Open Life Sciences最新文献

{"title":"Effect of sex ratio on the life history traits of an important invasive species, <i>Spodoptera frugiperda</i>.","authors":"Su-Ran Wu, Hui Wang, Chun-Jie Zhao, Yan Xiong, Jun-Hua Ren","doi":"10.1515/biol-2022-0873","DOIUrl":"https://doi.org/10.1515/biol-2022-0873","url":null,"abstract":"<p><p>The fall armyworm (FAW), <i>Spodoptera frugiperda</i>, is a dangerous migratory pest. Evaluating the effect of sex ratio on the FAW offspring population is particularly important for field control. In this study, five different sex ratio treatments (female/male = 3:1, 2:1, 1:1, 1:2, and 1:3) were conducted to investigate the effects of sex ratio on the life history traits of FAW. The results showed that sex ratio significantly affected lifetime fecundity, developmental duration of the preadult stage, hatch rate, and emergence rate but had no effect on longevity of parental and offspring adults, larval duration, pupation rate, or number of eggs/moth of offspring. The lifetime fecundity and hatch rate of parental adults and the number of adult offspring/moth were the lowest when the sex ratio was 3:1, while the lifetime fecundity and number of adult offspring/moth were the highest and pupation duration was the shortest when the sex ratio was 1:1. The number of eggs/moth of parental adults and total adults in the F1 generation were higher in male-biased groups than in female-biased groups, and male annihilation appears to be a more effective control strategy. These findings have implications for improving laboratory rearing, population forecasting, and control of FAW in the field.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20220873"},"PeriodicalIF":1.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical analysis of <i>Stachys iva</i>: Discovering the optimal extract conditions and its bioactive compounds.","authors":"Aikaterina Vantsioti, Ioannis Makrygiannis, Vassilis Athanasiadis, Stavros I Lalas, Paraskevi Mitlianga","doi":"10.1515/biol-2022-1053","DOIUrl":"10.1515/biol-2022-1053","url":null,"abstract":"<p><p>The Lamiaceae family is one of the widest plant families among Greek flora, consisting of a great variety of species, with the genus <i>Stachys</i> being one of its largest representatives, spread to most continents. The genus <i>Stachys</i> is also known for its beneficial properties and has been used for years as a traditional remedy for healing various health conditions. <i>Stachys iva</i>, an endemic plant in the Kozani Regional unit, has also been consumed as an infusion by locals and is reported to relieve common cold symptoms, have antimicrobial properties, and contribute to normalizing blood glucose levels. The present study aimed to identify the chemical compounds (such as phenolic acids, flavonoids, and phenylethanoid glycosides) responsible for the herb's pharmacological properties and determine the optimal extraction conditions to gather an extract with high therapeutic value without solvent and energy waste. Experiments conducted proved that extracting by simple stirring with deionized water for 75 min at 80°C is the best option. In contrast, the extract's total polyphenol content was determined, and the compounds were identified by high-performance liquid chromatography analysis. In addition, other methods were utilized (e.g., ferric-reducing antioxidant power assay and 2,2-diphenyl-1-picrylhydrazyl antiradical activity assay) to reveal potent antioxidant, anti-hydrogen peroxide, and anti-inflammatory activity, while the correlation between these properties and extraction conditions was also examined.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221053"},"PeriodicalIF":1.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary analysis of the role of small hepatitis B surface proteins mutations in the pathogenesis of occult hepatitis B infection via the endoplasmic reticulum stress-induced UPR-ERAD pathway.","authors":"Chengrong Huang, Hao Zhang, Jing Wang, Jianfei Li, Qian Liu, Qiyin Zong, Yunyun Zhang, Qin Wang, Qiang Zhou","doi":"10.1515/biol-2022-0951","DOIUrl":"10.1515/biol-2022-0951","url":null,"abstract":"<p><p>A growing body of evidence has shown that hepatitis B surface antigen (HBsAg) mutations can influence the occurrence of occult hepatitis B infection (OBI), particularly amino acid substitutions in small hepatitis B surface proteins (SHBs). The mechanistic basis for these results, however, remains unclear. This study was designed to explore the potential impact and mechanisms of OBI-related SHBs mutations on serum HBsAg. Huh7 and HepG2 cells were transfected with plasmids encoding wild-type (WT) or OBI-related SHB mutation-containing sequences, after which a chemiluminescence approach was used to detect HBsAg levels in cell culture supernatants. Western blotting was further used to assess HBsAg and endoplasmic reticulum stress (ERS)-related protein levels in lysates prepared from these cells, while the localization of HBsAg within cells was assessed via immunofluorescent staining. Cells transfected with OBI-related SHB mutation-encoding plasmids exhibited lower supernatant HBsAg levels than cells transfected with WT plasmids. Intracellular and extracellular HBsAg levels in these mutant plasmid-transfected cells were lower relative to those for WT plasmid-transfected cells, and HBsAg accumulation within the ER was detected via immunofluorescent staining in cells transfected with OBI-related SHB mutation-encoding plasmids, ERS-related protein content was also significantly increased in mutant plasmid-transfected cells as compared to those in the WT group. These results suggest that proteins harboring OBI-related mutations may tend to accumulate in the ER, thereby triggering an ERS response and impairing the transcription and translation of HBsAg via the activation of the unfolded protein response and ER-associated protein degradation pathway. These effects ultimately reduce the overall assembly of HBV virions in the ER and their associated secretion.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20220951"},"PeriodicalIF":1.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification.","authors":"Gulam Jeelani Dar, Ruqeya Nazir, Shakil A Wani, Saleem Farooq, Tariq Aziz, Thamer H Albekairi","doi":"10.1515/biol-2022-1006","DOIUrl":"10.1515/biol-2022-1006","url":null,"abstract":"<p><p>Genomic DNA (gDNA) extraction is an important step in many molecular studies of fungal biology, and it is necessary to evaluate the efficiency, cost-effectiveness, and efficacy of different extraction methods to ensure successful amplification of the target gene and minimize deoxyribonucleic acid (DNA) degradation. The modified cetyltrimethylammonium bromide (CTAB) method was found to be effective in releasing high molecular weight gDNA with minimal protein contamination. Based on anticipated gDNA yield and quality, extraction time, cost effectiveness, successful amplification, and waste management, our findings serve as a guide for selecting techniques of gDNA extraction from fungal species. This study presents a modified CTAB method for extracting DNA from a variety of fungal species including <i>Aspergillus</i>, <i>Penicillium</i>, <i>Alternaria</i>, <i>Dothiorella,</i> and <i>Fusarium</i>. Comparison of three cell crushing methods reveals similar gDNA yields, demonstrating the method's effectiveness. Furthermore, the modified CTAB method is cost-effective and safe, eliminating the need for grinding with liquid nitrogen or bead beating. The method has a potential use for nucleic-based fungal disease diagnosis such as fish fungal diseases, plant pathogens, fruit rot associated pathogens, and human fungal diseases as we were successful in PCR amplifying several gene loci from varied fungal pathogens.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221006"},"PeriodicalIF":1.7,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteome differences of dental stem cells between permanent and deciduous teeth by data-independent acquisition proteomics.","authors":"Suping Zhang, Yuqing Liu, Jin Dong, Min Jiao, Yongchun Gu, Liling Chen, Na Yuan, Jianrong Wang, Dezhao Yang, Fanwen Meng","doi":"10.1515/biol-2022-0998","DOIUrl":"10.1515/biol-2022-0998","url":null,"abstract":"<p><p>Dental pulp stem cells hold significant prospects for tooth regeneration and repair. However, a comprehensive understanding of the molecular differences between dental pulp stem cells (DPSC, from permanent teeth) and stem cells from human exfoliated deciduous teeth (SHED, from deciduous teeth) remains elusive, which is crucial for optimizing their therapeutic potential. To address this gap, we employed a novel data-independent acquisition (DIA) proteomics approach to compare the protein expression profiles of DPSC and SHED. Based on nano-LC-MS/MS DIA proteomics, we identified over 7,000 proteins in both cell types. By comparing their expression levels, 209 differentially expressed proteins were identified. Subsequent Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses, along with protein-protein interaction network construction, revealed significant metabolic differences and key regulatory nodes. DPSC exhibited significantly higher expression of proteins belonging to the NDUFB family, SMARC family, RPTOR and TLR3. These proteins are known to be involved in critical cellular processes such as mitochondrial energy metabolism, mTOR-related autophagy pathway, and innate immune response. Conversely, SHED displayed elevated expression of AKR1B family, which participated in glycerolipid metabolism and adipogenic differentiation, PRKG1, MGLL and UQCRB proteins associated with thermogenesis. These findings highlight the specific proteomic landscape of DPSC and SHED, suggesting their distinct biological roles and potential applications.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20220998"},"PeriodicalIF":1.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of inflammatory biomarkers for the diagnosis of neonatal sepsis: IL-6, IL-8, SAA, CRP, and PCT.","authors":"Ying Chen, Aixia Yan, Li Zhang, Xiaoming Hu, Liang Chen, Jun Cui, Zichuan Fan, Ying Li","doi":"10.1515/biol-2022-1005","DOIUrl":"10.1515/biol-2022-1005","url":null,"abstract":"<p><p>Neonatal sepsis (NS) is highly likely to cause death; however, early diagnosis of NS is still a great challenge. This study aimed to determine the diagnostic values of IL-6, IL-8, and serum amyloid A (SAA) in NS patients. C-Reactive protein (CRP), procalcitonin (PCT), interleukin (IL)-6, IL-8, and SAA were detected in 120 infants with NS (60 premature infants [NS-PIs] and 60 term infants [NS-TIs]). Sixty noninfected premature infants and 60 noninfected term infants composed the control group. Receiver operating characteristic (ROC) curves were used to determine the sensitivity and specificity of IL-6, IL-8, and SAA alone and in combination with CRP and PCT. The area under the curve (AUC) was calculated to evaluate the diagnostic value. The clinical characteristics of the subjects were recorded. The expression of CRP, PCT, IL-6, IL-8, and SAA was upregulated in patients with NS compared with control subjects. When the SAA cut-off value was 10.18 mg/L, the greatest AUC for the diagnosis of NS-PIs was for SAA (AUC = 0.833, 95% CI 0.762-0.905, <i>P</i> < 0.001). When the CRP cut-off value was 9.562 mg/L, the smallest AUC for the diagnosis of NS-PIs was for CRP (AUC = 0.776, 95% CI 0.684-0.867, <i>P</i> < 0.001). When the IL-8 cut-off value was 52.03 pg/mL, the greatest AUC for the diagnosis of NS-TIs was for IL-8 (0.821). When the IL-8 cut-off value was 52.03 pg/mL, the greatest AUC for the diagnosis of NS-TIs was for IL-8 (AUC = 0.821, 95% CI 0.745-0.898, <i>P</i> < 0.001). When the CRP cut-off value was 13.18 mg/L, the smallest AUC for the diagnosis of NS-TIs was for CRP (AUC = 0.762, 95% CI 0.667-0.857; <i>P</i> < 0.001). Additionally, according to the AUC value, the best combination was SAA and PCT for NS-PI diagnosis, and the best combination was PCT and IL-6 for NS-TI. In conclusion, compared with PCT and CRP, IL-6, IL-8, and SAA are better diagnostic biomarkers. Moreover, PCT combined with SAA is more suitable for diagnosing NS-PIs, and PCT combined with IL-6 is more suitable for diagnosing NS-TIs.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221005"},"PeriodicalIF":1.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-pandemic insights on COVID-19 and premature ovarian insufficiency.","authors":"Yaguang Han, Yang Dai, Kexin Wang, Xin Zhang, Zishen Shao, Xiaolin Zhu","doi":"10.1515/biol-2022-1028","DOIUrl":"10.1515/biol-2022-1028","url":null,"abstract":"<p><p>The COVID-19 pandemic has raised concerns regarding its potential impact on premature ovarian insufficiency (POI). This overview examines the possible interactions between COVID-19 and POI, while also suggesting preventive measures. The viral infection's inflammatory response and immune dysregulation may adversely affect ovarian tissues, leading to inflammation and damage. Additionally, alterations in vascular function could impair ovarian blood flow and hormonal imbalances may disrupt normal ovarian function. Long-term health effects, such as \"long COVID,\" may exacerbate these issues through chronic inflammation and immune dysfunction. Public health measures, such as vaccination and home isolation, may indirectly protect ovarian health by reducing systemic inflammation. Vaccines could mitigate the severity of COVID-19's impact on ovarian function, while isolation may reduce stress and inflammation. However, further research is needed to validate these mechanisms.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221028"},"PeriodicalIF":1.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Hairpin in a haystack</i>: <i>In silico</i> identification and characterization of plant-conserved microRNA in Rafflesiaceae.","authors":"Adhityo Wicaksono, Karlia Meitha, Kiew-Lian Wan, Mohd Noor Mat Isa, Arli Aditya Parikesit, Jeanmaire Molina","doi":"10.1515/biol-2022-1033","DOIUrl":"https://doi.org/10.1515/biol-2022-1033","url":null,"abstract":"<p><p>Rafflesiaceae is a family of endangered plants whose members are solely parasitic to the tropical grape vine <i>Tetrastigma</i> (Vitaceae). Currently, the genetics of their crosstalk with the host remains unexplored. In this study, we use homology-based <i>in silico</i> approaches to characterize micro-RNAs (miRNAs) expressed by <i>Sapria himalayana</i> and <i>Rafflesia cantleyi</i> from published omics data. Derived from secondary structures or hairpins, miRNAs are small regulators of gene expression. We found that some plant-conserved miRNA still exists in Rafflesiaceae. Out of 9 highly conserved miRNA families in plants, 7 families (156/157, 159/319, 160, 165/166, 171, 172, 390) were identified with a total of 22 variants across Rafflesiaceae. Some miRNAs were missing endogenous targets and may have evolved to target host miRNA, though this requires experimental verification. Rafflesiaceae miRNA promoters are mostly inducible by ethylene that mediates stress response in the host but could be perceived by the parasites as a signal for growth. This study provides evidence that certain miRNAs with ancient origins in land plants still exist in Rafflesiaceae, though some may have been coopted by parasites to target host genes.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221033"},"PeriodicalIF":1.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maslinic acid improves mitochondrial function and inhibits oxidative stress and autophagy in human gastric smooth muscle cells.","authors":"Xiaoying Zheng, Shuning Zhang, Qiaobin Chen","doi":"10.1515/biol-2022-1036","DOIUrl":"https://doi.org/10.1515/biol-2022-1036","url":null,"abstract":"<p><p>Functional dyspepsia (FD) is a chronic disease that occurs in the gastroduodenal region and significantly impacts human health. Maslinic acid (MA), a pentacyclic triterpene acid, is the primary bioactive ingredient in Chinese medicinal herbs such as hawthorn, which exhibits beneficial impacts on the regulation of various disease progressions. However, the specific functions and associated pathways of MA in FD progression remain unclear and require further investigation. In this work, it was demonstrated that MA enhanced the cell viability of human gastric smooth muscle cells (HGSMCs). In addition, the mitochondrial dysfunctions induced by carbonyl cyanide 3-chlorophenylhydrazone (CCCP) were rescued after MA treatment. Furthermore, autophagy was increased following CCCP treatment, but this phenomenon was counteracted after MA treatment. The oxidative stress, elevated after CCCP treatment, was alleviated following MA addition. Finally, the AMPK/SIRT1 pathway was suppressed after CCCP stimulation but was re-activated after MA treatment. In conclusion, it was uncovered that MA accelerated HGSMC viability and improved mitochondrial function, inhibited autophagy, alleviated oxidative stress, and stimulated the AMPK/SIRT1 pathway. This discovery may offer new insight into the therapeutic effects of MA in FD progression.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221036"},"PeriodicalIF":1.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis of transcriptome, sRNAome, and degradome involved in the drought-response of maize Zhengdan958.","authors":"Shuqiong Yang, Jiafei Liu, Lingling Cao, Jibao Chen, Pengfei Duan","doi":"10.1515/biol-2022-1044","DOIUrl":"https://doi.org/10.1515/biol-2022-1044","url":null,"abstract":"<p><p>Drought is a major abiotic stress in restricting the growth, development, and yield of maize. As a significant epigenetic regulator, small RNA also functions in connecting the transcriptional and post-transcriptional regulatory network. Further to help comprehending the molecular mechanisms underlying drought adaptability and tolerance of maize, an integrated multi-omics analysis of transcriptome, sRNAome, and degradome was performed on the seedling roots of an elite hybrid Zhengdan958 under drought stress. In this study, 2,911 genes, 32 conserved miRNAs, and 12 novel miRNAs showed a significantly differential expression under drought stress. Moreover, 6,340 target genes of 445 miRNAs were validated using degradome sequencing, forming 281 miRNA-mRNA pairs in control (CK) and drought-stressed (DS) library. These target genes were mainly involved in the plant hormone signal transduction and phenylpropanoid biosynthesis pathways. The integrated multi-omics analysis revealed that five DEmiRNA-mRNA pairs displayed negatively correlated expression patterns, which were also verified by qRT-PCR. Tissue-specific expression profile and regulatory network analysis revealed that miR528a/b-<i>Zm00001d021850</i>, miR408a/b-<i>Zm00001d020794</i>, and miR164e-<i>Zm00001d003414</i> might be essential in root-specific drought stress response of maize Zhengdan958 seedlings. These worthwhile will promote the functional characterization of miRNA-mRNA modules response to drought stress, and potentially contribute to drought-resistance breeding of maize.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221044"},"PeriodicalIF":1.7,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}