Pesticide Biochemistry and Physiology最新文献

{"title":"Scale-up synthesis and antiviral mechanisms of novel fluorine-containing cytidine peptide compound SN15: Insights from transcriptomic and proteomic analyses","authors":"Miao Yu ,&nbsp;Chuantao Xu ,&nbsp;Yan Wang ,&nbsp;Shidong Zhou ,&nbsp;Jingwen Li ,&nbsp;Zihao Xia ,&nbsp;Mengnan An ,&nbsp;He Liu ,&nbsp;Yuanhua Wu","doi":"10.1016/j.pestbp.2025.106714","DOIUrl":"10.1016/j.pestbp.2025.106714","url":null,"abstract":"<div><div>Cytidine peptide compounds have emerged as promising candidates for antiviral agents, but their large-scale production and mode of action remain underexplored. By optimizing temperature control and stirring efficiency, and replacing trifluoroacetic acid with an HCl/dioxane system for tert-butyloxycarbonyl (Boc) deprotection, efficient preparation of SN15 was achieved with a 78.47 % yield without the need for column chromatographic purification. Antiviral assays showed that SN15 inhibited TMV accumulation in single cells by 53.44 % at 250 μg/mL and significantly suppressed systemic infections by PVY, PMMoV, and TuMV, reducing viral RNA accumulation by 54.16 %, 41.14 %, and 61.85 %, respectively. Transcriptomic analysis identified 9676 differentially expressed genes (DEGs), with ribosomal protein-related pathways significantly upregulated and hormone signaling/ubiquitination pathways downregulated. Proteomic analysis revealed 216 differentially expressed proteins (DEPs), including ribosomal components and stress-response proteins, consistent with transcriptomic trends. Results of TRV-VIGS showed that silencing of <em>60S ribosomal protein L6</em> or <em>lipid transfer-like protein VAS</em> significantly increased TMV accumulation, confirming its critical role in antiviral defense, while silencing of <em>peroxidase N1 precursor</em> and <em>E3 ubiquitin-protein ligase CIP8-like isoform X1</em> inhibited viral replication. This study reveals the molecular mechanism by which SN15 exerts antiviral effects through multi-pathway synergistic regulation, and provides a theoretical basis for the industrial production and broad-spectrum antiviral application of the fluorinated cytidine peptide compound SN15.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"216 ","pages":"Article 106714"},"PeriodicalIF":4.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and toxicity analysis of Lepidopteran toxic novel Bacillus thuringiensis strain T407","authors":"Rajadurai Gothandaraman ,&nbsp;Raghu Rajasekaran ,&nbsp;Jayakanthan Mannu ,&nbsp;Balasubramani Venkatasamy ,&nbsp;Kokiladevi Eswaran ,&nbsp;Mohankumar Subbarayalu","doi":"10.1016/j.pestbp.2025.106718","DOIUrl":"10.1016/j.pestbp.2025.106718","url":null,"abstract":"<div><div><em>Bacillus thuringiensis</em> is a gram-positive and spore-forming bacterium recognized for its pathogenicity against a broad spectrum of insect and nematode pests, and it has been extensively employed as a biopesticide. In this study, we examined the morphological, molecular, and genetic features of a native <em>Bt</em> isolate T407, which demonstrated significant toxicity against lepidopteran pests. Scanning Electron Microscopy confirmed the presence of bipyramidal and cuboidal-shaped crystals in T407. SDS-PAGE examination of spore-crystal mixtures revealed two significant protein bands at ∼135 and ∼ 65 kDa, representing Cry1 and Cry2 proteins. The presence of <em>cry</em> and <em>vip</em> gene combinations in T407 was confirmed by PCR analysis. Probit analysis estimates the LC₅₀ values of 0.771, 1.379, 2.038, and 14.997 μg/mL against <em>Plutella xylostella, Helicoverpa armigera, Spodoptera litura,</em> and <em>Spodoptera frugiperda</em>, respectively. The draft genome assembly of the <em>Bt</em> isolate T407 consists of 228 scaffolds, encompassing a total of 6,148,454 bp, with an N50 value of 93,189 bp. A total of 13 plasmids were identified in this genome through BLAST search similarity analysis. Similarly, 6631 protein-coding genes were identified in this genome with 53 tRNA, 4 rRNA, and 5 ncRNA genes. Similarly, a total of 11 insecticidal toxin proteins such as Cry1Ac5, Cry1Aa18, Cry1Ac5, Cry1Aa6, Cry1Ia42, Cry2Aa9, Cry2Ab41, Vip3Aa86, Mpp46Ab1, Vpb4Ca1, and Spp1Aa1 were identified in this <em>Bt</em> genome. In addition to insecticidal toxin genes few other toxin proteins like Zwa5A, Zwa5B, Zwa6, ChitinaseC, InhA1, InhA2, Enhancin, and Bmp1 also identified in this <em>Bt</em> genome.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"216 ","pages":"Article 106718"},"PeriodicalIF":4.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking monocrotophos pollution: From environmental spread to biosensor-based detection and remediation","authors":"Narlawar Sagar Shrikrishna ,&nbsp;Drishya Prakashan ,&nbsp;Sonu Gandhi","doi":"10.1016/j.pestbp.2025.106712","DOIUrl":"10.1016/j.pestbp.2025.106712","url":null,"abstract":"<div><div>Monocrotophos, a widely used organophosphate insecticide, has long stood at the intersection of agricultural utility and environmental hazard. This review delves beyond its routine application to uncover the broader implications of <strong>monocrotophos pollution</strong> and its persistence in agroecosystems. From its physicochemical properties and usage patterns to its contamination of soil, water, and food, monocrotophos pollution continues to pose serious ecological and human health challenges. We explore classical and emerging detection methods, highlighting the promise of biosensors for rapid, on-site residue monitoring. Furthermore, the review discusses various remediation strategies, with a focus on bioremediation using microbial systems for sustainable degradation. This review underscores the urgent need for coordinated action in pollution monitoring, detection, and mitigation. Taken together, the story of monocrotophos is not just about a pesticide, but a reflection of the delicate balance between agricultural advancement and environmental responsibility.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106712"},"PeriodicalIF":4.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piperonyl butoxide induces a unique gene expression of both detoxification and lipid metabolism genes and is independent of nutrition, microbiota, and genetic background","authors":"Luke J. Pfannenstiel,&nbsp;Anastacia E. Dressel,&nbsp;Gabriel A.O. De Guzman,&nbsp;Jeffrey G. Scott ,&nbsp;Nicolas Buchon","doi":"10.1016/j.pestbp.2025.106708","DOIUrl":"10.1016/j.pestbp.2025.106708","url":null,"abstract":"<div><div>Piperonyl butoxide (PBO) is a commonly used insecticide synergist and cytochrome P450 (CYP) inhibitor. While its role in blocking xenobiotic metabolism is well established, we show here that PBO also triggers a unique transcriptional response in <em>Drosophila melanogaster</em>. This response includes genes classically associated with detoxification, such as <em>Cyps</em>, <em>Gsts</em>, and <em>Ugts</em>, as well as genes involved in lipid metabolism, revealing a novel physiological program that integrates detoxification with broader metabolic regulation. Unlike other xenobiotic exposures, the PBO-induced transcriptome does not resemble a classical stress response. This transcriptional induction is independent of genetic background, diet composition or microbiota, and is partially recapitulated by myristicin, another methylenedioxyphenyl compound. Fine-scale dissection combined with RT-qPCR reveals that PBO-inducible genes exhibit distinct spatial expression patterns across multiple tissues, including the gut, Malpighian tubules, and fat body. Although most PBO inducible genes show the highest basal expression in the digestive tract, approximately half are primarily induced in the digestive tract, whereas the others are predominantly induced in the fat body of the carcass. Using CRISPR-Mediated Integration Cassette <em>Gal4</em> reporter lines, we visualized the cell-specific expression of two PBO-induced <em>Cyps</em>, <em>Cyp4e2</em> and <em>Cyp28a5</em>, which display complementary and regionally restricted activation in the gut and carcass. We find that patterns of expression are maintained during induction by PBO. Our findings suggest that CYP inhibition by PBO triggers a novel, systemic transcriptional response that extends beyond xenobiotic detoxification and may reflect a broader disruption of metabolic homeostasis.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106708"},"PeriodicalIF":4.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled release system of pesticide nanoparticles based on intelligent response: current status and development trend","authors":"Hongling Shi ,&nbsp;Yifei Guo ,&nbsp;Zhengqi Dong","doi":"10.1016/j.pestbp.2025.106710","DOIUrl":"10.1016/j.pestbp.2025.106710","url":null,"abstract":"<div><div>Nanopesticides have emerged as a promising strategy to improve pest management in plant cultivation. Conventional pesticide overuse often causes environmental contamination, pest resistance, and harmful residues in edible plant tissues, thereby threatening food safety and crop yield. In contrast, smart and responsive nanopesticide systems enable targeted release in the rhizosphere or phyllosphere, enhance pesticide utilization efficiency, and markedly lower residue accumulation. Building on these advantages, nanopesticide delivery systems are designed to provide sustained pest control during cultivation while minimizing pesticide residues. This review summarizes the main types of nanopesticide delivery systems, highlighting carrier materials and preparation methods, and discusses recent advances in their development. Particular attention is given to emerging trends in system design and their application for pest and disease management in plant cultivation. Despite the considerable potential of these systems, challenges remain regarding stability, environmental compatibility, and cost-effectiveness. Future research should focus on improving the efficiency, controllability, and affordability of nanopesticide formulations. Addressing these issues will accelerate their transition from laboratory exploration to practical application. Ultimately, nanopesticides hold great promise as sustainable solutions to enhance the safety of plant cultivation and ensure high-quality crop production.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"216 ","pages":"Article 106710"},"PeriodicalIF":4.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of ZJS-178 resistant mutants of Fusarium pseudograminearum","authors":"Zhiheng Zhou ,&nbsp;Dongya Shi ,&nbsp;Qiaowan Chen ,&nbsp;Chang Liu ,&nbsp;Guiping Zhang ,&nbsp;Haijun Ma ,&nbsp;Zhonghua Ma","doi":"10.1016/j.pestbp.2025.106709","DOIUrl":"10.1016/j.pestbp.2025.106709","url":null,"abstract":"<div><div><em>Fusarium pseudograminearum</em> is a major causal agent of Fusarium crown rot (FCR) in wheat worldwide. ZJS-178, a cyanoacrylate fungicide and phenamacril derivative, has shown strong inhibitory activity against <em>Fusarium</em> species. However, its resistance risk and underlying resistance mechanisms in <em>F. pseudograminearum</em> remain unexplored. In this study, three sensitive <em>F. pseudograminearum</em> isolates were subjected to fungicide adaptation in the laboratory, resulting in 35 ZJS-178 resistant mutants, 22 of which exhibited high-level resistance. Compared to their parental strains, resistant mutants displayed reduced fitness, including impaired mycelial growth, conidiation, conidial germination, deoxynivalenol production, and virulence. Cross-resistance analysis revealed a positive correlation between ZJS-178 and phenamacril, but no cross-resistance with fludioxonil, tebuconazole, carbendazim, or prochloraz. Sequence alignment and gene replacement assays identified 10 point-mutations in the <em>FpMyoI</em> gene (encoding myosin I) as the genetic basis for resistance. Molecular docking analysis further confirmed that these mutations interfere with fungicide binding. This study provides critical insights into the molecular basis of ZJS-178 resistance and offers valuable guidance for resistance monitoring and management strategies in FCR control.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"216 ","pages":"Article 106709"},"PeriodicalIF":4.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungicide-enhanced differentiation and multidrug resistance of Botrytis cinerea on greenhouse tomato","authors":"Bowen Zhang,&nbsp;Zhenren Yang,&nbsp;Chenhui Wang,&nbsp;Yuewei Sun,&nbsp;Yanjun Zhao,&nbsp;Qingchun Huang","doi":"10.1016/j.pestbp.2025.106707","DOIUrl":"10.1016/j.pestbp.2025.106707","url":null,"abstract":"<div><div><em>Botrytis cinerea</em>, a necrotrophic fungal pathogen, causes rotting of tomato at both pre-harvest and postharvest stages. Fungicide practices often lead to differential resistance in isolates, necessitating detailed differentiation profiles for effective control. This study tested the resistance of <em>B. cinerea</em> isolates to eight fungicides (boscalid (Bos), pyrimethanil (Pyr), cyprodinil (Cyp), iprodione (Ipr), procymidone, tebuconazole, prochloraz, and pyrisoxazole), evaluated fitness penalties in radial growth, conidial production, and pathogenicity, and primarily explored the differentiation mechanism via infection cushion (IC) formation, autophagy, and mitochondrial metabolism. Results revealed that isolates QN01 and TL03 showed resistance to Bos, Pyr, and Cyp, with EC₅₀ values of 38.53, 50.04, 11.95 μg/mL (QN01) and 23.58, 26.27, 8.16 μg/mL (TL03), respectively, and remained sensitive to other fungicides, exhibiting a Pyr^HRBos^MRCyp^R resistance phenotype, while other isolates were sensitive to all tested fungicides. All isolates were capable of thriving growth, but significant differed in conidia production, pathogenicity, and sensitivity to fungicides in conidial germination and tube elongation. Moreover, QN01 was highly surface-sensitive for IC production, with accumulated autophagy, while TL03 showed marked conidiation differentiation and higher citrate, oxalate, and phosphate levels. Upon iprodione treatment, QN01's citrate and phosphate levels increased while TL03's decreased, accompanying with QN01 depleting oxalate faster than TL03. The results highlighted the fungicide-enhanced differentiation and the mycelial growth-linked resistance among isolates, emphasizing dicarboximides, triazoles, and pyrisoxazole as effective agents for grey mold management.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106707"},"PeriodicalIF":4.0,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABC transporter BmWh2 enhances antiviral defense against baculovirus in silkworm via ATG upregulation","authors":"Dandan Feng ,&nbsp;Huizhen Guo ,&nbsp;Jie Li ,&nbsp;Jiayi Liu ,&nbsp;Dan Zhao ,&nbsp;Hui Zhao ,&nbsp;Xinyi He ,&nbsp;Qingyou Xia ,&nbsp;Liang Jiang","doi":"10.1016/j.pestbp.2025.106704","DOIUrl":"10.1016/j.pestbp.2025.106704","url":null,"abstract":"<div><div>Baculoviruses are widely used as biological pesticides for pest control. <em>Bombyx mori</em> nucleopolyhedrovirus (BmNPV) is a typical baculovirus that causes serious economic losses in the sericulture. ATP-binding cassette (ABC) transporters are widely distributed in various organisms, from viruses to humans, and play important roles in multiple biological processes. However, their roles in the interactions between viruses and insect hosts remain largely unknown. In this study, we used molecular cloning, reverse transcription-PCR, and quantitative PCR (qPCR) to investigate the function of the silkworm ABC transporter <em>BmWh2</em> during BmNPV infection. The results showed that <em>BmWh2</em> was upregulated after BmNPV infection. In BmE cells, knockout of <em>BmWh2</em> significantly increased viral DNA content, while overexpression of <em>BmWh2</em> had the opposite effects, indicating that <em>BmWh2</em> could inhibit BmNPV proliferation. A transgenic vector overexpressing <em>BmWh2</em> was constructed under the control of the A4 promoter, and two transgenic silkworm lines A4Wh2–1/2 were generated <em>via</em> embryo microinjection. qPCR analysis confirmed that the expression level of <em>BmWh2</em> in A4Wh2 silkworms was significantly increased. After oral infection with BmNPV, the mortality of A4Wh2–2 was 24 % lower than that of non-transgenic WT, and the viral DNA content in A4Wh2–2 was 8 % of that in WT. The mRNA levels of <em>ATG6</em> and <em>ATG8</em> were increased in both BmE cells overexpressing <em>BmWh2</em> and A4Wh2 silkworms, implying that <em>BmWh2</em> inhibited viral proliferation by increasing <em>ATG</em> expression. This study is the first report of an ABC transporter involved in antiviral immunity in <em>B. mori</em>, providing a theoretical reference for pesticide resistance.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106704"},"PeriodicalIF":4.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145108800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enantioselective evaluation of the chiral pesticide cyclaniliprole: Bioactivity, toxicity, and effects on strawberries flavor quality","authors":"Yuping Liu ,&nbsp;Ning Zhang ,&nbsp;Dier Song ,&nbsp;Rui Liu ,&nbsp;Yufan Nie ,&nbsp;Yue Zhang ,&nbsp;Wenwen Guan ,&nbsp;Wentao Zhu ,&nbsp;Zhiqiang Zhou ,&nbsp;Jinling Diao","doi":"10.1016/j.pestbp.2025.106700","DOIUrl":"10.1016/j.pestbp.2025.106700","url":null,"abstract":"<div><div>Chiral pesticide enantiomers often exhibit different bioactivities, ecotoxicity and environmental fate. In this study, the separation conditions were systematically optimized based on HPLC combined with chiral stationary phases. The novel chiral insecticide cyclaniliprole enantiomers were well separated using a CHIRALPAK AD-H column with hexane/isopropanol (80/20, <em>v</em>/v) as mobile phase at 1 mL/min flow rate and 25 °C column temperature. The absolute configuration of cyclaniliprole enantiomers was determined using density functional theory. Bioactivity and toxicity results showed <em>S</em>-CYP exhibited higher bioactivity against <em>Myzus persica</em> and <em>Bemisia tabaci</em> than <em>R</em>-CYP and racemate, while acute toxicity to <em>Daphnia magna</em> and <em>Apis mellifera</em> showed minimal enantioselectivity. The degradation half-life of <em>R</em>-CYP on strawberries (33.08d) was significantly higher than <em>S</em>-CYP (24.90d) and racemate (24.40d). Additionally, racemate and two enantiomers all promoted fruit growth and ripening by increasing GA, IAA, and ABA levels, with <em>S</em>-CYP showing more effective. However, fruit growth may come at the cost of sacrificing flavor. All three pesticide treatments triggered oxidative stress in strawberries, suppressed sugar-metabolism related genes expression, lowered soluble sugar content, and disrupted volatile synthesis, ultimately reducing fruit flavor. Moreover, cyclaniliprole and its enantiomers increased anthocyanins and vitamin C contents, but decreased total phenolic content. At the enantiomeric level, <em>S</em>-CYP suppressed volatiles more strongly but had less impact on sugars than <em>R</em>-CYP, and promoted nutrients more strongly. Therefore, from the perspective of strawberry fruit development and flavor quality, application of <em>S</em>-CYP was more superior. However, commercialization of the pure isomer requires further stereospecific data on its bioactivity, toxicity, and environmental behavior.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106700"},"PeriodicalIF":4.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Omics analysis reveals key genes mediating herbicide resistance in Digitaria sanguinalis","authors":"Yidi Guan,&nbsp;Liru Liu,&nbsp;Zhi Xiong,&nbsp;Caiwen Li,&nbsp;Chunyuan Liu,&nbsp;Yuan Sun,&nbsp;Mingshan Ji","doi":"10.1016/j.pestbp.2025.106693","DOIUrl":"10.1016/j.pestbp.2025.106693","url":null,"abstract":"<div><div><em>Digitaria sanguinalis</em> (L.) Scop is recognized as one of the most problematic agricultural weeds, with chemical control remaining a crucial management strategy. However, increased selection pressures have led to the emergence of resistant populations that reproduce and establish themselves as dominant communities, thereby severely jeopardizing crop yields. It is especially crucial to reveal the resistance mechanism of <em>D.Sanguinalis</em>, and the lack of weed histology resources has always been an obstacle to the study of resistance mechanism, nowadays, with the development of histology technology, the combination of multi-omics applied to the identification of weed resistance genes is becoming more and more perfect. In our previous study, we have preliminarily demonstrated that the resistance of <em>D.Sanguinalis</em> to ALS inhibitors is related to the increase of P450 enzyme activity. Here, we employed single-molecule real-time (SMRT) sequencing technology to obtain full-length transcripts of <em>D. sanguinalis.</em> Using DIA proteomics, we identified upregulated herbicide-metabolizing proteins, which were validated via PRM analysis. By integrating the transcriptomic and proteomic results, we identified <em>CYP709B2</em> and <em>CYP74B2</em> as key effector genes that mediate resistance in <em>D. sanguinalis</em>. We elucidated the resistance patterns and specific genes associated with <em>D. sanguinalis</em>, thereby enriching the bioinformatics resources available for this species and providing a foundation for herbicide-resistant weed management.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"215 ","pages":"Article 106693"},"PeriodicalIF":4.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}