Pesticide Biochemistry and Physiology最新文献

{"title":"The role of 20-hydroxyecdysone and juvenile hormone in insecticidal activity of Bacillus thuringiensis regulated by DUOX-ROS immunity in Spodoptera exigua","authors":"Yujie Ji ,&nbsp;Bo Gao ,&nbsp;Dan Zhao ,&nbsp;Lu Zhang ,&nbsp;Han Wu ,&nbsp;Yifan Xie ,&nbsp;Qiuyu Shi ,&nbsp;Yao Wang ,&nbsp;Wei Guo","doi":"10.1016/j.pestbp.2024.106222","DOIUrl":"10.1016/j.pestbp.2024.106222","url":null,"abstract":"<div><div>Insect midgut bacteria can be transferred to the blood cavity due to Bt infection and proliferate, becoming pathogens and enhancing Bt insecticidal activity. Dual oxidase (DUOX)-reactive oxygen species (ROS) signaling pathway plays a key role in regulating microbial homeostasis and resisting pathogen infection. However, the functions of MEKK and MKK associated with DUOX-ROS immunity are rarely studied in insects, moreover, the regulatory mechanisms underlying DUOX-ROS immunity via 20-Hydroxyecdysone (20E) and juvenile hormone (JH) are underexplored. In this study, we investigated that <em>Spodoptera exigua</em> MAPK kinase kinase 4 (<em>SeMEKK4</em>) and MAPK kinase 6 (<em>SeMKK6</em>) were required for <em>Sep38β</em> expression, and RNAi-mediated knockdown of <em>SeMEKK4</em> and <em>SeMKK6</em> significantly decreased ROS level and increased bacterial load in the midgut of <em>S. exigua</em> larvae, thereby enhancing Bt insecticidal activity. Furthermore, 20E and JH titers were elevated in insects infected with Bt. 20E <u>u</u>pregulated the expression of <em>SeMEKK4</em>, <em>SeMKK6</em>, and <em>Sep38β</em> through <em>SeEcR</em> and <em>SeUSP</em> receptors, and activated the expression of <em>SeDUOX</em> to increase ROS level and decrease bacterial load in the midgut, which was not conducive to the enhancement of Bt insecticide activity. JH showed an opposite effect on midgut-related DUOX-ROS immunity via <em>SeMet1</em> and <em>SeMet2</em>, and it was noteworthy that JH played a dominant role in negatively regulating DUOX-ROS immunity post Bt infection, which enhanced Bt insecticidal activity. This is an adjustment strategy for insects to cope with Bt infection, providing a new perspective for pest management.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106222"},"PeriodicalIF":4.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silencing OdfoP-gp1 and OdfoP-gp3 with dsRNA increased the lethality of the nonsteroidal ecdysone agonist RH-5849 against Odontotermes formosanus (Shiraki)","authors":"Yan Xiong ,&nbsp;Lingyu Xie ,&nbsp;Fang Tang","doi":"10.1016/j.pestbp.2024.106226","DOIUrl":"10.1016/j.pestbp.2024.106226","url":null,"abstract":"<div><div><em>Odontotermes formosanus</em> (Shiraki) is a highly damaging social insect that causes significant economic losses. Insect growth regulators (IGRs) are efficient and environmentally friendly insecticides. 1,2-dibenzoyl-1-tert-butylhydrazine (RH-5849), a nonsteroidal ecdysone agonist of IGR, is highly effective in controlling various insect species. In this study, the toxicity of RH-5849 against <em>O. formosanus</em> was assessed. To investigate whether ATP-binding cassette (ABC) transporter proteins, detoxification enzymes, play crucial roles in the defense of <em>O. formosanus</em> against RH-5849, transcriptome sequencing was conducted in <em>O. formosanus</em>. Through RNA sequencing, 27 ABC transporter protein genes were identified, 23 of which were significantly upregulated after <em>O. formosanus</em> was exposed to RH-5849, especially two <em>P-glycoprotein</em> genes, <em>OdfoP-gp1</em> and <em>OdfoP-gp3</em>, whose expression levels were the most significantly upregulated, at 5.79 and 5.56, respectively. Furthermore, using dsRNAs to interfere with the expression of <em>OdfoP-gp1</em> and <em>OdfoP-gp3</em>, we found that the maximum interference efficiency was achieved at 24 h, with efficiencies of 85.30 % and 59.59 %, respectively. Based on these findings, dsRNA was coupled with RH-5849, and the results indicated that the lethality of RH-5849 against <em>O. formosanus</em> significantly increased following dsRNA interference. Overall, the lethality of RH-5849 significantly increased following dsRNA interference, suggesting that ABC transporter proteins, especially P-glycoproteins, play a crucial role in the defense of <em>O. formosanus</em> against RH-5849. This study provides a new strategy for termite control through the combination of dsRNA and IGR.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106226"},"PeriodicalIF":4.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol activity and action mechanism of Pseudomonas aurantiaca ST-TJ4 against Verticillium dahliae, the causal agent of Acer truncatum wilt","authors":"Liang-Liang Hou ,&nbsp;Wei-Liang Kong ,&nbsp;Xiao-Qin Wu","doi":"10.1016/j.pestbp.2024.106224","DOIUrl":"10.1016/j.pestbp.2024.106224","url":null,"abstract":"<div><div><em>Acer truncatum</em> wilt caused by <em>Verticillium dahliae</em> is a severe soilborne disease that poses a threat to the cultivation of this plant in China. The present study explored the biocontrol efficiency and underlying antagonistic mechanism of <em>Pseudomonas aurantiaca</em> ST-TJ4 against <em>V. dahliae</em>. In vitro, strain ST-TJ4 exhibited excellent inhibitory effects on <em>V. dahliae</em>, causing mycelial deformation. This strain significantly suppressed the production of <em>V. dahliae</em> conidia and microsclerotia<em>.</em> Moreover, the application of ST-TJ4 reduced the incidence of <em>Verticillium</em> wilt in <em>A. truncatum</em> saplings in both the prevention group and the cure group. Comparative transcriptomic analyses revealed that ST-TJ4 induced differential expression of numerous genes in <em>V. dahliae</em>, most of which were downregulated. These differentially expressed genes were associated with cell wall-degrading enzyme activity, sterol biosynthetic processes, glutathione S-transferase activity, iron ion and sugar metabolism, and oxidoreductase activity. Further transcriptomic analyses of physiological indices indicated that ST-TJ4 significantly inhibited the synthesis of pectin lyase, <em>endo</em>-β-1,4-glucanase, melanin and soluble sugars of <em>V. dahliae</em> and had a stronger inhibitory effect under iron deficiency. Taken together, these data highlight <em>P. aurantiaca</em> ST-TJ4 as a promising biocontrol agent against <em>A. truncatum</em> Verticillium wilt.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106224"},"PeriodicalIF":4.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C-type lectin 9 participates in the immune response, development and reproduction of Tribolium castaneum","authors":"Chengjun Li,&nbsp;Huayi Ai,&nbsp;Biao Zhang,&nbsp;Xiaoqiao Huang,&nbsp;Bin Li","doi":"10.1016/j.pestbp.2024.106223","DOIUrl":"10.1016/j.pestbp.2024.106223","url":null,"abstract":"<div><div>C-type lectins (CTLs), as a large family of pattern recognition receptors (PRRs), have been reported to be involved in bacterial infection, but the role of CTLs in development has been poorly understood in insects. The orthologues of <em>Tribolium castaneum</em> CTL9 (TcCTL9) have been identified among insects, but its functions were currently unclear. Therefore, we performed functional analysis of TcCTL9 in this study. Our results indicated that TcCTL9 could bind to bacteria through lipopolysaccharide and peptidoglycan, and agglutinate Gram-positive and Gram-positive bacteria in a Ca²⁺-dependent manner. Silencing <em>TcCTL9</em> reduced the immune resistance to <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>, decreased the expression of antimicrobial peptides and prophenoloxidase, and inhibited the phenoloxidase activity. These data suggested that TcCTL9 functioned in the immune response via the Toll and IMD pathways and prophenoloxidase system. During development, <em>TcCTL9</em> had high expression in the periods of egg to larva and pupa to adult, and knockdown of <em>TcCTL9</em> suppressed the metamorphosis, egg production and hatchability, and ovary development through ecdysone and juvenile hormone pathways in <em>T. castaneum</em>. This study comprehensively clarified the functions of TcCTL9 orthologues in insects and provided the theoretical basis for developing novel targets of pesticides.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106223"},"PeriodicalIF":4.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The function of HgLac in Heterodera glycines and its potential as a control target","authors":"Dongya Wang ,&nbsp;Fangcao Wu ,&nbsp;Xiongbiao Xu ,&nbsp;Deliang Peng ,&nbsp;Yuxi Duan ,&nbsp;Huan Peng ,&nbsp;Haiyan Wu","doi":"10.1016/j.pestbp.2024.106225","DOIUrl":"10.1016/j.pestbp.2024.106225","url":null,"abstract":"<div><div>The soybean cyst nematode (SCN; <em>Heterodera glycines</em>) is one of the most devastating pathogens for soybean production. The second stage juvenile (J2) invades the host root, develops and form white females which then become brown cysts enter the soil. The brown cyst wall plays a key role in protecting inside eggs from adverse environmental conditions. However, the function of cyst wall tanning (sclerotization and pigmentation) in nematodes is not clear. A browning-related gene discovered from the whole-genome sequencing was cloned and characterized in this study, the gene was confirmed to be the laccase gene and was named <em>HgLac</em>. <em>HgLac</em> mRNA and HgLac protein was detected in the epidermis of juveniles using <em>in situ</em> hybridization and immunolocalization techniques. The <em>HgLac</em> expression level was greater in fourth-stage juveniles (J4s) than in the other stages. Knockdown of <em>HgLac</em> by <em>in vitro</em> RNA interference (RNAi) significantly decreased the infectivity, development and reproduction of J2s but had no effect on cyst wall tanning. Further research revealed that <em>HgLac</em> expression in nematodes was significantly suppressed by 35.41–59.17 % through <em>in planta</em> RNAi, 52.96–58.19 % females could not tan successfully, and the female wall was very soft and fragile, with a low egg hatching rate (1.33 %), which was significantly lower than that of normal females (68.85 %). These results indicate that <em>HgLac</em> plays a key role in cyst wall tanning and suppressing the development and reproduction of the SCN, which provides new ideas for the use of this gene as a target to control SCN.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106225"},"PeriodicalIF":4.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin E alleviates zebrafish intestinal damage and microbial disturbances caused by pyraclostrobin","authors":"Mengna Zhang ,&nbsp;Feng Zhao ,&nbsp;Mengyu Guo ,&nbsp;Manman Duan ,&nbsp;Yao Xie ,&nbsp;Lihong Qiu","doi":"10.1016/j.pestbp.2024.106221","DOIUrl":"10.1016/j.pestbp.2024.106221","url":null,"abstract":"<div><div>Pyraclostrobin (PY) is highly toxic to aquatic organisms, and its increased residues in aquatic environments may have harmful effects on the intestine of aquatic creatures. Previous research demonstrated that vitamin E (VE) alleviated the acute toxicity of PY to zebrafish. This study further explored the mitigation effect of VE on PY-induced intestinal toxicity in fish and the underlying mechanisms by exposing adult zebrafish to PY (10, 20 μg/L) with or without 4 μM VE supplementation for 21 days. The results showed that VE alleviated the gut histopathological lesions caused by PY. VE co-exposure also improved PY-induced intestinal inflammation and restored the expression level of genes encoding intestinal tight junction protein. Furthermore, VE restored the anti-oxidation level inhibited by PY and reduced pro-apoptotic cytokine level and apoptotic enzyme activity increased by PY. 16S rRNA high-throughput sequencing showed that VE improved the zebrafish intestinal flora imbalance caused by 20 μg/L PY, increased the relative abundance of beneficial bacterium <em>Cetobacterium</em>, and reduced the relative abundance of pathogenic bacteria. In conclusion, VE alleviated PY-induced intestinal toxicity via repairing the damaged intestinal mucosal barrier, inhibiting inflammation, reducing oxidative stress and apoptosis, and improving the intestinal microbial disorder in zebrafish.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106221"},"PeriodicalIF":4.2,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of the transfluthrin effects on fertility and pregnancy outcomes: An in-vivo study in rat","authors":"Akhilesh Mishra ,&nbsp;Gayatri Dewangan ,&nbsp;Megh Singh Dhakad ,&nbsp;Subash Chandra Sonkar ,&nbsp;Jasbir Dalal ,&nbsp;Sunita Pradhan ,&nbsp;Divakar Sharma ,&nbsp;Vandana Roy ,&nbsp;Bidhan Chandra Koner","doi":"10.1016/j.pestbp.2024.106220","DOIUrl":"10.1016/j.pestbp.2024.106220","url":null,"abstract":"<div><div>Transfluthrin (TFL) is a rapid-acting pyrethroid insecticide, commonly used worldwide, however its overuse and or misuse has been associated with adverse effects on human health. This study was designed to investigate the impact of TFL on fertility and pregnancy in Sprague–Dawley rats exposed orally and by inhalation. In this study, 48 adult Sprague–Dawley rats of 6–8 months age group were randomly selected with equal numbers of males and females. Each group was housed separately in a standard laboratory condition and their daily consumption of food and water, along with weight of each group were monitored. The blood was collected from each rat on 0 (zero) and 85th day of oral and inhalation exposure to TFL to determine the hormone levels by ELISA. The findings revealed that TFL exposure through oral and inhalation route reduced hormones level in serum such as testosterone, follicle-stimulating hormone, luteinizing hormone, progesterone, estrogen, and prolactin in both male and female rats. In conclusion, current research suggest the negative effects of TFL exposure on fertility, adversely affecting pregnancy outcomes, reducing the mating rate, and lowering the number of pups. It also stresses the significance of controlling its use to prevent any negative consequences.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106220"},"PeriodicalIF":4.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of the β-N-acetylhexosaminidase genes by RNA interference inhibited the molting and increased the mortality of the white-backed planthopper, Sogatella furcifera","authors":"Pan-Pan Guo ,&nbsp;Xi-Bin Yang ,&nbsp;Hong Yang ,&nbsp;Cao Zhou ,&nbsp;Gui-Yun Long ,&nbsp;Dao-Chao Jin","doi":"10.1016/j.pestbp.2024.106216","DOIUrl":"10.1016/j.pestbp.2024.106216","url":null,"abstract":"<div><div><em>β</em>-N-Acetylglucosaminidases and/or <em>β</em>-<em>N</em>-acetylhexosaminidases (NAGs / Hexes) are crucial exonucleases, playing a crucial role in the insect molting process. SfHex3 and SfHex4 contain conserved catalytic domains of GH20 and GH20b, clustered into NAG2 and NAG1 group, respectively. <em>SfHex3</em> and <em>SfHex4</em> were mainly highly expressed in the 4th–5th instar nymphs, as well as in the integument and ovary. The expression level of <em>SfHex3</em> gradually decreased in male and female adults, and <em>SfHex4</em> on the first day of female was significantly higher than that on the first day of male. In addition, RNA interference (RNAi) results demonstrated that the downregulation of <em>SfHex3</em> and <em>SfHex4</em> expression in 5th-instar nymphs resulted in failed molting, and a high mortality. Furthermore, after RNAi with <em>SfHex3</em> and <em>SfHex4</em>, the transcript levels on key genes of the chitin metabolism pathway (<em>SfCHS1</em>, <em>SfCHS1a</em>, <em>SfCHS1b</em>, <em>SfTRE1</em>, <em>SfTRE2</em>, <em>SfCht5</em>, and <em>SfCht7</em>) were significantly decreased compared to the control group. Meanwhile the expression levels of <em>SfHex3</em> and <em>SfHex4</em> were up-regulated after 6 h and 12 h of 20E treatment. And the transcription levels of <em>SfHex3</em> and <em>SfHex4</em> were significantly inhibited at nitenpyram LC₂₀, LC₅₀, and LC₉₀ after 96 h of treatment, in 3rd nymphs of <em>Sogatella furcifera</em>. In conclusion, <em>SfHex3</em> and <em>SfHex4</em> play important roles in the nymphal development of <em>S. furcifera</em>, contributing to the molting process from nymph to adult. This study not only enhances our understanding of the nitenpyram in pest control, but also provides a foundation for the development of new control strategies using RNAi to targeting <em>SfHex3</em> and <em>SfHex4</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106216"},"PeriodicalIF":4.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution, frequency and molecular basis of penoxsulam, metamifop and florpyrauxifen-benzyl resistance in Echinocloa spp. from rice fields across Jiangsu Province, China","authors":"Wei Deng ,&nbsp;Hanqi Yin ,&nbsp;Zhixun Ge ,&nbsp;Sai Yao ,&nbsp;Jiawen Wu ,&nbsp;Axiu Zhu ,&nbsp;Qian Yang ,&nbsp;Shuzhong Yuan","doi":"10.1016/j.pestbp.2024.106218","DOIUrl":"10.1016/j.pestbp.2024.106218","url":null,"abstract":"<div><div>Barnyard grasses (<em>Echinochloa</em> spp.) are the most troublesome weeds in rice ecosystems of Jiangsu Province, China, and long-term use of herbicides inevitably cause the development of resistance. In this study, 122 field-sampled populations were tested for resistance to penoxsulam (PEN), metamifop (MET), florpyrauxifen-benzyl, (FPB), propanil, and tripyrasulfone. Field-dose assays revealed that 51, 7, and 6 populations have either developed or were developing resistance to penoxsulam, metamifop and florpyrauxifen-benzyl, and all 122 populations were still sensitive to propanil and tripyrasulfone. Dose-response assays identified that 41.8 % of populations exhibited 6- to 830-fold resistance to PEN; 5.7 % of populations displayed 2.2- to 13.9-fold resistance to MET; and 4.9 % of populations showed 2.4- to 11.1-fold resistance to FPB. Trp-574-Leu mutations in <em>EcALS1 or EcALS3</em> were found in 18.0 % of populations. Trp-2027-Cys, Cys-2088-Arg, and Gly-2096-Ala mutations in <em>EcACC1, EcACC3,</em> or <em>EcACC5</em> were detected in 4.1 % of populations, and no known resistance-associated target-site gene mutations were confirmed in the rest of herbicide-resistant populations. Furthermore, the P450 inhibitor malathion and GST inhibitor NBD-Cl significantly reduced the resistance to three herbicides in 38.5 % of populations, especially those without the target-site gene mutations, indicating both target-site mutations and P450s and/or GSTs-mediated metabolisms play important roles in herbicide resistance evolution in <em>Echinochloa</em> spp. These findings are helpful to formulate an appropriate strategy for effective and sustainable control of <em>Echinochloa</em> spp. by mixtures of herbicide with different modes of action and mitigate weed resistance evolution.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"207 ","pages":"Article 106218"},"PeriodicalIF":4.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between imidacloprid residues in maize rhizospheric soil and soil nematode community","authors":"Jiaming Zhang ,&nbsp;Yue Wu ,&nbsp;Jiayi Liu,&nbsp;Xinru Wang,&nbsp;Weijia Pang,&nbsp;Juan Hua,&nbsp;Shihong Luo","doi":"10.1016/j.pestbp.2024.106219","DOIUrl":"10.1016/j.pestbp.2024.106219","url":null,"abstract":"<div><div>Although imidacloprid has been shown to present potential risks to non-target invertebrates and vertebrates, researches exploring this risk from the perspective of the underground ecosystem remains incomplete. In this study, we determined that the presence of imidacloprid significantly reduced the abundance and diversity of soil nematodes in maize rhizospheric soil. Furthermore, imidacloprid also exerted negative effects on the body length, reproduction, locomotion, lipid accumulation, lipofuscin accumulation, and acetylcholinesterase activity in the model organism <em>Caenorhabditis elegans</em>. These toxic phenotypes are correlated with the upregulation of <em>fat-2</em>, <em>fat-6</em>, <em>hsp-16.41</em>, and <em>hsp-16.2</em>, along with the downregulation of <em>ace-1</em>, <em>ace-2</em>, and <em>ace-3</em>. In response to these toxic effects of imidacloprid, nematodes also developed corresponding adaptive mechanisms. UPLC-MS/MS analysis revealed that nematodes could convert imidacloprid to imidacloprid-guanidine and imidacloprid-urea to reduce the toxicity of imidacloprid. Moreover, <em>C. elegans</em> and <em>Meloidogyne incognita</em> exhibited repellent behavior towards imidacloprid-treated area, even the concentration of imidacloprid is only 0.4 μg/mL. This study revealed the interaction between imidacloprid and nematodes, providing a basis for understanding the potential risks of non-target soil nematodes after application of imidacloprid in sustainable agriculture and the resistance mechanism of nematodes to nematocidal pesticide.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106219"},"PeriodicalIF":4.2,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}