The transcriptional repressors IAA5 and IAA29 participate in DNA damage-induced stem cell death in Arabidopsis roots.

IF 6.5 1区 生物学 Q1 PLANT SCIENCES
Naoki Takahashi, Nobuo Ogita, Toshiya Koike, Kohei Nishimura, Soichi Inagaki, Ye Zhang, Takumi Higaki, Masaaki Umeda
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引用次数: 0

Abstract

Plants generate organs continuously during post-embryonic development. Thus, their ability to preserve stem cells in changing environments is crucial for their survival. Genotoxic stress threatens genome stability in all somatic cells. However, in the meristem, only the stem cells actively die in response to DNA damage, followed by stem cell replenishment that guarantees genome stability in these cells. Cytokinin biosynthesis-induced inhibition of downward auxin flow participates in DNA damage-induced stem cell death in roots. Without this system, stem cell death occurs at a reduced but significant level, suggesting another mechanism governing the DNA damage response in stem cells. Here, we demonstrate that in response to DNA double-strand breaks, the AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) family members IAA5 and IAA29, encoding negative regulators of auxin signaling, are induced in Arabidopsis (Arabidopsis thaliana) roots. The transcription factor SUPPRESSOR OF GAMMA RESPONSE 1 directly induces their expression as an active response to DNA damage. In the iaa5 iaa29 double mutant, DNA damage-induced stem cell death is greatly suppressed, while it is fully restored by the expression of a stable form of IAA5 in vascular stem cells. Our genetic data reveal that reduced auxin signaling around the stem cell niche, caused by IAA5 and IAA29 induction and enhanced cytokinin biosynthesis, is a prerequisite for cell death induction, thus playing a central role in maintaining genome integrity in root stem cells.

转录抑制因子IAA5和IAA29参与拟南芥根系DNA损伤诱导的干细胞死亡。
植物在胚胎后发育过程中不断产生器官。因此,它们在不断变化的环境中保存干细胞的能力对它们的生存至关重要。基因毒性应激威胁着所有体细胞的基因组稳定性。然而,在分生组织中,只有干细胞因DNA损伤而主动死亡,随后是干细胞补充,以保证这些细胞的基因组稳定性。细胞分裂素生物合成诱导的生长素向下流动的抑制参与了DNA损伤诱导的根干细胞死亡。如果没有这个系统,干细胞的死亡发生在较低但显著的水平上,这表明在干细胞中控制DNA损伤反应的另一种机制。在这里,我们证明了响应DNA双链断裂,在拟南芥(拟南芥)根中,编码生长素信号负调控因子的生长素/吲哚-3-乙酸(Aux/IAA)家族成员IAA5和IAA29被诱导。转录因子SUPPRESSOR OF GAMMA RESPONSE 1直接诱导其表达,作为对DNA损伤的主动反应。在iaa5 iaa29双突变体中,DNA损伤诱导的干细胞死亡被极大地抑制,而通过在血管干细胞中表达一种稳定形式的iaa5而完全恢复。我们的遗传数据显示,IAA5和IAA29诱导导致干细胞生态位周围生长素信号的减少,以及细胞分裂素生物合成的增强,是细胞死亡诱导的先决条件,因此在维持根干细胞基因组完整性中起着核心作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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