Pesticide Biochemistry and Physiology最新文献

Quaternary ammonium cationic surfactants target Vfm quorum sensing to suppress the virulence of rice pathogen Dickeya oryzae. 季铵盐阳离子表面活性剂以Vfm群体感应为靶点抑制稻瘟病菌的毒力。

IF 4 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-11-01 Epub Date: 2025-08-05 DOI: 10.1016/j.pestbp.2025.106618

Longhui Luo, Lan-Tu Xiong, Xingnan Zhang, Di Zhu, Lian-Hui Zhang, Zi-Ning Cui

{"title":"Quaternary ammonium cationic surfactants target Vfm quorum sensing to suppress the virulence of rice pathogen Dickeya oryzae.","authors":"Longhui Luo, Lan-Tu Xiong, Xingnan Zhang, Di Zhu, Lian-Hui Zhang, Zi-Ning Cui","doi":"10.1016/j.pestbp.2025.106618","DOIUrl":"https://doi.org/10.1016/j.pestbp.2025.106618","url":null,"abstract":"The rice foot rot disease caused by Dickeya oryzae is an important bacterial disease that could cause tremendous economic losses. The virulence factor modulating cluster (Vfm) quorum sensing (QS) system, a major virulence regulatory mechanism conserved in the Dickeya genus, controls the production of zeamines and various extracellular cell wall degradation enzymes in D. oryzae. In this study, we targeted the Vfm QS system of D. oryzae strain EC1 to screen for chemical compounds capable of attenuating its virulence. Using a high-throughput vfmE-lux reporter assay, we screened over 2000 compounds and identified a series of long aliphatic chain compounds - quaternary ammonium cationic surfactants (QACs) that substantially reduced the vfmE promoter activity. Further analysis revealed that QACs blocked strain EC1 response to Vfm signals. Quantitative PCR results showed that the transcriptional expression of the vfm gene cluster and its downstream virulence genes, including those encoding production of phytotoxin zeamines and CWDEs were significantly downregulated. Consistent with their role in blocking Vfm signaling, QACs markedly inhibited the production of virulence determinant zeamines and suppressed the secretion of cellulase and pectinase in strain EC1. Infection assays demonstrated that these compounds substantially reduced the virulence of D. oryzae EC1 toward rice seeds and alleviated the soft rot symptoms in potato tubers. This is the first report that QACs are potent inhibitors of the D. oryzae Vfm QS system, illustrating a promising QS-targeted approach for the control of bacterial rice foot rot disease.","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"214 ","pages":"106618"},"PeriodicalIF":4.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First synergistic application of nanocarrier-loaded metaflumizone and parasitic wasps: A high-efficiency green pest control strategy. 首次协同应用纳米载体载metaflumizone和寄生蜂：一种高效的绿色害虫防治策略。

IF 4 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-11-01 Epub Date: 2025-08-07 DOI: 10.1016/j.pestbp.2025.106625

Shangyuan Wu, Qinhong Jiang, Leiyang Li, Ying Wei, Jinhong Zhou, Meizhen Yin, Jie Shen, Yufang Meng, Shuo Yan, Hu Li

{"title":"First synergistic application of nanocarrier-loaded metaflumizone and parasitic wasps: A high-efficiency green pest control strategy.","authors":"Shangyuan Wu, Qinhong Jiang, Leiyang Li, Ying Wei, Jinhong Zhou, Meizhen Yin, Jie Shen, Yufang Meng, Shuo Yan, Hu Li","doi":"10.1016/j.pestbp.2025.106625","DOIUrl":"https://doi.org/10.1016/j.pestbp.2025.106625","url":null,"abstract":"The improper use of chemical pesticides threatens ecosystems and human health, highlighting the need for sustainable alternatives. Nano-pesticides and biological control agents offer a solution, and their combination can reduce pesticide usage and improve pest control efficacy. This study utilized a star polycation (SPc) to prepare a metaflumizone nano-pesticide and combined it with the egg parasitoid (Telenomus remus) for synergistic pest management. SPc exhibited minimal toxicity to T. remus at working concentrations but caused mortality at extremely high doses, with an oral LC50 of 4.23 mg/mL. RNA-seq analysis revealed that the stress responses in T. remus induced by SPc included enhanced phagocytosis, exocytosis and energy synthesis. The SPc self-assembled with metaflumizone through hydrogen bonding and Van der Waals forces, reduced the particle size to the nanoscale (467.25 nm). The nano-pesticide increased stomach toxicity against Spodoptera litura by over 30 %, which showed no significant toxicity to T. remus eggs or adults and maintained parasitic capacity. The combined application of metaflumizone/SPc complex and T. remus achieved over 90 % pest control efficacy, providing a novel and effective strategy for sustainable pest management.","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"214 ","pages":"106625"},"PeriodicalIF":4.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Varroa destructor infestation amplifies imidacloprid vulnerability in Apis mellifera. 灭瓦螨侵染增加了蜜蜂吡虫啉的易损性。

IF 4 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-11-01 Epub Date: 2025-08-08 DOI: 10.1016/j.pestbp.2025.106616

Yinchen Wang, Xijie Li, Chunhui Miao, Chonghui Zhao, Jun Zhang, Yantao Pang, Junjie Li, Richan Fang, Xueyi Shen, Ying Lin, Tian Zhao, Mengqing Deng, Luansong Zhang, Hua Wang, Wanli Li, Jun Guo

{"title":"Varroa destructor infestation amplifies imidacloprid vulnerability in Apis mellifera.","authors":"Yinchen Wang, Xijie Li, Chunhui Miao, Chonghui Zhao, Jun Zhang, Yantao Pang, Junjie Li, Richan Fang, Xueyi Shen, Ying Lin, Tian Zhao, Mengqing Deng, Luansong Zhang, Hua Wang, Wanli Li, Jun Guo","doi":"10.1016/j.pestbp.2025.106616","DOIUrl":"https://doi.org/10.1016/j.pestbp.2025.106616","url":null,"abstract":"Honey bee health is affected by a variety of environmental factors, with Varroa destructor parasitism and pesticide exposure being important factors contributing to colony decline. In this study, we assessed the effects of V. destructor infestation in combination with imidacloprid exposure on honey bees. Our results showed that imidacloprid significantly reduced bee survival and that the synergistic effect of V. destructor and imidacloprid further exacerbated the risk of bee mortality. At the physiological level, both V. destructor and imidacloprid exposure increased GSTs and CYP450 enzyme activities, while PPO activity decreased, suggesting enhanced detoxification responses but impaired immune defences in bees. Meanwhile V. destructor infection significantly reduced the diversity of gut flora in newly emerged bees, while it had less effect on foraging bee. The abundance of Proteobacteria and Actinobacteriota decreased and the abundance of Firmicutes increased in the intestinal tract of newly emerged bees after infection with V. destructor. Meanwhile, Lactobacillus and Commensalibacter abundance increased, while Gilliamella, Snodgrassella and Bifidobacterium significantly decreased. Functional prediction analyses showed that the metabolic activity of newly emerged bees was enhanced after V. destructor infection, whereas the metabolic function of foraging bee did not change significantly. The study reveals the effects of harmful synergistic effects of V. destructor and imidacloprid on bee health and provides a scientific basis for bee conservation.","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"214 ","pages":"106616"},"PeriodicalIF":4.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycoside hydrolase Ma3360 mediates immune evasion by Metarhizium anisopliae in insects. 糖苷水解酶Ma3360介导金龟子绿僵菌的免疫逃避。

IF 4 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-11-01 Epub Date: 2025-08-05 DOI: 10.1016/j.pestbp.2025.106610

Yali Jiang, Hongwang Hu, Fen Liu, Xiangyu Hu, Qiongbo Hu, Jingjing Wang

{"title":"Glycoside hydrolase Ma3360 mediates immune evasion by Metarhizium anisopliae in insects.","authors":"Yali Jiang, Hongwang Hu, Fen Liu, Xiangyu Hu, Qiongbo Hu, Jingjing Wang","doi":"10.1016/j.pestbp.2025.106610","DOIUrl":"https://doi.org/10.1016/j.pestbp.2025.106610","url":null,"abstract":"Entomopathogenic fungi can precisely inhibit the cellular and humoral immune responses of host insects by secreting effector proteins, allowing them to overcome the innate immune barriers of their hosts. Nodule formation is an immune response primarily mediated by insect hemocytes, which can rapidly and efficiently capture invading pathogenic fungi in the hemocoel. However, the molecular mechanisms by which fungi inhibit insect nodule formation through the secretion of effector proteins remain unclear. First, we systematically observed the process of nodule induction and breakthrough by Metarhizium anisopliae. Following injection, the spores of M. anisopliae are quickly captured by hemocytes, and within 2 h, a significant number of hemocyte aggregates encapsulating the spores (early nodules) can be observed in the hemolymph. After 6 h, larger, melanized nodules can be collected from the hemocoel. After 18 to 24 h, the encapsulated spores begin to germinate and break through the nodules. By 36 to 48 h, the hyphae produce spores through budding, rapidly spreading and occupying the hemocoel. Subsequently, using Hemocytin, a key protein in insect nodule formation, as a mediator, we conducted pull-down assays, yeast two-hybrid experiments, surface plasmon resonance, and yeast secretion assays, discovering that a secreted glycoside hydrolase family 16 protein (Ma3360) in M. anisopliae acts as an effector that facilitates the breakthrough of nodules. Further functional studies indicated that Ma3360 also promotes the growth of M. anisopliae under stress conditions and disrupts the morphological structure of hemocytes. The results of this study reveal the molecular mechanisms by which pathogenic fungi utilize effector proteins to disrupt or evade the cellular immune responses of host insects.","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"214 ","pages":"106610"},"PeriodicalIF":4.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nlgalectin mediates insecticide susceptibility in Nilaparvata lugens via modulation of bacterial symbiont. 乳凝集素通过调节细菌共生体介导褐飞虱对杀虫剂的敏感性。

IF 4 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-11-01 Epub Date: 2025-08-07 DOI: 10.1016/j.pestbp.2025.106622

Jingbo Li, Yao Xu, Yue Wan, Tian Yan, Jianhong Li, Shun He, Hu Wan, Zhao Li, Guijian Zhang

{"title":"Nlgalectin mediates insecticide susceptibility in Nilaparvata lugens via modulation of bacterial symbiont.","authors":"Jingbo Li, Yao Xu, Yue Wan, Tian Yan, Jianhong Li, Shun He, Hu Wan, Zhao Li, Guijian Zhang","doi":"10.1016/j.pestbp.2025.106622","DOIUrl":"https://doi.org/10.1016/j.pestbp.2025.106622","url":null,"abstract":"Galectins are a family of carbohydrate-binding proteins known to maintain intestinal microbiota homeostasis. Emerging evidence suggests that the bacterial symbiont plays a role in modulating insecticide resistance in insect. However, whether galectins influence insecticide susceptibility through microbiota regulation remains unclear. In this study, we investigated endogenous galectin genes involved in regulating insecticide susceptibility in Nilaparvata lugens. Upon nitenpyram/sulfoxaflor exposure, the expression levels of Nlgal genes were significantly upregulated. qRT-PCR revealed higher Nlgal levels in susceptible strains, and their silencing reduced susceptibility to nitenpyram and sulfoxaflor. Concomitantly, the knockdown of Nlgal significantly increased total bacterial abundance, suggesting a potential link between Nlgal-mediated symbiont modulation and host insecticide susceptibility. These findings implicate Nlgal in insecticide susceptibility of N.lugens through putative interactions with bacterial symbionts, highlighting its potential as a novel target for resistance management.","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"214 ","pages":"106622"},"PeriodicalIF":4.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fenoxaprop-P-ethyl resistance in Alopecurus aequalis: Involvement of GSTF13 in addition to Ile-1781-Leu mutation. 白棘对苯丙醇- p-乙基的抗性：GSTF13和il -1781- leu突变的参与

IF 4 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-11-01 Epub Date: 2025-08-05 DOI: 10.1016/j.pestbp.2025.106614

You Zhan, Ziheng Cao, Jiale Qi, Youcheng Luo, Lifeng Hu, Lianyang Bai, Lang Pan

{"title":"Fenoxaprop-P-ethyl resistance in Alopecurus aequalis: Involvement of GSTF13 in addition to Ile-1781-Leu mutation.","authors":"You Zhan, Ziheng Cao, Jiale Qi, Youcheng Luo, Lifeng Hu, Lianyang Bai, Lang Pan","doi":"10.1016/j.pestbp.2025.106614","DOIUrl":"https://doi.org/10.1016/j.pestbp.2025.106614","url":null,"abstract":"Shortawn foxtail (Alopecurus aequalis Sobol.) is a challenging weed species to manage in wheat production systems globally. In prior research, we identified a field population of A. aequalis (Aa-R) that had developed resistance to the widely used acetolactate synthase (ALS)-inhibiting herbicide mesosulfuron-methyl. In this study, we found that the Aa-R population also exhibited significant resistance to the acetyl-CoA carboxylase (ACCase)-inhibiting herbicide fenoxaprop-P-ethyl and showed broad-spectrum resistance to other three ACCase-inhibiting herbicides, haloxyfop-P-methyl, clodinafop-propargyl, clethodim, and pinoxaden. Sequence analysis of the ACCase gene revealed the presence of a known resistance mutation (Ile-1781-Leu) in the Aa-R population. Pretreatment with the GST inhibitor 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) decreased the resistance to fenoxaprop-P-ethyl in the Aa-R population. We amplified an upregulated GST gene in the Aa-R population, designated AaGSTF13. Transgenic rice calli and seedlings overexpressing AaGSTF13 exhibited resistance specifically to fenoxaprop-P-ethyl, and might enhance reactive oxygen species (ROS) scavenging capacity. Further transcriptome analyses suggested that the expressions of genes associated with ROS scavenging was upregulated in transgenic plants. Our results indicate that AaGSTF13 enhances detoxification metabolism and could potentially enhances ROS scavenging in transgenic rice, which might contribute to enhanced herbicide resistance. These findings suggest that AaGSTF13 represents a promising candidate gene for the genetic improvement of new rice varieties under herbicide stress conditions.","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"214 ","pages":"106614"},"PeriodicalIF":4.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Divergent roles of AaOS1 and AaOS2 in procymidone resistance and pathogenicity of Alternaria alternata from tobacco. AaOS1和AaOS2在烟草互花霉抗性和致病性中的不同作用。

IF 4 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-11-01 Epub Date: 2025-08-06 DOI: 10.1016/j.pestbp.2025.106620

Jingqun Zhang, Liu-Ti Cai, Yangying Chen, Tianling Ma, Han-Cheng Wang, Chuanqing Zhang

{"title":"Divergent roles of AaOS1 and AaOS2 in procymidone resistance and pathogenicity of Alternaria alternata from tobacco.","authors":"Jingqun Zhang, Liu-Ti Cai, Yangying Chen, Tianling Ma, Han-Cheng Wang, Chuanqing Zhang","doi":"10.1016/j.pestbp.2025.106620","DOIUrl":"https://doi.org/10.1016/j.pestbp.2025.106620","url":null,"abstract":"Tobacco brown spot disease (TBSD), is a severe leaf disease caused by Alternaria alternata, and its management heavily relies on dicarboximide fungicides. This study analyzed procymidone, a dicarboximide fungicide, resistance in 96 strains of A. alternata isolated from tobacco in Guizhou Province. Results revealed a resistance frequency of 66.67 %, with low-resistance (ProLR) and high-resistance (ProHR) strains accounting for 54.17 % and 12.50 %, respectively. Compared to procymidone-sensitive strains, ProR isolates suffered significant fitness penalties, including impaired mycelial growth, spore production, and germination, despite retaining pathogenicity. Resistant strains also exhibited significantly enhanced sensitivity to NaCl, SDS, and Congo red. Sequencing analysis showed that ProHR strain GZA-28 existed G305K + M306I mutations in AaOS1 may potentially impact its function, and the molecular dynamics simulation further verified this, as evidenced by the reduced HAMP binding free energy from-249.78 kcal/mol in sensitive strains to-240.84 kcal/mol in GZA-28, resulting abnormal signal transduction function and endowing with procymidone resistance. Functional analysis of the HOG-MAPK pathway components AaOS1 and AaOS2 revealed divergent roles: AaOS1 deletion primarily enhanced DCFs resistance with moderate fitness costs, while AaOS2 deletion caused severe growth, sporulation, and virulence defects alongside resistance, highlighting AaOS2's critical role in basic physiology. This study integrates field resistance prevalence, fitness costs, a novel resistance mechanism (AaOS1 G305K/M306I), osmotic stress linkage, and the distinct functions of AaOS1/AaOS2, offering vital insights for understanding and managing procymidone resistance in TBSD.","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"214 ","pages":"106620"},"PeriodicalIF":4.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of G protein-coupled receptors (GPCRs) contributing to lambda-cyhalothrin resistance in Cydia pomonella. G蛋白偶联受体（gpcr）的过表达促进了田鼠对氯氟氰菊酯的抗性。

IF 4 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-11-01 Epub Date: 2025-07-31 DOI: 10.1016/j.pestbp.2025.106608

Yu-Xi Liu, Chao Hu, Zi-Nan Xia, Yan Wang, Yu-Ting Li, Ping Gao, Yun-Tong Lv, Jia Li, Xue-Qing Yang

{"title":"Overexpression of G protein-coupled receptors (GPCRs) contributing to lambda-cyhalothrin resistance in Cydia pomonella.","authors":"Yu-Xi Liu, Chao Hu, Zi-Nan Xia, Yan Wang, Yu-Ting Li, Ping Gao, Yun-Tong Lv, Jia Li, Xue-Qing Yang","doi":"10.1016/j.pestbp.2025.106608","DOIUrl":"https://doi.org/10.1016/j.pestbp.2025.106608","url":null,"abstract":"G protein-coupled receptors (GPCRs) constitute a diverse and crucial family of membrane receptors, regulating a wide array of physiological processes. Although the involvement of GPCR signaling pathways in modulating key genes associated with insecticide resistance has been documented in various insect species, the molecular mechanisms underlying GPCR-mediated resistance in Cydia pomonella remain largely unknown. To elucidate the molecular basis of lambda-cyhalothrin (LCT) resistance in C. pomonella, we performed comparative transcriptome sequencing on whole-body, head, and midgut tissues of larvae from a susceptible population (SS) and a laboratory-selected LCT-resistant population (LCR) derived from SS. Five GPCR candidates (CpGPCRs) exhibiting overexpression in LCR were identified, including two members of family A (CpGPCR4 and CpGPCR55), two of family B (CpGPCR68 and CpGPCR76), and one of family F (CpGPCR94). Spatiotemporal expression profiling revealed that these genes were predominantly expressed during the first-instar larval and pupal-adult stages, with notably high expression levels in the larval heads. Notably, all genes exhibited significantly elevated expression in the midgut of LCR larvae compared to SS larvae. RNA interference (RNAi)-mediated knockdown of CpGPCR4, CpGPCR55, and CpGPCR68 increased larval susceptibility to LCT, while knockdown of CpGPCR4 and CpGPCR55 significantly reduced larval survival rates. Furthermore, larvae treated with dsGPCR55 exhibited molting defects and a decline in 20-hydroxyecdysone (20E) titers. These findings demonstrate that CpGPCRs play essential roles in the survival and development of C. pomonella and are critically involved in mediating resistance to LCT. This study enhances our understanding of the molecular mechanisms driving insecticide resistance and identifies potential targets for the development of novel pest management strategies against C. pomonella.","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"214 ","pages":"106608"},"PeriodicalIF":4.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of microbial infection on key gene expression in the Toll signaling pathway and immune response in Myzus persicae. 微生物感染对桃蚜Toll信号通路关键基因表达及免疫应答的影响

IF 4 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-11-01 Epub Date: 2025-08-05 DOI: 10.1016/j.pestbp.2025.106611

Chao Zhang, Li He, Hong Yang, Maofa Yang

{"title":"Effects of microbial infection on key gene expression in the Toll signaling pathway and immune response in Myzus persicae.","authors":"Chao Zhang, Li He, Hong Yang, Maofa Yang","doi":"10.1016/j.pestbp.2025.106611","DOIUrl":"https://doi.org/10.1016/j.pestbp.2025.106611","url":null,"abstract":"The Toll signaling pathway serves as a crucial regulatory mechanism in the insect innate immune system, playing a pivotal role in defending against pathogenic microorganisms. However, the specific functions of aphids' unique immune system and Toll signaling pathway remain poorly understood. In this study, we systematically analyzed 12 key genes associated with the Toll signaling pathway in Myzus persicae. Our findings revealed that Toll pathway-related genes exhibit high expression levels in the hemolymph and midgut tissues of the M. persicae. Furthermore, these genes were significantly upregulated following exposure to Staphylococcus aureus, Escherichia coli, and Beauveria bassiana. Through RNA interference (RNAi) coupled with microbial infection assays, we demonstrated that knockdown of Toll pathway-related genes substantially compromised the antibacterial capacity of M. persicae. Notably, we identified distinct functional specializations among these genes: MpMyd88 and MpIRAK4 were critical for resistance against S. aureus infection, while MpPelle, MpP38, MpJNK, and MpAP primarily mediated defense against B. bassiana. Additionally, genetic knockdown experiments revealed that MpMyd88, MpIRAK4, MpPelle, and MpCactus significantly downregulated the expression of key immune factors (MpNedd8, MpLys1, MpLys2, and MpLys3), though interestingly not MpPPO1 and MpPPO2. These results not only underscore the vital role played by the Toll signaling pathway in aphid immune defense but also provide essential molecular targets along with theoretical support for developing innovative aphid control strategies based on synergistic effects between RNAi and microbial.","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"214 ","pages":"106611"},"PeriodicalIF":4.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered bacterium Pseudomonas abietaniphila BHJ04-BLG4 controls pine wilt disease via nematocidal activity and induction of host systemic resistance 工程细菌abietaniphila Pseudomonas BHJ04-BLG4通过杀线虫活性和诱导宿主系统抗性来控制松树枯萎病

IF 4 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-10-01 DOI: 10.1016/j.pestbp.2025.106729

Da Li , Menglu Yu , Lvxin Huang , Jianren Ye , Xiaoqin Wu

{"title":"Engineered bacterium Pseudomonas abietaniphila BHJ04-BLG4 controls pine wilt disease via nematocidal activity and induction of host systemic resistance","authors":"Da Li , Menglu Yu , Lvxin Huang , Jianren Ye , Xiaoqin Wu","doi":"10.1016/j.pestbp.2025.106729","DOIUrl":"10.1016/j.pestbp.2025.106729","url":null,"abstract":"<div><div>Pine wilt disease (PWD), caused by Bursaphelenchus xylophilus, is a destructive global forest disease for which microbial control offers promising potential. Pseudomonas abietaniphila BHJ04, an endophytic bacterium from Pinus massoniana, has been shown to induce host resistance. To enhance its biocontrol efficacy against PWD, we engineered this strain to improve nematicidal activity while maintaining resistance induction. An exogenous nematicidal gene encoding the extracellular alkaline serine protease BLG4 was introduced into BHJ04 by conjugation transfer, generating strain BHJ04-BLG4. Colonization in P. massoniana stems peaked 15 days post-inoculation (2.06 × 104 copies/μL). In vitro assays demonstrated that purified BLG4 degraded nematode cuticles and killed nematodes (IC50 = 35.98 μg/mL), while crude lysate supernatant from 48 h cultures achieved 100 % nematicidal activity. In greenhouse trials, BHJ04-BLG4 reduced PWD incidence to 40 % (control efficacy: 86.66 %). RT-qPCR showed significant induction of host defense genes: dehydration-responsive protein (315.06-fold at 48 h), and chitinase, PR-2 (β-1,3-glucanase), and PR-5 (thaumatin-like protein) genes (7.43–34.26-fold within 24–48 h). Preliminary ecological safety tests indicated that BHJ04-BLG4 caused no disease symptoms in tobacco, alfalfa, or P. thunbergii. These findings suggest that BHJ04-BLG4 confers enhanced resistance to PWD through synergistic mechanisms of direct nematicidal activity and host systemic resistance induction. This engineered strain represents a novel and sustainable biocontrol strategy for PWD management.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"216 ","pages":"Article 106729"},"PeriodicalIF":4.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145222966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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