Pesticide Biochemistry and Physiology最新文献

{"title":"RNAi-mediated knockdown of juvenile hormone acid methyltransferase depresses reproductive performance in female Aethina tumida","authors":"Yifan Gu ,&nbsp;Xinyu Yang ,&nbsp;Senhao Liu ,&nbsp;Xiaowei Chen ,&nbsp;Ran Liu ,&nbsp;Jinglin Gao ,&nbsp;Yihai Zhong ,&nbsp;Xiaoyu Li ,&nbsp;Wensu Han","doi":"10.1016/j.pestbp.2025.106420","DOIUrl":"10.1016/j.pestbp.2025.106420","url":null,"abstract":"<div><div>Small hive beetles, <em>Aethina tumide</em>, are free-flying parasites of social bee colonies where they feed and reproduce. In case of mass infestation, <em>A. tumida</em> can cause significant economic losses. There is an urgent need to explore novel green molecular approaches for sustainable control of <em>A. tumida</em>. It has been confirmed that juvenile hormone acid methyl transferase (<em>JHAMT</em>) plays a crucial role in regulating the synthesis of juvenile hormone (JH). However, its impact on female reproduction of <em>A. tumida</em> remains unclear. In the present study, a novel <em>JHAMT</em> gene was identified from <em>A. tumida</em> with an open reading frame of 978 bp, encoding a polypeptide of 325 amino acids containing a Methyltransferase domain. The deduced amino acid sequence of AtJHAMT shared 60 % and 33 % identity with homologs from <em>Brassicogethes aeneus</em> and <em>Apis mellifera</em>, respectively. The expression profile indicates that the transcription level of <em>AtJHAMT</em> increases in the adult stages, reaching its peak in 5-day-old female adults. <em>AtJHAMT</em> exhibits the highest expression levels in the ovaries, and fluorescence in situ hybridization (FISH) demonstrates that this gene shows a significant number of positive signals in the ovarian ducts and the head region. Furthermore, we investigated the function of <em>AtJHAMT</em> through RNA interference and methoprene rescue experiments. We also investigeted the off-target effects of the <em>dsJHAMT</em>. The results showed that silencing <em>AtJHAMT</em> through oral dsRNA delivery (feeding dsRNA-SPc mix) affected ovarian development and significantly reduced JH titers, female fecundity, female fertility, and egg hatchability. The application of methoprene partially rescued the negative effect of silencing <em>AtJHAMT</em> on reproduction. Several genes associated with ovarian development were significantly downregulated following interference with <em>AtJHAMT</em>, but their expression levels were restored after complementation experiments. Additionally, the off-target effects experiment showed that <em>dsJHAMT</em> from <em>A. tumida</em> had no adverse effects on ovaries development in honey bee queens. Overall, this study illustrates the functions of the <em>JHAMT</em> in <em>A. tumida</em>, which can serve as a potential target for controlling the reproduction of the most deleterious bee parasites, <em>A. tumida</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106420"},"PeriodicalIF":4.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid surface droplet 2 (LSD2) regulates lipid metabolism and male reproductive physiology in adult Sitotroga cerealella","authors":"Sakhawat Shah ,&nbsp;Yu Li ,&nbsp;Wen-Han Yan ,&nbsp;Lianyun Liu ,&nbsp;Tianyu Gu ,&nbsp;Karam Khamis Elgizawy ,&nbsp;Gang Wu ,&nbsp;Feng-Lian Yang","doi":"10.1016/j.pestbp.2025.106417","DOIUrl":"10.1016/j.pestbp.2025.106417","url":null,"abstract":"<div><div>Insect metabolism plays a critical role in shaping reproductive physiology, offering valuable insight for pest management strategies and ecological understanding. In this study, we investigated the regulation of the lipid surface droplet protein <em>LSD2</em> and its impact on lipid metabolism and male reproductive events in <em>Sitotroga cerealella</em>. Using <em>ScLSD2</em> RNAi, we assessed it effects on energy metabolism, sperm count, motility, oviposition, and hatchability in <em>S. cerealella</em> adults. Our results reveal tissue-specific expression of <em>ScLSD2</em>, predominantly in the fat body. Silencing <em>ScLSD2</em> led to reduced mating rates, sperm motility parameters, oviposition, and hatchability, alongside alteration in energy metabolites, including decreased in lipid droplets, triglyceride and glycerol levels and increased diglyceride content. Additionally, sperm quantification revealed significant reductions in eupyrene sperm count following <em>ScLSD2</em> silencing, indicating impaired reproductive function. These results show the pivotal role of LSD2 in linking lipid metabolism and reproductive physiology in <em>S. cerealella</em>, positioning it as a promising target for pest management strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106417"},"PeriodicalIF":4.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silencing the β-glucan recognition protein enhanced the pathogenicity of Cordyceps fumosorose against Hyphantria cunea Drury larvae","authors":"Wenxuan Li ,&nbsp;Jianyang Bai ,&nbsp;Jundan Deng ,&nbsp;Weikang Xu ,&nbsp;Qing-He Zhang ,&nbsp;Jacob D. Wickham ,&nbsp;Mengting Wu ,&nbsp;Longwa Zhang","doi":"10.1016/j.pestbp.2025.106415","DOIUrl":"10.1016/j.pestbp.2025.106415","url":null,"abstract":"<div><div>The β-glucan recognition protein (<em>βGRP</em>) plays a crucial role in pathogen recognition by insects, thereby activating their innate immunity. However, the immune response of <em>βGRP</em> in <em>Hyphantria cunea</em> Drury (Lepidoptera: Noctuidae), an invasive pest of forests and agriculture, to pathogens remains unclear. In this study, we identified a new isolate of the entomopathogenic fungus <em>Cordyceps fumosorosea</em>. We found <em>C. fumosorosea</em> exhibits significant pathogenicity against <em>H. cunea</em> larvae. Based on the transcriptome, we found that the <em>βGRP</em> genes of <em>H. cunea</em> can be induced to express after infection by <em>C. fumosorosea</em>. <em>βGRP1</em> is primarily expressed in the fat body and significantly upregulated by 11.23-fold at 12 h post-infection with <em>C. fumosorosea</em>. Besides, molecular docking showed a potential binding interaction between <em>β</em>GRP1 protein and β-1,3-glucans, which is further confirmed by protein-carbohydrate binding assays. Additionally, the knockdown of <em>βGRP1</em> through RNA interference increases the mortality of <em>H. cunea</em> larvae following <em>C. fumosorosea</em> infection. Taken together, our study underscores the critical role of <em>βGRP1</em> in the immune response to <em>C. fumosorosea</em> infection and suggests an integrated pest management strategy that combines entomopathogenic fungi with RNA interference technology as an effective approach for controlling <em>H. cunea</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106415"},"PeriodicalIF":4.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NADPH-cytochrome P450 reductase mediates resistance to neonicotinoid insecticides in Bradysia odoriphaga","authors":"Xinxiang Wang,&nbsp;Xianglong Chen,&nbsp;Taoling Zhou,&nbsp;Wu Dai,&nbsp;Chunni Zhang","doi":"10.1016/j.pestbp.2025.106406","DOIUrl":"10.1016/j.pestbp.2025.106406","url":null,"abstract":"<div><div>As a crucial electron transfer partner of the P450 system, NADPH-cytochrome P450 reductase (CPR) plays an influential role in P450-mediated detoxification metabolism of xenobiotics. CPR has been found to be associated with insecticide resistance in several insects. However, the role of CPR in the cross-resistance of <em>Bradysia odoriphaga</em> to clothianidin and neonicotinoid insecticides remains to be elucidated. In this study, the <em>CPR</em> gene (<em>BoCPR</em>) of <em>B. odoriphaga</em> was cloned and characterized. The expression of <em>BoCPR</em> was more abundant in the adult stage and in the midgut and Malpighian tubules of larvae, and <em>BoCPR</em> was significantly overexpressed in the clothianidin-resistant (CL-R) strain compared to the susceptible (SS) strain. Exposure to clothianidin significantly increased <em>BoCPR</em> expression in both the SS and CL-R strains. In addition, knockdown of <em>BoCPR</em> in SS and CL-R strains significantly reduced CPR and P450 enzyme activities, and resulted in a significant increase in larval susceptibility to clothianidin, imidacloprid, and thiamethoxam. These results suggest that <em>BoCPR</em> plays an important role in <em>B. odoriphaga</em> resistance to clothianidin and cross-resistance to neonicotinoid insecticides.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106406"},"PeriodicalIF":4.2,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicological effects of chemical pesticides in fish: Focusing on intestinal injury and gut microbial dysbiosis","authors":"Bizhang Dong ,&nbsp;Hyo-Bang Moon","doi":"10.1016/j.pestbp.2025.106405","DOIUrl":"10.1016/j.pestbp.2025.106405","url":null,"abstract":"<div><div>The gut is susceptible to environmental pollutants and is a crucial barrier to exchanging internal and exterior substances in animals and humans. Intestinal microbiota plays vital roles in nutrition metabolism, synthesis of functional compounds, immune regulation, inflammation, and infection. Gut microbiota dysbiosis can induce intestinal physical barrier damage, trigger inflammation, and increase gut permeability. Intestinal barrier dysfunction facilitates the entry of pathogenic bacteria and harmful chemicals into the body through the blood circulation system, potentially causing neurotoxicity, hepatotoxicity, respiratory toxicity, growth inhibition, and even death. Herein, we overviewed the knowledge on the toxic effects of chemical pesticides on fish intestines and gut microbiota in the latest decade (2015–2025) and attempted to summarize the potential toxicological mechanisms. Chemical pesticide exposure can cause intestinal damage, impair immune function, and disrupt gut microbiota in fish. Gut microbial dysbiosis was strongly associated with intestinal injury. Alterations in gut microbiome metabolites, such as lipopolysaccharide, peptidoglycan, and short-chain fatty acids, have been linked to intestinal damage, inflammation, and changes in permeability. The mechanisms underlying intestinal injury in fish exposed to chemical pesticides included apoptosis, oxidative stress, and inflammation, which are mediated by reactive oxygen species pathways as well as death receptor and mitochondrial signaling pathways. Furthermore, pesticide-induced intestinal dysbiosis can cause neurotoxicity and hepatotoxicity through the microbiome-gut-brain/liver axis.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106405"},"PeriodicalIF":4.2,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and sex pheromone-binding characteristics of pheromone-binding protein 3 in Tuta absoluta (Lepidoptera: Gelechiidae)","authors":"Xiaolan Ou ,&nbsp;Xianya Li ,&nbsp;Bo Xu ,&nbsp;Yusheng Wang ,&nbsp;Guifen Zhang ,&nbsp;Wanxue Liu ,&nbsp;Fanghao Wan ,&nbsp;Hongbo Jiang ,&nbsp;Khalid Haddi ,&nbsp;Cong Huang ,&nbsp;Yibo Zhang","doi":"10.1016/j.pestbp.2025.106404","DOIUrl":"10.1016/j.pestbp.2025.106404","url":null,"abstract":"<div><div>The olfactory system plays a crucial role in insect survival and reproduction. Odorant-binding proteins (OBPs) are essential for odor discrimination and hold the potential to be targets for pest management. <em>Tuta absoluta</em> (Lepidoptera: Gelechiidae), a devastating invasive pest of Solanaceae crops, has limited research on its OBPs. In this study, 34 OBP genes were identified in <em>T. absoluta</em>, including <em>TabsGOBP1</em>, <em>TabsGOBP2</em>, <em>TabsPBP1a</em>, <em>TabsPBP1b</em>, <em>TabsPBP1c</em>, and <em>TabsPBP3</em>, which belong to the Lepidoptera-specific GOBP/PBP subclass. Expression profiling revealed <em>TabsPBP3</em> to be predominantly expressed in male antennae and the female pheromone gland-ovipositor complex, with peak expression at 6:00 AM associated with courtship and mating behavior. Fluorescence competitive binding assays demonstrated that TabsPBP3 strongly binds to the main pheromone component (3<em>E</em>, 8<em>Z</em>, 11<em>Z</em>)-tetradecatrien-1-yl acetate (TDTA) but exhibits weak or no affinity for other components. Molecular docking identified key active sites in TabsPBP3, including Phe37, Tyr61, Ile77, Leu84, Ile86, Leu87, Phe101, Ala136, Ile139, and Ala140, which facilitate interaction with TDTA. These findings establish TabsPBP3 as a key player in TDTA detection and provide foundational data for innovative pest control strategies targeting <em>T. absoluta</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106404"},"PeriodicalIF":4.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triflumezopyrim-induced changes in the flight ability and energy metabolism of the brown planthopper, Nilaparvata lugens","authors":"Jitong Li ,&nbsp;Lei Zhu ,&nbsp;Xinyi Lv ,&nbsp;Xin Zhou ,&nbsp;Xinyan Liang ,&nbsp;Yiping Wang ,&nbsp;Lin Chen ,&nbsp;Jinglan Liu","doi":"10.1016/j.pestbp.2025.106402","DOIUrl":"10.1016/j.pestbp.2025.106402","url":null,"abstract":"<div><div>The brown planthopper (BPH), <em>Nilaparvata lugens</em> Stål, is a migratory pest of rice that exhibits short- and long-winged polymorphisms. The increased prevalence of long-winged individuals signals either immigration from external sources or imminent emigration from local populations. The migratory behavior of BPH is contingent upon its intrinsic flight capabilities, which have historically been managed through chemical pesticides. However, the challenge of mitigating pesticide resistance and rebound in BPH populations poses a significant concern for agricultural stakeholders. In this investigation, triflumezopyrim (TFM) was shown to be an effective agent in diminishing the flight capacity of BPH. Sublethal (0.249 mg/L) and median lethal concentration (0.571 mg/L) of TFM were used to construct a transcriptional profile of the long-winged form of BPH at 72 h post-emergence. Furthermore, following TFM exposure, a marked reduction in the flight performance of BPH was observed, and this was accompanied by elevated trehalose levels, attenuated trehalase activity, and a decrease in glucose, glycogen, and total sugar concentrations. In response to TFM, there was a substantial increase in reactive oxygen species within flight muscles, heightened H₂O₂ levels, and a reduced GSH/GSSG ratio. Histopathological examination of flight muscle sections showed that the flight muscles of TFM-treated BPH exhibited a higher proportion of apoptotic cells as compared to controls, suggesting that TFM impairs the flight capability of BPH. This study elucidates how TFM exposure impacts the flight ability of long-winged BPH and provides transcriptomic data that help clarify the role of TFM role in pest management.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106402"},"PeriodicalIF":4.2,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of resveratrol on honeybee health: Mitigating pesticide-induced oxidative stress and enhancing detoxification","authors":"Linlin Liu ,&nbsp;Min Shi ,&nbsp;Yanyan Wu ,&nbsp;Jiali Hao ,&nbsp;Junxiu Guo ,&nbsp;Shanshan Li ,&nbsp;Pingli Dai ,&nbsp;Jing Gao","doi":"10.1016/j.pestbp.2025.106403","DOIUrl":"10.1016/j.pestbp.2025.106403","url":null,"abstract":"<div><div>The widespread use of pesticides poses a significant threat to honeybee health by impacting their survival, behavior, immune function, and detoxification capacity. While phytochemicals such as resveratrol (RSV) have shown potential in mitigating oxidative stress and enhancing antioxidant defenses, their role in improving honeybee tolerance to pesticide exposure remains underexplored. In this study, we investigated the effects of RSV supplementation on honeybees exposed to three pesticides: dinotefuran (DIN), tebuconazole (TEB), and deltamethrin (DEL). The results showed that RSV supplementation significantly improved survival, feed intake, mobility, and gustatory sensitivity, indicating its protective effects against pesticide toxicity. Furthermore, RSV helped normalize impaired detoxification enzyme activities, including SOD, POD, catalase, and glutathione reductase, and reduced ROS levels and lipid peroxidation. Gene expression analysis revealed that RSV modulates Toll pathway-related genes like <em>defensin</em> and <em>apidaecin</em>, alleviating immune suppression caused by pesticides. Additionally, RSV influenced the insulin/insulin-like growth factor signaling (IIS) pathway by reducing <em>ilp1</em> and <em>inr1</em> expression, potentially mitigating metabolic stress. These findings demonstrate that protective effects of RSV may be linked to its ability to counter oxidative stress, restore mitochondrial function, and enhance energy metabolism. Furthermore, RSV is widely available, cost-effective, and easily incorporated into bee feed, making it feasible for large-scale application. This study highlights the protective role of RSV in pesticide detoxification in honeybees, offering new perspectives for honeybee health management and environmental toxicology research. By reducing the adverse effects of pesticides on honeybees, the application of RSV not only contributes to maintaining ecological balance but also supports sustainable agricultural practices. Future research should focus on optimizing its dosage, evaluating long-term effects, and investigating its impact on colony dynamics to facilitate its practical implementation in apiculture.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106403"},"PeriodicalIF":4.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sub-chronic exposure of hexaconazole may induce metabolic and neuropathic diseases: The evidence from gut microbiota","authors":"Dali Sun ,&nbsp;Tianming Yang ,&nbsp;Min Wang ,&nbsp;Junxiao Pang ,&nbsp;Fumin Li","doi":"10.1016/j.pestbp.2025.106398","DOIUrl":"10.1016/j.pestbp.2025.106398","url":null,"abstract":"<div><div>The high-frequency detection and long persistence of hexaconazole (Hex) in agricultural products and environment poses potential risk to non-targeted organisms which should pay special attention to. Intestinal flora plays an important role in host health by prevention the occurrence of various diseases. Therefore, in this study, the disturbance of Hex on intestinal function and flora in rats had been studied at environmental related concentrations to evaluate the potential risk of Hex. Our results showed that Hex exposure induced serious oxidative stress and inflammation in intestinal tract. Meanwhile, it notably decreased the tight connectivity in colonic cell leading to the dysfunction of intestinal barrier. Moreover, 16sRNA gene sequencing showed that Hex exposure significantly disturbed the composition and structures of gut microbiota by decrease beneficial bacteria and increase pathogenic bacteria. Further, the metabolites and SCFAs that related to neuropathic and metabolic diseases in colonic contents were also significantly affected by Hex exposure. The pathways of membrane transport, replication and repair, lipid metabolism, and neurodegenerative diseases had been seriously interfered. The obtained results referred that Hex exposure may pose potential risk to metabolic system causing obesity, metabolic syndrome, and cardiovascular as well as nervous system inducing Parkinson's diseases, Alzheimer's diseases, and depression. Our study provided a new sight to study the mechanisms of Hex induced toxicity effects from the aspect of gut microbiota which could help for prevention the risk induced by Hex.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106398"},"PeriodicalIF":4.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insecticide-induced sublethal effect in the fall armyworm is mediated by miR-9993/miR-2a-3p - FPPS/JHAMT - JH molecular module","authors":"Aiyu Wang ,&nbsp;Yuanxue Yang ,&nbsp;Yun Zhang ,&nbsp;Chao Xue ,&nbsp;Yinjie Cheng ,&nbsp;Yifei Zhang ,&nbsp;Wenjuan Zhang ,&nbsp;Ming Zhao ,&nbsp;Jianhua Zhang","doi":"10.1016/j.pestbp.2025.106400","DOIUrl":"10.1016/j.pestbp.2025.106400","url":null,"abstract":"<div><div>The sublethal effect of insecticides can affect the population dynamics of pests by changing the physiological or behavioral changes, which poses a serious threat to the sustainable control of crop pests in the field. However, the molecular regulation mechanism that mediates the sublethal effect of insecticides on crop pests remains unsolved. Here, we show that the sublethal effect of spinetoram and cyantraniliprole on <em>Spodoptera frugiperda</em> is mediated by the molecular module of microRNA-9993/microRNA-2a-3p - farnesyl diphosphate synthase gene (<em>FPPS</em>)/juvenile hormone (JH) acid methyltransferase gene (<em>JHAMT</em>) - JH. Spinetoram prolonged the duration of larvae and pupae, decreased the weight of pupae, while cyantraniliprole prolonged the duration of larvae and decreased the emergence rate. Similarly, injection of the juvenile hormone analogue (JHA) methoprene significantly prolonged the developmental duration of larvae and pupae, decreased the pupal weight and emergence rate. This sublethal phenotypic change was due to the upregulation of key JH synthesis genes, including <em>FPPS</em> and <em>JHAMT</em>, mediated by spinetoram and cyantraniliprole, resulting in an increase in JH titer. Furthermore, it was confirmed by small RNA sequencing, dual luciferase analysis and agomir-miRNA injection, miR-9993 and miR-2a-3p that it could bind <em>FPPS</em> and <em>JHAMT</em> respectively, and regulated the expression level of <em>FPPS</em> and <em>JHAMT</em> to affect the titer of JH, thus changing the phenotype of <em>S. frugiperda</em>. Collectively, these results provide insights into the mechanism of insecticide regulation of sublethal effects of pests, expand our understanding of development-related miRNAs, and reveal key factors involved in JH signaling pathways that support sublethal effects of insecticides.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106400"},"PeriodicalIF":4.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}