Pesticide Biochemistry and Physiology最新文献

{"title":"Insecticide and pathogens co-exposure induces histomorphology changes in midgut and energy metabolism disorders on Apis mellifera","authors":"Yuxin Kang ,&nbsp;Junxiu Guo ,&nbsp;Tong Wu ,&nbsp;Bo Han ,&nbsp;Feng Liu ,&nbsp;Yu Chu ,&nbsp;Qiang Wang ,&nbsp;Jing Gao ,&nbsp;Pingli Dai","doi":"10.1016/j.pestbp.2025.106414","DOIUrl":"10.1016/j.pestbp.2025.106414","url":null,"abstract":"<div><div>Honey bees in agroecosystems face increasingly exposure to multiple stressors, such as pesticides and pathogens, making it crucial to assess their combined impacts rather than focusing on individual factors alone. This study examined the adverse effects of single exposure acetamiprid, <em>Varroa destructor</em>, and <em>Nosema ceranae</em>, both individually and in combination, on honey bee survival, midgut integrity and transcriptomic changes to understand the molecular mechanisms involved. The findings revealed that combination of acetamiprid and <em>N. ceranae</em> induced significant energetic stress, as evidenced by disruptions in energy metabolism. The synergistic effects of <em>V. destructor</em> and <em>N. ceranae</em> led to severe alterations in midgut histomorphology, particularly damaging the midgut epithelium. Concurrent exposure to acetamiprid and <em>V. destructor</em> inhibited the immune response and energy metabolism of honey bees, thereby exacerbating the vulnerability to pathogens and destabilizing their physiological equilibrium. The combination of all three stressors caused the most dramatic damage, disrupting midgut structure as well as aromatic amino acids and lipid metabolism. Our study underscored the complexity and unpredictability of stressor interactions, emphasizing the need to consider environmental context when assessing the risks of honey bee health.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106414"},"PeriodicalIF":4.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the biodegradation activity of Priestia aryabhattai 1–3I, a promising chlorpyrifos-degrading strain isolated from a local phosphogypsum landfill","authors":"Abdulsamie Hanano ,&nbsp;Nour Moursel ,&nbsp;Muhammad Hassan Obeid","doi":"10.1016/j.pestbp.2025.106416","DOIUrl":"10.1016/j.pestbp.2025.106416","url":null,"abstract":"<div><div>The current study emphasizes the potential of the microbial community within phosphogypsum landfills to identify promising microorganisms involved in Chlorpyrifos (CP) biodegradation, an organophosphorus pesticide extensively employed in the agricultural sector. We isolated 26 bacterial strains from CP-enriched phosphogypsum sample contaminated with 100 mg Kg⁻¹ CP and subsequently identified them through <em>16S rRNA</em> sequencing. Among these isolates, <em>Priestia aryabhattai</em> 1–3I displayed remarkable proficiency in utilizing CP as a sole carbon source. Furthermore, <em>P. aryabhattai</em> 1–3I was found to harbor an <em>oph</em>-encoding gene, a crucial component in the CP degradation pathway, with a highly conserved 694 bp region shared by at least 24 homologous <em>oph</em> bacterial genes. The CP-degrading ability of <em>P. aryabhattai</em> 1–3I was assessed both in liquid medium and soil samples, achieving degradation rates of 95 % and 60 %, respectively, starting from an initial concentration of 100 mg L⁻¹ CP after 4 weeks. This pronounced CP-degrading activity correlated with a rapid and significant increase in <em>oph</em> transcripts and was accompanied by a notable rise in the accumulation of a major protein band with a molecular weight of 39 kDa, consistent with the molecular weight of previously characterized <em>oph</em> proteins. Of particular interest, the toxicity of CP degradation products resulting from both CP-bacterial biodegradation experiments exhibited minimal effects on neural cells, as indicated by acetylcholinesterase (AChE) activity and cell viability, underscoring the safety of CP-metabilties. Our study underscores the exceptional biodegradation capability of <em>P. aryabhattai</em> 1–3I in breaking down CP in soil, highlighting its potential for effective remediation of CP-contaminated environments.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106416"},"PeriodicalIF":4.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological roles of trehalose in Hyphantria cunea revealed by RNA interference of trehalose-6-phosphate synthase and trehalase genes","authors":"Hongqu Wu ,&nbsp;Chunmiao Sun ,&nbsp;Yuanwang Wu ,&nbsp;Chuanwang Cao ,&nbsp;Lili Sun","doi":"10.1016/j.pestbp.2025.106422","DOIUrl":"10.1016/j.pestbp.2025.106422","url":null,"abstract":"<div><div>Trehalose plays a crucial role in insect energy metabolism and stress tolerance. Therefore, we investigated the physiological functions of trehalose metabolism genes, namely trehalose-6-phosphate synthase (<em>TPS</em>), soluble trehalase (<em>Tre1</em>), and membrane-bound trehalase (<em>Tre2</em>) were investigated in <em>Hyphantria cunea</em> using RNA interference. Silencing of <em>TPS</em>, <em>Tre1</em>, and <em>Tre2</em> genes achieved silencing efficiencies of 51.77, 71.38 and 52.01 %, respectively, at 72 h post-injection. Silencing <em>TPS</em> increased food intake, body weight, prolonged development duration, and decreased glucose, trehalose, and glycogen levels, and pupal weight. In contrast, silencing <em>Tre1</em> and <em>Tre2</em> decreased food intake, body weight, glucose and glycogen levels, and pupal weight, delayed development, and increased trehalose content. Silencing <em>TPS</em>, <em>Tre1</em>, and <em>Tre2</em> caused abnormal phenotypes, such as pupal and wing deformities. Silencing <em>TPS</em> suppressed the expression of five genes in the chitin biosynthesis pathway and two to lipid catabolism related genes. The expression levels of two genes associated with lipid biosynthesis were upregulated, and as <em>Tre1</em> and <em>Tre2</em> were significantly downregulated after <em>TPS</em> RNAi, while <em>UAP</em> and <em>CHSA</em> expression levels were specifically affected by RNAi of <em>TPS</em> and <em>Tre1</em>. In female <em>H. cunea</em> adults, silencing <em>TPS</em>, <em>Tre1</em>, and <em>Tre2</em> genes significantly reduced the number of eggs conceived, and laid and egg hatchability. Overall, silencing <em>TPS</em>, <em>Tre1</em>, and <em>Tre2</em> genes disrupted trehalose metabolism, affecting the growth, development, and reproduction of <em>H. cunea</em>. These findings highlight the potential for targeting trehalose metabolism genes as an environmentally friendly pest management strategy.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106422"},"PeriodicalIF":4.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Odorant receptor 75 is essential for attractive response to plant volatile p-anisaldehyde in Western flower thrips","authors":"Xuan-Pu Luan ,&nbsp;Xiao-Tong Zhang ,&nbsp;Zhi-Qiang Wei ,&nbsp;Jin-Meng Guo ,&nbsp;George F.O. Obiero ,&nbsp;Merid N. Getahun ,&nbsp;Qi Yan ,&nbsp;Jin Zhang ,&nbsp;Shuang-Lin Dong","doi":"10.1016/j.pestbp.2025.106421","DOIUrl":"10.1016/j.pestbp.2025.106421","url":null,"abstract":"<div><div>The Western flower thrip (WFT), <em>Frankliniella occidentalis,</em> is a major pest of many vegetable crops and also a vector for the tomato spotted wilt virus, causing devastating damage worldwide. Odorant receptors (ORs) play an important role in host plant searching, however, specific functions of those ORs in WFT remain unclear. In this study, the attractive activity of four plant volatiles ((<em>S</em>)-(−)-verbenone, <em>p-</em>anisaldehyde, methyl isonicotinate, and benzaldehyde) to WFT was confirmed using a Y-tube olfactometer. Then, the specific receptor, OR75, was screened out as the candidate OR for these odorants, as its expression was significantly upregulated upon exposure to these odorants. Further <em>in vitro</em> functional assays with <em>Xenopus</em> oocyte expression system confirmed sensitivity of OR75 to <em>p-</em>anisaldehyde and three other odorants (<em>β-</em>ionone, undecanal and cinnamaldehyde). Of the three odorants, <em>β-</em>ionone was also attractive to WFT. Further, <em>in vivo</em> RNA interference experiments showed that the dsOR75 treated thrips lost their attractive response to <em>p-</em>anisaldehyde, but retained response to <em>β-</em>ionone. Finally, 3-D structures prediction and molecular docking showed that OR75 formed a hydrogen bond with <em>p-</em>anisaldehyde at His150 residue, while no hydrogen bond formed with <em>β-</em>ionone, undecanal or cinnamaldehyde. Taken together, OR75 plays a crucial role in perception of <em>p-</em>anisaldehyde, which helps us understand the host-seeking mechanisms of WFT, and provides a basis for development of olfactory based pest control strategies. This is the first report of an OR playing roles in sensing <em>p-</em>anisaldehyde in thrips.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106421"},"PeriodicalIF":4.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on enzymatic colorimetric assays for organophosphate and carbamate pesticides detection in water environments","authors":"Eliasu Issaka,&nbsp;Lynsey Melville,&nbsp;Adnan Fazal","doi":"10.1016/j.pestbp.2025.106423","DOIUrl":"10.1016/j.pestbp.2025.106423","url":null,"abstract":"<div><div>To monitor pesticides, which have grown to be a significant environmental and public health concern, sensitive, selective, and economical analytical tools must be developed. With advantages including high sensitivity, quick processing, and the potential for on-site monitoring, enzymatic colourimetric assays have surfaced as a potential substitute for conventional pesticide detection, particularly for organophosphate (OPPs) and carbamate pesticide detection. The toxicological effects of pesticides on humans and the environment are examined first in this review, followed by examining the concepts and mechanisms behind enzyme activity and colourimetric methods. Besides, single and double-enzyme-mediated colourimetric techniques are also studied to detect OPPs and carbamate pesticides. Furthermore, colourimetric smartphone platforms and paper-based devices have both garnered a lot of attention. These advanced approaches offer many pesticide detection options, from high-sensitivity lab-based procedures to on-site and in-field technologies. The fourth section of this review employs newly published studies to explore the applicability of these approaches for onsite OPPs and carbamate pesticide detection. Lastly, the challenges associated with enzymatic colourimetric assays, such as matrix effects and enzyme stability, and prospects for current and future research are discussed.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106423"},"PeriodicalIF":4.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ROS acted as an initial role in selenium nanoparticles alleviating insecticide chlorpyrifos-induced oxidative stress, pyroptosis, and intestinal barrier dysfunction in porcine intestinal epithelial cells","authors":"Minna Qiu ,&nbsp;Zhiyu Hao ,&nbsp;Yuhao Liu ,&nbsp;Yuhang Liu ,&nbsp;Minghang Chang ,&nbsp;Xu Lin ,&nbsp;Xiumei Liu ,&nbsp;Na Dong ,&nbsp;Wei Sun ,&nbsp;Xiaohua Teng","doi":"10.1016/j.pestbp.2025.106418","DOIUrl":"10.1016/j.pestbp.2025.106418","url":null,"abstract":"<div><div>Chlorpyrifos (CPF), a toxic organophosphorus insecticide, is widely used in agriculture to protect crops (eg., maize) from pests. The use of CPF in crops can result in accumulation in crop seeds, such as corn seeds, which is a primary feed ingredient in pigs. Pigs in China, which is an important source of animal-derived protein in the Chinese diet, account for over 50 % of the raised pig population in the whole world. Therefore, CPF may pose a potential risk to the health of non-target organisms (pigs and humans) through the food chain. However, whether CPF can damage porcine intestine remains unknown. Selenium (Se), an essential trace element, was reported to have antioxidant and anti-toxic effects. Tight junction (TJ) is an important mechanism of intestinal injury and pyroptosis is a new hotspot in the field of toxicology. Hence, we wanted to investigate whether CPF can damage pig intestine and whether selenium nanoparticles (SeNPs) supplement can alleviate CPF-induced pig intestine damage, and to study corresponding mechanism from the three aspects of OS, pytoptosis, and TJ. We established a model of SeNPs alleviating damage caused by CPF in intestinal porcine enterocytes (IPEC-J2 cells), and found that SeNPs alleviated CPF-induced oxidative stress (OS), pyroptosis, and intestinal barrier dysfunction in IPEC-J2 cells. Interestingly, OS, pyroptosis, and intestinal barrier dysfunction had serial relations, and ROS/Nrf2/Caspase-1/Occludin and ROS/Nrf2/Caspase-1/ZO-1 pathways played a role. Notably, ROS and Caspase-1 played an initial and important role, respectively. Our study added new information on pesticides-caused damage to non-target organisms, and provided new idea, insight, and targets to mitigatie pesticides-induced toxic effect on non-target organisms.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106418"},"PeriodicalIF":4.2,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNAi-mediated knockdown of juvenile hormone acid methyltransferase depresses reproductive performance in female Aethina tumida","authors":"Yifan Gu ,&nbsp;Xinyu Yang ,&nbsp;Senhao Liu ,&nbsp;Xiaowei Chen ,&nbsp;Ran Liu ,&nbsp;Jinglin Gao ,&nbsp;Yihai Zhong ,&nbsp;Xiaoyu Li ,&nbsp;Wensu Han","doi":"10.1016/j.pestbp.2025.106420","DOIUrl":"10.1016/j.pestbp.2025.106420","url":null,"abstract":"<div><div>Small hive beetles, <em>Aethina tumide</em>, are free-flying parasites of social bee colonies where they feed and reproduce. In case of mass infestation, <em>A. tumida</em> can cause significant economic losses. There is an urgent need to explore novel green molecular approaches for sustainable control of <em>A. tumida</em>. It has been confirmed that juvenile hormone acid methyl transferase (<em>JHAMT</em>) plays a crucial role in regulating the synthesis of juvenile hormone (JH). However, its impact on female reproduction of <em>A. tumida</em> remains unclear. In the present study, a novel <em>JHAMT</em> gene was identified from <em>A. tumida</em> with an open reading frame of 978 bp, encoding a polypeptide of 325 amino acids containing a Methyltransferase domain. The deduced amino acid sequence of AtJHAMT shared 60 % and 33 % identity with homologs from <em>Brassicogethes aeneus</em> and <em>Apis mellifera</em>, respectively. The expression profile indicates that the transcription level of <em>AtJHAMT</em> increases in the adult stages, reaching its peak in 5-day-old female adults. <em>AtJHAMT</em> exhibits the highest expression levels in the ovaries, and fluorescence in situ hybridization (FISH) demonstrates that this gene shows a significant number of positive signals in the ovarian ducts and the head region. Furthermore, we investigated the function of <em>AtJHAMT</em> through RNA interference and methoprene rescue experiments. We also investigeted the off-target effects of the <em>dsJHAMT</em>. The results showed that silencing <em>AtJHAMT</em> through oral dsRNA delivery (feeding dsRNA-SPc mix) affected ovarian development and significantly reduced JH titers, female fecundity, female fertility, and egg hatchability. The application of methoprene partially rescued the negative effect of silencing <em>AtJHAMT</em> on reproduction. Several genes associated with ovarian development were significantly downregulated following interference with <em>AtJHAMT</em>, but their expression levels were restored after complementation experiments. Additionally, the off-target effects experiment showed that <em>dsJHAMT</em> from <em>A. tumida</em> had no adverse effects on ovaries development in honey bee queens. Overall, this study illustrates the functions of the <em>JHAMT</em> in <em>A. tumida</em>, which can serve as a potential target for controlling the reproduction of the most deleterious bee parasites, <em>A. tumida</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106420"},"PeriodicalIF":4.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid surface droplet 2 (LSD2) regulates lipid metabolism and male reproductive physiology in adult Sitotroga cerealella","authors":"Sakhawat Shah ,&nbsp;Yu Li ,&nbsp;Wen-Han Yan ,&nbsp;Lianyun Liu ,&nbsp;Tianyu Gu ,&nbsp;Karam Khamis Elgizawy ,&nbsp;Gang Wu ,&nbsp;Feng-Lian Yang","doi":"10.1016/j.pestbp.2025.106417","DOIUrl":"10.1016/j.pestbp.2025.106417","url":null,"abstract":"<div><div>Insect metabolism plays a critical role in shaping reproductive physiology, offering valuable insight for pest management strategies and ecological understanding. In this study, we investigated the regulation of the lipid surface droplet protein <em>LSD2</em> and its impact on lipid metabolism and male reproductive events in <em>Sitotroga cerealella</em>. Using <em>ScLSD2</em> RNAi, we assessed it effects on energy metabolism, sperm count, motility, oviposition, and hatchability in <em>S. cerealella</em> adults. Our results reveal tissue-specific expression of <em>ScLSD2</em>, predominantly in the fat body. Silencing <em>ScLSD2</em> led to reduced mating rates, sperm motility parameters, oviposition, and hatchability, alongside alteration in energy metabolites, including decreased in lipid droplets, triglyceride and glycerol levels and increased diglyceride content. Additionally, sperm quantification revealed significant reductions in eupyrene sperm count following <em>ScLSD2</em> silencing, indicating impaired reproductive function. These results show the pivotal role of LSD2 in linking lipid metabolism and reproductive physiology in <em>S. cerealella</em>, positioning it as a promising target for pest management strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106417"},"PeriodicalIF":4.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silencing the β-glucan recognition protein enhanced the pathogenicity of Cordyceps fumosorose against Hyphantria cunea Drury larvae","authors":"Wenxuan Li ,&nbsp;Jianyang Bai ,&nbsp;Jundan Deng ,&nbsp;Weikang Xu ,&nbsp;Qing-He Zhang ,&nbsp;Jacob D. Wickham ,&nbsp;Mengting Wu ,&nbsp;Longwa Zhang","doi":"10.1016/j.pestbp.2025.106415","DOIUrl":"10.1016/j.pestbp.2025.106415","url":null,"abstract":"<div><div>The β-glucan recognition protein (<em>βGRP</em>) plays a crucial role in pathogen recognition by insects, thereby activating their innate immunity. However, the immune response of <em>βGRP</em> in <em>Hyphantria cunea</em> Drury (Lepidoptera: Noctuidae), an invasive pest of forests and agriculture, to pathogens remains unclear. In this study, we identified a new isolate of the entomopathogenic fungus <em>Cordyceps fumosorosea</em>. We found <em>C. fumosorosea</em> exhibits significant pathogenicity against <em>H. cunea</em> larvae. Based on the transcriptome, we found that the <em>βGRP</em> genes of <em>H. cunea</em> can be induced to express after infection by <em>C. fumosorosea</em>. <em>βGRP1</em> is primarily expressed in the fat body and significantly upregulated by 11.23-fold at 12 h post-infection with <em>C. fumosorosea</em>. Besides, molecular docking showed a potential binding interaction between <em>β</em>GRP1 protein and β-1,3-glucans, which is further confirmed by protein-carbohydrate binding assays. Additionally, the knockdown of <em>βGRP1</em> through RNA interference increases the mortality of <em>H. cunea</em> larvae following <em>C. fumosorosea</em> infection. Taken together, our study underscores the critical role of <em>βGRP1</em> in the immune response to <em>C. fumosorosea</em> infection and suggests an integrated pest management strategy that combines entomopathogenic fungi with RNA interference technology as an effective approach for controlling <em>H. cunea</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106415"},"PeriodicalIF":4.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NADPH-cytochrome P450 reductase mediates resistance to neonicotinoid insecticides in Bradysia odoriphaga","authors":"Xinxiang Wang,&nbsp;Xianglong Chen,&nbsp;Taoling Zhou,&nbsp;Wu Dai,&nbsp;Chunni Zhang","doi":"10.1016/j.pestbp.2025.106406","DOIUrl":"10.1016/j.pestbp.2025.106406","url":null,"abstract":"<div><div>As a crucial electron transfer partner of the P450 system, NADPH-cytochrome P450 reductase (CPR) plays an influential role in P450-mediated detoxification metabolism of xenobiotics. CPR has been found to be associated with insecticide resistance in several insects. However, the role of CPR in the cross-resistance of <em>Bradysia odoriphaga</em> to clothianidin and neonicotinoid insecticides remains to be elucidated. In this study, the <em>CPR</em> gene (<em>BoCPR</em>) of <em>B. odoriphaga</em> was cloned and characterized. The expression of <em>BoCPR</em> was more abundant in the adult stage and in the midgut and Malpighian tubules of larvae, and <em>BoCPR</em> was significantly overexpressed in the clothianidin-resistant (CL-R) strain compared to the susceptible (SS) strain. Exposure to clothianidin significantly increased <em>BoCPR</em> expression in both the SS and CL-R strains. In addition, knockdown of <em>BoCPR</em> in SS and CL-R strains significantly reduced CPR and P450 enzyme activities, and resulted in a significant increase in larval susceptibility to clothianidin, imidacloprid, and thiamethoxam. These results suggest that <em>BoCPR</em> plays an important role in <em>B. odoriphaga</em> resistance to clothianidin and cross-resistance to neonicotinoid insecticides.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106406"},"PeriodicalIF":4.2,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}