Plant Physiology最新文献_第3页

Ethylene regulates RGL2a homeostasis and gibberellin signaling responses by coordinating F-box protein EBF1 and kinase MPK7 in apple 乙烯通过调控苹果F-box蛋白EBF1和激酶MPK7调控RGL2a稳态和赤霉素信号响应

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-03 DOI: 10.1093/plphys/kiaf484

Miao-Yi Li, Lei Zhao, Di Ai, Yanru Hu, Yuepeng Han, Jian-Ping An

{"title":"Ethylene regulates RGL2a homeostasis and gibberellin signaling responses by coordinating F-box protein EBF1 and kinase MPK7 in apple","authors":"Miao-Yi Li, Lei Zhao, Di Ai, Yanru Hu, Yuepeng Han, Jian-Ping An","doi":"10.1093/plphys/kiaf484","DOIUrl":"https://doi.org/10.1093/plphys/kiaf484","url":null,"abstract":"Although ethylene and gibberellin (GA) coordinately regulate plant growth, development, and stress responses, the specifics of how ethylene regulates GA signaling are not well understood. In this study, we clarified the molecular mechanism by which ethylene regulates GA signaling in anthocyanin biosynthesis in apple (Malus × domestica): ethylene promotes the ubiquitination and phosphorylation of a DELLA protein, a central regulator of GA signaling. Our findings revealed that ethylene and GA antagonistically regulate anthocyanin biosynthesis, with ethylene impairing the ubiquitination of the DELLA protein REPRESSOR-of-ga1-3-LIKE 2a (MdRGL2a). The F-box protein ETHYLENE-INSENSITIVE 3-BINDING F-BOX PROTEIN 1 (MdEBF1), a key regulator of ethylene signaling, destabilizes MdRGL2a via the 26S proteasome pathway. Conversely, the ethylene-activated protein kinase MITOGEN-ACTIVATED PROTEIN KINASE 7 (MdMPK7) enhances MdRGL2a stability by protecting it from MdEBF1-mediated degradation. Overall, our results demonstrate that ethylene regulates MdRGL2a homeostasis and GA signaling responses by coordinating MdEBF1 and MdMPK7. These insights advance our understanding of the post-translational modifications of DELLA proteins, GA signal transduction, and phytohormone crosstalk in anthocyanin biosynthesis.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"99 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145215634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LpBPM5.6 regulates turfgrass architecture by influencing LpARG1 stability in perennial ryegrass LpBPM5.6通过影响多年生黑麦草LpARG1稳定性调控草坪草结构

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-03 DOI: 10.1093/plphys/kiaf480

Cuicui Miao, Zhijian Cao, Zhen Zeng, Liaoliao Ye, Ruixin Chen, Xiuhua Tang, Wenting Qiao, Jing Wang, Zhiquan Qiang, Tao Qin

{"title":"LpBPM5.6 regulates turfgrass architecture by influencing LpARG1 stability in perennial ryegrass","authors":"Cuicui Miao, Zhijian Cao, Zhen Zeng, Liaoliao Ye, Ruixin Chen, Xiuhua Tang, Wenting Qiao, Jing Wang, Zhiquan Qiang, Tao Qin","doi":"10.1093/plphys/kiaf480","DOIUrl":"https://doi.org/10.1093/plphys/kiaf480","url":null,"abstract":"The improvement of plant architecture has historically driven the “Green Revolution”, leading to substantial increases in cereal yields. However, the architectural requirements for turfgrass differ from those of cereals. Despite its importance, our understanding of the mechanisms that regulate plant architecture in turfgrass remains limited. In this study, we identified that Lolium perenne BTB/POZ-MATH5.6s (LpBPM5.6) plays a crucial role in shaping the ideal turfgrass architecture by influencing the stability of ALTERED RESPONSE TO GRAVITY1 (LpARG1) in perennial ryegrass. The phenotypes of LpBPM5.6-overexpressing and RNAi transgenic seedlings revealed that knocking down LpBPM5.6 results in an ideal turfgrass architecture characterized by reduced plant height, increased tillering, and less fibrous roots. Through yeast two-hybrid library screening, as well as subsequent pull-down, luciferase complementation imaging, and co-immunoprecipitation techniques, we demonstrated that LpBPM5.6 interacts with LpARG1. Protein degradation assays indicated that LpBPM5.6 regulates the degradation of LpARG1. Additionally, microscopic observations showed that LpBPM5.6 and LpARG1 affect the trafficking of PIN1, thereby influencing auxin transport and turfgrass architecture. This study elucidates how LpBPM5.6 regulates turfgrass architecture by modulating the stability of LpARG1, providing insights into the genetic mechanisms underlying the ideal plant architecture for turfgrass.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145215635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CYP81D11, an apocarotenoid-responsive cytochrome P450, enhances photosynthesis and stress tolerance in plants CYP81D11是一种对类胡萝卜素敏感的细胞色素P450，它能增强植物的光合作用和抗逆性

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-03 DOI: 10.1093/plphys/kiaf476

Madhu Tiwari, Brigitte Ksas, Bertrand Légeret, Stefania Viola, Stefano Caffarri, Michel Havaux

{"title":"CYP81D11, an apocarotenoid-responsive cytochrome P450, enhances photosynthesis and stress tolerance in plants","authors":"Madhu Tiwari, Brigitte Ksas, Bertrand Légeret, Stefania Viola, Stefano Caffarri, Michel Havaux","doi":"10.1093/plphys/kiaf476","DOIUrl":"https://doi.org/10.1093/plphys/kiaf476","url":null,"abstract":"β-cyclocitric acid and its precursor β-cyclocitral are signaling apocarotenoids that trigger defense and detoxification mechanisms enhancing plant tolerance to abiotic stresses. From a transcriptomic analysis of Arabidopsis (Arabidopsis thaliana) plants exposed to each apocarotenoid over several exposure times, we identified a gene (CYP81D11) encoding a cytochrome P450 that is strongly induced under all conditions and is under the control of the TGAII-SCL14 transcription regulator. Overexpressing the CYP81D11 gene in Arabidopsis led to a high tolerance to high light and water stresses while a CYP81D11-deficient mutant was sensitive to both stress conditions. CYP81D11 expression levels were inversely correlated with the accumulation of reactive carbonylated lipid-oxidation products in leaves. The transcriptomic profile of the CYP81D11 overexpressor revealed a selective upregulation of genes related to photosynthesis and response to high light stress. High expression levels of CYP81D11 enhanced photosynthetic electron transport, CO2 fixation and biomass production. These effects occurred in high light, not in low light, and were associated with a noticeable decrease in singlet oxygen photoproduction. These findings indicate that CYP81D11 is a key component in the regulation of plant stress tolerance, enhancing the photosynthetic capacity of leaves and preventing accumulation of reactive carbonyls. This gene could be a target for improving photosynthesis in high-light environments.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"8 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145215686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The peptide EPFL8 represses embryonic stomatal precursor formation independently of TOO MANY MOUTHS in Arabidopsis 肽EPFL8在拟南芥中独立于TOO MANY mouth抑制胚胎气孔前体的形成

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-02 DOI: 10.1093/plphys/kiaf487

Qin He, Aditya Birla, Guillaume Lamoureux, Huiliang Zhang, Xingyun Qi

{"title":"The peptide EPFL8 represses embryonic stomatal precursor formation independently of TOO MANY MOUTHS in Arabidopsis","authors":"Qin He, Aditya Birla, Guillaume Lamoureux, Huiliang Zhang, Xingyun Qi","doi":"10.1093/plphys/kiaf487","DOIUrl":"https://doi.org/10.1093/plphys/kiaf487","url":null,"abstract":"Plants regulate gas exchange through stomata, which are specialized valves on the aerial epidermis. Stomatal development involves tightly controlled transitions orchestrated by basic helix-loop-helix transcription factors, including SPEECHLESS (SPCH). These factors coordinate cell division and differentiation during stomatal development. Recent studies have underscored the importance of cell–cell communication in this process, mediated by secreted peptide ligands of the EPIDERMAL PATTERNING FACTOR (EPF) family and a Leucine-Rich-Repeat Receptor complex that includes ERECTA family receptor kinases and the receptor TOO MANY MOUTHS (TMM). Although mature stomata do not form during embryogenesis, stomatal cell fate is determined at these early stages. In this study, we discovered that EPFL8 is highly expressed during early embryogenesis and inhibits stomatal development by negatively regulating SPCH. Furthermore, TMM, a SPCH target, interferes with EPFL8-mediated signaling. In the absence of TMM, EPFL8 expression extends into later embryogenesis, which aligns with the reduced number of stomatal precursors observed in tmm mutants. These findings reveal that EPFL8 functions as an embryonic peptide ligand that suppresses stomatal formation via SPCH regulation, highlighting the diversification of EPF family members in fine-tuning plant epidermal patterning.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145235328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The zinc finger transcription factor SlZFP2 regulates locular tissue morphogenesis 锌指转录因子SlZFP2调控细胞室组织形态发生

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-01 DOI: 10.1093/plphys/kiaf421

Gabriel Hoang, Jorly Joana, Dario Constantinescu, Pascal G P Martin, Stéphanie Gadin, Jean-Philippe Mauxion, Cécile Brès, Virginie Garcia, Nathalie Gonzalez, Christophe Rothan, Nadia Bertin, Lucie Fernandez-Lochu, Martine Lemaire-Chamley

{"title":"The zinc finger transcription factor SlZFP2 regulates locular tissue morphogenesis","authors":"Gabriel Hoang, Jorly Joana, Dario Constantinescu, Pascal G P Martin, Stéphanie Gadin, Jean-Philippe Mauxion, Cécile Brès, Virginie Garcia, Nathalie Gonzalez, Christophe Rothan, Nadia Bertin, Lucie Fernandez-Lochu, Martine Lemaire-Chamley","doi":"10.1093/plphys/kiaf421","DOIUrl":"https://doi.org/10.1093/plphys/kiaf421","url":null,"abstract":"In tomato (Solanum lycopersicum L.) fruit, locular tissue (LT) is a unique jelly-like structure that differentiates from the central axis of the fruit following ovule fertilization. LT is essential for seed development and dispersal, preventing early germination, and initiating fruit ripening. In this work, we studied a “gel-less” mutant and identified the underlying mutation in the coding sequence of the C2H2 zinc finger transcription factor (TF) SlZFP2. Histological, cytological and molecular characterization of zfp2-CRISPR/Cas9 knockout lines revealed an early and strong impact of zfp2 disruption on cell division and endoreduplication in LT. Further, model-based analysis of cellular data revealed that the cell division was the main altered process explaining the zfp2 mutant phenotype. RNA-seq analysis of young LT further highlighted global transcriptional changes of cell cycle-related genes between WT and zfp2 mutants, providing insight into the molecular players involved in locular tissue differentiation. This multi-faceted approach uncovers SlZFP2 as a regulator of LT morphogenesis and reveals its main action in regulating cell division. Since few regulators of LT developmental processes have been described so far, this work on SlZFP2 establishes groundwork for future studies aimed at deciphering the complex regulatory networks governing fruit tissue development.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"115 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The gibberellin-activated transcription factor MdRAV1 regulates ethylene biosynthesis to suppress apple fruit ripening 赤霉素激活转录因子MdRAV1调控乙烯生物合成抑制苹果果实成熟

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-10-01 DOI: 10.1093/plphys/kiaf436

Shijiao Lin, Mingyang Xu, Yuling Liang, Mingqian Wang, Yunyan Peng, Yanan Wang, Weiting Liu, Aide Wang, Yinglin Ji

{"title":"The gibberellin-activated transcription factor MdRAV1 regulates ethylene biosynthesis to suppress apple fruit ripening","authors":"Shijiao Lin, Mingyang Xu, Yuling Liang, Mingqian Wang, Yunyan Peng, Yanan Wang, Weiting Liu, Aide Wang, Yinglin Ji","doi":"10.1093/plphys/kiaf436","DOIUrl":"https://doi.org/10.1093/plphys/kiaf436","url":null,"abstract":"The phytohormone ethylene is vital for the ripening of climacteric fruit, such as apples (Malus domestica). The gibberellin (GA) class of phytohormones affects ethylene biosynthesis during ripening via an unknown molecular mechanism. Here, we investigated the effects of GAs on apple fruit ripening. We observed that endogenous GA accumulation was opposite to that of ethylene production during apple fruit development. Moreover, exogenous GA treatment suppressed ethylene production and fruit ripening. However, treatment with a GA biosynthesis inhibitor promoted ethylene production and accelerated fruit ripening in apple, suggesting that GA suppresses ripening. GA treatment enhanced the expression of the transcription factor Related to ABSCISIC ACID INSENSITIVE3/VIVIPAROUS1 (MdRAV1) during apple ripening. The DELLA protein GA INSENSITIVE (MdGAI) interacted with MdRAV1 and suppressed its DNA-binding and regulatory activities. GA triggered MdGAI removal, thereby releasing MdRAV1. GA-activated MdRAV1 bound to the promoter of MdACS1, encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, and directly suppressed its transcription. These results suggest that GA-activated MdRAV1 suppresses MdACS1 transcription, reducing ethylene production and suppressing fruit ripening.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"39 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon sink-source dynamics influence bud awakening under warming and defoliation. 增温和落叶条件下碳汇源动态影响芽苏醒。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf451

Laura Fernández-de-Uña

引用次数: 0

HyperTRIBE mapping of the RNA m6A demethylase ALKBH9 binding sites in bamboo reveals its role in plant defense 竹中RNA m6A去甲基化酶ALKBH9结合位点的超部落定位揭示了其在植物防御中的作用

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf457

Huihui Wang, Huiyuan Wang, Yue Jia, Tuhe Li, Siyu Yang, Yandong Jin, Zaofa Zhong, Wenting Bai, Huakun Zheng, Liangzhen Zhao, Chentao Lin, Anireddy S N Reddy, Hangxiao Zhang, Lianfeng Gu

{"title":"HyperTRIBE mapping of the RNA m6A demethylase ALKBH9 binding sites in bamboo reveals its role in plant defense","authors":"Huihui Wang, Huiyuan Wang, Yue Jia, Tuhe Li, Siyu Yang, Yandong Jin, Zaofa Zhong, Wenting Bai, Huakun Zheng, Liangzhen Zhao, Chentao Lin, Anireddy S N Reddy, Hangxiao Zhang, Lianfeng Gu","doi":"10.1093/plphys/kiaf457","DOIUrl":"https://doi.org/10.1093/plphys/kiaf457","url":null,"abstract":"RNA demethylation plays an important role in diverse biological processes. Intriguingly, RNA demethylation has not been reported in bamboo, which is known for its rapid growth. PheALKBH9, an m6A demethylase in bamboo, was stably transformed into rice and increased its susceptibility to rice blast disease. Heterologous expression of PheALKBH9 reduced the overall m6A modification levels in rice. Using HyperTRIBE (Targets of RNA-binding proteins Identified By Editing), we identified evolutionarily conserved PheALKBH9 target RNAs in both rice and Moso bamboo. Overexpression of PheALKBH9 led to higher protein expression and shorter poly(A) tails. Notably, PheALKBH9 directly bound to CCR4-associated factor1 (CAF1G) and Poly(A)-binding genes (PABPC1 and PABPC2), potentially modulating poly(A) tail lengths. In addition, PheALKBH9 also bound to and removed m6A modifications from Perox4, JAZ7 and METS2, key players in plant immunity, suggesting that PheALKBH9 plays a role in plant disease resistance. In summary, our study unveils a previously unknown role of PheALKBH9-mediated m6A demethylation in response to blast disease and provides insights into its mechanisms in monocotyledonous plants.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"39 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ALMT12 interacts with and inhibits SLAC1 to modulate stomatal movements and enhance plant biomass ALMT12与SLAC1相互作用，抑制SLAC1调节气孔运动，提高植物生物量

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf460

Ping Lin, Hui Zhou, Qing Zhao, Liumei Li, Jiamei Liu, Zhuoran Hu, Yunxin Luo, Cuizhu Feng, Yu Long

引用次数: 0

The serotonin biosynthesis enzyme T5H5 and transcription factor bZIP23 modulate light-mediated anthocyanin accumulation in apple. 5 -羟色胺生物合成酶T5H5和转录因子bZIP23调节光介导的苹果花青素积累。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2025-09-30 DOI: 10.1093/plphys/kiaf477

Lingling Lv,Lei Bi,Huiru Zhang,Wenyuan Huang,Chunyu Jin,YuXing Li,Wei Liang,Jianwen Feng,Fengwang Ma,Tingting Duan,Cuiying Li

{"title":"The serotonin biosynthesis enzyme T5H5 and transcription factor bZIP23 modulate light-mediated anthocyanin accumulation in apple.","authors":"Lingling Lv,Lei Bi,Huiru Zhang,Wenyuan Huang,Chunyu Jin,YuXing Li,Wei Liang,Jianwen Feng,Fengwang Ma,Tingting Duan,Cuiying Li","doi":"10.1093/plphys/kiaf477","DOIUrl":"https://doi.org/10.1093/plphys/kiaf477","url":null,"abstract":"Serotonin (5-hydroxytryptamine, 5-HT), an indole derivative, has a variety of physiological and developmental functions in plants. Anthocyanins play a crucial role in fruit coloring. Although light controls melatonin biosynthesis in plants, how serotonin, a melatonin precursor, contributes to anthocyanin accumulation at the molecular level remains unknown. In this study, we investigated the potential function of the 5-HT synthetase MdT5H5 in inhibiting anthocyanin accumulation in response to light. In apple fruits, light increased MdT5H5 expression and 5-HT accumulation. Anthocyanin accumulation was reduced in light-induced calli overexpressing MdT5H5, which was also supported by the observation that anthocyanins accumulate in apple fruits when MdT5H5 is transiently silenced. In addition, we identified MdbZIP23 as a repressor of MdT5H5, which promotes anthocyanin accumulation in apple fruits. These results suggest that MdbZIP23 negatively regulates MdT5H5-mediated anthocyanin accumulation, providing insight into the molecular mechanisms of photo-induced anthocyanin accumulation in apple fruits.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"93 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145194730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0