Plant Physiology最新文献_第8页

Maize stigmas react differently to self- and cross-pollination and fungal invasion. 玉米柱头对自花授粉、异花传粉和真菌入侵的反应不同。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-02 DOI: 10.1093/plphys/kiae536

Kevin Begcy, Mariana Mondragón-Palomino, Liang-Zi Zhou, Patricia-Lena Seitz, Mihaela-Luiza Márton, Thomas Dresselhaus

{"title":"Maize stigmas react differently to self- and cross-pollination and fungal invasion.","authors":"Kevin Begcy, Mariana Mondragón-Palomino, Liang-Zi Zhou, Patricia-Lena Seitz, Mihaela-Luiza Márton, Thomas Dresselhaus","doi":"10.1093/plphys/kiae536","DOIUrl":"10.1093/plphys/kiae536","url":null,"abstract":"During sexual reproduction in flowering plants, tip-growing pollen tubes travel from the stigma inside the maternal tissues of the pistil toward ovules. In maize (Zea mays L.), the stigma is highly elongated, forming thread-like strands known as silks. Only compatible pollen tubes successfully penetrate and grow through the transmitting tract of the silk to reach the ovules. Like pollen, fungal spores germinate at the surface of silks and generate tube-like structures (hyphae) penetrating silk tissue. To elucidate commonalities and differences between silk responses to these distinctive invading cells, we compared growth behavior of the various invaders as well as the silk transcriptome after self-pollination, cross-pollination, and infection using 2 different fungi. We report that self-pollination triggers mainly senescence genes, whereas incompatible pollen from Tripsacum dactyloides leads to downregulation of rehydration, microtubule, and cell wall-related genes, explaining the slower pollen tube growth and arrest. Invasion by the ascomycete Fusarium graminearum triggers numerous defense responses including the activation of monolignol biosynthesis and NAC as well as WRKY transcription factor genes, whereas responses to the basidiomycete Ustilago maydis are generally much weaker. We present evidence that incompatible pollination and fungal infection trigger transcriptional reprograming of maize silks cell wall. Pathogen invasion also activates the phytoalexin biosynthesis pathway.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":"3071-3090"},"PeriodicalIF":6.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11638485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Coat protein of cassava common mosaic virus targets RAV1 and RAV2 transcription factors to subvert immunity in cassava.

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-29 DOI: 10.1093/plphys/kiae570

引用次数: 0

The transcription factor GhMYB4 represses lipid transfer and sucrose transporter genes and inhibits fiber cell elongation in cotton. 转录因子 GhMYB4 可抑制棉花中的脂质转移基因和蔗糖转运体基因，并抑制纤维细胞的伸长。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-28 DOI: 10.1093/plphys/kiae637

Yujia Duan, Xiaoguang Shang, Ruoxue Wu, Yujia Yu, Qingfei He, Ruiping Tian, Weixi Li, Guozhong Zhu, Wangzhen Guo

{"title":"The transcription factor GhMYB4 represses lipid transfer and sucrose transporter genes and inhibits fiber cell elongation in cotton.","authors":"Yujia Duan, Xiaoguang Shang, Ruoxue Wu, Yujia Yu, Qingfei He, Ruiping Tian, Weixi Li, Guozhong Zhu, Wangzhen Guo","doi":"10.1093/plphys/kiae637","DOIUrl":"https://doi.org/10.1093/plphys/kiae637","url":null,"abstract":"Cotton (Gossypium hirsutum) fiber is a highly elongated single cell with a thickened cell wall. MYB transcription factors are important regulators of plant cell elongation; however, the molecular mechanism involved in regulating fiber elongation remains to be explored. Here, we present evidence that the R2R3-MYB transcription factor GhMYB4 negatively regulates cotton fiber cell elongation by suppressing the expression of two crucial genes previously reported to affect fiber development: lipid transfer protein 4 (GhLTP4) and sucrose transporter 12 (GhSWEET12). GhMYB4 is preferentially expressed in elongating fiber cells. Knockdown of GhMYB4 in cotton results in longer fiber cells, whereas overexpression of GhMYB4 in Arabidopsis leads to reduced plant height and root length. Transcriptomic and lipidomic analyses revealed that GhMYB4 is involved in coordinating three interconnected biological processes, namely lipid content regulation, auxin signaling, and sugar metabolism. Additionally, we showed that GhMYB4 inhibits the expression of GhLTP4 and GhSWEET12 by binding to the MYB cis-element (TTTAGTG) in their respective promoters. Interestingly, bHLH transcription factor 105 (GhbHLH105) and MYB transcription factor 212 (GhMYB212) counteract the inhibitory effects of GhMYB4 on the expression of GhLTP4 and GhSWEET12, respectively. These findings provide insights into the complex molecular mechanisms regulating plant cell elongation.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amide conjugates of the jasmonate precursor cis-OPDA regulate its homeostasis during plant stress responses. 茉莉酸前体顺式-OPDA的酰胺共轭物在植物胁迫反应过程中调节其平衡。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-28 DOI: 10.1093/plphys/kiae636

Jitka Široká, Anita Ament, Václav Mik, Tomáš Pospíšil, Michaela Kralová, Chao Zhang, Markéta Pernisová, Michal Karady, Vladimira Nožková, Yuho Nishizato, Takuya Kaji, Rina Saito, Mohamed Htitich, Kristýna Floková, Claus Wasternack, Miroslav Strnad, Minoru Ueda, Ondřej Novák, Federica Brunoni

{"title":"Amide conjugates of the jasmonate precursor cis-OPDA regulate its homeostasis during plant stress responses.","authors":"Jitka Široká, Anita Ament, Václav Mik, Tomáš Pospíšil, Michaela Kralová, Chao Zhang, Markéta Pernisová, Michal Karady, Vladimira Nožková, Yuho Nishizato, Takuya Kaji, Rina Saito, Mohamed Htitich, Kristýna Floková, Claus Wasternack, Miroslav Strnad, Minoru Ueda, Ondřej Novák, Federica Brunoni","doi":"10.1093/plphys/kiae636","DOIUrl":"https://doi.org/10.1093/plphys/kiae636","url":null,"abstract":"Jasmonates (JAs) are a family of oxylipin phytohormones regulating plant development and growth and mediating 'defense versus growth' responses. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA) acts independently of CORONATIVE INSENSITIVE 1 (COI1)-mediated JA signaling in several stress-induced and developmental processes. However, its perception and metabolism are only partially understood. A few years ago, a low abundant isoleucine analog of the biologically active JA-Ile, OPDA-Ile, was detected years ago in wounded leaves of flowering plants, opening up the possibility that conjugation of cis-OPDA to amino acids might be a relevant mechanism for cis-OPDA regulation. Here, we extended the analysis of amino acid conjugates of cis-OPDA and identified naturally occurring OPDA-Val, OPDA-Phe, OPDA-Ala, OPDA-Glu, and OPDA-Asp accumulating in response to biotic and abiotic stress in Arabidopsis (Arabidopsis thaliana). The OPDA-amino acid conjugates displayed cis-OPDA-related plant responses in a JA-Ile-dependent manner. We also showed that the synthesis and hydrolysis of cis-OPDA amino acid conjugates are mediated by members of the amidosynthetase GRETCHEN HAGEN 3 (GH3) and the amidohydrolase INDOLE-3-ACETYL-LEUCINE RESISTANT 1 (ILR1)/ILR1-like (ILL) families. Thus, OPDA amino acid conjugates function in the catabolism or temporary storage of cis-OPDA in stress responses instead of acting as chemical signals per se.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factors PuNAC37/PuWRKY74 and E3 ubiquitin ligase PuRDUF2 inhibit volatile ester synthesis in 'Nanguo' pear. 转录因子 PuNAC37/PuWRKY74 和 E3 泛素连接酶 PuRDUF2 可抑制'南果'梨的挥发性酯合成。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-28 DOI: 10.1093/plphys/kiae635

Nannan Zang, Xiaojing Li, Zhuoran Zhang, Weiting Liu, Liyong Qi, Yueming Yang, Qitian Sun, Zepeng Yin, Aide Wang

{"title":"Transcription factors PuNAC37/PuWRKY74 and E3 ubiquitin ligase PuRDUF2 inhibit volatile ester synthesis in 'Nanguo' pear.","authors":"Nannan Zang, Xiaojing Li, Zhuoran Zhang, Weiting Liu, Liyong Qi, Yueming Yang, Qitian Sun, Zepeng Yin, Aide Wang","doi":"10.1093/plphys/kiae635","DOIUrl":"https://doi.org/10.1093/plphys/kiae635","url":null,"abstract":"As major contributors to flavor in many fruit species, volatile esters are useful for investigating the regulation of the biosynthesis pathways of volatile aroma compounds in plants. Ethylene promotes the biosynthesis of volatile esters during fruit storage while accelerating fruit ripening; thus, the ethylene perception inhibitor 1-Methylcyclopropene (1-MCP) is employed to prolong the shelf life of fruits. However, the mechanisms by which 1-MCP regulates volatiles synthesis remain unclear. In this study, we analyzed the pathway of 1-MCP-mediated volatile ester synthesis in 'Nanguo' pear (Pyrus ussuriensis). 1-MCP significantly decreased volatile ester synthesis during storage. Comparative transcriptome analysis showed that the genes encoding two transcription factors (PuNAC37, PuWRKY74) and a RING-type E3 ubiquitin ligase which have a domain of unknown function (PuRDUF2) were expressed at high levels, whereas ALCOHOL ACYLTRANSFERASE 1 (PuAAT1), encoding an enzyme responsible for volatile ester synthesis, was expressed at low levels in 1-MCP-treated fruit. Moreover, PuNAC37 inhibited the expression of PuWRKY74 via transcriptional regulation, whereas PuNAC37 and PuWRKY74, after directly binding to the promoter of PuAAT1, synergistically inhibited its expression in 1-MCP-treated fruit. In addition, in vitro and in vivo ubiquitination experiments revealed that PuRDUF2 functions as an E3 ubiquitin ligase that ubiquitinates PuAAT1, causing its degradation via the 26S proteasome pathway following 1-MCP treatment. Subsequent PuAAT1 degradation resulted in a reduction of volatile esters during fruit storage. Our findings provide insights into the complex transcriptional regulation of volatile ester formation in 'Nanguo' pears and contribute to future research on AAT protein ubiquitination in other species.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-exposure Impact of Nickel Oxide Nanomaterials and Bacillus subtilis on Soybean Growth and Nitrogen Assimilation Dynamics. 氧化镍纳米材料与枯草芽孢杆菌共暴露对大豆生长和氮同化动力学的影响

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-28 DOI: 10.1093/plphys/kiae638

Kashif Khan, Zhen Wei Li, Rayyan Khan, Shahid Ali, Haseeb Ahmad, Muhammad Ali Shah, Xun Bo Zhou

{"title":"Co-exposure Impact of Nickel Oxide Nanomaterials and Bacillus subtilis on Soybean Growth and Nitrogen Assimilation Dynamics.","authors":"Kashif Khan, Zhen Wei Li, Rayyan Khan, Shahid Ali, Haseeb Ahmad, Muhammad Ali Shah, Xun Bo Zhou","doi":"10.1093/plphys/kiae638","DOIUrl":"https://doi.org/10.1093/plphys/kiae638","url":null,"abstract":"Nickel oxide nanoparticles (NiO-NPs) pose potential threats to agricultural production. Bacillus subtilis has emerged as a stress-mitigating microbe that alleviates the phytotoxicity caused by NiO-NPs. However, the mechanisms underlying its effectiveness, particularly in root-nodule symbiosis and biological N2-fixation (BNF), remain unclear. Here, we tested the combined exposure of NiO-NPs (50 mg kg-1) and B. subtilis on soybean (Glycine max L.) growth and BNF. Combined exposure increased root length, shoot length, root biomass, and shoot biomass by 19-26%, while Ni (200 mg kg-1) reduced them by 38--53% compared to the control. NiO-NPs at 100 and 200 mg kg-1 significantly (P < 0.05) reduced nodule formation by 16% and 58% and Nitrogen assimilation enzyme activities levels (UE, NR, HS, and GOGAT) by 13-57%. However, co-exposure with B. subtilis improved nodule formation by 22-44%. Co-exposure of NiO-NPs (200 mg kg-1) with B. subtilis increased POD, CAT, and GSH-Px activity levels by 20%, 16%, and 14% while reducing MDA (14%) and H₂O₂ (12%) levels compared to NiO-NPs alone. Additionally, co-exposure of NiO-NPs (100 and 200 mg kg-1) with B. subtilis enhanced the relative abundance of Stenotrophomonas, Gemmatimonas, and B. subtilis, is associated with N2-cycling and N2-fixation potential. This study confirms that B. subtilis effectively mitigates NiO-NP toxicity in soybean, offering a sustainable method to enhance BNF and crop growth and contribute to addressing global food insecurity.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Refinement of the Brassica napus NLRome using RenSeq. 利用 RenSeq 完善甘蓝菜 NLRome。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-28 DOI: 10.1093/plphys/kiae631

Jiaxu Wu, Soham Mukhopadhyay, Edel Pérez-López

引用次数: 0

SORTING NEXIN1 facilitates SALT OVERLY SENSITIVE1 protein accumulation to enhance salt tolerance in Arabidopsis. 分拣 NEXIN1 可促进拟南芥中盐过度敏感 1 蛋白的积累，从而增强其耐盐性。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-11-28 DOI: 10.1093/plphys/kiae633

Ru-Feng Song, Cai-Yi Liao, Lin-Feng Wang, Kai-Kai Lu, Chi Zhang, Run-Xin Wu, Ji-Xiao Wu, Yu-Qing Ma, Lei Kuang, Ning Guo, Hong-Mei Yuan, Wen-Cheng Liu

{"title":"SORTING NEXIN1 facilitates SALT OVERLY SENSITIVE1 protein accumulation to enhance salt tolerance in Arabidopsis.","authors":"Ru-Feng Song, Cai-Yi Liao, Lin-Feng Wang, Kai-Kai Lu, Chi Zhang, Run-Xin Wu, Ji-Xiao Wu, Yu-Qing Ma, Lei Kuang, Ning Guo, Hong-Mei Yuan, Wen-Cheng Liu","doi":"10.1093/plphys/kiae633","DOIUrl":"https://doi.org/10.1093/plphys/kiae633","url":null,"abstract":"The plasma membrane (PM)-localized Na+/H+ antiporter Salt Overly Sensitive1 (SOS1) is essential for plant salt tolerance through facilitating Na+ efflux; however, how SOS1 localization and protein accumulation is regulated in plants remains elusive. Here, we report that Sorting Nexin 1 (SNX1) is required for plant salt stress tolerance through affecting endosomal trafficking of SOS1 in Arabidopsis (Arabidopsis thaliana). Disruption of SNX1 caused salt hypersensitivity with increased Na+ accumulation and decreased Na+ efflux in Arabidopsis when challenged with high salinity stress. SNX1 co-localized and interacted with SOS1 in endosomes, promoting its PM localization and protein stability in plants under saline conditions. SOS1 overexpression promoted salt tolerance in the wild-type, whereas such effect was greatly compromised in the snx1-2 mutant. Pharmaceutical results showed that SOS1 recycling from the cytosol to the PM was largely blocked while its vacuolar degradation was accelerated in the snx1-2 mutant. Furthermore, salt-induced SOS1 phosphorylation enhanced its interaction and co-localization with SNX1, which is required for SOS1 PM localization in plants. Our study elucidates that SNX1 facilitates SOS1 PM localization and protein accumulation through endosomal trafficking, thereby enhancing salt tolerance in plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclic nucleotide-gated ion channel 20 regulates melatonin-induced calcium signaling and cold tolerance in watermelon 环核苷酸门控离子通道 20 调节褪黑激素诱导的钙信号传导和西瓜的耐寒性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-26 DOI: 10.1093/plphys/kiae630

Jingjing Chang, Yanliang Guo, Jiayue Li, Lingling Liu, Jiahe Liu, Li Yuan, Chunhua Wei, Jianxiang Ma, Yong Zhang, Golam Jalal Ahammed, Feishi Luan, Yunqi Liu, Xian Zhang, Hao Li

{"title":"Cyclic nucleotide-gated ion channel 20 regulates melatonin-induced calcium signaling and cold tolerance in watermelon","authors":"Jingjing Chang, Yanliang Guo, Jiayue Li, Lingling Liu, Jiahe Liu, Li Yuan, Chunhua Wei, Jianxiang Ma, Yong Zhang, Golam Jalal Ahammed, Feishi Luan, Yunqi Liu, Xian Zhang, Hao Li","doi":"10.1093/plphys/kiae630","DOIUrl":"https://doi.org/10.1093/plphys/kiae630","url":null,"abstract":"Melatonin plays a crucial role in regulating plant cold tolerance, but the mechanisms underlying signal transduction remain elusive. In this study, we discovered that overexpression of the melatonin biosynthetic gene caffeic acid O-methyltransferase1 (COMT1) enhanced watermelon (Citrullus lanatus) cold tolerance, accompanied by the accumulation of cytosolic free calcium ([Ca2+]cyt), a stimulation of Ca2+ influx, and upregulation of four Ca2+-permeable channel genes (CNGC2/10/17/20). Conversely, knockout of COMT1 exhibited contrasting effects compared to its overexpression. Knocking out the four CNGC genes revealed that only CNGC20 mediates melatonin-induced Ca2+ influx in response to cold stimuli. CNGC20 deletion impeded watermelon callus redifferentiation, prompting us to employ a virus-induced gene silencing strategy to suppress its expression. Silencing CNGC20 compromised COMT1 overexpression-induced [Ca2+]cyt accumulation, Ca2+ influx, and watermelon cold tolerance. Yeast two-hybrid, bimolecular fluorescence complementation, firefly luciferase complementation imaging, and pull-down assays revealed an interaction between CNGC20 and calmodulin7 (CaM7). Overexpressing CaM7 inhibited melatonin-induced [Ca2+]cyt accumulation, Ca2+ influx, and watermelon cold tolerance. Conversely, silencing CaM7 increased [Ca2+]cyt accumulation, Ca2+ influx, and cold tolerance, whereas COMT1 overexpression failed to further enhance these responses in CaM7-silenced plants, indicating the negative regulation role of CaM7 in melatonin-mediated cold responses. Overall, these findings provide insights into the molecular mechanisms underlying melatonin-enhanced plant cold tolerance via Ca2+ signaling, holding potential for breeding/engineering cold-tolerant cucurbit varieties.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"77 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142718417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WD40 protein OsTTG1 promotes anthocyanin accumulation and CBF transcription factor-dependent pathways for rice cold tolerance WD40 蛋白 OsTTG1 促进花青素积累和水稻耐寒的 CBF 转录因子依赖途径

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-11-26 DOI: 10.1093/plphys/kiae604

Chenli Zhu, Xinghai Yang, Weiwei Chen, Xiuzhong Xia, Zhongqiong Zhang, Dongjin Qing, Baoxuan Nong, Jingcheng Li, Shuhui Liang, Shuangshuang Luo, Weiyong Zhou, Yong Yan, Gaoxing Dai, Danting Li, Guofu Deng

{"title":"WD40 protein OsTTG1 promotes anthocyanin accumulation and CBF transcription factor-dependent pathways for rice cold tolerance","authors":"Chenli Zhu, Xinghai Yang, Weiwei Chen, Xiuzhong Xia, Zhongqiong Zhang, Dongjin Qing, Baoxuan Nong, Jingcheng Li, Shuhui Liang, Shuangshuang Luo, Weiyong Zhou, Yong Yan, Gaoxing Dai, Danting Li, Guofu Deng","doi":"10.1093/plphys/kiae604","DOIUrl":"https://doi.org/10.1093/plphys/kiae604","url":null,"abstract":"Temperature is a critical abiotic factor affecting rice (Oryza sativa L.) yields, and cold stress at the seedling stage can inhibit plant growth or even be fatal. Antioxidants such as anthocyanins accumulate in a variety of plants during cold stress, but the underlying mechanisms are not well understood. Here, we report that rice TRANSPARENT TESTA GLABRA 1 (OsTTG1), a major regulator of anthocyanin biosynthesis in rice, responds to short- and long-term cold stress at both the transcriptional and protein levels. Metabolomic and transcriptomic data indicate that OsTTG1 activates the expression of anthocyanidin synthase (OsANS) genes under cold stress. Our data also suggest that OsTTG1 forms a MYB–bHLH–WD (MBW) complex with Basic helix-loop-helix 148 (OsbHLH148) and Myb-related S3 (OsMYBS3), and this complex activates the expression of Dehydration-responsive element-binding protein 1 (OsDREB1) and OsANS genes. Together, our findings reveal the mechanisms by which OsTTG1 coordinates both anthocyanin biosynthesis and the expression of cold-responsive genes in colored rice, providing genetic resources for future cold resistance breeding in rice.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"68 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142718418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0