Pesticide Biochemistry and Physiology最新文献

{"title":"Pyroxasulfone tolerance in wheat: Role of enhanced herbicide metabolism and coexpression of key metabolic genes","authors":"Ruosheng Yang ,&nbsp;Hongle Xu ,&nbsp;Qinghao Liu ,&nbsp;Lanlan Sun ,&nbsp;Wangcang Su ,&nbsp;Fei Xue ,&nbsp;Renhai Wu","doi":"10.1016/j.pestbp.2025.106691","DOIUrl":"10.1016/j.pestbp.2025.106691","url":null,"abstract":"<div><div>Pyroxasulfone has been widely used to control malignant weeds in wheat fields. However, differences in pyroxasulfone tolerance across wheat varieties warrant attention owing to their potential impact on food production security. In this study, 54 wheat varieties were collected for screening, and Yunong 922 with 6.01-fold higher tolerance than Zhengmai 1354 was identified. Ultra-performance liquid chromatography–mass spectrometry analysis revealed that Yunong 922 exhibited a significantly shorter degradation half-life of pyroxasulfone (5.03 days) than Zhengmai 1354 (7.02 days). The P450 inhibitor malathion and the glutathione S-transferase (GST) inhibitor NBD-Cl reduced the tolerance factor of Yunong 922 to pyroxasulfone from 6.01 to 1.44 and 1.16, respectively, with no significant difference in tolerance to pyroxasulfone compared with Zhengmai 1354. RNA-Seq transcriptome analysis was used to identify candidate genes that may confer metabolic tolerance to pyroxasulfone in wheat. Eight candidate genes (five P450 enzymes and three GSTs) exhibited significantly different expression levels between Yunong 922 and Zhengmai 1354, as validated by qRT–PCR. Pyroxasulfone tolerance in Yunong 922 is associated with the coordinated upregulation of <em>CYP72A397</em>, <em>CYP72A14</em>, and <em>GSTDHAR1</em>, each contributing approximately 33.7 %, 32.6 %, and 33.7 % to the dominant principal component 1 that accounts for 88.7 % of the explained variance. These results revealed that the faster degradation of pyroxasulfone observed in Yunong 922 could be linked to the higher expression of P450s and GSTs, though this relationship remains to be confirmed. This study also offers valuable insights into mechanisms underlying crop tolerance and informs the development of herbicide management strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106691"},"PeriodicalIF":4.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics of susceptible and insecticide-resistant strains of Spodoptera frugiperda (Lepidoptera: Noctuidae) and metatranscriptomics of their midgut-associated microbiota","authors":"Ana Flávia Freitas Gomes ,&nbsp;Thaís Regiani Cataldi ,&nbsp;Carlos Alberto Labate ,&nbsp;Celso Omoto ,&nbsp;Fernando Luis Cônsoli","doi":"10.1016/j.pestbp.2025.106697","DOIUrl":"10.1016/j.pestbp.2025.106697","url":null,"abstract":"<div><div>The gut microbiota of insects can influence host physiology and, like the host, can be affected by stressors. In this study, we investigated the effects of insecticide exposure on the metabolome and gut microbial metatranscriptome of insecticide resistant and susceptible <em>Spodoptera frugiperda</em> larvae. Metabolomic analysis revealed distinct metabolic profiles influenced by host phenotype and treatment with three insecticides: flubendiamide, spinosad and teflubenzuron. Amino acids, monosaccharides and fatty acids were prominent among the differentially abundant metabolites. Insecticide exposure altered the interactions between the host and its microbiota in an insecticide-dependent manner. Phenotype emerged as an important factor influencing the metabolic profile of <em>S. frugiperda</em> in response to insecticide exposure. For certain metabolites, this influence is observed constitutively, independent of treatment. This study highlights the role of members of the gut microbiota contributing with metabolites involved in host detoxification. In addition, it demonstrates that insecticide exposure and resistance evolution may alter host immunity and its interaction with the gut microbial community. Our findings help to clarify the complex interplay between insecticide exposure, gut microbiota, and host response, and provide insights into the dynamics of the gut microbiota of <em>S. frugiperda</em> in response to insecticides.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106697"},"PeriodicalIF":4.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and synthesis of aminol- and phenylamide-containing phosphonic acids and their biological activity evaluation","authors":"Zili Yang,&nbsp;Xin Chen,&nbsp;Liru Zheng,&nbsp;Qinping Hu,&nbsp;Mengwei Bu,&nbsp;Jiang Duan,&nbsp;Aidong Zhang","doi":"10.1016/j.pestbp.2025.106690","DOIUrl":"10.1016/j.pestbp.2025.106690","url":null,"abstract":"<div><div>Inhibitor design based on the natural product fosmidomycin (FOS) as a lead structure is a promising strategy in search of herbicidally active compounds that may target the DXR enzyme in the MEP pathway. Herein, two metal ion chelating groups, aminol and amide, were used to replace the hydroxamate moiety of FOS, and two types of non-hydroxamate FOS analogs, namely aminol- (<strong>5a</strong> <strong>∼</strong> <strong>n</strong> and <strong>6a</strong> <strong>∼</strong> <strong>o</strong>) and phenylamide-containing (<strong>10a</strong> <strong>∼</strong> <strong>v</strong>) phosphonic acids were designed and synthesized by establishing facile routes involving the Michaelis-Arbuzov reaction, epoxide ring-opening, acrylamidation, Michael addition, and phosphonate hydrolysis. In the pre-emergency bioactivity screening several compounds demonstrated herbicidal activities superior to FOS, including <strong>5c</strong>, <strong>5</strong> <strong>h</strong>, <strong>10a</strong>, <strong>10d</strong>, <strong>10e</strong>, <em>etc.</em> In particular, <strong>10e</strong> showed an 18.7-fold inhibition activity against <em>Arabidopsis thaliana</em> and 16.1- and 10.8-fold activities against the root and stalk of <em>Echinochloa crus-galli</em>, respectively, in comparison with that of FOS. In the post-emergency assay, <strong>10e</strong> also displayed 1.9- and 2.1-fold inhibition activities against <em>E. crus-galli</em> and <em>Amaranthus retroflexus</em>. Enzyme inhibition assay revealed the inhibition activities of some compounds to the DXR enzyme far below that of FOS, while the plant growth rescue failed from the inhibition by <strong>5c</strong> and <strong>10e</strong> by adding dimethylallyl pyrophosphate (DMAPP), a downstream product of the MEP pathway, both suggesting that some targets instead of the DXR enzyme might be involved in the inhibition by the active compounds. Nevertheless, this work demonstrated that using bioisosteric replacement based on the lead structure FOS is an effective strategy for developing highly herbicidal active compounds.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106690"},"PeriodicalIF":4.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fitness cost and molecular regulation analysis of thifluzamide resistance in Rhizoctonia solani AG-5 inciting maize banded leaf and sheath blight in Shanxi Province of China","authors":"Xiaojing Shi ,&nbsp;Yuting Li ,&nbsp;Naibo Yang ,&nbsp;Shuhui Fu ,&nbsp;Yanhua Xin ,&nbsp;He Zhang","doi":"10.1016/j.pestbp.2025.106696","DOIUrl":"10.1016/j.pestbp.2025.106696","url":null,"abstract":"<div><div><em>Rhizoctonia solani</em> anastomosis group (AG)-5 is the predominant pathogen inciting maize banded leaf and sheath blight in Shanxi province, China, for which the sensitivity baseline to thifluzamide has been established. However, no risk assessment of thifluzamide resistance in <em>R. solani</em> AG-5 has been conducted. Consequently, thifluzamide-resistant mutants of <em>R. solani</em> AG-5 were generated by combined UV irradiation and fungicide selection in the laboratory. Fitness, genetic stability, intracellular structures, and fitness-related differentially expressed genes were subsequently analyzed. Ten resistant mutants were obtained, all of which exhibited moderate-to-high resistance that remained stable for 20 generations. These mutants showed a subtle expansion in their optimal growth temperature and pH ranges as their resistance to thifluzamide increased. Despite this, they exhibited reduced mycelial growth, sclerotia production, melanin formation, and virulence, whereas sclerotia germination rates were higher than in the parental sensitive strain. The mutant Y94.4R maintained vacuole numbers and size post-thifluzamide treatment, whereas the sensitive strain Y94 exhibited vacuole disappearance. Compared to Y94 strain, the expression levels of <em>Atg22</em>, <em>Vps45</em>, <em>Ktr1_3</em>, <em>Aha1</em>, <em>DnaJ</em>, Hikeshi, and <em>Stip1</em> gene were less upregulated (1.81–3.65 folds) in Y94.4R strain under thifluzamide stress. No amino-acid substitutions were detected in SdhA, SdhB, and SdhD subunits; however, alanine (A) at position 46 of SdhC subunit was replaced by threonine (T) in thifuzamide-resistant mutants. This study showed that the resistant mutants exhibited reduced ecological fitness and competitiveness, suggested that the observed fitness costs might partially support the durability of thifluzamide under field conditions, and provided candidate genes for future disease-management strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106696"},"PeriodicalIF":4.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal activity and function mechanisms of chitooligosaccharide against Gymnosporangium pleoporum, the pathogen causing rust in Juniperus przewalskii","authors":"Junchi Zhao ,&nbsp;Jiahui Jiao ,&nbsp;Taijun Fang ,&nbsp;Hailan Li ,&nbsp;Luchao Bai ,&nbsp;Peiqin Li","doi":"10.1016/j.pestbp.2025.106685","DOIUrl":"10.1016/j.pestbp.2025.106685","url":null,"abstract":"<div><div><em>Juniperus przewalskii</em>, a keystone species in China's ecologically fragile Sanjiangyuan region, faces severe threats from <em>Gymnosporangium pleoporum</em> rust. This study establishes chitooligosaccharides (COS) as potent antifungal agents against <em>G. pleoporum</em>, dose-dependently suppressing teliospore germination and viability. Integrated physiological analyses revealed COS-induced severe membrane damage evidenced by electrolyte leakage, soluble protein efflux, SEM-confirmed structural deformities, and elevated malondialdehyde indicating lipid peroxidation. Concurrently, COS triggered oxidative catastrophe via reactive oxygen species accumulation with suppression of antioxidant enzymes, while collapsing energy metabolism through adenosine triphosphate depletion and inhibition of electron transport chain enzymes. Transcriptomics identified concentration-dependent differential expression in energy metabolism pathways (glycolysis, oxidative phosphorylation, fatty acid degradation), alongside disrupted protein synthesis and redox homeostasis. Crucially, nine viability-correlated downregulated core genes, including putative orthologs for oxidative defense and aromatic amino acid biosynthesis, exhibited lineage-specific functions. Collectively, COS acts as a multi-target antifungal agent directly disrupting membrane integrity, redox homeostasis, and energy / protein metabolism in rust fungi, distinct from plant-induced resistance. This study establishes the scientific foundation for COS deployment against <em>J. przewalskii</em> rust disease, highlighting its eco-compatible potential through targeted exploitation of essential pathogen vulnerabilities. Furthermore, it positions COS as a cornerstone for precision forestry therapeutics that concurrently achieve pathogen suppression and ecological integrity preservation in vulnerable montane ecosystems.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106685"},"PeriodicalIF":4.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Penicillic acid-mediated quorum-sensing inhibition suppresses motility and virulence of Xanthomonas albilineans","authors":"Meilin Li ,&nbsp;Cuilin Huang ,&nbsp;Liya Xiong ,&nbsp;Xiao Yang ,&nbsp;Charles A. Powell ,&nbsp;Ronghui Wen ,&nbsp;Muqing Zhang","doi":"10.1016/j.pestbp.2025.106664","DOIUrl":"10.1016/j.pestbp.2025.106664","url":null,"abstract":"<div><div>Sugarcane leaf scald, caused by <em>Xanthomonas albilineans</em>, is a significant bacterial disease that poses a substantial threat to global sugar production. The quorum-sensing (QS) system in <em>X. albilineans</em> presents a promising target for anti-virulence strategies. Here, we demonstrate that penicillic acid exhibits potent antibacterial activity against <em>X. albilineans</em>, with a minimum inhibitory concentration (MIC) of 6 μg/mL and an effective concentration for 50 % inhibition (EC₅₀) of 3.703 μg/mL. Transcriptome analysis, molecular docking, and surface plasmon resonance (SPR) confirmed that penicillic acid competitively binds the sensory histidine kinase RpfC, disrupting DSF-mediated QS signaling. At sub-inhibitory concentrations (½ MIC), penicillic acid significantly suppressed twitching, swimming, and swarming motility by downregulating genes associated with type IV pili, flagellar assembly, and chemotaxis. At full MIC, direct binding of penicillic acid to the FlgE and CheW proteins further impaired motility. These findings elucidate the molecular mechanism of penicillic acid and support its potential as a quorum-sensing inhibitor for biocontrol of sugarcane leaf scald.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106664"},"PeriodicalIF":4.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome editing of detoxification gene repertoires in insects using clustered regularly interspaced short palindromic repeats (CRISPR): A systematic review and meta-analysis","authors":"Vinithashri Gautam,&nbsp;Suresh R. Jambagi,&nbsp;Mohan Muthugounder","doi":"10.1016/j.pestbp.2025.106687","DOIUrl":"10.1016/j.pestbp.2025.106687","url":null,"abstract":"<div><div>Decoding the molecular mechanisms underlying insect resistance to insecticides and host plant adaptation is essential for effective and sustainable Insecticide Resistance Management (IRM). Reverse genetic approaches targeting “target site resistance” can help control pests without harming pollinators and beneficial biocontrol agents. Understanding the role of mutations involved in xenobiotic resistance enables the judicious use of pesticides. CRISPR-based genome editing allows precise manipulation of detoxification genes, helping to decipher their roles in resistance development. This review provides a comprehensive overview of CRISPR-mediated genome editing in insect detoxification genes and their involvement in resistance mechanisms. In addition to synthesizing overall data trajectories, we present study-level effect sizes that highlight context-specific responses to gene editing, offering insights that can inform future experimental designs and functional validation studies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106687"},"PeriodicalIF":4.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mikanolide enhances tobacco TMV resistance by strengthening cell walls via EXP/PG repression and activating defense via LYKs","authors":"Taijiao Yang ,&nbsp;Wei Zhang ,&nbsp;Jinkang Qi ,&nbsp;Jinfang Li ,&nbsp;Zhuoqing Zhang ,&nbsp;Minghai Tian ,&nbsp;Qing Li ,&nbsp;Shijun Hu ,&nbsp;Xiaohui Yan","doi":"10.1016/j.pestbp.2025.106689","DOIUrl":"10.1016/j.pestbp.2025.106689","url":null,"abstract":"<div><div>Tobacco mosaic virus (TMV) is a major pathogen of Solanaceae, threatening tobacco yield and quality. In this study, 12 compounds from <em>Mikania micrantha</em> were evaluated for their inhibitory effects on TMV using the half-leaf method and ID-ELISA assay. Compound <strong>1</strong> (Mikanolide) showed superior protective (63.43 %) and therapeutic (69.09 %) activities compared to other compounds and Ningnanmycin. It also exhibited inactivation (50.98 %) and proliferation inhibition (60.64 %) against TMV. Molecular docking revealed its binding to TMV-CP through hydrogen and hydrophobic bonds. Mechanistic analyses indicated that Mikanolide elevated chlorophyll contents, flavonoid and total phenol biosynthesis, and salicylic acid accumulation, thereby enhancing defense-related enzymatic activity. Transcriptome analyses showed increased expression of cell wall-related <em>EXP</em> and <em>PG</em> genes, as well as <em>LYKs</em> defense response genes, while suppressing <em>LRR protein 66</em>, <em>Hsc70</em>, <em>BTB/POZ-TAZ</em>, polygalacturonase, and expansins, indicating maintenance of cell wall integrity during TMV infection. RT-qPCR confirmed these results. In conclusion, Mikanolide enhances tobacco's resistance to TMV by inhibiting viral replication and proliferation and activating the plant's disease resistance signaling.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106689"},"PeriodicalIF":4.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cleavage of dsRNA by StaufenC into siRNA affects the RNAi efficiency in the 28-spotted ladybeetle, Henosepilachna vigintioctopunctata","authors":"Zhaoyang Li ,&nbsp;Ziqi Cheng ,&nbsp;June-Sun Yoon ,&nbsp;Chunxiao Yang ,&nbsp;Fei Li ,&nbsp;Xuguo Zhou ,&nbsp;Youjun Zhang ,&nbsp;Huipeng Pan","doi":"10.1016/j.pestbp.2025.106688","DOIUrl":"10.1016/j.pestbp.2025.106688","url":null,"abstract":"<div><div>The efficacy of RNA interference (RNAi) in insects hinges critically on the precise conversion of double-stranded RNA (dsRNA) into small interfering RNAs (siRNAs), which are essential for initiating effective gene silencing. While our recent work identified <em>HvStaufenC</em> as a key regulator of RNAi efficiency in the coleopteran pest <em>Henosepilachna vigintioctopunctata</em>, the underlying mechanism remained unknown. To define <em>HvStaufenC</em>'s role, we employed complementary <em>in vitro</em> and <em>in vivo</em> strategies. <em>In vitro</em>, recombinant HvStaufenC protein efficiently processed dsRNA into siRNAs primarily under 35 nucleotides (nt) in length. Crucially, this catalytic activity was completely abolished in a mutant protein harboring disruptions in its essential LNN motif (HvStaufenC-Mutant). <em>In vivo</em>, small RNA sequencing of larvae injected with dsRNA targeting <em>HvTH</em> gene revealed striking differences: wild-type produced siRNAs overwhelmingly enriched for 21-nt species (49.08 % of 19–25-nt siRNAs), characterized by 5’-GAU/UUG/GAA and 3’-UUU/GUU/UUG cleavage motifs. In contrast, <em>HvStaufenC</em> knockout mutants (HvStaufenCKO) showed significantly impaired 21-nt siRNA generation (22.73 %) and exhibited altered cleavage preferences (5’-UUC/GAU/AUU; 3′-GAG/UAC/GGA). Functional validation confirmed the biological significance of these processing differences. Chimeric dsRNA constructs incorporating most abundant wild-type-derived 21-nt siRNA sequences triggered significantly stronger RNAi effects compared to constructs based on sequences derived from HvStaufenCKO mutants. Moreover, no phenotypic changes were observed in HvStaufenCKO 3rd instar larvae following the injection of chimeric dsRNAs. Collectively, these findings demonstrate that <em>HvStaufenC</em> is indispensable for efficient RNAi in <em>H. vigintioctopunctata</em>. It specifically mediates dsRNA cleavage to generate functional 21-nt siRNAs with distinct sequence motifs, providing novel mechanistic insights into RNAi regulation within insects.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106688"},"PeriodicalIF":4.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and validation of ABCB transporter genes involved in chlorantraniliprole and emamectin benzoate tolerance in Spodoptera exigua","authors":"Chunmei You ,&nbsp;Ruiguan Duan ,&nbsp;Yijuan Chen ,&nbsp;Ying Liu ,&nbsp;Xiangyun Ji","doi":"10.1016/j.pestbp.2025.106686","DOIUrl":"10.1016/j.pestbp.2025.106686","url":null,"abstract":"<div><div><em>Spodoptera exigua</em> is a significant invasive pest that has developed resistance to various commonly applied insecticides. The detoxification of insecticides in insects may partially depend on ATP-binding cassette (ABC) transporters. Although the functions of <em>ABCB</em> subfamily genes are diverse in insects, the specific roles of <em>ABCBs</em> in <em>S. exigua</em> remain largely unexplored. This research focused on identifying ten <em>SeABCB</em> genes and characterizing their expression profiles across various developmental stages and tissues, as well as in response to different insecticide treatments. Our findings indicate that <em>SeABCB</em> genes are expressed at all developmental stages, with most ABC transporters exhibiting higher expression levels during the pupal and adult stages compared to the larval stage. Furthermore, high expression levels of <em>SeABCBs</em> were observed in diverse tissues: <em>SeABCB1</em> and <em>SeABCB6</em> were found to be more abundant in the midgut, while <em>SeABCB3</em> and <em>SeABCB4</em> exhibited significantly higher expression in fat bodies. The expression of <em>SeABCB2</em>, <em>SeABCB5</em> and <em>SeABCB8</em> was notable in Malpighian tubules, and <em>SeABCB9</em> showed a higher expression level in the cuticle than in other tissues. Eight <em>ABCBs</em> were upregulated in <em>S. exigua</em> following exposure to chlorantraniliprole and emamectin benzoate. Gene functional analysis based on RNAi proved that <em>SeABCB4</em> and <em>SeABCB9</em> are involved in the tolerance of <em>S. exigua to</em> chlorantraniliprole, while <em>SeABCB1</em> and <em>SeABCB9</em> participate in the transport or metabolism of emamectin benzoate in <em>S. exigua</em>. In summary, our research provides significant insights into the role of <em>SeABCBs</em> in the detoxification mechanisms of <em>S. exigua</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106686"},"PeriodicalIF":4.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}