B-BOX22 isoforms activate expression of MYBs and elicit blue light-induced flavonoid biosynthesis in soybean.

IF 6.5 1区 生物学 Q1 PLANT SCIENCES
Junyi Zhan, Haixia Wang, Mengyang Niu, Changjian Bao, Yanning Gong, Gang Hu, Dongqing Xu, Zhenguo Shen, Nana Su
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引用次数: 0

Abstract

Soybean (Glycine max) accumulates a substantial amount of flavonoids, including anthocyanins and flavonols, which play essential roles in both plant growth and human health. While blue light promotes flavonoid biosynthesis, the regulatory circuitry governing this process remains poorly characterized. Here, we demonstrate that blue light stimulates flavonol and anthocyanin accumulation in soybean hypocotyls by inducing the B-box transcription factor GmBBX22. Mechanistically, GmBBX22 transcripts are alternatively spliced under blue light, generating three isoforms (designated as X1, X2, and X3). Intriguingly, the truncated isoforms X2 and X3 induce flavonol and anthocyanin biosynthesis through distinct regulatory cascades. Rather than directly targeting the biosynthesis flavonol synthase gene (GmFLS) or dihydroflavonol 4-reductase gene (GmDFR), which encode key enzymes for metabolic flux partitioning between flavonols and anthocyanins, X2 and X3 specifically activate the expression of GmMYB12 and GmMYB90, respectively. Subsequent functional analyses revealed that these MYB transcription factors induce GmFLS and GmDFR expression to coordinate flavonol and anthocyanin accumulation. Collectively, our work delineates a splicing-dependent regulatory module where GmBBX22 isoforms mediate light quality adaptation in soybean through divergent transcriptional programming of flavonoid metabolism.

B-BOX22亚型激活MYBs的表达,诱导蓝光诱导的大豆类黄酮生物合成。
大豆(Glycine max)积累了大量的黄酮类化合物,包括花青素和黄酮醇,对植物生长和人体健康都起着至关重要的作用。虽然蓝光促进类黄酮的生物合成,但控制这一过程的调控电路仍然缺乏特征。在这里,我们证明蓝光通过诱导B-box转录因子GmBBX22刺激黄酮醇和花青素在大豆下胚轴中的积累。从机制上讲,GmBBX22转录本在蓝光下交替剪接,产生三种异构体(指定为X1, X2和X3)。有趣的是,截断的X2和X3通过不同的调节级联诱导黄酮醇和花青素的生物合成。X2和X3不是直接靶向生物合成黄酮醇合成酶基因(GmFLS)或二氢黄酮醇4-还原酶基因(GmDFR),它们编码黄酮醇和花青素代谢通量分配的关键酶,而是分别特异性激活GmMYB12和GmMYB90的表达。随后的功能分析表明,这些MYB转录因子诱导GmFLS和GmDFR表达,协调黄酮醇和花青素的积累。总的来说,我们的工作描绘了一个剪接依赖的调节模块,其中GmBBX22同工型通过类黄酮代谢的不同转录编程介导大豆的光质适应。
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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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