Pesticide Biochemistry and Physiology最新文献

{"title":"Corrigendum to “Biological and molecular characterizations of field fludioxonil-resistant isolates of Fusarium graminearum” [Pesticide Biochemistry and Physiology 184 (2022) 105101]","authors":"Ziyue Wen, Jingrui Wang, Chen Jiao, Wenyong Shao, Zhonghua Ma","doi":"10.1016/j.pestbp.2024.106144","DOIUrl":"10.1016/j.pestbp.2024.106144","url":null,"abstract":"","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106144"},"PeriodicalIF":4.2,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0048357524003778/pdfft?md5=e3c7eb44f6010fa32391a25239d17250&pid=1-s2.0-S0048357524003778-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baseline susceptibility, risk assessment of resistance, changes in insecticides sensitivity and fitness after selection of dimpropyridaz in Aphis gossypii Glover (Hemiptera: Aphididae)","authors":"Jiao Shang ,&nbsp;Haishan Wang,&nbsp;Wenyang Dong,&nbsp;Xinyu Guo,&nbsp;Jiahao Zhu,&nbsp;Pei Liang,&nbsp;Xueyan Shi","doi":"10.1016/j.pestbp.2024.106138","DOIUrl":"10.1016/j.pestbp.2024.106138","url":null,"abstract":"<div><div>Dimpropyridaz as a novel insecticide is registered for the management of many aphid species and other piercing-sucking insects. For the aim to clarify the susceptibility of natural population and the resistance risk of dimpropyridaz in <em>Aphis gossypii</em> in China, the baseline susceptibility, risk assessment, changes of insecticides sensitivity and fitness after selection of dimpropyridaz in <em>A. gossypii</em> were evaluated in present study. The results showed that dimpropyridaz exhibited high activity against 23 field populations of <em>A. gossypii</em> from cotton planting regions in China with LC₅₀ values ranging from 0.37 to 8.93 mg/L, which indicating a 24.14-fold natural susceptibility variability between the most sensitive and the most selected populations. Based on the dimpropyridaz susceptibilities of 23 field populations, the sensitivity baseline of <em>A. gossypii</em> to dimpropyridaz was established with the LC₅₀ value as 1.29 mg/L. Additionally, the resistance risk of <em>A. gossypii</em> to dimpropyridaz was evaluated by continuously selecting both a susceptible laboratory population and a field Tulufan population. After 10 selections with dimpropyridaz, no significant dimpropyridaz resistance increased in both populations, with the realized heritability were estimated as 0.01 and 0.04 for the two populations respectively, and this meant low resistance risk to dimpropyridaz of <em>A. gossypii.</em> In addition, the sensitivities of the dimpropyridaz selected population to imidacloprid, acetamiprid and sulfoxaflor displayed some reduction, whereas no significant changes in sensitivity to afidopyropen, carbosulfan and bifenthrin. However, the dimpropyridaz selected population showed a low fitness, with fitness as 0.73. These results provide important information for development of effective resistance management strategies of dimpropyridaz in <em>A. gossypii</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106138"},"PeriodicalIF":4.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNAi-mediated silencing of NlGRP3 augments the insecticidal virulence of Metarhizium anisopliae to the brown planthopper Nilaparvata lugens","authors":"Zhu-Long Shao,&nbsp;Chen-Ping Lan,&nbsp;Xiao-Ping Yu,&nbsp;Zheng-Liang Wang","doi":"10.1016/j.pestbp.2024.106141","DOIUrl":"10.1016/j.pestbp.2024.106141","url":null,"abstract":"<div><p>The rapid development of insecticide resistance reinforces the urgent need to develop eco-friendly strategies for controlling <em>Nilaparvata lugens</em> (brown planthopper, BPH), the most destructive insect pest of rice. Both entomopathogens and RNA interference (RNAi) provide attractive alternatives to chemical insecticides. In this study, we demonstrated the synergistic potential of the combination use of entomopathogen- and RNAi-mediated approaches to control BPH. The β-1, 3-glucan recognition protein (βGRP) encoding gene <em>NlGRP3</em> was identified and its potential role in immune defense was characterized in BPH. The open reading frame (ORF) of <em>NlGRP3</em> is 1740 bp in length, encoding a 65.8 kDa protein with conserved CBM39 and GH16 domains that typically existed in the βGRP family members. <em>NlGRP3</em> was shown to be differentially expressed across developmental stages and highly transcribed in the immune responsive tissues haemolymph and fat body. Topical infection with a fungal entomopathogen <em>Metarhizium anisopliae</em> could significantly up-regulate its expression level. RNAi-mediated silencing of <em>NlGRP3</em> resulted in significantly decreased survival rate and increased susceptibility to fungal challenge in the fifth-instar BPH nymphs. The greatly enhanced mortality of <em>NlGRP3</em>-silenced BPH following fungal infection might be in part directly due to the immune suppression by down-regulating expressions of antimicrobial peptide genes and the imbalance of the bacterial community harboring in BPH body. Our results highly demonstrated that suppressing the insect innate immune defense through RNAi targeting the immune-related genes could effectively strengthen the biocontrol efficacy of fungal entomopathogens, providing clues to the combination use of RNAi and entomopathogens as a promising approach for BPH control.</p></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106141"},"PeriodicalIF":4.2,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the role of the ROS/CncC-xenobiotics signaling pathway in the response to Fenpropathrin in Cyprinus carpio lymphocytes: Involving lipid peroxidation and Fe2+ metabolism imbalance","authors":"Lei Diao ,&nbsp;Jing Gao ,&nbsp;Yuxun Zhou ,&nbsp;Liping Wang ,&nbsp;Xiaowei Yang ,&nbsp;Peng Li ,&nbsp;Jingying Zhai ,&nbsp;Yang Ma ,&nbsp;Huijie Chen","doi":"10.1016/j.pestbp.2024.106142","DOIUrl":"10.1016/j.pestbp.2024.106142","url":null,"abstract":"<div><p>Fenpropathrin (FPT) is a synthetic pyrethroid insecticide, the persistence and accumulation in water of which could cause harmful effects on vulnerable groups like aquatic creatures, particularly posing significant risks to fish immune systems. This study aimed to investigate how environmentally relevant FPT concentrations (10–1000 μ/M) affect lipid peroxidation and Fe²⁺ metabolism in <em>Cyprinus carpio</em> head kidney lymphocytes, and its relationship with oxidative stress and immunotoxicity. Firstly, CCK-8 results demonstrated that FPT caused a significant increase in lymphocyte death. Secondly, lymphocytes exposed to FPT could lead ferroptosis in lymphocytes, accompanied by evidence of the Fe²⁺ transporter imbalance, lipid peroxidation, Fe²⁺ accumulation and ferroptosis related protein increment. Thirdly, we found that FPT esposure leads to a decrease in ATP, mitochondrial DNA and NADPH/NADP⁺ levels, and the mRNA associated with mitochondrial function-related genes (Fis1, Drp1, and OPA1) in lymphocytes. Additionally, FPT induced the increased the levels of inflammatory genes (TNF-α, IFN-γ, and IL-6) in head kidney lymphocytes. Importantly, exposure to FPT induced oxidative stress to produce intracellular ROS, disrupting the function of the CncC signaling pathway and expression disorder of xenobiotics detoxification (CYP 450 family) genes. Notably, Treatment with NAC (a ROS inhibitor, 5 μM) demonstrated that inhibiting ROS alleviated FPT-induced lymphocyte ferroptosis and inflammatory response via the ROS/CncC-xenobiotics signaling pathway. These findings not only introduces a novel approach to investigating the immunotoxicity of FPT but also offers critical insights into mitigating the adverse effects of FPT on aquatic animal health.</p></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106142"},"PeriodicalIF":4.2,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of TcGluCl leads to the premature pupation of Tribolium castaneum larvae possibly by influencing the calcium-mediating hormone homeostasis","authors":"Xi Zeng ,&nbsp;Chengyun Jiang ,&nbsp;Xu Zhao ,&nbsp;Zhaolu Wu ,&nbsp;Anxiang Zhuang ,&nbsp;Kun Qian ,&nbsp;Jianjun Wang ,&nbsp;Xiangkun Meng","doi":"10.1016/j.pestbp.2024.106137","DOIUrl":"10.1016/j.pestbp.2024.106137","url":null,"abstract":"<div><p>The glutamate-gated chloride channels (GluCls) are widely existed in the neural and nonneural tissues of invertebrate. In addition to play important roles in signal transduction, the GluCls also showed multiple physiological functions in insects such as participate in the juvenile hormone synthesis. In the present study, the potential roles of <em>TcGluCl</em> in growth and development of the red flour beetle <em>Tribolium castaneum</em> were explored. Knockdown of <em>TcGluCl</em> showed no effects on the survivability, weight growth, final pupation rate, eclosion and fecundity of <em>T. castaneum</em>, whereas resulted in the significant premature pupation of larvae. Inhibition of <em>TcGluCl</em> expression significantly changed the levels of juvenile hormone and ecdysone as well as the expressions of hormone biosynthetic genes. The increased ecdysone level and decreased juvenile hormone level were observed at the late stage of dsGluCl-treated larvae. Knockdown of <em>TcGluCl</em> significantly reduced the expressions of <em>TcSTIM1</em> and <em>TcOrai1</em>, which were the primary proteins in store-operated calcium entry (SOCE) mediated Ca²⁺ influx mechanism. Whilst the L-glutamic acid treatment led to the increased <em>TcOrai1</em> expression in <em>T. castaneum</em>. These findings suggested that knockdown of <em>TcGluCl</em> increased the ecdysone level and contributed to the premature pupation of larvae, which might be due to the reduced Ca²⁺ influx caused by the decreased expressions of <em>TcSTIM1</em> and <em>TcOrai1</em>. These studies provide novel insights on the function of GluCls in insects.</p></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106137"},"PeriodicalIF":4.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance monitoring of diamide insecticides and characterization of field-evolved chlorantraniliprole resistance among Chinese populations of the tomato pinworm Phthorimaea (=Tuta) absoluta (Lepidoptera: Gelechiidae)","authors":"Xiaoli Ma ,&nbsp;Cheng Qu ,&nbsp;Jiaqi Yao ,&nbsp;Jixing Xia ,&nbsp;Chen Luo ,&nbsp;Raul Narciso C. Guedes ,&nbsp;Ran Wang","doi":"10.1016/j.pestbp.2024.106140","DOIUrl":"10.1016/j.pestbp.2024.106140","url":null,"abstract":"<div><p>The tomato pinworm, <em>Phthorimaea</em> (=<em>Tuta</em>) <em>absoluta,</em> is considered one of the most destructive and invasive insect pests worldwide, having developed significant resistance to many popular insecticides. In this study, we monitored the field resistance of <em>P. absoluta</em> populations from China to three diamide insecticides: flubendiamide, chlorantraniliprole, and cyantraniliprole. We found that one field population from Wuzhong City (WZ) exhibited high level of resistance to chlorantraniliprole. Using the WZ population and a susceptible reference strain (YN-S), we established a near-isogenic line (WZ-NIL) of <em>P. absoluta</em> with resistance to chlorantraniliprole. This strain also showed substantial cross-resistance to flubendiamide, and cyantraniliprole. Genetic analysis revealed that the inheritance of resistance to chlorantraniliprole in the WZ-NIL strain was autosomal and incompletely dominant. Additionally, the pesticide synergist piperonyl butoxide significantly inhibited chlorantraniliprole resistance by compromising P450 monooxygenase activity, which was significantly higher in the resistant strain. Furthermore, WZ-NIL had significantly prolonged developmental stages, lower pupation rates, reduced female fecundity, and lower egg hatchability than YN-S individuals. The fitness of WZ-NIL relative to YN-S was estimated to be 0.73, indicating significant fitness cost associated with chlorantraniliprole resistance. Rotating chlorantraniliprole with other insecticides that have different modes of action and degradation may be particularly useful for managing chlorantraniliprole resistance in <em>P. absoluta</em>.</p></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106140"},"PeriodicalIF":4.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of neuropeptide prothoracicotropic hormone (PTTH) - Torso in pyriproxyfen-induced larval-pupal abnormal metamorphosis in silkworms","authors":"Chunhui He ,&nbsp;Yizhe Li ,&nbsp;Zhenfeng Zhou ,&nbsp;Yuting Wei ,&nbsp;Yizhou Zhu ,&nbsp;Yirong Han ,&nbsp;Yifei Li ,&nbsp;Rifeng Yang ,&nbsp;Kaizun Xu","doi":"10.1016/j.pestbp.2024.106139","DOIUrl":"10.1016/j.pestbp.2024.106139","url":null,"abstract":"<div><p>The neuropeptide prothoracicotropic hormone (PTTH) plays a key role in regulating ecdysone synthesis and promoting insect metamorphosis. Pyriproxyfen is a juvenile hormone analogue. We previously reported that pyriproxyfen disrupts ecdysone secretion and inhibits larval-pupal metamorphosis in silkworms. However, the specific molecular mechanisms by which pyriproxyfen interferes with ecdysone signaling remain to be elucidated. Herein, the RNA-seq analysis on the ecdysone-secretion organ prothoracic gland (PG) was conducted following pyriproxyfen exposure. A total of 3774 differentially expressed genes (DEGs) were identified, with 1667 up-regulated and 2107 down-regulated. KEGG analysis showed that DEGs were enriched in the MAPK signaling pathway, a conserved pathway activated by PTTH binding to Torso, which regulates the ecdysone synthesis. qRT-PCR results indicated a significant up-regulation in <em>PTTH</em> transcription level, while the transcription levels of <em>torso</em> and downstream MAPK pathway genes, <em>Ras2</em>, <em>Raf</em> and <em>ERK</em>, were down-regulated 24 h post-pyriproxyfen treatment. Consistent with these transcriptional changes, PTTH titers in the brain also increased following pyriproxyfen treatment. These results suggest that pyriproxyfen induces abnormal metamorphosis in silkworms by impairing PTTH-Torso signaling. This study enhances our understanding of the molecular mechanisms of pyriproxyfen-induced larval-pupal abnormal metamorphosis in silkworms, and also provides insights for developing detoxification strategies for juvenile hormone analog pesticides to non-target organisms.</p></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106139"},"PeriodicalIF":4.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of camptothecin exposures on the development and larval midgut metabolomic profiles of Spodoptera frugiperda","authors":"Xuanyue Yu ,&nbsp;Xinyi Xie ,&nbsp;Cuiting Liu ,&nbsp;Yuting Huang ,&nbsp;Huiyin Hu ,&nbsp;Jian Zeng ,&nbsp;Benshui Shu ,&nbsp;Jingjing Zhang","doi":"10.1016/j.pestbp.2024.106136","DOIUrl":"10.1016/j.pestbp.2024.106136","url":null,"abstract":"<div><p><em>Spodoptera frugiperda</em> is an economic agricultural pest that has invaded many countries around the world and caused huge losses in grain production. Camptothecin (CPT) is one of the botanical compounds with insecticidal activity and has the potential for pest control. However, the effects of CPT on development and metabolism of <em>S. frugiperda</em> remain unknown. In this study, we have investigated the adverse effects of 1.0 and 5.0 mg/kg CPT exposures on the growth and development of <em>S. frugiperda</em>. Our results found that 1.0 and 5.0 mg/kg CPT treatments altered the parameters of the life cycle, including inducing larval mortality, altering the weight of larvae, pupae, and adults, the larval duration, and decreasing the pupation rate and emergence rate. In addition, comparative metabolomics analysis was performed in the larval midgut of <em>S. frugiperda to</em> explore the toxicity mechanism of CPT. A total of 261 and 348 differential metabolites were identified in the groups with 1.0 and 5.0 mg/kg CPT treatments, respectively. Further analysis found that pantothenate and CoA biosynthesis, sulfur relay system, selenocompound metabolism, and fatty acid biosynthesis pathways were significantly altered by 5.0 mg/kg CPT exposure. Our results provided new insight into the toxicological mechanisms of CPT against <em>S. frugiperda</em> and laid the foundation for the field application of CPT in pest control.</p></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106136"},"PeriodicalIF":4.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The two-component histidine kinase BdHk1 regulates fungal development, virulence and fungicide sensitivity in Botryosphaeria dothidea","authors":"Meiqi Zhu ,&nbsp;Tinghua Huan ,&nbsp;Yanru Ma,&nbsp;Yang Han,&nbsp;Na Liu,&nbsp;Sen Lian,&nbsp;Baohua Li,&nbsp;Weichao Ren","doi":"10.1016/j.pestbp.2024.106134","DOIUrl":"10.1016/j.pestbp.2024.106134","url":null,"abstract":"<div><div>Histidine kinases (HKs) allow fungal cells to sense and respond to environmental stimuli. However, the biological role of HKs in <em>Botryosphaeria dothidea</em>, the causal agent of Botryosphaeria canker and apple ring rot, remains unknown. In this study, we identified and characterized the two-component histidine kinase BdHk1 in <em>B. dothidea</em>. Targeted knockout of <em>BdHK1</em> gene resulted in severe conidiation and pathogenicity defects. In addition, the Δ<em>BdHk1</em> mutant showed hypersensitivity to osmotic stress, but resistance to phenylpyrrole and dicarboximide fungicides. Moreover, the Δ<em>BdHk1</em> mutant exhibited significantly increased sensitivity to the cell membrane-damaging agent SDS and high temperature. Comparative transcriptome analysis revealed that inactivation of BdHk1 influenced multiple metabolic pathways in <em>B. dothidea</em>. Taken together, our results suggest that BdHk1 plays an important role in development, virulence and stress tolerance in <em>B. dothidea</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106134"},"PeriodicalIF":4.2,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salicylic acid functionalized chitosan nanocomposite increases bioactive components and insect resistance of Agastache rugosa","authors":"Huimin Liao ,&nbsp;Jinghong Wang ,&nbsp;Feiran Chen ,&nbsp;Le Ya ,&nbsp;Ningke Fan ,&nbsp;Yinghua Shu ,&nbsp;Zhenggao Xiao ,&nbsp;Zhenyu Wang","doi":"10.1016/j.pestbp.2024.106131","DOIUrl":"10.1016/j.pestbp.2024.106131","url":null,"abstract":"<div><p>The abundance of monoterpenoids and phenolic compounds determines the medicinal quality and anti-insect properties of <em>Agastache rugosa</em>, which can be compromised by biotic stress such as herbivore attacks. The traditional use of chemical pesticides to mitigate herbivore interference is increasingly incompatible with sustainable agriculture. In response, nanotechnology-based biostimulants, which can activate metabolic processes to enhance plant growth and stress resistance, offer a more cost-effective and environmentally-friendly alternative. However, to date, it remains unknown how nano-biostimulants improve the therapeutic value and insect resistance of medicinal plants simultaneously. This study investigates the effect of 0–1000 mg/L of a nano-biostimulant salicylic acid functionalized chitosan nanocomposite (SCN) on the pharmacological and anti-herbivore properties of medicinal plant <em>A. rugosa</em>. Results showed that 100 mg/L SCN significantly inhibited <em>Spodoptera litura</em> growth by 62.9 %, and increased plant shoot and root biomass by 107.2 % and 77.6 %, respectively. Moreover, 100 mg/L SCN significantly upregulated the expression of the key genes (<em>e.g.</em>, <em>LS</em>, <em>L3OH</em>, and <em>CHS</em>) involved in monoterpene and phenolic compounds biosynthesis by 1.4–10.1 folds, thus boosting the production of active compounds such as pulegone, β-myrcene, and chlorogenic acid by 1.5–24.4 folds. These enhancements were superior to salicylic acid or chitosan alone. Altogether, our findings promote the sustainable and eco-friendly application of nano-biostimulant in improving the quality of medicinal plants and green pest control in agroecosystems.</p></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106131"},"PeriodicalIF":4.2,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}