Zhiheng Zhou , Dongya Shi , Qiaowan Chen , Chang Liu , Guiping Zhang , Haijun Ma , Zhonghua Ma
{"title":"伪谷草镰刀菌ZJS-178抗性突变体的特性研究","authors":"Zhiheng Zhou , Dongya Shi , Qiaowan Chen , Chang Liu , Guiping Zhang , Haijun Ma , Zhonghua Ma","doi":"10.1016/j.pestbp.2025.106709","DOIUrl":null,"url":null,"abstract":"<div><div><em>Fusarium pseudograminearum</em> is a major causal agent of Fusarium crown rot (FCR) in wheat worldwide. ZJS-178, a cyanoacrylate fungicide and phenamacril derivative, has shown strong inhibitory activity against <em>Fusarium</em> species. However, its resistance risk and underlying resistance mechanisms in <em>F. pseudograminearum</em> remain unexplored. In this study, three sensitive <em>F. pseudograminearum</em> isolates were subjected to fungicide adaptation in the laboratory, resulting in 35 ZJS-178 resistant mutants, 22 of which exhibited high-level resistance. Compared to their parental strains, resistant mutants displayed reduced fitness, including impaired mycelial growth, conidiation, conidial germination, deoxynivalenol production, and virulence. Cross-resistance analysis revealed a positive correlation between ZJS-178 and phenamacril, but no cross-resistance with fludioxonil, tebuconazole, carbendazim, or prochloraz. Sequence alignment and gene replacement assays identified 10 point-mutations in the <em>FpMyoI</em> gene (encoding myosin I) as the genetic basis for resistance. Molecular docking analysis further confirmed that these mutations interfere with fungicide binding. This study provides critical insights into the molecular basis of ZJS-178 resistance and offers valuable guidance for resistance monitoring and management strategies in FCR control.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"216 ","pages":"Article 106709"},"PeriodicalIF":4.0000,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characteristics of ZJS-178 resistant mutants of Fusarium pseudograminearum\",\"authors\":\"Zhiheng Zhou , Dongya Shi , Qiaowan Chen , Chang Liu , Guiping Zhang , Haijun Ma , Zhonghua Ma\",\"doi\":\"10.1016/j.pestbp.2025.106709\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Fusarium pseudograminearum</em> is a major causal agent of Fusarium crown rot (FCR) in wheat worldwide. ZJS-178, a cyanoacrylate fungicide and phenamacril derivative, has shown strong inhibitory activity against <em>Fusarium</em> species. However, its resistance risk and underlying resistance mechanisms in <em>F. pseudograminearum</em> remain unexplored. In this study, three sensitive <em>F. pseudograminearum</em> isolates were subjected to fungicide adaptation in the laboratory, resulting in 35 ZJS-178 resistant mutants, 22 of which exhibited high-level resistance. Compared to their parental strains, resistant mutants displayed reduced fitness, including impaired mycelial growth, conidiation, conidial germination, deoxynivalenol production, and virulence. Cross-resistance analysis revealed a positive correlation between ZJS-178 and phenamacril, but no cross-resistance with fludioxonil, tebuconazole, carbendazim, or prochloraz. Sequence alignment and gene replacement assays identified 10 point-mutations in the <em>FpMyoI</em> gene (encoding myosin I) as the genetic basis for resistance. Molecular docking analysis further confirmed that these mutations interfere with fungicide binding. This study provides critical insights into the molecular basis of ZJS-178 resistance and offers valuable guidance for resistance monitoring and management strategies in FCR control.</div></div>\",\"PeriodicalId\":19828,\"journal\":{\"name\":\"Pesticide Biochemistry and Physiology\",\"volume\":\"216 \",\"pages\":\"Article 106709\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2025-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pesticide Biochemistry and Physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0048357525004225\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticide Biochemistry and Physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0048357525004225","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Characteristics of ZJS-178 resistant mutants of Fusarium pseudograminearum
Fusarium pseudograminearum is a major causal agent of Fusarium crown rot (FCR) in wheat worldwide. ZJS-178, a cyanoacrylate fungicide and phenamacril derivative, has shown strong inhibitory activity against Fusarium species. However, its resistance risk and underlying resistance mechanisms in F. pseudograminearum remain unexplored. In this study, three sensitive F. pseudograminearum isolates were subjected to fungicide adaptation in the laboratory, resulting in 35 ZJS-178 resistant mutants, 22 of which exhibited high-level resistance. Compared to their parental strains, resistant mutants displayed reduced fitness, including impaired mycelial growth, conidiation, conidial germination, deoxynivalenol production, and virulence. Cross-resistance analysis revealed a positive correlation between ZJS-178 and phenamacril, but no cross-resistance with fludioxonil, tebuconazole, carbendazim, or prochloraz. Sequence alignment and gene replacement assays identified 10 point-mutations in the FpMyoI gene (encoding myosin I) as the genetic basis for resistance. Molecular docking analysis further confirmed that these mutations interfere with fungicide binding. This study provides critical insights into the molecular basis of ZJS-178 resistance and offers valuable guidance for resistance monitoring and management strategies in FCR control.
期刊介绍:
Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance.
Research Areas Emphasized Include the Biochemistry and Physiology of:
• Comparative toxicity
• Mode of action
• Pathophysiology
• Plant growth regulators
• Resistance
• Other effects of pesticides on both parasites and hosts.