Plant Physiology最新文献

{"title":"Turn up the red: MADS-RIN-DIVARICATA1 module positively regulates carotenoid biosynthesis in nonclimacteric pepper fruits.","authors":"Lara Pereira, Chong Teng","doi":"10.1093/plphys/kiaf104","DOIUrl":"10.1093/plphys/kiaf104","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membranous translation platforms in the chloroplast of Chlamydomonas reinhardtii.","authors":"Yi Sun, Shiva Bakhtiari, Melissa Valente-Paterno, Heng Jiang, William Zerges","doi":"10.1093/plphys/kiaf111","DOIUrl":"10.1093/plphys/kiaf111","url":null,"abstract":"<p><p>A small genome in chloroplasts encodes many of the polypeptide subunits of the photosynthetic electron transport complexes embedded in the membranes of thylakoid vesicles in the chloroplast stroma and synthesized by ribosomes of the bacterial-like genetic system of this semiautonomous organelle. While thylakoid membranes (TMs) are sites of translation, evidence in the unicellular alga Chlamydomonas reinhardtii supports translation on noncanonical membranes in a discrete translation zone in the chloroplast. To characterize the membranous platforms for translation and the biogenesis of TMs, we profiled membranes during chloroplast development, using the yellow-in-the-dark1 mutant, and carried out proteomic analyses on 2 membrane types proposed previously to support translation in the chloroplast of C. reinhardtii: \"low-density membrane\" (LDM) and \"chloroplast translation membrane\" (CTM). The results support the roles of LDM and CTM in the preliminary and ongoing stages of translation, respectively. Proteomics, immunoprecipitation, and transmission electron microscopy results support connections of these membranous platforms and a chloroplast envelope domain bound by cytoplasmic ribosomes. Our results contribute to a model of photosynthesis complex biogenesis in a spatiotemporal \"assembly line\" involving LDM and CTM as sequential stages leading to photosynthetic TMs.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time-course dual RNA-seq analyses and gene identification during early stages of plant-Phytophthora infestans interactions.","authors":"Yanling Cai, Zhiqing Wang, Wei Wan, Jinfeng Qi, Xiao-Feng Liu, Yantao Wang, Yaqing Lyu, Tao Li, Suomeng Dong, Sanwen Huang, Shaoqun Zhou","doi":"10.1093/plphys/kiaf112","DOIUrl":"10.1093/plphys/kiaf112","url":null,"abstract":"<p><p>Late blight caused by Phytophthora infestans is a major threat to global potato and tomato production. Sustainable management of late blight requires the development of resistant crop cultivars. This process can be facilitated by high-throughput identification of functional genes involved in late blight pathogenesis. In this study, we generated a high-quality transcriptomic time-course dataset focusing on the initial 24 h of contact between P. infestans and 3 solanaceous plant species, tobacco (Nicotiana benthamiana), tomato (Solanum lycopersicum), and potato (Solanum tuberosum). Our results demonstrate species-specific transcriptional regulation in early stages of the infection. Transient silencing of putative RIBOSE-5-PHOSPHATE ISOMERASE and HMG-CoA REDUCTASE genes in N. benthamiana lowered plant resistance against P. infestans. Furthermore, heterologous expression of a putative tomato Golgi-localized nucleosugar transporter-encoding gene exacerbated P. infestans infection of N. benthamiana. In comparison, bioassays using transgenic tomato lines showed that the quantitative disease resistance genes were required but insufficient for late blight resistance; genetic knock-out of the susceptibility gene enhanced resistance. The same RNA-seq dataset was exploited to examine the transcriptional landscape of P. infestans and revealed host-specific gene expression patterns in the pathogen. This temporal transcriptomic diversity, in combination with genomic distribution features, identified the P. infestans IPI-B family GLYCINE-RICH PROTEINs as putative virulence factors that promoted disease severity or induced plant tissue necrosis when transiently expressed in N. benthamiana. These functional genes underline the effectiveness of functional gene-mining through a time-course dual RNA-seq approach and provide insight into the molecular interactions between solanaceous plants and P. infestans.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Citrus yellow vein clearing virus infection triggers phloem remobilization of iron- and zinc-nicotianamine in citrus.","authors":"","doi":"10.1093/plphys/kiaf091","DOIUrl":"https://doi.org/10.1093/plphys/kiaf091","url":null,"abstract":"","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"197 4","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TTLOC: A Tn5 transposase-based approach to localize T-DNA integration sites.","authors":"Xiao-Yuan Tao, Shou-Li Feng, Xin-Jia Li, Yan-Jun Li, Wei Wang, Matthew Gilliham, Zhong-Hua Chen, Sheng-Chun Xu","doi":"10.1093/plphys/kiaf102","DOIUrl":"10.1093/plphys/kiaf102","url":null,"abstract":"<p><p>Thermal asymmetric interlaced-polymerase chain reaction-based and whole-genome sequencing-based T-DNA localization approaches have been developed for the recovery of T-DNA integration sites (TISs). Nevertheless, a low-cost and high-throughput technique for the detection of TISs, which would facilitate the identification of genetically engineered plants, is in high demand for rapid crop breeding and plant synthetic biology. Here, we present Tn5 transposase-based T-DNA integration site localization (TTLOC), a Tn5-based approach for TIS localization. TTLOC employs specialized adaptor-assembled Tn5 transposases for genomic DNA tagmentation. TTLOC library construction is straightforward, involving only six steps that requires two and a half hours to complete. The resulting pooled library is compatible with next-generation sequencing, which enables high-throughput determination. We demonstrate the ability of TTLOC to recover 95 non-redundant TISs from 65 transgenic Arabidopsis (Arabidopsis thaliana) lines, and 37 non-redundant TISs from the genomes of transgenic rice (Oryza sativa), soybean (Glycine max), tomato (Solanum lycopersicum), potato (Solanum tuberosum), and from the large hexaploid wheat (Triticum aestivum) genome. TTLOC is a cost-effective method, as 1 to 2 Gb of raw data for each multiplexing library are sufficient for efficient TIS calling, independent of the genome size. Our results establish TTLOC as a promising strategy for evaluation of genome engineered plants and for selecting genome safe harbors for trait stacking in crop breeding and plant synthetic biology.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11961865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transcription factors MYB80 and TEK coordinate callose wall degradation and pollen exine formation in Arabidopsis.","authors":"Xiaofeng Xu, Kaiqi Wang, Yahui Yu, Xin Zhao, Yuyi Guo, Yaqi Liu, Xuexue Qian, Naiying Yang, Ping Xu, Zhong-Nan Yang","doi":"10.1093/plphys/kiaf124","DOIUrl":"https://doi.org/10.1093/plphys/kiaf124","url":null,"abstract":"<p><p>Pollen development involves cell wall alteration of the male gametophyte, which is critical for plant fertility and requires MYB80 and TRANSPOSABLE ELEMENT SILENCING VIA AT-HOOK (TEK) transcription factors in Arabidopsis (Arabidopsis thaliana). In this study, we found that the myb80 tek double mutant exhibits a compromised degradation of the tetrad callose wall and down-regulation of five ANTHER-SPECIFIC PROTEIN 6 (A6) genes encoding β-1,3-glucanase. The quintuple mutant of A6 (a6-quint) exhibited delayed callose wall degradation and defective exine structure, and its pollen had a weakened UV resistance. The quadruple mutant of A6 (a6-quad) restored the fertility of rvms-2, a thermo-sensitive genic male sterile (TGMS) line where the transition from tetrad wall to pollen wall is defective. Transgenic expression of A6 and A6.2 driven by the A9 promoter led to the expression of the two genes in the tapetum at earlier anther developmental stages, which caused premature callose wall dissolution and impeded exine formation, indicating the importance of the temporal control of A6s. Furthermore, dual-luciferase and ChIP assay results confirmed the direct regulation of MYB80 and TEK in activating the expression of the above A6s in the tapetum. In conclusion, callose degradation mediated by the MYB80/TEK-A6s pathway is required for the transition from tetrad callose wall to pollen wall.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The carboxylesterase AtCXE12 converts volatile (Z)-3-hexenyl acetate to (Z)-3-hexenol in Arabidopsis leaves.","authors":"Tristan M Cofer, James H Tumlinson","doi":"10.1093/plphys/kiaf119","DOIUrl":"https://doi.org/10.1093/plphys/kiaf119","url":null,"abstract":"<p><p>The green leaf volatiles (GLVs) (Z)-3-hexenal, (Z)-3-hexenol, and (Z)-3-hexenyl acetate play important roles in plant defense, deterring insect herbivores and attracting their natural enemies, while also serving as airborne signaling molecules capable of enhancing defenses in undamaged plant tissues. Almost all plants produce GLVs after wounding, beginning with the formation of (Z)-3-hexenal, which is subsequently converted to (Z)-3-hexenol and (Z)-3-hexenyl acetate. (Z)-3-hexenyl acetate can then be taken up by nearby plant tissues where it is predicted to be hydrolyzed to (Z)-3-hexenol, a process that is likely to be important in regulating the specific activities of these compounds. However, the enzyme(s) involved in this process and its role in plant defense are largely unknown. Here, we show that Arabidopsis (Arabidopsis thaliana) plants rapidly take up (Z)-3-hexenyl acetate and convert it to (Z)-3-hexenol. Inhibitor and fractionation experiments identified the carboxylesterases Carboxylesterase 5 (AtCXE5) and Carboxylesterase 12 (AtCXE12) as likely contributors to the (Z)-3-hexenyl acetate esterase activity in Arabidopsis leaves. Heterologous expression of AtCXE5 and AtCXE12 in Escherichia colirevealed that both recombinant enzymes hydrolyze (Z)-3-hexenyl acetate to (Z)-3-hexenol. Furthermore, assays using T-DNA insertion mutants showed that AtCXE12 significantly contributes to (Z)-3-hexenyl acetate hydrolysis in Arabidopsis. Lastly, we found that leaves from several other plant species possess (Z)-3-hexenyl acetate esterase activity, suggesting a conserved mechanism for GLV metabolism among plants. Overall, our study provides a better understanding of the biosynthesis and conversion dynamics of GLVs, which is necessary for unraveling the potential functions of these compounds.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutations in HEADING DATE 1 affect transcription and cell wall composition in rice.","authors":"Marco Biancucci, Daniele Chirivì, Alessio Baldini, Eugene Badenhorst, Fabio Dobetti, Bahman Khahani, Elide Formentin, Tenai Eguen, Franziska Turck, John P Moore, Elahe Tavakol, Stephan Wenkel, Fiorella Lo Schiavo, Ignacio Ezquer, Vittoria Brambilla, David Horner, Matteo Chiara, Giorgio Perrella, Camilla Betti, Fabio Fornara","doi":"10.1093/plphys/kiaf120","DOIUrl":"https://doi.org/10.1093/plphys/kiaf120","url":null,"abstract":"<p><p>Plants utilize environmental information to modify their developmental trajectories for optimal survival and reproduction. Over a century ago, day length (photoperiod) was identified as a major factor influencing developmental transitions, particularly the shift from vegetative to reproductive growth. In rice (Oryza sativa), exposure to day lengths shorter than a critical threshold accelerates flowering, while longer days inhibit this process. This response is mediated by HEADING DATE 1 (Hd1), a zinc finger transcription factor that is central in the photoperiodic flowering network. Hd1 acts as a repressor of flowering under long days but functions as a promoter of flowering under short days. However, how global transcription of genes downstream of Hd1 changes in response to the photoperiod is still not fully understood. Furthermore, it is unclear whether Hd1 target genes are solely involved in flowering time control or mediate additional functions. In this study, we utilized RNA-Seq to analyze the transcriptome of hd1 mutants under both long and short day conditions. We identified genes involved in the phenylpropanoid pathway that are deregulated under long days in the mutant. Quantitative profiling of cell wall components and abiotic stress assays suggested that Hd1 is involved in processes considered unrelated to flowering control. This indicates that day length perception and responses are intertwined with physiological processes beyond flowering.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHOTOPERIOD 1 enhances stress resistance and energy metabolism to promote spike fertility in barley under high ambient temperatures.","authors":"Tianyu Lan, Agatha Walla, Kumsal Ecem Çolpan Karışan, Gabriele Buchmann, Vera Wewer, Sabine Metzger, Isaia Vardanega, Einar Baldvin Haraldsson, Gesa Helmsorig, Venkatasubbu Thirulogachandar, Rüdiger Simon, Maria von Korff","doi":"10.1093/plphys/kiaf118","DOIUrl":"https://doi.org/10.1093/plphys/kiaf118","url":null,"abstract":"<p><p>High ambient temperature (HT) impairs reproductive development and grain yield in temperate crops. To ensure reproductive success under HT, plants must maintain developmental stability. However, the mechanisms integrating plant development and temperature resistance are largely unknown. Here, we demonstrate that PHOTOPERIOD 1 (PPD-H1), homologous to PSEUDO RESPONSE REGULATOR genes of the Arabidopsis (Arabidopsis thaliana) circadian clock, controls developmental stability in response to HT in barley (Hordeum vulgare). We analyzed the HT responses in independent introgression lines with either the ancestral wild-type Ppd-H1 allele or the natural ppd-h1 variant, selected in spring varieties to delay flowering and enhance yield under favorable conditions. HT delayed inflorescence development and reduced grain number in ppd-h1 mutant lines, while the wild-type Ppd-H1 genotypes exhibited accelerated reproductive development and showed a stable grain set under HT. Using a CRISPR/Cas9-induced ppd-h1 mutant, we confirmed that the CCT domain of Ppd-H1 controls developmental stability, but not clock gene expression. Transcriptome and phytohormone analyses in developing leaves and inflorescences revealed increased expression levels of stress-responsive genes and abscisic acid levels in the leaf and inflorescence of the natural and induced mutant ppd-h1 lines. Furthermore, the ppd-h1 lines displayed downregulated photosynthesis- and energy metabolism-related genes, as well as decreased auxin and cytokinin levels in the inflorescence, which impaired anther and pollen development. In contrast, the transcriptome, phytohormone levels, and anther and pollen development remained stable under HT in the wild-type Ppd-H1 plants. Our findings suggest that Ppd-H1 enhances stress resistance and energy metabolism, thereby stabilizing reproductive development, floret fertility and grain set under HT.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR164a-targeted NAM3 inhibits thermotolerance in tomato by regulating HSFA4b-mediated redox homeostasis.","authors":"Zelan Huang, Rui Lin, Yufei Dong, Mingjia Tang, Xiaojian Xia, Lei Fang, Jingquan Yu, Huijia Kang, Yanhong Zhou","doi":"10.1093/plphys/kiaf113","DOIUrl":"https://doi.org/10.1093/plphys/kiaf113","url":null,"abstract":"<p><p>Extreme weather events, including high temperatures, frequently occur and adversely affect crop growth, posing substantial challenges to global agriculture. MicroRNAs (miRNAs) play integral roles in regulating plant growth and responses to various stresses. In this study, we reveal that microRNA164a (miR164a) in tomato (Solanum lycopersicum) is a pivotal element that exhibits a rapid positive response to heat stress (HS) among multiple miRNAs, while its target NO APICAL MERISTEM 3 (NAM3) shows an opposite complementary response. MiR164a/b-5p-deficient mutant and NAM3-overexpressing plants resulted in increased sensitivity to HS, whereas mutants with reduced NAM3 levels exhibited enhanced thermotolerance. Importantly, HS-induced reactive oxygen species (ROS) accumulation and antioxidant enzyme activities were positively regulated by miR164a and negatively by NAM3, respectively. Furthermore, we demonstrated that NAM3 transcriptionally activated the expression of HSFA4b, and silencing HSFA4b improved tomato thermotolerance. HSFA4b repressed the expression of the antioxidant gene APX1 and the heat shock protein (HSP) gene HSP90, disrupting redox homeostasis and exacerbating oxidative stress. Our findings unveil a pivotal regulatory pathway governed by the miR164a-NAM3 module that confers thermotolerance in tomato via its influence on ROS-related and HSP pathways. These findings provide valuable insights into the molecular mechanisms that underpin tomato thermotolerance, which are crucial for advancing sustainable agricultural practices, particularly in the face of the challenges presented by global climate change.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}