Pesticide Biochemistry and Physiology最新文献_第8页

Silica and mesoporous silica nanoparticles display effective insecticidal effect and augment plant defense responses 二氧化硅和介孔二氧化硅纳米颗粒显示出有效的杀虫效果，增强了植物的防御反应

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106389

Deepali Choudhary , Sneha Deshmukh , G. Maheswari , Archana Kumari , Vandana Ghormade

{"title":"Silica and mesoporous silica nanoparticles display effective insecticidal effect and augment plant defense responses","authors":"Deepali Choudhary , Sneha Deshmukh , G. Maheswari , Archana Kumari , Vandana Ghormade","doi":"10.1016/j.pestbp.2025.106389","DOIUrl":"10.1016/j.pestbp.2025.106389","url":null,"abstract":"<div><div>The polyphagous insect pest, <em>Spodoptera litura</em> displays insecticide resistance that requires new control tactics. In this context, inorganic silica nanoparticles (SiNPs) and mesoporous silica nanoparticles (MSNPs) were studied for their insecticidal activity and their effects on the plant defense responses. The synthesized silica (SiNPs,160 nm size) and mesoporous silica (MSNPs,100 nm size) nanoparticles showed high insecticidal effect of against <em>S. litura</em> larvae with 73.0 and 80.0 % mortalities, respectively at low nanoparticles concentration (1 μg). Administration of NPs by feeding enhanced the larval gut uptake and caused a significant ∼14.9- to 12.7-fold reduction in lactate dehydrogenase activity for SiNPs and MSNPs, respectively. Efficient uptake of fluorescent NPs was illustrated in columnar larval gut cells. Feeding of SiNPs and MSNPs led to a significant reduction in larval weight (2.9- and 3.4-fold, respectively) due to their antifeedant effect which was positively correlated to larval mortalities. Both NPs exhibited negligible cytotoxicity in vitro. Furthermore, application of rhodamine B fluorescence-tagged NPs on soyabean leaves showed NPs presence on the leaf surfaces and were not internalized by the leaf. Moreover, the electromechanical plant responses to NPs application displayed increased localized signal durations (>2-fold). Additionally, SiNPs and MSNPs treatments significantly upregulated the <em>12-oxophytodienoate reductase</em> plant jasmonic acid defense pathway gene expression (2.7- and 1.4-fold, respectively) that led to enhanced jasmonic acid contents. Application of SiNPs and MSNPs at low concentrations achieved insecticidal effect against <em>S. litura</em> and enhanced the plant defense responses against pest. Silica nanoparticles have potential in safe and effective management of <em>S. litura</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106389"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid spread of Amitraz resistance linked to a unique T115N mutation in the octopamine receptor of Varroa mites in Korea 在韩国，与瓦螨的章鱼胺受体中独特的T115N突变有关的阿米特拉兹耐药性的迅速蔓延

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106387

Joonhee Lee , Jong Hyeok Lee , Youngcheon Lim , Susie Cho , KyungHwan Moon , Sanghyeon Kim , Young Ho Kim , Si Hyeock Lee

{"title":"Rapid spread of Amitraz resistance linked to a unique T115N mutation in the octopamine receptor of Varroa mites in Korea","authors":"Joonhee Lee , Jong Hyeok Lee , Youngcheon Lim , Susie Cho , KyungHwan Moon , Sanghyeon Kim , Young Ho Kim , Si Hyeock Lee","doi":"10.1016/j.pestbp.2025.106387","DOIUrl":"10.1016/j.pestbp.2025.106387","url":null,"abstract":"<div><div>The <em>Varroa</em> mite, <em>Varroa destructor</em>, is an ectoparasitic pest of Western honey bees and poses a significant threat to apiculture. In Korea, widespread fluvalinate resistance has increased reliance on amitraz as an alternative acaricide. This study identified a novel mutation, T115N, in the β2-adrenergic-like octopamine receptor gene (<em>Octβ</em><sub><em>2</em></sub><em>R</em>) and confirmed its role in amitraz resistance. Genotyping of individual mites, based on time-dependent intoxication responses to a diagnostic dose of amitraz, revealed a strong correlation between genotype and resistance phenotype: mites with early intoxication responses were homozygous for the T115 allele, whereas those with delayed responses predominantly carried the homozygous N115 allele. Mites with homozygous N115 genotype exhibited a median lethal time approximately 2.8-fold longer than their T115 counterparts, confirming the resistance conferred by the T115N mutation. Quantitative sequencing was established to track the spread dynamics of T115N mutation in Korean <em>Varroa</em> mite populations over five years (2020–2024). While the mutation was rare from 2020 to 2022, its frequency increased sharply in 2023 and became widespread across Korea by 2024, coinciding with increased amitraz usage. These findings suggest region-specific selection for the T115N mutation driven by amitraz exposure.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106387"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The involvement of thioredoxin reductase genes in development, reproduction and deltamethrin tolerance in the red flour beetle, Tribolium castaneum 硫氧还蛋白还原酶基因在红粉甲虫发育、繁殖和溴氰菊酯耐受性中的作用

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106390

Caihong Ji , Daojie Guan , Haoting Chen , Zhichao Luo , Chengyun Jian , Zhichao Wang , Huichen Ge , Kun Qian , Jianjun Wang

{"title":"The involvement of thioredoxin reductase genes in development, reproduction and deltamethrin tolerance in the red flour beetle, Tribolium castaneum","authors":"Caihong Ji , Daojie Guan , Haoting Chen , Zhichao Luo , Chengyun Jian , Zhichao Wang , Huichen Ge , Kun Qian , Jianjun Wang","doi":"10.1016/j.pestbp.2025.106390","DOIUrl":"10.1016/j.pestbp.2025.106390","url":null,"abstract":"<div><div>As an essential component of the thioredoxin system, thioredoxin reductase (TrxR) plays an important role in maintaining redox homeostasis in mammalian cells, however, functional characterization of insect TrxRs is still limited. In this study, full-length cDNAs of <em>TcTrxR1</em> and <em>TcTrxR2</em> were cloned from the red flour beetle, <em>Tribolium castaneum.</em> Sequence analysis revealed the highly conserved active site motifs CVNVGC and CCS at the N-terminal and C-terminal of TcTrxR1, respectively, whereas TcTrxR2 lacks these two conserved motifs. Analysis of the spatio-temporal expression pattern by RT-qPCR showed that the expression of <em>TcTrxR1</em> was the highest in 1-day-old larva and brain, and <em>TcTrxR2</em> was highly expressed in eggs and fat body, respectively. Further functional analysis by RNA interference (RNAi) revealed that knockdown of <em>TcTrxR1</em> and <em>TcTrxR2</em> at the larval stage led to 100 % and 98.67 % mortality of larvae beetles, and pupal RNAi of <em>TcTrxR1</em> and <em>TcTrxR2</em> resulted in decreased eclosion rates as well as failure of the female adults to lay eggs. Additionally, injection of dsTcTrxR2 decreased the tolerance of beetles to deltamethrin, whereas knockdown of <em>TcTrxR1</em> significantly increased the tolerance of beetles to deltamethrin. Notably, knockdown of <em>TcTrxR1</em> significantly upregulated the expression of <em>TcCYP6BQ2</em>, <em>TcCYP6BQ4</em> and <em>TcCYP6BQ7</em>, and led to nuclear translocation of transcription factor CncC, a major regulator of detoxification in insects. These findings provide insights into the function of insect <em>TrxRs</em> as well as the regulatory mechanisms of CncC, and have applied implications for the RNAi-based insect pest control.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106390"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional analysis of Ophraella communa Lesage OcomOBP11 in recognition of Ambrosia artemisiifolia L. volatiles 鸢尾草OcomOBP11识别艾草挥发物的功能分析

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106392

Yang Yue , Chao Ma , Yan Zhang , Wei-hua Ma , Jing-jing Wang , Zhen-ya Tian , Guang-mei Chen , Ru-wen Li , Jin-hua Li , Jing-fang Yang , Zhong-shi Zhou

{"title":"Functional analysis of Ophraella communa Lesage OcomOBP11 in recognition of Ambrosia artemisiifolia L. volatiles","authors":"Yang Yue , Chao Ma , Yan Zhang , Wei-hua Ma , Jing-jing Wang , Zhen-ya Tian , Guang-mei Chen , Ru-wen Li , Jin-hua Li , Jing-fang Yang , Zhong-shi Zhou","doi":"10.1016/j.pestbp.2025.106392","DOIUrl":"10.1016/j.pestbp.2025.106392","url":null,"abstract":"<div><div>Odorant binding proteins (OBPs) play a critical role in insect survival by facilitating the detection of environmental odors. This study identified and characterized <em>OcomOBP11</em> in the leaf beetle <em>Ophraella communa</em> Lesage. Sequence and phylogenetic analyses demonstrated that <em>OcomOBP11</em> belongs to the Minus-C OBP family. Tissue-specific expression analysis revealed that <em>OcomOBP11</em> showed the highest relative expression in the testis. Functional assays demonstrated that OcomOBP11 has strong binding affinity to 24 of the 26 volatiles from <em>Ambrosia artemisiifolia</em> L., a primary host plant. Molecular docking showed that OcomOBP11 formed hydrophobic interactions with β-caryophyllene, α-pinene, camphene, octanal, and nonanal and hydrogen bonds with octanal and nonanal. RNAi-mediated knockdown of OcomOBP11 significantly reduced the electrophysiological (EAG) response and behavioral preference of <em>O. communa</em> for these ligands. These findings suggest that OcomOBP11 plays an important role in host plant localization, thus providing guidance for future biological invasion control strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106392"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bombyx mori nuclear polyhedrosis virus infection regulated by host glycosphingolipids 寄主鞘糖脂调控家蚕核型多角体病毒感染

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106388

Feifei Zhu , Jindie Hong , Tingting Xue , Qi Tang , Qian Yu , Guohui Li , Shangshang Ma , Xiaoyong Liu , Shuhao Huo , Keping Chen

{"title":"Bombyx mori nuclear polyhedrosis virus infection regulated by host glycosphingolipids","authors":"Feifei Zhu , Jindie Hong , Tingting Xue , Qi Tang , Qian Yu , Guohui Li , Shangshang Ma , Xiaoyong Liu , Shuhao Huo , Keping Chen","doi":"10.1016/j.pestbp.2025.106388","DOIUrl":"10.1016/j.pestbp.2025.106388","url":null,"abstract":"<div><div>Glycosylation is an important post-translational modification commonly found in eukaryotes, and plays crucial roles in many biological activities. The silkworm <em>Bombyx mori</em> (<em>B. mori</em>), an important economic insect and a model organism in biology, has recently been found to be abundantly glycosylated. In this study, we established the role of silkworm glycosphingolipids (GSLs), the glycoconjugates formed by covalent attachment of a glycan to the lipid class of ceramide, during <em>B. mori</em> nuclear polyhedrosis virus (BmNPV) infection. The levels of cellular glycosphingolipids (GSLs), particularly the glucosylceramide (Glc-Cer) series, were modulated by targeting uridine diphosphate-glucose ceramide glycosyltransferase (UGCG), the enzyme responsible for Glc-Cer synthesis. Inhibiting UGCG activity by Genz-123346 (Genz), an inhibitor and substrate analogue of UGCG, reduced BmNPV binding, internalization, and viral protein expression in BmN cells. A general reduction in the cellular GSL contents was observed following Genz treatment. Overexpression of UGCG increased cellular GSL levels overall while still caused suppression in viral infection. It is postulated that GSLs are highly regulated membrane components that are crucial for viral entry, and disturbing the balance, either by increasing or decreasing cellular GSL components, alters membrane traffic and transport, which is unfavorable for viral infection. Therefore, highly regulated cellular GSLs are required for effective BmNPV infection. This study provides direct evidence linking GSL levels to BmNPV infection, offering new insights into the role of GSLs in viral infection.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106388"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of short-term ivermectin exposures on fruit flies 短期暴露于伊维菌素对果蝇的影响

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-24 DOI: 10.1016/j.pestbp.2025.106391

M. Yusuf Ali , Carl K. Namini , John M. Clark , Barry R. Pittendrigh , Si H. Lee , Kyong S. Yoon

{"title":"Impacts of short-term ivermectin exposures on fruit flies","authors":"M. Yusuf Ali , Carl K. Namini , John M. Clark , Barry R. Pittendrigh , Si H. Lee , Kyong S. Yoon","doi":"10.1016/j.pestbp.2025.106391","DOIUrl":"10.1016/j.pestbp.2025.106391","url":null,"abstract":"<div><div>A short-term ivermectin (IVM) exposure method was newly established to demonstrate effects of sublethal concentrations of IVM on the wild-type fruit fly, <em>Drosophila melanogaster</em>. Using a conventional glass-vial contact approach, exposures to IVM (0.01 to 1000 ppm) for 12 h durations or less were selected to assess the downstream impacts of short-term IVM exposures (STIEs) on fruit flies. Under these conditions, all female flies produced significantly higher levels of reactive oxygen species and malondialdehydes in their ovaries. Additionally, females treated with IVM for 12 h under the STIE conditions exhibited significantly increased levels of DNA damages in their ovaries. Despite the negative impacts described above, the mean percent hatchability values obtained from the eggs oviposited by the IVM-exposed females were not statistically different when compared to the hatchability of the unexposed females. Two concentrations (1 and 10 ppm) of IVM were selected to determine transgenerational effects following short-term IVM exposures. F1, F2 and F8 flies exposed to IVM showed significantly delayed developments (2.5–3.2, 2.5–3.0, and 0.9–1.3 days delayed, respectively). F5, F11 and F17 females showed significantly delayed IVM-induced sluggish behaviors in the presence of lethal IVM (1 %, <em>w</em>/<em>v</em>). F18 females transgenerationally exposed to 1 ppm IVM exhibited significantly increased levels of <em>Mrp1</em> (8.7-fold) and <em>Cyp6g2</em> (5.9-fold) transcripts compared to unexposed flies. Comparatively, F18 females transgenerationally exposed to 10 ppm IVM showed significantly increased levels of <em>Cyp9f2</em> (2.6-fold) transcripts. Current study clearly demonstrated the effects of sublethal IVM on parent and filial generations of fruit flies, providing an important step toward understanding development of IVM resistance under the STIE conditions.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106391"},"PeriodicalIF":4.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Trp-574-Leu mutations together with enhanced metabolism contribute to cross-resistance to ALS inhibiting herbicides in Fimbristylis littoralis Trp-574-Leu突变与代谢增强共同促进了滨金缕草对ALS抑制除草剂的交叉抗性

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-23 DOI: 10.1016/j.pestbp.2025.106385

Yueyue Shi , Zhiwen Wu , Rui Cheng , Lilei Zhang , Xiaoxiao Guo , Xuefeng Li , Yaling Bi

{"title":"The Trp-574-Leu mutations together with enhanced metabolism contribute to cross-resistance to ALS inhibiting herbicides in Fimbristylis littoralis","authors":"Yueyue Shi , Zhiwen Wu , Rui Cheng , Lilei Zhang , Xiaoxiao Guo , Xuefeng Li , Yaling Bi","doi":"10.1016/j.pestbp.2025.106385","DOIUrl":"10.1016/j.pestbp.2025.106385","url":null,"abstract":"<div><div>Abstract</div><div><em>Fimbristylis littoralis</em> Gaudich., an important weed in Chinese paddy fields, has caused significant yield losses in rice and other crops. Acetolactate synthase (ALS) inhibitors, such as halosulfuron-methyl, are widely used for weed control. This study identified a highly resistant population (R23–1) of <em>F. littoralis</em> to halosulfuron-methyl, with an exceptionally high resistance index (RI) of 3441.66. The resistant mechanisms of <em>F. littoralis</em> to ALS inhibitors have not been reported previously. We employed a comprehensive approach to address this, including whole-plant bioassay, ALS target gene sequencing, molecular docking, synergistic tests with metabolic enzyme inhibitors, glutathione S-transferases (GSTs) activity assays, and cross-resistance testing. The results revealed the first report of a Trp-574-Leu mutation in the <em>ALS</em> gene of the R23–1 population, which significantly increased binding energy, as shown by molecular docking analysis. Synergistic tests demonstrated that the cytochrome P450 monooxygenase (P450) inhibitor piperonyl butoxide (PBO) and the GSTs inhibitor 4-chloro-7-nitro-1,2,3-benzoxadiazole (NBD-Cl) markedly enhanced the sensitivity of the R23–1 population to halosulfuron-methyl, with synergistic ratios of 4.11 and 8.15, respectively, while malathion had no effect. GST activity decreased in both populations after halosulfuron-methyl treatment, with the R23–1 population consistently showing significantly higher levels, peaking on day five. Furthermore, the R23–1 population demonstrated cross-resistance to multiple ALS inhibitors. These findings provide novel insights into the resistance mechanisms of <em>F. littoralis</em> and lay a theoretical foundation for developing effective strategies to mitigate or delay the evolution of resistance.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106385"},"PeriodicalIF":4.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reverse engineering high-level resistance to Bt Cry1Ac toxin in Plutella xylostella reveals a hormonal regulatory feedback pathway 对小菜蛾高水平抗Cry1Ac毒素的逆向工程揭示了激素调控反馈途径

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-22 DOI: 10.1016/j.pestbp.2025.106382

Dan Sun , Mingyun Wang , Le Guo , Xuping Shentu , Xiaoping Yu , Neil Crickmore , Xuguo Zhou , Youjun Zhang , Zhaojiang Guo

{"title":"Reverse engineering high-level resistance to Bt Cry1Ac toxin in Plutella xylostella reveals a hormonal regulatory feedback pathway","authors":"Dan Sun , Mingyun Wang , Le Guo , Xuping Shentu , Xiaoping Yu , Neil Crickmore , Xuguo Zhou , Youjun Zhang , Zhaojiang Guo","doi":"10.1016/j.pestbp.2025.106382","DOIUrl":"10.1016/j.pestbp.2025.106382","url":null,"abstract":"<div><div>Decoding the molecular mechanisms of insect resistance to <em>Bacillus thuringiensis</em> (Bt) toxins is crucial for the sustainable utilization of Bt-based bioinsecticides and transgenic crops. Our previous studies showed that a hormone-responsive transcription factor FOXO binds to an inserted short interspersed nuclear element (SINE, named SE2), causing <em>MAP4K4</em> overexpression and resistance to Bt Cry1Ac toxin in <em>Plutella xylostella</em>. Furthermore, titers of two upstream signaling hormones (20-hydroxyecdysone and juvenile hormone) were also found to be elevated in the resistant strain, but it was unclear whether this was due to natural variation or a feedback pathway. Here, we established a homozygous knock-in strain (SE2-KI) using a reverse genetic approach to insert the SE2 retrotransposon into the <em>MAP4K4</em> promoter of a Cry1Ac-susceptible strain. The SE2 insertion induced <em>MAP4K4</em> overexpression, which in turn caused a downregulation of midgut receptors and an identical resistance phenotype to that seen in the evolved resistant strain. Moreover, SE2 insertion significantly increased the levels of two insect hormones providing definitive evidence for a positive feedback regulatory pathway. This study unveils an as yet uncharacterized hormonal regulatory feedback pathway orchestrating Cry1Ac resistance in <em>P. xylostella</em>, providing new insights into the molecular basis of Bt resistance and informing suitable field resistance management strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106382"},"PeriodicalIF":4.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytochrome CYP72A15 may play a role in metabolic resistance to mesotrione in wild radish 细胞色素CYP72A15可能在野生萝卜对美索三酮的代谢抗性中起作用

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-19 DOI: 10.1016/j.pestbp.2025.106380

Feng-Yan Zhou , Huan Lu , Xueping Huang , Yunjing Han , Yong Zhang , Heping Han , Liudmyla Tsykalchuk , Alex Nyporko , Qin Yu

{"title":"Cytochrome CYP72A15 may play a role in metabolic resistance to mesotrione in wild radish","authors":"Feng-Yan Zhou , Huan Lu , Xueping Huang , Yunjing Han , Yong Zhang , Heping Han , Liudmyla Tsykalchuk , Alex Nyporko , Qin Yu","doi":"10.1016/j.pestbp.2025.106380","DOIUrl":"10.1016/j.pestbp.2025.106380","url":null,"abstract":"<div><div>In our previous study with a wild radish (<em>Raphanus raphanistrum)</em> population resistant to HPPD-inhibiting herbicides we indicated that two additional candidate P450 genes, <em>CYP71A28</em> and <em>CYP72A13</em>-like, may also contribute to the resistance. This study investigates the role of these two P450 genes in mesotrione resistance in the wild radish population. The full-length <em>R. raphanistrum</em> P450 genes (thereafter named as <em>RrCYP71A28</em> and <em>RrCYP72A15)</em> were cloned from mesotrione-resistant (R) and -susceptible (S) wild radish plants. RT-qPCR results showed that basal expression levels of the two P450 genes are significantly higher (up to 3-fold) in the R than the S plants. <em>Escherichia coli</em> cells transformed respectively with <em>RrCYP71A28</em> and <em>RrCYP72A15</em> were more tolerant to mesotrione. Transgenic Arabidopsis plants expressing <em>RrCYP72A15</em> showed a modest level of resistance to mesotrione. UPLC-MS/MS analysis demonstrated that tissue mesotrione levels in <em>RrCYP72A15</em> transgenic Arabidopsis plants were significantly lower (up to 2-fold) than that in the wild type. Structural modelling predicts CYP72A15 can bind to RrCYP72A15 and metabolize mesotrione likely through formation of 4-OH-mesotrione. Although the <em>RrCYP72A15</em> gene confers a modest level of resistance, overexpression of the multiple herbicide-metabolizing genes could contribute to the low level of mesotrione resistance observed in the R wild radish population.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"210 ","pages":"Article 106380"},"PeriodicalIF":4.2,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the mode of action of BtEcR/USP-LBD with benzpyrimoxan in combination with high throughput SPR screening and molecular simulation approaches 结合高通量SPR筛选和分子模拟方法，了解BtEcR/USP-LBD与benzpyrimoxan的作用模式

IF 4.2 1区农林科学

Pesticide Biochemistry and Physiology Pub Date : 2025-03-18 DOI: 10.1016/j.pestbp.2025.106384

Hongyan Wang , Jialin Cui , Yanjiao Feng , Xinpeng Sun , Qinyan Tan , Li Zhang

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