乙烯响应因子ERF1A调控uv - c诱导桃果延迟成熟。

IF 6.9 1区生物学 Q1 PLANT SCIENCES

Plant Physiology Pub Date : 2025-09-22 DOI:10.1093/plphys/kiaf409

Elpida Nasiopoulou,Michail Michailidis,Christina Skodra,Ioannis-Dimosthenis S Adamakis,Martina Samiotaki,Georgia Tanou,Christos Bazakos,Athanasios Dalakouras,Athanassios Molassiotis

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引用次数: 0

摘要

紫外线- c （UV-C）照射延迟水果成熟，但潜在的机制尚不清楚。通过对桃果果皮和果肉的分析，研究了桃果对UV-C的组织特异性反应。UV-C处理改变了中央代谢，促进了花青素的积累和着色，并延迟了成熟，这可以通过减少果实软化和水分损失来证明。然而，UV-C增强乙烯产生并上调乙烯相关基因，表明乙烯反应的重新配置。在uv - c应答基因中，aptala2 /乙烯应答因子（AP2/ERF）转录因子家族受影响最大，其中乙烯应答因子1A （ERF1A）在处理后的果皮中表现出最强的诱导作用，表明其在uv - c诱导的成熟延迟中起关键整合作用。UV-C增加了果皮中DNA 5-甲基胞嘧啶（5mC）和RNA n6 -甲基腺苷（m6A）的水平，但没有改变胞嘧啶甲基化或引起ERF1A突变。通过RNA干扰沉默ERF1A证实了它调节乙烯产生、软化和成熟相关代谢产物。免疫定位揭示了erf1a沉默果实细胞壁成分的变化，包括阿拉伯半乳聚糖、果胶和木葡聚糖。erf1a沉默的果皮表现出生长素和水杨酸水平升高，脱落酸含量降低。此外，ERF1A抑制改变了糖、酚类化合物和挥发物的生物合成。我们在ERF1A沉默的果皮中发现了广泛的蛋白质组重编程，并鉴定了可能含有ERF1A结合位点或与硬度、乙烯信号、植物激素代谢和颜色相关的ERF1A靶基因。值得注意的是，羧酸酯酶11 （PpCXE11）、羧酸酯酶13 （PpCXE13）和水杨酸结合蛋白2 （PpSABP2）成为潜在的ERF1A靶点。这些发现表明ERF1A是通过调节乙烯信号和下游成熟途径介导uv - c诱导的成熟延迟的中央调节剂。

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The ethylene response factor ERF1A regulates UV-C-induced delayed ripening in peach fruit.

Ultraviolet-C (UV-C) irradiation delays fruit ripening, yet the underlying mechanisms remain unclear. We investigated tissue-specific responses of peach fruit (Prunus persica L. Batsch) to UV-C by analyzing the peel and flesh separately. UV-C treatment altered central metabolism, promoted anthocyanin accumulation and coloration, and delayed ripening, as evidenced by reduced fruit softening and water loss. However, UV-C enhanced ethylene production and upregulated ethylene-related genes, indicating a reconfiguration of the ethylene response. Among UV-C-responsive genes, the APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factor family was most affected, with Ethylene Response Factor 1A (ERF1A) showing the strongest induction in the treated peel, suggesting its role as a key integrator of the UV-C-induced ripening delay. UV-C increased the levels of DNA 5-methylcytosine (5mC) and RNA N6-methyladenosine (m6A) in the peel, without altering cytosine methylation or causing mutations in ERF1A. Silencing ERF1A via RNA interference confirmed that it regulates ethylene production, softening, and ripening-associated metabolites. Immunolocalization revealed changes in the cell wall components of ERF1A-silenced fruit, including arabinogalactan, pectin, and xyloglucan. ERF1A-silenced peels exhibited elevated auxin and salicylic acid levels and reduced abscisic acid content. Additionally, ERF1A suppression altered the biosynthesis of sugars, phenolic compounds, and volatiles. We found extensive proteome reprogramming in ERF1A-silenced peels and identified putative ERF1A target genes that either contain ERF1A-binding sites or are associated with firmness, ethylene signaling, phytohormone metabolism, and color. Notably, Carboxylesterase 11 (PpCXE11), Carboxylesterase 13 (PpCXE13), and Salicylic acid-binding protein 2 (PpSABP2) emerged as potential ERF1A targets. These findings identify ERF1A as a central regulator mediating UV-C-induced ripening delay through modulation of ethylene signaling and downstream ripening pathways.

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来源期刊

Plant Physiology 生物-植物科学

CiteScore

12.20

自引率

5.40%

发文量

535

审稿时长

2.3 months

期刊介绍： Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.