Molecular characterization of Spodoptera frugiperda nose resistant to fluoxetine protein 6 and its putative involvement in tolerance to cyantraniliprole
Jiaping Wei , Changpeng Liu , Daojie Guan , Zhichao Wang , Huichen Ge , Hai Li , Hainan Zhang , Kun Qian , Jianjun Wang
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引用次数: 0
Abstract
Spodoptera frugiperda (FAW) is a notorious polyphagous pest that has developed resistance to various insecticides including diamide insecticides. Our previous study established a FAW cyantraniliprole-resistant (SfCYAN-R) strain by laboratory resistance selection of susceptible strain (SfCYAN-S), however, the potential resistance mechanisms of FAW to cyantraniliprole remain unclear. In this study, SfNrf6 encoding nose resistant to fluoxetine (Nrf) protein 6 was identified to be upregulated in SfCYAN-R strain compared with SfCYAN-S strain based on RNA-Seq data and RT-qPCR. The cDNA of Nrf6 was 2094 base pairs in length and encoded a protein of 697 amino acids. Sequence analysis revealed that SfNrf6 contained the conserved Nrf domain as well as acyltransferase domain, and shared high amino acid identity with Bombyx mori homologue. RT-qPCR analysis revealed that SfNrf6 was highly expressed in the midgut, and was upregulated at 1 h and 2 h after exposure of the third instar larvae to cyantraniliprole. Notably, knockdown of SfNrf6 significantly increased the susceptibility of FAW to cyantraniliprole. Furthermore, cytotoxicity assay demonstrated that overexpression of SfNrf6 enhanced Sf9 cell viability under cyantraniliprole treatment. These results provided preliminary evidence that SfNrf6 was involved in cyantraniliprole tolerance in FAW, which had applied implications for FAW resistance management.
期刊介绍:
Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance.
Research Areas Emphasized Include the Biochemistry and Physiology of:
• Comparative toxicity
• Mode of action
• Pathophysiology
• Plant growth regulators
• Resistance
• Other effects of pesticides on both parasites and hosts.