Open Life Sciences最新文献

{"title":"Investigating context-specific acoustic variation in the bleats of male domestic sheep.","authors":"Apostolos Ntairis, Anastasia Frantzola, Ioannis Mavrikos, George P Laliotis","doi":"10.1515/biol-2025-1368","DOIUrl":"10.1515/biol-2025-1368","url":null,"abstract":"<p><p>Vocalizations provide an informative means of assessing the emotional states of animals, yet male vocal behaviour in domestic sheep remains unexplored. The study aimed to investigate whether rams' bleats vary across five distinct contexts associated with morning and night isolation, exposure to familiar ewe-bell sounds, feed anticipation, and feed denial. A total of 428 high-quality bleats were used to extract 20 acoustic parameters, which were then analyzed using a multivariate acoustic approach. Principal Component Analysis identified five independent acoustic components reflecting source-parameters, spectral energy, formant frequencies, voice stability, and temporal characteristics. Context significantly influenced the acoustic structure of bleats, with spectral, formant, vocal stability, and temporal characteristics showing context-dependent variation, whereas fundamental frequency-related parameters remained unaffected. Feed anticipation elicited higher formant-related scores; feed denial produced reduced spectral energy, and social isolation contexts were characterized by increased vocal instability. Discriminant analyses revealed moderate classification accuracy, indicating overlapping acoustic patterns and supporting graded rather than discrete vocal signals. Morphometric effects were minimal, with wither height affecting formant structure. The findings provide the first detailed evidence that rams modulate multiple acoustic features across contexts, demonstrating that male sheep encode emotional states through shifts in vocal parameter variation.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251368"},"PeriodicalIF":2.3,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myeloid sarcoma as EM blast crisis in BCR-ABL1 e6a2+ CML with myelofibrosis and TB: a case report.","authors":"Chunling Gu, Rui Lu, Yixin Kang, Wen Chen, Yongqing Zhang, Sainan Yang","doi":"10.1515/biol-2025-1369","DOIUrl":"10.1515/biol-2025-1369","url":null,"abstract":"<p><p>BCR-ABL1 e6a2-positive chronic myeloid leukemia (CML) is a rare molecular subtype of CML, accounting for less than 1 % of CML cases. It was shown to be associated with aggressive clinical behavior and a tendency toward disease progression. Here, we report a 56-year-old man who presented with pancytopenia, cervical lymphadenopathy, and severe myelofibrosis, without the typical leukocytosis observed in classic CML. Concurrent active pulmonary tuberculosis further complicated the diagnostic process. Hyperuricemia and elevated levels of lactate dehydrogenase (LDH) suggested a high cell-turnover state, and cervical lymph node biopsy confirmed myeloid sarcoma. Cytogenetic analysis revealed t(9;22)(q34;q11.2) with trisomy 8. Real-time quantitative polymerase chain reaction (RT-qPCR) identified the rare BCR-ABL1 e6a2 transcript. The patient received second- and third-generation tyrosine kinase inhibitor (TKI)-based therapy combined with chemotherapy. An individualized rifamycin-sparing anti-tuberculosis regimen was also prescribed. The patient achieved complete remission with marked regression of myelofibrosis. This case suggests that e6a2-positive CML may present with atypical features, including pancytopenia, severe myelofibrosis, and extramedullary blast crisis. In patients with concurrent tuberculosis, timely tissue biopsy and molecular testing are essential to accelerate diagnosis.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251369"},"PeriodicalIF":2.3,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13524128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148851223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AtZIK4, a MAPK kinase kinase, positively regulates phosphate uptake gene expression to modulate phosphate starvation tolerance in <i>Arabidopsis</i>.","authors":"Hongying Deng, Qian Yang, Shirong Zhao, Xuyang Lei, Liye Zhu","doi":"10.1515/biol-2025-1361","DOIUrl":"10.1515/biol-2025-1361","url":null,"abstract":"<p><p>The mitogen-activated protein kinase (MAPK) cascade is a highly conserved signal transduction module in eukaryotes. We investigated the function of ZIK4, a MAPK kinase, in the phosphate starvation response of Arabidopsis. Phenotypic analysis revealed that the <i>zik4</i> mutant had stronger tolerance to phosphate starvation than that to the wild type. However, the kinase domain was still expressed in <i>zik4</i>. The CRISPR/Cas9 system was used to construct two independent knockout mutants <i>zik4-c3</i> and <i>zik4-c15</i> by targeting two different sites in the AtZIK4 kinase domain. Physiological measurements showed that the phosphate content in <i>zik4-c3</i> and <i>zik4-c15</i> was lower than that in the wild type. Expression pattern analysis revealed that ZIK4 was induced to express under phosphate starvation. Quantitative real-time PCR results showed that the expression levels of multiple key phosphate uptake-related genes were significantly up-regulated in the <i>zik4</i> mutant. These results indicate that ZIK4, as a phosphate starvation-induced MAPKKK, promotes phosphate accumulation in Arabidopsis seedlings by upregulating the expression levels of phosphate uptake-related genes. These findings not only elucidate the regulatory role of AtZIK4 kinase during abiotic stress and expand the low-phosphate (LP) signaling cascade in <i>Arabidopsis thaliana</i>, but also providing promising genetic targets to enhance crop performance under phosphorus-limited conditions.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251361"},"PeriodicalIF":2.3,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13512907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148831200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schisandrin A alleviates allergic rhinitis by restoring Th1/Th2 and Treg/Th17 immune balance.","authors":"Zhipeng Zhang, Shan He, Yanmin Yu, Yongjun Deng, Yuan Chen, Xiaoyi Gu, Xiaoyi Qiu, Jingjing Wei, Yan Shi, Yu Jiao","doi":"10.1515/biol-2025-1348","DOIUrl":"10.1515/biol-2025-1348","url":null,"abstract":"<p><p>Allergic rhinitis (AR) is a common chronic inflammatory disorder characterized by nasal mucosal inflammation, sneezing, and rhinorrhea. Schisandrin A (Schis-A) has demonstrated significant anti-inflammatory effects in models of allergic asthma. This study aimed to evaluate the therapeutic efficacy of Schis-A in AR and to elucidate its underlying molecular mechanisms. An AR mouse model was established by sensitization and challenge with ovalbumin (OVA), followed by oral administration of Schis-A at doses of 40 and 80 mg/kg/day. Nasal symptoms were monitored and quantified. OVA-specific immunoglobulin E (IgE) levels and T helper (Th)1/Th2- and regulatory T cells (Treg)/Th17-associated cytokines were measured using enzyme-linked immunosorbent assay and quantitative real-time PCR. Treg and Th17 cell populations were analyzed by flow cytometry. The results showed that Schis-A treatment significantly reduced OVA-induced nasal rubbing and sneezing, indicating effective attenuation of allergic responses. Serum OVA-specific IgE levels were markedly reduced in Schis-A-treated AR mice. Moreover, Schis-A restored Th1/Th2 and Treg/Th17 cytokine balance, as indicated by upregulated expression of IFN-γ, IL-2, and Foxp3, alongside downregulation of IL-4, IL-5, IL-13, and IL-17. Flow cytometric analysis confirmed an increase in Treg cells and a reduction in Th17 cell populations. Mechanistically, Schis-A inhibited activation of the PI3K/AKT signaling pathway, suggesting that this pathway may contribute to its immunomodulatory effects. Schis-A alleviates AR by modulating Th1/Th2 and Treg/Th17 immune responses and inhibiting PI3K/AKT pathway activation, offering a promising therapeutic strategy for the treatment of AR.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251348"},"PeriodicalIF":2.3,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13527562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect and mechanism of SIRT1 under stress-induced vascular senescence.","authors":"Kexin Wang, Kejin Tang, Caixia Liu, Panpan Zhou, Wang He, Ying Xie, Changqing Deng","doi":"10.1515/biol-2025-1366","DOIUrl":"10.1515/biol-2025-1366","url":null,"abstract":"<p><p>A major focus of contemporary medical research is the primary pathological cardiovascular diseases, whereas stress-induced vascular senescence is a process with degenerative changes in vascular morphology, structure, and function caused by oxidative stress (OS), inflammation, autophagy disorders, and other factors. Silent information regulator of transcription 1 (SIRT1), which may be a key gene linking oxidative stress and aging, has extensive biological functions in anti-oxidation, anti-inflammation, anti-apoptosis, and other aspects. This paper focuses on the protective effects and main mechanisms of SIRT1 on stress-induced vascular senescence and provides clues for preventing and treating diseases related to stress-induced vascular senescence.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251366"},"PeriodicalIF":2.3,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13499030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148796207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and artificial cultivation of a wild strain of <i>Agaricus sinodeliciosus</i>.","authors":"Yanyan Xu, Shasha Feng, Jinping Lan, Longzuo Xin","doi":"10.1515/biol-2025-1365","DOIUrl":"https://doi.org/10.1515/biol-2025-1365","url":null,"abstract":"<p><p>A wild strain collected from the Xinjiang Uygur Autonomous Region underwent morphological observation and molecular biological identification, followed by an investigation into its biological and cultural characteristics. The results identified the strain as <i>Agaricus sinodeliciosus</i>, with an optimal nitrogen source of cow manure at 10 g/L and an optimal carbon-to-nitrogen (C/N) ratio of 30:1. The suitable temperature range for mycelial growth was 21-27 °C, with an optimal pH value of 7. The recommended stock culture medium comprised 53.86 % wheat, 1.74 % lime, 4.4 % cow manure, and 40 % straw fermented material, with a pH of 7.5. The optimal medium for cultivation consisted of 30 % corn straw, 23.5 % corncob, 45 % cow manure, and 1.5 % lime, with a C/N ratio of 30:1, a pH of 7.5, and 65 % moisture content. <i>A. sinodeliciosus</i> can fruit through three cultivation methods: soilless, soil-covered, and frame-based cultivation. This study explores and optimizes the biological characteristics and cultivation conditions of <i>A. sinodeliciosus</i>, providing a reference for its large-scale production.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251365"},"PeriodicalIF":2.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13478923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148796179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of artichoke extracts and probiotic on maternal immunological activation, valproic acid-induced oxidative stress, and gut leakiness in a rat model of autism.","authors":"Maha K Alaskar, Mona Alonazi, Abir Ben Bacha, Abdulaziz Mohammad Alamri, Musarat Amina, Nawal M Al Musayeib, Sameera Abuaish, Afaf K El-Ansary","doi":"10.1515/biol-2025-1355","DOIUrl":"10.1515/biol-2025-1355","url":null,"abstract":"<p><p>Maternal health during pregnancy is a leading factor influencing offspring risk. This study investigated whether postnatal dietary supplementation could reduce oxidative stress and gut leakiness in a rat model of autism spectrum disorder (ASD). Male rat pups were prenatally exposed to valproic acid (VPA) or lipopolysaccharide (LPS) to induce ASD-like conditions. A total of 54 offspring of Wistar albino rats were divided into nine groups to evaluate various postnatal treatments, including an artichoke-based prebiotic (AR), probiotics (Pro), and omega-3 fatty acids (ω3). The experimental design also included control groups (saline, VPA-only, and LPS-only), as well as a protective regimen in which AR was administered both prenatally and postnatally. Oxidative stress and gut permeability \"leakiness\" were assessed using Enzyme-Linked Immunosorbent Assay (ELISA). Prenatal exposure to VPA and LPS was associated with increased oxidative stress levels in brain homogenates, accompanied by a significant decrease in glutathione (GSH). Additionally, elevated plasma levels of gut permeability biomarkers were observed. In the VPA model, treatment with artichoke-derived prebiotics - administered either prenatally, postnatally, or in combination with probiotics - effectively improved oxidative stress markers, as evidenced by a significant increase in GSH levels. Conversely, similar interventions in the LPS-induced maternal immune activation model did not significantly ameliorate oxidative stress, although a modest increase in GSH levels was noted. Plasma levels of gut permeability biomarkers did not show significant improvement in either model following treatment with artichoke-derived probiotics alone or in combination with probiotics and omega-3 fatty acids. However, intestinal fatty acid-binding protein levels were significantly reduced in all treatment groups across both models. In contrast, lipopolysaccharide-binding protein (LBP) levels were not significantly reduced by artichoke extract monotherapy in either model, although combination therapy with probiotics and/or omega-3s led to significant reductions in LBP. These results support the use of both VPA and LPS as complementary models for studying ASD. The VPA model, characterized by direct and predictable neurotoxic effects, appears to be more suitable for evaluating preventive interventions. In contrast, the LPS model more accurately captures the complex immune-inflammatory mechanisms implicated in ASD, highlighting the need for broader and more individualized treatment strategies. The differing responses to artichoke-based interventions in these models underscore the importance of considering ASD etiology when designing dietary and microbiome-targeted therapies.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251355"},"PeriodicalIF":2.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13478924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148796204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual inhibitory potential of N-methylcytisine against GSK-3β and AChE: implications for Alzheimer's disease treatment.","authors":"Shirin Tarbiat, Nigar Kantarci-Carsibasi","doi":"10.1515/biol-2025-1363","DOIUrl":"10.1515/biol-2025-1363","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by memory loss and irreversible cognitive decline. Glycogen synthase kinase 3β (GSK-3β) is significant in tau hyperphosphorylation and neurodegeneration. The cholinergic hypothesis of AD links cognitive impairment to reduced synaptic acetylcholine (ACh). Increased acetylcholinesterase (AChE) activity exacerbates this issue. To search for a potential dual GSK-3β/AChE inhibitor, we focused on N-methylcytisine. This natural cytisine-derived alkaloid has not been studied for its various biological effects on the prevention of AD. The <i>in vitro</i> results indicated that N-methylcytisine displayed promising activity against GSK-3β and AChE with IC₅₀ values of 11 and 22.7 µM, respectively. GSK-3β kinetic study according to the varying substrate or ATP concentrations at different N-methylcytisine levels revealed mixed-type inhibition. The results of the integrated in silico workflow indicate that N-methylcytisine exhibited favorable binding to AChE (docking score -9.6 kcal/mol; MM-GBSA -70.2 kcal/mol), comparable to the reference inhibitor galantamine (-10.8 kcal/mol; -70.6 kcal/mol). In contrast, its interaction with GSK-3β was more moderate (-5.3 kcal/mol; -50.3 kcal/mol) relative to staurosporine (-8.5 kcal/mol; -82.6 kcal/mol), consistent with its smaller scaffold. Overall, the results support a flexible, mixed-type interaction profile and highlight N-methylcytisine as a promising dual-acting candidate for Alzheimer's disease.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251363"},"PeriodicalIF":2.3,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13474028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148765863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Darutoside ameliorates adjuvant-induced arthritis in mice by repolarizing M1/M2 macrophages through inhibition of JAK2-STAT3-p65 signaling pathway.","authors":"Jun Wang, Shiya Gao, Mingsong Meng, Zilin Zhang, Sen Zhang, Jing Han, Dongsheng Wang","doi":"10.1515/biol-2025-1357","DOIUrl":"10.1515/biol-2025-1357","url":null,"abstract":"<p><p>Darutoside (DA), a diterpenoid compound derived from <i>Herba Siegesbeckiae</i>, possesses notable anti-inflammatory properties. This study evaluated the effectiveness of DA in treating rheumatoid arthritis (RA) symptoms and elucidated the relevant mechanisms. The anti-RA efficacy of DA was investigated in a Complete Freund's Adjuvant (CFA)-induced arthritis mouse model, while RAW264.7 macrophages were used to examine DA's impact on macrophage polarization <i>in vitro</i>. Targets of DA for RA were predicted through protein-protein interaction networks and pathway enrichment analysis. Subsequently, mechanisms were verified through <i>in vitro</i> assays. The results showed that DA alleviated the symptoms in the CFA mouse model, as evidenced by reduced clinical scores, attenuated paw swelling, and decreased inflammatory response. Moreover, DA induced repolarization of M1/M2 macrophages in both the CFA model mice and RAW264.7 cells. Network pharmacology analysis suggested that DA may exert its anti-RA effects by modulating the JAK-STAT signaling pathway. Western blotting confirmed that DA inhibited the JAK2-STAT3-p65 pathway, thereby suppressing M1 macrophage polarization. Furthermore, the repolarization effect of DA on macrophages was counteracted in JAK2-silenced cells. These findings indicate that DA attenuates arthritis symptoms in CFA mice, and this effect may be attributed to its regulation of macrophage M1/M2 polarization through suppression of the JAK2-STAT3 pathway and downstream p65 activation.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251357"},"PeriodicalIF":2.3,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13449442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in plasma inflammatory injury factors and their clinical significance in pediatric bacterial pneumonia and sepsis.","authors":"Xia An, Haihua Chen, Zijun Ye","doi":"10.1515/biol-2025-1344","DOIUrl":"10.1515/biol-2025-1344","url":null,"abstract":"<p><p>Bacterial pneumonia and sepsis are common severe infectious diseases in pediatrics. The overlapping early clinical manifestations of these two conditions often lead to misdiagnosis. Establishing a precise early differential diagnostic method is crucial for improving patient outcomes. This retrospective cohort study enrolled 126 children with bacterial pneumonia, 116 children with sepsis, and 65 healthy children as controls. Plasma procalcitonin (PCT), C-reactive protein (CRP), and peripheral blood leukocyte subset indicators were measured, and their differential diagnostic efficacy was analyzed. The results showed that PCT, CRP, white blood cell count, and absolute neutrophil count (NE#) were significantly higher in the sepsis group than in the bacterial pneumonia group (<i>P</i> < 0.05). The area under the curve for PCT in differentiating sepsis was 0.85, with an optimal cut-off value of 5.8 ng/mL. The optimal cut-off values for CRP in diagnosing sepsis and bacterial pneumonia were 28.5 mg/L and 20.0 mg/L, respectively, which could help distinguish local from systemic infection. The diagnostic value of NE# was significantly superior to that of the neutrophil percentage (AUC: 0.79 vs. 0.65). The sepsis group also had a longer disease course and a higher proportion of patients with a history of previous hospitalization (<i>P</i> < 0.05). The study confirmed that PCT and CRP have significant differential diagnostic value for pediatric bacterial pneumonia and sepsis, with PCT showing superior efficacy for identifying sepsis. The differentiated CRP cut-off values could reflect infection severity, and NE# was more suitable as an inflammatory assessment indicator in pediatric infections. Combining these biomarkers with clinical features like disease course and prior hospitalization history can guide early and precise clinical differentiation, providing a reference for timely targeted anti-infective therapy.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251344"},"PeriodicalIF":2.3,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13422573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}