Pesticide Biochemistry and Physiology最新文献_第3页

Insecticide-induced sublethal effect in the fall armyworm is mediated by miR-9993/miR-2a-3p - FPPS/JHAMT - JH molecular module 杀虫剂诱导的秋粘虫亚致死效应是由miR-9993/miR-2a-3p - FPPS/JHAMT - JH分子模块介导的

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-04-01 DOI: 10.1016/j.pestbp.2025.106400

Aiyu Wang , Yuanxue Yang , Yun Zhang , Chao Xue , Yinjie Cheng , Yifei Zhang , Wenjuan Zhang , Ming Zhao , Jianhua Zhang

{"title":"Insecticide-induced sublethal effect in the fall armyworm is mediated by miR-9993/miR-2a-3p - FPPS/JHAMT - JH molecular module","authors":"Aiyu Wang , Yuanxue Yang , Yun Zhang , Chao Xue , Yinjie Cheng , Yifei Zhang , Wenjuan Zhang , Ming Zhao , Jianhua Zhang","doi":"10.1016/j.pestbp.2025.106400","DOIUrl":"10.1016/j.pestbp.2025.106400","url":null,"abstract":"<div><div>The sublethal effect of insecticides can affect the population dynamics of pests by changing the physiological or behavioral changes, which poses a serious threat to the sustainable control of crop pests in the field. However, the molecular regulation mechanism that mediates the sublethal effect of insecticides on crop pests remains unsolved. Here, we show that the sublethal effect of spinetoram and cyantraniliprole on <em>Spodoptera frugiperda</em> is mediated by the molecular module of microRNA-9993/microRNA-2a-3p - farnesyl diphosphate synthase gene (<em>FPPS</em>)/juvenile hormone (JH) acid methyltransferase gene (<em>JHAMT</em>) - JH. Spinetoram prolonged the duration of larvae and pupae, decreased the weight of pupae, while cyantraniliprole prolonged the duration of larvae and decreased the emergence rate. Similarly, injection of the juvenile hormone analogue (JHA) methoprene significantly prolonged the developmental duration of larvae and pupae, decreased the pupal weight and emergence rate. This sublethal phenotypic change was due to the upregulation of key JH synthesis genes, including <em>FPPS</em> and <em>JHAMT</em>, mediated by spinetoram and cyantraniliprole, resulting in an increase in JH titer. Furthermore, it was confirmed by small RNA sequencing, dual luciferase analysis and agomir-miRNA injection, miR-9993 and miR-2a-3p that it could bind <em>FPPS</em> and <em>JHAMT</em> respectively, and regulated the expression level of <em>FPPS</em> and <em>JHAMT</em> to affect the titer of JH, thus changing the phenotype of <em>S. frugiperda</em>. Collectively, these results provide insights into the mechanism of insecticide regulation of sublethal effects of pests, expand our understanding of development-related miRNAs, and reveal key factors involved in JH signaling pathways that support sublethal effects of insecticides.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106400"},"PeriodicalIF":4.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mode of toxicity of the β-triketone leptospermone to Aedes aegypti mosquitoes β-三酮细精子素对埃及伊蚊的毒性作用模式

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-04-01 DOI: 10.1016/j.pestbp.2025.106401

Sarah E. McComic , Rui Chen , Shova Mishra , Werner J. Geldenhuys , Charles L. Cantrell , Edwin R. Burgess IV , Troy D. Anderson , Daniel R. Swale

{"title":"Mode of toxicity of the β-triketone leptospermone to Aedes aegypti mosquitoes","authors":"Sarah E. McComic , Rui Chen , Shova Mishra , Werner J. Geldenhuys , Charles L. Cantrell , Edwin R. Burgess IV , Troy D. Anderson , Daniel R. Swale","doi":"10.1016/j.pestbp.2025.106401","DOIUrl":"10.1016/j.pestbp.2025.106401","url":null,"abstract":"<div><div>Leptospermone, a natural <em>β</em>-triketone and major constituent of manuka oil (<em>Leptospermum scoparium</em>), is an established inhibitor of plant HPPD and was identified to induce rapid knockdown and induce high toxicity to <em>Aedes aegypti</em> adults via topical and tarsal contact exposure with LD<sub>50</sub> values of 150 ng/mg of mosquito and 357 ng/cm<sup>2</sup>, respectively. Although toxic to mosquitoes, leptospermone was non-toxic to ticks, the honey bee, or the fruit fly indicating a high degree of insect specificity. Importantly, leptospermone was equally toxic to non-blood fed and blood-fed mosquitoes suggesting the mode of action is not via HPPD inhibition. Molecular modeling suggested high structural similarities between leptospermone and mammalian sulfonamide carbonic anhydrase (CA) inhibitors. In vitro potency assays with mosquito midgut homogenate or purified CA verify leptospermone inhibits <em>Ae. aegypti</em> CA, but not mammalian CAs. CAs are metalloenzymes that regulate the pH of tissues and ubiquitously expressed throughout insect tissues but are abundantly expressed in the mosquito midgut and, thus, we tested leptospermone to alter pH regulation in the mosquito midgut. Indeed, leptospermone significantly reduced the pH of <em>Ae. aegypti</em> midguts when compared to control mosquitoes which further supports the notion that leptospermone mode of action in insects is via inhibition of CA. These data verify leptospermone is an effective mosquitocide that induces rapid knockdown and toxicity to <em>Ae. aegypti</em> at doses that approach natural pyrethrins against pyrethroid-resistant mosquito strains. Further, the data indicate leptospermone mode of action is CA inhibition, which is a novel mosquitocide target and is different when compared to the mode of action in plants.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106401"},"PeriodicalIF":4.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison on the conformation folding and structure change of serum albumin induced by methyl parathion and its metabolite p-nitrophenol 甲基对硫磷及其代谢物对硝基酚诱导血清白蛋白构象折叠及结构变化的比较

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-30 DOI: 10.1016/j.pestbp.2025.106393

Lu Chen, Zhu Wang, Xianglong Wu, Qiulan Zhang, Yongnian Ni

{"title":"Comparison on the conformation folding and structure change of serum albumin induced by methyl parathion and its metabolite p-nitrophenol","authors":"Lu Chen, Zhu Wang, Xianglong Wu, Qiulan Zhang, Yongnian Ni","doi":"10.1016/j.pestbp.2025.106393","DOIUrl":"10.1016/j.pestbp.2025.106393","url":null,"abstract":"<div><div>Residues of organophosphorus pesticides (OPPs) and their metabolites pose potential risks to the environment and human health. In the work, multiple spectroscopy, atomic force microscope and computational simulations were utilized to compare the interaction between methyl parathion (MP) and its metabolite p-nitrophenol (PNP) with human serum albumin (HSA). The results showed that both MP and PNP spontaneously formed complexes with HSA predominantly facilitated by hydrogen bonds and van der Waals forces, following static quenching mechanisms. The binding constant of PNP (15.16 ± 0.10 × 10<sup>4</sup> L mol<sup>−1</sup>) with HSA was nearly 5 times larger than that of MP (3.58 ± 0.09 × 10<sup>4</sup> L mol<sup>−1</sup>), suggesting PNP had a stronger affinity with HSA, which was consistent with density functional theory (DFT) calculation. Molecular docking revealed that the binding energy of PNP (−4.54 kcal mol<sup>−1</sup>) was lower than that of MP (−4.07 kcal mol<sup>−1</sup>), which potentially contributed a longer in vivo half-life of PNP and greater potential harm. Moreover, synchronous, 3D, FTIR and CD spectroscopy analyses indicated that the binding of MP and PNP to HSA significantly altered the microenvironment of amino acid residues and the secondary structure of HSA. Molecular dynamics simulations further demonstrated these findings. The study provides insights on the interaction between the pesticide MP and its metabolite PNP with HSA, which help understand the impact of pesticide residues on the food safety and environmental protection at the molecular level.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106393"},"PeriodicalIF":4.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spirodiclofen inhibited melanin synthesis in zebrafish embryos 螺双氯芬抑制斑马鱼胚胎黑色素合成

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-29 DOI: 10.1016/j.pestbp.2025.106397

LiangHao Suo, FuYu Hou, ZiYu Wang, ChunHui Wu, Jia Xie, WeiGuo Miao, YongMei Fan, Jie Zhang

{"title":"Spirodiclofen inhibited melanin synthesis in zebrafish embryos","authors":"LiangHao Suo, FuYu Hou, ZiYu Wang, ChunHui Wu, Jia Xie, WeiGuo Miao, YongMei Fan, Jie Zhang","doi":"10.1016/j.pestbp.2025.106397","DOIUrl":"10.1016/j.pestbp.2025.106397","url":null,"abstract":"<div><div>Spirodiclofen has been registered and marketed in more than 50 countries worldwide and are widely used because of their broad-spectrum acaricidal activity and long-lasting efficacy. However, its environmental toxicological assessment to fish remains poorly understood. In the present study, zebrafish embryos were modelled and exposed to series concentration of spirodiclofen. It has been found that spirodiclofen exposure induced zebrafish embryos abnormal pigmentation, the quantitative analysis of melanin in images using Image J showed a significant decrease in the proportion of melanin area in zebrafish exposed to 0.146 mg/L treatment group at 48 and 96 h, respectively. ELISA analysis illustrated that zebrafish embryos exposed to 0.146 mg/L exhibited a significant decrease in the levels of melanin, tyrosinase and dopachrome tautomerase content, and in constant with these results, the genes involved in melanin synthesis (<em>Tyr</em>, <em>Dct</em> and <em>Pck-β</em>) were significantly downregulated, indicating that melanin synthesis was inhibited. The molecular docking showed that spirodiclofen had a lower binding energy with tyrosinase compared to other compounds. The results demonstrated that spirodiclofen interfered zebrafish embryos melanin synthesis. This provided new insights into the mechanism of spirodiclofen toxicity to zebrafish embryos.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106397"},"PeriodicalIF":4.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polystyrene-microplastics and Emamectin Benzoate co-exposure induced lipid remodeling by suppressing PPARα signals to drive ACSL4-dependent ferroptosis and carp splenic injury 聚苯乙烯-微塑料和苯甲酸埃维菌素共暴露通过抑制PPARα信号驱动acsl4依赖性铁下垂和鲤鱼脾损伤诱导脂质重塑

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-29 DOI: 10.1016/j.pestbp.2025.106396

Hao Wu , Tong Xu , Naixi Yang , Shiwen Xu

{"title":"Polystyrene-microplastics and Emamectin Benzoate co-exposure induced lipid remodeling by suppressing PPARα signals to drive ACSL4-dependent ferroptosis and carp splenic injury","authors":"Hao Wu , Tong Xu , Naixi Yang , Shiwen Xu","doi":"10.1016/j.pestbp.2025.106396","DOIUrl":"10.1016/j.pestbp.2025.106396","url":null,"abstract":"<div><div>Microplastics (MPs) and Emamectin Benzoate (EMB) were identified as hazardous environmental pollutants, frequently coexisting in aquatic ecosystems, posing potential risk in the immune system of human and animal. However, the hazards of concurrent exposed to MPs and EMB on the carp spleen, and the specific mechanisms remain unclear. Here, we employed MPs and EMB-exposed carp models, and cultured splenocytes in vitro, to demonstrate that PPARα signals suppression underlay MPs and EMB-induced carp spleen injury, based on transcriptomics and lipomics analysis. This suppression exacerbated the buildup of polyunsaturated fatty acid (PUFA), and promoted ACSL4 expression, resulting in increased lipid peroxidation. Further studies found that the accumulation of lipid peroxides predominantly occurred in the mitochondria, which evoked mitochondrial homeostasis imbalance and compromised mitochondrial function, thereby initiating ferroptosis. Additionally, enhancing PPARα signaling, inhibiting ACSL4, or scavenging mitochondrial ROS was favor of mitigating accumulation of lipid peroxides, reducing mitochondrial damage and inhibiting ferroptosis. Notably, MPs and EMB co-exposure caused more severe damage than single exposure. These findings uncovered a potential mechanism, involving PPARα signaling inhibition by MPs and EMB co-exposure, which evoked lipid remodeling and increased ACSL4, to drive ferroptosis and carp splenic injury. This study highlighted the potential hazards to the aquaculture environments where co-exposure of MPs and EMB and provided reference for environmental toxicology research and the sustainable development of the aquaculture industry.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106396"},"PeriodicalIF":4.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The insecticidal toxicity of thymol and carvacrol to brown planthopper and the novel control strategy based on their disruption of the cuticular hydrophobicity 百里香酚和香芹酚对褐飞虱的杀虫毒性及基于其破坏褐飞虱表皮疏水性的新防治策略

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-28 DOI: 10.1016/j.pestbp.2025.106395

Lei Peng , Die Lu , Jiale Chen , Xiaoyan Hu , Zheng Fang , Yan Zhao

{"title":"The insecticidal toxicity of thymol and carvacrol to brown planthopper and the novel control strategy based on their disruption of the cuticular hydrophobicity","authors":"Lei Peng , Die Lu , Jiale Chen , Xiaoyan Hu , Zheng Fang , Yan Zhao","doi":"10.1016/j.pestbp.2025.106395","DOIUrl":"10.1016/j.pestbp.2025.106395","url":null,"abstract":"<div><div>The brown planthopper (<em>Nilaparvata lugens</em> (Stål)) is a major pest in rice cultivation. Due to its rapid reproduction and quick resistance development, current control strategies rely heavily on chemical insecticides. This has led to resistance in brown planthoppers to various commonly used insecticides. The limitations of traditional chemical control methods highlight the urgent need for environmentally friendly and sustainable alternatives. This study evaluated the contact toxicity, fumigant toxicity, and spray treatment effects of thymol, carvacrol, and five other volatile compounds against <em>N. lugens</em> nymphs. Results showed that thymol and carvacrol exhibited the strongest insecticidal effects. Both the two compounds, derived from plant essential oils, are low in toxicity and significantly disrupted the hydrophobicity of the nymphs' cuticles, converting them from hydrophobic to hydrophilic. Spray experiments further confirmed their insecticidal efficacy, and rinsing <em>N. lugens</em> after spray treatment with both compounds significantly increased <em>N. lugens</em> mortality rate. GC–MS analysis revealed interactions between carvacrol and cuticular hydrophobic compounds, such as octyl palmitate. These findings suggest that thymol and carvacrol could serve as effective green bioinsecticides by targeting the cuticle and internal compounds of <em>N. lugens</em>. In this study, a novel pest control strategy for rice field pests such as <em>N. lugens</em> was proposed, aiming to disrupt their cuticular hydrophobicity, thereby preventing them from escaping when falling into water and leading to drown in the field water. This method holds promise for significantly reducing the dependence on toxic pesticides and lowering pesticide usage.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106395"},"PeriodicalIF":4.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cythochrome P450-mediated dinotefuran resistance in onion thrips, Thrips tabaci 细胞色素p450介导的洋葱蓟马、烟草蓟马对敌百虫的抗性

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-28 DOI: 10.1016/j.pestbp.2025.106399

Akiya Jouraku , Koichi Hirata , Seigo Kuwazaki , Fumiya Nishio , Hajime Shimomura , Tomoya Yokoyama , Hisao Kusano , Motonori Takagi , Kanako Shirotsuka , Manabu Shibao , Hiroyuki Iida

{"title":"Cythochrome P450-mediated dinotefuran resistance in onion thrips, Thrips tabaci","authors":"Akiya Jouraku , Koichi Hirata , Seigo Kuwazaki , Fumiya Nishio , Hajime Shimomura , Tomoya Yokoyama , Hisao Kusano , Motonori Takagi , Kanako Shirotsuka , Manabu Shibao , Hiroyuki Iida","doi":"10.1016/j.pestbp.2025.106399","DOIUrl":"10.1016/j.pestbp.2025.106399","url":null,"abstract":"<div><div>Onion thrips, <em>Thrips tabaci</em>, have developed resistance to many insecticides, and over the last decade, resistant populations have spread widely across Japan. The cytochrome P450 (CYP) family, a widely conserved detoxification enzyme that metabolizes xenobiotics such as insecticides and phytochemicals, is believed to play important roles in the development of resistance in <em>T. tabaci</em>. However, CYPs involved in insecticide resistance in <em>T. tabaci</em> remain unclear. To comprehensively identify CYPs in <em>T. tabaci</em>, the genome sequences of the thelytokous <em>T. tabaci</em> (ANO strain) were constructed, and 18,965 genes (protein coding) were predicted. We identified 127 CYP genes in the predicted gene set by manual curation, and 38 of these CYP genes belonged to the CYP3 clan, including genes from the CYP6 family, which is one of the most important CYP families involved in resistance to neonicotinoids in many insect pests. To identify the CYPs involved in resistance to dinotefuran, which is one of the neonicotinoids used to control <em>T. tabaci</em>, RNA sequencing of dinotefuran-resistant and dinotefuran-susceptible strains was performed. Results revealed that, <em>TtCYP3652A1</em>, which belongs to the thrips-specific CYP3652A subfamily in the CYP3 clan, was significantly upregulated in the resistant strain. In vitro CYP metabolism assays using insect cells were conducted for <em>TtCYP3652A1</em> and five highly expressed CYP6 genes. Only TtCYP3652A1 significantly metabolized dinotefuran, which is considered to contribute to detoxification of dinotefuran. As no amino acid mutations were identified in the known target-site genes of neonicotinoids, <em>TtCYP3652A1</em> was considered to be the main factor involved in the resistance to dinotefuran.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106399"},"PeriodicalIF":4.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GRAS salts in postharvest fruit preservation: Disease control, freshness retention, and application mode GRAS盐在水果采后保鲜中的应用：疾病控制、保鲜及应用方式

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-25 DOI: 10.1016/j.pestbp.2025.106386

Juan Zhao , Yumei Wang , Kangyuan Xu , Jing He , Junjie Yi

{"title":"GRAS salts in postharvest fruit preservation: Disease control, freshness retention, and application mode","authors":"Juan Zhao , Yumei Wang , Kangyuan Xu , Jing He , Junjie Yi","doi":"10.1016/j.pestbp.2025.106386","DOIUrl":"10.1016/j.pestbp.2025.106386","url":null,"abstract":"<div><div>As consumers become increasingly aware of the nutritional and market value of fruit, postharvest decay and nutrient loss significantly reduce the economic value of fresh agricultural products. Traditionally, physical, chemical, and biological treatments have been employed to mitigate these issues. However, concerns over cost, safety, and environmental pollution have prompted the search for safer postharvest solutions. Generally Recognized As Safe (GRAS) salts, known for their safety and environmental friendliness, offer multiple benefits, including antimicrobial properties, the induction of fruit resistance, extended shelf life, and exemption from residue tolerances on agricultural commodities. This study aims to analyze the current understanding of the impact of postharvest application of GRAS salts on fruit quality and their inhibitory effects on postharvest pathogens, while also elucidating their mechanisms of action. The review begins with an overview of the legislative framework surrounding the use of GRAS salts, detailing their specific applications across various fruits and describing the different compounds involved. The focus then shifts to the antimicrobial mechanisms of GRAS salts and their effectiveness in different fruits. Finally, the study discusses the practical applications of GRAS salts in the industry and explores their future prospects in postharvest preservation. This review highlights the potential of GRAS salts in reducing losses and maintaining fruit quality, encouraging further research on their practical applications.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"211 ","pages":"Article 106386"},"PeriodicalIF":4.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organic amendments restore soil biological properties under pesticides application 施用农药后，有机改进剂可恢复土壤的生物特性

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-25 DOI: 10.1016/j.pestbp.2025.106394

Ahmad Latif Virk , Awais Shakoor , Naeem Ahmad , Huaqiang Du , Scott X. Chang , Yanjiang Cai

{"title":"Organic amendments restore soil biological properties under pesticides application","authors":"Ahmad Latif Virk , Awais Shakoor , Naeem Ahmad , Huaqiang Du , Scott X. Chang , Yanjiang Cai","doi":"10.1016/j.pestbp.2025.106394","DOIUrl":"10.1016/j.pestbp.2025.106394","url":null,"abstract":"<div><div>Pesticides are indispensable in modern agriculture, but they also affect soil biological properties and soil sustainability. Although organic amendments (OAs) have the potential to alleviate the negative effects of pesticides and restore soil biological properties, but results have been inconsistent. Here, we conducted a meta-analysis to study the overall effects of OAs on soil biological properties under pesticide application. Our results showed that sole pesticide application significantly decreased soil enzymatic activities (i.e., by −2.5 % for urease, −22 % for β-glucosidase, and − 10 % for dehydrogenase) and reduced microbial population size (e.g., by −6 % for microbial biomass, −10 % for actinomycetes population, −7 % for bacteria and − 12 % for fungi population). Noticeably, sole fungicide application did not significantly affect soil bacteria, fungi and actinomycetes populations as compared to sole herbicide and insecticide. In contrast, pesticide with OAs application significantly increased soil respiration (+60 %), activities of dehydrogenase (+73 %), urease (+80 %) and β-glucosidase (+65 %), microbial biomass (+79 %), bacterial (+65 %) and fungal (+13 %) population sizes than sole pesticide. However, soil acid phosphatase activity significantly decreased (−17 %) by using pesticide with OAs. Our results suggest that OAs in combination with pesticides can modify soil physicochemical properties and subsequently restore soil biological properties, but the magnitude of improvement of specific OAs in combination with individual pesticide classes remains unknown and should be studied in future research.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106394"},"PeriodicalIF":4.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silica and mesoporous silica nanoparticles display effective insecticidal effect and augment plant defense responses 二氧化硅和介孔二氧化硅纳米颗粒显示出有效的杀虫效果，增强了植物的防御反应

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106389

Deepali Choudhary , Sneha Deshmukh , G. Maheswari , Archana Kumari , Vandana Ghormade

{"title":"Silica and mesoporous silica nanoparticles display effective insecticidal effect and augment plant defense responses","authors":"Deepali Choudhary , Sneha Deshmukh , G. Maheswari , Archana Kumari , Vandana Ghormade","doi":"10.1016/j.pestbp.2025.106389","DOIUrl":"10.1016/j.pestbp.2025.106389","url":null,"abstract":"<div><div>The polyphagous insect pest, <em>Spodoptera litura</em> displays insecticide resistance that requires new control tactics. In this context, inorganic silica nanoparticles (SiNPs) and mesoporous silica nanoparticles (MSNPs) were studied for their insecticidal activity and their effects on the plant defense responses. The synthesized silica (SiNPs,160 nm size) and mesoporous silica (MSNPs,100 nm size) nanoparticles showed high insecticidal effect of against <em>S. litura</em> larvae with 73.0 and 80.0 % mortalities, respectively at low nanoparticles concentration (1 μg). Administration of NPs by feeding enhanced the larval gut uptake and caused a significant ∼14.9- to 12.7-fold reduction in lactate dehydrogenase activity for SiNPs and MSNPs, respectively. Efficient uptake of fluorescent NPs was illustrated in columnar larval gut cells. Feeding of SiNPs and MSNPs led to a significant reduction in larval weight (2.9- and 3.4-fold, respectively) due to their antifeedant effect which was positively correlated to larval mortalities. Both NPs exhibited negligible cytotoxicity in vitro. Furthermore, application of rhodamine B fluorescence-tagged NPs on soyabean leaves showed NPs presence on the leaf surfaces and were not internalized by the leaf. Moreover, the electromechanical plant responses to NPs application displayed increased localized signal durations (>2-fold). Additionally, SiNPs and MSNPs treatments significantly upregulated the <em>12-oxophytodienoate reductase</em> plant jasmonic acid defense pathway gene expression (2.7- and 1.4-fold, respectively) that led to enhanced jasmonic acid contents. Application of SiNPs and MSNPs at low concentrations achieved insecticidal effect against <em>S. litura</em> and enhanced the plant defense responses against pest. Silica nanoparticles have potential in safe and effective management of <em>S. litura</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106389"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0