Pesticide Biochemistry and Physiology最新文献

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Comprehensive effects of acetamiprid uptake and translocation from soil on pak choi and lettuce at the environmental level 啶虫脒从土壤中吸收和转移对白菜和莴苣环境水平的综合影响
IF 4.2 1区 农林科学
Pesticide Biochemistry and Physiology Pub Date : 2024-10-18 DOI: 10.1016/j.pestbp.2024.106178
{"title":"Comprehensive effects of acetamiprid uptake and translocation from soil on pak choi and lettuce at the environmental level","authors":"","doi":"10.1016/j.pestbp.2024.106178","DOIUrl":"10.1016/j.pestbp.2024.106178","url":null,"abstract":"<div><div>Acetamiprid (ACE) is widely used in agriculture to control pests. However, its accumulation in soil and subsequent translocation to plants can impact plant growth and development through mechanisms that remain unclear. This study evaluated the comprehensive effects of residual ACE from soil on cultivated pak choi and lettuce at environmental levels. Results showed that more than 90 % of ACE residues in the soils dissipated within 14 days. The average root concentration factor (RCF) values of pak choi and lettuce were 1.442 and 0.318, respectively, while the average translocation factor (TF) values were 2.145 for pak choi and 5.346 for lettuce. Seedling height increased by 6.32 % in pak choi but decreased by 8.54 % in lettuce. Furthermore, chlorophyll content decreased by 14.6 % in pak choi and increased by 23.7 % in lettuce. Non-targeted metabolomics analysis showed significant disturbances in carbohydrates, amino acids, and secondary metabolite levels. Additionally, KEGG pathway analysis revealed the down-regulation of amino acid metabolites in both vegetables, alongside an up-regulation of flavone and flavonol biosynthesis in pak choi. This research enhances the understanding of the effects and underlying metabolic mechanism of ACE on different vegetables.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dimethyl trisulfide reduces postharvest anthracnose and enhances mango quality, and a potential molecular mechanism against Colletotrichum gloeosporioides 二甲基三硫醚可减少采后炭疽病并提高芒果品质,是防治球孢子菌的潜在分子机制
IF 4.2 1区 农林科学
Pesticide Biochemistry and Physiology Pub Date : 2024-10-18 DOI: 10.1016/j.pestbp.2024.106174
{"title":"Dimethyl trisulfide reduces postharvest anthracnose and enhances mango quality, and a potential molecular mechanism against Colletotrichum gloeosporioides","authors":"","doi":"10.1016/j.pestbp.2024.106174","DOIUrl":"10.1016/j.pestbp.2024.106174","url":null,"abstract":"<div><div>Mango anthracnose, mainly caused by <em>Colletotrichum gloeosporioides</em>, is the major destructive postharvest disease of mango during storage and transport. Dimethyl trisulfide (DMTS), an organic volatile found in some microorganisms or plants, inhibited growth of <em>C. gloeosporioides in vitro</em>, but its effects on mango anthracnose and its molecular mechanisms of action have not been well characterized. In this study, the EC<sub>50</sub> of DMTS against <em>Colletotrichum spp.</em> from mango mainly ranged from 2.3 to 20.0 μL/L. <em>In vivo</em>, the fumigation rates of 20 μL/L of DMTS for 24 h, or 80 μL/L for 3 h or 6 h could effectively reduce severity of anthracnose (natural inoculum) on postharvest mangoes with inhibitory effects of 61.7 %, 65.7 %, and 69.4 %, respectively, as observed 10 days after treatment. Furthermore, there was no detectable DMTS residue in mango skin or flesh, and an overall improvement in the quality of the fruit with higher soluble solids, total sugars, vitamin c, and β-carotene, and lower titratable acidity than the non-treated control. In addition, DMTS could significantly reduce ergosterol content in mycelia of <em>C. gloeosporioides</em>, and gene expression analysis showed DMTS significantly suppressed expression of ergosterol biosynthesis-related genes <em>Cgerg6</em> and <em>Cgerg11</em> after mycelia were exposed to DMTS. Knock-out mutants for each of these two genes showed reduced sensitivity to DMTS. After gene complementation <em>in situ</em>, the sensitivity of complementary transformants to DMTS was restored to that of the parental strain. Therefore, we concluded that the genes <em>Cgerg6</em> and <em>Cgerg11</em> are involved in an interaction with the antifungal activity of DMTS. This is the first study to demonstrate a control effect of DMTS on mango postharvest anthracnose resulting in reduced disease severity and enhanced fruit quality. Transformant studies also revealed some potential molecular mechanisms of the antifungal activity of DMTS that may lead to improved management of mango postharvest anthracnose.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Area-wide survey and monitoring of insecticide resistance in the brown planthopper, Nilaparvata lugens (Stål), from 2020 to 2023 in China 2020-2023年中国褐飞虱抗药性的全区调查与监测
IF 4.2 1区 农林科学
Pesticide Biochemistry and Physiology Pub Date : 2024-10-16 DOI: 10.1016/j.pestbp.2024.106173
{"title":"Area-wide survey and monitoring of insecticide resistance in the brown planthopper, Nilaparvata lugens (Stål), from 2020 to 2023 in China","authors":"","doi":"10.1016/j.pestbp.2024.106173","DOIUrl":"10.1016/j.pestbp.2024.106173","url":null,"abstract":"<div><div>The brown planthopper (BPH), <em>Nilaparvata lugens</em> (Stål), is a notorious pest affecting Asian rice crops. The evolution of insecticide resistance in BPH has emerged as a significant challenge in effectively managing this pest. This study revealed the resistance status of BPH to nine insecticides in ten provinces and Shanghai City in China from 2020 to 2023. Monitoring results showed that the resistance of BPH to triflumezopyrim, nitenpyram, and dinotefuran increased rapidly. The average resistance ratio of BPH to triflumezopyrim increased from 2.5 to 7.1 fold, nitenpyram from 18.3 to 37.7 fold, and dinotefuran from 119.5 to 268.1 fold. All populations remained extremely high resistance to imidacloprid, thiamethoxam, and buprofezin. Most field populations of BPH maintained moderate resistance to chlorpyrifos and sulfoxaflor, and high resistance to pymetrozine by rice stem dipping method. However, considering the reproduction-inhibiting character of pymetrozine, susceptible to low resistance levels to pymetrozine were monitored by Insecticide Resistance Action Committee (IRAC) NO.005 method. This result indicated that pymetrozine might lose efficacy in the control of application generation, but it could significantly inhibit the reproduction of field populations of BPH. Additionally, we compared the expression levels of 11 nicotinic acetylcholine receptor (nAChR) genes, the targets of nAChR competitive modulators, in four field populations (FY23, YH23, LJ23, LP23) and susceptible strain. The expression level of nAChR α4 was significantly reduced in all field populations, while α1, α2, α6, and α7 were significantly reduced in some field populations. Our findings provide valuable information for resistance management strategies in <em>N. lugens</em> and offer new insights into the resistance mechanisms of nAChR competitive modulators.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Resmethrin disrupts mitochondria-associated membranes and activates endoplasmic reticulum stress, leading to proliferation inhibition in cultured mouse Leydig and Sertoli cells 氯氰菊酯破坏线粒体相关膜,激活内质网应激,导致培养的小鼠莱迪格细胞和肥大细胞增殖受抑制
IF 4.2 1区 农林科学
Pesticide Biochemistry and Physiology Pub Date : 2024-10-16 DOI: 10.1016/j.pestbp.2024.106175
{"title":"Resmethrin disrupts mitochondria-associated membranes and activates endoplasmic reticulum stress, leading to proliferation inhibition in cultured mouse Leydig and Sertoli cells","authors":"","doi":"10.1016/j.pestbp.2024.106175","DOIUrl":"10.1016/j.pestbp.2024.106175","url":null,"abstract":"<div><div>Resmethrin, a pyrethroid pesticide used to control insects, is toxic to non-target organisms and other mammals. However, little is known about the reproductive toxicity of resmethrin in the testes, or its mechanism of toxicity. In this study, we investigated the testicular toxicity of resmethrin on mouse Leydig (TM3) and Sertoli (TM4) cells, focusing on the mitochondria and endoplasmic reticulum (ER). We found that resmethrin inhibited proliferation and cell cycle progression and disrupted mitochondrial membrane potential (MMP; ΔΨ) in TM3 and TM4 cells. In particular, resmethrin exposure significantly reduced the expression of mitochondria-associated membranes (MAMs) proteins, such as Vapb, Vdac, and Grp75, in both cell lines. Resmethrin also disrupts calcium homeostasis in the mitochondrial matrix and cytoplasm. In addition, resmethrin activates oxidative stress-mediated ER stress signals. Finally, we confirmed that 4-PBA, an ER stress inhibitor, restored the growth of TM3 and TM4 cells, which was decreased by resmethrin. Therefore, we confirmed that resmethrin hampered MAMs and activated ER stress, thus suppressing TM3 and TM4 cell proliferation.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Triazophos-induced spermotoxicity in rats: Protective effects of nano-quercetin 三唑磷诱导的大鼠精子毒性:纳米槲皮素的保护作用
IF 4.2 1区 农林科学
Pesticide Biochemistry and Physiology Pub Date : 2024-10-16 DOI: 10.1016/j.pestbp.2024.106176
{"title":"Triazophos-induced spermotoxicity in rats: Protective effects of nano-quercetin","authors":"","doi":"10.1016/j.pestbp.2024.106176","DOIUrl":"10.1016/j.pestbp.2024.106176","url":null,"abstract":"<div><div>This study aimed to evaluate the spermotoxic potential of triazophos in rats and to check the possible shielding effect of quercetin and nano-quercetin against triazophos-induced toxicity. Rats in Group I were given olive oil as a vehicle. Group II and Group III received high-dose and low-dose triazophos, respectively. Oral administration of quercetin (Group IV) and nano-quercetin (Group VI) at a dose of 50 mg/kg body weight was given to two additional groups of animals. Two other high-dose triazophos groups were co-administered with quercetin (Group V) and nano-quercetin (Group VII).</div><div>Triazophos administration for 60 days in rats altered the structural and functional parameters of spermatozoa and brought about a decline in total sperm count, percentage of viable sperms, drop in sperm motility, and decrease in the number of sperms showing normal morphology. It also decreased the number of spermatozoa with intact acrosomes and HOST-positive spermatozoa. Further, triazophos increased the levels of reactive oxygen species and triggered apoptotic pathways in spermatozoa in a dose-dependent manner. It decreased daily sperm production and caused histomorphological aberrations in the epididymis and vas deferens. Co-administration of nano-quercetin with triazophos effectively counteracted sperm-related pathological changes. Nano-quercetin offered better protection over quercetin in ameliorating the triazophos-induced spermotoxicity in rats.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improvement of chlorpyrifos-induced cognitive impairment by mountain grape anthocyanins based on PI3K/Akt signaling pathway 基于 PI3K/Akt 信号通路的山葡萄花青素对毒死蜱诱导的认知障碍的改善作用
IF 4.2 1区 农林科学
Pesticide Biochemistry and Physiology Pub Date : 2024-10-15 DOI: 10.1016/j.pestbp.2024.106172
{"title":"Improvement of chlorpyrifos-induced cognitive impairment by mountain grape anthocyanins based on PI3K/Akt signaling pathway","authors":"","doi":"10.1016/j.pestbp.2024.106172","DOIUrl":"10.1016/j.pestbp.2024.106172","url":null,"abstract":"<div><div>The organophosphorus insecticide Chlorpyrifos (CPF) is widely used worldwide due to its high effectiveness. However, when ingested through the mouth and nose, it can cause severe neurotoxic effects and cognitive impairment. Natural anthocyanins show great potential in improving cognitive impairment. In this paper, we will delve into the protective effect of anthocyanins on CPF-induced cognitive impairment and its mechanism through the PI3K/Akt signaling pathway. Morris water maze, histopathological, ELISA and western blot analyses showed that anthocyanins effectively ameliorated CPF-induced spatial learning memory impairment in mice by ameliorating CPF-induced AChE inhibition, oxidative stress, and neuroinflammation and by modulating the levels of apoptosis (Caspase-3, Caspase-9) and autophagy (LC3II/ LC3I, Beclin1, p62, mTOR) biomarkers, in order to restore damaged hippocampal tissue morphology, neuron and synapse structures. To identify the action pathway of anthocyanins, we used KEGG and GO pathway enrichment analysis for screening prediction and western blot and molecular docking to verify that anthocyanins improve CPF-induced cognitive impairment by activating the PI3K/Akt pathway.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quinclorac-resistant Echinochloa spp. promoted growth and reproduction of Laodelphax striatellus (Hemiptera: Delphacidae) probably by providing more nutrients and stable environment 抗喹喏拉克的越橘属植物可能通过提供更多的营养和稳定的环境促进了Laodelphax striatellus(半翅目:Delphacidae)的生长和繁殖
IF 4.2 1区 农林科学
Pesticide Biochemistry and Physiology Pub Date : 2024-10-13 DOI: 10.1016/j.pestbp.2024.106165
{"title":"Quinclorac-resistant Echinochloa spp. promoted growth and reproduction of Laodelphax striatellus (Hemiptera: Delphacidae) probably by providing more nutrients and stable environment","authors":"","doi":"10.1016/j.pestbp.2024.106165","DOIUrl":"10.1016/j.pestbp.2024.106165","url":null,"abstract":"<div><div>Rice is an important agricultural crop that faces serious challenges from pathogens, pests, and weeds during growth stages. Meanwhile, these organisms would interact with each other to increase the level of destruction. The previous studies showed that barnyard grass (<em>Echinochloa</em> spp) could be used as a temporary host to increase infestation of small brown planthopper (SBPH, <em>Laodelphax striatellus</em>), which is one of the main polyphagous pests. Herbicides are widely used to control weeds that induce resistance development. However, little is known about the effects of increased weed resistance on insect species. In this study, we investigated the effect of quinclorac-resistant and sensitive biotypes of barnyard grass (<em>Echinochloa crus-galli</em> var. <em>zelayensis</em>; <em>Echinochloa crus-pavonis</em> Schult) and rice plants (Wuyujing 3) on the ecological fitness of SBPH and examined physiological indicators of plants and SBPH to explore the mechanism. Our results showed that the growth and reproduction of SBPH promoted significantly reared on quinclorac-resistant barnyard grass. From the perspectives of oxidative stress response, the activities of peroxidase (POD) increased and the activities of catalase (CAT), mixed-functional oxidase (MFO), and carboxylesterase (CarE) decreased in SBPH reared on resistant barnyard grass. Combined with the increased amino acid contents (threonine, serine, methionine, and alanine) of resistant barnyard grass <em>E. crus-pavonis</em>, we speculate that quinclorac-resistant barnyard grass probably provides SBPH with a more suitable environment, thus increasing the risk of SBPH.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential expression of TOR complex 1 components in Colletotrichum camelliae isolates confers natural resistance to rapamycin 山茶科 Colletotrichum camelliae 分离物中 TOR 复合物 1 成分的差异表达赋予了对雷帕霉素的天然抗性
IF 4.2 1区 农林科学
Pesticide Biochemistry and Physiology Pub Date : 2024-10-10 DOI: 10.1016/j.pestbp.2024.106169
{"title":"Differential expression of TOR complex 1 components in Colletotrichum camelliae isolates confers natural resistance to rapamycin","authors":"","doi":"10.1016/j.pestbp.2024.106169","DOIUrl":"10.1016/j.pestbp.2024.106169","url":null,"abstract":"<div><div>Rapamycin is a lipophilic macrolide antibiotic which is famous for its immunosuppressive and anticancer activity. In recent years, rapamycin showed significant activity against various plant pathogenic fungi. However, the sensitivity of <em>Colletotrichunm</em> fungi to rapamycin is scarcely reported. In this study, we evaluated the sensitivity of 116 <em>Colletotrichum</em> isolates from tea-oil trees to rapamycin. Most isolates exhibited natural resistance with inhibition rates of 50 to 70% at 50 μg/mL. Three <em>Colletotrichum camelliae</em> isolates were found to be sensitive to rapamycin. No mutations were detected in the direct target FKBP12 and indirect target TOR-FRB domain of resistant and sensitive <em>C. camelliae</em> isolates. Notably, the expression of the TOR homolog (CcTOR) was higher in resistant <em>C. camelliae</em> isolates compared to the sensitive ones and overexpression of <em>CcTOR</em> in the sensitive isolate CcS1 resulted in decreased sensitivity to rapamycin. Moreover, ribosomal protein S6 phosphorylation was abolished in the sensitive isolate CcS1 but not in the resistant isolate CcR1 under rapamycin treatment. In addition, the expression levels of ribosome biogenesis genes and two other components of TORC1 were higher in CcR1 compared to CcS1 under the same treatment, which suggested that the abundance of TORC1 in CcR1 was greater than in CcS1, leading to more active TORC1 signaling in CcR1. These results provided a better understanding about natural resistance of <em>C. camelliae</em> isolates to rapamycin and could help for developing new TORC1 signaling-targeting fungicides.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142416765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptomics reveals the mechanism of terbuthylazine-induced nephrotoxicity in chickens: Insights from AMPK/p53-mediated apoptosis perspective 转录组学揭示特丁基嗪诱导鸡肾毒性的机制从 AMPK/p53 介导的细胞凋亡角度看问题
IF 4.2 1区 农林科学
Pesticide Biochemistry and Physiology Pub Date : 2024-10-10 DOI: 10.1016/j.pestbp.2024.106171
{"title":"Transcriptomics reveals the mechanism of terbuthylazine-induced nephrotoxicity in chickens: Insights from AMPK/p53-mediated apoptosis perspective","authors":"","doi":"10.1016/j.pestbp.2024.106171","DOIUrl":"10.1016/j.pestbp.2024.106171","url":null,"abstract":"<div><div>As a commonly used pesticide, the widespread use of terbuthylazine (TBA) may cause toxic effects in animals and human. However, the nephrotoxicity induced by TBA is unclear. Here, we explored the mechanism of TBA-induced nephrotoxicity through transcriptomics and molecular biology techniques in broilers. Pathologic analysis showed that TBA could cause renal cell vacuolation and fibrosis in broilers. Additionally, transcriptomic analysis showed that TBA can cause significant changes in the expression of some apoptosis-related genes, and GO and KEGG analysis also found that TBA can significantly change the functions of apoptosis pathway and AMPK signaling pathway in kidney. Subsequently, the protein expression levels of Bax, Bak-1, FADD, and cleaved Caspase-3/Caspase-3 were elevated significantly and the number of TUNEL-positive cells was increased markedly in kidney under TBA exposure. Meanwhile, we also found that TBA could activate AMPK/p53 pathway, as evidenced by the upregulated levels of AMPKα1 phosphorylation and protein expression of p53. Therefore, our results suggested that TBA could induce apoptosis via AMPK/p53 pathway in kidney. These findings identified the nephrotoxic mechanism of TBA through transcriptomics, providing a new insight into TBA toxicology.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential interactions of ethacrynic acid and diethyl maleate with glutathione S-transferases and their glutathione co-factor in the house fly 家蝇体内乙草胺和马来酸二乙酯与谷胱甘肽 S-转移酶及其谷胱甘肽辅助因子的不同相互作用
IF 4.2 1区 农林科学
Pesticide Biochemistry and Physiology Pub Date : 2024-10-10 DOI: 10.1016/j.pestbp.2024.106170
{"title":"Differential interactions of ethacrynic acid and diethyl maleate with glutathione S-transferases and their glutathione co-factor in the house fly","authors":"","doi":"10.1016/j.pestbp.2024.106170","DOIUrl":"10.1016/j.pestbp.2024.106170","url":null,"abstract":"<div><div>Glutathione <em>S</em>-transferases (GSTs) are an important class of enzymes that facilitate the conjugation of reduced glutathione (GSH) with electrophilic substrates, including some insecticides. Two inhibitors of GSTs, ethacrynic acid (EA) and diethyl maleate (DEM), are often used as diagnostic tools to implicate GST involvement in insecticide resistance, but their modes of action against insect GSTs are largely assumed based on mammalian studies. In mammalian studies, there are two proposed mechanisms of inhibition of GST function by EA and DEM: 1) scavenging or “depleting” cytosolic GSH through non-enzymatic conjugation, and 2) inhibition of GST activity directly by the inhibitor-GSH conjugate (EA-SG and DEM-SG).</div><div>The objective of this study was to characterize putative inhibitory mechanisms of EA and DEM against insect (house fly) GSTs and the co-factor GSH. Both EA and DEM synergized topical applications of naled and propoxur but not permethrin. As a GSH scavenger, EA was ∼10-fold more potent compared to DEM. Conditions such as pH, GSH concentration, and incubation time significantly affected the ability of both inhibitors to scavenge GSH. EA demonstrated scavenging at a wider pH range than DEM and scavenged GSH at a faster rate than DEM. Whereas EA peak scavenging was observed almost instantly, there was a 54.4 % increase in scavenged GSH for DEM between 0 and 30 min of incubation. Increasing concentration of GSH diminished the effect of scavenging at the highest tested concentrations of both inhibitors. In the presence of both GSH and GSTs in crude homogenate, EA was 300-fold more potent as a GST inhibitor compared to DEM at pH 7.5. No comparison was made at pH 6.5 because the tested concentrations of DEM did not produce enough inhibition to derive an IC<sub>50</sub> value while EA concentrations did. With purified GSTs, EA-SG was 205-fold more potent as an inhibitor compared to DEM-SG, while EA alone was 7.6-fold more potent than EA-SG and 1565-fold more potent than DEM-SG. These findings establish in insects that the insecticide synergists EA and DEM function mainly by scavenging the GST co-factor GSH, with some inhibition due to interactions with GSTs and the inhibitor-GSH conjugates, rather than through interaction between the inhibitors and the GST protein itself. These resulting impacts are two-fold, whereby (i) GSH bioavailability is limited and (ii) the GSH-inhibitor complex attenuates GST-based xenobiotic metabolism.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142440956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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