Open Life Sciences最新文献_第3页

15-Lipoxygenase-2 deficiency induces foam cell formation that can be restored by salidroside through the inhibition of arachidonic acid effects. 15-脂氧化酶-2缺乏诱导泡沫细胞形成，红景天苷可以通过抑制花生四烯酸的作用来恢复泡沫细胞的形成。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-04-29 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1091

Rong Huang, Xi Yong, Tingting Li, Huling Wen, Xing Zhou, Yichen Liao, Jun You, Chunlei Yu, Peng Xu, Yuquan Wang, Dan Wen, Tianqin Xia, Hao Yang, Yanqin Chen, Lei Xu, Xiaorong Zhong, Xianfu Li, Zhengmin Xu, Chunyang Zhou

{"title":"15-Lipoxygenase-2 deficiency induces foam cell formation that can be restored by salidroside through the inhibition of arachidonic acid effects.","authors":"Rong Huang, Xi Yong, Tingting Li, Huling Wen, Xing Zhou, Yichen Liao, Jun You, Chunlei Yu, Peng Xu, Yuquan Wang, Dan Wen, Tianqin Xia, Hao Yang, Yanqin Chen, Lei Xu, Xiaorong Zhong, Xianfu Li, Zhengmin Xu, Chunyang Zhou","doi":"10.1515/biol-2025-1091","DOIUrl":"https://doi.org/10.1515/biol-2025-1091","url":null,"abstract":"15-Lipoxygenase-2 (15-Lox-2) is one of the key enzymes in arachidonic acid (AA) metabolic pathway, which belongs to the unsaturated fatty acid metabolic pathway. This pathway is involved in the foam cell transformation of macrophages during the progression of atherosclerosis (AS). The role of salidroside (SAL) in cardiovascular diseases has been extensively studied, but its impact on macrophage foam cell formation has not yet been clearly clarified. We aimed to determine the effects of 15-Lox-2 deficiency on macrophage (Ana-1 cell) foam cell formation, and those of SAL on 15-Lox-2-deficient macrophages. 15-Lox-2-deficient macrophages were generated using short hairpin RNA. Results indicated that 15-Lox-2 expression in the aorta of atherosclerotic patients is lower than that of the normal group. Additionally, 15-Lox-2 deficiency dramatically promoted macrophage uptake of oxidized low-density lipoprotein (ox-LDL) and increased the Cyclin D1 level while dramatically decreasing caspase3 expression. Furthermore, inflammation, complement, and TNF-α signaling pathways, along with IL1α, IL1β, IL18, and Cx3cl1, were activated in 15-Lox-2-deficient macrophages. These changes were alleviated by SAL through inhibiting AA effects, and the effects of AA on macrophages could be inhibited by SAL. Consistently, phospholipase A2-inhibitor arachidonyl trifluoromethyl ketone (AACOCF3) restored these changes. In summary, SAL reversed the effects of 15-Lox-2 deficiency on macrophages by inhibiting excessive AA and may be a promising therapeutic potential in treating atherosclerosis resulting from 15-Lox-2 deficiency.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251091"},"PeriodicalIF":1.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Ire1 gene on virulence and pathogenicity of Candida albicans. Ire1基因对白色念珠菌毒力和致病性的影响。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-04-29 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2022-1062

Huihai Zhao, Lixia Qin, Mengyan Li, Mengyu Jiang, Mengge Cui, Hua Wang, Baohua Hou, Fukun Wang, Keran Jia

{"title":"Effects of Ire1 gene on virulence and pathogenicity of Candida albicans.","authors":"Huihai Zhao, Lixia Qin, Mengyan Li, Mengyu Jiang, Mengge Cui, Hua Wang, Baohua Hou, Fukun Wang, Keran Jia","doi":"10.1515/biol-2022-1062","DOIUrl":"https://doi.org/10.1515/biol-2022-1062","url":null,"abstract":"With the extensive utilization of antifungal drugs, the drug resistance of Candida albicans is progressively intensifying, and the effect of empirical treatment for C. albicans infection is not evident. There is an urgent need for novel strategies and methods for the treatment of C. albicans infection. Our study utilized the previously constructed C. albicans Ire1 double gene deletion strain to explore the influence of the Ire1 on endoplasmic reticulum (ER) stress and pathogenicity of C. albicans through drug stress phenotype testing, biofilm and hyphomycete formation testing, and mouse systemic infection testing. The results indicate that Ire1 is involved in maintaining the integrity of the C. albicans cell wall and influencing the hyphal formation ability of C. albicans. Concurrently, the deletion of the Ire1 increased the sensitivity of C. albicans to the ER stress agents tunicamycin and dithiothreitol and diminished the biofilm formation ability of C. albicans in vitro, resulting in significant inhibition of the growth of C. albicans. In mouse models, the deletion of Ire1 completely nullified the virulence and pathogenicity of C. albicans in the tail vein infection. In conclusion, Ire1 might be a key target for the potential development of new therapeutic drugs and vaccines.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221062"},"PeriodicalIF":1.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of the PKR inhibitor, 2-aminopurine, on the replication of influenza A virus, and segment 8 mRNA splicing. PKR抑制剂2-氨基嘌呤对甲型流感病毒复制和8段mRNA剪接的影响

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1098

Dagny Lorent, Rafal Nowak, Monika Gazecka, Pawel Zmora, Elzbieta Lenartowicz Onyekaa

引用次数: 0

Optimization of phenolic compound extraction from Tunisian squash by-products: A sustainable approach for antioxidant and antibacterial applications. 突尼斯南瓜副产品酚类化合物提取工艺优化：抗氧化和抗菌应用的可持续途径。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1096

Walid Yeddes, Feten Zar Kalai, Iness Bettaieb Rebey, Majdi Hammami, Neji Tarchoun, Lilian Barros, Spyridon A Petropoulos, Hanen Falleh, Riadh Ksouri

{"title":"Optimization of phenolic compound extraction from Tunisian squash by-products: A sustainable approach for antioxidant and antibacterial applications.","authors":"Walid Yeddes, Feten Zar Kalai, Iness Bettaieb Rebey, Majdi Hammami, Neji Tarchoun, Lilian Barros, Spyridon A Petropoulos, Hanen Falleh, Riadh Ksouri","doi":"10.1515/biol-2025-1096","DOIUrl":"https://doi.org/10.1515/biol-2025-1096","url":null,"abstract":"The valorization of agricultural by-products is a key strategy for environmental sustainability. This study focuses on optimizing the extraction of phenolic compounds from by-products (peels, fibrous strands, and seeds) of two Tunisian squash landraces (e.g. Bejaoui and Karkoubi) using the response surface methodology to enhance their antioxidant and antibacterial properties. Ethanol concentration, extraction time, and temperature were the key parameters evaluated for their impact on phenolic compounds yield and bioactivity. High-performance liquid chromatography identified the major bioactive phenolic compounds, including vanillic acid, catechin gallate, hydroxytyrosol, and chlorogenic acid. The optimal extraction conditions for each by-product were defined as follows: Bejaoui peels (51.5% ethanol, 40.8°C, 50.5 min), fibrous strands (50.4% ethanol, 37.1°C, 36.3 min), and seeds (30% ethanol, 36.4°C, 8 min); Karkoubi peels (13.2% ethanol, 43.4°C, 47.2 min), fibrous strands (33.4% ethanol, 46.6°C, 10.8 min), and seeds (10.65% ethanol, 55.34°C, 23.16 min). The results demonstrated that optimizing extraction conditions may lead to significant enhancement of the total phenolic content and antiradical activity, with experimental values closely matching predictive models. Furthermore, the bioactive properties of these by-products, particularly their antibacterial activity, highlight their potential application as novel eco-friendly matrices for natural antioxidant and antimicrobial agents. This study underscores the importance of optimizing sustainable extraction techniques to maximize the valorization of agricultural waste, contributing to both environmental protection and the development of innovative natural products within the circular economy concept.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251096"},"PeriodicalIF":1.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convallatoxin inhibits proliferation and angiogenesis of glioma cells via regulating JAK/STAT3 pathway. Convallatoxin通过调控JAK/STAT3通路抑制胶质瘤细胞增殖和血管生成。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2022-1056

Zhongfei Hao, Yaming Han, Yunfei Bo, Liwen Cao, Huijie Fang, Yufei Zhang, Qingbin Li

{"title":"Convallatoxin inhibits proliferation and angiogenesis of glioma cells via regulating JAK/STAT3 pathway.","authors":"Zhongfei Hao, Yaming Han, Yunfei Bo, Liwen Cao, Huijie Fang, Yufei Zhang, Qingbin Li","doi":"10.1515/biol-2022-1056","DOIUrl":"https://doi.org/10.1515/biol-2022-1056","url":null,"abstract":"Gliomas can cause nerve cancer-related death, and surgical removal can be challenging. Convallatoxin functioned as anti-proliferation and anti-angiogenesis in cancer cells. However, convallatoxin's effect on glioma remains unclear. The aim of this study is to investigate the effect of convallatoxin on the proliferation and angiogenesis of glioma cells, and explore the underlying mechanism. Human glioma cell lines U251MG and A172 were treated with 12.5, 25, and 50 nM convallatoxin. Cell proliferation was investigated using the CCK-8 assay and colony formation assay. Migration and invasion were analyzed with transwell assays. Angiogenesis was evaluated using a tube formation assay. The phosphorylation of Janus kinase (JAK) and signal transducer and activator of transcription 3 (STAT3) was measured using Western blots. A xenotransplantation model of nude mice was used to investigate glioma progression. In U251MG and A172 cells, convallatoxin dose-dependently reduced cell viability and colony formation. Convallatoxin suppressed migration and invasion. Similarly, convallatoxin-treated cells had weakened angiogenesis. Convallatoxin downregulated JAK and STAT3 phosphorylation levels. Convallatoxin also inhibited glioma progression in nude mice xenotransplantation models. By inhibiting the JAK/STAT3 signaling pathway, convallatoxin inhibited proliferation, migration, invasion, and angiogenesis of glioma cells, proving to be a promising therapeutic candidate for gliomas.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221056"},"PeriodicalIF":1.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Indices of human impacts on landscapes: How do they reflect the proportions of natural habitats? 人类活动对景观影响的指数：如何反映自然生境的比例？

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1085

Markéta Šantrůčková, Katarína Demková, Tomáš Frantík, Jiří Dostálek

{"title":"Indices of human impacts on landscapes: How do they reflect the proportions of natural habitats?","authors":"Markéta Šantrůčková, Katarína Demková, Tomáš Frantík, Jiří Dostálek","doi":"10.1515/biol-2025-1085","DOIUrl":"https://doi.org/10.1515/biol-2025-1085","url":null,"abstract":"Human activities significantly influence landscapes, altering natural habitats and ecosystem services. This study examines the relationship between human impacts, measured by the hemeroby index and coefficient of anthropogenic impact (CAI´), and the presence of natural habitats in the Czech Republic. Using CORINE land cover data and natural habitat mapping, we analysed national and regional scales to assess the effectiveness of these indicators in reflecting environmental changes. Compared with the simple anthropogenic impact coefficient (CAI´), the hemeroby index, which accounts for both the quantity and quality of ecosystems, provides more detailed insights. At the national level, both indices had an equally close relationship with the proportion of natural habitats, but at the regional level, the results for the hemeroby index were better. Our findings indicate a strong negative correlation between human impacts and the proportion of natural habitats, emphasizing the importance of refined indicators for environmental monitoring and policy-making. The advantage of both indices is that they could be easily calculated from satellite images and/or land cover data. Therefore, they could be used worldwide.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251085"},"PeriodicalIF":1.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sodium butyrate aids brain injury repair in neonatal rats. 丁酸钠对新生大鼠脑损伤的修复作用。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-04-25 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2022-1046

Jing Zhao, Jun Zhang, Can Yang, Linlin Yin, Li Hou, Lin Jiang

{"title":"Sodium butyrate aids brain injury repair in neonatal rats.","authors":"Jing Zhao, Jun Zhang, Can Yang, Linlin Yin, Li Hou, Lin Jiang","doi":"10.1515/biol-2022-1046","DOIUrl":"https://doi.org/10.1515/biol-2022-1046","url":null,"abstract":"The aim of this study is to investigate the effects and mechanism of action of sodium butyrate (SB) on brain injury repair in neonatal rats. 126 neonatal SD rats were randomly allocated to 7 groups, and necrotizing enterocolitis (NEC) and hypoxic-ischemic brain injury (HIBI) rat models were established. Hematoxylin and eosin staining showed that SB intervention alleviated intestinal and brain injuries in the HIBI + SB, NEC + SB, and NEC + HIBI + SB groups. Compared to the NEC and NEC + HIBI groups, the NEC + SB and NEC + HIBI + SB groups had significantly higher interleukin (IL)-10 and lower IL-17 levels (P < 0.05). Immunohistochemistry revealed increased Bcl-2 expression and decreased Bax expression in the NEC + SB and NEC + HIBI + SB groups compared to the NEC and NEC + HIBI groups in intestinal and brain tissues (P < 0.05). Compared to the control group (CG), gut microbiota diversity decreased in the HIBI, NEC, and NEC + HIBI groups, and increased significantly in the HIBI + SB, NEC + SB, and NEC + HIBI + SB groups. SB may alleviate brain injury by modulating gut microbiota, affecting IL-10 and IL-17 levels, and regulating Bcl-2 and Bax expression in intestinal and brain tissues.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221046"},"PeriodicalIF":1.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12032984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sirt1 protects lupus nephritis by inhibiting the NLRP3 signaling pathway in human glomerular mesangial cells. Sirt1通过抑制人肾小球系膜细胞NLRP3信号通路保护狼疮性肾炎。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-04-25 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2022-1038

Yu Zhao, Ai-Ping Zhang, Bei-Yan Bao, Heng Fan, Xu-Yan Yang

{"title":"Sirt1 protects lupus nephritis by inhibiting the NLRP3 signaling pathway in human glomerular mesangial cells.","authors":"Yu Zhao, Ai-Ping Zhang, Bei-Yan Bao, Heng Fan, Xu-Yan Yang","doi":"10.1515/biol-2022-1038","DOIUrl":"https://doi.org/10.1515/biol-2022-1038","url":null,"abstract":"Lupus nephritis (LN) is the most common and lethal complication of systemic lupus erythematosus. We aimed to explore the protective effect of Sirtuin1 (Sirt1) on LN by regulating the NLRP3 signaling pathway in human glomerular mesangial cells (GMCs). We collected clinical samples from patients with LN, detected Sirt1 protein and mRNA expression using biochemical methods, cultured GMCs in vitro, evaluated levels of oxidative stress, cell apoptosis, and mitochondrial damage, and analyzed the expression of NLRP3 pathway proteins. Our results demonstrated that Sirt1 protein and mRNA were downregulated in the renal tissue of LN patients, and LN serum induced an increase in oxidative stress, cell apoptosis, and mitochondrial damage in GMCs while activating the NLRP3 signaling pathway. Upregulation of Sirt1 inhibited LN serum-induced oxidative stress in GMCs, reduced the number of cell apoptosis, and stabilized mitochondrial structure and function. Moreover, Sirt1 overexpression inhibited the expression of NLRP3 pathway proteins. Our findings suggest that Sirt1 may protect LN by inhibiting the NLRP3 signaling pathway in GMCs.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221038"},"PeriodicalIF":1.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12032988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased serum TIMP4 levels in patients with rheumatoid arthritis. 类风湿关节炎患者血清TIMP4水平降低。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-04-25 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2022-1037

Jinyu Chen, Yanyan Fan, Shengyu Cui, Haiping Zhang, Ziliang Yu, Yali Jiang, Xiaogang Zhou

{"title":"Decreased serum TIMP4 levels in patients with rheumatoid arthritis.","authors":"Jinyu Chen, Yanyan Fan, Shengyu Cui, Haiping Zhang, Ziliang Yu, Yali Jiang, Xiaogang Zhou","doi":"10.1515/biol-2022-1037","DOIUrl":"https://doi.org/10.1515/biol-2022-1037","url":null,"abstract":"The current study was designed to explore the clinical significance of serum tissue inhibitor of metalloproteinase 4 (TIMP4) levels in rheumatoid arthritis (RA). The GSE1919 chip was analyzed, differentially expressed genes (DEGs) were identified, and gene ontology as well as Kyoto Encyclopedia of Genes and Genomes analyses of the identified DEGs were conducted. Patients with RA (n = 96) and healthy individuals (n = 96) were enrolled in this study. Serum from the participants was collected, and RT-qPCR as well as WB have been conducted to examine TIMP4 levels; additionally, interleukin (IL)-6 and IL-1β levels were determined using the ELISA method. Pearson's correlation analysis was conducted for evaluating relationships between the expression levels of TIMP4 and those of IL-6 or IL-1β. A receiver operating characteristic (ROC) curve was drawn to determine the potential diagnostic value of serum TIMP4 for RA. TIMP4 was identified as a markedly downregulated gene involved in RA development. TIMP4 levels were significantly decreased in patients with RA, and the results of the ROC analysis showed that TIMP4 may be a potential diagnostic marker. Furthermore, the concentrations of IL-6 and IL-1β were markedly elevated in patients with RA. Finally, TIMP4 levels showed negative correlation with the levels of either IL-6 or IL-1β. TIMP4 is downregulated in RA and is a reliable serum marker for RA diagnosis.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221037"},"PeriodicalIF":1.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12032977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction of MTHFR polymorphism with PAX1 methylation in cervical cancer. 宫颈癌中MTHFR多态性与PAX1甲基化的相互作用。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-04-25 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2022-1052

Xiao-Yan Zhou, Meng-Meng Chen, Jun-Mei Yu, Yang Zhou, Yan-Song Luan, Bing-Qiang Zhang, Yun-Yuan Zhang

{"title":"Interaction of MTHFR polymorphism with PAX1 methylation in cervical cancer.","authors":"Xiao-Yan Zhou, Meng-Meng Chen, Jun-Mei Yu, Yang Zhou, Yan-Song Luan, Bing-Qiang Zhang, Yun-Yuan Zhang","doi":"10.1515/biol-2022-1052","DOIUrl":"https://doi.org/10.1515/biol-2022-1052","url":null,"abstract":"We aimed to investigate the roles and interaction effects of high-risk human papillomavirus (HR-HPV) infection, methyltetrahydrofolate reductase (MTHFR) polymorphism, and paired box gene 1 (PAX1) methylation in cervical intraepithelial neoplasia (CIN) and cervical cancer. Polymerase chain reaction was used to detect MTHFR polymorphism and PAX1 methylation; Mantel-Haenszel and Spearman's rank correlation tests were used to analyze the trends and correlations. Forty cases each of normal control (NC), CIN I, and CIN II/III and 9 squamous cell carcinoma (SCC) cases were enrolled. Increase in age increases the risk of cervical cancer. The HR-HPV infection rate, MTHFR mutation rate, and PAX1 methylation rate in CIN I, CIN II/III, and SCC groups were significantly higher than those in the NC group (P < 0.05). The above-mentioned rates gradually increased with the degree of cervical lesions. Moreover, HR-HPV infection, MTHFR polymorphism, and PAX1 methylation increased the risk of both CIN and cancer. A positive additive interaction was observed between PAX1 methylation and MTHFR polymorphism across different cervical lesion groups, whereas no interaction was found between HR-HPV infection and PAX1 methylation in lesion progression.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221052"},"PeriodicalIF":1.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12032982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0