Plant Physiology最新文献_第8页

Osmotic stress in roots drives lipoxygenase-dependent plastid remodeling through singlet oxygen production. 根中的渗透胁迫通过产生单线态氧驱动脂氧合酶依赖性质体重塑

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-23 DOI: 10.1093/plphys/kiae589

Dekel Cohen-Hoch, Tomer Chen, Lior Sharabi, Nili Dezorella, Maxim Itkin, Gil Feiguelman, Sergey Malitsky, Robert Fluhr

{"title":"Osmotic stress in roots drives lipoxygenase-dependent plastid remodeling through singlet oxygen production.","authors":"Dekel Cohen-Hoch, Tomer Chen, Lior Sharabi, Nili Dezorella, Maxim Itkin, Gil Feiguelman, Sergey Malitsky, Robert Fluhr","doi":"10.1093/plphys/kiae589","DOIUrl":"10.1093/plphys/kiae589","url":null,"abstract":"Osmotic stress, caused by the lack of water or by high salinity, is a common problem in plant roots. Osmotic stress can be reproducibly simulated with the application of solutions of the high-molecular-weight and impermeable polyethylene glycol. The accumulation of different reactive oxygen species, such as singlet oxygen, superoxide, and hydrogen peroxide, accompany this stress. Among them, singlet oxygen, produced as a byproduct of lipoxygenase activity, has been associated with limiting root growth. To better understand the source and effect of singlet oxygen, we followed its production at the cellular level in Arabidopsis (Arabidopsis thaliana). Osmotic stress initiated profound changes in plastid and vacuole structure. Confocal and electron microscopy showed that the plastids were a source of singlet oxygen accompanied by the appearance of multiple, small extraplastidic bodies that were also an intense source of singlet oxygen. A marker protein, CRUMPLED LEAF, indicated that these small bodies originated from the plastid outer membrane. Remarkably, LINOLEATE 9S-LIPOXYGENASE 5 (LOX5) was shown to change its distribution from uniformly cytoplasmic to a more clumped distribution together with plastids and the small bodies. In addition, oxylipin products of Type 9 lipoxygenase increased, while products of Type 13 lipoxygenases decreased. Inhibition of lipoxygenase by the salicylhydroxamic acid inhibitor or in downregulated lipoxygenase lines prevented cells from initiating the cellular responses, leading to cell death. In contrast, singlet oxygen scavenging halted terminal cell death. These findings underscore the reversible nature of osmotic stress-induced changes, emphasizing the pivotal roles of lipoxygenases and singlet oxygen in root stress physiology.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-exposure impact of nickel oxide nanomaterials and Bacillus subtilis on soybean growth and nitrogen assimilation dynamics. 氧化镍纳米材料与枯草芽孢杆菌共暴露对大豆生长和氮同化动力学的影响

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-23 DOI: 10.1093/plphys/kiae638

Kashif Khan, Zhen Wei Li, Rayyan Khan, Shahid Ali, Haseeb Ahmad, Muhammad Ali Shah, Xun Bo Zhou

{"title":"Co-exposure impact of nickel oxide nanomaterials and Bacillus subtilis on soybean growth and nitrogen assimilation dynamics.","authors":"Kashif Khan, Zhen Wei Li, Rayyan Khan, Shahid Ali, Haseeb Ahmad, Muhammad Ali Shah, Xun Bo Zhou","doi":"10.1093/plphys/kiae638","DOIUrl":"10.1093/plphys/kiae638","url":null,"abstract":"Nickel oxide nanoparticles (NiO-NPs) pose potential threats to agricultural production. Bacillus subtilis has emerged as a stress-mitigating microbe that alleviates the phytotoxicity caused by NiO-NPs. However, the mechanisms underlying its effectiveness, particularly in root-nodule symbiosis and biological N2-fixation (BNF), remain unclear. Here, we tested the combined exposure of NiO-NPs (50 mg kg-1) and B. subtilis on soybean (Glycine max L.) growth and BNF. Combined exposure increased root length, shoot length, root biomass, and shoot biomass by 19% to 26%, while Ni (200 mg kg-1) reduced them by 38% to 53% compared to the control. NiO-NPs at 100 and 200 mg kg-1 significantly (P < 0.05) reduced nodule formation by 16% and 58% and Nitrogen assimilation enzyme activities levels (urease, nitrate reductase, glutamine synthetase, and glutamate synthetase) by 13% to 57%. However, co-exposure with B. subtilis improved nodule formation by 22% to 44%. Co-exposure of NiO-NPs (200 mg kg-1) with B. subtilis increased peroxidase, catalase, and glutathione peroxidase activity levels by 20%, 16%, and 14% while reducing malondialdehyde (14%) and hydrogen peroxide (12%) levels compared to NiO-NPs alone. Additionally, co-exposure of NiO-NPs (100 and 200 mg kg-1) with B. subtilis enhanced the relative abundance of Stenotrophomonas, Gemmatimonas, and B. subtilis, is associated with N2-cycling and N2-fixation potential. This study confirms that B. subtilis effectively mitigates NiO-NP toxicity in soybean, offering a sustainable method to enhance BNF and crop growth and contribute to addressing global food insecurity.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

m6A and m5C modifications as the gears: CmoCK1 mRNA travels to promote chilling tolerance. m6A 和 m5C 修饰作为齿轮：CmoCK1 mRNA 移动促进耐寒性。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-23 DOI: 10.1093/plphys/kiae572

Yee-Shan Ku

引用次数: 0

A simplified Agrobacterium tumefaciens-mediated transformation protocol accelerates plant molecular breeding. 农杆菌介导的简化转化协议加速了植物分子育种。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-23 DOI: 10.1093/plphys/kiae585

Qianqian Li, Xingli Zhang, Haiyun Li, Huihui Zhang, Hongyan Hao, Wenjie Yu, Jinbian Sun, Yinglun Fan, Shanhua Lyu

引用次数: 0

Tools to understand hypoxia responses in plant tissues. 了解植物组织缺氧反应的工具。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-23 DOI: 10.1093/plphys/kiae624

Gabriele Panicucci, Pedro Barreto, Max Herzog, Sophie Lichtenauer, Markus Schwarzländer, Ole Pedersen, Daan A Weits

{"title":"Tools to understand hypoxia responses in plant tissues.","authors":"Gabriele Panicucci, Pedro Barreto, Max Herzog, Sophie Lichtenauer, Markus Schwarzländer, Ole Pedersen, Daan A Weits","doi":"10.1093/plphys/kiae624","DOIUrl":"10.1093/plphys/kiae624","url":null,"abstract":"Our understanding of how low oxygen (O2) conditions arise in plant tissues and how they shape specific responses has seen major advancement in recent years. Important drivers have been (1) the discovery of the molecular machinery that underpins plant O2 sensing; and (2) a growing set of dedicated tools to define experimental conditions and assess plant responses with increasing accuracy and resolution. While some of those tools, such as the Clark-type O2 electrode, were established decades ago, recent customization has set entirely new standards and enabled novel research avenues in plant hypoxia research. Other tools, such as optical hypoxia reporters and O2 biosensor systems, have been introduced more recently. Yet, their adoption into plant hypoxia research has started to generate novel insight into hypoxia physiology at the tissue and cellular levels. The aim of this update is to provide an overview of the currently available and emerging tools for O2 hypoxia measurements in plants, with an emphasis on high-resolution analyses in living plant tissues and cells. Furthermore, it offers directions for future development and deployment of tools to aid progress with the most pressing questions in plant hypoxia research.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phos-fate of the liverwort: Unlocking the secrets to Pi homeostasis in Marchantia polymorpha. 肝草的磷脂命运：揭开马钱子藻π平衡的秘密。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-23 DOI: 10.1093/plphys/kiae522

Jiawen Chen, Nicola Trozzi

引用次数: 0

Primed to persevere: Hypoxia regulation from epigenome to protein accumulation in plants. 有备无患：植物从表观基因组到蛋白质积累的缺氧调控。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-23 DOI: 10.1093/plphys/kiae584

Daniel J Gibbs, Frederica L Theodoulou, Julia Bailey-Serres

{"title":"Primed to persevere: Hypoxia regulation from epigenome to protein accumulation in plants.","authors":"Daniel J Gibbs, Frederica L Theodoulou, Julia Bailey-Serres","doi":"10.1093/plphys/kiae584","DOIUrl":"10.1093/plphys/kiae584","url":null,"abstract":"Plant cells regularly encounter hypoxia (low-oxygen conditions) as part of normal growth and development, or in response to environmental stresses such as flooding. In recent years, our understanding of the multi-layered control of hypoxia-responsive gene expression has greatly increased. In this Update, we take a broad look at the epigenetic, transcriptional, translational, and post-translational mechanisms that regulate responses to low-oxygen levels. We highlight how a network of post-translational modifications (including phosphorylation), secondary messengers, transcriptional cascades, and retrograde signals from the mitochondria and endoplasmic reticulum (ER) feed into the control of transcription factor activity and hypoxia-responsive gene transcription. We discuss epigenetic mechanisms regulating the response to reduced oxygen availability, through focussing on active and repressive chromatin modifications and DNA methylation. We also describe current knowledge of the co- and post-transcriptional mechanisms that tightly regulate mRNA translation to coordinate effective gene expression under hypoxia. Finally, we present a series of outstanding questions in the field and consider how new insights into the molecular workings of the hypoxia-triggered regulatory hierarchy could pave the way for developing flood-resilient crops.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutation of GLR2 confers enhanced glufosinate resistance and salt tolerance in rice. GLR2 基因突变增强了水稻的草铵膦抗性和耐盐性。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-23 DOI: 10.1093/plphys/kiae588

Weimin Cheng, Yan Ren, Jiayi Wang, Chunpeng Chen, Cheng Fang, Lingling Peng, Dongyang Zhang, Liangzhi Tao, Yue Zhan, Kun Wu, Yuejin Wu, Binmei Liu, Xiangdong Fu, Yafeng Ye

引用次数: 0

Lipid droplets: New roles as mediators of biotic and abiotic stress. 脂滴：作为生物和非生物压力介质的新角色。

IF 6.5 1区生物学

Plant Physiology Pub Date : 2024-12-23 DOI: 10.1093/plphys/kiae340

Hannah M McMillan

引用次数: 0

Generating fragrant oilseed rape using CRISPR/Cas9-mediated gene editing 利用CRISPR/ cas9介导的基因编辑技术生成芳香油菜

IF 7.4 1区生物学

Plant Physiology Pub Date : 2024-12-23 DOI: 10.1093/plphys/kiae660

Jian Wu, Jingyi Xu, Ancheng He, Sichao Ren, Yi Ye, Wenjing Lei, Yu Liu, Xia Hua, Chunjie Wei, Li Lin, Hui Zhang, Youping Wang

引用次数: 0