Pesticide Biochemistry and Physiology最新文献

{"title":"Role of Bacillus atrophaeus B1 in gut on nicotine tolerance of the fall armyworm","authors":"Yaping Chen ,&nbsp;Wenyuan Zhao ,&nbsp;Yanfang Hu ,&nbsp;Yao Chen ,&nbsp;Yahong Li ,&nbsp;Zhongxiang Sun ,&nbsp;Yuerui Li ,&nbsp;Rongchao Luo ,&nbsp;Ewei Du ,&nbsp;Furong Gui","doi":"10.1016/j.pestbp.2024.106248","DOIUrl":"10.1016/j.pestbp.2024.106248","url":null,"abstract":"<div><div>The fall armyworm (FAW), <em>Spodoptera frugiperda</em> is one of the most destructive polyphagous herbivores. Some detoxification genes have been proved to be involved in the adaptability to host plants in FAW, while the role of its gut microbiota on the responses of host switches, and their ability to adapt to new host plants remain poorly understood. Herein, we isolated five strains of nicotine-degrading bacteria from the gut of <em>S. frugiperda</em> larvae, among which <em>Bacillus atrophaeus</em> B1 exhibited the highest nicotine tolerance. This strain showed a minimum inhibitory concentration (MIC) value of 2 g/L and a nicotine degradation rate of 46.36 %. We sequenced the complete genome of <em>B. atrophaeus</em> B1 and 15 candidate genes were identified maybe related to nicotine degradation, among which <em>GE003027</em>, <em>GE002849</em>, <em>GE002602, GE000220</em> and <em>GE002708</em> had significantly higher expression when exposed to nicotine. Non-targeted metabolomics revealed 98 differentially accumulated metabolites (DAMs) under nicotine stress, which were 72 metabolites upregulated and 26 metabolites downregulated, and the pathways most affected involved xenobiotic biodegradation and metabolism, energy metabolism, and amino acid metabolism. <em>B. atrophaeus</em> B1 may accumulate 2-ketoglutaric acid and γ-aminobutyric acid during degradation of nicotine, which is non-toxic to <em>S. frugiperda</em>, and participated in the tricarboxylic acid (TCA) cycle. Additionally, 2-ketoglutaric acid and γ-aminobutyric acid were detected both in <em>B. atrophaeus</em> B1 and <em>S. frugiperda</em> treated with nicotine. Antibiotic treatment deprived most of the gut bacteria, followed by a decrease in tolerance of <em>S. frugiperda</em> to nicotine, and the nicotine degradation rate was significantly increased as expected after reinfection with <em>B. atrophaeus</em> B1. These findings provide new insights into the bacterial metabolism of nicotine degradation and offer a theoretical basis for understanding the rapid adaptability of <em>S. frugiperda</em> to various host plants.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106248"},"PeriodicalIF":4.2,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk assessment of broflanilide resistance in Panonychus citri (McGregor): Cross-resistance, inheritance and relative fitness","authors":"Ke Li ,&nbsp;Chunyu Zhang ,&nbsp;Yiting Ren ,&nbsp;Xunyan Liu ,&nbsp;Deng Pan ,&nbsp;Wei Dou ,&nbsp;Jin-Jun Wang ,&nbsp;Guorui Yuan","doi":"10.1016/j.pestbp.2024.106256","DOIUrl":"10.1016/j.pestbp.2024.106256","url":null,"abstract":"<div><div><em>Panonychus citri</em> (McGregor) is an important economic pest in the orange orchard of the world, which has developed varying degrees of resistance to many acaricides. Broflanilide is a novel <em>γ</em>-aminobutyric acid (GABA) receptor allosteric modulator with high insecticidal activity against a broad spectrum of insects. However, the risk of resistance to broflanilide in <em>P. citri</em> has not been studied. In this study, the BR strain selected from susceptible strain of <em>P. citri</em> with broflanilide for 44 generations, developed 32.5-fold resistance to broflanilide, and did not exhibit cross-resistance to fipronil, fluxametamide, abamectin, pyridaben, and cyflumetofen. Broflanilide resistance in the BR strain of <em>P. citri</em> was autosomal, incomplete dominant and polygenic. The duration of larval and deutonymph, total life span and the number of eggs were significantly increased in the BR strain compared to the SS strain. The higher relative fitness (<em>R</em>_<em>f</em>) value (1.289) indicated that there was a fitness advantage in the BR strain. Glutathione <em>S</em>-transferase and esterase activities in the BR strain were significantly increased compared with the SS strain. These findings provide valuable information for developing resistance management strategies to delay broflanilide resistance and maintain sustainable control of <em>P. citri</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106256"},"PeriodicalIF":4.2,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143095542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flusulfinam, a novel amide herbicide to control weed in rice fields, targets 4-hydroxyphenylpyruvate dioxygenase","authors":"Hengzhi Wang ,&nbsp;He Sun ,&nbsp;Shuo Yu ,&nbsp;Lei Lian ,&nbsp;Tao Jin ,&nbsp;Xuegang Peng ,&nbsp;Jinxin Wang ,&nbsp;Weitang Liu","doi":"10.1016/j.pestbp.2024.106240","DOIUrl":"10.1016/j.pestbp.2024.106240","url":null,"abstract":"<div><div>Flusulfinam, a post-emergence (POST)-applied herbicide in rice fields to manage annual weeds, has been proven to be safe for various rice strains of japonica and indica. The study confirmed its mechanism of action by inhibiting 4-hydroxyphenylpyruvate dioxygenase (HPPD). The POST application of flusulfinam led to noticeably bleaching symptom in leaves of <em>Echinochloa crus-galli</em> within 3 to 7 days and plant mortality by 10 days. In <em>Echinochloa crus-galli</em>, flusulfinam induced a significant rise in level of phytoene content, while there was a significant decrease in levels of carotenoid and chlorophyll contents. Meanwhile, the increase in levels of chlorophyll content in <em>Spirodela polyrrhiza</em> treated with flusulfinam after homogentisic acid (HGA) addition was observed, suggesting that flusulfinam inhibited HGA production, likely by suppressing HPPD activity. Flusulfinam significantly diminished the catalytic activity of recombinant <em>Arabidopsis thaliana</em> HPPD that was expressed by <em>Escherichia coli</em>, exhibiting an inhibitory efficacy approximately 16-fold higher than the HPPD-inhibiting mesotrione. Additionally, the rice with overexpression of <em>Oryza sativa</em> HPPD showed higher tolerance to flusulfinam than rice of wild type. Furthermore, molecular docking analyses revealed that flusulfinam formed effective bonds with the HPPD active site via the nitrogen atom of the oxadiazole ring and the oxygen atom of the amide group, with distances of 2.0 Å and 2.4 Årespectively, which engaged in bidentate coordination with the Fe²⁺ ion, with a binding energy of −8.7 kcal mol⁻¹, and HPPD-flusulfinam complex showed low root-mean square deviation values of less than 2 Å in molecular dynamics tests. This study provides the first evidence of the molecular targets of flusulfinam.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106240"},"PeriodicalIF":4.2,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA interference-mediated silencing of GNBP2 reduces the immunity of stored pest Tribolium castaneum against bacteria","authors":"Jingxiu Bi ,&nbsp;Xiangjun Ma ,&nbsp;Yuying Jiang ,&nbsp;Pingxiang Liu ,&nbsp;Rui Gao ,&nbsp;Tong Zhao ,&nbsp;Xuexia Yuan ,&nbsp;Haining Hao ,&nbsp;Bin Li ,&nbsp;Yutao Wang","doi":"10.1016/j.pestbp.2024.106230","DOIUrl":"10.1016/j.pestbp.2024.106230","url":null,"abstract":"<div><div>Gram-negative bacteria binding proteins (GNBPs) are involved in regulating the immune response of insects. The information on functions and mechanisms of insect GNBPs in innate immunity will contribute to biological control of pests. <em>Tribolium castaneum</em> is a serious, world-wide pest damaging stored food and feed products. However, the study on roles of GNBPs in <em>T. castaneum</em> innate immunity is relatively scarce. In this research, we identified <em>TcGNBP2</em>, a <em>GNBP2</em> found in the cDNA library of <em>T. castaneum</em>. Spatiotemporal examination indicated that <em>TcGNBP2</em> exhibited significant transcription in early pupae stages, and mainly distributed in two immune-related tissues, hemolymph and fatbody. After <em>Escherichia coli</em> or <em>Staphylococcus aureus</em> challenge, <em>TcGNBP2</em> transcription levels increased significantly from 6 to 72 h. The binding ability of TcGNBP2 to lipopolysaccharide, peptidoglycan, and β-1,3-glucan was predicted by molecular docking analysis and confirmed by ELISA. The subsequent investigation revealed that TcGNBP2 exhibited binding affinity towards five distinct bacterial strains and demonstrated agglutination activity against four of them. Silencing of <em>TcGNBP2</em> with RNA interference (RNAi) results in the inhibition of antimicrobial peptide gene expression and the prophenoloxidase cascade in beetles upon bacterial challenge, thereby attenuating the immune response of <em>T. castaneum</em>. The survival tests revealed that the knockdown of <em>TcGNBP2</em> significantly compromised <em>T. castaneum</em>'s resistance to bacterial infection. Our findings provide valuable insights into the regulatory mechanism of TcGNBP2 in the innate immunity of <em>T. castaneum</em> and offer a promising molecular target for RNAi-based management of insect pest.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106230"},"PeriodicalIF":4.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential pathogenicity of heterologous nucleopolyhedroviruses on Spodoptera frugiperda corresponds to modulated expression of immune-response-related genes","authors":"Yusheng Wang ,&nbsp;Zihan Shi ,&nbsp;Shaolong Wu ,&nbsp;Weiai Zeng ,&nbsp;Can Wang ,&nbsp;Yingying Zhao ,&nbsp;Rujing Deng ,&nbsp;Rui Shi ,&nbsp;Yansong Xiao ,&nbsp;Lin Tan","doi":"10.1016/j.pestbp.2024.106232","DOIUrl":"10.1016/j.pestbp.2024.106232","url":null,"abstract":"<div><div>The fall armyworm <em>Spodoptera frugiperda</em> (J.E. Smith) (Lepidoptera: Noctuidae) is a notorious invasive pest wreaking havoc on various crops globally. Nucleopolyhedroviruses (NPVs) are viral pathogens that specially target lepidopteran pests. However, the homologous virus, <em>Spodoptera frugiperda</em> multiple nucleopolyhedrovirus (SfMNPV), has not been commercialized in China. Therefore, understanding the molecular mechanisms underlying heterologous virus-host interactions can inform the design of virus-based insecticides for controlling <em>S. frugiperda</em>. The pathogenicity of the four heterologous NPVs on <em>S. frugiperda</em> varied greatly. Mamestra brassicae multiple nucleopolyhedrovirus (MbMNPV) exhibited the most potent virulence on larvae and induced the most robust sublethal effects on adults. <em>Spodoptera exigua</em> multiple nucleopolyhedrovirus (SeMNPV) infection was characterized by more moderate pathogenicity, and larvae were relatively resistant to Helicoverpa armigera single nucleopolyhedrovirus (HaSNPV) and <em>Spodoptera litura</em> multiple nucleopolyhedrovirus (SlMNPV). Larval mortality was virus-concentration and larval stage dependent. Specifically, the corrected mortality rate of third instar larvae after treatment with 1 × 10⁶, 1 × 10⁷, and 1 × 10⁸ OBs/mL MbMNPV was 88.9 %, 100.0 %, and 100.0 %, respectively. All four NPVs negatively affected the longevity and fecundity of <em>S. frugiperda</em> adults. Female adults surviving treatment with MbMNPV and SeMNPV were unable to lay eggs. Transcriptomic analysis revealed that MbMNPV infection might suppress the antiviral immune response, and dysregulate biological pathways of <em>S. frugiperda</em> larvae to facilitate systemic infection. However, the overall transcript profiles remain unchanged after SlMNPV infection. The results reinforce the potential of NPVs, specifically MbMNPV, as potent biocontrol agents for <em>S. frugiperda</em>. These findings yield valuable insights into the complex arms race between <em>S. frugiperda</em> and NPVs that may advance the development of virus-based strategies to mitigate the destructive impact of this pest.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106232"},"PeriodicalIF":4.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9–mediated editing of the melanization gene ebony in the 28-spotted ladybeetle, Henosepilachna vigintioctopunctata","authors":"Shimin Chen ,&nbsp;Yalin Ruan ,&nbsp;Zhaoyang Li ,&nbsp;Chuqiao Zhou ,&nbsp;Qi Chen ,&nbsp;Xuguo Zhou ,&nbsp;Youjun Zhang ,&nbsp;Chunxiao Yang ,&nbsp;Huipeng Pan","doi":"10.1016/j.pestbp.2024.106231","DOIUrl":"10.1016/j.pestbp.2024.106231","url":null,"abstract":"<div><div>The melanization process, which is essential for the proper functioning of the cuticle, has been extensively investigated for its enzymatic roles and physiological effects. <em>Henosepilachna vigintioctopunctata</em>, a significant pest species, presents considerable economic threats. However, due to the variable efficiency of RNA interference for genetic manipulation, establishing a CRISPR/Cas9 system is crucial for providing a more precise and reliable method for functional genomics in this non-model insect. In this study, we first utilized RNAi to investigate <em>Hvebony</em>, which encodes N-β-alanyldopamine, a critical compound in cuticle melanization. Subsequently, we introduced CRISPR/Cas9 for the first time in <em>H. vigintioctopunctata</em>. RNAi experiments revealed that knockdown of <em>Hvebony</em> resulted in abnormal melanin accumulation and low mortality rates, indicating its involvement in cuticle tanning. A novel CRISPR/Cas9 workflow was established, successfully resulting in the knocking out of <em>Hvebony</em> and the creation of a stable mutant strain characterized by dark pigmentation and low fitness costs. This study establishes <em>Hvebony</em> as a promising molecular marker for genetic studies in <em>H. vigintioctopunctata</em>. Moreover, it can be utilized in the development of genome editing control strategies and for analyses of gene function in <em>H. vigintioctopunctata</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106231"},"PeriodicalIF":4.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic insights on impaired survival and enhanced pesticide susceptibility following knockdown of Syntaxin5 in Locusta migratoria","authors":"Xuanyu Chen ,&nbsp;Guangmin Wang ,&nbsp;Lian-Sheng Zang ,&nbsp;Asad Ali ,&nbsp;Natraj Krishnan ,&nbsp;Jorge R. Paredes-Montero ,&nbsp;Wei Zhang ,&nbsp;Nemat O. Keyhani ,&nbsp;Amr Mohamed","doi":"10.1016/j.pestbp.2024.106227","DOIUrl":"10.1016/j.pestbp.2024.106227","url":null,"abstract":"<div><div>Membrane fusion, essential for a variety of biological processes, is ubiquitous and critical in all living organisms. The soluble <em>N</em>-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) comprise the core machinery that mediates membrane fusion. <em>Syntaxin5</em> (Stx5) in mammals and its ortholog <em>Sed5p</em> in yeast are SNARE proteins that have been reported to mediate anterograde and retrograde endoplasmic reticulum (ER)-Golgi trafficking. In this study, a <em>syntaxin5</em> gene (<em>Lmsyx5</em>) was cloned from the migratory locust, <em>Locusta migratoria</em>, and its tissue expression pattern showed widespread expression in different tissues. Knockdown of this gene using RNA interference (RNAi) revealed that both 5^th instars and adult locusts have reduced survival, and almost all of the 5^th instar locusts died before molting. Transcriptomic analysis revealed that drug or xenobiotic metabolism-related pathways are the most significantly downregulated in the central nervous system (CNS) of the RNAi 5^th instar locusts, whereas amino acid metabolism-related pathways are significantly downregulated in the muscle. In addition, receptor interaction-related pathways are downregulated in both the CNS and muscle. The mortality rate was significantly higher when dsLmsyx5 and beta-cypermethrin were combined together than when RNAi and chemical pesticides were used alone. Taken together, the <em>Lmsyx5</em> gene is essential for locust survival, affects locust molting, is involved in the metabolism of both xenobiotics and endogenous chemicals in various tissues, and regulates locust susceptibility to chemical pesticides. It can thus be a potential target for locust control, and its knockdown using RNAi has great potential when combined with chemical pesticides.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106227"},"PeriodicalIF":4.2,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the resistance risk and resistance mechanism of florylpicoxamid in Corynespora cassiicola from cucumber","authors":"Qin Peng ,&nbsp;Xinchang Hao ,&nbsp;Chunyue Liu ,&nbsp;Xiuhuan Li ,&nbsp;Xingxing Lu ,&nbsp;Xili Liu","doi":"10.1016/j.pestbp.2024.106228","DOIUrl":"10.1016/j.pestbp.2024.106228","url":null,"abstract":"<div><div>Florylpicoxamid, a QiI fungicide, demonstrates broad-spectrum activity against a wide range of phytopathogenic organisms belonging to the phyla Basidiomycota and Ascomycota. Nevertheless, the potential for resistance and the underlying resistance mechanisms of <em>Corynespora cassiicola</em> against florylpicoxamid are still not fully understood. We determined the baseline sensitivity levels of 101 <em>C. cassiicola</em> isolates to florylpicoxamid. The EC₅₀ values varied from 0.01 to 1.18 μg/mL with an average of 0.50 μg/mL. Laboratory-induced fungicide adaptation of nine wild-type isolates generated seven <em>C. cassiicola</em> mutants exhibiting high level of resistance to florylpicoxamid, all originating from a single parental isolate. The mutants maintained their resistance even after undergoing ten successive cultivations on a medium devoid of fungicides. No cross-resistance was detected between florylpicoxamid and pyraclostrobin, fluopyram, prochloraz, or propineb. Five of the resistant mutants showed an improved compound fitness index (CFI) relative to their parental isolate, whereas the remainder displayed either a reduced or comparable CFI. All seven of the resistant mutants displayed an A37V substitution within the CcCytb protein, which was responsible for the resistance to florylpicoxamid, as validated through molecular docking analysis. Furthermore, an allele-specific PCR (AS-PCR) method for detecting the CcCytb^A37V mutation was successfully established. In summarize, the findings of this study indicate a moderate risk of <em>C. cassiicola</em> developing resistance to florylpicoxamid, with the A37V substitution in CcCytb playing a key role in this resistance, detectable through the use of specific AS-PCR primers.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106228"},"PeriodicalIF":4.2,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic acid compounds isolated from Zanthoxylum armatum inhibit gray mold by disrupting cellular structures, affecting energy metabolism, and inducing oxidative stress of Botrytis cinerea","authors":"Yujie Liu ,&nbsp;Chengzhao Li ,&nbsp;Xingrong Wang ,&nbsp;Chu Gong ,&nbsp;Xianhua Meng ,&nbsp;Oybek Ergashev Karimovich ,&nbsp;Lei Sun ,&nbsp;Jun Wang ,&nbsp;Jun-Li Yang","doi":"10.1016/j.pestbp.2024.106229","DOIUrl":"10.1016/j.pestbp.2024.106229","url":null,"abstract":"<div><div><em>Botrytis cinerea</em> is a phytopathogenic fungus that utilizes multiple infection pathways and is known for causing gray mold on crops. This fungal infection results in the deterioration of crop quality and substantial economic losses. <strong>ZH-5</strong>, a phenolic acid compound derived from the methanol extract of the twigs of <em>Zanthoxylum armatum</em> DC., exhibited significant inhibitory effects on the mycelial growth, spore germination, and germ tube elongation of <em>B. cinerea</em> in <em>in vivo</em> experiments. The EC₅₀ of <strong>ZH-5</strong> was determined to be 58.96 mg L⁻¹, which surpassed the effectiveness of commonly used carbendazim and was comparable to the plant-derived pesticide eugenol. Our study focused on investigating the mechanisms underlying the antifungal activity of <strong>ZH-5</strong>. Our findings have demonstrated that <strong>ZH-5</strong> has the ability to disrupt the integrity of cellular structures, including mitochondria. This disruption affects energy metabolism and induces oxidative stress, ultimately <strong>ZH-5</strong> might be interfere with cellular activity or inhibit growth. In summary, we reported that <strong>ZH-5</strong> demonstrated efficacy as a plant-derived compound against <em>B. cinerea</em>, making it a potentially valuable environmentally-friendly chemical agent for the management of gray mold.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106229"},"PeriodicalIF":4.2,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparation of pheromone-binding proteins 1 and 2 of Spodoptera frugiperda in perceiving the three sex pheromone components Z9-14:Ac, Z7-12: Ac and Z11-16: Ac","authors":"Liming Hu ,&nbsp;Taoli Zhang ,&nbsp;Qingjun Wu ,&nbsp;Kangyuan Liang ,&nbsp;Guohui Yu ,&nbsp;Muyang He ,&nbsp;Dasong Chen ,&nbsp;Xiangning Su ,&nbsp;Yuping Zhang ,&nbsp;Zhenfei Zhang ,&nbsp;Jianmei Shen","doi":"10.1016/j.pestbp.2024.106183","DOIUrl":"10.1016/j.pestbp.2024.106183","url":null,"abstract":"<div><div>Pheromone-binding proteins (PBPs) are mainly responsible for binding and transporting hydrophobic pheromone molecules across the aqueous sensilla lymph to the receptor proteins. The preference of each PBP is believed to be different for each pheromone component within a single species. Significantly higher expression level of PBP1 and PBP2 in the male antennae of <em>Spodoptera frugiperda</em> suggesting that <em>Sfru</em>PBP1 and <em>Sfru</em>PBP2 might play important roles in pheromone perception. However, the preference of these two PBP to the three main pheromone components Z9-14: Ac, Z7-12: Ac and Z11-16: Ac have not been determined. In this study, a fluorescence competitive binding assay revealed that the binding intensities of <em>Sfru</em>PBP1 and <em>Sfru</em>PBP2 to Z9-14: Ac or Z7-12: Ac was comparable. We then used the CRISPR/Cas9 system to individually or simultaneously knock out PBP1 and PBP2 in <em>S.frugiperda</em>. The result of courtship behavior indicated that <em>Sfru</em>PBP1 and <em>Sfru</em>PBP2 were indispensable and played equal roles in perceiving the pheromones Z9-14: Ac and Z7-12: Ac for orientation, wing vibration, and hair-pencil display. Compared with Z9-14:Ac and Z7-12: Ac, Z11-16: Ac showed higher or medium binding intensities with <em>Sfru</em>PBP1 and <em>Sfru</em>PBP2 but played a minor role in inducing the wing vibration behavior. The results of this study are valuable for elucidating the mechanisms involved in sex pheromone perception and may facilitate the development of PBP-targeted pest control techniques.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"206 ","pages":"Article 106183"},"PeriodicalIF":4.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142748722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}