Chunmei You , Ruiguan Duan , Yijuan Chen , Ying Liu , Xiangyun Ji
{"title":"夜蛾对氯虫腈和甲维菌素苯甲酸酯耐受相关ABCB转运基因的鉴定与验证","authors":"Chunmei You , Ruiguan Duan , Yijuan Chen , Ying Liu , Xiangyun Ji","doi":"10.1016/j.pestbp.2025.106686","DOIUrl":null,"url":null,"abstract":"<div><div><em>Spodoptera exigua</em> is a significant invasive pest that has developed resistance to various commonly applied insecticides. The detoxification of insecticides in insects may partially depend on ATP-binding cassette (ABC) transporters. Although the functions of <em>ABCB</em> subfamily genes are diverse in insects, the specific roles of <em>ABCBs</em> in <em>S. exigua</em> remain largely unexplored. This research focused on identifying ten <em>SeABCB</em> genes and characterizing their expression profiles across various developmental stages and tissues, as well as in response to different insecticide treatments. Our findings indicate that <em>SeABCB</em> genes are expressed at all developmental stages, with most ABC transporters exhibiting higher expression levels during the pupal and adult stages compared to the larval stage. Furthermore, high expression levels of <em>SeABCBs</em> were observed in diverse tissues: <em>SeABCB1</em> and <em>SeABCB6</em> were found to be more abundant in the midgut, while <em>SeABCB3</em> and <em>SeABCB4</em> exhibited significantly higher expression in fat bodies. The expression of <em>SeABCB2</em>, <em>SeABCB5</em> and <em>SeABCB8</em> was notable in Malpighian tubules, and <em>SeABCB9</em> showed a higher expression level in the cuticle than in other tissues. Eight <em>ABCBs</em> were upregulated in <em>S. exigua</em> following exposure to chlorantraniliprole and emamectin benzoate. Gene functional analysis based on RNAi proved that <em>SeABCB4</em> and <em>SeABCB9</em> are involved in the tolerance of <em>S. exigua to</em> chlorantraniliprole, while <em>SeABCB1</em> and <em>SeABCB9</em> participate in the transport or metabolism of emamectin benzoate in <em>S. exigua</em>. In summary, our research provides significant insights into the role of <em>SeABCBs</em> in the detoxification mechanisms of <em>S. exigua</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106686"},"PeriodicalIF":4.0000,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification and validation of ABCB transporter genes involved in chlorantraniliprole and emamectin benzoate tolerance in Spodoptera exigua\",\"authors\":\"Chunmei You , Ruiguan Duan , Yijuan Chen , Ying Liu , Xiangyun Ji\",\"doi\":\"10.1016/j.pestbp.2025.106686\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Spodoptera exigua</em> is a significant invasive pest that has developed resistance to various commonly applied insecticides. The detoxification of insecticides in insects may partially depend on ATP-binding cassette (ABC) transporters. Although the functions of <em>ABCB</em> subfamily genes are diverse in insects, the specific roles of <em>ABCBs</em> in <em>S. exigua</em> remain largely unexplored. This research focused on identifying ten <em>SeABCB</em> genes and characterizing their expression profiles across various developmental stages and tissues, as well as in response to different insecticide treatments. Our findings indicate that <em>SeABCB</em> genes are expressed at all developmental stages, with most ABC transporters exhibiting higher expression levels during the pupal and adult stages compared to the larval stage. Furthermore, high expression levels of <em>SeABCBs</em> were observed in diverse tissues: <em>SeABCB1</em> and <em>SeABCB6</em> were found to be more abundant in the midgut, while <em>SeABCB3</em> and <em>SeABCB4</em> exhibited significantly higher expression in fat bodies. The expression of <em>SeABCB2</em>, <em>SeABCB5</em> and <em>SeABCB8</em> was notable in Malpighian tubules, and <em>SeABCB9</em> showed a higher expression level in the cuticle than in other tissues. Eight <em>ABCBs</em> were upregulated in <em>S. exigua</em> following exposure to chlorantraniliprole and emamectin benzoate. Gene functional analysis based on RNAi proved that <em>SeABCB4</em> and <em>SeABCB9</em> are involved in the tolerance of <em>S. exigua to</em> chlorantraniliprole, while <em>SeABCB1</em> and <em>SeABCB9</em> participate in the transport or metabolism of emamectin benzoate in <em>S. exigua</em>. In summary, our research provides significant insights into the role of <em>SeABCBs</em> in the detoxification mechanisms of <em>S. exigua</em>.</div></div>\",\"PeriodicalId\":19828,\"journal\":{\"name\":\"Pesticide Biochemistry and Physiology\",\"volume\":\"215 \",\"pages\":\"Article 106686\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2025-09-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pesticide Biochemistry and Physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0048357525003992\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticide Biochemistry and Physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0048357525003992","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Identification and validation of ABCB transporter genes involved in chlorantraniliprole and emamectin benzoate tolerance in Spodoptera exigua
Spodoptera exigua is a significant invasive pest that has developed resistance to various commonly applied insecticides. The detoxification of insecticides in insects may partially depend on ATP-binding cassette (ABC) transporters. Although the functions of ABCB subfamily genes are diverse in insects, the specific roles of ABCBs in S. exigua remain largely unexplored. This research focused on identifying ten SeABCB genes and characterizing their expression profiles across various developmental stages and tissues, as well as in response to different insecticide treatments. Our findings indicate that SeABCB genes are expressed at all developmental stages, with most ABC transporters exhibiting higher expression levels during the pupal and adult stages compared to the larval stage. Furthermore, high expression levels of SeABCBs were observed in diverse tissues: SeABCB1 and SeABCB6 were found to be more abundant in the midgut, while SeABCB3 and SeABCB4 exhibited significantly higher expression in fat bodies. The expression of SeABCB2, SeABCB5 and SeABCB8 was notable in Malpighian tubules, and SeABCB9 showed a higher expression level in the cuticle than in other tissues. Eight ABCBs were upregulated in S. exigua following exposure to chlorantraniliprole and emamectin benzoate. Gene functional analysis based on RNAi proved that SeABCB4 and SeABCB9 are involved in the tolerance of S. exigua to chlorantraniliprole, while SeABCB1 and SeABCB9 participate in the transport or metabolism of emamectin benzoate in S. exigua. In summary, our research provides significant insights into the role of SeABCBs in the detoxification mechanisms of S. exigua.
期刊介绍:
Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance.
Research Areas Emphasized Include the Biochemistry and Physiology of:
• Comparative toxicity
• Mode of action
• Pathophysiology
• Plant growth regulators
• Resistance
• Other effects of pesticides on both parasites and hosts.