lower-light environments and have relatively low rates of leaf gas exchange compared to seed plants. Recent studies suggest that certain fern groups adapted to full-sun, yet ever-wet, environments have evolved novel stomatal regulatory mechanisms, particularly in response to light, enabling higher rates of leaf gas exchange. Among these lineages, the genus Equisetum, a morphologically and ecologically distinctive group of ferns, remains poorly understood in terms of stomatal physiology. Here, we investigated stomatal control by light in Equisetum by combining observations of stomatal conductance in the field with measurements of canopy conductance in a controlled environment and stem-level gas exchange responses to varying wavelengths and intensities of light. We found that Equisetum stomatal closure in the dark occurs over five minutes, which is extremely fast compared to other ferns. Additionally, Equisetum has lost a stomatal response to red light, with stomata exclusively regulated by blue light. This novel regulation of stomata by blue light and rapid stomatal closure in the dark results in strong co-ordination between canopy conductance and light intensity at the end of the day. Our results have implications for understanding the regulation of stomata by light and suggest that there is considerable ecologically relevant diversity in stomatal regulation within ferns.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"18 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144228454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two MYB transcription factors interact to inhibit the expression of cell wall metabolism and starch degradation genes in banana 两种MYB转录因子相互作用抑制香蕉细胞壁代谢和淀粉降解基因的表达

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-06 DOI: 10.1093/plphys/kiaf239

Ting-ting Luo, Hui Zhang, Hai-ke Tan, Li-ting Zhang, Wei Wei, Wei Shan, Jian-fei Kuang, Jian-ye Chen, Wang-jin Lu, Ying-ying Yang

{"title":"Two MYB transcription factors interact to inhibit the expression of cell wall metabolism and starch degradation genes in banana","authors":"Ting-ting Luo, Hui Zhang, Hai-ke Tan, Li-ting Zhang, Wei Wei, Wei Shan, Jian-fei Kuang, Jian-ye Chen, Wang-jin Lu, Ying-ying Yang","doi":"10.1093/plphys/kiaf239","DOIUrl":"https://doi.org/10.1093/plphys/kiaf239","url":null,"abstract":"Banana (Musa acuminata, AAA group) fruit softening severely affects postharvest quality, yet the molecular regulatory networks governing this process remain incompletely understood. In this study, we found that the overexpression of MaMYB44 in bananas and tomatoes delayed fruit firmness loss and starch degradation. In addition, MaMYB44 interacted with MaMYB73 (another member of the R2R3-MYB transcription factor family), which also functioned as a repressor of fruit firmness loss and starch degradation. To elucidate the regulatory network of these transcription factors, we performed a genome-wide co-target gene analysis using DNA affinity purification sequencing (DAP-Seq). The results showed that MaMYB44 and MaMYB73 co-targeted the cell wall metabolism gene expansin A15 (MaEXPA15) and the starch degradation gene β-amylase3 (MaBAM3). Notably, the interaction of MaMYB44 and MaMYB73 enhanced their binding affinity and inhibitory effects on MaEXPA15 and MaBAM3. This study uncovers a regulatory mechanism in which MaMYB44 and MaMYB73 form a complex to inhibit the expression of genes involved in fruit firmness loss and starch degradation. These findings provide perspectives on controlling fruit softening and idenitify important targets for improving postharvest quality.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"15 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Live-cell imaging of a plant virus replicase during infection using a genetically encoded, antibody-based probe 植物病毒复制酶在感染期间的活细胞成像使用基因编码，基于抗体的探针

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-06 DOI: 10.1093/plphys/kiaf240

Chiyusa Ishihara, Nobumitsu Sasaki, Yasuhiko Matsushita, Tomohiro Tsunoda, Tsutomu Arie, Richard S Nelson, Ken Komatsu

{"title":"Live-cell imaging of a plant virus replicase during infection using a genetically encoded, antibody-based probe","authors":"Chiyusa Ishihara, Nobumitsu Sasaki, Yasuhiko Matsushita, Tomohiro Tsunoda, Tsutomu Arie, Richard S Nelson, Ken Komatsu","doi":"10.1093/plphys/kiaf240","DOIUrl":"https://doi.org/10.1093/plphys/kiaf240","url":null,"abstract":"Replication of plant positive-strand RNA viruses occurs in association with intracellular membranes. To date, no versatile technology has been developed to directly label and visualize an active replicase in live plant cells because, in general, replicase function is not retained when it is fused to a protein for fluorescence imaging. We developed a technique to label and image a plant virus replicase during infection using the transiently expressed human influenza hemagglutinin (HA) frankenbody (FB), an antibody fragment that binds the HA epitope. The function of FB was demonstrated by visualizing the targeting of mCherry-fused FB (FB-mCherry) to an HA-tagged and GFP-fused endoplasmic reticulum (ER) marker protein in its native location. The combination of a two-component inducible system with the FB probe enabled FB-mCherry to label an HA epitope-tagged, functional replicase of Plantago asiatica mosaic virus (PlAMV) during its infection in Nicotiana benthamiana cells without affecting virus replication efficiency. The HA-tagged PlAMV replicase forms punctate structures associated with the ER and plasmodesmata and is localized in the vicinity of dsRNA, a hallmark of a viral replication complex. This application of epitope tag-binding intracellular probes for live subcellular imaging in plant cells offers insights into the localization dynamics of an active plant virus replicase during infection and the potential to co-localize host factors with the active replicase in situ.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"34 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STAY-GREEN overexpression in dark-incubated leaves promotes the formation of transitional chromoplast-like plastids STAY-GREEN的过度表达在深色培养的叶片中促进过渡性色质体样质体的形成

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-06 DOI: 10.1093/plphys/kiaf242

Hui Zheng, Salvador Torres-Montilla, Xingqi Huang, Manuel Rodríguez-Concepción, Shan Lu

{"title":"STAY-GREEN overexpression in dark-incubated leaves promotes the formation of transitional chromoplast-like plastids","authors":"Hui Zheng, Salvador Torres-Montilla, Xingqi Huang, Manuel Rodríguez-Concepción, Shan Lu","doi":"10.1093/plphys/kiaf242","DOIUrl":"https://doi.org/10.1093/plphys/kiaf242","url":null,"abstract":"The transition of chloroplasts into chromoplasts and gerontoplasts during fruit ripening and leaf senescence, respectively, involves chlorophyll breakdown and chloroplast deterioration. Chlorophyll removal is carried out by several enzymes. Among them, the Mg-dechelatase STAY-GREEN (SGR) catalyzes the first step of chlorophyll degradation. The tomato green-flesh (gf) and pepper chlorophyll retainer (cl) mutants are SGR loss-of-function mutants that maintain high levels of thylakoid structures during chromoplast development in ripening fruits. Here, by overexpressing SGR in non-illuminated Nicotiana benthamiana leaves, we demonstrated that SGR triggers the onset of chloroplast deterioration, resulting in the formation of orange leaf sectors containing plastids with carotenoid-bearing structures, although carotenoid production is not induced. Metabolite, microscopy, and transcriptome analyses suggested the onset of chloroplast senescence, indicating a possible transitional plastid stage in SGR-overexpressing regions. Overall, our work demonstrates the remarkable ability of plant plastids to adapt their ultrastructure to accommodate the precise metabolic composition of various developmental and environmental contexts.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"34 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144237176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The m6A reader SlYTH1 regulates flavor-related volatiles biosynthesis via affecting mRNA stability and translation in tomato fruit m6A解读子SlYTH1通过影响番茄果实中mRNA的稳定性和翻译来调控风味相关挥发物的生物合成

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-06 DOI: 10.1093/plphys/kiaf245

Ying Gao, Hanxiao Bian, Hanqing Wang, Jingyu Wang, Bingbing Ye, Chi Zhang, Min Xu, Yu Pan, Zhiping Deng, Zhengguo Li, Kunsong Chen, Bo Zhang

{"title":"The m6A reader SlYTH1 regulates flavor-related volatiles biosynthesis via affecting mRNA stability and translation in tomato fruit","authors":"Ying Gao, Hanxiao Bian, Hanqing Wang, Jingyu Wang, Bingbing Ye, Chi Zhang, Min Xu, Yu Pan, Zhiping Deng, Zhengguo Li, Kunsong Chen, Bo Zhang","doi":"10.1093/plphys/kiaf245","DOIUrl":"https://doi.org/10.1093/plphys/kiaf245","url":null,"abstract":"N 6-methyladenosine (m6A) is the most prevalent RNA epigenetic modification in eukaryotes, catalyzed by methyltransferases (writers), removed by demethylases (erasers), and recognized by binding proteins (readers). While previous studies have established the essential role of m6A homeostasis in regulating fruit ripening, the post-transcriptional mechanisms underlying m6A-mediated quality trait formation remain poorly understood. Following our recent discovery of a YT521B homology (YTH) domain-containing m6A reader gene, SlYTH2, as a translational repressor for the production of tomato (Solanum lycopersicum) aroma volatiles, we reveal here a distinct regulatory effect through knockout of SlYTH1, which specifically reduces flavor-related volatiles in tomato fruit without affecting the days from anthesis to fruit color break, ethylene production, or firmness. Notably, this finding contrasts with the role of SlYTH2, which negatively regulates fruit aroma, highlighting the opposing effects of SlYTH1 and SlYTH2 in modulating fruit aroma. Mechanistically, SlYTH1 binds to m6A-modified mRNA targets both in vitro and in vivo, performing dual roles in maintaining mRNA stability and promoting translation. Specifically, the loss of SlYTH1 function accelerated the decline in the transcript and protein levels of two key targets, SlBCAT1 and SlTNH1, which are essential for volatile biosynthesis. This study provides insights into the role of m6A modification in regulating fruit flavor quality during ripening. Furthermore, it identifies SlYTH1 as a potential genetic target for improving fruit flavor without altering the timing of ripening.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"478 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential impact of dawn and dusk watering on tomato metabolism and biomass allocation 黎明和黄昏浇水对番茄代谢和生物量分配的差异影响

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-06 DOI: 10.1093/plphys/kiaf227

João Antonio Siqueira, Auxiliadora O Martins, Thiago Wakin, Lucas Realto, Marcelle Ferreira-Silva, Julia Wakin, Daniel Gomes Coelho, Agustin Zsögön, Alisdair R Fernie, Adriano Nunes-Nesi, Wagner L Araújo

{"title":"Differential impact of dawn and dusk watering on tomato metabolism and biomass allocation","authors":"João Antonio Siqueira, Auxiliadora O Martins, Thiago Wakin, Lucas Realto, Marcelle Ferreira-Silva, Julia Wakin, Daniel Gomes Coelho, Agustin Zsögön, Alisdair R Fernie, Adriano Nunes-Nesi, Wagner L Araújo","doi":"10.1093/plphys/kiaf227","DOIUrl":"https://doi.org/10.1093/plphys/kiaf227","url":null,"abstract":"Water supply constraints limit crop yield across seasons and locations, restricting food production under diverse climate scenarios. Irrigation schedules that align with plant water demand at specific times of the day have been proposed to overcome limitations in the water supply. Watering crops in the morning has been associated with reductions in productivity, although the mechanisms underlying this phenomenon remain poorly understood. Here, we demonstrate that watering tomato (Solanum lycopersicum) at different times of the day alters the transcriptional patterns of genes controlling flowering induction. Dawn-watering (DAW) triggered a strong repression of the Single Flower Truss (SFT) gene, leading to metabolite accumulation and delayed development. Dusk-watered (DUW) plants showed increased fruit production compared to DAW-treated plants. Our findings highlight how metabolism and development in tomato are remodeled by the timing of watering, suggesting strategies to enhance tomato water-use efficiency (WUE) by incorporating time-specific watering practices in agriculture.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"12 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144228628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CitSAR-mediated coordination of sucrose and citrate metabolism in citrus fruits citsar介导的柑橘类水果蔗糖和柠檬酸盐代谢的协调

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-06 DOI: 10.1093/plphys/kiaf228

Shengchao Liu, Yinchun Li, Yijing Fan, Mengjie Xu, Ziyi Huang, Dengliang Wang, Chongde Sun, Shaojia Li

{"title":"CitSAR-mediated coordination of sucrose and citrate metabolism in citrus fruits","authors":"Shengchao Liu, Yinchun Li, Yijing Fan, Mengjie Xu, Ziyi Huang, Dengliang Wang, Chongde Sun, Shaojia Li","doi":"10.1093/plphys/kiaf228","DOIUrl":"https://doi.org/10.1093/plphys/kiaf228","url":null,"abstract":"Soluble sugars and organic acids are the primary determinants of flavor quality and consumer appeal in fruits. In citrus, sucrose and citric acid dominate the sugar and acid profiles, directly influencing sensory attributes and market preference. Here, we identified the sugar and acid regulator (CitSAR) through transcriptome analysis by investigating sugar and acid levels at the fruit development stage of two mandarin orange (Citrus reticulata Blanco) cultivars: ‘Ponkan’ (PK), and a bud sport mutant of PK (mPK) that exhibits a higher sugar and lower acid profile. Functional characterization revealed that CitSAR orchestrates a hierarchical transcriptional network governing sucrose and citrate metabolism, as demonstrated by overexpression assays in citrus callus and tomato. Mechanistic studies using dual-luciferase reporter assays and electrophoretic mobility shift assays (EMSA) confirmed that CitSAR binds directly to the GCC-box in the promoters of CitSPS4 and CitGAD4, activating their expression. Our findings corroborate the important role of CitSAR in modulating sucrose and citrate homeostasis and provide a molecular framework for enhancing citrus sensory quality.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"5 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144228622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide cross-links calcium signals to enhance cold tolerance via inhibiting calmodulin expression in watermelon 一氧化氮交联钙信号，通过抑制钙调素表达增强西瓜的耐寒性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-06 DOI: 10.1093/plphys/kiaf243

Yanliang Guo, Jiayue Li, Lingling Liu, Wanbang Yang, Yijia Zhou, Chunhua Wei, Jianxiang Ma, Yong Zhang, Jianqiang Yang, Yunqi Liu, Huijun Zhang, Li Yuan, Xian Zhang, Hao Li

{"title":"Nitric oxide cross-links calcium signals to enhance cold tolerance via inhibiting calmodulin expression in watermelon","authors":"Yanliang Guo, Jiayue Li, Lingling Liu, Wanbang Yang, Yijia Zhou, Chunhua Wei, Jianxiang Ma, Yong Zhang, Jianqiang Yang, Yunqi Liu, Huijun Zhang, Li Yuan, Xian Zhang, Hao Li","doi":"10.1093/plphys/kiaf243","DOIUrl":"https://doi.org/10.1093/plphys/kiaf243","url":null,"abstract":"Nitric oxide (NO) is a pivotal gaseous signaling molecule that plays a critical role in regulating plant tolerance to cold stress; however, the underlying mechanisms of signal transduction remain poorly elucidated. In this study, knockout of nitrate reductase 1 (ClNR1), a crucial gene for NO biosynthesis, led to reduced cold tolerance in watermelon (Citrullus lanatus), accompanied by downregulation of cycle nucleotide-gated channel (ClCNGC) 20, a key Ca2+-permeable channel gene, decreased Ca2+ influx, and upregulation of calmodulin (ClCaM) 2/5/7. Conversely, application of the NO donor SNP exhibited contrasting effects compared to NR1 knockout. Silencing ClCNGC20 counteracted SNP-induced Ca2+ influx, downregulation of ClCaM 2/5/7, and cold tolerance. Silencing ClCaM2/5/7 alleviated the inhibition on C-REPEAT BINDING FACTOR (ClCBF) expression and cold tolerance induced by ClNR1 knockout or ClCNGC20 silencing. Multiple experimental approaches revealed the interactions between ClCaM2/5/7 and voltage-dependent anion channel (ClVDAC) 1 proteins. Overexpression of ClVDAC1 hindered the induction of ClCBF expression and cold tolerance triggered by SNP or CaCl2, whereas ClVDAC1 silencing mitigated the inhibition on ClCBF expression and cold tolerance caused by ClNR1 knockout or ClCNGC20 silencing. Additionally, cold stress rapidly triggered Ca2+ influx, which stimulated NO production. These findings suggest that Ca2+ influx promotes NO generation, which leads to further Ca2+ influx via upregulating ClCNGC20, forming a positive feedback loop that enhances cold tolerance. Furthermore, ClCaM2/5/7 interacts with ClVDAC1 to negatively regulate the NO- and Ca2+ signaling-mediated CBF pathway and subsequent cold tolerance.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"18 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The wheat lesion mimic 34 mutant shows lesion mimicry and enhanced reactive oxygen species-mediated immunity to powdery mildew 小麦病变模拟34突变体表现出病变模拟和增强的活性氧介导的白粉病免疫

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-05 DOI: 10.1093/plphys/kiaf220

Yu Fang, Sitong Liu, Run Ding, Feilong Guo, Hao Jiang, Jun Wei, Yue Yang, Yi Han, Yu Li, Lifeng Gao, Hong Cao, Yongxiu Liu

{"title":"The wheat lesion mimic 34 mutant shows lesion mimicry and enhanced reactive oxygen species-mediated immunity to powdery mildew","authors":"Yu Fang, Sitong Liu, Run Ding, Feilong Guo, Hao Jiang, Jun Wei, Yue Yang, Yi Han, Yu Li, Lifeng Gao, Hong Cao, Yongxiu Liu","doi":"10.1093/plphys/kiaf220","DOIUrl":"https://doi.org/10.1093/plphys/kiaf220","url":null,"abstract":"Lesion mimicry typically manifests as leaf disease-like symptoms in the absence of pathogen infection, characterized by a hypersensitive response (HR), which is intricately linked to plant disease resistance. In this study, the wheat (Triticum aestivum L) mutant lesion mimic 34 (lm34), harboring a recessive mutation in the Zhongyou 206 (ZY206) background, was comprehensively investigated. The lm34 mutation was mapped to a 140-kb region on chromosome 4AL through bulked segregant exome capture sequencing (BSE-Seq) and fine-mapping. Sequence comparison and phenotypic analysis of the lm34Jing mutant revealed that TraesCS4A03G1225400, encoding a typical CC-NB-LRR protein, is the causal gene in lm34. Transient expression assays in Nicotiana benthamiana leaves indicated that the TaLM34 extended coiled coil (eCC) domain can induce cell death. Furthermore, lm34 mutants display elevated reactive oxygen species (ROS) levels and significantly increased expression of pathogenesis-related (PR) genes (PR2 and PR10), leading to enhanced powdery mildew resistance. Our findings suggest that TaLM34 can serve as a potential target for the development of wheat cultivars with improved disease resistance. Overall, our study sheds light on the molecular mechanisms underlying wheat disease resistance and cell death.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"402 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144228631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutations in the floral regulator gene HUA2 restore flowering to the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutant 花调节基因HUA2的突变使拟南芥海藻糖6-磷酸合成酶1 （tps1）突变体恢复开花

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-06-05 DOI: 10.1093/plphys/kiaf225

Liping Zeng, Vasiliki Zacharaki, Sam W van Es, Yanwei Wang, Markus Schmid

{"title":"Mutations in the floral regulator gene HUA2 restore flowering to the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutant","authors":"Liping Zeng, Vasiliki Zacharaki, Sam W van Es, Yanwei Wang, Markus Schmid","doi":"10.1093/plphys/kiaf225","DOIUrl":"https://doi.org/10.1093/plphys/kiaf225","url":null,"abstract":"Plant growth and development are regulated by many factors, including carbohydrate availability and signaling. Trehalose 6-phosphate (T6P), which is synthesized by TREHALOSE-6-PHOSPHATE SYNTHASE 1 (TPS1), is positively associated with and functions as a signal that informs the cell about the carbohydrate status. Mutations in TPS1 negatively affect the growth and development of Arabidopsis (Arabidopsis thaliana), and complete loss-of-function alleles are embryo-lethal, which can be overcome using inducible expression of TPS1 (GVG::TPS1) during embryogenesis. Using EMS mutagenesis in combination with genome re-sequencing, we have identified several alleles in the floral regulator gene HUA2 that restore flowering in tps1-2 GVG::TPS1. Genetic analyses using a HUA2 T-DNA insertion allele, hua2-4, confirmed this finding. RNA-seq analyses demonstrated that hua2-4 has widespread effects on the tps1-2 GVG::TPS1 transcriptome, including key genes and pathways involved in regulating flowering. Higher order mutants combining tps1-2 GVG::TPS1 and hua2-4 with alleles in the key flowering time regulators FLOWERING LOCUS T (FT), SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1), and FLOWERING LOCUS C (FLC) were constructed to analyze the role of HUA2 during floral transition in tps1-2 in more detail. Our findings demonstrate that loss of HUA2 can restore flowering in tps1-2 GVG::TPS1, in part through activation of FT, with contributions from the upstream regulators SOC1 and FLC. Interestingly, we found that mutation of FLC is sufficient to induce flowering in tps1-2 GVG::TPS1. Furthermore, we observed that mutations in HUA2 modulate carbohydrate signaling and that this regulation might contribute to flowering in hua2-4 tps1-2 GVG::TPS1.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"70 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144228629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0