Open Life Sciences最新文献

Kynurenine facilitates renal cell carcinoma progression by suppressing M2 macrophage pyroptosis through inhibition of CASP1 cleavage. 犬尿氨酸通过抑制CASP1裂解抑制M2巨噬细胞焦亡，促进肾癌进展。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-10-08 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1174

Wenmao Huang, Jingxuan Chen

{"title":"Kynurenine facilitates renal cell carcinoma progression by suppressing M2 macrophage pyroptosis through inhibition of CASP1 cleavage.","authors":"Wenmao Huang, Jingxuan Chen","doi":"10.1515/biol-2025-1174","DOIUrl":"10.1515/biol-2025-1174","url":null,"abstract":"Renal cell carcinoma (RCC) is an aggressive malignancy with a poor prognosis influenced by pyroptosis in tumor-associated M2 macrophages. This study investigated how kynurenine modulates pyroptosis in M2 macrophages and promotes RCC progression. M2 macrophages were treated with pyroptosis inhibitor VX-765 or kynurenine to evaluate their effects on cell viability and pyroptosis. Transwell co-culture systems were employed to assess the impact of M2 macrophages on RCC cell proliferation, colony formation, and viability. The interaction between kynurenine and CASP1 (caspase-1), a key executor of pyroptosis that cleaves gasdermin D (GSDMD) to trigger inflammatory cell death, was analyzed using surface plasmon resonance. The results demonstrated that VX-765 treatment significantly enhanced M2 macrophage viability while reducing pyroptosis, thereby promoting RCC cell proliferation in co-culture systems. Kynurenine significantly enhanced M2 macrophage viability while suppressing pyroptosis. Mechanistically, kynurenine reduced the cleavage of CASP1 (caspase-1) by directly binding to it. Overexpression of CASP1 reversed kynurenine-induced suppression of pyroptosis in M2 macrophages. Furthermore, CASP1 overexpression abolished kynurenine-mediated enhancement of RCC cell viability, colony formation, and proliferation. This study revealed that kynurenine inhibits pyroptosis in M2 macrophages via direct targeting of CASP1, creating a tumor-supportive microenvironment that accelerates RCC progression. These findings establish the kynurenine-CASP1 axis as a critical regulator of M2 macrophage pyroptosis and demonstrate its role in promoting RCC progression, identifying a potential therapeutic target for RCC treatment.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251174"},"PeriodicalIF":1.7,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12514781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ALKBH5 exacerbates early cardiac damage after radiotherapy for breast cancer via m6A demethylation of TLR4. ALKBH5通过TLR4的m6A去甲基化加剧乳腺癌放疗后早期心脏损伤。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-10-08 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1184

Xiaokeya Yasen, Yilinuer Maihesumu, Dilixiati Wusiman, Abudula Aihemaiti, Xirmaimaiti Aishan, Munire Mushajiang

{"title":"ALKBH5 exacerbates early cardiac damage after radiotherapy for breast cancer via m6A demethylation of TLR4.","authors":"Xiaokeya Yasen, Yilinuer Maihesumu, Dilixiati Wusiman, Abudula Aihemaiti, Xirmaimaiti Aishan, Munire Mushajiang","doi":"10.1515/biol-2025-1184","DOIUrl":"https://doi.org/10.1515/biol-2025-1184","url":null,"abstract":"Radiotherapy is an important cancer treatment for breast cancer patients, improving overall survival; however, it can lead to a common complication, radiation-induced heart disease (RIHD). N6-methyladenosine (m6A) RNA modification plays a critical role in the regulation of myocardial function. The aim of this study is to investigate the effect of m6A modification on cardiac injury following radiotherapy for breast cancer. Cardiac dysfunction was assessed by echocardiography and hematoxylin and eosin staining. The expression of ALKBH5 was analyzed by quantitative real-time PCR, western blot, and immunohistochemistry. The underlying mechanism was investigated using methylated RNA immunoprecipitation (MeRIP), RIP, and dual-luciferase reporter assays. The results showed that in RIHD, ALKBH5 expression was upregulated in breast cancer patients after radiotherapy and in RIHD mouse models. ALKBH5 downregulated the m6A modification level of Toll-like receptor 4 (TLR4). Overexpression of TLR4 abolished the inhibitory effect of ALKBH5 silencing on RIHD in mice. In summary, this study revealed a novel regulatory mechanism of ALKBH5-mediated m6A demethylation in RIHD, which could provide a promising therapeutic strategy for cardiac dysfunction following radiotherapy in breast cancer patients.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251184"},"PeriodicalIF":1.7,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12514778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RFX5 promotes the growth, motility, and inhibits apoptosis of gastric adenocarcinoma cells through the SIRT1/AMPK axis. RFX5通过SIRT1/AMPK轴促进胃腺癌细胞生长、运动、抑制凋亡。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-10-08 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1176

Lili Zhu, Le Qi

{"title":"RFX5 promotes the growth, motility, and inhibits apoptosis of gastric adenocarcinoma cells through the SIRT1/AMPK axis.","authors":"Lili Zhu, Le Qi","doi":"10.1515/biol-2025-1176","DOIUrl":"https://doi.org/10.1515/biol-2025-1176","url":null,"abstract":"Gastric cancer is among the most common gastrointestinal malignancies with high morbidity and mortality rates, highlighting the need to further elucidate its pathogenesis and identify effective therapeutic targets. The regulatory factor X (RFX) gene family encodes transcription factors implicated in the development and progression of several cancers. Although RFX5 has been reported to influence tumor progression in various malignancies, its specific role in gastric adenocarcinoma remains unclear. In this study, we investigated the functional effects of RFX5 in gastric adenocarcinoma. Our findings revealed that RFX5 is highly expressed in gastric adenocarcinoma tissues. Silencing of RFX5 significantly inhibited cell proliferation and migration, while promoting apoptosis in gastric adenocarcinoma cells. Mechanistically, RFX5 knockdown activated the silent information regulator transcript 1/adenosine monophosphate-activated protein kinase (SIRT1/AMPK) signaling axis. These results suggest that RFX5 facilitates the growth and motility of gastric adenocarcinoma cells and suppresses apoptosis, at least in part, through modulation of the SIRT1/AMPK pathway.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251176"},"PeriodicalIF":1.7,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12514779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative bioinformatics analysis of the Wnt pathway in breast cancer: Selection of novel biomarker panels associated with ER status. 乳腺癌Wnt通路的比较生物信息学分析：选择与ER状态相关的新型生物标志物面板。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-10-08 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1173

Klaudia Waszczykowska, Damian Kołat, Żaneta Kałuzińska-Kołat, Elżbieta Płuciennik

{"title":"Comparative bioinformatics analysis of the Wnt pathway in breast cancer: Selection of novel biomarker panels associated with ER status.","authors":"Klaudia Waszczykowska, Damian Kołat, Żaneta Kałuzińska-Kołat, Elżbieta Płuciennik","doi":"10.1515/biol-2025-1173","DOIUrl":"10.1515/biol-2025-1173","url":null,"abstract":"Breast cancer (BC) is a major global health concern, ranking among the most common neoplasms and representing one of the leading causes of cancer-related deaths worldwide. Early recognition and classification of BC subtypes are crucial for improving patient outcomes. Therefore, identifying novel biomarkers with diagnostic and prognostic significance is of great importance. The Wnt signaling pathway plays a significant role in BC by influencing various cell cycle regulation processes and stem cell renewal. This study aims to identify novel Wnt-associated biomarker panels for BC patients, composed of multiple molecular factors. A series of bioinformatical analyses have been employed, including weighted gene co-expression network analysis, differential expression analysis, Kaplan-Meier survival analysis, logistic regression model evaluation, and receiver operating characteristic construction. Thus, this study revealed potential diagnostic and prognostic signatures based on comprehensive analyses of BC patient data sourced from The Cancer Genome Atlas database. Consequently, four gene signatures were constructed: two differentiate ER+ from ER-BC: TTC8, SLC5A7, and PLCH1 for overall survival (OS); ZNF695, SLC7A5, and PLCH1 for disease free survival (DFS), while the other two effectively distinguish tumor from normal samples: SPC25, ANLN, KPNA2, SLC7A5 for OS; SPC25, KIF20A, SKA3, DTL, CDCA3, ANLN, TTK, RAD54L, MYBL2, ZNF695, and SLC7A5 for DFS.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251173"},"PeriodicalIF":1.7,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12514780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treatment of pure red cell aplasia in a chronic kidney disease patient with roxadustat: A case report. 罗沙司他治疗慢性肾病患者纯红细胞发育不全1例。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-10-04 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1155

Shanlin Liu, Bonan Yan, Yuhua He

{"title":"Treatment of pure red cell aplasia in a chronic kidney disease patient with roxadustat: A case report.","authors":"Shanlin Liu, Bonan Yan, Yuhua He","doi":"10.1515/biol-2025-1155","DOIUrl":"https://doi.org/10.1515/biol-2025-1155","url":null,"abstract":"Pure red cell aplasia (PRCA) is a rare blood disorder that is characterized by severe hypo-erythroid bone marrow hypoplasia leading to severe anemia that usually does not respond to standard treatment. In patients with chronic kidney disease (CKD), especially in those patients who are erythropoiesis-stimulating agent (ESA) resistant, management can be quite difficult. In this case report, we will present a 67-year-old woman with CKD (not on dialysis) who presented with refractory anemia (hemoglobin 45-65 g/L) and was ultimately diagnosed with PRCA after bone marrow aspirate. This patient had previously received treatment (ESAs, iron supplementation, and multiple blood transfusions) without improvement in her blood counts. After initiation of oral Roxadustat 100 mg t.i.w., her hemoglobin increased gradually and stabilized between 100 and 106 g/L, and she no longer required blood transfusions. This case report highlights the potential role of Roxadustat as a new therapeutic option for PRCA and ESA-resistant CKD. While these data are encouraging, larger controlled studies are going to be required to evaluate measures of efficacy, dosing, and long-term safety in this population.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251155"},"PeriodicalIF":1.7,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12514861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bee venom promotes exosome secretion and alters miRNA cargo in T cells. 蜂毒促进外泌体分泌并改变T细胞中的miRNA载货。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-10-03 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1180

Ziyan Cui, Zegao Zhou, Ziyan Sun, Jiayue Duan, Runtian Liu, Cheng Qi, Changqing Yan

{"title":"Bee venom promotes exosome secretion and alters miRNA cargo in T cells.","authors":"Ziyan Cui, Zegao Zhou, Ziyan Sun, Jiayue Duan, Runtian Liu, Cheng Qi, Changqing Yan","doi":"10.1515/biol-2025-1180","DOIUrl":"10.1515/biol-2025-1180","url":null,"abstract":"Exosomes are important mediators of intercellular communication, primarily through the transfer of miRNAs. However, the mechanisms regulating exosome secretion and miRNA cargo loading in T cells remain incompletely understood. In this study, we investigated the effects of bee venom (BV), a natural compound with known immunomodulatory activity, on the exosomal miRNA profile in human Jurkat T cells. The non-cytotoxic concentration of BV (2 μg/mL) was identified using the CCK-8 assay. Exosomes were isolated from BV-treated and control cells and characterized by transmission electron microscopy, Western blotting, and nanoparticle tracking analysis (NTA), with NTA also used to quantify particle concentration for assessing changes in secretion levels. High-throughput sequencing identified 74 differentially expressed miRNAs (44 upregulated, 30 downregulated), which were validated by quantitative real-time PCR. Functional enrichment analyses (gene ontology, kyoto encyclopedia of genes and genomes, disease ontology, Reactome) revealed associations with pathways related to neural development, cell cycle regulation, and tumorigenesis. BV treatment significantly promoted exosome release and selectively altered miRNA cargo. These findings suggest that BV modulates T cell-derived exosome output and composition, providing mechanistic insights into how it may influence immune-related signaling pathways.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251180"},"PeriodicalIF":1.7,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12514777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Effects of Ire1 gene on virulence and pathogenicity of Candida albicans". “Ire1基因对白色念珠菌毒力和致病性的影响”的勘误表。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-09-30 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-0002

Huihai Zhao, Lixia Qin, Mengyan Li, Mengyu Jiang, Mengge Cui, Hua Wang, Baohua Hou, Fukun Wang, Keran Jia

引用次数: 0

Novel cuproptosis-related genes C1QBP and PFKP identified as prognostic and therapeutic targets in lung adenocarcinoma. 新的铜肺相关基因C1QBP和PFKP被确定为肺腺癌的预后和治疗靶点。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-09-30 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1142

Yanju Lv, Xiaozhuo Duan, Xueli Yuan

{"title":"Novel cuproptosis-related genes C1QBP and PFKP identified as prognostic and therapeutic targets in lung adenocarcinoma.","authors":"Yanju Lv, Xiaozhuo Duan, Xueli Yuan","doi":"10.1515/biol-2025-1142","DOIUrl":"10.1515/biol-2025-1142","url":null,"abstract":"Excessive intracellular copper accumulation triggers cuproptosis, a novel regulated cell death process with therapeutic potential. Analyzing 566 The Cancer Genome Atlas samples alongside lung adenocarcinoma (LUAD)-specific microarray and single-cell sequencing data, we identified 109 cuproptosis-associated genes, of which C1QBP and PFKP emerged as key prognostic markers. Four-gene risk model stratified patients into high- and low-risk groups with distinct survival outcomes, where high-risk scores correlated with advanced TNM stages. Clinical validation confirmed that elevated C1QBP/PFKP expression in LUAD tissues predicted shorter progression-free survival. Functional assays demonstrated that silencing C1QBP or PFKP increased intracellular copper concentration, suppressed proliferation, and inhibited invasion, mechanistically linking these genes to cuproptosis dysregulation. Our findings nominate C1QBP/PFKP as actionable targets for LUAD therapy, offering both prognostic biomarkers and copper-metabolism-directed treatment strategies.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251142"},"PeriodicalIF":1.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ADH5 inhibits proliferation but promotes EMT in non-small cell lung cancer cell through activating Smad2/Smad3. ADH5通过激活Smad2/Smad3抑制非小细胞肺癌细胞增殖，但促进EMT。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-09-23 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1148

Xinyu Tan, Ye Huang, Xiaolei Li, Fei Xu, Xinping Xu

{"title":"ADH5 inhibits proliferation but promotes EMT in non-small cell lung cancer cell through activating Smad2/Smad3.","authors":"Xinyu Tan, Ye Huang, Xiaolei Li, Fei Xu, Xinping Xu","doi":"10.1515/biol-2025-1148","DOIUrl":"https://doi.org/10.1515/biol-2025-1148","url":null,"abstract":"Cell proliferation and epithelial-mesenchymal transition (EMT) are two common tumor phenotypes closely linked to malignant tumor progression. Genes regulating tumor progression often exhibit consistent regulatory trends in these phenotypes; however, certain genes may display inconsistent regulatory patterns in tumor proliferation and EMT. In this investigation, initial transcriptomic and tumor database analyses revealed that alcohol dehydrogenase 5 (ADH5) is downregulated in non-small cell lung cancer (NSCLC) tissues and correlates negatively with NSCLC prognosis. Subsequent experimental manipulation of ADH5 levels in tumor cells demonstrated that ADH5 overexpression decreased proliferation while enhancing migration and invasion capacities in NSCLC cells. Moreover, ADH5 overexpression hindered xenograft tumor growth in nude mice. However, ADH5 knockdown yielded contrasting outcomes by stimulating NSCLC cell proliferation while impeding migration and invasion abilities. Notably, ADH5 overexpression triggered EMT through Smad2/Smad3 activation, leading to the upregulation of SRY-Box Transcription Factor 9. TGFbetaR1/ALK5 inhibitor SB431542 was able to alleviate the effects of ADH5 overexpression on NSCLC cells. This study indicates a critical role of ADH5 in tumors associated with cancer cell growth inhibition but EMT activation. These findings underscore ADH5 as a potential regulator of NSCLC cell plasticity, emphasizing its promise as a therapeutic target for NSCLC management.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251148"},"PeriodicalIF":1.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12514862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoptotic efficacies of AgNPs formulated by Syzygium aromaticum leaf extract on 32D-FLT3-ITD human leukemia cell line with PI3K/AKT/mTOR signaling pathway. 香参叶提取物制备AgNPs对具有PI3K/AKT/mTOR信号通路的32D-FLT3-ITD人白血病细胞的凋亡作用

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2025-09-23 eCollection Date: 2025-01-01 DOI: 10.1515/biol-2025-1161

Liang Guo, Ru Kou, Guang Li, Yanping Song, Yunjie Zhang

{"title":"Apoptotic efficacies of AgNPs formulated by Syzygium aromaticum leaf extract on 32D-FLT3-ITD human leukemia cell line with PI3K/AKT/mTOR signaling pathway.","authors":"Liang Guo, Ru Kou, Guang Li, Yanping Song, Yunjie Zhang","doi":"10.1515/biol-2025-1161","DOIUrl":"10.1515/biol-2025-1161","url":null,"abstract":"Clove, Syzygium aromaticum, is a medicinal plant from the Myrtaceae family with various applications in traditional medicine. The plant has been studied for its analgesic, anti-inflammatory, antiviral, and anticancer properties. This study focuses on the green synthesis of silver nanoparticles using clove leaf extract. The synthesized NPs were characterized using chemical methods and their anticancer activity was tested against a leukemia cell line, along with the signaling pathway that followed. The AgNPs were synthesized in a spherical shape and were less than 50 nm in size. The cytotoxic effects of the AgNPs on PCS-800-011 primary peripheral blood mononuclear cells and 32D-FLT3-ITD leukemia cells were evaluated over 48 h using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The cancer cells showed reduced viability with an IC50 value of 162 µg/mL after exposure to the AgNPs. Through a detailed examination of the mTOR pathway, it was observed that AgNPs can alter the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin pathway, affecting 32D-FLT3-ITD cell growth and death. This pathway may contribute to the inhibition of the cell cycle and induction of apoptosis by AgNPs. For this reason, AgNPs may be used as a natural anti-cancer treatment for leukemia.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251161"},"PeriodicalIF":1.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0