Plant Physiology最新文献_第8页

Role of polar localization of the silicon transporter OsLsi1 in metalloid uptake by rice roots 硅转运体OsLsi1的极性定位在水稻根系吸收类金属中的作用

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-09 DOI: 10.1093/plphys/kiaf196

Noriyuki Konishi, Namiki Mitani-Ueno, Jian Feng Ma

{"title":"Role of polar localization of the silicon transporter OsLsi1 in metalloid uptake by rice roots","authors":"Noriyuki Konishi, Namiki Mitani-Ueno, Jian Feng Ma","doi":"10.1093/plphys/kiaf196","DOIUrl":"https://doi.org/10.1093/plphys/kiaf196","url":null,"abstract":"Low silicon (Si) rice 1 (OsLsi1) is a key transporter mediating Si uptake in rice (Oryza sativa). It is polarly localized at the distal side of the root exodermis and endodermis. Although OsLsi1 is also permeable to other metalloids, such as boron (B), germanium (Ge), arsenic (As), antimony (Sb), and selenium (Se), the role of its polar localization in the uptake of these metalloids remains unclear. In this study, we investigated the role of OsLsi1 polar localization in metalloid uptake by examining transgenic rice plants expressing polarly or non-polarly localized OsLsi1 variants. Loss of OsLsi1 polar localization resulted in decreased accumulation of Ge, B, and As in shoots but increased Sb accumulation, while Se accumulation remained unaffected under normal conditions. Experiments with varying B concentrations revealed that B uptake is significantly lower at low B concentrations (0.3–3 μM) but higher at high B concentrations (300 μM) in plants expressing non-polarly localized OsLsi1, despite the similar B permeability of both OsLsi1 variants in Xenopus oocytes and their comparable protein abundance in roots. Additionally, the loss of OsLsi1 polarity did not affect the abundance, localization, or high B-induced degradation of the borate transporter 1 (OsBOR1), an efflux transporter that cooperates with OsLsi1 for B uptake. Taken together, our findings demonstrate that the polar localization of OsLsi1 plays a critical role in regulating metalloid uptake, depending on the presence or absence of efflux transporters cooperating with OsLsi1.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"38 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Open and shut: apoplastic water availability dominates stomatal immunity in determining disease resistance. 开闭：外体水分有效性在决定抗病性方面主导气孔免疫。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-09 DOI: 10.1093/plphys/kiaf176

Josephine H R Maidment,Bo Xu

引用次数: 0

The conserved splicing factor PRP21 regulates the abscisic acid response and seed germination in Arabidopsis 保守剪接因子PRP21调控拟南芥的脱落酸响应和种子萌发

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-09 DOI: 10.1093/plphys/kiaf189

Hao-Ran Zhou, Feng Qian, Jin-Lu Du, Juncheng Lin, Zhen-Zhen Liu, Dan-Yang Yuan, Huan-Wei Huang, Tao Cai, Lin Li, She Chen, Qingshun Quinn Li, Xin-Jian He

{"title":"The conserved splicing factor PRP21 regulates the abscisic acid response and seed germination in Arabidopsis","authors":"Hao-Ran Zhou, Feng Qian, Jin-Lu Du, Juncheng Lin, Zhen-Zhen Liu, Dan-Yang Yuan, Huan-Wei Huang, Tao Cai, Lin Li, She Chen, Qingshun Quinn Li, Xin-Jian He","doi":"10.1093/plphys/kiaf189","DOIUrl":"https://doi.org/10.1093/plphys/kiaf189","url":null,"abstract":"Seed germination is a critical developmental stage in the lifecycle of plants, and its regulation is essential for ensuring crop productivity, particularly under adverse environmental conditions. Here, we find that the Arabidopsis thaliana Pre-mRNA PROCESSING FACTOR 21 (PRP21) is crucial for regulating the abscisic acid (ABA) response and seed germination. Our RNA deep sequencing and poly(A) tag sequencing analyses reveal that PRP21 is involved in pre-mRNA splicing, genome-wide gene expression, and mRNA 3' end processing, highlighting its multifunctional role in gene regulation. Furthermore, PRP21 interacts with various splicing factors and small nuclear ribonucleoproteins, confirming its involvement in spliceosome assembly. Additionally, we demonstrate that PRP21 negatively regulates the expression of ABA-responsive genes, such as ABI3, ABI5, EM1, and EM6, thereby modulating ABA response and seed germination. Our findings underscore the importance of PRP21 in coordinating transcriptional and post-transcriptional processes and provide insights into the molecular mechanisms underlying seed germination, potentially guiding crop improvement for stress tolerance.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"14 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor ESR2/DRNL/BOL orchestrates cytokinin dynamics leading to developmental reprogramming and green callus formation 转录因子ESR2/DRNL/BOL协调细胞分裂素动力学，导致发育重编程和绿色愈伤组织形成

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-09 DOI: 10.1093/plphys/kiaf182

Yolanda Durán-Medina, David Díaz-Ramírez, Humberto Herrera-Ubaldo, Maurizio Di Marzo, J Erik Cruz-Valderrama, Herenia Guerrero-Largo, Beatriz E Ruiz-Cortés, Andrea Gómez Felipe, J Irepan Reyes-Olalde, Lucia Colombo, Ondrej Novak, Stefan de Folter, Nayelli Marsch-Martínez

{"title":"The transcription factor ESR2/DRNL/BOL orchestrates cytokinin dynamics leading to developmental reprogramming and green callus formation","authors":"Yolanda Durán-Medina, David Díaz-Ramírez, Humberto Herrera-Ubaldo, Maurizio Di Marzo, J Erik Cruz-Valderrama, Herenia Guerrero-Largo, Beatriz E Ruiz-Cortés, Andrea Gómez Felipe, J Irepan Reyes-Olalde, Lucia Colombo, Ondrej Novak, Stefan de Folter, Nayelli Marsch-Martínez","doi":"10.1093/plphys/kiaf182","DOIUrl":"https://doi.org/10.1093/plphys/kiaf182","url":null,"abstract":"Callus formation and shoot regeneration are naturally triggered by stress and damage to the plant. They are also key components of tissue culture, which is crucial for gene editing, transformation, propagation, and other technologies in many species. Thus, the study of callus formation and shoot regeneration provides valuable insights into plant development. The transcription factor ENHANCER OF SHOOT REGENERATION 2/DORNROSCHEN-LIKE/BOLITA/SUPPRESSOR OF PHYTOCHROME B-4 2 (ESR2/DRNL/BOL/SOB2) promotes green callus formation in roots as well as shoot regeneration when overactive, and the phythormone cytokinin plays a prominent role in both processes. Yet, the role of ESR2 in the cytokinin pathway has not been previously described in Arabidopsis (Arabidopsis thaliana). We found that cytokinin content and the expression of the cytokinin biosynthesis gene ISOPENTENYLTRANSFERASE 5 (IPT5) are greater in plants with high ESR2 activity. ESR2 also regulates the cytokinin signaling repressor ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 6 (AHP6), and surprisingly, ESR2-stimulated green callus formation requires IPT5 and AHP6. Therefore, ESR2 promotes both cytokinin biosynthesis and the inhibition of cytokinin signaling and, paradoxically, requires a combination of these two effects for green callus induction. These findings provide a foundation to better understand the processes involved in tissue reprogramming towards callus formation and the role of ESR2 in callus formation and shoot regeneration.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"118 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The quantitative effect of seed production triggers the end of flowering in tomato 种子生产的数量效应触发了番茄的开花结束

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-09 DOI: 10.1093/plphys/kiaf195

María Jesús López-Martín, Cristina Ferrándiz, Concepción Gómez-Mena

{"title":"The quantitative effect of seed production triggers the end of flowering in tomato","authors":"María Jesús López-Martín, Cristina Ferrándiz, Concepción Gómez-Mena","doi":"10.1093/plphys/kiaf195","DOIUrl":"https://doi.org/10.1093/plphys/kiaf195","url":null,"abstract":"Flowering plants adjust their reproductive period to ensure reproductive success. This involves a tight control of both flower initiation and the termination of the flowering period to optimize resource allocation for seed production. The end of flowering is marked by the cessation of flower production by the inflorescence meristems, which enter a dormant-like state known as proliferative arrest. This process has been mainly studied in Arabidopsis (Arabidopsis thaliana) at the physiological, genetic, and molecular levels but remains to be characterized in other species, which could provide general mechanisms and the groundwork for designing biotechnological strategies aimed at controlling the duration of fruit/seed production. Solanum lycopersicum (tomato) is an excellent model for this goal because of its economic importance and the marked differences in plant architecture, meristem organization, and fruit development. By comparing plants producing fertile and parthenocarpic seedless fruits, we have determined that proliferative arrest in tomato is a reversible process triggered by seed formation. We have identified the seeds as the likely source of signals that instruct the meristems to arrest in a coordinated and quantitative manner. The presence of auxin and abscisic acid in exudates from fertile but not from parthenocarpic fruits and the effect of treating seedless fruits with exogenous auxin on proliferative arrest support a major role of these phytohormones in the communication between seeds and meristems. Our work supports the conservation of factors controlling proliferative arrest in flowering plants while providing insights into the regulation of this process.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"118 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Symmetry-adjusted cryo-EM analysis unveils the detailed linker protein CsoS2 interactions within the α-carboxysome shell 对称性调整的低温电镜分析揭示了α-羧基体外壳内连接蛋白CsoS2的详细相互作用

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-09 DOI: 10.1093/plphys/kiaf165

Jianxun Li, Tianpei Li, Saimeng Wang, Yu-Zhong Zhang, Lu-Ning Liu, Peng Wang

引用次数: 0

Clathrin-mediated trafficking regulates copper tolerance by modulating the localization of HEAVY METAL ATPase 5 in Arabidopsis root cells 网格蛋白介导的转运通过调节拟南芥根细胞中重金属atp酶5的定位来调节铜耐受性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-09 DOI: 10.1093/plphys/kiaf183

Liufan Wang, Mei Xu, Yonghua Shao, Guochao Zhang, Yuling Ran, Hongqian Lu, Jiaqi Ma, Jieming Jiang, Xifeng Chen, Xu Yan, Jianxin Shou, Chao Wang

{"title":"Clathrin-mediated trafficking regulates copper tolerance by modulating the localization of HEAVY METAL ATPase 5 in Arabidopsis root cells","authors":"Liufan Wang, Mei Xu, Yonghua Shao, Guochao Zhang, Yuling Ran, Hongqian Lu, Jiaqi Ma, Jieming Jiang, Xifeng Chen, Xu Yan, Jianxin Shou, Chao Wang","doi":"10.1093/plphys/kiaf183","DOIUrl":"https://doi.org/10.1093/plphys/kiaf183","url":null,"abstract":"Plant clathrin and its adaptor protein complexes—adaptor protein complex-1 (AP-1) at the trans-Golgi network/early endosome (TGN/EE) and the adaptor protein complex-2 (AP-2) at the plasma membrane (PM)—function in clathrin-mediated trafficking (CMT). This study reports the role of CMT in regulating copper (Cu) tolerance in plants. We found that high concentrations of exogenous Cu treatment increase the abundance of clathrin and adaptor protein complexes at the TGN/EE and/or the PM. We further found that a CMT-deficient mutants ap2μ2, clc2 clc3 exhibits hypersensitivity to Cu stress, similar to a mutant lacking the Cu transporter HEAVY METAL ATPase 5 (HMA5). As previously reported, HMA5 relocates from the endoplasmic reticulum (ER) to the PM on the soil side, where it excretes excess Cu from the root cell, which is crucial for Cu tolerance. Our protein interaction assays showed that the AP-1 and AP-2 σ subunits depend on the YXXΦ sorting motif of HMA5 for recognition. Defective AP-1 hinders HMA5 translocation to the PM after its transfer from the ER to the TGN/EE following Cu stress, while impaired AP-2 function inhibits HMA5 endocytosis at the PM. These results demonstrate that CMT mediates the endocytic recycling of HMA5 between the TGN/EE and the PM, thereby regulating Cu efflux from root cells. Our findings highlight a function of CMT in maintaining Cu homeostasis.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"39 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The pentatricopeptide repeat protein PPR767 modulates plant architecture and drought resistance in rice. 五肽重复蛋白PPR767调控水稻植株结构和抗旱性。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-07 DOI: 10.1093/plphys/kiaf187

Leilei Peng,Haijun Xiao,Yanghong Xu,Zhihao Huang,Xuan Yang,Chen Lv,Linghui Huang,Jun Hu

{"title":"The pentatricopeptide repeat protein PPR767 modulates plant architecture and drought resistance in rice.","authors":"Leilei Peng,Haijun Xiao,Yanghong Xu,Zhihao Huang,Xuan Yang,Chen Lv,Linghui Huang,Jun Hu","doi":"10.1093/plphys/kiaf187","DOIUrl":"https://doi.org/10.1093/plphys/kiaf187","url":null,"abstract":"The RNA-binding proteins (RBPs) encoded by the nucleus are essential for RNA metabolism in eukaryotes. Pentatricopeptide repeat (PPR) proteins, a large subset of RBPs, are essential for plant development and reproduction by participating in organellar RNA processing. Here, we identified an E-type PPR protein, PPR767, which functions in mitochondria. Knocking out PPR767 resulted in decreased plant height, thinner stems, shorter and narrower blades, and consequently affected yield traits compared to those of the wild-type. PPR767 primarily participated in the RNA editing of four sites related to NADH dehydrogenase (Nad), including nad1-674, nad3-155, nad3-172, and nad7-317. PPR767 interacted with multiple organellar RNA editing factors (MORFs), including MORF1 and MORF8, suggesting that the editosome in rice (Oryza sativa) is complex. The mutants showed decreased mitochondrial complex Ⅰ activity and compromised mitochondrial structure. Furthermore, mutation of PPR767 influenced rice drought tolerance and the expression levels of genes involved in reactive oxygen species (ROS) accumulation. Therefore, PPR767 is essential for complex Ⅰ activity by properly regulating the RNA editing efficiency of mitochondrial genes and affects drought tolerance by modulating ROS content in rice. Our findings provide valuable insights into the mechanisms by which PPRs fulfil their functions.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the evolutionary association of vitamin E, chlorophyll, and PSII through parasitic plants. 通过寄生植物揭示维生素E、叶绿素和PSII的进化关联。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-07 DOI: 10.1093/plphys/kiaf185

Laia Jené,Sergi Munné-Bosch

{"title":"Unveiling the evolutionary association of vitamin E, chlorophyll, and PSII through parasitic plants.","authors":"Laia Jené,Sergi Munné-Bosch","doi":"10.1093/plphys/kiaf185","DOIUrl":"https://doi.org/10.1093/plphys/kiaf185","url":null,"abstract":"The evolution of parasitic plants has been marked by a progressive relaxation of selective pressures associated with maintaining photosynthesis, resulting in a wide diversity in photosynthetic capacity within this group. In this study, we explored this diversity by examining several hemi- and holoparasitic plants, focusing on photoprotection. Our findings revealed a strongly conserved evolutionary association between vitamin E, PSII activity, and chlorophyll content in parasitic plants, with α-tocopherol consistently being identified as the predominant vitamin E form. To validate the antioxidant and photoprotective role of α-tocopherol in a plant with reduced photosynthetic capacity, we investigated the interaction between the stem holoparasitic plant field dodder (Cuscuta campestris Yunck.), which retains partial PSII activity and low chlorophyll levels, and its host, lentil plant (Lens culinaris Medik). This protective role, essential for controlling lipid peroxidation within chloroplasts, was demonstrated both in planta and in isolated chloroplasts from field dodder exposed to photoinhibitory conditions induced by the synthetic photosensitiser Rose Bengal and light. Notably, our findings highlight the final evolutionary step in the conserved role of vitamin E in photosynthesis and photoprotection as revealed through parasitic plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"56 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143914946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing genomic prediction for complex traits via investigating multiple factors in switchgrass 通过对柳枝稷多因子的研究优化复杂性状的基因组预测

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-05-07 DOI: 10.1093/plphys/kiaf188

Peipei Wang, Fanrui Meng, Christina B Del Azodi, Kenia Segura Abá, Michael D Casler, Shin-Han Shiu

{"title":"Optimizing genomic prediction for complex traits via investigating multiple factors in switchgrass","authors":"Peipei Wang, Fanrui Meng, Christina B Del Azodi, Kenia Segura Abá, Michael D Casler, Shin-Han Shiu","doi":"10.1093/plphys/kiaf188","DOIUrl":"https://doi.org/10.1093/plphys/kiaf188","url":null,"abstract":"Genomic prediction has accelerated breeding processes and provided mechanistic insights into the genetic bases of complex traits. To further optimize genomic prediction, we assess the impact of genome assemblies, genotyping approaches, variant types, allelic complexities, polyploidy levels, and population structures on the prediction of 20 complex traits in switchgrass (Panicum virgatum L.), a perennial biofuel feedstock. Surprisingly, short read-based genome assembly performs comparably to or even better than long read-based assembly. Due to higher gene coverage, exome capture and multi-allelic variants outperform genotyping-by-sequencing and bi-allelic variants, respectively. Tetraploid models show higher prediction accuracy than octoploid models for most traits, likely due to the greater genetic distances among tetraploids. Depending on the trait in question, different types of variants need to be integrated for optimal predictions. Our study provides insights into the factors influencing genomic prediction outcomes, guiding best practices for future studies and for improving agronomic traits in switchgrass and other species through selective breeding.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"63 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143920038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0