Open Life Sciences最新文献

Bee venom acupuncture therapy ameliorates ulcerative colitis by reducing inflammatory response, oxidative stress, and suppressing the NF-κB/HIF-1α pathway. 蜂毒针刺疗法通过降低炎症反应、氧化应激和抑制NF-κB/HIF-1α途径改善溃疡性结肠炎。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2026-05-05 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1326

Jing Liang, Xu Ma, Yuqing Ren, Mo Chen, Huan Ma, Jingbo Guo

{"title":"Bee venom acupuncture therapy ameliorates ulcerative colitis by reducing inflammatory response, oxidative stress, and suppressing the NF-κB/HIF-1α pathway.","authors":"Jing Liang, Xu Ma, Yuqing Ren, Mo Chen, Huan Ma, Jingbo Guo","doi":"10.1515/biol-2025-1326","DOIUrl":"https://doi.org/10.1515/biol-2025-1326","url":null,"abstract":"Ulcerative colitis (UC) is a chronic inflammatory bowel disease that poses a significant challenge to public health due to its relapsing nature and complex pathogenesis. This study investigated the protective effects of bee venom acupuncture on UC and explored the underlying mechanisms using a rat model of UC. The findings demonstrated that bee venom acupuncture significantly restored body weight, reduced disease activity index (DAI) scores, and ameliorated colonic tissue injury. Moreover, bee venom therapy decreased serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8), and downregulated their protein expression in colon tissue. Furthermore, assessment of oxidative stress biomarkers revealed that bee venom treatment significantly reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels while elevating superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) levels in the colon. Western blot and immunohistochemistry analyses indicated that bee venom treatment inhibited NF-κB/HIF-1α pathway. In conclusion, bee venom acupuncture ameliorates UC by reducing inflammatory responses, alleviating oxidative stress, and suppressing NF-κB/HIF-1α pathway, suggesting its potential as a therapeutic strategy for UC management.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251326"},"PeriodicalIF":1.7,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13138559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide in sepsis: pathophysiological mechanisms and therapeutic implications. 一氧化氮在败血症中的作用：病理生理机制和治疗意义。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2026-05-04 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1315

Jun Chen, Zhenghang Li, Zhuo Li, Zhaoqun Zhou

{"title":"Nitric oxide in sepsis: pathophysiological mechanisms and therapeutic implications.","authors":"Jun Chen, Zhenghang Li, Zhuo Li, Zhaoqun Zhou","doi":"10.1515/biol-2025-1315","DOIUrl":"https://doi.org/10.1515/biol-2025-1315","url":null,"abstract":"Sepsis is a life-threatening syndrome defined by organ dysfunction arising from a dysregulated host response to infection. In its more severe forms, it may progress to multiple organ failure and is associated with a substantial increase in mortality risk. Nitric oxide (NO), a gaseous signaling mediator, has attracted attention because of its context-dependent roles in both physiological regulation and pathological processes. NO participates in intracellular signaling pathways and modulates protein function through post-translational mechanisms. In patients with sepsis, altered NO production has been closely linked to circulatory shock, vascular hyporeactivity, and multiple organ dysfunction, with particularly significant effects on cardiovascular and renal systems. Despite extensive investigation, the precise role of NO at different stages of sepsis remains incompletely defined. Clarifying the temporal and mechanistic aspects of NO dysregulation may help determine its utility as a biomarker and as a potential therapeutic target. Future clinical strategies should focus on modulating excessive NO activity without compromising its essential physiological functions, thereby improving the translational feasibility and clinical applicability of NO-directed interventions.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251315"},"PeriodicalIF":1.7,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential expression and functional analysis of circular RNA in ovaries of Tibetan sheep with different fecundity. 环状RNA在不同繁殖力藏羊卵巢中的差异表达及功能分析。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2026-05-04 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1256

Yunxin Jin, Wu Sun, Xiayang Jin, Shike Ma

{"title":"Differential expression and functional analysis of circular RNA in ovaries of Tibetan sheep with different fecundity.","authors":"Yunxin Jin, Wu Sun, Xiayang Jin, Shike Ma","doi":"10.1515/biol-2025-1256","DOIUrl":"https://doi.org/10.1515/biol-2025-1256","url":null,"abstract":"This study represents one of the first investigations of circular RNAs (circRNAs) in the ovaries of Tibetan sheep, with a focus on their potential role in reproductive performance. The aim was to identify circRNAs involved in ovarian development and fertility regulation. High-throughput sequencing was applied to analyze ovarian tissue samples from 20 Tibetan sheep, leading to the identification of 82 differentially expressed circRNAs between the single multiparous (SL) and multiple multiparous (ML) groups, with 35 upregulated and 47 downregulated circRNAs. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed significant associations with pathways involved in cell growth, cell cycle progression, energy metabolism, hormone regulation, and cellular transport. Protein-protein interaction (PPI) analysis identified key target genes, including EP300, CUL1, and oar-miR-654-5p. A comprehensive quaternary regulatory network, integrating circRNA, miRNA, mRNA, and related pathways, was constructed. This study provides novel insights into the molecular mechanisms underlying fertility in Tibetan sheep and identifies potential molecular targets for improving reproductive biotechnology. These findings lay the foundation for future molecular breeding programs aimed at enhancing fertility in Tibetan sheep.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251256"},"PeriodicalIF":1.7,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomes from human urine-derived stem cells in porcine urethral scaffold construction. 人尿源干细胞外泌体在猪尿道支架构建中的应用。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2026-05-04 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1320

Kai-Yue Zhang, Hao Zhong, Wei-De Ma, Xiao-Yan Yang, Li-Zhong Han, Zhi-Zhong Liu

{"title":"Exosomes from human urine-derived stem cells in porcine urethral scaffold construction.","authors":"Kai-Yue Zhang, Hao Zhong, Wei-De Ma, Xiao-Yan Yang, Li-Zhong Han, Zhi-Zhong Liu","doi":"10.1515/biol-2025-1320","DOIUrl":"https://doi.org/10.1515/biol-2025-1320","url":null,"abstract":"This study aims to examine the role of exosomes derived from human urine-derived stem cells (hUSCs-Exo) in the in vitro construction of a composite structure comprising hUSCs and a porcine urethral decellularized matrix. hUSCs-derived exosomes were isolated and characterized, and hUSCs were treated with varying exosome concentrations to assess migration using scratch and Transwell assays. Dio-labeled hUSCs were seeded onto porcine urethral decellularized matrices and grouped by exosome concentration (0, 50, 100 μg/mL). Cell proliferation and distribution were examined under a fluorescence inverted microscope on days 1, 3, and 7. On day 7, samples were paraffin-embedded for histological analysis of cell integration. hUSCs with mesenchymal stem cell (MSC) properties were successfully isolated, and exosomes extracted via centrifugation. hUSCs-exosome (Exo) enhanced cell migration but did not significantly affect proliferation. Dio-labeling and H&E staining confirmed hUSC presence and attachment to the urethral matrix, while CD44 immunohistochemistry confirmed the presence and attachment of hUSCs within the scaffold. Exosomes derived from hUSCs did not significantly enhance cell proliferation in the construction of the porcine urethral decellularized matrix-hUSC complex. The specific exosomal cargo responsible for these differential effects on migration versus proliferation was not examined in this study and will be the focus of future investigations.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251320"},"PeriodicalIF":1.7,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaf structure, physiology and transcriptome response of ancient Populus szechuanica cuttings in southwest China under different drought stresses. 不同干旱胁迫下四川白杨扦插叶片结构、生理及转录组响应

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2026-05-04 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1310

Zekun Zhang, Yanyuan Lu

{"title":"Leaf structure, physiology and transcriptome response of ancient Populus szechuanica cuttings in southwest China under different drought stresses.","authors":"Zekun Zhang, Yanyuan Lu","doi":"10.1515/biol-2025-1310","DOIUrl":"https://doi.org/10.1515/biol-2025-1310","url":null,"abstract":"Against the background of global climate change, increasingly severe drought stress exerts a significant impact on plant growth and yield. This study aimed to clarify the leaf anatomical structure, physiology and biochemistry and transcriptome-level metabolic adaptation mechanisms of ancient P. szechuanica to environmental stress. We selected cuttings of ancient P. szechuanica with a diameter of breast-height (DBH) ≥ 1 m and a tree age of 300-500 years as experimental materials. Natural drought stress was applied to investigate the responses of leaf anatomical structure, physiological and biochemical traits, and transcriptome-level metabolic processes of ancient P. szechuanica under drought stress. The results showed the following changes in leaf anatomical structure under drought stress (compared with the control group, the same below): leaf thickness, pith length and palisade tissue thickness decreased by 49.60 %, 20.1 % and 28.68 %, respectively. The thickness of upper and lower epidermis and spongy tissue first increased and then decreased, with final reductions of 53.13 %, 54.26 % and 50.30 %, respectively. Stomatal length and width also decreased, by 15.67 % and 24.26 % respectively. For physiological and biochemical traits, with the prolongation of drought stress, the soluble sugar content decreased significantly by 7.49 %, while the soluble protein content increased significantly by 44 %.At the transcriptome level, significant differentially expressed genes (DEGs) were screened at different drought stages: 3,353 upregulated and 3,161 downregulated DEGs on the day 4 of drought, 5,208 up-regulated and 9,560 down-regulated DEGs on the day 8, and 15,659 up-regulated and 14,870 down-regulated DEGs on the day 12. These DEGs mediated the drought stress response of P. szechuanica via positive up- or down-regulation.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251310"},"PeriodicalIF":1.7,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The protective role of whole grains and legumes against gastric cancer. 全谷物和豆类对胃癌的保护作用。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2026-05-04 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1303

Sarrah Daban, Manar Adawi, Dania Alsayyad, Rana Youssef, Sabika Allehdan, Aya Hamdan, Reema Tayyem

{"title":"The protective role of whole grains and legumes against gastric cancer.","authors":"Sarrah Daban, Manar Adawi, Dania Alsayyad, Rana Youssef, Sabika Allehdan, Aya Hamdan, Reema Tayyem","doi":"10.1515/biol-2025-1303","DOIUrl":"https://doi.org/10.1515/biol-2025-1303","url":null,"abstract":"Despite significant advancement in medicine, survival rates for gastric cancer (GC) remain low, especially when diagnosis occurs at advanced stages. Therefore, prevention through lifestyle and dietary modification has become a critical strategy to reduce the burden of GC. This review explores the relationship between the consumption of whole-grains (WG) and legumes and the risk of GC, and highlights the biological mechanisms that may contribute to their protective effects. A comprehensive search was conducted in PubMed/MEDLINE, Embase, ProQuest, Google Scholar, Web of Science, and Scopus for English-language studies published between 1996 and 2025. The search terms included gastric cancer, whole grains, legumes, beans, lentils, soy beans, and peanuts. Observational studies assessing WG and legumes intake in relation to GC risk were reviewed. Observational studies show controversial findings; the majority indicate that the consumption of WG and legumes is associated with lower risk of GC. WG are rich in fiber, antioxidants, vitamins, minerals, and phytochemicals as compared with refined grains, which help mitigate oxidative stress, inflammation, and DNA damage - key pathways involved in gastric carcinogenesis. Similarly, legumes contain beneficial bioactive compounds such as polyphenols, flavones, and isoflavones that may inhibit tumor initiation and slow progression. Incorporating WG and legumes into daily diets may represent an effective, sustainable, and accessible public health approach to lowering GC risk, while also supporting metabolic, immune, and gut health.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251303"},"PeriodicalIF":1.7,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KIF14-AKT axis regulates ferroptosis sensitivity in triple-negative breast cancer. KIF14-AKT轴调控三阴性乳腺癌铁下垂敏感性。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2026-05-04 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1324

Chunyu Shi, Weiyi Fang, Yingqi Zhu, Ziying Xie, Ming Zhang

{"title":"KIF14-AKT axis regulates ferroptosis sensitivity in triple-negative breast cancer.","authors":"Chunyu Shi, Weiyi Fang, Yingqi Zhu, Ziying Xie, Ming Zhang","doi":"10.1515/biol-2025-1324","DOIUrl":"https://doi.org/10.1515/biol-2025-1324","url":null,"abstract":"Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype with limited targeted treatment options. Ferroptosis has emerged as a potential therapeutic vulnerability in TNBC, but its upstream regulation remains incompletely defined. Here, we combined bioinformatics analysis with cell-based experiments to investigate the role of kinesin family member 14 (KIF14) in ferroptosis sensitivity. KIF14 was upregulated in TNBC datasets and associated with poor prognosis. In MDA-MB-468 cells, KIF14 knockdown reduced cell viability, increased malondialdehyde and intracellular Fe2+ levels, and induced mitochondrial changes consistent with ferroptosis. These effects were partially reversed by ferrostatin-1. Comparison with inhibitors of apoptosis, necroptosis, and pyroptosis showed that only ferrostatin-1 produced a clear rescue effect, supporting a predominantly ferroptosis-associated phenotype. Mechanistically, KIF14 depletion reduced phosphorylation of AKT serine/threonine kinase (AKT) and altered ferroptosis-related proteins, including decreased glutathione peroxidase 4 (GPX4) and solute carrier family 7 member 11 (SLC7A11) and increased acyl-CoA synthetase long-chain family member 4 (ACSL4). The AKT activator SC79 partially reversed these biochemical and phenotypic changes. Reciprocal co-immunoprecipitation further supported an association between endogenous KIF14 and AKT. In addition, KIF14 knockdown had minimal effects on the viability of normal MCF-10A cells. Together, these findings support a functional link between KIF14, AKT signaling, and ferroptosis sensitivity in TNBC.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251324"},"PeriodicalIF":1.7,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functionalized chitosan-graphene magnetic nanocomposites enhance collagen synthesis in scleral fibroblasts. 功能化壳聚糖-石墨烯磁性纳米复合材料增强巩膜成纤维细胞胶原合成。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2026-04-30 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1316

Xiaojie Huang, Zijian Wang, Min Zhang, Yingying Dai, Ruikang Tian

{"title":"Functionalized chitosan-graphene magnetic nanocomposites enhance collagen synthesis in scleral fibroblasts.","authors":"Xiaojie Huang, Zijian Wang, Min Zhang, Yingying Dai, Ruikang Tian","doi":"10.1515/biol-2025-1316","DOIUrl":"https://doi.org/10.1515/biol-2025-1316","url":null,"abstract":"To investigate the effects of magnetic stimulation mediated by magnetic/graphene (MNP/G) composite nanomaterials on human scleral fibroblast (HFSF) proliferation and collagen synthesis, exploring its potential for myopia prevention. MNP/G nanomaterials were synthesized via covalent co-precipitation and introduced into HFSF cultures. Cells were co-cultured with 30 μg/mL MNP/G and exposed to static magnetic stimulation (20 mT intensity). Proliferation was assessed via CCK-8 assay, morphology was observed using inverted phase-contrast microscopy, and hydroxyproline content was measured to quantify collagen synthesis. Magnetic stimulation significantly enhanced HFSF proliferation compared to non-stimulated controls, with no observed cytotoxicity. Microscopic analysis revealed improved cell density and spindle-like morphology in stimulated groups. Hydroxyproline assays demonstrated a marked increase in collagen synthesis following magnetic stimulation, suggesting enhanced extracellular matrix production. Notably, the combined application of MNP/G nanomaterials and magnetic stimulation yielded the highest collagen content among all experimental conditions. Magnetic stimulation using MNP/G nanomaterials promotes HFSF proliferation and collagen production, potentially strengthening scleral biomechanical properties. This approach may counteract scleral remodeling in myopia progression, offering a novel strategy for non-invasive myopia intervention.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251316"},"PeriodicalIF":1.7,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147818634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating bulk RNA-seq and ScRNA-seq to identify manganese metabolism-related subtypes and immunoregulatory mechanisms in liver hepatocellular carcinoma. 整合bulk RNA-seq和ScRNA-seq鉴定肝细胞癌中锰代谢相关亚型和免疫调节机制

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2026-04-29 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1298

Chunhui Liu, Wenqi Zhang, Wenzi Luo, Caifeng Zhang, Bo Zhang, Guozhi Zhang, Xiaotao Wang, Jianli Chen, Han Zhou

{"title":"Integrating bulk RNA-seq and ScRNA-seq to identify manganese metabolism-related subtypes and immunoregulatory mechanisms in liver hepatocellular carcinoma.","authors":"Chunhui Liu, Wenqi Zhang, Wenzi Luo, Caifeng Zhang, Bo Zhang, Guozhi Zhang, Xiaotao Wang, Jianli Chen, Han Zhou","doi":"10.1515/biol-2025-1298","DOIUrl":"https://doi.org/10.1515/biol-2025-1298","url":null,"abstract":"Liver hepatocellular carcinoma (LIHC) is an aggressive cancer associated with chronic liver disease, necessitating better biomarkers and therapies. Manganese, an essential trace element, regulates tumor development. Data from TCGA and GEO databases were analyzed to identify manganese metabolism-related genes (MMRGs). LIHC samples were classified into subtypes via consensus clustering. A prognostic model was developed using LASSO and multivariate Cox regression, then validated using ROC and survival analysis. Immune infiltration was assessed via ssGSEA and CIBERSORT, and cell communication was analyzed with single-cell data (GSE149614). Tumor Mutational Burden (TMB), drug sensitivity, and a nomogram were also evaluated. Two manganese metabolism-related subtypes were identified, with Cluster 1 showing better survival. A two-gene model (CEP55 and SPP1) reliably predicted poor prognosis in high-risk groups. The high-risk group exhibited distinct immune profiles, including increased immune infiltration, elevated checkpoint expression, and higher TIDE scores. Single-cell analysis revealed altered T cell communication. This study established manganese metabolism-related subtypes and a prognostic model for LIHC, providing insights into immunoregulation and cell communication to guide precision diagnosis and immunotherapy.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251298"},"PeriodicalIF":1.7,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13127686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147818642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Donkey serum albumin improves cyclophosphamide-induced anemia in mice. 驴血清白蛋白改善小鼠环磷酰胺性贫血。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2026-04-29 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1262

Nan Qin, Yuwang Cao, Ruiqi Xu, Xinyuan Liu, Guofang Zheng, Lulu Song, Rui Zhang, Yunfei Li, Lixia Xing

{"title":"Donkey serum albumin improves cyclophosphamide-induced anemia in mice.","authors":"Nan Qin, Yuwang Cao, Ruiqi Xu, Xinyuan Liu, Guofang Zheng, Lulu Song, Rui Zhang, Yunfei Li, Lixia Xing","doi":"10.1515/biol-2025-1262","DOIUrl":"https://doi.org/10.1515/biol-2025-1262","url":null,"abstract":"Cyclophosphamide (CP) is commonly used in cancer chemotherapy and the treatment of autoimmune diseases. In clinical applications, it exhibits the side effect of progressive anemia. Donkey serum albumin (DSA) is the most abundant protein component in Asini Corii Colla (ACC). This experiment aims to investigate the alleviating effect of donkey serum albumin on CP-induced anemia. 60 mice were randomly divided into six groups: normal control group (NC), model control group (MC), positive control group (PC), low-dose DSA (DSA-L, 75 mg/kg/d), medium-dose DSA (DSA-M, 150 mg/kg/d), and high-dose DSA (DSA-H, 300 mg/kg/d), with 10 mice in each group. An anemia model was established by intraperitoneal injection of CP. The treatment continued for 21 days. Afterward, the mice were euthanized, and histopathological examinations of bone marrow and spleen tissues were conducted. Organ indices, the number of bone marrow nucleated cells, and the levels of Erythropoietin (EPO), Thrombopoietin (TPO), and Vascular Endothelial Growth Factor (VEGF) in mouse serum and bone marrow supernatant were measured using ELISA. The model group exhibited significantly lower thymus index, White Blood Cell count (WBC), Red Blood Cell count (RBC),Hemoglobin (HGB), Platelet count (PLT), and bone marrow nucleated cell count (P < 0.01), as well as significantly reduced levels of EPO, TPO, and VEGF in serum and bone marrow supernatant (P < 0.01). Additionally, obvious tissue damage was observed in the bone marrow and spleen of the model group. In contrast, all DSA dose groups showed significant improvements in these indices compared to the model group (P < 0.05, P < 0.01), along with notable amelioration of histopathological damage in the bone marrow and spleen. Donkey serum albumin significantly improves hematological function in mice with CP-induced anemia.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251262"},"PeriodicalIF":1.7,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147818616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0