Open Life Sciences最新文献

{"title":"Preparation and characterization of lemongrass oil nanoemulsion: Antimicrobial, antibiofilm, antioxidant, and anticancer activities.","authors":"Mohamed T Selim, Bahaa M Badr, Salem S Salem, Fathy M Elkady, Mostafa A Abdel-Maksoud, Nasser Ibrahim Issa, Karim M Sobhy, Khaled M Shaban, Ahmed A Abdallah, Ali M Sabeq, Abdulaziz Alamri, Mohamed Y Zaky, Abeer S Aloufi, Amr H Hashem","doi":"10.1515/biol-2025-1159","DOIUrl":"10.1515/biol-2025-1159","url":null,"abstract":"<p><p>Although citrus essential oils, including lemongrass essential oil, have antibacterial, anti-biofilm, and antioxidant properties, their biological instability and poor water solubility render them unsuitable for industrial usage. Thus, this study aimed to prepare both lemongrass essential oil emulsion (LEO-E) and lemongrass essential oil nanoemulsion (LEO-NE), and evaluate their different bioactivities. Characterization by gas chromatography-mass spectroscopy (GC-MS) and evaluation of antimicrobial, antibiofilm, antioxidant, and anticancer activities were carried out. GC-MS results illustrated that D-limonene compound is the dominant among other compounds in LEO. According to transmission electron microscopy and dynamic light scattering, LEO-NE appeared as spherical-shaped droplets with a constant size spanning from 29.1 to 37.4 nm with a polydispersity index value of 0.163. Antimicrobial results showed that LEO-NE exhibited promising antimicrobial activity against <i>Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans</i>, and <i>Austromerope brasiliensis</i> with inhibition zones of 25.33 ± 1.1, 26.5 ± 0.5, 22 ± 1, 24.33 ± 0.5, 28.6 ± 1, and 27.97 ± 0.9 mm, respectively. Moreover, LEO-NE showed considerable antiـbiofilm efficacy toward <i>S. aureus</i> and <i>P. aeruginosa</i> with inhibition percentages at 1/2 of MIC of 98.92 and 92.62%, respectively. Furthermore, LEO-NE exhibited antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl method with 88.5% at 100 µg/mL concentration. In addition, LEO-NE displayed potential anticancer activity toward the human prostate cancer cell line (PC3) and human liver cancer cell line (Hep-G2), where IC₅₀ values were 170.09 and 105.06%, respectively. In conclusion, the prepared LEO-NE in the current study had antimicrobial, antibiofilm, antioxidant, and anticancer activities, which can be used in the medical and pharmaceutical fields.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251159"},"PeriodicalIF":1.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental study on salmon demineralized bone matrix loaded with recombinant human bone morphogenetic protein-2: <i>In vitro</i> and <i>in vivo</i> study.","authors":"Lei Wang, Jing Zhu, Xiaorui Jiang, Shouyi Li","doi":"10.1515/biol-2025-1152","DOIUrl":"10.1515/biol-2025-1152","url":null,"abstract":"<p><p>This article aims to explore the effects of salmon demineralized bone matrix (DBM) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) on bone formation. Salmon DBM, with its high water absorption capacity, was used to construct a composite material with rhBMP-2 under pH 7.0 and optimal temperature conditions. The compound effects of the composite material were evaluated by measuring its mechanical strength, microstructure, and biocompatibility through scanning electron microscopy and cell culture experiments. The effect of rhBMP-2 within the composite was assessed by <i>in vivo</i> fluorescence imaging. rhBMP-2 was successfully loaded onto salmon DBM and released slowly. The composite material's structure, strength, and cell compatibility were unaffected. The compressive strength was 2.87 MPa, slightly higher than DBM alone (2.27 MPa). <i>In vivo</i> imaging showed slow release of rhBMP-2, with more than 50% fluorescence intensity remaining after 3 days. Cytotoxicity tests showed no harmful effects, with over 95% cell growth. Rats with rhBMP-2-loaded DBM had higher serum calcium (1,569 ± 114 mg/L) than those with DBM alone (1,349 ± 110 mg/L, <i>p</i> < 0.05). Histology showed more bone growth and calcium deposition around rhBMP-2-loaded DBM. Loading rhBMP-2 onto DBM does not alter its physical, chemical, or biological properties; it enhances the osteogenic potential of DBM.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251152"},"PeriodicalIF":1.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of IgA nephropathy treated with a combination of telitacicept and half-dose glucocorticoids.","authors":"Suojian Zhang, Di Yin, Qin Xu, Caixia Zhao, Jianmei Sha, Zhenguo Qiao, Juan Cao","doi":"10.1515/biol-2025-1158","DOIUrl":"10.1515/biol-2025-1158","url":null,"abstract":"<p><p>Immunoglobulin A nephropathy (IgAN) is the most common primary glomerular disease in China; there is an urgent need to identify more effective treatments for IgAN. A 34-year-old woman presented with proteinuria of >2 years' duration. She was diagnosed with IgA nephropathy and was treated with a combination of telitacicept and half-dose glucocorticoids. At the time of writing, the patient's urinary protein concentration and renal function were normal. A combination of telitacicept and half-dose glucocorticoids is an effective way to treat IgAN.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251158"},"PeriodicalIF":1.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometric analysis and immunobiological investigation of <i>Indigofera oblongifolia</i> on the infected lung with <i>Plasmodium chabaudi</i>.","authors":"Mutee Murshed, Jameel Al-Tamimi, Hossam Ebaid, Saleh Al-Quraishy","doi":"10.1515/biol-2025-1110","DOIUrl":"10.1515/biol-2025-1110","url":null,"abstract":"<p><p>The present study aimed to evaluate the therapeutic potential of <i>Indigofera oblongifolia</i> with silver nanoparticles (AgNPs) and chloroquine (CQ) 10 mg/kg in treating lung inflammation caused by <i>Plasmodium chabaudi</i> infection in a mouse model. Fifty female C57BL/6 mice were divided into five groups: control, <i>Indigofera oblongifolia</i> leaf extract (IOLE) AgNPs treated, <i>P. chabaudi</i> infected, infected and IOLE AgNPs treated, infected and CQ 10 mg/kg treated. Lung histopathology was assessed using microscopic analysis and immunohistochemistry investigation for TNF-α and IL-6. The results showed that the positive control of AgNPs slightly triggered proinflammatory cytokines and created an oxidative stress status in lung tissue. The group IOLE AgNPs treatment significantly restored the normal organization of the control lung tissue. It reduced alveolar and septal congestion, edema, and necrosis compared to the infected lung. Therefore <i>I. oblongifolia</i> as a natural medical plant displayed significant antimalarial and anti-oxidant properties effectively, reducing inflammatory signs and cytokine levels in <i>P. chabaudi</i>-infected lungs and treating the harmful impact of AgNPs in <i>P. chabaudi</i>-infected + <i>I. oblongifolia</i> with AgNPs lung. While CQ shows limited efficiency, it showed moderate improvement in the histological architecture such as thicker alveolar and bronchiolar walls and restricted expansion. However, the septal and alveolar congestion, hemosiderin concentration, edema, and necrotic cells were still present. Also, immunohistochemistry expression of proinflammatory cytokines is still expressed. In conclusion, this study highlights the therapeutic potential of <i>I. oblongifolia</i> for malaria management. Also, this study uniquely explored the combined influences of <i>I. oblongifolia</i> leaf extract and AgNPs on lung inflammation caused by <i>P. chabaudi</i> infection. Previous studies may have explored these components separately, but the current study examines their synergistic potential in treating malaria-related lung pathology. Consequently, the study compared the efficacy of <i>I. oblongifolia</i> with that of CQ, revealing that the latter exhibited limited efficiency due to drug resistance and its inability to restore the normal features of its histology. This comparison highlights the potential impact <i>of I. oblongifolia</i> as a more effective alternative in malaria treatment, particularly in cases where conventional drugs fail.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251110"},"PeriodicalIF":1.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The NuA4/TIP60 histone-modifying complex and Hr78 modulate the <i>Lobe</i> ^<i>2</i> mutant eye phenotype.","authors":"Madison P Stonbraker, Dominic A DePaul, Ethan C Heidel-Roberts, Hallee Greene, Matthew Logan Johnson","doi":"10.1515/biol-2025-1164","DOIUrl":"10.1515/biol-2025-1164","url":null,"abstract":"<p><p>Gene regulation is important during tissue formation, but redundant systems make it difficult to study <i>in vivo.</i> The protein Jazf-1 is a member of the NuA4/TIP60 histone-modifying complex, and a transcriptional repressor has been suggested to be important for <i>Drosophila melanogaster</i> eye development. We used the GAL4-UAS system to determine the impact of altering gene expression. GAL4-UAS manipulations of Jazf-1 in the eye caused variable and not fully penetrant phenotypes. Increased expression of Jazf-1 has been shown to suppress a <i>Lobe</i> ^<i>2</i> small eye phenotype. We found that <i>Lobe</i> ^<i>2</i> produces a sensitive background for an <i>in vivo</i> assay to monitor gene regulatory complexes. Depleting Jazf-1 and other NuA4/TIP60 complex members significantly enhanced the eye phenotype. We also tested Hr78, which directly interacts with Jazf-1, and found it inversely modifies the <i>Lobe</i> ^<i>2</i> phenotype. An Hr78 mutation predicted to uncouple the Jazf-1 interaction but still capable of interactions with transcriptional activators further enhanced the <i>Lobe</i> ^<i>2</i> mutant phenotype, suggesting the loss of a repressing complex. We believe that Hr78 is acting as an anchor for repressing and activating complexes and the NuA4/TIP60 complex helps repress genes that can negatively impact eye formation in the context of <i>Lobe</i> ^<i>2</i> .</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251164"},"PeriodicalIF":1.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The DREB7 transcription factor enhances salt tolerance in soybean plants under salt stress.","authors":"Giang Thu Nguyen, Yen Thi Hai Nguyen, Hung Duc Nguyen, Mau Hoang Chu, Quan Huu Nguyen","doi":"10.1515/biol-2025-1153","DOIUrl":"10.1515/biol-2025-1153","url":null,"abstract":"<p><p>DREB7 in <i>Glycine max</i> (L) is a novel trans-acting transcription factor (TF) that binds to the <i>cis</i>-acting sequences of promoters to activate the expression of downstream genes in response to abiotic factors. This study presents the experimental results and analyzes the relationship between the overexpression of the <i>GmDREB7</i> and <i>GmP5CS</i>, as well as the proline content, in transgenic soybean lines. The results of qRT-PCR analysis of four TG1 transgenic soybean lines (TG1-2, TG1-5, TG1-7, and TG1-10) showed that the <i>GmDREB7</i> gene had significantly higher transcriptional expression under untreated and salt stress conditions. Under salt stress conditions, the two transgenic lines, TG1-5 and TG1-10, had the most significant increase in <i>GmDREB7</i> and <i>GmP5CS</i> gene expression levels, as well as the highest proline accumulation (<i>P</i> < 0.05). The <i>in silico</i> molecular docking analysis confirmed a specific interaction between the DREB7 protein and GmP5CS promoter. These findings demonstrate that overexpression of the gene encoding the TF DREB7 enhanced the transcription of the <i>GmP5CS</i> gene and increased proline accumulation in soybean plants under salt stress conditions. The <i>GmDREB7</i> gene can be a promising candidate for enhancing salt tolerance in soybeans.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251153"},"PeriodicalIF":1.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and environmental drivers of <i>Suillus</i> communities in <i>Pinus sylvestris</i> var. <i>mongolica</i> forests of Inner Mongolia.","authors":"Rui-Xia Liu, You-Han Wu, Cong Li, Yi-Hua Qiao, Yi-Wen Yang, Wei-Ping Yan, Qing-Zhi Yao","doi":"10.1515/biol-2025-1156","DOIUrl":"10.1515/biol-2025-1156","url":null,"abstract":"<p><p>This study investigates the diversity and distribution of <i>Suillus</i> fungi in <i>Pinus sylvestris</i> var. <i>mongolica</i> (PSM) forests across Inner Mongolia, with a focus on understanding the environmental factors influencing fungal communities. High-throughput sequencing was utilized to analyze soil fungal communities across 12 PSM forest sites, alongside assessments of meteorological variables and soil enzyme activities. Thirteen <i>Suillus</i> species were identified, with <i>S. clintonianus</i> being the dominant species. The diversity of <i>Suillus</i> fungi exhibited significant geographical variation, with diversity decreasing from east to west. Precipitation and leucine aminopeptidase activity were identified as key drivers of fungal distribution. The soil fungal community was predominantly saprotrophic, playing a crucial role in nutrient cycling and ecosystem stability. The findings provide a deeper understanding of the role of ectomycorrhizal fungi in sustaining forest health and offer valuable insights for sustainable forest management and restoration efforts in semi-arid regions.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251156"},"PeriodicalIF":1.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solitary pulmonary metastasis with cystic airspaces in colon cancer: A rare case report.","authors":"Kunliang Guo, Yang Li, Jian Chen","doi":"10.1515/biol-2025-1149","DOIUrl":"10.1515/biol-2025-1149","url":null,"abstract":"<p><p>This report presents a case of solitary pulmonary metastasis from colon cancer, characterized by cystic airspaces, which can mimic a second primary lung cancer (LC). Preoperative contrast-enhanced computed tomography in a patient with colon cancer revealed a pulmonary micronodule with a cystic cavity in the right upper lobe. The patient subsequently underwent left-sided hemicolectomy followed by six cycles of chemotherapy. During follow-up, computed tomography demonstrated resolution of the right upper lobe nodule. However, 36 months after chemotherapy, a solid nodule with cystic airspaces appeared in the right upper lobe, exhibiting marginal spiculation, traversing vessels, and enhancement, suggestive of cystic LC. Intraoperative frozen section analysis indicated features of intestinal adenocarcinoma. A right upper lobe wedge resection was performed, and postoperative histopathology confirmed the lesion as metastatic colon adenocarcinoma with cystic airspaces. Solitary pulmonary metastasis from colon cancer is relatively uncommon, particularly with cystic presentation. Clinicians and radiologists should maintain heightened suspicion in such atypical cases to avoid missed or delayed diagnoses.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251149"},"PeriodicalIF":1.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RUNX1 promotes denervation-induced muscle atrophy by activating the JUNB/NF-κB pathway and driving M1 macrophage polarization.","authors":"Wei Hu, Yang Huang, Wei Yin, Yao Huang, Jian Wu","doi":"10.1515/biol-2025-1157","DOIUrl":"10.1515/biol-2025-1157","url":null,"abstract":"<p><p>Peripheral nerve injury-induced muscle atrophy is characterized by chronic inflammation and dysregulated macrophage polarization. RUNX1, a transcription factor upregulated in denervated muscle, has been implicated in linking muscle degeneration to inflammatory processes, but its downstream targets and mechanisms remain unclear. The aim of this study is to delineate the RUNX1-JUNB-NF-κB axis in driving inflammation-mediated muscle atrophy. The GSE183802 single-nucleus RNA sequencing dataset was analyzed to identify RUNX1-associated pathways. A sciatic nerve transection model in mice was established to validate RUNX1 expression dynamics. Chromatin immunoprecipitation, dual-luciferase reporter assays, and siRNA-mediated knockdown were used to confirm RUNX1's transcriptional regulation of <i>JUNB</i>. <i>In vitro</i> models (C2C12 myotubes, RAW 264.7 macrophages) assessed RUNX1-driven inflammatory responses, NF-κB activation, and extracellular matrix remodeling. RUNX1 was significantly upregulated in denervated muscle, particularly in myonuclei and macrophage subclusters, correlating with elevated atrophy markers (MuRF1, Atrogin-1). RUNX1 overexpression directly activated <i>JUNB</i> transcription via promoter binding, leading to NF-κB pathway activation (increased p65 phosphorylation) and M1 macrophage polarization (enhanced IL-1β/TNF-α secretion). JUNB knockdown reversed RUNX1-induced pro-inflammatory cytokine release, NF-κB signaling, and muscle atrophy markers. This study identifies the RUNX1-JUNB-NF-κB axis as a central regulator of inflammation-driven muscle atrophy following denervation. Targeting this pathway may offer therapeutic potential to mitigate neurogenic muscle degeneration and immune-mediated damage in conditions such as peripheral nerve injuries or motor neuron diseases.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251157"},"PeriodicalIF":1.7,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulmonary cryptococcosis with headache as the first presentation: A case report.","authors":"Ting Xu, Shuai Luo","doi":"10.1515/biol-2025-1119","DOIUrl":"10.1515/biol-2025-1119","url":null,"abstract":"<p><p>Pulmonary cryptococcosis (PC) is an invasive fungal infection caused by <i>Cryptococcus neoformans</i> or <i>Cryptococcus gattii</i>. Its clinical presentation and radiological findings are often non-specific, making early diagnosis challenging. Herein, the case of a 44-year-old male who presented with dizziness and headache is reported. Initial cranial magnetic resonance imaging and chest computed tomography (plain and contrast-enhanced) suggested lung cancer with brain metastasis. A definitive diagnosis was established only after a lung mass biopsy, followed by a cytological smear and histopathological analysis, confirmed PC. The patient was treated with antifungal therapy postoperatively and responded well. This case underscores the importance of considering PC in differential diagnoses to enable prompt diagnosis and treatment, potentially reducing associated mortality.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251119"},"PeriodicalIF":1.7,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}