Junyi Zhan, Haixia Wang, Mengyang Niu, Changjian Bao, Yanning Gong, Gang Hu, Dongqing Xu, Zhenguo Shen, Nana Su
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引用次数: 0
Abstract
Soybean (Glycine max) accumulates a substantial amount of flavonoids, including anthocyanins and flavonols, which play essential roles in both plant growth and human health. While blue light promotes flavonoid biosynthesis, the regulatory circuitry governing this process remains poorly characterized. Here, we demonstrate that blue light stimulates flavonol and anthocyanin accumulation in soybean hypocotyls by inducing the B-box transcription factor GmBBX22. Mechanistically, GmBBX22 transcripts are alternatively spliced under blue light, generating three isoforms (designated as X1, X2, and X3). Intriguingly, the truncated isoforms X2 and X3 induce flavonol and anthocyanin biosynthesis through distinct regulatory cascades. Rather than directly targeting the biosynthesis flavonol synthase gene (GmFLS) or dihydroflavonol 4-reductase gene (GmDFR), which encode key enzymes for metabolic flux partitioning between flavonols and anthocyanins, X2 and X3 specifically activate the expression of GmMYB12 and GmMYB90, respectively. Subsequent functional analyses revealed that these MYB transcription factors induce GmFLS and GmDFR expression to coordinate flavonol and anthocyanin accumulation. Collectively, our work delineates a splicing-dependent regulatory module where GmBBX22 isoforms mediate light quality adaptation in soybean through divergent transcriptional programming of flavonoid metabolism.
期刊介绍:
Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research.
As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.