Plant Physiology最新文献_第3页

Derlin-like proteins are essential for the function of Euglena gracilis chloroplasts. 木质素样蛋白是凤尾草叶绿体功能的重要组成部分。

IF 6.9 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag253

Anežka Konupková, Zoltán Füssy, Priscila Peña-Diaz, Masami Nakazawa, Vladimír Hampl

{"title":"Derlin-like proteins are essential for the function of Euglena gracilis chloroplasts.","authors":"Anežka Konupková, Zoltán Füssy, Priscila Peña-Diaz, Masami Nakazawa, Vladimír Hampl","doi":"10.1093/plphys/kiag253","DOIUrl":"https://doi.org/10.1093/plphys/kiag253","url":null,"abstract":"<p><p>Despite the growing interest in basic research on Euglena gracilis and its importance in bioproduction, several key questions regarding its cell biology remain unanswered. One of them concerns the mechanism of protein import into its secondary chloroplasts, which are derived from a green alga. In this study, we prove the essentiality of two rhomboid pseudoproteases, DerL-1 and DerL-2, which are predicted to localize in the membrane of the chloroplast envelope. We demonstrate that these proteins belong to a euglenid-specific subgroup unrelated to other eukaryotic rhomboid pseudoproteases. Silencing these proteins using RNA interference leads to a distinct phenotype marked by culture bleaching and delayed growth. This is accompanied by the loss of typical chloroplast structure and a reduction in the expression of a substantial portion of the chloroplast proteome, especially proteins involved in photosynthesis. We hypothesize that these pseudoproteases are involved in chloroplast protein import by forming a core component of the translocon across the middle envelope membrane. This indicates a remarkable case of molecular convergence, as other algae utilize rhomboid pseudoproteases-unrelated to euglenid proteins-for protein import into plastids derived from red algae.</p>","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147778159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maize XRCC2 participates in somatic DNA repair and meiotic crossover formation. 玉米XRCC2参与体细胞DNA修复和减数分裂交叉形成。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag255

Shuanghui Zhao,Liqun Chen,Jinghan Liu,Ting Zhang

{"title":"Maize XRCC2 participates in somatic DNA repair and meiotic crossover formation.","authors":"Shuanghui Zhao,Liqun Chen,Jinghan Liu,Ting Zhang","doi":"10.1093/plphys/kiag255","DOIUrl":"https://doi.org/10.1093/plphys/kiag255","url":null,"abstract":"The eukaryotic radiation sensitive 51 (RAD51) gene family comprises seven ancient paralogs that have been remarkably conserved across both plants and animals. Among these paralogs, X-ray repair cross complementing 2 (XRCC2) plays a pivotal role in mammalian embryonic development. However, in Arabidopsis (Arabidopsis thaliana), disruption of its function leads to seemingly normal meiotic processes, and intriguingly, even appears to increase meiotic recombination. Given these disparate observations, the precise role of XRCC2 during meiosis remains largely elusive. Here, we identified the XRCC2 gene in maize (Zea mays), a member of the RAD51-paralog family and the homolog of Arabidopsis XRCC2. Plants with mutated XRCC2 exhibited partial male and female sterility. Cytological investigations of xrcc2 mutants revealed that the meiocytes form univalent chromosomes and chromosome bridges and undergo chromosome fragmentation, while homologous pairing and synapsis occur normally. Notably, the xrcc2 mutant showed a significant reduction in the number of meiotic chiasmata and RAD51 foci. Furthermore, XRCC2 was essential for maintaining genome stability and affected the transcription of RAD51 paralogs. Within yeast and tobacco systems, we detected interactions between XRCC2 and disrupted meiotic cDNA1 (DMC1), Radiation sensitive 51C (RAD51C), and radiation sensitive 51D (RAD51D). Collectively, XRCC2 demonstrates remarkable functional diversity across species and plays an important role in maize crossover formation.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"12 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147754995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Don't make me blush: MsSPX4/MsPHL11-MsWRKY91 regulates anthocyanin synthesis in Malus spectabilis under low phosphorus stress. 别让我脸红：MsSPX4/MsPHL11-MsWRKY91调控低磷胁迫下苹果花青素合成。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-29 DOI: 10.1093/plphys/kiag167

Catherine Freed

引用次数: 0

A simple and reliable method for determining kanamycin sensitivity in Brassica napus. 一种简便可靠的测定甘蓝型油菜卡那霉素敏感性的方法。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-24 DOI: 10.1093/plphys/kiag244

Xuelin Wu,Alexandre Miaule,Joanne Chory

引用次数: 0

The small protein SbtC is a functional component of the CO2 concentrating mechanism in Synechocystis sp. PCC 6803. 小蛋白SbtC是Synechocystis sp. PCC 6803中CO2浓缩机制的一个功能组分。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-24 DOI: 10.1093/plphys/kiag246

Peter Walke,Carolin Poppitz,Niklas Menz,Wolfgang R Hess,Robert Burnap,Martin Hagemann,Stephan Klähn

{"title":"The small protein SbtC is a functional component of the CO2 concentrating mechanism in Synechocystis sp. PCC 6803.","authors":"Peter Walke,Carolin Poppitz,Niklas Menz,Wolfgang R Hess,Robert Burnap,Martin Hagemann,Stephan Klähn","doi":"10.1093/plphys/kiag246","DOIUrl":"https://doi.org/10.1093/plphys/kiag246","url":null,"abstract":"Oxygenic phototrophs fix CO2 via the enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), which shows relatively low CO2 affinity and specificity. To circumvent low and fluctuating CO2 concentrations in aquatic systems, cyanobacteria and algae have evolved sophisticated inorganic carbon (Ci) concentrating mechanisms (CCMs). Bicarbonate transporters such as SbtA play a crucial role in the cyanobacterial CCM and hence display multiple layers of tight regulation. Control of sbtA gene expression and corresponding transporter activity involves the PII-like protein SbtB, whose gene is frequently co-transcribed with sbtA. A previously non-annotated gene located upstream of the sbtAB operon in the model Synechocystis sp. PCC 6803 encodes the small protein SbtC, composed of 80 amino acids. Presence of SbtC was confirmed by immunoblotting of the sbtC-coding sequence fused to a Flag-tag. Similar to sbtAB, transcription of the sbtC locus is induced by low CO2 availability; however, it is controlled independently. Mutation of the sbtC locus in a wild-type background produced only a mild phenotype, even under low CO2, but impaired diurnal growth resembled that of the mutant ΔsbtB. Biochemical analysis indicated a trimeric SbtABC complex in the membrane. Bicarbonate leakage from cells was strongly elevated when either sbtB or sbtC was deleted from recombinant Synechocystis strains harboring only SbtA as single Ci uptake system. Our results provide evidence that SbtC contributes to the formation of the SbtAB complex, thereby regulating bicarbonate exchange at the cytoplasmic membrane. Well-conserved SbtC-like proteins encoded in the neighborhood of sbtAB exist in many cyanobacterial genomes, pointing towards an important role in the cyanobacterial CCM.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"20 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147739155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethylene Signaling in Roots: Insights into Gene Regulatory Networks. 乙烯信号在根：洞察基因调控网络。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-24 DOI: 10.1093/plphys/kiag240

Blanca Jazmin Reyes-Hernández

引用次数: 0

Two genes, one switch: a bidirectional promoter strategy for inducible plant immunity. 两个基因，一个开关：诱导植物免疫的双向启动子策略。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-24 DOI: 10.1093/plphys/kiag243

Benjamin J M Tremblay

引用次数: 0

Evolution of trans-isoprenyl diphosphate synthases in the plant kingdom. 植物界反式异戊二烯二磷酸合成酶的进化。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-22 DOI: 10.1093/plphys/kiag238

Xinlu Chen,Mutsumi Nakanishi,Jin Han,Hisashi Hemmi,Feng Chen

{"title":"Evolution of trans-isoprenyl diphosphate synthases in the plant kingdom.","authors":"Xinlu Chen,Mutsumi Nakanishi,Jin Han,Hisashi Hemmi,Feng Chen","doi":"10.1093/plphys/kiag238","DOIUrl":"https://doi.org/10.1093/plphys/kiag238","url":null,"abstract":"Terpenoid metabolism is essential across all life forms. Trans-isoprenyl diphosphate synthases (IDSs) are universally conserved enzymes that produce isoprenyl diphosphates of varying chain lengths, serving as precursors for diverse terpenoid metabolites. However, the evolution of IDS genes within the plant kingdom (Archaeplastida) remains unresolved. Here, we reconstruct the evolutionary trajectory of IDS genes in Archaeplastida using comprehensive genomic mining and phylogenetic analyses. We infer that the last common ancestor of the plant kingdom possessed four distinct IDS genes: two from the eukaryotic host encoding farnesyl diphosphate synthase (FPPS) and polyprenyl diphosphate synthase using farnesyl diphosphate as substrate (PPPSF), and two from the cyanobacterial endosymbiont encoding geranylgeranyl diphosphate synthase (GGPPS) and polyprenyl diphosphate synthase using geranylgeranyl diphosphate as a substrate (PPPSGG). While all the cyanobacterial-derived genes have been retained, the host-derived GGPPS was lost in the Archaeplastida ancestor. Most IDSs are nuclear-encoded, yet GGPPS in glaucophytes and PPPSGG in both glaucophytes and rhodophytes are plastid-encoded, indicating lineage-specific endosymbiotic gene transfer. One representative plastid-encoded PPPSGG shares catalytic properties with its nuclear-encoded counterparts. Among different lineages of the plant kingdom, the GGPPS subfamily is substantially expanded in vascular plants, consistent with the diversification of terpenoid metabolism in these later diverged lineages of land plants. Together, these findings reveal a dual origin and a complex evolutionary trajectory of IDS genes in the plant kingdom, shaped by endosymbiotic gene transfer, differential gene retention, and lineage-specific expansion.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"25 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147735202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the crosstalk between extracellular ATP and glutamate in systemic ROS signaling. 揭示细胞外ATP和谷氨酸在系统ROS信号传导中的串扰。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-22 DOI: 10.1093/plphys/kiag241

Hannah Rae Thomas

引用次数: 0

XAP5 CIRCADIAN TIMEKEEPER coordinates circadian rhythms and anthocyanin biosynthesis independently of splicing. XAP5 CIRCADIAN TIMEKEEPER独立于剪接协调昼夜节律和花青素的生物合成。

IF 7.4 1区生物学

Plant Physiology Pub Date : 2026-04-22 DOI: 10.1093/plphys/kiag235

Hongtao Zhang,Stacey L Harmer

{"title":"XAP5 CIRCADIAN TIMEKEEPER coordinates circadian rhythms and anthocyanin biosynthesis independently of splicing.","authors":"Hongtao Zhang,Stacey L Harmer","doi":"10.1093/plphys/kiag235","DOIUrl":"https://doi.org/10.1093/plphys/kiag235","url":null,"abstract":"Circadian clocks provide plants with an adaptive advantage by enabling them to anticipate daily environmental changes. The periodicity of circadian clocks is regulated at multiple levels of gene expression, including transcription, mRNA processing, translation, and protein modification. Numerous mRNA splicing factors have been implicated in maintaining circadian period length. However, these factors often play additional roles in transcription, making it difficult to determine whether they affect the clock through splicing-dependent or -independent mechanisms. We and others have shown that XAP5 CIRCADIAN TIMEKEEPER (XCT) and components of the PRE-MRNA-PROCESSING FACTOR 19 (PRP19) complex, including the functionally redundant PRP19A and PRP19B, physically associate and regulate both splicing and circadian rhythms. Here, our transcriptome analyses reveal that the antagonistic regulation of circadian period length by XCT and PRP19 likely occurs through splicing-independent mechanisms. Interestingly, both factors co-regulate expression of a substantial set of shared target genes involved in RNA metabolism, photosynthesis, and stress responses despite having largely distinct targets for splicing. Gene co-expression analysis followed by functional characterization identified anthocyanin biosynthesis as another process antagonistically regulated by XCT and PRP19. Nonetheless, we found genetic perturbation of anthocyanin production does not affect circadian period, suggesting that the observed correlation between anthocyanin levels and circadian period may instead reflect disruption of a shared upstream regulatory pathway. Together, our findings suggest involvement of XCT and PRP19 in the transcriptional coordination of anthocyanin biosynthesis and biological timing, expanding their known roles beyond mRNA splicing.","PeriodicalId":20101,"journal":{"name":"Plant Physiology","volume":"30 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147731362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0