Pesticide Biochemistry and Physiology最新文献_第2页

Reverse engineering high-level resistance to Bt Cry1Ac toxin in Plutella xylostella reveals a hormonal regulatory feedback pathway

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-22 DOI: 10.1016/j.pestbp.2025.106382

Dan Sun , Mingyun Wang , Le Guo , Xuping Shentu , Xiaoping Yu , Neil Crickmore , Xuguo Zhou , Youjun Zhang , Zhaojiang Guo

{"title":"Reverse engineering high-level resistance to Bt Cry1Ac toxin in Plutella xylostella reveals a hormonal regulatory feedback pathway","authors":"Dan Sun , Mingyun Wang , Le Guo , Xuping Shentu , Xiaoping Yu , Neil Crickmore , Xuguo Zhou , Youjun Zhang , Zhaojiang Guo","doi":"10.1016/j.pestbp.2025.106382","DOIUrl":"10.1016/j.pestbp.2025.106382","url":null,"abstract":"<div><div>Decoding the molecular mechanisms of insect resistance to <em>Bacillus thuringiensis</em> (Bt) toxins is crucial for the sustainable utilization of Bt-based bioinsecticides and transgenic crops. Our previous studies showed that a hormone-responsive transcription factor FOXO binds to an inserted short interspersed nuclear element (SINE, named SE2), causing <em>MAP4K4</em> overexpression and resistance to Bt Cry1Ac toxin in <em>Plutella xylostella</em>. Furthermore, titers of two upstream signaling hormones (20-hydroxyecdysone and juvenile hormone) were also found to be elevated in the resistant strain, but it was unclear whether this was due to natural variation or a feedback pathway. Here, we established a homozygous knock-in strain (SE2-KI) using a reverse genetic approach to insert the SE2 retrotransposon into the <em>MAP4K4</em> promoter of a Cry1Ac-susceptible strain. The SE2 insertion induced <em>MAP4K4</em> overexpression, which in turn caused a downregulation of midgut receptors and an identical resistance phenotype to that seen in the evolved resistant strain. Moreover, SE2 insertion significantly increased the levels of two insect hormones providing definitive evidence for a positive feedback regulatory pathway. This study unveils an as yet uncharacterized hormonal regulatory feedback pathway orchestrating Cry1Ac resistance in <em>P. xylostella</em>, providing new insights into the molecular basis of Bt resistance and informing suitable field resistance management strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106382"},"PeriodicalIF":4.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytochrome CYP72A15 may play a role in metabolic resistance to mesotrione in wild radish

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-19 DOI: 10.1016/j.pestbp.2025.106380

Feng-Yan Zhou , Huan Lu , Xueping Huang , Yunjing Han , Yong Zhang , Heping Han , Liudmyla Tsykalchuk , Alex Nyporko , Qin Yu

{"title":"Cytochrome CYP72A15 may play a role in metabolic resistance to mesotrione in wild radish","authors":"Feng-Yan Zhou , Huan Lu , Xueping Huang , Yunjing Han , Yong Zhang , Heping Han , Liudmyla Tsykalchuk , Alex Nyporko , Qin Yu","doi":"10.1016/j.pestbp.2025.106380","DOIUrl":"10.1016/j.pestbp.2025.106380","url":null,"abstract":"<div><div>In our previous study with a wild radish (<em>Raphanus raphanistrum)</em> population resistant to HPPD-inhibiting herbicides we indicated that two additional candidate P450 genes, <em>CYP71A28</em> and <em>CYP72A13</em>-like, may also contribute to the resistance. This study investigates the role of these two P450 genes in mesotrione resistance in the wild radish population. The full-length <em>R. raphanistrum</em> P450 genes (thereafter named as <em>RrCYP71A28</em> and <em>RrCYP72A15)</em> were cloned from mesotrione-resistant (R) and -susceptible (S) wild radish plants. RT-qPCR results showed that basal expression levels of the two P450 genes are significantly higher (up to 3-fold) in the R than the S plants. <em>Escherichia coli</em> cells transformed respectively with <em>RrCYP71A28</em> and <em>RrCYP72A15</em> were more tolerant to mesotrione. Transgenic Arabidopsis plants expressing <em>RrCYP72A15</em> showed a modest level of resistance to mesotrione. UPLC-MS/MS analysis demonstrated that tissue mesotrione levels in <em>RrCYP72A15</em> transgenic Arabidopsis plants were significantly lower (up to 2-fold) than that in the wild type. Structural modelling predicts CYP72A15 can bind to RrCYP72A15 and metabolize mesotrione likely through formation of 4-OH-mesotrione. Although the <em>RrCYP72A15</em> gene confers a modest level of resistance, overexpression of the multiple herbicide-metabolizing genes could contribute to the low level of mesotrione resistance observed in the R wild radish population.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106380"},"PeriodicalIF":4.2,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the mode of action of BtEcR/USP-LBD with benzpyrimoxan in combination with high throughput SPR screening and molecular simulation approaches

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-18 DOI: 10.1016/j.pestbp.2025.106384

Hongyan Wang , Jialin Cui , Yanjiao Feng , Xinpeng Sun , Qinyan Tan , Li Zhang

{"title":"Understanding the mode of action of BtEcR/USP-LBD with benzpyrimoxan in combination with high throughput SPR screening and molecular simulation approaches","authors":"Hongyan Wang , Jialin Cui , Yanjiao Feng , Xinpeng Sun , Qinyan Tan , Li Zhang","doi":"10.1016/j.pestbp.2025.106384","DOIUrl":"10.1016/j.pestbp.2025.106384","url":null,"abstract":"<div><div><em>Bemisia tabaci</em> (<em>B. tabaci</em>) is a major agricultural pest that infests over 500 plant species, posing a significant threat to agricultural production due to its polyphagy, adaptability, and ability to transmit plant viruses. Excessive and improper insecticide use has caused resistance to pyrethroids, organophosphates, and neonicotinoids, creating an urgent need for new insecticides with novel structures and mechanisms of action. In this study, we developed an <em>in vitro</em> test platform targeting <em>B. tabaci</em> ecdysteroid receptor (<em>Bt</em>EcR/USP-LBD) using Surface Plasmon Resonance (SPR) and investigated the novel insect growth regulator benzpyrimoxan through SPR, molecular docking, and molecular dynamics (MD) simulations. Benzpyrimoxan specifically bound to <em>Bt</em>EcR/USP-LBD with a kinetic K<sub>D</sub> of 14.19 μM, but its binding strength was lower than that of PonA (K<sub>D</sub> = 0.21 μM). SPR and MD analyses showed that benzpyrimoxan had a slower binding rate and weaker interactions with Cys394 and Asn390 in the ligand binding domain of <em>Bt</em>EcR (<em>Bt</em>EcR-LBD), compared to PonA. Met389, Asn390, Thr393 and Cys394 have been shown to establish a specific hydrogen-bonding network in <em>Bt</em>EcR-LBD, which exhibits significant variations in <em>Hv</em>EcR-LBD. Molecular docking and MD simulations showed that benzpyrimoxan forms hydrogen bonds with this network but requires greater stability to enhance binding. This study identifies the potential mode of action of benzpyrimoxan and offers a strategy for discovering novel ecdysteroid analogues for controlling <em>B. tabaci</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106384"},"PeriodicalIF":4.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNAi effect in target and non-target pests correlates with the length of continuous matches in dsRNA sequences

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-17 DOI: 10.1016/j.pestbp.2025.106381

Zhaoyang Li , Junna Liu , Satyabrata Nanda , Zexin Zhong , Xuming Luo , Xuguo Zhou , Youjun Zhang , Chunxiao Yang , Huipeng Pan

{"title":"RNAi effect in target and non-target pests correlates with the length of continuous matches in dsRNA sequences","authors":"Zhaoyang Li , Junna Liu , Satyabrata Nanda , Zexin Zhong , Xuming Luo , Xuguo Zhou , Youjun Zhang , Chunxiao Yang , Huipeng Pan","doi":"10.1016/j.pestbp.2025.106381","DOIUrl":"10.1016/j.pestbp.2025.106381","url":null,"abstract":"<div><div>RNA interference (RNAi) has emerged as a promising and environmentally friendly approach for controlling the pest <em>Henosepilachna vigintioctopunctata</em> (Hvig). Identifying lethal target genes in Hvig and evaluating the efficacy of oral dsRNA administration are crucial steps in this process. Additionally, assessing the potential risks of RNAi to non-target organisms (NTOs) is essential to ensure environmental safety. A soluble N-ethylmaleimide-sensitive factor attachment protein α (αSNAP) is an essential component of membrane fusion machinery, offering as a potential target gene for RNAi-based pest control. This study found that silencing of <em>Hvαsnap</em> with varying dsRNA concentrations (6.25, 12.5, 25, 50, 100, 200 ng/μL) induced 53.33 %–100 % lethality in Hvig, with an LC<sub>50</sub> value of 10.15 ng/μL. Feeding the NTO, <em>Propylaea japonica</em> with ds<em>Hvαsnap</em> or ds<em>Pjαsnap-1</em> containing 3–21-nt consecutive matches had no notable effects on survival, development, pupal weight, or gene expression. However, injecting these dsRNAs significantly increased <em>P. japonica</em> mortality. A chimeric ds<em>GFP-αsnap-17-nt</em> suppressed <em>Hvαsnap</em> expression and reduced Hvig larval survival but failed to induce RNAi in <em>P. japonica</em>. Overall, this study suggests that different species exhibit varying sensitivities to dsRNA, and increasing the number of consecutive matching bases may enhance RNAi effects in NTOs.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106381"},"PeriodicalIF":4.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CYP321F3 mediates metabolic resistance to methoxyfenozide in rice stem borer, Chilo suppressalis

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-15 DOI: 10.1016/j.pestbp.2025.106383

Fang-Rui Guo , Shu-Chao Wang , Yan Liu , Shuai Wang , Jing-Mei Huang , Hao Sun , Lin-Feng He , Yuan Xie , Song-Tao Qiao , Feng-Xia Yang , Chris Bass , Cong-Fen Gao , Shun-Fan Wu

{"title":"CYP321F3 mediates metabolic resistance to methoxyfenozide in rice stem borer, Chilo suppressalis","authors":"Fang-Rui Guo , Shu-Chao Wang , Yan Liu , Shuai Wang , Jing-Mei Huang , Hao Sun , Lin-Feng He , Yuan Xie , Song-Tao Qiao , Feng-Xia Yang , Chris Bass , Cong-Fen Gao , Shun-Fan Wu","doi":"10.1016/j.pestbp.2025.106383","DOIUrl":"10.1016/j.pestbp.2025.106383","url":null,"abstract":"<div><div>The development of insecticide resistance in insect populations is a major challenge to sustainable agriculture and food security worldwide. Methoxyfenozide, an insect growth regulator that acts as an agonist of 20-hydroxyecdysone (20E), has severely declined in its efficacy against the rice stem borer (<em>Chilo suppressalis</em>), a notorious pest of rice crops in East and Southeast Asia. To date, however, the genes involved in methoxyfenozide resistance in target pests remain unclear. We conducted a long-term (seven years from 2017 to 2023) and large geographical scale (8 provinces and 45 cities in China) resistance monitoring program for methoxyfenozide in <em>C. suppressalis</em>. Resistance was seen to arise rapidly in this species, with >100-fold resistance being detected in nearly all the field populations after 2018<strong>.</strong> Piperonyl butoxide (PBO), an inhibitor of cytochrome P450 enzymes (P450s), significantly increased the sensitivity of resistant strains of <em>C. suppressalis</em> to methoxyfenozide, implicating P450s in resistance. Six P450 genes: <em>CYP321F3, CYP6CV5, CYP9A68</em>, <em>CYP6AB45, CYP324A12</em> and <em>CYP6SN2</em> were identified as highly expressed in resistant <em>C. suppressalis</em> by transcriptome profiling. Of these, ectopic expression of <em>CYP321F3</em> in <em>Drosophila melanogaster</em> resulted in a 7.0-fold increase in resistance to methoxyfenozide demonstrating its causal role in resistance. Collectively, these findings provide insight into the mechanisms mediating resistance to insect growth regulators and will inform the development of future pest and resistance management strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106383"},"PeriodicalIF":4.2,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of a novel cytochrome P450 CYP6HX3 from a specialist herbivore, Pagiophloeus tsushimanus, in the metabolism of host-plant terpenoids

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-11 DOI: 10.1016/j.pestbp.2025.106366

Shouyin Li , Jinyan Guo , Hui Li , Dejun Hao

{"title":"Involvement of a novel cytochrome P450 CYP6HX3 from a specialist herbivore, Pagiophloeus tsushimanus, in the metabolism of host-plant terpenoids","authors":"Shouyin Li , Jinyan Guo , Hui Li , Dejun Hao","doi":"10.1016/j.pestbp.2025.106366","DOIUrl":"10.1016/j.pestbp.2025.106366","url":null,"abstract":"<div><div>Cytochromes P450 have been confirmed to be involved in plant terpenoid biosynthesis and the degradation and metabolism of exogenous terpenoids in herbivorous organisms. Nevertheless, the underlying molecular mechanisms of P450-mediated terpenoid metabolism in numerous non-model insects remain largely unclear, which impedes our understanding of the chemical interactions between plants and insects. Herein, we identified a novel P450 gene belonging to CYP6 family, designated as <em>CYP6HX3</em>, from a specialist herbivore on camphor trees, <em>Pagiophloeus tsushimanus. CYP6HX3</em> transcripts were constitutively abundant in the gut and fat body of larvae, and its expression in various tissues (except for head) was significantly induced by specific terpenoids in camphor trees (D-camphor, linalool, and eucalyptol) to varying degrees. Additionally, the CYP6HX3 protein model was constructed accurately, and it could stably bind to the three terpenoid molecules mainly via hydrophobic forces. The capability of CYP6HX3 to metabolize the three terpenoids was verified using metabolic assays in vitro, and this monooxygenase catalyzed the epoxidation of linalool to (<em>R</em>/<em>S</em>)-furanoid-linalool oxide. These results will enhance our understanding of insect metabolic resistance to natural chemicals and offer new targets for pest management.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106366"},"PeriodicalIF":4.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Algicidal activity and mechanism of novel Bacillamide a derivative against red tide algae Skeletonema costatum and Prorocentrum minimum

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-11 DOI: 10.1016/j.pestbp.2025.106379

Huili Li , Xiaoxue Li , Yi Liu , Ronglian Xing , Hongxia Zhang , Wenguang Jia , Lihong Chen , Rui Li , Zhen Yu , Zhihong Tang

{"title":"Algicidal activity and mechanism of novel Bacillamide a derivative against red tide algae Skeletonema costatum and Prorocentrum minimum","authors":"Huili Li , Xiaoxue Li , Yi Liu , Ronglian Xing , Hongxia Zhang , Wenguang Jia , Lihong Chen , Rui Li , Zhen Yu , Zhihong Tang","doi":"10.1016/j.pestbp.2025.106379","DOIUrl":"10.1016/j.pestbp.2025.106379","url":null,"abstract":"<div><div>Frequent red tide outbreaks pose a serious threat to biodiversity and the safety of aquatic ecosystems. Bacillamides showed algicidal activity against algae. However, the low natural concentrations and their structural complexity hinder development of these molecules. Inspired by the natrual algicide Bacillamide A, a series of thiourea derivatives were synthesized. Bacillamide A derivative (<strong>3B</strong>) showed excellent algicidal activity against <em>S. costatum</em> (EC<sub>50</sub> = 0.52 μg/mL) and <em>P. minimum</em> (EC<sub>50</sub> = 2.99 μg/mL), respectively. In addition, it has low toxicity to mammals and is less toxic than copper sulfate. <strong>3B</strong> treatment resulted in loss of algal cell integrity. It also decreased the Chlorophyll a content and <em>Fv/fm</em> of algal cells, while increasing the levels of malondialdehyde content, superoxide dismutase, and reactive oxygen. 3B also induced expression of the photosynthetic genes, including <em>psaB</em>, <em>psbB</em>, as well as the antioxidant genes <em>SOD2</em> and <em>CAT</em>. This study demonstrates that Bacillamide A derivatives could provide a safer alternative for red tide algal management.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106379"},"PeriodicalIF":4.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization of chemosensory proteins in three sympatric Tomicus bark beetles feeding on Pinus yunnanensis: Implication for the conservation and divergence of ligand-binding profiles

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-09 DOI: 10.1016/j.pestbp.2025.106371

Lin-Mei Pu , Peng-Fei Wang , Yu-Yue Lu , An-Jin Yang , Li-Li Liu , Nai-Yong Liu

{"title":"Functional characterization of chemosensory proteins in three sympatric Tomicus bark beetles feeding on Pinus yunnanensis: Implication for the conservation and divergence of ligand-binding profiles","authors":"Lin-Mei Pu , Peng-Fei Wang , Yu-Yue Lu , An-Jin Yang , Li-Li Liu , Nai-Yong Liu","doi":"10.1016/j.pestbp.2025.106371","DOIUrl":"10.1016/j.pestbp.2025.106371","url":null,"abstract":"<div><div>Three sympatric <em>Tomicus</em> bark beetles, <em>Tomicus yunnanensis</em>, <em>Tomicus brevipilosus</em> and <em>Tomicus minor</em> that coexist in the trunks of a pine but occupy different spatiotemporal ecological niches, are the best-studied examples for addressing functional differentiation of chemosensory protein (CSP) orthologs. Here, totally 36 CSP-coding genes belonging to 12 groups of orthologs were identified from three <em>Tomicus</em> beetles. <em>Tomicus</em> CSP orthologs shared the high conservation and slight differences in sequence characteristics, phylogenetic relationships and tissue expression profiles. Binding assays revealed that 12 antenna-enriched <em>Tomicus</em> CSPs could respond strongly to non-host volatiles, pheromones and insecticides where each group of CSP orthologs presented diverse ligand-binding properties. CSP1 and CSP2 could interact strongly with benzaldehyde and salicylaldehyde (dissociation constant, K<sub>i</sub> < 14 μM) whereas CSP10 and CSP11 preferred to bind 1-hexanol and acetophenone (K<sub>i</sub> < 13 μM). All the proteins were tuned to chlorpyrifos with particularly high affinities (K<sub>i</sub> < 10 μM). Two conserved residues (glutamine, Q64 and leucine, L72) of CSP11 orthologs contributed to the specific binding to optimal ligands, but exhibited opposite binding properties between chlorpyrifos and other four compounds. Our study sheds light on the functional conservation and divergence of <em>Tomicus</em> CSP orthologs in olfactory reception and insecticide resistance, implying the correlation between ecological niche differentiation and olfactory specialization in three <em>Tomicus</em> bar beetles.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106371"},"PeriodicalIF":4.2,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143593960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sublethal indoxacarb exposure alters pheromone production and ovarian development in the yellow peach moth, Conogethes punctiferalis

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-09 DOI: 10.1016/j.pestbp.2025.106368

Yunhui Zhang , Yao Zhang , Zhen Li , Xiaoguang Liu , Xiaoming Liu , Shuangyan Yao , Mengfang Du , Shiheng An

{"title":"Sublethal indoxacarb exposure alters pheromone production and ovarian development in the yellow peach moth, Conogethes punctiferalis","authors":"Yunhui Zhang , Yao Zhang , Zhen Li , Xiaoguang Liu , Xiaoming Liu , Shuangyan Yao , Mengfang Du , Shiheng An","doi":"10.1016/j.pestbp.2025.106368","DOIUrl":"10.1016/j.pestbp.2025.106368","url":null,"abstract":"<div><div>The <em>Conogethes punctiferalis</em>, a major pest of peach and other fruit trees, causes significant damage by boring into fruits during its larval stage. Indoxacarb, a sodium channel blocker insecticide, has been widely applied in agriculture and horticulture for pest control, particularly against larval pests. However, its effects on adult insects remain largely unexplored. Present study employed <em>C. punctiferalis</em> as model to investigate the toxic effects of indoxacarb on adult moths. The results showed that sublethal exposure to indoxacarb significantly reduced the release of sex pheromones, reduced female attraction to males, and lowered mating success rate. Further investigations indicated that exposure to sublethal indoxacarb resulted in a significant decrease in Ca<sup>2+</sup> levels in the pheromone gland (PG), subsequently affecting the activities of calcineurin and acetyl-CoA carboxylase as well as affecting the expression levels of genes related to sex pheromone biosynthesis. Physiological assays revealed that indoxacarb exposure significantly reduced trehalose content, hexokinase activity, and pyruvic acid content in the PG. Moreover, ovarian development was hindered as the exposure led to reduced ovarian size and vitellogenin (Vg) content. Transcriptomic analysis revealed change in genes linked to ovarian development, including <em>Vg</em>, vitellogenin receptor (<em>VgR</em>), and genes related to lipid metabolism. In conclusion, this study demonstrates that indoxacarb exerts a dual regulatory effect on adult <em>C. punctiferalis</em>, inhibiting both sex pheromone biosynthesis and ovarian development. These findings provide novel insights into the mechanisms by which sublethal pesticide exposure influences adult moths.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106368"},"PeriodicalIF":4.2,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spinosyn resistance and cross-resistance – A 25 year review and analysis

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-08 DOI: 10.1016/j.pestbp.2025.106363

Thomas C. Sparks , Frank J. Wessels , Trent Perry , Michael J. Price , Melissa W. Siebert , David G.J. Mann

{"title":"Spinosyn resistance and cross-resistance – A 25 year review and analysis","authors":"Thomas C. Sparks , Frank J. Wessels , Trent Perry , Michael J. Price , Melissa W. Siebert , David G.J. Mann","doi":"10.1016/j.pestbp.2025.106363","DOIUrl":"10.1016/j.pestbp.2025.106363","url":null,"abstract":"<div><div>The spinosyns were introduced more than 25 years ago (spinosad - Qalcova™ active, 1997) and later followed by spinetoram (Jemvelva™ active, 2007), for the control of a wide range of pest insects, especially lepidopterans and thrips. Although IRM guidelines were initiated at the time of introduction, there have been some cases of overuse, resulting in resistance development in several pest insect species. Additionally, laboratory selection for spinosyn resistance has been a tool to investigate the mode of action of the spinosyns. As part of an ongoing effort to understand the scope of resistance to the spinosyns, >400 studies covering >1100 cases were analyzed where resistance to the spinosyns was examined (field or laboratory studies) or spinosyns were examined for cross-resistance to other insecticides. To date there have been 400 cases where spinosyn-selected resistance was investigated, with half in field strains of which only a few were associated with sub-optimal control. Most cases of field selected spinosyn resistance involved thrips (46 %), lepidopterans (22 %) or dipterans (18 %). Importantly, only a small percentage of the field selected spinosyn resistance cases show high levels of resistance (>1000-fold), with many (∼30 %) exhibiting either no or limited levels of resistance. There was no correlation in spinosyn-selected insects for cross-resistance to other insecticide classes (e.g. neonicotinoids, avermectins diamides, pyrethroids). Analysis of 400+ studies indicates that cross-resistance to the spinosyns from other insecticides is also unlikely, potentially driven by the unique chemistry and novel mode of action of the spinosyns. Thus, the overall impact of spinosyn resistance is far less than might be implied from the large and expanding body of studies over the last 25 years.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106363"},"PeriodicalIF":4.2,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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