Open Life Sciences最新文献_第2页

The therapeutic potential of targeting Oncostatin M and the interleukin-6 family in retinal diseases: A comprehensive review. 靶向Oncostatin M和白细胞介素-6家族治疗视网膜疾病的潜力：综述

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2024-12-31 eCollection Date: 2024-01-01 DOI: 10.1515/biol-2022-1023

Tommaso Mori, Nareshkumar Ragavachetty Nagaraj, Pier Luigi Surico, Wenjing Zhou, Uday Pratap Singh Parmar, Fabiana D'Esposito, Caterina Gagliano, Mutali Musa, Marco Zeppieri

引用次数: 0

Role of circRMRP and circRPL27 in chronic obstructive pulmonary disease. circRMRP和circRPL27在慢性阻塞性肺疾病中的作用

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2024-12-31 eCollection Date: 2024-01-01 DOI: 10.1515/biol-2022-0942

JianFang Li, PengFei Zhang, XianJing Zeng, Rong Liu

{"title":"Role of circRMRP and circRPL27 in chronic obstructive pulmonary disease.","authors":"JianFang Li, PengFei Zhang, XianJing Zeng, Rong Liu","doi":"10.1515/biol-2022-0942","DOIUrl":"https://doi.org/10.1515/biol-2022-0942","url":null,"abstract":"Chronic obstructive pulmonary disease (COPD) is one of the leading causes of death and disability worldwide, and circRNA dysregulation is functionally associated with COPD. This study explored the potential of circRMRP and circRPL27 as biomarkers of COPD. Blood samples from COPD patients and healthy controls were collected. COPD patients were divided into mild, moderate, and severe groups according to lung function. Quantitative real-time polymerase chain reaction technology was used to determine the expression of circRPL27 and circRMRP in COPD. Receiver operating characteristic curve was drawn to explore the value of circRMRP and circRPL27 in diagnosing COPD. circRMRP and circRPL27 levels were elevated in serum of COPD patients and increased with the severity of COPD. CircRMRP and circRPL27 were associated with smoking history, WBC, and FEV1/FVC, and were positively correlated with smoking history and WBC, and negatively correlated with FEV1/FVC. In COPD, both circRMRP and circRPL27 had diagnostic values, but circRPL27 was better. circRMRP and circRPL27 may be useful non-invasive biomarkers for COPD diagnosis.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"19 1","pages":"20220942"},"PeriodicalIF":1.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the role of hyperexpressed HCN1 in inducing myocardial infarction through activation of the NF-κB signaling pathway. 探讨高表达HCN1通过激活NF-κB信号通路诱导心肌梗死的作用。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2024-12-31 eCollection Date: 2024-01-01 DOI: 10.1515/biol-2022-0967

Xiao Liang, Jie Zhang, Ya Luo

{"title":"Investigating the role of hyperexpressed HCN1 in inducing myocardial infarction through activation of the NF-κB signaling pathway.","authors":"Xiao Liang, Jie Zhang, Ya Luo","doi":"10.1515/biol-2022-0967","DOIUrl":"10.1515/biol-2022-0967","url":null,"abstract":"We investigated the protective effect of the NF-κB inhibitor, pyrrolidine dithiocarbamate (PDTC) on cardiomyocyte injury induced by HCN1 channel overexpression, and explored the underlying mechanisms. An HCN1 overexpression vector was constructed and transfected into H9C2 cells, followed by PDTC treatment. The experiments comprised the following groups: control, control + PDTC, overexpression negative control, HCN1 overexpression (HCN1-OE), and combined HCN1-OE + PDTC groups. Cell proliferation was assessed using the CCK8 assay, while apoptosis and reactive oxygen species (ROS) levels were measured by flow cytometry. ELISA kits were used to determine the levels of malondialdehyde, superoxide dismutase, and interleukin-1 beta. The HCN1-OE group exhibited increased apoptosis, elevated ROS, and decreased survival. Western blot (WB) analysis revealed increased levels of p65, p-IκB, IKKβ, NLRP3, Beclin-1, and LC3 II/I proteins in the HCN1-OE group. PDTC treatment for 48 h post-HCN1-OE resulted in improved cell viability, reduced apoptosis, and decreased ROS in the HCN1-OE + PDTC group. Immunofluorescence and WB analysis indicated a reduction in HCN1 and NF-κB pathway protein levels in the HCN1-OE + PDTC group. In conclusion, PDTC provided protection against HCN1-induced cardiomyocyte injury, potentially by modulating inflammatory cytokines and regulating the IKKβ/IκB/NF-κB signaling pathway.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"19 1","pages":"20220967"},"PeriodicalIF":1.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammation. 主要的臭氧化自体血液疗法通过抑制氧化应激和炎症促进成年大鼠脊髓损伤后的功能恢复。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2024-12-31 eCollection Date: 2024-01-01 DOI: 10.1515/biol-2022-1004

Liwei Xia, Yongming Sun, Yue Zhou, Qian Yang, Jianhan Huang, Dong Liu

{"title":"Major ozonated autohemotherapy promoted functional recovery following spinal cord injury in adult rats via the inhibition of oxidative stress and inflammation.","authors":"Liwei Xia, Yongming Sun, Yue Zhou, Qian Yang, Jianhan Huang, Dong Liu","doi":"10.1515/biol-2022-1004","DOIUrl":"https://doi.org/10.1515/biol-2022-1004","url":null,"abstract":"This study sought to explore the value of major ozonated autohemotherapy (MOA) as a treatment for spinal cord injury (SCI) in a rat model system. In total, 54 female Sprague-Dawley rats were randomized into sham-operated, SCI model, and MOA treatment groups. We found that relative to the SCI model group, rats that underwent MOA treatment exhibited improved locomotor scores on days 14, 21, and 28 after injury (p < 0.05) together with reduced residual urine on days 5, 7, 14, and 21 after injury (p < 0.05). MOA treatment also lowered proinflammatory TNF-α, IL-1α, and C1q levels on day 3 post-injury (p < 0.05), decreased malondialdehyde levels, and enhanced superoxide dismutase activity (p < 0.001). Activated astrocytes in MOA-treated rats exhibited larger soma and higher levels of extracellular matrix secretion, whereas reactive microglia in the MOA group presented with a ramified morphology in contrast to the amoeboid morphology exhibited by these cells in SCI model rats. MOA offers potential value as a means of protecting spinal cord integrity, potentially through anti-inflammatory, antioxidant, and regulatory effects that shape the polarization of astrocytes and microglia.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"19 1","pages":"20221004"},"PeriodicalIF":1.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BA inhibits LPS-stimulated inflammatory response and apoptosis in human middle ear epithelial cells by regulating the Nf-Kb/Iκbα axis. BA通过调节Nf-Kb/ i - κbα轴抑制lps刺激的人中耳上皮细胞的炎症反应和凋亡。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2024-12-31 eCollection Date: 2024-01-01 DOI: 10.1515/biol-2022-1019

Qian He, Yanzhi Cai, Meihua Kong

{"title":"BA inhibits LPS-stimulated inflammatory response and apoptosis in human middle ear epithelial cells by regulating the Nf-Kb/Iκbα axis.","authors":"Qian He, Yanzhi Cai, Meihua Kong","doi":"10.1515/biol-2022-1019","DOIUrl":"10.1515/biol-2022-1019","url":null,"abstract":"Otitis media (OM) is a prevalent childhood ear disease characterized by inflammation of the middle ear cavity, which can lead to ear pain, fever, and hearing loss. The pathogenesis of OM is multifaceted, encompassing a variety of factors including bacterial or viral infections, host immune responses, and the function of middle ear epithelial cells. Boswellic acid (BA), a natural triterpene compound extracted from frankincense resin, has been proven to possess significant anti-inflammatory and immunomodulatory effects. This study aims to investigate the effects of BA on lipopolysaccharide (LPS)-stimulated inflammatory responses and apoptosis in human middle ear epithelial cells (HMEECs) and its potential mechanisms. Our findings demonstrated that BA enhances the proliferation of LPS-stimulated HMEECs and concurrently inhibits their apoptosis. In addition, BA blocked LPS-stimulated HMEEC inflammation. Mechanistically, BA suppressed the NF-κB/IκBα axis in LPS-stimulated HMEECs. In conclusion, BA effectively inhibits LPS-stimulated inflammation and apoptosis by mediating the NF-κB/IκBα axis, highlighting its potential as a therapeutic agent for OM.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"19 1","pages":"20221019"},"PeriodicalIF":1.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies. 大麻种子中酚类化合物的表征和抗糖尿病潜能的评价：体内、体外和计算机研究。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2024-12-31 eCollection Date: 2024-01-01 DOI: 10.1515/biol-2022-1024

Rafik El-Mernissi, Naoual El Menyiy, Amira Metouekel, Aziz Zouhri, Yahya El-Mernissi, Farhan Siddique, Sumaira Nadeem, Hassan Amhamdi, Oualid Abboussi, Abdulaziz Abdullah Alsahli, Mohammed Bourhia, Musaab Dauelbait, Gamal A Shazly, Lhoussain Hajji

{"title":"Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies.","authors":"Rafik El-Mernissi, Naoual El Menyiy, Amira Metouekel, Aziz Zouhri, Yahya El-Mernissi, Farhan Siddique, Sumaira Nadeem, Hassan Amhamdi, Oualid Abboussi, Abdulaziz Abdullah Alsahli, Mohammed Bourhia, Musaab Dauelbait, Gamal A Shazly, Lhoussain Hajji","doi":"10.1515/biol-2022-1024","DOIUrl":"10.1515/biol-2022-1024","url":null,"abstract":"Moroccan Cannabis sativa L. seeds were investigated for their phenolic profile and antidiabetic potential. Ultra-high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry analysis revealed a rich phenolic composition, including benzoic acid, cannabisin B, genistein, and epicatechin. In vitro, the seed extract exhibited potent α-amylase inhibitory activity (half-maximal inhibitory concentration = 25.02 ± 4.03 μg/mL). In vivo studies in diabetic rats demonstrated significant hypoglycemic, hypolipidemic, hepatoprotective, and nephroprotective effects. Molecular docking studies further supported these findings, revealing strong interactions between identified phenolic and the α-amylase enzyme. These results highlight the potential of C. sativa seeds as a natural source of bioactive compounds for diabetes management.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"19 1","pages":"20221024"},"PeriodicalIF":1.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of SWCNT-conjugated senna leaf extract on breast cancer cells: A potential apoptotic therapeutic strategy. swcnts结合的泻泻叶提取物对乳腺癌细胞的影响：一种潜在的凋亡治疗策略。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2024-12-31 eCollection Date: 2024-01-01 DOI: 10.1515/biol-2022-0994

Sabreen Mohammed Behairy, Saleh Mohammed Al-Maaqar, Majed Ahmed Al-Shaeri

{"title":"Impact of SWCNT-conjugated senna leaf extract on breast cancer cells: A potential apoptotic therapeutic strategy.","authors":"Sabreen Mohammed Behairy, Saleh Mohammed Al-Maaqar, Majed Ahmed Al-Shaeri","doi":"10.1515/biol-2022-0994","DOIUrl":"https://doi.org/10.1515/biol-2022-0994","url":null,"abstract":"Breast cancer (BC) has a prevalence rate of 21.8% among Saudi women and ranks as the third leading cause of death in Western nations. Nanotechnology offers innovative methods for targeted BC therapy, and this study explores the use of single-walled carbon nanotubes (SWCNTs) for delivering the senna leaf extract. The study evaluated the effects of increasing dosages of senna leaf extract conjugated to SWCNTs on MCF-7 cells. Cell viability was assessed using the MTT assay, while Giemsa staining revealed morphological changes. Additionally, the comet assay and agarose gel electrophoresis were employed to evaluate the pro-apoptotic potential. The potential of mitochondrial membrane and the production of reactive oxygen species (ROS) were investigated using the JC-1 dye. The results indicated that treated cells exhibited apoptotic characteristics, including elevated ROS levels and decreased mitochondrial membrane potential. In summary, the application of nanotechnology to deliver the senna leaf extract shows promise as a herbal treatment for BC, suggesting a potential breakthrough in combating this widespread and deadly disease.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"19 1","pages":"20220994"},"PeriodicalIF":1.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Axodendritic targeting of TAU and MAP2 and microtubule polarization in iPSC-derived versus SH-SY5Y-derived human neurons. ipsc衍生与sh - sy5y衍生人类神经元中TAU和MAP2的轴突靶向和微管极化。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2024-12-31 eCollection Date: 2024-01-01 DOI: 10.1515/biol-2022-1010

Helen Breuer, Michael Bell-Simons, Hans Zempel

{"title":"Axodendritic targeting of TAU and MAP2 and microtubule polarization in iPSC-derived versus SH-SY5Y-derived human neurons.","authors":"Helen Breuer, Michael Bell-Simons, Hans Zempel","doi":"10.1515/biol-2022-1010","DOIUrl":"https://doi.org/10.1515/biol-2022-1010","url":null,"abstract":"Cell polarity is crucial in neurons, characterized by distinct axonal and dendritic structures. Neurons generally have one long axon and multiple shorter dendrites, marked by specific microtubule (MT)-associated proteins, e.g., MAP2 for dendrites and TAU for axons, while the scaffolding proteins AnkG and TRIM46 mark the axon-initial-segment. In tauopathies, such as Alzheimer's disease (AD), TAU sorting, and neuronal polarity are disrupted, leading to MT loss. However, modeling and studying MTs in human neuronal cells relevant to the study of AD and TAU-related neurodegenerative diseases (NDD) is challenging. To study MT dynamics in human neurons, we compared two cell culture systems: SH-SY5Y-derived neurons (SHN) and induced pluripotent stem cell-derived neurons (iN). Using immunostaining and EB3-tdTomato time-lapse imaging, we found AnkG absent in SHN but present in iN, while TRIM46 was present in both. TAU and MAP2 showed axonal and dendritic enrichment, respectively, similar to mouse primary neurons. Both neuron types exhibited polarized MT structures, with unidirectional MTs in axons and bidirectional MTs in dendrites. Polymerization speeds were similar; however, iNs had more retrograde MT growth events, while SHN showed a higher overall number of growth events. Thus, SHN and iN are both suitable for studying neuronal cell polarity, with SHN being particularly suitable if the focus is not the AIS.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"19 1","pages":"20221010"},"PeriodicalIF":1.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of BCAA degradation is a critical characteristic of diabetic cardiomyopathy revealed by integrated transcriptome and metabolome analysis. 综合转录组和代谢组分析显示，BCAA降解的中断是糖尿病性心肌病的一个关键特征。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2024-12-31 eCollection Date: 2024-01-01 DOI: 10.1515/biol-2022-0974

Yanxia Wu, Wanxiang Jiang, Junlong Wang, Guoqing Xie, Yan Sun, Jinliang Yang

引用次数: 0

Baicalein inhibits NLRP3 inflammasome activation and mitigates placental inflammation and oxidative stress in gestational diabetes mellitus. 黄芩素抑制NLRP3炎性体激活，减轻妊娠期糖尿病胎盘炎症和氧化应激。

IF 1.7 4区生物学

Open Life Sciences Pub Date : 2024-12-31 eCollection Date: 2024-01-01 DOI: 10.1515/biol-2022-0966

Jun Yao, Jiaying Pan, Qiaoying Jiang, Hui Wang, Yiqi Zhao

{"title":"Baicalein inhibits NLRP3 inflammasome activation and mitigates placental inflammation and oxidative stress in gestational diabetes mellitus.","authors":"Jun Yao, Jiaying Pan, Qiaoying Jiang, Hui Wang, Yiqi Zhao","doi":"10.1515/biol-2022-0966","DOIUrl":"https://doi.org/10.1515/biol-2022-0966","url":null,"abstract":"Gestational diabetes mellitus (GDM) is a common metabolic disorder during pregnancy characterized by glucose intolerance, which poses risks to both maternal and fetal health. Baicalein, a flavonoid derived from the roots of Scutellaria baicalensis Georgi, exhibits various biological functions and has been implicated in the modulation of several diseases. However, the regulatory effects and underlying mechanisms of Baicalein in GDM progression remain unclear. In this study, we found that Baicalein ameliorates metabolic disturbances in GDM mice by improving glucose tolerance, insulin sensitivity, fasting blood glucose levels, and plasma insulin levels. Additionally, Baicalein treatment positively impacted litter size and birth weight. GDM mice exhibited increased inflammation and oxidative stress, which were mitigated following Baicalein administration (40 mg/kg). Furthermore, elevated protein levels of NLRP3, IL-1β, and IL-18 observed in GDM mice were reduced by Baicalein treatment. In conclusion, Baicalein inhibits the NLRP3 inflammasome and alleviates placental inflammation and oxidative stress associated with GDM. These findings provide valuable insights into the potential therapeutic role of Baicalein in managing GDM.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"19 1","pages":"20220966"},"PeriodicalIF":1.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0