Pesticide Biochemistry and Physiology最新文献

{"title":"Comparation of pheromone-binding proteins 1 and 2 of Spodoptera frugiperda in perceiving the three sex pheromone components Z9-14:Ac, Z7-12: Ac and Z11-16: Ac","authors":"Liming Hu ,&nbsp;Taoli Zhang ,&nbsp;Qingjun Wu ,&nbsp;Kangyuan Liang ,&nbsp;Guohui Yu ,&nbsp;Muyang He ,&nbsp;Dasong Chen ,&nbsp;Xiangning Su ,&nbsp;Yuping Zhang ,&nbsp;Zhenfei Zhang ,&nbsp;Jianmei Shen","doi":"10.1016/j.pestbp.2024.106183","DOIUrl":"10.1016/j.pestbp.2024.106183","url":null,"abstract":"<div><div>Pheromone-binding proteins (PBPs) are mainly responsible for binding and transporting hydrophobic pheromone molecules across the aqueous sensilla lymph to the receptor proteins. The preference of each PBP is believed to be different for each pheromone component within a single species. Significantly higher expression level of PBP1 and PBP2 in the male antennae of <em>Spodoptera frugiperda</em> suggesting that <em>Sfru</em>PBP1 and <em>Sfru</em>PBP2 might play important roles in pheromone perception. However, the preference of these two PBP to the three main pheromone components Z9-14: Ac, Z7-12: Ac and Z11-16: Ac have not been determined. In this study, a fluorescence competitive binding assay revealed that the binding intensities of <em>Sfru</em>PBP1 and <em>Sfru</em>PBP2 to Z9-14: Ac or Z7-12: Ac was comparable. We then used the CRISPR/Cas9 system to individually or simultaneously knock out PBP1 and PBP2 in <em>S.frugiperda</em>. The result of courtship behavior indicated that <em>Sfru</em>PBP1 and <em>Sfru</em>PBP2 were indispensable and played equal roles in perceiving the pheromones Z9-14: Ac and Z7-12: Ac for orientation, wing vibration, and hair-pencil display. Compared with Z9-14:Ac and Z7-12: Ac, Z11-16: Ac showed higher or medium binding intensities with <em>Sfru</em>PBP1 and <em>Sfru</em>PBP2 but played a minor role in inducing the wing vibration behavior. The results of this study are valuable for elucidating the mechanisms involved in sex pheromone perception and may facilitate the development of PBP-targeted pest control techniques.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"206 ","pages":"Article 106183"},"PeriodicalIF":4.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142748722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol activity and action mechanism of Pseudomonas aurantiaca ST-TJ4 against Verticillium dahliae, the causal agent of Acer truncatum wilt","authors":"Liang-Liang Hou ,&nbsp;Wei-Liang Kong ,&nbsp;Xiao-Qin Wu","doi":"10.1016/j.pestbp.2024.106224","DOIUrl":"10.1016/j.pestbp.2024.106224","url":null,"abstract":"<div><div><em>Acer truncatum</em> wilt caused by <em>Verticillium dahliae</em> is a severe soilborne disease that poses a threat to the cultivation of this plant in China. The present study explored the biocontrol efficiency and underlying antagonistic mechanism of <em>Pseudomonas aurantiaca</em> ST-TJ4 against <em>V. dahliae</em>. In vitro, strain ST-TJ4 exhibited excellent inhibitory effects on <em>V. dahliae</em>, causing mycelial deformation. This strain significantly suppressed the production of <em>V. dahliae</em> conidia and microsclerotia<em>.</em> Moreover, the application of ST-TJ4 reduced the incidence of <em>Verticillium</em> wilt in <em>A. truncatum</em> saplings in both the prevention group and the cure group. Comparative transcriptomic analyses revealed that ST-TJ4 induced differential expression of numerous genes in <em>V. dahliae</em>, most of which were downregulated. These differentially expressed genes were associated with cell wall-degrading enzyme activity, sterol biosynthetic processes, glutathione S-transferase activity, iron ion and sugar metabolism, and oxidoreductase activity. Further transcriptomic analyses of physiological indices indicated that ST-TJ4 significantly inhibited the synthesis of pectin lyase, <em>endo</em>-β-1,4-glucanase, melanin and soluble sugars of <em>V. dahliae</em> and had a stronger inhibitory effect under iron deficiency. Taken together, these data highlight <em>P. aurantiaca</em> ST-TJ4 as a promising biocontrol agent against <em>A. truncatum</em> Verticillium wilt.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106224"},"PeriodicalIF":4.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C-type lectin 9 participates in the immune response, development and reproduction of Tribolium castaneum","authors":"Chengjun Li,&nbsp;Huayi Ai,&nbsp;Biao Zhang,&nbsp;Xiaoqiao Huang,&nbsp;Bin Li","doi":"10.1016/j.pestbp.2024.106223","DOIUrl":"10.1016/j.pestbp.2024.106223","url":null,"abstract":"<div><div>C-type lectins (CTLs), as a large family of pattern recognition receptors (PRRs), have been reported to be involved in bacterial infection, but the role of CTLs in development has been poorly understood in insects. The orthologues of <em>Tribolium castaneum</em> CTL9 (TcCTL9) have been identified among insects, but its functions were currently unclear. Therefore, we performed functional analysis of TcCTL9 in this study. Our results indicated that TcCTL9 could bind to bacteria through lipopolysaccharide and peptidoglycan, and agglutinate Gram-positive and Gram-positive bacteria in a Ca²⁺-dependent manner. Silencing <em>TcCTL9</em> reduced the immune resistance to <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>, decreased the expression of antimicrobial peptides and prophenoloxidase, and inhibited the phenoloxidase activity. These data suggested that TcCTL9 functioned in the immune response via the Toll and IMD pathways and prophenoloxidase system. During development, <em>TcCTL9</em> had high expression in the periods of egg to larva and pupa to adult, and knockdown of <em>TcCTL9</em> suppressed the metamorphosis, egg production and hatchability, and ovary development through ecdysone and juvenile hormone pathways in <em>T. castaneum</em>. This study comprehensively clarified the functions of TcCTL9 orthologues in insects and provided the theoretical basis for developing novel targets of pesticides.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106223"},"PeriodicalIF":4.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The function of HgLac in Heterodera glycines and its potential as a control target","authors":"Dongya Wang ,&nbsp;Fangcao Wu ,&nbsp;Xiongbiao Xu ,&nbsp;Deliang Peng ,&nbsp;Yuxi Duan ,&nbsp;Huan Peng ,&nbsp;Haiyan Wu","doi":"10.1016/j.pestbp.2024.106225","DOIUrl":"10.1016/j.pestbp.2024.106225","url":null,"abstract":"<div><div>The soybean cyst nematode (SCN; <em>Heterodera glycines</em>) is one of the most devastating pathogens for soybean production. The second stage juvenile (J2) invades the host root, develops and form white females which then become brown cysts enter the soil. The brown cyst wall plays a key role in protecting inside eggs from adverse environmental conditions. However, the function of cyst wall tanning (sclerotization and pigmentation) in nematodes is not clear. A browning-related gene discovered from the whole-genome sequencing was cloned and characterized in this study, the gene was confirmed to be the laccase gene and was named <em>HgLac</em>. <em>HgLac</em> mRNA and HgLac protein was detected in the epidermis of juveniles using <em>in situ</em> hybridization and immunolocalization techniques. The <em>HgLac</em> expression level was greater in fourth-stage juveniles (J4s) than in the other stages. Knockdown of <em>HgLac</em> by <em>in vitro</em> RNA interference (RNAi) significantly decreased the infectivity, development and reproduction of J2s but had no effect on cyst wall tanning. Further research revealed that <em>HgLac</em> expression in nematodes was significantly suppressed by 35.41–59.17 % through <em>in planta</em> RNAi, 52.96–58.19 % females could not tan successfully, and the female wall was very soft and fragile, with a low egg hatching rate (1.33 %), which was significantly lower than that of normal females (68.85 %). These results indicate that <em>HgLac</em> plays a key role in cyst wall tanning and suppressing the development and reproduction of the SCN, which provides new ideas for the use of this gene as a target to control SCN.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106225"},"PeriodicalIF":4.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of the transfluthrin effects on fertility and pregnancy outcomes: An in-vivo study in rat","authors":"Akhilesh Mishra ,&nbsp;Gayatri Dewangan ,&nbsp;Megh Singh Dhakad ,&nbsp;Subash Chandra Sonkar ,&nbsp;Jasbir Dalal ,&nbsp;Sunita Pradhan ,&nbsp;Divakar Sharma ,&nbsp;Vandana Roy ,&nbsp;Bidhan Chandra Koner","doi":"10.1016/j.pestbp.2024.106220","DOIUrl":"10.1016/j.pestbp.2024.106220","url":null,"abstract":"<div><div>Transfluthrin (TFL) is a rapid-acting pyrethroid insecticide, commonly used worldwide, however its overuse and or misuse has been associated with adverse effects on human health. This study was designed to investigate the impact of TFL on fertility and pregnancy in Sprague–Dawley rats exposed orally and by inhalation. In this study, 48 adult Sprague–Dawley rats of 6–8 months age group were randomly selected with equal numbers of males and females. Each group was housed separately in a standard laboratory condition and their daily consumption of food and water, along with weight of each group were monitored. The blood was collected from each rat on 0 (zero) and 85th day of oral and inhalation exposure to TFL to determine the hormone levels by ELISA. The findings revealed that TFL exposure through oral and inhalation route reduced hormones level in serum such as testosterone, follicle-stimulating hormone, luteinizing hormone, progesterone, estrogen, and prolactin in both male and female rats. In conclusion, current research suggest the negative effects of TFL exposure on fertility, adversely affecting pregnancy outcomes, reducing the mating rate, and lowering the number of pups. It also stresses the significance of controlling its use to prevent any negative consequences.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106220"},"PeriodicalIF":4.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of the β-N-acetylhexosaminidase genes by RNA interference inhibited the molting and increased the mortality of the white-backed planthopper, Sogatella furcifera","authors":"Pan-Pan Guo ,&nbsp;Xi-Bin Yang ,&nbsp;Hong Yang ,&nbsp;Cao Zhou ,&nbsp;Gui-Yun Long ,&nbsp;Dao-Chao Jin","doi":"10.1016/j.pestbp.2024.106216","DOIUrl":"10.1016/j.pestbp.2024.106216","url":null,"abstract":"<div><div><em>β</em>-N-Acetylglucosaminidases and/or <em>β</em>-<em>N</em>-acetylhexosaminidases (NAGs / Hexes) are crucial exonucleases, playing a crucial role in the insect molting process. SfHex3 and SfHex4 contain conserved catalytic domains of GH20 and GH20b, clustered into NAG2 and NAG1 group, respectively. <em>SfHex3</em> and <em>SfHex4</em> were mainly highly expressed in the 4th–5th instar nymphs, as well as in the integument and ovary. The expression level of <em>SfHex3</em> gradually decreased in male and female adults, and <em>SfHex4</em> on the first day of female was significantly higher than that on the first day of male. In addition, RNA interference (RNAi) results demonstrated that the downregulation of <em>SfHex3</em> and <em>SfHex4</em> expression in 5th-instar nymphs resulted in failed molting, and a high mortality. Furthermore, after RNAi with <em>SfHex3</em> and <em>SfHex4</em>, the transcript levels on key genes of the chitin metabolism pathway (<em>SfCHS1</em>, <em>SfCHS1a</em>, <em>SfCHS1b</em>, <em>SfTRE1</em>, <em>SfTRE2</em>, <em>SfCht5</em>, and <em>SfCht7</em>) were significantly decreased compared to the control group. Meanwhile the expression levels of <em>SfHex3</em> and <em>SfHex4</em> were up-regulated after 6 h and 12 h of 20E treatment. And the transcription levels of <em>SfHex3</em> and <em>SfHex4</em> were significantly inhibited at nitenpyram LC₂₀, LC₅₀, and LC₉₀ after 96 h of treatment, in 3rd nymphs of <em>Sogatella furcifera</em>. In conclusion, <em>SfHex3</em> and <em>SfHex4</em> play important roles in the nymphal development of <em>S. furcifera</em>, contributing to the molting process from nymph to adult. This study not only enhances our understanding of the nitenpyram in pest control, but also provides a foundation for the development of new control strategies using RNAi to targeting <em>SfHex3</em> and <em>SfHex4</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106216"},"PeriodicalIF":4.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution, frequency and molecular basis of penoxsulam, metamifop and florpyrauxifen-benzyl resistance in Echinocloa spp. from rice fields across Jiangsu Province, China","authors":"Wei Deng ,&nbsp;Hanqi Yin ,&nbsp;Zhixun Ge ,&nbsp;Sai Yao ,&nbsp;Jiawen Wu ,&nbsp;Axiu Zhu ,&nbsp;Qian Yang ,&nbsp;Shuzhong Yuan","doi":"10.1016/j.pestbp.2024.106218","DOIUrl":"10.1016/j.pestbp.2024.106218","url":null,"abstract":"<div><div>Barnyard grasses (<em>Echinochloa</em> spp.) are the most troublesome weeds in rice ecosystems of Jiangsu Province, China, and long-term use of herbicides inevitably cause the development of resistance. In this study, 122 field-sampled populations were tested for resistance to penoxsulam (PEN), metamifop (MET), florpyrauxifen-benzyl, (FPB), propanil, and tripyrasulfone. Field-dose assays revealed that 51, 7, and 6 populations have either developed or were developing resistance to penoxsulam, metamifop and florpyrauxifen-benzyl, and all 122 populations were still sensitive to propanil and tripyrasulfone. Dose-response assays identified that 41.8 % of populations exhibited 6- to 830-fold resistance to PEN; 5.7 % of populations displayed 2.2- to 13.9-fold resistance to MET; and 4.9 % of populations showed 2.4- to 11.1-fold resistance to FPB. Trp-574-Leu mutations in <em>EcALS1 or EcALS3</em> were found in 18.0 % of populations. Trp-2027-Cys, Cys-2088-Arg, and Gly-2096-Ala mutations in <em>EcACC1, EcACC3,</em> or <em>EcACC5</em> were detected in 4.1 % of populations, and no known resistance-associated target-site gene mutations were confirmed in the rest of herbicide-resistant populations. Furthermore, the P450 inhibitor malathion and GST inhibitor NBD-Cl significantly reduced the resistance to three herbicides in 38.5 % of populations, especially those without the target-site gene mutations, indicating both target-site mutations and P450s and/or GSTs-mediated metabolisms play important roles in herbicide resistance evolution in <em>Echinochloa</em> spp. These findings are helpful to formulate an appropriate strategy for effective and sustainable control of <em>Echinochloa</em> spp. by mixtures of herbicide with different modes of action and mitigate weed resistance evolution.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106218"},"PeriodicalIF":4.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of various physical and chemical stress conditions on the infectivity and survival of Heterorhabditis indica and Steinernema feltiae: Relationship with lipid oxidative stress","authors":"Lenin González-Paz ,&nbsp;Carla Lossada ,&nbsp;Dayrana Mora ,&nbsp;Edgar Portillo ,&nbsp;Ernesto San-Blas","doi":"10.1016/j.pestbp.2024.106217","DOIUrl":"10.1016/j.pestbp.2024.106217","url":null,"abstract":"<div><div>Entomopathogenic nematodes (EPNs) of the genera <em>Heterorhabditis</em> and <em>Steinernema</em> represent an alternative for the biological control of insects. The limited half-life of EPNs is still one of the most concerning issues in their commercialization. Lipid peroxidation (LPO) caused by reactive oxygen species (ROS) may be one of the most important causes of loss of infectivity and survival of EPNs when exposed to various physicochemical stress conditions (temperature, pH, hypoxia and osmotic pressure). Because LPO generates free radicals (FRs), it can trigger membrane peroxidation and lipid energy reserves of EPNs. However, in EPNs there is no data on the role of LPO on their physiology, making strategies for the conservation of derived biopreparations difficult. In this sense, the influence of LPO on the species of EPNs <em>S. feltiae</em> and <em>H. indica</em> under various conditions of physicochemical stress was studied. In both EPNs, the proposed stress conditions altered infectivity and survival over time, generating ROS associated with LPO with a variable tolerance depending on the species, type and time of exposure to stress. A relationship was observed between the LPO induced by stress conditions and infectivity-survival.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106217"},"PeriodicalIF":4.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fomesafen induced ecotoxicological responses, bioaccumulation, and implications for soil ecosystems remediation in three ecotypes earthworms","authors":"Yan Ge,&nbsp;Siqi Wang,&nbsp;Siqi Miao,&nbsp;Dongxing Zhou","doi":"10.1016/j.pestbp.2024.106215","DOIUrl":"10.1016/j.pestbp.2024.106215","url":null,"abstract":"<div><div>Fomesafen (FSA) is a herbicide commonly used in soybean fields, but its long half-life in the soil can pose pollution risks to the soil ecosystem. Earthworms, which have an indicative function for soil health and play a vital role in maintaining soil ecological functions, have not been fully studied in terms of their susceptibility to FSA. This study examined the effects of different concentrations of FSA on three ecotypes of earthworms (<em>Eisenia fetida</em> (epigeic), <em>Metaphire guillelmi</em> (anecic), and <em>Aporrectodea caliginosa</em> (endogeic)) and found varying trade-off strategy of their growth and reproduction. When subjected to FSA stress, <em>E. fetida</em> prioritized reproduction, <em>A. caliginosa</em> prioritized growth, while <em>M. guillelmi</em> maintained consistent growth and reproduction, effectively allocating the acquired biomass to reproduction. The study also analyzed the oxidative stress response, gene expression, and an integrated biomarker response. The strongest induction performance of the three ecotypes of earthworms was in the expression of antioxidant enzymes such as CAT, POD, SOD, and <em>P450</em> expression, while the performance was significantly different in AChE activity, <em>AChE</em> and <em>HSP70</em> expression, which may lead to differences in toxicity and detoxification mechanisms of earthworms in different ecotypes. The partial least squares-path model (PLS-PM) also showed that the three ecotypes of earthworms exhibited different toxicity, detoxification and regulatory pathways under FSA stress. <em>M. guillelmi</em> had the highest IBRv2 index (28.67–42.90). At the same time, the FSA bioaccumulation tended to be stable with increasing concentration, and its main detoxification ability (GST activity and <em>P450</em> expression) could still maintain the same level as CK at high concentrations, indicating that it is very likely to be a potential earthworm type for FSA remediation. This study firstly provides a comprehensive assessment of FSA's toxic effects on different ecotypes of earthworms and highlights the importance of identifying potential resistant species for soil remediation.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106215"},"PeriodicalIF":4.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of ATP-binding cassette transporters involved in chlorantraniliprole tolerance of model insect Drosophila melanogaster and agricultural pest Spodoptera frugiperda","authors":"Lixiang Wang ,&nbsp;Bin Wen ,&nbsp;Shaoyi Guo ,&nbsp;Yujie Han ,&nbsp;Zhongyuan Deng ,&nbsp;Qian Ding ,&nbsp;Xianchun Li","doi":"10.1016/j.pestbp.2024.106212","DOIUrl":"10.1016/j.pestbp.2024.106212","url":null,"abstract":"<div><div>ATP-binding cassette (ABC) transporter family is one of the largest transporter families, which plays an important role in insecticide tolerance. In this study, we found that the ABC transporter inhibitor verapamil could significantly enhance the toxicity of chlorantraniliprole (CHL) to the model insect <em>Drosophila melanogaster</em>. Forty-six ABC transporter genes of <em>D. melanogaster</em> were knocked down through the <em>daughterless</em>-<em>GAL4</em> (<em>Da</em>-<em>GAL4</em>) strain. The subsequent bioassay result showed that <em>D. melanogaster</em> with <em>DmCG5772</em>, <em>DmCG1494</em>, and <em>DmCG5853</em> genes silencing significantly increased mortality after CHL treatment. Based on the genome of the fall armyworm (FAW), three genes with the best hits were identified, and <em>SfABCA1</em> (XM_035576510.2) and <em>SfABCG10</em> (XM_035577893.2) were successfully cloned. Spatiotemporal expression pattern analysis showed that <em>SfABCA1</em> and <em>SfABCG10</em> were both highly expressed in adult and pupal stages. Hemolymph was also a tissue with high expression of these two genes. LC₁₀ dose of CHL could induce the expression levels of <em>SfABCA1</em> and <em>SfABCG10</em>, with <em>SfABCG10</em> upregulated 8-fold after 48 h of CHL treatment. Furthermore, overexpression of <em>SfABCA1</em> and <em>SfABCG10</em> increased the viability of <em>Sf9</em> cell under CHL treatment. Our findings indicate that <em>SfABCA1</em> and <em>SfABCG10</em> might associate with the tolerance of CHL in <em>S. frugiperda</em>. These results are not only helpful in understanding the role of ABC transporters in CHL tolerance of other agricultural pests, but also lay a theoretical foundation for delaying the development of CHL resistance in pest management.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"206 ","pages":"Article 106212"},"PeriodicalIF":4.2,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}