肽激素RGF1通过活性氧依赖的半胱氨酸残基调节PLETHORA2的稳定性。

IF 6.5 1区 生物学 Q1 PLANT SCIENCES
Yu-Chun Hsiao, Shiau-Yu Shiue, Ming-Ren Yen, Joon-Keat Lai, Masashi Yamada
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引用次数: 0

摘要

根分生组织生长因子1 (RGF1)肽通过改变根发育区域中超氧化物(O2-)和过氧化氢(H2O2)的不同定位来扩展PLETHORA2 (PLT2)蛋白梯度。然而,活性氧(ROS)调控PLT2稳定性的潜在机制尚不清楚。在这里,我们证明了PLT2的212半胱氨酸是通过ROS调节PLT2稳定性的关键。在H2O2积累开始的伸长区,PLT2蛋白浓度梯度迅速降低。然而,用丝氨酸(PLT2C212S)取代PLT2的第212半胱氨酸后,PLT2在延伸区更稳定,RGF1定位范围更广,对H2O2的抗性更强。高浓度H2O2处理后,检测到PLT2的亚砜化,表明第212半胱氨酸的s -亚砜化通过局部ROS分布控制PLT2蛋白的稳定性。这些发现表明,通过ROS形成PLT2浓度梯度依赖于涉及212半胱氨酸的PLT2亚砜化机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The peptide hormone RGF1 modulates PLETHORA2 stability via reactive oxygen species-dependent regulation of a cysteine residue.

The Root meristem growth factor 1 (RGF1) peptide extends the PLETHORA2 (PLT2) protein gradient by altering the distinct localization of superoxide (O2-) and hydrogen peroxide (H2O2) among the root developmental zones. However, the underlying mechanism through which reactive oxygen species (ROS) regulate PLT2 stability is unclear. Here, we demonstrate that the 212th cysteine of PLT2 is pivotal in modulating PLT2 stability through ROS. The PLT2 protein concentration gradient rapidly decreases in the elongation zone, where H2O2 accumulation initiates. However, substituting the 212th cysteine of PLT2 with serine (PLT2C212S) results in PLT2 being more stable in the elongation zone, more broadly localized by RGF1, and showing robust resistance to H2O2. The sulfenylation of PLT2 was detected following treatment with H2O2 at high concentrations, suggesting that S-sulfenylation of the 212th cysteine controls PLT2 protein stability through local ROS distributions. These findings show that the formation of the PLT2 concentration gradient through ROS depends on a PLT2 sulfenylation mechanism that involves the 212th cysteine.

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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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