Pesticide Biochemistry and Physiology最新文献

Insecticide-induced sublethal effect in the fall armyworm is mediated by miR-9993/miR-2a-3p - FPPS/JHAMT - JH molecular module

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-04-01 DOI: 10.1016/j.pestbp.2025.106400

Aiyu Wang , Yuanxue Yang , Yun Zhang , Chao Xue , Yinjie Cheng , Yifei Zhang , Wenjuan Zhang , Ming Zhao , Jianhua Zhang

{"title":"Insecticide-induced sublethal effect in the fall armyworm is mediated by miR-9993/miR-2a-3p - FPPS/JHAMT - JH molecular module","authors":"Aiyu Wang , Yuanxue Yang , Yun Zhang , Chao Xue , Yinjie Cheng , Yifei Zhang , Wenjuan Zhang , Ming Zhao , Jianhua Zhang","doi":"10.1016/j.pestbp.2025.106400","DOIUrl":"10.1016/j.pestbp.2025.106400","url":null,"abstract":"<div><div>The sublethal effect of insecticides can affect the population dynamics of pests by changing the physiological or behavioral changes, which poses a serious threat to the sustainable control of crop pests in the field. However, the molecular regulation mechanism that mediates the sublethal effect of insecticides on crop pests remains unsolved. Here, we show that the sublethal effect of spinetoram and cyantraniliprole on <em>Spodoptera frugiperda</em> is mediated by the molecular module of microRNA-9993/microRNA-2a-3p - farnesyl diphosphate synthase gene (<em>FPPS</em>)/juvenile hormone (JH) acid methyltransferase gene (<em>JHAMT</em>) - JH. Spinetoram prolonged the duration of larvae and pupae, decreased the weight of pupae, while cyantraniliprole prolonged the duration of larvae and decreased the emergence rate. Similarly, injection of the juvenile hormone analogue (JHA) methoprene significantly prolonged the developmental duration of larvae and pupae, decreased the pupal weight and emergence rate. This sublethal phenotypic change was due to the upregulation of key JH synthesis genes, including <em>FPPS</em> and <em>JHAMT</em>, mediated by spinetoram and cyantraniliprole, resulting in an increase in JH titer. Furthermore, it was confirmed by small RNA sequencing, dual luciferase analysis and agomir-miRNA injection, miR-9993 and miR-2a-3p that it could bind <em>FPPS</em> and <em>JHAMT</em> respectively, and regulated the expression level of <em>FPPS</em> and <em>JHAMT</em> to affect the titer of JH, thus changing the phenotype of <em>S. frugiperda</em>. Collectively, these results provide insights into the mechanism of insecticide regulation of sublethal effects of pests, expand our understanding of development-related miRNAs, and reveal key factors involved in JH signaling pathways that support sublethal effects of insecticides.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106400"},"PeriodicalIF":4.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison on the conformation folding and structure change of serum albumin induced by methyl parathion and its metabolite p-nitrophenol

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-30 DOI: 10.1016/j.pestbp.2025.106393

Lu Chen, Zhu Wang, Xianglong Wu, Qiulan Zhang, Yongnian Ni

{"title":"Comparison on the conformation folding and structure change of serum albumin induced by methyl parathion and its metabolite p-nitrophenol","authors":"Lu Chen, Zhu Wang, Xianglong Wu, Qiulan Zhang, Yongnian Ni","doi":"10.1016/j.pestbp.2025.106393","DOIUrl":"10.1016/j.pestbp.2025.106393","url":null,"abstract":"<div><div>Residues of organophosphorus pesticides (OPPs) and their metabolites pose potential risks to the environment and human health. In the work, multiple spectroscopy, atomic force microscope and computational simulations were utilized to compare the interaction between methyl parathion (MP) and its metabolite p-nitrophenol (PNP) with human serum albumin (HSA). The results showed that both MP and PNP spontaneously formed complexes with HSA predominantly facilitated by hydrogen bonds and van der Waals forces, following static quenching mechanisms. The binding constant of PNP (15.16 ± 0.10 × 10<sup>4</sup> L mol<sup>−1</sup>) with HSA was nearly 5 times larger than that of MP (3.58 ± 0.09 × 10<sup>4</sup> L mol<sup>−1</sup>), suggesting PNP had a stronger affinity with HSA, which was consistent with density functional theory (DFT) calculation. Molecular docking revealed that the binding energy of PNP (−4.54 kcal mol<sup>−1</sup>) was lower than that of MP (−4.07 kcal mol<sup>−1</sup>), which potentially contributed a longer in vivo half-life of PNP and greater potential harm. Moreover, synchronous, 3D, FTIR and CD spectroscopy analyses indicated that the binding of MP and PNP to HSA significantly altered the microenvironment of amino acid residues and the secondary structure of HSA. Molecular dynamics simulations further demonstrated these findings. The study provides insights on the interaction between the pesticide MP and its metabolite PNP with HSA, which help understand the impact of pesticide residues on the food safety and environmental protection at the molecular level.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106393"},"PeriodicalIF":4.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spirodiclofen inhibited melanin synthesis in zebrafish embryos

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-29 DOI: 10.1016/j.pestbp.2025.106397

LiangHao Suo, FuYu Hou, ZiYu Wang, ChunHui Wu, Jia Xie, WeiGuo Miao, YongMei Fan, Jie Zhang

{"title":"Spirodiclofen inhibited melanin synthesis in zebrafish embryos","authors":"LiangHao Suo, FuYu Hou, ZiYu Wang, ChunHui Wu, Jia Xie, WeiGuo Miao, YongMei Fan, Jie Zhang","doi":"10.1016/j.pestbp.2025.106397","DOIUrl":"10.1016/j.pestbp.2025.106397","url":null,"abstract":"<div><div>Spirodiclofen has been registered and marketed in more than 50 countries worldwide and are widely used because of their broad-spectrum acaricidal activity and long-lasting efficacy. However, its environmental toxicological assessment to fish remains poorly understood. In the present study, zebrafish embryos were modelled and exposed to series concentration of spirodiclofen. It has been found that spirodiclofen exposure induced zebrafish embryos abnormal pigmentation, the quantitative analysis of melanin in images using Image J showed a significant decrease in the proportion of melanin area in zebrafish exposed to 0.146 mg/L treatment group at 48 and 96 h, respectively. ELISA analysis illustrated that zebrafish embryos exposed to 0.146 mg/L exhibited a significant decrease in the levels of melanin, tyrosinase and dopachrome tautomerase content, and in constant with these results, the genes involved in melanin synthesis (<em>Tyr</em>, <em>Dct</em> and <em>Pck-β</em>) were significantly downregulated, indicating that melanin synthesis was inhibited. The molecular docking showed that spirodiclofen had a lower binding energy with tyrosinase compared to other compounds. The results demonstrated that spirodiclofen interfered zebrafish embryos melanin synthesis. This provided new insights into the mechanism of spirodiclofen toxicity to zebrafish embryos.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106397"},"PeriodicalIF":4.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polystyrene-microplastics and Emamectin Benzoate co-exposure induced lipid remodeling by suppressing PPARα signals to drive ACSL4-dependent ferroptosis and carp splenic injury

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-29 DOI: 10.1016/j.pestbp.2025.106396

Hao Wu , Tong Xu , Naixi Yang , Shiwen Xu

{"title":"Polystyrene-microplastics and Emamectin Benzoate co-exposure induced lipid remodeling by suppressing PPARα signals to drive ACSL4-dependent ferroptosis and carp splenic injury","authors":"Hao Wu , Tong Xu , Naixi Yang , Shiwen Xu","doi":"10.1016/j.pestbp.2025.106396","DOIUrl":"10.1016/j.pestbp.2025.106396","url":null,"abstract":"<div><div>Microplastics (MPs) and Emamectin Benzoate (EMB) were identified as hazardous environmental pollutants, frequently coexisting in aquatic ecosystems, posing potential risk in the immune system of human and animal. However, the hazards of concurrent exposed to MPs and EMB on the carp spleen, and the specific mechanisms remain unclear. Here, we employed MPs and EMB-exposed carp models, and cultured splenocytes in vitro, to demonstrate that PPARα signals suppression underlay MPs and EMB-induced carp spleen injury, based on transcriptomics and lipomics analysis. This suppression exacerbated the buildup of polyunsaturated fatty acid (PUFA), and promoted ACSL4 expression, resulting in increased lipid peroxidation. Further studies found that the accumulation of lipid peroxides predominantly occurred in the mitochondria, which evoked mitochondrial homeostasis imbalance and compromised mitochondrial function, thereby initiating ferroptosis. Additionally, enhancing PPARα signaling, inhibiting ACSL4, or scavenging mitochondrial ROS was favor of mitigating accumulation of lipid peroxides, reducing mitochondrial damage and inhibiting ferroptosis. Notably, MPs and EMB co-exposure caused more severe damage than single exposure. These findings uncovered a potential mechanism, involving PPARα signaling inhibition by MPs and EMB co-exposure, which evoked lipid remodeling and increased ACSL4, to drive ferroptosis and carp splenic injury. This study highlighted the potential hazards to the aquaculture environments where co-exposure of MPs and EMB and provided reference for environmental toxicology research and the sustainable development of the aquaculture industry.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106396"},"PeriodicalIF":4.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organic amendments restore soil biological properties under pesticides application

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-25 DOI: 10.1016/j.pestbp.2025.106394

Ahmad Latif Virk , Awais Shakoor , Naeem Ahmad , Huaqiang Du , Scott X. Chang , Yanjiang Cai

{"title":"Organic amendments restore soil biological properties under pesticides application","authors":"Ahmad Latif Virk , Awais Shakoor , Naeem Ahmad , Huaqiang Du , Scott X. Chang , Yanjiang Cai","doi":"10.1016/j.pestbp.2025.106394","DOIUrl":"10.1016/j.pestbp.2025.106394","url":null,"abstract":"<div><div>Pesticides are indispensable in modern agriculture, but they also affect soil biological properties and soil sustainability. Although organic amendments (OAs) have the potential to alleviate the negative effects of pesticides and restore soil biological properties, but results have been inconsistent. Here, we conducted a meta-analysis to study the overall effects of OAs on soil biological properties under pesticide application. Our results showed that sole pesticide application significantly decreased soil enzymatic activities (i.e., by −2.5 % for urease, −22 % for β-glucosidase, and − 10 % for dehydrogenase) and reduced microbial population size (e.g., by −6 % for microbial biomass, −10 % for actinomycetes population, −7 % for bacteria and − 12 % for fungi population). Noticeably, sole fungicide application did not significantly affect soil bacteria, fungi and actinomycetes populations as compared to sole herbicide and insecticide. In contrast, pesticide with OAs application significantly increased soil respiration (+60 %), activities of dehydrogenase (+73 %), urease (+80 %) and β-glucosidase (+65 %), microbial biomass (+79 %), bacterial (+65 %) and fungal (+13 %) population sizes than sole pesticide. However, soil acid phosphatase activity significantly decreased (−17 %) by using pesticide with OAs. Our results suggest that OAs in combination with pesticides can modify soil physicochemical properties and subsequently restore soil biological properties, but the magnitude of improvement of specific OAs in combination with individual pesticide classes remains unknown and should be studied in future research.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106394"},"PeriodicalIF":4.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid spread of Amitraz resistance linked to a unique T115N mutation in the octopamine receptor of Varroa mites in Korea

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106387

Joonhee Lee , Jong Hyeok Lee , Youngcheon Lim , Susie Cho , KyungHwan Moon , Sanghyeon Kim , Young Ho Kim , Si Hyeock Lee

{"title":"Rapid spread of Amitraz resistance linked to a unique T115N mutation in the octopamine receptor of Varroa mites in Korea","authors":"Joonhee Lee , Jong Hyeok Lee , Youngcheon Lim , Susie Cho , KyungHwan Moon , Sanghyeon Kim , Young Ho Kim , Si Hyeock Lee","doi":"10.1016/j.pestbp.2025.106387","DOIUrl":"10.1016/j.pestbp.2025.106387","url":null,"abstract":"<div><div>The <em>Varroa</em> mite, <em>Varroa destructor</em>, is an ectoparasitic pest of Western honey bees and poses a significant threat to apiculture. In Korea, widespread fluvalinate resistance has increased reliance on amitraz as an alternative acaricide. This study identified a novel mutation, T115N, in the β2-adrenergic-like octopamine receptor gene (<em>Octβ</em><sub><em>2</em></sub><em>R</em>) and confirmed its role in amitraz resistance. Genotyping of individual mites, based on time-dependent intoxication responses to a diagnostic dose of amitraz, revealed a strong correlation between genotype and resistance phenotype: mites with early intoxication responses were homozygous for the T115 allele, whereas those with delayed responses predominantly carried the homozygous N115 allele. Mites with homozygous N115 genotype exhibited a median lethal time approximately 2.8-fold longer than their T115 counterparts, confirming the resistance conferred by the T115N mutation. Quantitative sequencing was established to track the spread dynamics of T115N mutation in Korean <em>Varroa</em> mite populations over five years (2020–2024). While the mutation was rare from 2020 to 2022, its frequency increased sharply in 2023 and became widespread across Korea by 2024, coinciding with increased amitraz usage. These findings suggest region-specific selection for the T115N mutation driven by amitraz exposure.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106387"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The involvement of thioredoxin reductase genes in development, reproduction and deltamethrin tolerance in the red flour beetle, Tribolium castaneum

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106390

Caihong Ji , Daojie Guan , Haoting Chen , Zhichao Luo , Chengyun Jian , Zhichao Wang , Huichen Ge , Kun Qian , Jianjun Wang

{"title":"The involvement of thioredoxin reductase genes in development, reproduction and deltamethrin tolerance in the red flour beetle, Tribolium castaneum","authors":"Caihong Ji , Daojie Guan , Haoting Chen , Zhichao Luo , Chengyun Jian , Zhichao Wang , Huichen Ge , Kun Qian , Jianjun Wang","doi":"10.1016/j.pestbp.2025.106390","DOIUrl":"10.1016/j.pestbp.2025.106390","url":null,"abstract":"<div><div>As an essential component of the thioredoxin system, thioredoxin reductase (TrxR) plays an important role in maintaining redox homeostasis in mammalian cells, however, functional characterization of insect TrxRs is still limited. In this study, full-length cDNAs of <em>TcTrxR1</em> and <em>TcTrxR2</em> were cloned from the red flour beetle, <em>Tribolium castaneum.</em> Sequence analysis revealed the highly conserved active site motifs CVNVGC and CCS at the N-terminal and C-terminal of TcTrxR1, respectively, whereas TcTrxR2 lacks these two conserved motifs. Analysis of the spatio-temporal expression pattern by RT-qPCR showed that the expression of <em>TcTrxR1</em> was the highest in 1-day-old larva and brain, and <em>TcTrxR2</em> was highly expressed in eggs and fat body, respectively. Further functional analysis by RNA interference (RNAi) revealed that knockdown of <em>TcTrxR1</em> and <em>TcTrxR2</em> at the larval stage led to 100 % and 98.67 % mortality of larvae beetles, and pupal RNAi of <em>TcTrxR1</em> and <em>TcTrxR2</em> resulted in decreased eclosion rates as well as failure of the female adults to lay eggs. Additionally, injection of dsTcTrxR2 decreased the tolerance of beetles to deltamethrin, whereas knockdown of <em>TcTrxR1</em> significantly increased the tolerance of beetles to deltamethrin. Notably, knockdown of <em>TcTrxR1</em> significantly upregulated the expression of <em>TcCYP6BQ2</em>, <em>TcCYP6BQ4</em> and <em>TcCYP6BQ7</em>, and led to nuclear translocation of transcription factor CncC, a major regulator of detoxification in insects. These findings provide insights into the function of insect <em>TrxRs</em> as well as the regulatory mechanisms of CncC, and have applied implications for the RNAi-based insect pest control.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106390"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional analysis of Ophraella communa Lesage OcomOBP11 in recognition of Ambrosia artemisiifolia L. volatiles

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106392

Yang Yue , Chao Ma , Yan Zhang , Wei-hua Ma , Jing-jing Wang , Zhen-ya Tian , Guang-mei Chen , Ru-wen Li , Jin-hua Li , Jing-fang Yang , Zhong-shi Zhou

{"title":"Functional analysis of Ophraella communa Lesage OcomOBP11 in recognition of Ambrosia artemisiifolia L. volatiles","authors":"Yang Yue , Chao Ma , Yan Zhang , Wei-hua Ma , Jing-jing Wang , Zhen-ya Tian , Guang-mei Chen , Ru-wen Li , Jin-hua Li , Jing-fang Yang , Zhong-shi Zhou","doi":"10.1016/j.pestbp.2025.106392","DOIUrl":"10.1016/j.pestbp.2025.106392","url":null,"abstract":"<div><div>Odorant binding proteins (OBPs) play a critical role in insect survival by facilitating the detection of environmental odors. This study identified and characterized <em>OcomOBP11</em> in the leaf beetle <em>Ophraella communa</em> Lesage. Sequence and phylogenetic analyses demonstrated that <em>OcomOBP11</em> belongs to the Minus-C OBP family. Tissue-specific expression analysis revealed that <em>OcomOBP11</em> showed the highest relative expression in the testis. Functional assays demonstrated that OcomOBP11 has strong binding affinity to 24 of the 26 volatiles from <em>Ambrosia artemisiifolia</em> L., a primary host plant. Molecular docking showed that OcomOBP11 formed hydrophobic interactions with β-caryophyllene, α-pinene, camphene, octanal, and nonanal and hydrogen bonds with octanal and nonanal. RNAi-mediated knockdown of OcomOBP11 significantly reduced the electrophysiological (EAG) response and behavioral preference of <em>O. communa</em> for these ligands. These findings suggest that OcomOBP11 plays an important role in host plant localization, thus providing guidance for future biological invasion control strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106392"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of short-term ivermectin exposures on fruit flies

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106391

M. Yusuf Ali , Carl K. Namini , John M. Clark , Barry R. Pittendrigh , Si H. Lee , Kyong S. Yoon

{"title":"Impacts of short-term ivermectin exposures on fruit flies","authors":"M. Yusuf Ali , Carl K. Namini , John M. Clark , Barry R. Pittendrigh , Si H. Lee , Kyong S. Yoon","doi":"10.1016/j.pestbp.2025.106391","DOIUrl":"10.1016/j.pestbp.2025.106391","url":null,"abstract":"<div><div>A short-term ivermectin (IVM) exposure method was newly established to demonstrate effects of sublethal concentrations of IVM on the wild-type fruit fly, <em>Drosophila melanogaster</em>. Using a conventional glass-vial contact approach, exposures to IVM (0.01 to 1000 ppm) for 12 h durations or less were selected to assess the downstream impacts of short-term IVM exposures (STIEs) on fruit flies. Under these conditions, all female flies produced significantly higher levels of reactive oxygen species and malondialdehydes in their ovaries. Additionally, females treated with IVM for 12 h under the STIE conditions exhibited significantly increased levels of DNA damages in their ovaries. Despite the negative impacts described above, the mean percent hatchability values obtained from the eggs oviposited by the IVM-exposed females were not statistically different when compared to the hatchability of the unexposed females. Two concentrations (1 and 10 ppm) of IVM were selected to determine transgenerational effects following short-term IVM exposures. F1, F2 and F8 flies exposed to IVM showed significantly delayed developments (2.5–3.2, 2.5–3.0, and 0.9–1.3 days delayed, respectively). F5, F11 and F17 females showed significantly delayed IVM-induced sluggish behaviors in the presence of lethal IVM (1 %, <em>w</em>/<em>v</em>). F18 females transgenerationally exposed to 1 ppm IVM exhibited significantly increased levels of <em>Mrp1</em> (8.7-fold) and <em>Cyp6g2</em> (5.9-fold) transcripts compared to unexposed flies. Comparatively, F18 females transgenerationally exposed to 10 ppm IVM showed significantly increased levels of <em>Cyp9f2</em> (2.6-fold) transcripts. Current study clearly demonstrated the effects of sublethal IVM on parent and filial generations of fruit flies, providing an important step toward understanding development of IVM resistance under the STIE conditions.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106391"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bombyx mori nuclear polyhedrosis virus infection regulated by host glycosphingolipids

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106388

Feifei Zhu , Jindie Hong , Tingting Xue , Qi Tang , Qian Yu , Guohui Li , Shangshang Ma , Xiaoyong Liu , Shuhao Huo , Keping Chen

{"title":"Bombyx mori nuclear polyhedrosis virus infection regulated by host glycosphingolipids","authors":"Feifei Zhu , Jindie Hong , Tingting Xue , Qi Tang , Qian Yu , Guohui Li , Shangshang Ma , Xiaoyong Liu , Shuhao Huo , Keping Chen","doi":"10.1016/j.pestbp.2025.106388","DOIUrl":"10.1016/j.pestbp.2025.106388","url":null,"abstract":"<div><div>Glycosylation is an important post-translational modification commonly found in eukaryotes, and plays crucial roles in many biological activities. The silkworm <em>Bombyx mori</em> (<em>B. mori</em>), an important economic insect and a model organism in biology, has recently been found to be abundantly glycosylated. In this study, we established the role of silkworm glycosphingolipids (GSLs), the glycoconjugates formed by covalent attachment of a glycan to the lipid class of ceramide, during <em>B. mori</em> nuclear polyhedrosis virus (BmNPV) infection. The levels of cellular glycosphingolipids (GSLs), particularly the glucosylceramide (Glc-Cer) series, were modulated by targeting uridine diphosphate-glucose ceramide glycosyltransferase (UGCG), the enzyme responsible for Glc-Cer synthesis. Inhibiting UGCG activity by Genz-123346 (Genz), an inhibitor and substrate analogue of UGCG, reduced BmNPV binding, internalization, and viral protein expression in BmN cells. A general reduction in the cellular GSL contents was observed following Genz treatment. Overexpression of UGCG increased cellular GSL levels overall while still caused suppression in viral infection. It is postulated that GSLs are highly regulated membrane components that are crucial for viral entry, and disturbing the balance, either by increasing or decreasing cellular GSL components, alters membrane traffic and transport, which is unfavorable for viral infection. Therefore, highly regulated cellular GSLs are required for effective BmNPV infection. This study provides direct evidence linking GSL levels to BmNPV infection, offering new insights into the role of GSLs in viral infection.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106388"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0