Open Life Sciences最新文献

{"title":"Rapid detection of the <i>GJB2</i> c.235delC mutation based on CRISPR-Cas13a combined with lateral flow dipstick.","authors":"Yueqin Deng, Juan Xu, Ming Yang, Yin Huang, Yifang Yang","doi":"10.1515/biol-2025-1064","DOIUrl":"https://doi.org/10.1515/biol-2025-1064","url":null,"abstract":"<p><p>Hereditary hearing loss, an auditory neuropathy disorder, is characterized by its high prevalence and significant impact on the quality of life of those affected. In Chinese populations, the most prevalent gap junction beta-2 (<i>GJB2</i>) mutation hotspot is c.235delC. Currently available genetic tests require expensive instruments and specialized technicians or have long testing cycles and high costs, and therefore cannot meet point-of-care testing (POCT) requirements. The objective of this study was to evaluate the viability of a POCT kit. In only 42 min, we successfully identified the <i>GJB2</i> mutation site c.235delC by integrating CRISPR-Cas nucleic acid detection with recombinase-aided amplification (RAA) and a lateral flow dipstick (LFD) method. This method has the capacity to detect low-abundance nucleic acids (as low as 10² copies/μL) and low mutation frequency (20%), in addition to accurately distinguishing wild-type, homozygous, and heterozygous mutation. This approach was utilized to assess blood samples from a total of 31 deaf patients and 5 healthy volunteers. All results were subsequently confirmed through the implementation of Sanger sequencing. Our detection results were consistent with Sanger sequencing results. The diagnostic sensitivity and specificity were 100%. The combination of CRISPR-Cas13a and LFD may be a promising method for POCT of deafness genes.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251064"},"PeriodicalIF":1.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABI3BP can inhibit the proliferation, invasion, and epithelial-mesenchymal transition of non-small-cell lung cancer cells.","authors":"Jian Wu, Xiaokun Yan, Zewen Cheng","doi":"10.1515/biol-2022-1034","DOIUrl":"https://doi.org/10.1515/biol-2022-1034","url":null,"abstract":"<p><p>Lung cancer, especially non-small-cell lung cancer (NSCLC), has a poor 5-year survival rate below 20%, with factors like smoking, air pollution, and genetic mutations contributing to its development. ABI3BP, an extracellular matrix protein, inhibits NSCLC progression by regulating key signaling pathways; however, its exact mechanisms remain elusive. This study aimed to explore ABI3BP's role in NSCLC and its impact on these pathways. We found that ABI3BP expression was significantly reduced in NSCLC cells compared to normal controls. Overexpression of ABI3BP in NSCLC cells resulted in a substantial reduction in cell growth and motility and induced cell cycle arrest. Furthermore, its overexpression suppressed the epithelial-mesenchymal transition (EMT) process in NSCLC cells. In addition, ABI3BP overexpression inhibited the MAPK/ERK pathway in NSCLC cells. Collectively, ABI3BP functions as a tumor suppressor in NSCLC by targeting the MAPK/ERK axis, thereby regulating cell proliferation, motility, and EMT. These findings suggest that ABI3BP represents a potential therapeutic target for NSCLC treatment.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221034"},"PeriodicalIF":1.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in blood glucose and metabolism in hyperuricemia mice.","authors":"Fan Yang, Yan Zhao, Xingsan Li, Fengge Lou","doi":"10.1515/biol-2022-1057","DOIUrl":"https://doi.org/10.1515/biol-2022-1057","url":null,"abstract":"<p><p>The aim of this study is to explore the changes in blood uric acid level, blood glucose, and metabolism in hyperuricemia (HUA) mice. The urate oxidase gene of C57BL/6J mouse is knocked out by targeted gene modification technology, and a spontaneous HUA mouse model is established. In the experiment, 20 urate oxidase gene knockout homozygous and wild type C57BL/6J mice are selected to construct the experimental group (EG) and the control group (CG), and the mice in both groups receive multiple intraperitoneal injections of low dose streptozotocin (STZ) solution. The changes in metabolic related indicators such as blood glucose, pancreatic β cell function, water intake, urination, food intake, and body weight are observed and compared between the EG and CG mice. Baseline indicators other than body weight between the two groups of mice before the experiment have no significant difference, <i>P</i> > 0.05. After the injection of STZ solution, body weight between the two groups has significant difference, <i>P</i> < 0.05. Before the experiment and less than 19 days after the start of the experiment, daily water intake and urine output between the two groups of mice have no significant difference, <i>P</i> > 0.05. After the experiment reaches 19 days, two groups' water intake and urine output have significant difference, <i>P</i> < 0.05. Daily food intake between the two groups of mice has no significant difference, <i>P</i> > 0.05. Before the injection of STZ solution, fasting blood glucose levels between the two groups of mice has no significant difference, <i>P</i> > 0.05. The plasma insulin level of the EG mice was higher than that of the CG mice, with significant difference (<i>P</i> < 0.05). At the same time, the Homeostasis Model Assessment of Insulin Resistance index and fasting blood uric acid level of the EG mice were overall higher than those of the CG mice, with significant difference (<i>P</i> < 0.05). From the seventh day after the injection of STZ solution, the random blood glucose level, fasting blood glucose level, fasting insulin level, and blood uric acid level of the EG mice were higher than those of the CG mice, with significant difference (<i>P</i> < 0.05). For spontaneous HUA mice, the continuous increase in blood uric acid level caused by the disease may cause the increase in blood sugar content, thus promoting the occurrence of diabetes. Second, the content of uric acid in spontaneous HUA mice is maintained at a high level, which will bring or aggravate the damage of pancreatic islet β cells.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221057"},"PeriodicalIF":1.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IL-11 promotes Ang II-induced autophagy inhibition and mitochondrial dysfunction in atrial fibroblasts.","authors":"Jun Wang, Qianyu Zhang, Yunjie Han, Jun Zhang, Nan Zheng","doi":"10.1515/biol-2025-1063","DOIUrl":"https://doi.org/10.1515/biol-2025-1063","url":null,"abstract":"<p><p>This study aimed to investigate potential targets for the pathogenesis of atrial fibrillation to facilitate the development of effective treatments. Atrial fibroblasts were isolated and stimulated with 1 μM angiotensin-II (Ang-II) for 24 h. To increase interleukin 11 (IL-11) expression, overexpression plasmids were transfected into atrial fibroblasts. The role and the underlying mechanism of IL-11 in atrial fibrillation were examined by immunofluorescence, measurements of reactive oxygen species (ROS) and mitochondrial membrane potential (MMP), and western blotting assays. Results demonstrated that IL-11 was upregulated in Ang-II-elicited atrial fibroblasts. Ang-II treatment increases alpha-smooth muscle actin (α-SMA), ROS and MMP levels, and p62 expression but decreases microtubule-associated protein light chain 3 II/I (LC3 II/I) and Beclin-1 expressions in atrial fibroblasts. These effects were further amplified by IL-11 overexpression. Mechanistically, the mammalian target of rapamycin (mTOR) pathway expression was enhanced in Ang-II-induced atrial fibroblasts, which was further elevated by IL-11 upregulation. IL-11 facilitates Ang II-induced differentiation of atrial fibroblasts into myofibroblasts by promoting oxidative stress, mitochondrial dysfunction, and autophagy inhibition through the mTOR pathway.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251063"},"PeriodicalIF":1.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium-sensing receptors promoted Homer1 expression and osteogenic differentiation in bone marrow mesenchymal stem cells.","authors":"Kainan Liu, Tianjie Xu, Jiaxin Fan, Yueyuan Li, Xiaoling Guo, Hui Zhang, Qian Wang","doi":"10.1515/biol-2022-1059","DOIUrl":"10.1515/biol-2022-1059","url":null,"abstract":"<p><p>Homer1 interacts with calcium-sensing receptors (CaSRs) in osteoblasts (OBs), with both CaSR and Homer1 playing essential roles in AKT phosphorylation. This study investigated the impact of CaSR on Homer1 expression during the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) at morphological, imaging, and molecular levels, both <i>in vivo</i> and <i>in vitro</i>. A post-oophorectomy osteoporosis model was established in Sprague-Dawley rats, validated through micro-computed tomography, hematoxylin-eosin staining, and biomechanical testing to assess <i>in vivo</i> changes in CaSR expression. BMSCs were isolated from 3 week-old SD rats for <i>in vitro</i> OB differentiation studies, wherein osteogenic differentiation was induced alongside changes in CaSR expression. Morphological alterations were examined using transmission electron microscopy and immunofluorescence staining. Furthermore, the protein and mRNA levels of OB-specific genes were quantified by Western blot and reverse transcription quantitative real-time polymerase chain reaction, with Homer1-related proteins also assessed. Results showed a reduction in CaSR and Homer1 expression in the ovariectomized group. In cellular studies, CaSR activation upregulated AKT, Homer1, and osteogenic markers, promoting cell differentiation. In conclusion, CaSR enhances OB differentiation, likely via Homer1-mediated regulation of AKT signaling, suggesting CaSR as a potential therapeutic target for osteoporosis.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221059"},"PeriodicalIF":1.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review on role of honey in disease prevention and treatment through modulation of biological activities.","authors":"Arshad Husain Rahmani, Ali Yousif Babiker","doi":"10.1515/biol-2025-1069","DOIUrl":"10.1515/biol-2025-1069","url":null,"abstract":"<p><p>Honey has been used for centuries due to its health-promoting properties. Honey and its bioactive compounds regulate oxidative stress, inflammation, and other biological activities, making it a promising natural remedy. Its role as anti-diabetic, wound healing, cardioprotective, anti-microbial, and hepatoprotective potential has been proven through <i>in vitro</i> and <i>in vivo</i> studies. Moreover, its role in disease management has been reported through the inhibition of pro-inflammatory enzymes and downregulation of pro-inflammatory cytokine expression and secretion. Besides, it exerts modulatory actions on various signaling pathways such as nuclear factor-κB, tumor suppressor genes, apoptosis, angiogenesis, and MAPK pathway. The main aim of this review is to present a wide-ranging overview of the current evidence regarding the impact of honey on the management of various pathogenic conditions. However, further research based on animal models and clinical trials is required to fully understand the mechanisms of action and safety in the management of various diseases. There is limited clinical data on honey and its mechanisms of action. However, comprehensive clinical studies are needed to fully investigate the potential health benefits of honey, including its efficacies, safety, bioavailability, and underlying mechanisms of action.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251069"},"PeriodicalIF":1.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Widely targeted metabolomics of different tissues in <i>Rubus corchorifolius</i>.","authors":"Xiangmei Chen, Liang Liang, Shan Chang, Xiang Chun, Yun Qing","doi":"10.1515/biol-2022-0996","DOIUrl":"10.1515/biol-2022-0996","url":null,"abstract":"<p><p><i>Rubus corchorifolius</i>, a medicinal plant of the <i>Rosaceae</i> family, is known for its diverse bioactive compounds. This study employs widely targeted metabolomics to investigate the metabolic profiles of leaf, stem, and flower tissue from <i>R. corchorifolius</i>. Using ultra-performance liquid chromatography coupled with tandem mass spectrometry, we identified 1,946 metabolites across the three tissue types. Multivariate statistical analyses revealed distinct metabolic signatures for each tissue, with flowers showing the most distinctive profile. Differential accumulation of flavonoids, phenolic acids, and primary metabolites reflected the specialised functions of each tissue type. Pathway enrichment analysis highlighted tissue-specific metabolic activities, including flavonoid biosynthesis in flowers and chlorophyll metabolism in leaves. This comprehensive metabolic characterisation provides a foundation for further investigations into the biosynthetic pathways and physiological functions of bioactive compounds in <i>R. corchorifolius</i>, potentially guiding future applications in medicine and agriculture.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20220996"},"PeriodicalIF":1.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-temperature requirement serine protease A2 inhibitor UCF-101 ameliorates damaged neurons in traumatic brain-injured rats by the AMPK/NF-κB pathway.","authors":"Tian-Wen Qiu, Zhan Jin, Zhi-Zhan Fu, Xin-Jiang Yan, Cheng-Peng Zhan, Hui-Wen Zheng, Mei-Ying Li, Guo-Feng Yu","doi":"10.1515/biol-2022-0971","DOIUrl":"10.1515/biol-2022-0971","url":null,"abstract":"<p><p>Traumatic brain injury (TBI) leads to permanent damage, including neurological deficits, cognitive deficits, and cerebral edema. The specific inhibitor of serine protease Omi/high-temperature requirement A2 (Omi/HtrA2), UCF-101, exerts neuroprotective effects, but its role in TBI remains unclear. Eighty-four male Sprague Dawley rats were randomized to control, TBI, UCF-101 of low dose (1.5 μmol/kg), middle dose (3.0 μmol/kg), and high dose (6.0 μmol/kg), Compound C (AMPK inhibitor, 20 mg/kg), and high dose + Compound C groups. TBI rat modeling was operated by the controlled cortical impact method. Modified neurological severity score (mNSS) cognitive function, cerebral edema index, hematoxylin-eosin staining, TUNEL staining for apoptosis, ethidium bromide staining for blood-brain barrier (BBB) permeability, enzyme-linked immunosorbent assay for inflammation response, and Western blot analysis were performed. In TBI rats, UCF-101 caused decreased mNSS score, brain edema, neuronal apoptosis, as well as P-NF-κBp65/NF-κBp65, tumor necrosis factor-α, interleukin (IL)-1β, and IL-8 expression, while P-AMPK/AMPK, zonula occludens protein, Occludin, and Claudin-5 expression increased, accompanied with up-regulated cognitive function. Moreover, Compound C further exacerbated brain tissue lesions, neuronal damage, inflammation response, and neuronal apoptosis, while high-dose UCF-101 offset its effect. UCF-101 may inhibit apoptosis and BBB permeability to exert neuroprotective effects in TBI rats by regulating the AMPK/NF-κB pathway, advancing UCF-101 clinical applications for TBI treatment.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20220971"},"PeriodicalIF":1.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of Aurora kinase B alleviates high glucose-triggered trophoblast cells damage and inflammation during gestational diabetes.","authors":"Yuzhuo Ma, Yongyun Shi, Yujie Liu","doi":"10.1515/biol-2022-1031","DOIUrl":"10.1515/biol-2022-1031","url":null,"abstract":"<p><p>This research investigates how Aurora kinase B (AURKB) functions in trophoblast cells when they are exposed to high levels of glucose during gestational diabetes. The findings from RT-qPCR and western blotting show that when in a high-glucose environment, AURKB expression increases in both the placenta and trophoblast cells of patients with gestational diabetes mellitus. Additionally, when AURKB is silenced in high-glucose conditions, it leads to boosted proliferation of trophoblast cells and reduced inflammation. Knockdown of AURKB inhibits the expression of phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway in high glucose (HG) environment. Knockdown of AURKB may ameliorate injury and inflammatory responses in HG-exposed trophoblast cell lines in part by regulating the PI3K/AKT signaling pathway.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221031"},"PeriodicalIF":1.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and comparative study of <i>Bacillus</i> consortia for cellulolytic potential and cellulase enzyme activity.","authors":"Ogechukwu Bose Chukwuma, Mohd Rafatullah, Riti Thapar Kapoor, Husnul Azan Tajarudin, Norli Ismail, Mahboob Alam, Masoom Raza Siddiqui","doi":"10.1515/biol-2025-1066","DOIUrl":"10.1515/biol-2025-1066","url":null,"abstract":"<p><p>Lignocellulosic biomass, owing to its recalcitrant nature, requires a consortium of enzymes for its breakdown. The present study deals with the isolation of cellulolytic bacterial strains from landfill leachate collected from the Pulau Burung landfill site of Penang, Malaysia, and consortia were constructed to test their cellulolytic efficiency. The dinitro salicylate method was used for the estimation of enzyme activity, and consortia were compared with promising bacterial strains. The combined potential of promising bacterial strains was optimized at varying experimental conditions to detect their maximum cellulolytic activity. The results showed that eight bacterial strains reflected hydrolytic activities, and these were identified by 16S rDNA sequence as <i>Bacillus subtilis, Bacillus pumilus, Bacillus proteolyticus, Bacillus paramycoides, Bacillus cereus, Bacillus altitudinis, Bacillus niacin,</i> and <i>Bacillus thuringiensis.</i> Consortia A included <i>Bacillus proteolyticus, Bacillus subtilis, Bacillus pumilus,</i> and <i>Bacillus paramycoides</i> and reflected high thermophilic inclination as the optimal temperature was 45°C at pH 6 with the highest cellulase activity of 0.90 U/ml. Consortia B included <i>Bacillus cereus, Bacillus altitudinis, Bacillus niacin,</i> and <i>Bacillus thuringiensis</i> and showed a cellulase activity of 0.78 U/ml at 38°C and pH 6. The results reflected the significant potential of these <i>Bacillus</i> strains and consortia in the breakdown of cellulose into useful end products. The consortia further proved that a synergistic relationship was more favourable for bioconversion processes.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251066"},"PeriodicalIF":1.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}