Pesticide Biochemistry and Physiology最新文献

{"title":"Re-exploration of phenolic compounds from natural waste rice husks: Combined synthesis of novel herbicide and evaluation of herbicidal activity","authors":"Li-xia Zhao ,&nbsp;Ruo-nan Song ,&nbsp;Jing-yi Li ,&nbsp;Yue-chun Zou ,&nbsp;Shuang Gao ,&nbsp;Ying Fu ,&nbsp;Fei Ye","doi":"10.1016/j.pestbp.2025.106312","DOIUrl":"10.1016/j.pestbp.2025.106312","url":null,"abstract":"<div><div>In a global context, weed management has emerged as a significant challenge in agricultural production, causing substantial economic losses annually. Consequently, the development of innovative and efficient herbicides is crucial. Natural products serve as important sources for discovering novel herbicides. Through chemical synthesis and structural modification of natural compounds, novel herbicides can be developed. Protoporphyrinogen oxidase (PPO, EC 1.3.3.4) catalyzes the oxidation of protoporphyrinogen IX to protoporphyrin IX, a critical step in the biosynthesis of porphyrins. PPO inhibitors target protoporphyrinogen oxidase, inhibiting its activity and thereby blocking porphyrin biosynthesis, ultimately leading to plant death. In this research, protoporphyrinogen oxidase was targeted, and monomeric compounds were extracted from natural rice husks, followed by selective manipulation to design and synthesize 33 new compounds. Weed control assays (37.5–300 g ai/ha) showed that most of the new compounds exhibited significant herbicidal activity against dicot weeds, but less effectiveness against monocot weeds. In particular, compound <strong>I–c4</strong> demonstrated excellent post-emergence herbicidal activity on six weed species, comparable to the commercial herbicide oxyfluorfen. Activity experiments showed that compound <strong>I–c4</strong> significantly reduced PPO levels in weeds. Molecular docking results indicated that compound <strong>I–c4</strong> effectively occupies the substrate pocket of PPO enzymes, demonstrating strong inhibitory effects on receptor protein activity. Thus, compound <strong>I–c4</strong> shows promise as a novel PPO herbicide for effective weed control.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106312"},"PeriodicalIF":4.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicological investigations of biosynthesized nickel ferrites nanoparticles on midgut epithelium of Blaps polychresta as nanopesticides: Structural damages and oxidative stress","authors":"Esraa A. Arafat ,&nbsp;Abdelazeem S. Eltaweil ,&nbsp;Eman M. Abd El-Monaem ,&nbsp;Hanan I. Elhenawy ,&nbsp;Hussein K. Hussein ,&nbsp;Mohamed A. Hassan ,&nbsp;Lamia M. El-Samad","doi":"10.1016/j.pestbp.2025.106314","DOIUrl":"10.1016/j.pestbp.2025.106314","url":null,"abstract":"<div><div>The development of eco-friendly insecticides with the ability to overcome insecticide resistance remains a thorny issue. This study therefore intends to evaluate the effectiveness of green synthesized nickel ferrite nanoparticles (NiFe₂O₄ NPs) as nanopesticides in this first report using darkling beetles, <em>Blaps polychresta</em>, as a model. The biosynthesized NiFe₂O₄ NPs were characterized employing various approaches, including FTIR, UV–Vis spectroscopy, XRD, XPS, and VSM. The morphological features and size of the nanoparticles were determined adopting SEM and TEM analyses, revealing that NiFe₂O₄ NPs have an average size of 24.59 nm with a quasi-spherical shape. The beetles treated with NiFe₂O₄ NPs showed their accumulation in midgut tissues, as evidenced by EDX analysis. Accumulation of NiFe₂O₄ NPs in midgut epithelium induced surplus reactive oxygen species (ROS) production, engendering elevated MDA level linked with increased expression of MT1 and HSP70. Besides, decreased GST, SOD, and GSH activities were also reported. These findings indicate antioxidant defense systems malfunction due to failure of NiFe₂O₄ NPs detoxification. Beyond that, comet assay and flow cytometry analysis exhibited noticeable amplifications of DNA impairment and cellular apoptosis, respectively, in beetles treated with NiFe₂O₄ NPs compared to untreated beetles. Pathohistological and ultrastructure investigations showed various aberrations in beetles exposed to NiFe₂O₄ NPs, including rapture of midgut epithelium and necrotic signs, substantiating the biochemical findings. These findings suggest that NiFe₂O₄ NPs could be implemented in agricultural practices as a sustainable insecticidal candidate to manage insect pests. Further studies are required to investigate NiFe₂O₄ NPs interaction with environmental conditions.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106314"},"PeriodicalIF":4.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143277381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A functional study of the trehalase genes in Tribolium castaneum and their application in the construction of RNAi engineering bacteria","authors":"Yue Li ,&nbsp;Puxing Hou ,&nbsp;Ruyu Li ,&nbsp;Pei Li ,&nbsp;Zhiqing Ma ,&nbsp;Hua Wu ,&nbsp;Zhili Jiang","doi":"10.1016/j.pestbp.2025.106315","DOIUrl":"10.1016/j.pestbp.2025.106315","url":null,"abstract":"<div><div><em>Tribolium castaneum</em>, belonging to the order Coleoptera, family Tenebrionidae, is a global grain storage pest. The enzyme trehalase can catalyze trehalose decomposition and participate in chitin synthesis, which is of great significance in insect physiology and may be a key target for <em>T. castaneum</em> pest prevention and control<em>.</em> This study focused on <em>T. castaneum</em> and explored the function of its trehalase (<em>TcTre</em>) in test insects' growth and development process. We analyzed the roles of <em>TcTre</em> in different growth stages and tissues of <em>T. castaneum</em> by measuring its spatio and temporal expression patterns. The silencing of <em>TcTre</em> by RNAi technology reduced the transcription level of the target gene, affected the enzyme activity of trehalase, disturbed the sugar balance, blocked the pathway of chitin synthesis, and caused abnormal molting and wing development of the tested insects. Key genes about pest control such as <em>TcTre1–1</em>, <em>TcTre1–3</em>, and <em>TcTre2</em> were screened, which caused the accumulated mortality of 53.33 %, 56.67 %, and 50.00 % respectively. Subsequently, an engineered bacterium, <em>Tre</em>-L4440-HT115 (DE3), was developed to efficiently express dsRNA and mediate insecticidal activity. The dsRNA produced by the bacterial solution, targeting <em>TcTre1–1</em>, <em>TcTre1–3,</em> and <em>TcTre2</em> fragments for silencing, could cause the death of 44.44 %, 48.89 %, and 46.67 % of the test insects cumulatively. This advancement was aimed at reducing the production costs of dsRNA and laying a scientific foundation for the industrial development of nucleic acid pesticides for <em>T. castaneum</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106315"},"PeriodicalIF":4.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143277408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of the controllable release and insecticidal efficacy for two typical carrier methods on diamide insecticide delivery system","authors":"Bingrui Liu,&nbsp;Yucong Huang,&nbsp;Xingyu Liu,&nbsp;Huiying Yang,&nbsp;Shaochen Li,&nbsp;Yahui Li","doi":"10.1016/j.pestbp.2025.106313","DOIUrl":"10.1016/j.pestbp.2025.106313","url":null,"abstract":"<div><div>Using nano/microcarriers of pesticides in sustainable pest management represents a promising strategy for enhancing pesticide efficiency while mitigating environmental harm. The reported pesticide loading methods include one-step self-assembly encapsulation and two-step absorption loading, but the controllable release and insecticidal efficacy of these two methods have been infrequently evaluated. Herein, the typical diamide insecticide cyantraniliprole (CTP) was employed as the model pesticide. A hydrogen bond-driven one-step self-assembly method and a chemical deposition method were utilized to fabricate highly dispersed polylactic acid (PLA) microspheres and calcium carbonate (CaCO₃) microspheres. The resulting CTP-loaded PLA microspheres (CTP-PLA MS) and CaCO₃ microspheres (CTP-CaCO₃ MS) both exhibited high adhesion, resistance to rain erosion, and insecticidal activity under laboratory conditions. However, the functional CTP-PLA MS demonstrated superior sustained pesticide release performance, higher pesticide loading capacity, and less application amount than that of CTP-CaCO₃ MS. At the same time, the acute toxicity of CTP-PLA MS exhibited slightly reduced acute toxicity to honeybees (<em>Apis mellifera</em>), signifying enhanced biocompatibility. Finally, the CTP-PLA MS maintained superior insecticidal efficacy than the normal CTP in controlling <em>O. nubilalis</em> at a low concentration. The present study represents a promising pesticide carrier as a highly efficient, eco-friendly agent for sustained management of <em>O. nubilalis</em>.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106313"},"PeriodicalIF":4.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and synthesis of sulfur-containing pseudilin analogs and their biological activity evaluation","authors":"Jili Wang ,&nbsp;Yaqing Zhou ,&nbsp;Can Chen ,&nbsp;Zhiyi Wang ,&nbsp;Xin Wu ,&nbsp;Xin Zhou ,&nbsp;Yong Sun ,&nbsp;Wenhai Wu ,&nbsp;Aidong Zhang","doi":"10.1016/j.pestbp.2025.106311","DOIUrl":"10.1016/j.pestbp.2025.106311","url":null,"abstract":"<div><div>Pseudilin is a natural product that inhibits the enzyme 4-diphosphocytidyl-2C-methyl-D-erythritol synthase (IspD) in the methylerythritol phosphate (MEP) pathway, and using it as a template for designing lead structure is an effective strategy in developing new active compounds. In this work, a series of sulfur-containing pseudilin analogs were designed by replacing the pyrrole rings in pseudilin with pyrazole rings through bioisosteric replacement strategy, while introducing sulfur-containing groups. The target compounds were synthesized through a four-step reaction sequence, in which the key intermediate of sulfur-containing chromones was obtained by cyclization reaction, further transannulation, oxidation, and finally bromination. The bioactivity evaluation demonstrated that most of the synthesized compounds exhibited certain IspD enzyme inhibitory activity and good pre-emergence model plants inhibitory activity. Notably, these compounds showed stronger inhibitory effects on the monocot <em>Echinochloa crus-galli</em> (<em>E. crus-galli</em>) compared to the dicot <em>Brassica napus L</em> (<em>B. napus L)</em>. In particular, compound <strong>5h</strong> exhibited potent inhibitory activity against the <em>Arabidopsis thaliana</em> IspD (<em>At</em>IspD) enzyme and achieved the highest pre-emergence herbicidal activity, superior to the control pentabromopseudilin (<strong>PBQ</strong>). The molecular docking results showed that compound <strong>5h</strong> could bind well to the allosteric site of <em>At</em>IspD, and the confirmed <strong>5h</strong> and <strong>PBQ</strong> bind to the IspD in a similar way. These results suggest that the sulfur-containing compound <strong>5h</strong> may represent a novel herbicide lead compound with new mode of action targeting IspD in the MEP pathway.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106311"},"PeriodicalIF":4.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effect of β-ocimene on Hyphantria cunea sex pheromone and its potential attraction mechanism related to Ca2+ stimulation","authors":"Siye Zhou ,&nbsp;Jiayun Li ,&nbsp;Dongping Chen,&nbsp;Zhixuan Xu,&nbsp;Kudousi Kuerban,&nbsp;Qiang Liu,&nbsp;Muhammad Irfan Waris,&nbsp;Wenwu Wu,&nbsp;Nannan Lv,&nbsp;Jianting Fan","doi":"10.1016/j.pestbp.2025.106310","DOIUrl":"10.1016/j.pestbp.2025.106310","url":null,"abstract":"<div><div><em>Hyphantria cunea</em>, a globally significant quarantine pest, causes severe ecological and economic impacts in invaded regions. Attractants are environment-friendly and valuable pest management tools for <em>H. cunea</em> control. In this study, we identified that the combination of β-ocimene with sex pheromone components was strongly attractive to <em>H. cunea</em> males. This attractant combination activates the Ras signaling pathway and stimulates Ca²⁺ in male adults following exposure. Feeding and oviposition behavior results demonstrated that <em>H. cunea</em> prefers <em>Morus alba</em> over <em>Platanus</em> × <em>acerifolia</em>, <em>Metasequoia glyptostroboides</em>, and <em>Taxodium distichum</em>. In the fresh branches of <em>M. alba</em> with leaves, the relative abundance of β-ocimene (36.33 ± 0.41 %) was significantly higher than the other hosts. Electroantennography recordings, behavioral choice assays, and field trapping trials showed that the combination of β-ocimene with sex pheromone components significantly enhanced male response and attraction. The number of trapped males using β-ocimene/ sex pheromone blend was 3.7-fold higher than that of sex pheromone or β-ocimene alone in the field, indicating a synergistic effect of adding β-ocimene to sex pheromone. The molecular mechanism of β-ocimene/sex pheromone synergism was further analyzed. After exposure to the attractant, the Ras signaling pathway in the heads of males are activated (<em>HcILP</em> and <em>HcPLCε1</em> are upregulated, while <em>HcRasGAP</em> and <em>HcPLD</em> are downregulated), which further stimulates the expression of IP3 and Ca²⁺. The activation of Ca²⁺ may be the key reason for its higher attraction to males. These findings provide a theoretical basis for the selection and mechanistic understanding of attractants for <em>H. cunea</em>, offering insights for attractant-based pest control strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106310"},"PeriodicalIF":4.2,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of the insecticide thiodicarb on non-target organs and behavior of the stingless bee Partamona helleri (Hymenoptera: Meliponini)","authors":"Davy Soares Gomes ,&nbsp;Franciane Rosa Miranda ,&nbsp;João Victor de Oliveira Motta ,&nbsp;Mateus Soares de Oliveira ,&nbsp;Cliver Fernandes Farder-Gomes ,&nbsp;Daniel Silva Sena Bastos ,&nbsp;Rodrigo Cupertino Bernardes ,&nbsp;Fernanda Pereira da Silva ,&nbsp;Pollyana Leão Gonçalves ,&nbsp;Hugo Bolsoni Zago ,&nbsp;Cynthia Canêdo da Silva ,&nbsp;Leandro Licursi de Oliveira ,&nbsp;Kenner Morais Fernandes ,&nbsp;José Eduardo Serrão","doi":"10.1016/j.pestbp.2025.106308","DOIUrl":"10.1016/j.pestbp.2025.106308","url":null,"abstract":"<div><div>Stingless bees are vital pollinators in the Neotropics and can be exposed to pesticides used in agriculture during their foraging activities. Among these pesticides, the insecticide thiodicarb is commonly applied to bean and sunflower crops, posing a potential risk to the stingless bee <em>Partamona helleri</em>, which pollinates these plants. This study aimed to investigate the effects of thiodicarb on mortality, morphology, programmed cell death signaling pathways in the midgut and Malpighian tubules, and the locomotion behavior of <em>P. helleri</em> workers. The estimated lethal concentration (LC₅₀) of thiodicarb for <em>P. helleri</em> was 87 μg a.i. mL⁻¹. Bees fed this concentration exhibited morphological damage to the midgut epithelium and Malpighian tubules, including brush border degeneration, increased cytoplasm vacuolation, and the release of cell fragments into the intestinal lumen. In the midgut, regenerative cells showed cytoplasm disorganization and nuclear pyknosis. Immunofluorescence analysis revealed an increase in cell death <em>via</em> autophagy and apoptosis in the epithelial cells of the affected organs. Ingestion of thiodicarb also altered the bees' locomotion, resulting in increased meandering and decreased walking speed and total distance traveled. These findings demonstrate that the LC₅₀ of thiodicarb causes damage to non-target organs as the midgut and Malpighian tubules, and impairs behavior, which may ultimately affect the pollination services provided by <em>P. helleri</em>. These results enhance our understanding of the vulnerability of stingless bees to insecticides and underscore the importance of strategies aimed at protecting these pollinators.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106308"},"PeriodicalIF":4.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative toxicity mechanisms of sulfoxaflor and lambda-cyhalothrin against Apolygus lucorum from enzymatic and transcriptomic perspectives: Efficient application of insecticides","authors":"Lu Xu ,&nbsp;Baosheng Liu ,&nbsp;Liubin Xiao ,&nbsp;Zhichun Zhang ,&nbsp;Hongtao Niu ,&nbsp;Dongxiao Zhao ,&nbsp;Shuai Sun ,&nbsp;Huifang Guo","doi":"10.1016/j.pestbp.2025.106306","DOIUrl":"10.1016/j.pestbp.2025.106306","url":null,"abstract":"<div><div>The pyrethroid insecticide lambda-cyhalothrin is threatened by insecticide resistance and has been registered to control <em>Apolygus lucorum</em>. The sulfoximine insecticide sulfoxaflor as an excellent candidate is recommended for its management. Previous studies have mainly focused on identifying resistance genes and their sublethal effects on the biological characteristics of these two insecticides in this pest. However, the toxicity mechanism differences of lambda-cyhalothrin and sulfoxaflor exposures are largely unknown. The LD₁₀ and LD₃₀ values were measured with significant difference as 0.15, 0.46, 33.58, and 73.60 ng/insect for sulfoxaflor and lambda-cyhalothrin, respectively, indicating differences in the insecticide type. Exposure to sublethal sulfoxaflor resulted in a higher total number of differentially expressed genes (DEGs) (550 and 995 DEGs) than exposure to sublethal lambda-cyhalothrin (101 and 112 DEGs). Moreover, enrichment analysis showed that more metabolic and signaling pathways were involved in the toxicity of sulfoxaflor than that of lambda-cyhalothrin, and enzyme activities in the enriched pathways were induced by sulfoxaflor and inhibited by lambda-cyhalothrin. For transcriptome validation, DEGs encoding detoxification-related genes were identified and validated by quantitative real-time PCR (qRT-PCR). These results indicate that sulfoxaflor is more toxic than lambda-cyhalothrin due to different modes of action. Our findings not only first provide insight into the toxicity mechanism differences of lambda-cyhalothrin and sulfoxaflor action and detoxification in <em>A. lucorum</em> at molecular and biochemical levels but also offer data and techniques for registering candidate sulfoxaflor and efficient application of insecticides in the field.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106306"},"PeriodicalIF":4.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143156699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The novel Bacillus thuringiensis HSY204 as a potential bioinsecticide with efficacy against Aedes aegypti larvae","authors":"Qi Lin ,&nbsp;Jiangyu Wu ,&nbsp;Xiao Feng ,&nbsp;Pan Yang ,&nbsp;Coline C. Jaworski ,&nbsp;Shakil Ahmad ,&nbsp;Wenfei Zhang","doi":"10.1016/j.pestbp.2025.106309","DOIUrl":"10.1016/j.pestbp.2025.106309","url":null,"abstract":"<div><div><em>Bacillus thuringiensis</em> (<em>Bt</em>), renowned for its potential, rapid action and environmental sustainability, remains an understudied group, particularly in newly identified strains. In this study, we screened a novel <em>Bt</em> strain, HSY204, extracted from soil samples. Our results revealed that HSY204 belonged to a new species of <em>Bt</em> with remarkable efficacy against <em>Aedes aegypti</em> larvae. The complete HSY204 genome analysis revealed nine toxin genes. In particular, a protein similar to Xpp37Aa showed very promising effectiveness in mosquito suppression, though surpassed by <em>Bacillus thuringiensis</em> subsp. <em>israelensis</em> (<em>Bti</em>). This research provides a valuable background for future biotechnological applications of HSY204 strain as a basis for the production of commercial bioinsecticides, thereby contributing to the development of innovative and environmentally friendly bioinsecticide strategies.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106309"},"PeriodicalIF":4.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effect of organometallic framework composite nanomaterial ZIF8@ZIF67 on different pathogenic microorganisms of silkworms","authors":"Zhen-Yu Shen ,&nbsp;Samreen Sadiq ,&nbsp;Tao Xu ,&nbsp;Ping Wu ,&nbsp;Iltaf Khan ,&nbsp;Xinhao Jiao ,&nbsp;Aftab Khan ,&nbsp;Lulai Wang ,&nbsp;Su Lin","doi":"10.1016/j.pestbp.2025.106307","DOIUrl":"10.1016/j.pestbp.2025.106307","url":null,"abstract":"<div><div>The domestic silkworm (<em>Bombyx mori</em>) is of considerable economic importance, but is highly susceptible to various pathogens, which leads to substantial losses in sericulture. Nanomaterials, particularly metal-organic frameworks (MOFs), have shown promise in antibacterial applications due to their broad-spectrum activity and low toxicity. This study presented the synthesis, characterization, and antibacterial evaluation of MOF-based nanomaterials, specifically ZIF8, ZIF67, and their composite ZIF8@ZIF67, for their potential as antibacterial agents against silkworm pathogens. Our findings revealed that the composite material ZIF8@ZIF67 demonstrates better antibacterial efficacy against <em>Bacillus cereus</em> and <em>Serratia marcescens</em> in vitro than pristine ZIF8 and ZIF67, with minimal inhibitory concentrations of 2.5 μg/mL and 3.0 μg/mL, respectively. Furthermore, cytotoxicity assays indicate that neither ZIF8 at 100 μg/mL nor ZIF67 and ZIF8@ZIF67 at 200 μg/mL adversely affected the viability of BmN cells. At the same time, under these concentrations, the proliferation of <em>Nosema bombycis</em> at both 48 h and 72 h post-infection was significantly inhibited. Moreover, supplementation of 300 μg/g ZIF8@ZIF67 to silkworm larvae significantly enhanced their survival rates upon infection with the bacteria above without adversely affecting silkworm growth or cocoon weight. The underlying mechanisms of action may include disruption of bacterial cell membranes, induction of oxidative stress via generation of reactive oxygen species (ROS), and initiation of apoptosis. The biocompatibility and non-toxicity of ZIF8@ZIF67 and its antibacterial efficacy suggest its potential as a safe and effective agent for silkworm disease control. Conclusively, our research offers important insights for advancing MOFs-based nanomaterials for potential antibacterial treatment in silkworms or other insects.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"208 ","pages":"Article 106307"},"PeriodicalIF":4.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143099747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}