Open Life Sciences最新文献

Anti-arthritic, immunomodulatory, and inflammatory regulation by the benzimidazole derivative BMZ-AD: Insights from an FCA-induced rat model. 苯并咪唑衍生物BMZ-AD的抗关节炎、免疫调节和炎症调节作用：来自fca诱导大鼠模型的见解

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-06-17 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1083

Haseeb Ahmad, Irfan Anjum, Halima Usman, Aisha Mobashar, Arham Shabbir, Yousef A Bin Jardan, Amira Metouekel, Musaab Dauelbait, Mohammed Bourhia

{"title":"Anti-arthritic, immunomodulatory, and inflammatory regulation by the benzimidazole derivative BMZ-AD: Insights from an FCA-induced rat model.","authors":"Haseeb Ahmad, Irfan Anjum, Halima Usman, Aisha Mobashar, Arham Shabbir, Yousef A Bin Jardan, Amira Metouekel, Musaab Dauelbait, Mohammed Bourhia","doi":"10.1515/biol-2025-1083","DOIUrl":"10.1515/biol-2025-1083","url":null,"abstract":"Rheumatoid arthritis (RA) is an autoimmune disease causing joint inflammation, deformity, cartilage deterioration, and pain. Benzimidazole derivatives exhibit various pharmacological properties. This study evaluated the antiarthritic, immunomodulatory, and anti-inflammatory potential of a benzimidazole derivative 2-(2-(benzylthio)-1H-benzo[d]imidazol-1-yl)-N'-(4-nitrobenzylidiene) acetohydrazide (BMZ-AD) in a Freund's complete adjuvant (FCA)-induced arthritic rat model. FCA was administered on day 0, and treatment with BMZ-AD (25 mg/kg, 50 mg/kg, and 75 mg/kg) and piroxicam (10 mg/kg) began on day 7 and continued up to 28 days. Rats were sacrificed on day 28. Arthritis was assessed using an arthritic scoring index, and paw edema was measured with a digital water plethysmometer. Biochemical and hematological parameters were analyzed, reverse transcription polymerase chain reaction measured the mRNA expression of tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6), and molecular docking evaluated BMZ-AD interactions with these proteins. Enzyme-linked immunosorbent assay determined prostaglandin E2 levels. BMZ-AD treatment reduced inflammation, pannus formation, and pro-inflammatory cytokines (TNF-α and IL-6) and decreased PGE2 levels, comparable to piroxicam. Blood profiles improved with significant reductions in white blood cells and platelets in treatment groups. BMZ-AD demonstrated antiarthritic, anti-inflammatory, and immunomodulatory properties, suggesting that it could be a potential drug for RA treatment with fewer side effects.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251083"},"PeriodicalIF":1.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenolic spectrum of cornelian cherry fruits and their health-promoting effect. 山茱萸果实的多酚光谱及其保健作用。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-06-17 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1117

Tunde Jurikova, Nela Skowronkova, Magdalena Zvonkova, Jana Orsavova, Sezai Ercisli, Libor Dokoupil, Katarina Fatrcova-Sramkova, Anna Adamkova, Jiri Mlcek

引用次数: 0

Research progress on the regulation of autophagy in cardiovascular diseases by chemokines. 趋化因子调控心血管疾病自噬的研究进展。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-06-17 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2022-1026

Jingfeng Ma, Xiaotong Tan, Juling Feng, Zhihui Li, Shuo Tan, Boling Li, Lei Zhao

引用次数: 0

Hesperidin and its zinc(ii) complex enhance osteoblast differentiation and bone formation: In vitro and in vivo evaluations. 橙皮苷及其锌复合物增强成骨细胞分化和骨形成：体外和体内评价。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-06-17 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2022-1032

Pan Li, Jing Wang, Huan Wang, Songchun Liu, Qibin Zhang

{"title":"Hesperidin and its zinc(ii) complex enhance osteoblast differentiation and bone formation: In vitro and in vivo evaluations.","authors":"Pan Li, Jing Wang, Huan Wang, Songchun Liu, Qibin Zhang","doi":"10.1515/biol-2022-1032","DOIUrl":"10.1515/biol-2022-1032","url":null,"abstract":"This investigation explores the impact of hesperidin and its zinc(ii) complex on osteoblast differentiation and subsequent bone formation. The biocompatibility of synthesized complexes (0-20 μg/mL) was assessed in vitro using mouse mesenchymal stem cells, while in vivo toxicity was evaluated using a chick embryo model. Both hesperidin and its zinc(ii) complex were found to be non-toxic at a concentration of 10 μg/mL. Notably, these compounds significantly increased alkaline phosphatase activity and enhanced calcium deposition. Molecular analyses revealed upregulation of Runx2 and type 1 collagen mRNA expression, along with increased levels of osteonectin and osteocalcin proteins, while negative regulators of osteoblast differentiation (Smad7, Smurf1, HDAC7) were downregulated. A new aspect of this study is demonstrating that the zinc(ii) complex of hesperidin uniquely enhances osteogenic activity compared to hesperidin alone, highlighting its potential to improve bone formation significantly. Additionally, we elucidated the role of miR-143-3p in mediating these effects, achieved through HDAC7 suppression and enhanced Runx2 expression, assessed using the pmirGLO dual luciferase reporter system. Zebrafish studies further demonstrated the complexes' effects on bone formation, revealing increased osteoblastic activity and improved calcium-to-phosphorus ratios in regenerated scales. These findings underscore the potential of hesperidin-Zn(ii) as a promising therapeutic agent for bone tissue engineering.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221032"},"PeriodicalIF":1.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Piriformospora indica and arbuscular mycorrhizal fungus on growth and physiology of Moringa oleifera under low-temperature stress. 低温胁迫下梨形孢子菌和丛枝菌根真菌对辣木生长和生理的影响。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-06-13 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1111

Guo Zhou, Sini Wu, Meichun Qiu, Yingtong Long, Qian He, Junjie Zhang

{"title":"Effects of Piriformospora indica and arbuscular mycorrhizal fungus on growth and physiology of Moringa oleifera under low-temperature stress.","authors":"Guo Zhou, Sini Wu, Meichun Qiu, Yingtong Long, Qian He, Junjie Zhang","doi":"10.1515/biol-2025-1111","DOIUrl":"10.1515/biol-2025-1111","url":null,"abstract":"Moringa is a perennial tree with high nutritional value, and it is drought tolerant and barren but has poor cold resistance during the seedling stage. This study selected Piriformospora indica (PI) and arbuscular mycorrhizal fungus (AM) as inoculants, Moringa seedlings were inoculated, and their growth and physiological responses were evaluated under different low-temperature stress times. The research results show that PI and AM can symbiotically associate with Moringa successfully and promote their nutritional growth. At low temperature, Moringa inoculated with PI and AM exhibited better physiological resistance. However, the effect of mixed inoculation of PI and AM is not as significant as that of single inoculation of any strain. Inoculating plant growth-promoting rhizobia (PGPR) reduced the richness of fungal communities and the number of unique operational taxonomic units (OTUs), with PI being the most prominent. Vaccination with PGPR also increased bacterial diversity, richness, and the number of unique OTUs, with AM inoculation showing the most prominent performance. This suggests that Moringa seedling growth and responsiveness to low-temperature stress are significantly influenced by PGPR, and there may be interactions between different bacterial strains. The results suggest that PGPR can improve the yield and quality of Moringa by promoting growth and regulating stress resistance.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251111"},"PeriodicalIF":1.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A de novo meningioma with rapid growth: A possible malignancy imposter? 新生快速生长的脑膜瘤：可能是恶性肿瘤的骗子？

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-06-12 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1118

Zhenjiang Pan, Jing Bao, Shepeng Wei

{"title":"A de novo meningioma with rapid growth: A possible malignancy imposter?","authors":"Zhenjiang Pan, Jing Bao, Shepeng Wei","doi":"10.1515/biol-2025-1118","DOIUrl":"10.1515/biol-2025-1118","url":null,"abstract":"Meningiomas, accounting for approximately 33% of primary central nervous system tumors, are the most prevalent type in this category. Advanced age is frequently viewed as a barrier to surgical intervention, yet recent cases have challenged this perception by demonstrating successful outcomes in elderly patients. This case report aims to illustrate the feasibility and benefits of surgical treatment in older individuals. An 84-year-old patient presented with a newly diagnosed meningioma and underwent surgical tumor removal at age 88. Following a comprehensive preoperative evaluation that excluded significant comorbidities, the procedure utilized advanced surgical techniques and optimized postoperative care to ensure safety and recovery. Pathology revealed a World Health Organization grade 1 fibrous meningioma, confirming its benign nature. The patient tolerated the surgery well and recovered successfully, marking her as the oldest reported individual to undergo such treatment. This case demonstrates that advanced age does not inherently limit tumor growth or preclude surgical intervention. Through meticulous patient assessment and personalized treatment strategies, elderly patients can achieve positive outcomes. It highlights the value of a tailored approach, prioritizing overall health and specific medical needs, and supports proactive surgical management to enhance quality of life and clinical results. This challenges traditional assumptions about age-related restrictions on surgical feasibility.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251118"},"PeriodicalIF":1.7,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144507339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosis of secondary tuberculosis infection in an asymptomatic elderly with cancer using next-generation sequencing: Case report. 使用下一代测序诊断无症状老年癌症继发结核感染：病例报告。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-06-12 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1123

Jiahuan Huang, Wenbo Ren, Weishang Hu, Jianfang Ni

引用次数: 0

Oncolytic bacteria: A revolutionary approach to cancer therapy. 溶瘤细菌：一种革命性的癌症治疗方法。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-06-10 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1076

Mohsen A Khormi, Saleh M Al-Maaqar, Abdulaziz R Al Johni, Nasser A Al-Tayyar, Jafar Abdullah Alhamad, Abdullah A Ghyathuddin, Zakia Alblawi, Sabreen M Behairy, Mohammed A Alghamdi, Wael A Alsubhi, Mikiyas D Teklemariam

{"title":"Oncolytic bacteria: A revolutionary approach to cancer therapy.","authors":"Mohsen A Khormi, Saleh M Al-Maaqar, Abdulaziz R Al Johni, Nasser A Al-Tayyar, Jafar Abdullah Alhamad, Abdullah A Ghyathuddin, Zakia Alblawi, Sabreen M Behairy, Mohammed A Alghamdi, Wael A Alsubhi, Mikiyas D Teklemariam","doi":"10.1515/biol-2025-1076","DOIUrl":"10.1515/biol-2025-1076","url":null,"abstract":"Cancer is one of the most devastating diseases all over the globe, and it is the second worldwide cause of death, exceeded only by cardiovascular diseases. The therapeutic approach to human cancer has evolved significantly and has varied depending on the type and stage of cancer, as well as the general health status of the patient. Despite the advancements in cancer treatment, various challenges persist in the treatment of cancer, including side effects, drug resistance, and incomplete eradication of tumors. The use of oncolytic bacteria (cancer targeting and destroying bacteria) has been identified to have several advantages over the traditional methods of cancer treatment. Several bacterial species have been identified to be used in the treatment of different types of cancers. Oncolytic therapy can be achieved through the use of a naturally occurring and/or genetically modified bacterial species, including Clostridium, Salmonella, Escherichia coli, and Listeria spp. with their toxins, enzymes, biofilms, and secondary metabolites as well as their spores that leads to direct or indirect killing of cancer cells. This review provides some highlights about the biology and therapeutic potential of oncolytic bacteria individually or in combination with other therapeutic approaches against different types of cancers. Besides, the current challenges and future perspectives will be explored.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251076"},"PeriodicalIF":1.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CEBPB promotes ulcerative colitis-associated colorectal cancer by stimulating tumor growth and activating the NF-κB/STAT3 signaling pathway. CEBPB通过刺激肿瘤生长和激活NF-κB/STAT3信号通路促进溃疡性结肠炎相关结直肠癌的发生。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-06-06 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2022-1012

Shan Gao, Wei Wang, Xu-Dong Tong, Dao-Rong Wang, Ai-Xia Tian, Wei Yang, Jin-Min Chen

{"title":"CEBPB promotes ulcerative colitis-associated colorectal cancer by stimulating tumor growth and activating the NF-κB/STAT3 signaling pathway.","authors":"Shan Gao, Wei Wang, Xu-Dong Tong, Dao-Rong Wang, Ai-Xia Tian, Wei Yang, Jin-Min Chen","doi":"10.1515/biol-2022-1012","DOIUrl":"10.1515/biol-2022-1012","url":null,"abstract":"Ulcerative colitis-associated colorectal cancer (UCCRC) represents a significant complication of ulcerative colitis. CEBPB has been shown to promote the invasion of colon cancer cells. In this study, we aimed to investigate the role of CEBPB in the progression of cancers associated with colitis. The wild-type (WT) mice, transfected with a vector expressing CEBPB and siRNA targeting CEBPB, along with their littermate controls, were subjected to a challenge using azoxymethane and dextran sodium sulfate to establish a model of UCCRC. Colon tissues and blood samples were collected for analysis through hematoxylin and eosin staining and enzyme linked immunosorbent assay. Immunohistochemical staining was employed to assess protein expression. In the UCCRC model, mice transfected with vectors expressing CEBPB exhibited a reduction in weight loss and colorectal stenosis, as well as disordered colonic gland structure. Additionally, these mice demonstrated an increased number and size of tumors compared to WT controls. Furthermore, transfection with CEBPB resulted in a decrease in both the quantity and dimensions of tumors. NF-κB was found to enhance the phosphorylation level of STAT3 based on Western blot assay. The activation of NF-κB and STAT3 subsequently promoted the proliferation, invasion, and migration of colon cancer cells by clone formation assays, transwell assays, and scratch-wound assays. Moreover, rescue experiments indicated that CEBPB induced UCCRC through the NF-κB/STAT3 signaling pathway. CEBPB mediated colonic injury in UCCRC mice by activating the NF-κB/STAT3 pathway. This finding reveals a previously unrecognized link between CEBPB and colitis-related tumorigenesis and provides new insight into UCCRC pathogenesis.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221012"},"PeriodicalIF":1.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on individualized drug sensitivity detection technology based on bio-3D printing technology for precision treatment of gastrointestinal stromal tumors. 基于生物3d打印技术的个体化药敏检测技术在胃肠道间质瘤精准治疗中的研究。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-05-27 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1108

Yang-Yang Tu, Zhong-Ting Huang, Zhi-Yong Zhang, Dong-Liang Liu, Hai-Long Qian

{"title":"Research on individualized drug sensitivity detection technology based on bio-3D printing technology for precision treatment of gastrointestinal stromal tumors.","authors":"Yang-Yang Tu, Zhong-Ting Huang, Zhi-Yong Zhang, Dong-Liang Liu, Hai-Long Qian","doi":"10.1515/biol-2025-1108","DOIUrl":"10.1515/biol-2025-1108","url":null,"abstract":"This study aims to analyze and explore whether tumor biological three-dimensional printing (3DP) models can serve as reliable preclinical model research tools and assist in the personalized treatment of gastrointestinal stromal tumor (GIST) patients. Ten GIST cases admitted to our hospital from May 2024 to September 2024 were included in the personalized treatment group. Patient-derived GIST 3DP models were established, and treatment plans were selected based on the results of drug sensitivity tests. The progression-free survival (PFS) of the personalized treatment group was compared with that of GIST patients who had progressed after first-line treatment and were admitted to our hospital before the study. Treatment safety was also assessed. Immunofluorescence staining technology was used to observe tumor markers in the 3DP tumor models and their corresponding parent tumor tissues, revealing a high degree of consistency, which indicates that the 3DP tumor models highly retain the histological characteristics of the parent tumor tissues. The median PFS of patients in the personalized treatment group was 6.1 months, compared to 5.3 months in the previous treatment group, with a statistically significant difference (P-value <0.05). The individualized drug sensitivity detection technology based on bio-3D printing technology, used for the personalized treatment of GIST patients who have progressed after first-line treatment, can benefit patients.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251108"},"PeriodicalIF":1.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12120404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0