Pesticide Biochemistry and Physiology最新文献

{"title":"Molecular hybridization of indoles and vanillins via α-hydroxyketone linking for discovering new inhibitor of PVY intercellular traffic","authors":"Feifei Chen,&nbsp;Jiao Li,&nbsp;Fenyan Wang,&nbsp;Ningning Zan,&nbsp;Mingshu Lou,&nbsp;Chunni Zhao,&nbsp;Baoan Song,&nbsp;Runjiang Song","doi":"10.1016/j.pestbp.2025.106446","DOIUrl":"10.1016/j.pestbp.2025.106446","url":null,"abstract":"<div><div>Potato virus Y (PVY) significantly reduces yield in economically important <em>Solanaceae</em> crops, while the limited availability of effective antiviral agents in the market highlights the necessity for exploring innovative antiviral lead structures. Herein, we disclose a new analogue of virucidal compounds, which are designed by molecular hybridization of natural indole and vanillin moieties. Most hybrid derivatives under bio-assay exhibit anti-PVY properties. Compound <strong>C7</strong>, in particular, shows superior inactivating effects to PVY, with a median effect concentration (EC₅₀) of 121.3 ± 4.9 μg/mL, which is significantly lower than that of controls ribavirin (EC₅₀ = 245.6 ± 10.3 μg/mL) and vanisulfane (EC₅₀ = 351.4 ± 4.8 μg/mL). The molecular docking result indicates formation of stable hydrogen-bonding between Arg²¹⁴ of PVY capsid protein (CP) and <strong>C7</strong>. Combining <em>in vitro</em> and <em>in vivo</em> assays allows Arg²¹⁴ to be identified as a functional residue modulating PVY systemic invasion in hosts. Further evidence provided by confocal microscope technique supports that <strong>C7</strong> behaves as a potential inhibitor of virion intercellular movement. This study contributes an available lead structure for chemical blocking of viral cell-to-cell movement.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"212 ","pages":"Article 106446"},"PeriodicalIF":4.2,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk assessment and resistant mechanism of Fusarium graminearum to fluopyram","authors":"Zhang Jie ,&nbsp;Sun SiYu ,&nbsp;Guo Yu ,&nbsp;Ren Fuhao ,&nbsp;Sheng Guilin ,&nbsp;Wu Huan ,&nbsp;Zhao Baoquan ,&nbsp;Cai Yiqiang ,&nbsp;Gu Chunyan ,&nbsp;Duan Yabing","doi":"10.1016/j.pestbp.2025.106449","DOIUrl":"10.1016/j.pestbp.2025.106449","url":null,"abstract":"<div><div>Fusarium head blight (FHB), caused by the <em>Fusarium graminearum</em> species complex, poses a significant threat to global wheat production and human health due to its high virulence and the ability to produce harmful secondary metabolites. Although various fungicides have been extensively used to control FHB, there is a critical need for more comprehensive information on the resistance of <em>F.</em> g<em>raminearum</em> to fluopyram in China. This study evaluated the sensitivity of <em>F.</em> g<em>raminearum</em> strains collected from five distinct regions to fluopyram. The EC₅₀ values for fluopyram ranged from 1.20 to 6.42 μg/mL, with an average value of 3.30 μg/mL. Additionally, fluopyram-resistant mutants were generated in the laboratory by chemical taming, with a resistance frequency of 8.3 × 10-⁻³. Furthermore, no significant differences were observed in conidiation, growth rate, and temperature sensitivity between the resistant mutants and the parental strains. It was also determined that fluopyram exhibits positive cross-resistance with pydiflumetofen but no cross-resistance with carbendazim, tebuconazole, and pyraclostrobin. The mutations associated with fluopyram resistance in <em>F.</em> g<em>raminearum</em> were identified as SDHB-H248L, SDHC2-A83V, and SDHC2-R86K. Based on these findings, the risk of resistance development in <em>F.</em> g<em>raminearum</em> to fluopyram was assessed as moderate. Additionally, it was found that the combination of prothioconazole and fluopyram is more effective than their individual use in field trials. Considering the role of fluopyram in inhibiting DON produced by <em>F.</em> g<em>raminearum</em>, it is recommended that fluopyram be used in conjunction with other highly effective fungicides to control FHB, thereby achieving both disease control and mycotoxin reduction.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"212 ","pages":"Article 106449"},"PeriodicalIF":4.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A symbiotic bacterium regulates the detoxification metabolism of deltamethrin in Aedes albopictus","authors":"Si-jia Deng ,&nbsp;Lei Tu ,&nbsp;Lin Li ,&nbsp;Ju-ping Hu ,&nbsp;Ju-lin Li ,&nbsp;Jian-xia Tang ,&nbsp;Mei-chun Zhang ,&nbsp;Guo-ding Zhu ,&nbsp;Jun Cao","doi":"10.1016/j.pestbp.2025.106445","DOIUrl":"10.1016/j.pestbp.2025.106445","url":null,"abstract":"<div><div>The mosquito <em>Aedes albopictus</em> is an important vector of dengue, chikungunya, and Zika; and is a globally distributed invasive mosquito with increasing resistance to insecticides, thereby posing a serious risk to global public health. Symbiotic gut bacteria have been shown to be related to insecticide resistance, but knowledge is still limited for <em>A. albopictus</em>. Here, we explored the role of <em>Serratia marcescens,</em> a gut symbiotic bacterium, in the resistance of <em>A. albopictus</em> to the insecticide deltamethrin. Using 16S-rRNA sequencing we found that <em>S. marcescens</em> was significantly enriched in <em>A. albopictus</em> after deltamethrin exposure, and that resistance increased after <em>S. marcescens</em> enrichment. The enzymatic activities of mixed-function oxidase (MFO) and glutathione S-transferase (GST), two important detoxification enzymes, were higher in the bacteria-enriched mosquitoes. The expressions of <em>ABCG4</em> and <em>GSTD1</em>, two genes related to detoxification metabolism, were up-regulated following <em>S. marcescens</em> infection and after deltamethrin exposure, as assayed using RNA-seq. The up-regulation of these two genes was most significant in midgut and Malpighian tubules. Our results suggest that <em>S. marcescens</em> infection could enhance deltamethrin resistance in <em>A. albopictus</em> by increasing detoxification metabolism; of interest for designing more efficient mosquito control measures.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"212 ","pages":"Article 106445"},"PeriodicalIF":4.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polysaccharide from discarded tobacco leaves and its anti-TMV activities","authors":"Yan Wang ,&nbsp;Zhengxuan Wang ,&nbsp;Yaping Liu ,&nbsp;Charles Brennan ,&nbsp;Jueting Liu ,&nbsp;Jinhong Zhou ,&nbsp;Wenbing Zhou ,&nbsp;Guiguang Cheng","doi":"10.1016/j.pestbp.2025.106443","DOIUrl":"10.1016/j.pestbp.2025.106443","url":null,"abstract":"<div><div>Natural polysaccharides have been attracted increasing attention in biopesticides for the antiviral effects. Tobacco leaves enriched the polysaccharides and had the potential against tobacco mosaic virus (TMV), however, the chemical structure and anti-TMV effect of polysaccharides from discarded tobacco leaves (DTCP) are still unclear. In this study, an optimized condition for extraction time of 3.5 h, temperature of 90 °C, and solid-liquid ratio of 1:45 led to the maximum extraction value of 21.11 % for DTCP. Subsequently, a homogeneous polysaccharide (DTP) of 3061 Da was purified from DTCP by DEAE and Sephadex G-100 column. The major monosaccharide composition of DTP was glucose (Glc), glucuronic acid (GlcA), galactose (Gal), and galacturonic acid (GalA) in a molar ratio of 3.9:2.6:1.06:0.84. NMR, methylation and IR analysis showed that DTP was a polysaccharide with →4)-<em>β</em>-D-Galp-(1 → 4)-<em>β</em>-D-Galp-(1 → 4)-<em>β</em>-D-Glcp as the main chain, and its branching points were composed of <em>α</em>-D-Glcp-(1→, <em>α</em>-D-GlcpA-(1→ and <em>α</em>-D-GalpA-(1→. Furthermore, DTP exhibited significant inactive and protective effects against TMV with the inhibitory rates of 80.40 % and 76.18 % at 500 μg/mL, respectively. DTP also interfered with the polymerization of TMV-CP to limit its self-assembly. Additionally, DTP could increase the superoxide dismutase, catalase, peroxidase and phenylalanine ammonia lyase activities of tobacco plants by 1.83, 2.73, 3.69 and 4.84 times, respectively, and could alleviate the hypersensitive response and systemic acquired resistance. Hence, DTP could serve as a new plant-derived pesticide for controlling TMV in agriculture.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"212 ","pages":"Article 106443"},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal activity and potential inhibition mechanism of 4-(diethylamino)salicylaldehyde against Rhizoctonia solani","authors":"Sishi Huang,&nbsp;Xiaoyue Yang,&nbsp;Jing Zhao,&nbsp;Muhammad Zeeshan,&nbsp;Chunjuan Wang,&nbsp;Desong Yang,&nbsp;Xiaoqiang Han,&nbsp;Guoqiang Zhang","doi":"10.1016/j.pestbp.2025.106444","DOIUrl":"10.1016/j.pestbp.2025.106444","url":null,"abstract":"<div><div><em>Rhizoctonia solani</em> is a significant soil-borne pathogenic fungus that poses a significant threat to the economically important agricultural crops. 4-(Diethylamino)salicylaldehyde (DSA) is a secondary metabolite produced by <em>Streptomyces</em> sp. KN37, which has antifungal activity, meanwhile its inhibitory mechanism is still unclear. In this study, we explored the antifungal efficacy of DSA and its potential mechanism of inhibiting <em>R. solani</em>. It was found that DSA exhibited significant antifungal activity against six tested plant pathogenic fungi, with <em>R. solani</em> being the most sensitive (EC₅₀ = 26.904 μg/mL). Notably, DSA effectively reduced the mycelial mass and inhibited sclerotia germination, demonstrating a good control efficacy of cucumber damping-off disease. Morphological observation showed that DSA significantly disrupted the shape and ultrastructure of the mycelium. Transcriptomic and metabolomic analyses revealed that DSA impacted the integrity of the cell membrane, redox processes, and energy metabolism in <em>R. solani</em>. The results of fluorescence staining, relative conductivity, H₂O₂ content, and antioxidant enzyme activity showed that the accumulation of ROS in hypha cells after DSA treatment possibly resulted in damage to cell membrane integrity. Furthermore, the reduction in ATP content, along with decreased ATPase and citrate synthase activity, indicates that energy production may be inhibited. Molecular docking analysis further showed that DSA may competitively inhibit citrate synthase, thereby inhibiting cell energy production and ultimately inducing apoptosis. Our study provides new insights into the potential mechanism by which DSA inhibits the mycelial growth of <em>R. solani</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"212 ","pages":"Article 106444"},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal activity and underlying mechanism of action of paeonol against Sclerotium rolfsii","authors":"Chunlan Shi ,&nbsp;Hongmei Li ,&nbsp;Dewei Yang ,&nbsp;Lirong Chen ,&nbsp;Kaichen Guan ,&nbsp;Xiaoping Qin ,&nbsp;Deqiang Qin ,&nbsp;Xi Gao ,&nbsp;Xiao Ding ,&nbsp;Guoxing Wu","doi":"10.1016/j.pestbp.2025.106436","DOIUrl":"10.1016/j.pestbp.2025.106436","url":null,"abstract":"<div><div>Southern blight caused by <em>Sclerotium rolfsii</em> threatens <em>Aconitum carmichaelii</em> cultivation. This study evaluated paeonol's antifungal activity and mechanisms against <em>S. rolfsii</em>. Using carrier toxicity assays, paeonol showed potent inhibition with an EC₅₀ of 42.54 μg/mL and 86.90 % suppression at 100 μg/mL. Minimum inhibitory (MIC) and fungicidal (MFC) concentrations were 400 μg/mL and 500 μg/mL, respectively. Scanning and transmission electron microscopy revealed paeonol-induced mycelial deformation, cytoplasmic disintegration, and organelle disruption. Biochemical assays indicated suppressed sugar metabolism enzymes, impaired protective enzyme activity, and increased leakage of nucleic acids and proteins, confirming membrane damage. Transcriptome analysis with GO/KEGG enrichment revealed paeonol disrupted cell membrane biosynthesis, inhibited energy metabolism, and interfered with enzymatic functions and biomolecule synthesis. Multi-level effects demonstrated paeonol compromises fungal viability through membrane destabilization and metabolic interference. These findings highlight paeonol's potential as an eco-friendly antifungal agent for southern blight management, warranting further agricultural development.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"212 ","pages":"Article 106436"},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to trans-anethole impairs reproduction and inhibits glutathione-S-transferase (GST) in engorged Rhipicephalus microplus female ticks (Acari: Ixodidae)","authors":"Aline Chaves Reis ,&nbsp;Isaac Filipe Moreira Konig ,&nbsp;Stefania Priscilla de Souza ,&nbsp;Naiara Melo ,&nbsp;Pedro Henrique Inácio Gomes ,&nbsp;Henrique Fernandes Silva ,&nbsp;Teodorico de Castro Ramalho ,&nbsp;Rafael Neodini Remedio","doi":"10.1016/j.pestbp.2025.106435","DOIUrl":"10.1016/j.pestbp.2025.106435","url":null,"abstract":"<div><div><em>Rhipicephalus microplus</em>, the cattle tick, is a species of great importance to veterinary medicine and is responsible for massive economic losses in agriculture. In our study, engorged <em>R. microplus</em> females were exposed to the natural product <em>trans</em>-anethole in concentrations ranging from 1 to 50 μL/mL, a commercial pesticide (positive control), 5 % DMSO (solvent), and water control. We evaluated a series of endpoints related to survival, reproductive parameters, ovicidal activity, ovary histopathology, oxidative stress, and an <em>in silico</em> analysis (docking and molecular dynamics) of glutathione S-transferase (GST) inhibition. Exposure to 50 μL/mL <em>trans</em>-anethole caused mortality above 90 %, whereas in the positive control, this value was around 45 %. The mean lethal concentration (LC₅₀) estimated for <em>trans</em>-anethole in engorged <em>R. microplus</em> seven days after the exposure was LC₅₀ = 27.14 μL/mL. A concentration-dependent response was observed in the percentage of control (%C) in ticks treated with <em>trans</em>-anethole, reaching %C = 82.87 at 25 μL/mL. However, only the positive control presented an ovicidal effect against <em>R. microplus</em>. The most prevalent morphological alterations in the ovaries were basophilic granules in the cytoplasm, irregular oocyte shape, and fusion of yolk granules. The score of these alterations at 10 μL/mL <em>trans</em>-anethole was statistically equivalent to the positive control. Exposure to 10 μL/mL <em>trans</em>-anethole led to reduced GST activity. <em>In silico</em> analysis revealed inhibition of GST by <em>trans</em>-anethole with a MolDock score of −80.66. Collectively, our data showed that <em>trans</em>-anethole impairs the reproduction of <em>R. microplus</em> and is a promising bioactive compound for tick control.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"212 ","pages":"Article 106435"},"PeriodicalIF":4.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular analysis of insecticide resistance mutation frequencies in five cockroach species from Korea","authors":"Hyun Kyu Shin ,&nbsp;Susie Cho ,&nbsp;Gang Chan Lee ,&nbsp;Do Eun Lee ,&nbsp;Heung Chul Kim ,&nbsp;In Yong Lee ,&nbsp;Chung Hyun Ko ,&nbsp;Soon-il Kim ,&nbsp;Seong Soo Kim ,&nbsp;Ju Hyeon Kim ,&nbsp;Si Hyeock Lee","doi":"10.1016/j.pestbp.2025.106434","DOIUrl":"10.1016/j.pestbp.2025.106434","url":null,"abstract":"<div><div>Cockroaches are major urban pests that pose public health risks due to their ability to transmit pathogens and develop insecticide resistance. In the Republic of Korea (ROK), resistance monitoring has traditionally relied on bioassays, with limited studies on resistance-associated mutations. This study examined the prevalence of <em>kdr</em> and <em>rdl</em> mutations, conferring resistance to pyrethroids and fipronil (a phenylpyrazole insecticide), respectively, in five cockroach species (<em>Blattella germanica</em>, <em>Blattella nipponica</em>, <em>Periplaneta americana</em>, <em>Periplaneta japonica</em>, and <em>Periplaneta fuliginosa</em>). A total of 1278 cockroaches from 243 regional samples were analyzed using quantitative sequencing. Results showed widespread resistance in <em>B. germanica</em>, with an overall frequency of 57.1 % for the <em>kdr</em> mutation (L993F) and 94.2 % for the <em>rdl</em> mutation (A302S) across 169 regional samples. No evidence of other <em>kdr</em> mutations, E434K or C764R, was found. Notably, <em>B. nipponica,</em> despite its recent emergence in indoor environments, exhibited no detectable resistance mutations, likely due to lower insecticide exposure in rural areas. Similarly, no <em>kdr</em> mutations were found in <em>Periplaneta</em> species, and the <em>rdl</em> mutation was detected in only a single <em>P. americana</em> regional sample, marking the first documented case. Given the increasing resistance to fipronil, alternative insecticides are needed for effective cockroach management. Dinotefuran, a neonicotinoid with limited prior use in cockroach control, is a promising candidate, as its toxic bait products are already available in the ROK. Broflanilide and isocycloseram also show potential but require regulatory approval. These findings highlight the need for ongoing resistance monitoring and proactive pest management strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"212 ","pages":"Article 106434"},"PeriodicalIF":4.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination of L-methionine and chlorantraniliprole enhances the abundance of opportunistic pathogenic bacteria in the intestine of greater wax moth leading to increased mortality risk","authors":"Zhaoyong Liu ,&nbsp;Dan Zhao ,&nbsp;Yue Wei","doi":"10.1016/j.pestbp.2025.106429","DOIUrl":"10.1016/j.pestbp.2025.106429","url":null,"abstract":"<div><div>Honey bees play an essential role in global crop production and agro-economic development due to their pollination properties. However, empirical evidence indicates a worldwide decline in bee colonies. The greater wax moth (GWM), a lepidopteran insect and natural enemy of honey bees, significantly contributes to this decline. Chlorantraniliprole (CH) is commonly used to control GWM in apiaries due to its efficacy and low toxicity to bees. However, long-term use of CH may lead to environmental pollution and GWM resistance. To enhance beekeeping safety and mitigate the risk of GWM resistance from prolonged CH use, we investigated the toxic effects of combining methionine (MET), which has demonstrated insecticidal activity against some lepidopteran pests, with CH on GWM. We conducted both individual and mixed exposure tests of MET and CH on GWM to finally evaluate the toxic effects of the combined treatment (MIX). The results indicated that the combination of MET and CH produced a synergistic lethal effect on GWM. Subsequent microbiome and immune-related gene expression assays, along with correlation analysis, revealed that the MIX treatment significantly reducing the abundance of <em>Enterococcus</em> spp., the primary genus in GWM, and induced immune stress in GWM. This phenomenon led to the proliferation of opportunistic pathogens such as <em>Pseudomonas</em> spp., ultimately leading to synergistic lethal effects on GWM mortality. This study provides new insights and data supporting the development of MET as a potential insecticide.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"212 ","pages":"Article 106429"},"PeriodicalIF":4.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription and functional analysis of phosphine fumigation regulating heat responses in Bactrocera dorsalis","authors":"Yisha Ma ,&nbsp;Li Li ,&nbsp;Hang Zou ,&nbsp;YongLin Ren ,&nbsp;Penghao Wang ,&nbsp;Tao Liu","doi":"10.1016/j.pestbp.2025.106433","DOIUrl":"10.1016/j.pestbp.2025.106433","url":null,"abstract":"<div><div>The oriental fruit fly, <em>Bactrocera dorsalis</em>, is a highly destructive pest in fruits, vegetables and flowers with heat treatment being common for its control in internationally traded produce. However, heat treatment can adversely affect quality, which limits its application. This study aimed to develop a novel control strategy combining phosphine fumigation with heat treatment (P + H), demonstrating a significant synergistic effect in controlling <em>B. dorsalis</em>. We conducted transcriptomic analysis and qRT-PCR validation to explore this synergistic effect. P + H significantly increased the expression of heat shock proteins (HSP), but their levels were lower than those induced by heat treatment alone. RNAi-based experiments confirmed the association between HSP gene expression and insect mortality, further supporting the important role of HSP genes in response to heat treatment. Functional enrichment analysis indicated that the MAPK/ERK signaling pathway and HSF-1 dephosphorylation dynamically regulate HSP genes expression under P + H treatment. Additionally, energy allocation toward cellular repair may further limit HSP gene synthesis, affecting insect tolerance to heat response. These findings provide insights into the molecular bases for the synergism of P + H treatment, highlighting that HSP gene regulation plays as critical role in insect stress resistance and offering a novel approach to pest control.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"212 ","pages":"Article 106433"},"PeriodicalIF":4.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143894508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}