Plant Physiology最新文献_第8页

The FaPSMD14-FaEIN3-FaWRKY40 module enhances strawberry resistance to Botrytis cinerea by modulating cytokinin and jasmonic acid biosynthesis. FaPSMD14-FaEIN3-FaWRKY40模块通过调节细胞分裂素和茉莉酸的生物合成来增强草莓对葡萄孢杆菌的抗性。

IF 8.2 1区生物学

Plant Physiology Pub Date : 2026-10-06 DOI: 10.1093/plphys/kiag689

Xiaoying Zhu, Kaiyue Xing, Lihang Zhang, Changxu Duan, Mengmeng Xu, Liguo Feng, Jianqiang Yu, Lixia Sheng

{"title":"The FaPSMD14-FaEIN3-FaWRKY40 module enhances strawberry resistance to Botrytis cinerea by modulating cytokinin and jasmonic acid biosynthesis.","authors":"Xiaoying Zhu, Kaiyue Xing, Lihang Zhang, Changxu Duan, Mengmeng Xu, Liguo Feng, Jianqiang Yu, Lixia Sheng","doi":"10.1093/plphys/kiag689","DOIUrl":"10.1093/plphys/kiag689","url":null,"abstract":"Gray mold, caused by Botrytis cinerea, severely threatens strawberry production worldwide, yet the molecular mechanisms underlying strawberry resistance remain poorly understood. Here, we show that the transcription factor ETHYLENE INSENSITIVE 3 (FaEIN3), a strawberry EIN3/EIL homolog, positively regulates strawberry resistance to B. cinerea by directly activating the transcription of the WRKY transcription factor gene FaWRKY40. FaWRKY40 further induces the cytokinin biosynthesis gene FaLOG1 and the jasmonic acid biosynthesis gene FaAOC4, thereby promoting hormone accumulation and enhancing disease resistance. Moreover, the JAMM-family deubiquitinase FaPSMD14 physically interacts with FaEIN3 in the nucleus and stabilizes FaEIN3, thereby strengthening FaEIN3-mediated activation of FaWRKY40. Our findings reveal a previously uncharacterized defense mechanism in which the FaPSMD14-FaEIN3-FaWRKY40 regulatory cascade promotes strawberry resistance to B. cinerea through coordinated activation of the cytokinin and jasmonic acid pathways.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148983367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of phloem unloading and post-phloem transport of photoassimilates during the rapid elongation of young Dendrocalamus brandisii shoots. 松菖蒲幼芽快速伸长过程中光同化物质的韧皮部卸载和韧皮部后运输机制。

IF 8.2 1区生物学

Plant Physiology Pub Date : 2026-10-06 DOI: 10.1093/plphys/kiag687

Fangwei Zhu, Chongsheng Zhao, Yufang Wu, Jiaxin Liu, Lixia Yu, Hui Zhan, Huijin Fan, Lingfeng Li, Shuyan Lin, Shuguang Wang

{"title":"Mechanisms of phloem unloading and post-phloem transport of photoassimilates during the rapid elongation of young Dendrocalamus brandisii shoots.","authors":"Fangwei Zhu, Chongsheng Zhao, Yufang Wu, Jiaxin Liu, Lixia Yu, Hui Zhan, Huijin Fan, Lingfeng Li, Shuyan Lin, Shuguang Wang","doi":"10.1093/plphys/kiag687","DOIUrl":"10.1093/plphys/kiag687","url":null,"abstract":"Phloem unloading plays a key role in the transport and allocation of photoassimilates. Elucidating the unloading process and metabolic dynamics of photoassimilates in rapidly elongating internodes is crucial for understanding the rapid growth mechanism of bamboo. We systematically investigated the phloem unloading and the post-unloading transport mechanism in internodes of young Dendrocalamus brandisii culms through integrated anatomical observations, transcriptomic analysis, and gene functional verification. Phloem unloading shifts from coexisting apoplasmic and symplasmic pathways in immature internodes to an exclusively symplasmic mechanism in mature internodes. DbSWEET1a and DbPMT1 were highly expressed in rapidly elongating internodes. Exogenous sucrose application further confirmed their role as key genes by upregulating genes involved in apoplasmic phloem unloading and post-unloading transport in immature internodes. Plasma membrane proteins DbSWEET1a and DbPMT1 mainly localized in phloem companion cells and adjacent parenchyma cells. Yeast complementation assays verified that DbSWEET1a transports sucrose and fructose, while DbPMT1 transports glucose and fructose. SWEET1a and PMT1 proteins participate in apoplasmic phloem unloading and transport in immature internodes, whereas in mature internodes, they facilitate the retrieval of apoplasmic sugars for reintegration into the symplasmic unloading pathway.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148983378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutritional coordination via sulfate transport drives Beauveria bassiana plant endophytism. 通过硫酸盐运输的营养协调驱动球孢白僵菌-植物内生作用。

IF 8.2 1区生物学

Plant Physiology Pub Date : 2026-10-06 DOI: 10.1093/plphys/kiag723

Yunxia Liu, Yutong Long, Yazhuo Li, Xin Zhao, Fanlong Wang, Zhibing Luo, Yongjun Zhang

{"title":"Nutritional coordination via sulfate transport drives Beauveria bassiana plant endophytism.","authors":"Yunxia Liu, Yutong Long, Yazhuo Li, Xin Zhao, Fanlong Wang, Zhibing Luo, Yongjun Zhang","doi":"10.1093/plphys/kiag723","DOIUrl":"10.1093/plphys/kiag723","url":null,"abstract":"The entomopathogenic fungus Beauveria bassiana endophytically colonizes a diverse array of plants including the model, Nicotiana benthamiana, stimulating photosynthesis and growth. During this interaction, sulfate transporter genes are upregulated in both organisms. However, the mechanisms mediating this potential mutualistic sulfate exchange remain elusive. Here, we demonstrate that the 3 activated fungal sulfate transporters synergistically facilitated sulfate utilization and promoted fungal colonization of the plant. Among these, the high-affinity sulfate transporter BbSulTR1 plays a primary role. Disruption of any single fungal sulfate transporter genes decreased B. bassiana colonization of plant, consequently decreasing plant sulfur accumulation and growth promotion. Similarly, single interference of the 3 activated plant sulfate transporter genes severely attenuated plant sulfate acquisition and biomass, subsequently restricting fungal colonization. Mechanistically, the endophytic fungus actively acquires sulfate from the host to enhance its own organic sulfur synthesis. This depletion of host sulfate and organic sulfur pools triggers the plant's low-sulfur response, upregulating host sulfate transporters to accelerate external sulfate uptake. Notably, loss of BbSulTR1 significantly abolished these reciprocal dynamics. Our findings reveal that fungal sulfate acquisition is critical for fungal endophytic colonization and that the mutualistic interaction integrates a feedback mechanism that stimulates host sulfur assimilation pathways ultimately contributing to plant growth. This study elucidates a novel nutritional coordination mechanism underpinning fungal plant endophytism.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genome of Bangia fuscopurpurea illustrates early establishment of multicellularity and intertidal adaptation in red algae. 红藻多细胞生物的早期建立和潮间带适应性研究

IF 8.2 1区生物学

Plant Physiology Pub Date : 2026-10-06 DOI: 10.1093/plphys/kiag695

Yuanyuan Hui, Dexin Lyu, Tianjun Cao, Yinyin Deng, Li-En Yang, Huihui Cheng, Hanyu Liang, Na Huang, Zihan Ge, Jia Liu, Libao Zheng, Fei Zhou, Pengfu Li, Jianyi Zhu, Qinqin Lu, Yunyun Zhuang, Charles Yarish, Zonggen Shen, Jianfeng Niu, Guangce Wang, Weizhou Chen, Shan Lu, Kui Li

{"title":"The genome of Bangia fuscopurpurea illustrates early establishment of multicellularity and intertidal adaptation in red algae.","authors":"Yuanyuan Hui, Dexin Lyu, Tianjun Cao, Yinyin Deng, Li-En Yang, Huihui Cheng, Hanyu Liang, Na Huang, Zihan Ge, Jia Liu, Libao Zheng, Fei Zhou, Pengfu Li, Jianyi Zhu, Qinqin Lu, Yunyun Zhuang, Charles Yarish, Zonggen Shen, Jianfeng Niu, Guangce Wang, Weizhou Chen, Shan Lu, Kui Li","doi":"10.1093/plphys/kiag695","DOIUrl":"10.1093/plphys/kiag695","url":null,"abstract":"The billion-year-old fossil red alga Bangiomorpha pubescens represents the oldest taxonomically resolved multicellular eukaryote, morphologically indistinguishable from the extant intertidal alga Bangia fuscopurpurea. Here, we report a chromosome-level 107.55 Mb genome assembly of B. fuscopurpurea. Phylogenomic analysis confirms its placement as the most basal multicellular red algal lineage, diverging ∼973.40 million years ago. The genome reveals lineage-specific expansion of Osvaldo-type LTR retrotransposons that influence generation-specific gene expression. Metabolically, B. fuscopurpurea relies exclusively on the methylerythritol phosphate pathway for terpenoid biosynthesis, lacking the mevalonate pathway. Genomic analysis reveals the presence of core abscisic acid biosynthetic genes alongside some components of other hormone pathways, suggesting that fundamental phytohormone biosynthetic capacity was established in early red algal lineages. Multi-omics profiling reveals pronounced transcriptional and epigenetic differences between haploid gametophytes and diploid sporophytes, with distinct generation-specific DNA methylation patterns (5mC and 6mA). Time-series transcriptomic analyses identified 2,320 rhythmically expressed genes exhibiting diurnal patterns aligned with intertidal environmental fluctuations, including reactive oxygen species detoxification genes peaking at dusk and photoprotective genes at dawn. These findings illuminate the genomic innovations underlying early multicellular evolution, life cycle regulation, and intertidal adaptation in ancient red algae, establishing B. fuscopurpurea as a critical reference for understanding photosynthetic eukaryote diversification.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149002438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lignin structural changes and high p-coumaroylation in incipient lignification in moso bamboo. 毛竹木质化初期木质素结构变化和高对香豆素酰化。

IF 8.2 1区生物学

Plant Physiology Pub Date : 2026-10-06 DOI: 10.1093/plphys/kiag407

Noriaki Munekata, Steven D Karlen, Arata Yoshinaga, Taku Tsuyama, Rebecca A Smith, Fabienne Guillon, Tatsuya Awano, John Ralph, Junji Sugiyama

{"title":"Lignin structural changes and high p-coumaroylation in incipient lignification in moso bamboo.","authors":"Noriaki Munekata, Steven D Karlen, Arata Yoshinaga, Taku Tsuyama, Rebecca A Smith, Fabienne Guillon, Tatsuya Awano, John Ralph, Junji Sugiyama","doi":"10.1093/plphys/kiag407","DOIUrl":"10.1093/plphys/kiag407","url":null,"abstract":"Lignification is a crucial process for strengthening plant tissues, facilitating water transport, and providing defense against pathogens. In the Poaceae family, p-hydroxycinnamic acids are commonly incorporated into lignin, with acylation by p-coumarate (pCA) occurring during lignification. In this study, we performed DFRC and 2D HSQC-NMR analyses to investigate changes in lignin substructures and the degree of lignin pCA-acylation throughout bamboo stem development. Furthermore, immunohistochemical analysis was conducted to elucidate the spatial distribution of lignin substructures within different cell types. Our results revealed that, in young tissues, β-O-4-linked lignin units are predominantly derived from monolignol-pCA conjugates, specifically coniferyl- and sinapyl-pCA. Both lignin structure and the pattern of pCA acylation varied depending on the stage of cell wall formation and the cell type, particularly between vascular fiber cells and parenchyma cells. Based on our results, moso bamboo culms exhibit a distinctive feature during incipient lignification, in which monolignols are predominantly acylated with pCA. This feature has not been reported in other grasses, suggesting that extensive p-coumaroylation of monolignols plays an important role in the rapid elongation of bamboo culms.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148278513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophosphorylation at the C-terminus of the DMI3 calmodulin-binding domain dismantles CaM-DMI3-IPD3 to initiate root nodulation. DMI3钙调素结合域c端的自磷酸化会破坏CaM-DMI3-IPD3，从而启动根结。

IF 8.2 1区生物学

Plant Physiology Pub Date : 2026-10-06 DOI: 10.1093/plphys/kiag347

Yiting Zhou, Yuchen Qian, Yiang Wang, Xiang Li, Yingying Huang, Liqiang Dai, Lihui Duan, Qian Guo, Kedi Jiang, Shang Wang, Jinbo Shen, Liqun Du, Erxu Pi

{"title":"Autophosphorylation at the C-terminus of the DMI3 calmodulin-binding domain dismantles CaM-DMI3-IPD3 to initiate root nodulation.","authors":"Yiting Zhou, Yuchen Qian, Yiang Wang, Xiang Li, Yingying Huang, Liqiang Dai, Lihui Duan, Qian Guo, Kedi Jiang, Shang Wang, Jinbo Shen, Liqun Du, Erxu Pi","doi":"10.1093/plphys/kiag347","DOIUrl":"10.1093/plphys/kiag347","url":null,"abstract":"The calcium/calmodulin-dependent protein kinase (CCaMK) DMI3 (Doesn't Make Infections 3) decodes rhizobia-induced Ca2+ signals through phosphorylation of its interacting partner, IPD3, thereby initiating root nodule symbiosis (RNS). However, the precise mechanism by which DMI3 activates this pathway remains unclear. Here, we show that phosphorylation of a triplet motif (S343S344T345) located at the C-terminus of the calmodulin-binding domain acts as a molecular switch. We identified T345 as a phosphorylation site that exhibits regulatory functions similar to those of S343 and S344. Phosphomimic mutations at any single residue of the 3 sites blocked DMI3-dependent nodulation, indicating that the kinase must initially remain nonphosphorylated at these positions. Conversely, simultaneous phosphorylation of at least 2 residues was required for symbiotic activity, revealing a transition from a phosphorylation-free state to a hyperphosphorylated status (simultaneous phosphorylation of at least 2 residues) at these sites. Phosphomimic mutations within the triplet enhanced DMI3 autophosphorylation (auto-P) but severely impaired its interaction with Ca2+/CaM and IPD3. In addition, the physical interaction between DMI3 and IPD3 suppressed IPD3-triggered MtNIN transcription and nodulation, indicating that DMI3 also acts as a negative regulator by sequestering activated IPD3. Thus, we propose a model in which repeated auto-P at the triplet contributes to RNS activation by dissociating the CaM-DMI3-IPD3 complex, thereby releasing phosphorylated and activated IPD3 to initiate the downstream transcriptional cascade. Our findings uncover a dual role for DMI3-as both a kinase and a scaffold-and clarify how auto-P within the triplet motif licenses IPD3 to trigger RNS.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":8.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148205630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Turn the light on! Using algae to learn how chloroplasts are built. 把灯打开！利用藻类了解叶绿体是如何形成的。

IF 8.2 1区生物学

Plant Physiology Pub Date : 2026-10-06 DOI: 10.1093/plphys/kiag694

Mireia Uranga

引用次数: 0

The AcMYB15-AcWRKY24 module coordinates lignin and salicylic acid accumulation during kiwifruit resistance to bacterial canker AcMYB15-AcWRKY24模块在猕猴桃对细菌溃疡病的抗性过程中协调木质素和水杨酸的积累

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-10-05 DOI: 10.1093/plphys/kiag737

Shunyuan Wu,Wenli Yue,Wanjun Chai,Jilong Cheng,Ge Guo,Dongheng Hui,Lili Huang,Gongyou Chen,Xueren Yin,Yue Huang,Pu Liu

{"title":"The AcMYB15-AcWRKY24 module coordinates lignin and salicylic acid accumulation during kiwifruit resistance to bacterial canker","authors":"Shunyuan Wu,Wenli Yue,Wanjun Chai,Jilong Cheng,Ge Guo,Dongheng Hui,Lili Huang,Gongyou Chen,Xueren Yin,Yue Huang,Pu Liu","doi":"10.1093/plphys/kiag737","DOIUrl":"https://doi.org/10.1093/plphys/kiag737","url":null,"abstract":"Abstract Plants coordinate phenylpropanoid metabolism across different tissues to defend against pathogens, but the underlying transcriptional mechanisms remain unclear. Here, we identify the kiwifruit transcription factor AcMYB15 as a regulator that lignin deposition and salicylic acid (SA) accumulation, conferring bacterial canker resistance in kiwifruit through a tissue-specific regulatory module during Pseudomonas syringae pv. actinidiae (Psa) infection. In leaves, AcMYB15 directly binds to the CAACCC motif in the AcPAL1 promoter to activate its transcription, promoting localized lignin accumulation. In stems, which are the primary conduits for systemic Psa colonization, AcMYB15 forms a protein complex with the stem-enriched transcription factor AcWRKY24. Formation of the AcMYB15-AcWRKY24 protein complex enhanced transcriptional activation of the stem-prevalent AcPAL2 by strengthening its DNA-binding preferences for the CAACCA motif. Functional characterization indicates that AcPAL1 and AcPAL2 exert distinct defensive roles: AcPAL1 overexpression primarily leads to increased lignin deposition, whereas AcPAL2 overexpression predominantly enhances SA accumulation. Therefore, the AcMYB15-AcWRKY24 synergistic module provides a coordinated defense mechanism in the stem by simultaneously promoting lignin deposition and SA accumulation, thereby conferring enhanced resistance to bacterial canker. Our study uncovers a two-tiered transcriptional mechanism that coordinately regulates the tissue-specific accumulation of lignin and SA during Psa infection. These findings provide a solid theoretical basis and potential genetic resources for the genetic improvement and molecular breeding of disease-resistant fruit crops.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"37 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenomic insights into plant terrestrialization: from streptophyte algae to the rise of land plants 植物陆地化的系统基因组研究：从链藻到陆生植物的兴起

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-10-05 DOI: 10.1093/plphys/kiag738

Xiaoya Ma,Yuanhao Li,Zhiping Yang,Yuxuan Zhao,Bojian Zhong

引用次数: 0

The dual role of Leptochloa chinensis LcCYP74A33 confers tripyrasulfone resistance via direct metabolism and JA-mediated antioxidant defense Leptochloa chinensis LcCYP74A33的双重作用通过直接代谢和ja介导的抗氧化防御赋予了三嘧磺酮抗性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-10-03 DOI: 10.1093/plphys/kiag736

Jinhui Li,Junzhi Wang,Jiahao Lun,Lianyang Bai,Lang Pan

引用次数: 0