Pesticide Biochemistry and Physiology最新文献

{"title":"Corrigendum to “Transcriptome analysis reveals the pheromone synthesis mechanism and mating response in Monochamus saltuarius (Coleoptera, Cerambycidae)” [Pesticide Biochemistry and Physiology, 2024, 203, 105975]","authors":"Yiming Niu, Ye Chi, Yabei Xu, Sainan Zhang, Fengming Shi, Yuxuan Zhao, Meng Li, Shixiang Zong, Jing Tao","doi":"10.1016/j.pestbp.2026.107297","DOIUrl":"10.1016/j.pestbp.2026.107297","url":null,"abstract":"","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"223 ","pages":"Article 107297"},"PeriodicalIF":4.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148892173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triphenyltin-induced hepatotoxicity in zebrafish larvae: A multi-omics insight into lipid metabolism disruption and inflammation","authors":"Yu Zhang,&nbsp;Mengjiao Li,&nbsp;Wenyi Li,&nbsp;Xiyue Liu,&nbsp;Hongru Liu,&nbsp;Xinxin Deng,&nbsp;Tingting Qian,&nbsp;Hui Liu,&nbsp;Li Wang","doi":"10.1016/j.pestbp.2026.107240","DOIUrl":"10.1016/j.pestbp.2026.107240","url":null,"abstract":"<div><div>Triphenyltin (TPT) is a highly toxic organotin fungicide, widely found in all environments. Previous studies have demonstrated that TPT exposure causes hepatotoxicity and alters lipid profiles in livers and sera, however, the underlying mechanism between TPT-induced dysregulation of lipid metabolism and liver injury remains unclear, prompting us to investigate how TPT disturb lipid metabolic pathways to drive hepatic toxicity. This study used a multi-omics approach to investigate molecular responses associated with TPT-induced liver injury in zebrafish larvae. Zebrafish larvae were exposed to 15, 30 and 60 nmol/L TPT for 72 h. Transgenic zebrafish lines <em>Tg (fabp10a: DsRed)</em> and <em>Tg (lyz: DsRed)</em> were used to observe changes in liver area and inflammatory responses. The structural integrity of the liver was examined using HE staining and transmission electron microscope, while the presence of lipids in the zebrafish was assessed using Oil Red O, Nile Red and Sudan Black B staining. Transcriptomic and metabolomic analyses were conducted using whole-larvae samples to identify molecular pathways associated with the observed liver injury. The results indicate that TPT exposure causes liver inflammation and lipid accumulation. Differentially expressed genes and metabolites were primarily enriched in pathways related to PPAR signaling pathway, fatty acid metabolism and inflammation. RT-qPCR validation showed that changes in the expression of genes related to lipid metabolism were consistent with the omics results. In summary, TPT can cause liver injury in zebrafish by disrupting lipid metabolism and inflammation pathways. This study provides a theoretical basis for elucidating the mechanisms of TPT hepatotoxicity and for assessing the environmental risks of organotin compounds.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"222 ","pages":"Article 107240"},"PeriodicalIF":4.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148579771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Evolution of pyraclostrobin resistance in Botrytis cinerea from Xinxiang City of China and its targeted management via RNA interference” [Pesticide Biochemistry and Physiology 221 (2026) 107105]","authors":"Feng Zhou,&nbsp;Haichuan Su,&nbsp;Huifang Jia,&nbsp;Tiancheng Liu,&nbsp;Ziyang Li,&nbsp;Luxun Liu,&nbsp;Yanfen Wang,&nbsp;Weiguo Li,&nbsp;Zhongke Sun,&nbsp;Chengwei Li,&nbsp;Runqiang Liu","doi":"10.1016/j.pestbp.2026.107246","DOIUrl":"10.1016/j.pestbp.2026.107246","url":null,"abstract":"","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"222 ","pages":"Article 107246"},"PeriodicalIF":4.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148579818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DDT biotransformation by a delta-class glutathione S-transferase underlies tolerance in honey bees","authors":"Huali Song,&nbsp;Xiangyou Tang,&nbsp;Jingsong Hu,&nbsp;Jing Zhang,&nbsp;Mingyu Song,&nbsp;Yaohui Li,&nbsp;Yuting Ma,&nbsp;Zhengang Ma,&nbsp;Zeyang Zhou,&nbsp;Jinshan Xu","doi":"10.1016/j.pestbp.2026.107258","DOIUrl":"10.1016/j.pestbp.2026.107258","url":null,"abstract":"<div><div>Bees are key global pollinators but face long-term exposure to pesticide residues. Although the western honey bee (<em>Apis mellifera</em>) shows tolerance to the persistent organic pollutant DDT, the underlying molecular basis remains unclear. Glutathione S-transferases (GSTs) are important detoxification enzymes in bees, yet direct evidence for their involvement in pesticide metabolism remains limited. In insects, Delta- and Epsilon-class GSTs are typically associated with xenobiotic detoxification. However, the <em>A. mellifera</em> genome encodes only a single Delta-class GST, <em>AmGSTD1</em>, and lacks the Epsilon class, suggesting that <em>AmGSTD1</em> may play an important role in xenobiotic detoxification in honey bees. Here, we combined genetic, biochemical, and computational analyses to investigate the role of <em>AmGSTD1</em> in DDT detoxification. Silencing of <em>AmGSTD1</em> in <em>A. mellifera</em> increased susceptibility to DDT stress, whereas transgenic expression of <em>AmGSTD1</em> in <em>Drosophila melanogaster</em> conferred increased DDT tolerance. Enzyme kinetics and HPLC analyses demonstrated that recombinant AmGSTD1 protein catalyzed the biotransformation of DDT to DDE, while molecular docking further supported the interaction between AmGSTD1 protein and DDT. AmGSTD1 protein was also associated with alleviation of oxidative stress under DDT exposure. Furthermore, the A170V mutation was identified as an important factor affecting the detoxification activity of the AmGSTD1 protein. Overall, these findings provide functional evidence that AmGSTD1 protein contributes to DDT detoxification through both xenobiotic biotransformation and oxidative stress regulation. This study improves our understanding of the molecular mechanisms underlying pesticide tolerance in honey bees.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"222 ","pages":"Article 107258"},"PeriodicalIF":4.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148579841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to Engineering Bacillus thuringiensis Cyt1Aa to function as a surrogate receptor of Cry1A lepidopteran insecticidal proteins, Pesticide Biochemistry and Physiology 219 (2026) 106970","authors":"Nathaly Alexandre do Nascimento,&nbsp;Mary Carmen Torres-Quintero,&nbsp;Blanca Ines García-Gómez,&nbsp;Isabel Gómez,&nbsp;Sabino Pacheco,&nbsp;Alejandra Bravo,&nbsp;Mario Soberón","doi":"10.1016/j.pestbp.2026.107201","DOIUrl":"10.1016/j.pestbp.2026.107201","url":null,"abstract":"","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"222 ","pages":"Article 107201"},"PeriodicalIF":4.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148579832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABCG and ABCH transporters at the interface of cuticular lipid transport and insecticide handling","authors":"Jian-Wen Qiao,&nbsp;Li Li,&nbsp;Syed Husne Mobarak,&nbsp;Tong-Xian Liu,&nbsp;Bing-Jin Wu","doi":"10.1016/j.pestbp.2026.107255","DOIUrl":"10.1016/j.pestbp.2026.107255","url":null,"abstract":"<div><div>ATP-binding cassette (ABC) transporters are widely associated with insecticide resistance, yet the physiological links between endogenous transport and xenobiotic handling remain incompletely resolved for ABCG and arthropod-associated ABCH subfamilies. Unlike many canonical full ABC transporters, ABCG and ABCH proteins usually have reverse half-transporter topology and require dimeric assembly, creating substrate-recognition spaces that can support lipid movement, pigment precursor routing, cuticular barrier formation, and chemical-response phenotypes. Here, we synthesize structural, physiological, and toxicological evidence from insects and mites to evaluate how these transporters may connect cuticular lipid homeostasis with insecticide handling. Recent structural work on <em>Tribolium castaneum</em> TcABCH-9C supports an ATP-coupled squeeze-pump model for hydrophobic substrates, including ceramide-like lipids and fenoxycarb. This model provides an important mechanistic framework, but its direct support is currently limited to one ABCH system and should not be generalized to all ABCH orthologs or to ABCG transporters without comparative validation. We distinguish three experimentally separable interaction modes: competitive occupancy, spatial blockade, and functional uncoupling. Because direct proof that endogenous lipids and insecticides compete within the same transporter cycle remains limited, we propose an evidence-tier framework that integrates structural, biochemical, physiological, and genetic validation. Finally, we discuss how RNA interference, CRISPR/Cas9, and structure-guided perturbation can support functional testing while remaining constrained by delivery, specificity, field feasibility, and non-target arthropod safety.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"222 ","pages":"Article 107255"},"PeriodicalIF":4.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148579765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “PxTret1-mediated trehalose transport regulates development and reproduction in Plutella xylostella with implications for Cry1Ac tolerance” [Pesticide Biochemistry and Physiology 222 (2026) 107221]","authors":"Yingpeng Wang,&nbsp;Qiaoru Fu,&nbsp;Liya Xie,&nbsp;Peipei Wei,&nbsp;Ayipiri Refukaiti,&nbsp;Bailin Xia,&nbsp;Qisheng Song,&nbsp;Miao Xie","doi":"10.1016/j.pestbp.2026.107241","DOIUrl":"10.1016/j.pestbp.2026.107241","url":null,"abstract":"","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"222 ","pages":"Article 107241"},"PeriodicalIF":4.8,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148579784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and physiological evidence for magnolol antifungal activity against fusarium oxysporum f. sp. cubense tropical race 4","authors":"Wei Zhang ,&nbsp;Yushan Liu ,&nbsp;Mudassar Ahmad ,&nbsp;Huoqing Huang ,&nbsp;Siwen Liu ,&nbsp;Dandan Xiang ,&nbsp;Yong Liu ,&nbsp;Huinan Huang ,&nbsp;Chunyu Li ,&nbsp;Yadong Li","doi":"10.1016/j.pestbp.2026.107175","DOIUrl":"10.1016/j.pestbp.2026.107175","url":null,"abstract":"<div><div>Banana Fusarium wilt, caused by <em>Fusarium oxysporum</em> f. sp. <em>cubense</em> Tropical Race 4 (<em>Foc</em> TR4), ranks among the most devastating soil-borne fungal diseases. Prolonged use of single-target fungicides can lead to environmental contamination, resistance evolution, and limited efficacy due to the narrow range of target sites. Therefore, discovering an environmentally friendly fungicide with a novel mechanism of action and multiple targets is crucial for achieving sustainable disease management of banana Fusarium wilt. In this study, six plant-derived compounds were screened for antifungal activity, identifying magnolol (MAG) as the most effective inhibitor, with an EC₅₀ value of 18.17 μg·mL⁻¹. MAG significantly suppressed mycelial growth and conidial germination in vitro and markedly reduced disease incidence and severity in banana seedlings. Integrated transcriptomic and physiological analyses revealed that MAG disrupts membrane integrity, redox homeostasis, and energy metabolism, accompanied by suppression of RNA processing and ribosome biogenesis. Ultrastructural damage, membrane depolarization, and the study of antioxidant enzyme activity further confirmed the severe membrane disruption and the induction of ROS accumulation. Moreover, MAG exhibited broad-spectrum antifungal activity against multiple <em>Fusarium oxysporum</em> formae speciales and <em>Foc</em> races. Collectively, these findings highlight the multi-target antifungal mechanism of MAG, positioning it as a promising and eco-friendly candidate for controlling banana Fusarium wilt, offering a sustainable alternative to conventional chemical treatments. The broad-spectrum activity and low toxicity of MAG make it a potentially key component in future disease management strategies, particularly in reducing pesticide dependence and environmental pollution.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"221 ","pages":"Article 107175"},"PeriodicalIF":4.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148168035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of CYP51A, CYP51B, and CYP51C in the growth, reproduction, pathogenicity, and fungicide sensitivity of Fusarium oxysporum","authors":"Zhenyu Rong ,&nbsp;Shuodan Hu ,&nbsp;Chuanqing Zhang","doi":"10.1016/j.pestbp.2026.107174","DOIUrl":"10.1016/j.pestbp.2026.107174","url":null,"abstract":"<div><div><em>Fusarium oxysporum</em>, a pathogenic fungus distributed worldwide, causes destructive diseases, such as plant wilt, crown rot, root rot, and fruit rot. Sterol demethylation inhibitors (DMIs), which are the most widely used fungicides, inhibit cytochrome P450 sterol 14α-demethylase (<em>CYP51</em>) during sterol biosynthesis. <em>Fusarium</em> species contain three homologous <em>CYP51</em> genes, <em>FoCYP51A</em>, <em>FoCYP51B</em>, and <em>FoCYP51C</em>. Previous studies have shown that the deletion of individual <em>CYP51</em> genes does not significantly affect phenotype-related indicators, such as growth rate and sporulation, in <em>Fusarium</em> species, such as <em>F</em>. <em>graminearum</em>, <em>F</em>. <em>fujikuroi</em>, and <em>F</em>. <em>verticillioides</em>. This study aimed to determine the effects of <em>CYP51</em> genes on the growth, reproduction, pathogenicity, and DMI susceptibility of <em>F. oxysporum.</em> Unlike other <em>Fusarium</em> species, deletion of <em>CYP51A</em> reduced sporulation, germination, and pathogenicity, exacerbated cell membrane damage, and increased the DMI susceptibility in <em>F</em>. <em>oxysporum</em>. When a single <em>CYP51</em> gene was knocked out, other <em>CYP51</em> compensated by upregulating their expression to maintain balance. <em>CYP51A</em> could play a central role in this regulatory network. The absence of <em>CYP51C</em> did not cause noticeable changes in <em>F</em>. <em>oxysporum</em>. These results reveal the functional differentiation of <em>FoCYP51</em> homologs in regulating growth, pathogenicity, and DMI susceptibility and provide an understanding of the pathogenic mechanisms and fungicide responses of <em>F</em>. <em>oxysporum</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"221 ","pages":"Article 107174"},"PeriodicalIF":4.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148167983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the immunotoxic link between deltamethrin and coronary heart disease through network toxicology, molecular simulations and in vitro validation","authors":"Zheng Zhao ,&nbsp;Hongtao Lan ,&nbsp;Yanan Liang ,&nbsp;Tiantian Shan ,&nbsp;Jiatong Wu ,&nbsp;Changan Qu ,&nbsp;Sijia Liu ,&nbsp;Xianming Chu","doi":"10.1016/j.pestbp.2026.107161","DOIUrl":"10.1016/j.pestbp.2026.107161","url":null,"abstract":"<div><div>Deltamethrin is a commonly applied pyrethroid insecticide; however, its relationship with the risk of coronary heart disease (CHD) remains unclear. Given its widespread residues in the environment and food, and the growing concern over its long-term cardiovascular effects, it is of public health importance to determine whether and how deltamethrin exposure contributes to CHD. This study integrated data from the Global Burden of Disease study (GBD 2021) and Mendelian randomization (MR) to assess causality. Utilizing transcriptomic data associated with CHD and the predicted targets of deltamethrin, core genes were identified through weighted gene co-expression network analysis (WGCNA), protein-protein interaction (PPI) network analysis, and machine learning, pinpointing SPI1, IL1B, ICAM1 and FCER1G. These genes were enriched in immune-inflammatory pathways, associated with a pro-inflammatory microenvironment, and showed specific expression in monocytes. Molecular docking and dynamics simulations confirmed stable binding of deltamethrin to these targets. In vitro experiments demonstrated that deltamethrin dose-dependently inhibited cell viability, induced oxidative stress, and upregulated the expression of the core genes. In conclusion, deltamethrin may increase the risk of CHD, possibly by disrupting the immune-inflammatory networks involving SPI1, IL1B, ICAM1 and FCER1G, providing novel insights into the cardiovascular toxic effects of pyrethroids.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"221 ","pages":"Article 107161"},"PeriodicalIF":4.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148168036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}