Pesticide Biochemistry and Physiology最新文献

{"title":"Corrigendum to “Environmental RNAi-based reverse genetics in the predatory mite Neoseiulus californicus: Towards improved methods of biological control” [Pesticide Biochemistry and Physiology, Volume 180 (2022) 104993]","authors":"Noureldin Abuelfadl Ghazy , Takeshi Suzuki","doi":"10.1016/j.pestbp.2025.106348","DOIUrl":"10.1016/j.pestbp.2025.106348","url":null,"abstract":"","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"209 ","pages":"Article 106348"},"PeriodicalIF":4.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PEI-SWNT improves RNAi efficiency in Locusta migratoria via dsRNA injection delivery system","authors":"Qiurong Ren ,&nbsp;Qian Zhang ,&nbsp;Yangyang Liu ,&nbsp;Shuai Li ,&nbsp;Jianqin Zhang ,&nbsp;Yanli Wang ,&nbsp;Abeer El Wakil ,&nbsp;Bernard Moussian ,&nbsp;Jianzhen Zhang","doi":"10.1016/j.pestbp.2025.106361","DOIUrl":"10.1016/j.pestbp.2025.106361","url":null,"abstract":"<div><div>The instability of double-stranded RNA (dsRNA) restricts the application of RNA interference (RNAi) technology in agricultural pest management. Various types of nanocarriers have been developed and employed for the stable delivery of dsRNA. Nonetheless, it remains unclear which type of nanomaterial could deliver dsRNA stably and efficiently for gene knockdown in <em>Locusta migratoria</em>. In this study, we evaluated the ability of three biocompatible and low-toxicity inorganic nanomaterials—polyethylenimine (PEI)-functionalized single-walled carbon nanotube (PEI-SWNT), polyethylenimine-functionalized carbon quantum dots (PEI-CQDs), and layered double hydroxide (LDH)—to bind and stabilize dsRNA. The results revealed that, compared to PEI-CQDs and LDH, PEI-SWNT more effectively protected dsRNA from degradation in locust gut fluids, across various temperatures, and under different pH conditions. Furthermore, we investigated the efficacy of PEI-SWNT/dsRNA complexes in suppressing endogenous genes in locusts through both injection and oral administration methods. Compared to bare dsRNA, PEI-SWNT/dsRNA complexes enhanced RNAi efficiency by up to 46.0 % and increased mortality by up to 39.0 %. Moderate levels of PEI-SWNT could improve the germination rate of wheat, while not affecting leaf growth in the short term. To our knowledge, this study is the first to apply PEI-SWNT inorganic nanomaterials in insects, which provides a foundational basis and compelling evidence for the development of nanomaterial-based nucleic acid pesticides.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"209 ","pages":"Article 106361"},"PeriodicalIF":4.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143535056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution of UDP-glycosyltransferases to clothianidin resistance in Bradysia odoriphaga","authors":"Chunni Zhang,&nbsp;Ruifang Liu,&nbsp;Xianglong Chen,&nbsp;Xianling Du,&nbsp;Shiying Tang,&nbsp;Wu Dai","doi":"10.1016/j.pestbp.2025.106349","DOIUrl":"10.1016/j.pestbp.2025.106349","url":null,"abstract":"<div><div>Uridine diphosphate glycosyltransferases (UGTs) play critical roles in xenobiotic detoxification and are involved in insecticide resistance. In this study, UGT inhibitors, sulfinpyrazone and 5-nitrouracil, exhibited significant synergistic effects on clothianidin in the clothianidin-resistant strain (CL-R) of <em>Bradysia odoriphaga</em>. UGT enzyme content was significantly higher in the CL-R strain than in the susceptible strain (SS), and both the SS and CL-R strains showed a significant upregulation of UGT content after exposure to clothianidin. Two UGT genes, <em>UGT36M1</em> and <em>UGT306K1,</em> were significantly overexpressed in the CL-R strain. <em>UGT36M1</em> was predominantly expressed in the fat body, and <em>UGT306K1</em> exhibited high levels of expression in the Malpighian tubules and midgut. <em>UGT36M1</em> and <em>UGT306K1</em> in the SS strain were significantly upregulated in response to clothianidin exposure. The silencing of <em>UGT36M1</em> and <em>UGT306K1</em> significantly enhanced the susceptibility of <em>B. odoriphaga</em> larvae to clothianidin. Furthermore, transgenic overexpression of <em>UGT36M1</em> and <em>UGT306K1</em> in <em>Drosophila melanogaster</em> significantly increased the tolerance of fruit flies to clothianidin. These findings provide evidence of the crucial role of <em>UGT36M1</em> and <em>UGT306K1</em> in conferring resistance to clothianidin in <em>B. odoriphaga</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"209 ","pages":"Article 106349"},"PeriodicalIF":4.2,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transport mechanisms of pesticide mixtures impairing intestinal barrier function in mice","authors":"Ziqi Liu ,&nbsp;Kaijie Dai ,&nbsp;Zhiyong Cao ,&nbsp;Shujie Wang ,&nbsp;Shuyan Gui ,&nbsp;Wenyuan Hu ,&nbsp;Tingting Meng ,&nbsp;Yuying Liu ,&nbsp;Jinjing Xiao ,&nbsp;Haiqun Cao","doi":"10.1016/j.pestbp.2025.106356","DOIUrl":"10.1016/j.pestbp.2025.106356","url":null,"abstract":"<div><div>Multiple pesticide residues in the diet can be ingested through the intestine; however, the interactions affecting intestinal health remain unclear. Histopathological analysis of mouse intestines revealed synergistic damage in those exposed to a binary mixture of abamectin, fluazinam, and spirodiclofen. The combined exposure to fluazinam and spirodiclofen resulted in a 73.35 % reduction in the expression level of the tight junction protein claudin-1 in Caco-2 cells. The studies on the transport in Caco-2 cells revealed that the combined exposure to abamectin and spirodiclofen resulted in transport amounts that were 5.37 and 19.98 times greater than those observed with individual exposures, respectively. The transporter inhibitors and molecular docking analysis indicated that competitive inhibition of the breast cancer resistance protein (BCRP) led to decreased pesticide efflux. Therefore, the disruption of the intestinal barrier caused by the interaction of pesticide mixtures warrants attention when evaluating the safety of various pesticide residues.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"209 ","pages":"Article 106356"},"PeriodicalIF":4.2,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced immune response and antimicrobial activity in honey bees (Apis mellifera) following application of oxalic acid-glycerine strips","authors":"Eliška Pinďáková ,&nbsp;Silvie Dostálková ,&nbsp;Jana Jemelková ,&nbsp;Jana Fürstová ,&nbsp;Jana Hurychová ,&nbsp;Pavel Hyršl ,&nbsp;Dalibor Titěra ,&nbsp;Marek Petřivalský ,&nbsp;Pavel Dobeš ,&nbsp;Jiří Danihlík","doi":"10.1016/j.pestbp.2025.106353","DOIUrl":"10.1016/j.pestbp.2025.106353","url":null,"abstract":"<div><div>Bee health is influenced by multiple factors, including nutrition, immunity, and parasitic pressures. Since the spread of <em>Varroa destructor</em>, overwintering survival has significantly declined, making it one of the most serious threats to honey bee (<em>Apis mellifera</em> L.) populations worldwide. Natural acaricides, such as oxalic acid (OA), are widely employed for managing <em>Varroa</em> mites; however, their pharmacodynamics, particularly their impacts on honey bee physiology and immunity, remain insufficiently understood. We studied effects of oxalic acid on honey bee workers. The study compared three treatments: flumethrin, OA-glycerine strips (OA-G), and OA trickling (OA-T). Twelve colonies were divided into four groups, with samples collected at five time points (0, 24, 48, 72, and 192 h). Physiological changes were assessed through markers of oxidative stress, longevity, and immune parameters. Exposure to oxalic acid via glycerine strips induced a humoral immune response in adult bees. The antimicrobial activity of hemolymph and levels of antimicrobial peptides (abaecin, apidaecin, defensin, and hymenoptaecin) were elevated between 48 and 192 h after OA-G treatment compared to the control group. In contrast, these parameters were not influenced by OA-T or flumethrin treatment. These findings suggest that OA-G strips activate the honey bee's immune system, providing insights into broader implications of OA use in beekeeping. It is crucial to determine whether the activation of humoral immune systems has positive or negative effects, as well as to develop standardized and reliable treatment protocols that ensure both - health of colonies and their effectiveness in controlling <em>Varroa</em> mite infestations.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"209 ","pages":"Article 106353"},"PeriodicalIF":4.2,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143535055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of cytochrome P450 CYP71AF43 contributing resistance to fenoxaprop-P-ethyl in Alopecurus myosuroides from China","authors":"Junhui Tian ,&nbsp;Dingyi Bai ,&nbsp;Sifen He,&nbsp;Zongfang Li,&nbsp;Lianyang Bai,&nbsp;Lang Pan","doi":"10.1016/j.pestbp.2025.106355","DOIUrl":"10.1016/j.pestbp.2025.106355","url":null,"abstract":"<div><div>Black-grass (<em>Alopecurus myosuroides</em>), one of the most economically destructive herbicide-resistant weeds in Europe, is rapidly expanding in winter wheat regions of China. In recent years, the recommended application rate of fenoxaprop-P-ethyl in the field has failed to effectively control <em>Alopecurus myosuroides</em> populations, thereby threatening wheat yields at risk. In this study, we collected a suspected herbicide-resistant population (R-HB) of <em>Alopecurus myosuroides</em> from a wheat field in Hebei Province and confirmed its resistance to fenoxaprop-P-ethyl, with a resistance index of 26.73-fold. Sensitivity analyses of other ACCase-inhibiting herbicides revealed cross-resistance in the R-HB population to clethodim and pinoxaden. Molecular analysis indicated that the resistance phenotype in this population was not due to alterations in the target site. Pretreatment with the cytochrome P450 (P450) inhibitor malathion partially reversed fenoxaprop-P-ethyl resistance in the R-HB population. RNA-seq and RT-qPCR validation revealed the constitutive overexpression of the P450 gene <em>CYP71AF43</em> in the R-HB population. Molecular docking predictions suggest that the CYP71AF43 protein may have metabolic activity toward fenoxaprop-P-ethyl. In genetically modified yeast, overexpression of <em>AmCYP71AF43</em> was found to enhance tolerance to fenoxaprop-P-ethyl, but not to clethodim and pinoxaden. Additionally, rice calli overexpressing the <em>AmCYP71AF43</em> gene exhibited resistance to fenoxaprop-P-ethyl, but not to clethodim or pinoxaden. Collectively, the increased expression of <em>CYP71AF43</em> may enhance P450-mediated metabolism, conferring resistance to fenoxaprop-P-ethyl in the R-HB population. This is the first report of this mechanism in <em>Alopecurus myosuroides</em>. This discovery provides a novel perspective for the in-depth analysis of resistance mechanisms in weeds against the ACCase-inhibiting herbicide fenoxaprop-P-ethyl.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"209 ","pages":"Article 106355"},"PeriodicalIF":4.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into biochemical, genotoxic, and molecular impacts, and molecular docking analysis-based possible mechanism of action of some essential oils against Culex pipiens larvae","authors":"Salwa I. Sebak ,&nbsp;El-Sayed H. Shaurub ,&nbsp;Shaymaa H. Mahmoud ,&nbsp;Sherehan A.R. Salem","doi":"10.1016/j.pestbp.2025.106352","DOIUrl":"10.1016/j.pestbp.2025.106352","url":null,"abstract":"<div><div>Mosquitoes as vectors of life-threatening diseases pose significant risks to human health. While essential oils (EOs) are increasingly utilized as potential mosquitocides, their specific biochemical, genotoxic, and molecular impacts on mosquitoes are not well-documented. This study evaluates the biochemical, genotoxic, and molecular effects of five EOs on <em>Culex pipiens</em> larvae. Late third-instar larvae were treated for 24 h with LC₅₀ of orange, black pepper, camphor, lemon, and sandalwood EOs. Biochemical studies revealed that these EOs enhanced α-esterase, glutathione S-transferase, and peroxidase activities while reducing total protein, lipid, and carbohydrate contents and amylase, invertase, and trehalase activities. Acetylcholinesterase, protease, catalase, acid phosphatase, and alkaline phosphatase activities were EO-dependent. The comet assay revealed a slight increase in comet parameters, including tailed %, tail length, % DNA in the tail, tail moment, and olive tail moment, indicating low to mild DNA damage. Gene expression studies using orange and black pepper EOs demonstrated significant upregulation of genes related to immunity, detoxification, metabolism, and sensory perception. Molecular docking analysis revealed significant binding affinities between <span>d</span>-Limonene, the major component of orange oil, and its target proteins, with binding energies of −4.87, −6.33, −5.82, and − 3.38 kcal/mol for inhibitor of apoptosis protein, autophagy, juvenile hormone protein, and octopamine receptor, respectively. These findings highlight the potential of <span>d</span>-Limonene as an effective inhibitor, with favorable interactions at the receptor's active sites. This study provides insights into the possible mechanism of EO toxicity, offering promising directions for developing eco-friendly mosquito control strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"209 ","pages":"Article 106352"},"PeriodicalIF":4.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA-seq-based identification of imidacloprid resistance mechanisms in Nilaparvata lugens and development of diagnostic tools for the CYP6ER1 variant","authors":"Minyoung Choi ,&nbsp;Murtaza Khan ,&nbsp;Changhee Han ,&nbsp;In-hong Jeong ,&nbsp;Nakjung Choi ,&nbsp;Juil Kim","doi":"10.1016/j.pestbp.2025.106351","DOIUrl":"10.1016/j.pestbp.2025.106351","url":null,"abstract":"<div><div>Neonicotinoid insecticides, such as imidacloprid, have been used for over three decades to control major agricultural pests, including brown planthopper (<em>Nilaparvata lugens</em>), aphids, and other sap-feeding insects. However, the extensive use of imidacloprid has led to widespread resistance across Asia in many pest populations. Bioassay results demonstrated significant resistance in <em>N. lugens</em>, with a resistance ratio of 32. RNA-seq analysis revealed that the overexpression of detoxification enzyme genes plays a central role in resistance, with <em>CYP6ER1</em> being the most significantly upregulated gene, showing a 28-fold increase in resistant strain compared to susceptible strain. Among detoxification enzymes, carboxylesterases and glutathione-S-transferases exhibited slightly resistance-specific overexpression, while others remained unchanged. To date, six main variants of <em>CYP6ER1</em> have been reported. In the current study, <em>CYP6ER1vA</em> has been identified as the predominant variant across all resistant strains, field populations collected in 2024, and museum samples from 1975. This suggests that the vA variant predated the widespread use of imidacloprid and that its expression level, rather than its mere presence, is the key factor driving resistance. A LAMP-PCR diagnostic method was developed to detect <em>CYP6ER1vA</em>, offering a rapid and reliable tool for field-based resistance monitoring. These findings support systematic imidacloprid resistance management in <em>N. lugens</em>, integrating molecular diagnostic with sustainable pest control strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"209 ","pages":"Article 106351"},"PeriodicalIF":4.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased expression of an isoform of the long non-coding RNA, lnc37707, is associated with malathion resistance in Bactrocera dorsalis","authors":"Li-Wei Meng ,&nbsp;Zong-Yu Luo ,&nbsp;Fu-Qiang Zhang ,&nbsp;Yi-Xuan Dong ,&nbsp;Chao Ye ,&nbsp;Wei Zhang ,&nbsp;Jin-Jun Wang","doi":"10.1016/j.pestbp.2025.106343","DOIUrl":"10.1016/j.pestbp.2025.106343","url":null,"abstract":"<div><div>The oriental fruit fly, <em>Bactrocera dorsalis</em> (Hendel) is an invasive pest threatening global fruit industries. Field populations of <em>B. dorsalis</em> exhibit complex insecticide resistance, hindering pest control efforts and exacerbating damage. Long non-coding RNAs (lncRNAs) are critical regulators of multiple bioprocess in insects, including insecticide resistance, and have potentials as novel target for pest management. Here, the candidate lncRNAs associated with malathion resistance in <em>B. dorsalis</em> were identified through RNA-seq. One of the isoforms of <em>lnc37707</em>, designated as <em>lnc37707.10</em>, was significantly enriched in the detoxification tissues of malathion-resistant (MR) strain. A specific fragment of <em>lnc37707.10</em> (<em>sflnc37707</em>) was strongly associated with malathion resistance, and silencing <em>sflnc37707</em> increased the susceptibility, whereas overexpressing it decreased susceptibility to malathion. Silencing <em>sflnc37707</em> resulted in the down-regulation of 248 genes, but none of them included the four adjacent genes as its potential target. Instead, pathway analysis revealed significant enrichment of down-regulated genes involved in drug and xenobiotics metabolism, including P450s and GSTs. Bioinformatic analysis suggested a potential regulatory role of miRNA in the function of <em>lnc37707</em>. Further combining silence or overexpression <em>sflnc37707</em> with miRNA mimic treatment identified that <em>BdGSTd10</em> (an important gene involved in malathion resistance) and miR-1000 was strongly linked to <em>lnc37707.10</em>. Finally, a ceRNA (competing endogenous RNA) regulatory axis was proposed, where <em>lnc37707.10</em> might indirectly modulate <em>BdGSTd10</em> by sponging miR-1000 to regulate the malathion resistance in <em>B. dorsalis</em>. These findings provide a new insight into insecticide resistance and a potential lncRNA target for the sustainable pest management.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"209 ","pages":"Article 106343"},"PeriodicalIF":4.2,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amisulbrom induces mitochondrial dysfunction, leading apoptosis and cell cycle arrest in human trophoblast and endometrial cells","authors":"Miji Kim ,&nbsp;Wonhyoung Park ,&nbsp;Whasun Lim ,&nbsp;Gwonhwa Song ,&nbsp;Sunwoo Park","doi":"10.1016/j.pestbp.2025.106347","DOIUrl":"10.1016/j.pestbp.2025.106347","url":null,"abstract":"<div><div>Amisulbrom, a triazole-based fungicide, is utilized in agriculture to increase agricultural production by controlling fungal infections. The long disappearance time of 50 % (DT50) and potential toxic effects of amisulbrom on nontarget organisms have been reported. However, the toxic effects on the pregnancy process remain unclear. This study aims to determine the cytotoxic responses of human trophoblast cells (HTR-8/SVneo) and human endometrial cells (T HESCs), which are associated with implantation upon amisulbrom exposure. Mitochondrial dysfunction and intracellular Ca²⁺ overload were determined in both cells that are exposed to amisulbrom. Additionally, amisulbrom arrested the cell cycle progression in the G₂/M phase, causing apoptosis and reduced survival. Excessive reactive oxygen species (ROS) accumulation and dephosphorylation of PI3K/AKT signaling proteins by amisulbrom exposure mediated these toxic effects. Additionally, spheroid formation was inhibited by amisulbrom treatment in the three-dimensional hanging drop culture model. These results indicate that amisulbrom may pose an adverse effect on the implantation process. Further research is required to identify the toxicity of amisulbrom in vivo. This is the first study to raise concerns about possible toxicity mechanisms of amisulbrom in the implantation process.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"209 ","pages":"Article 106347"},"PeriodicalIF":4.2,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}