Open Life Sciences最新文献_第2页

MGAT5 regulates ferroptosis via the Nrf2/HO-1 signaling pathway in diabetic nephropathy. MGAT5通过Nrf2/HO-1信号通路调节糖尿病肾病中的铁下垂。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-09-08 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1362

Lina Wang, Xiangjun Meng, Meng Wang, Lingbo Guan

{"title":"MGAT5 regulates ferroptosis via the Nrf2/HO-1 signaling pathway in diabetic nephropathy.","authors":"Lina Wang, Xiangjun Meng, Meng Wang, Lingbo Guan","doi":"10.1515/biol-2025-1362","DOIUrl":"10.1515/biol-2025-1362","url":null,"abstract":"Diabetic nephropathy (DN) is a major complication of diabetes mellitus, and identifying effective therapeutic targets remains an urgent need. In this study, genes associated with DN were obtained from the gene expression omnibus (GEO) database, and Bioinformatics analysis revealed that mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (MGAT5) was downregulated in DN. Functional experiments demonstrated that MGAT5 overexpression activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway, enhanced DN-related cell viability, and reduced the levels of lactate dehydrogenase (LDH), cleaved caspase-3 (C-casp3), Bcl-2-associated X protein (Bax), interleukin-6 (IL-6), interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), acyl-CoA synthetase long-chain family member 4 (ACSL4), prostaglandin-endoperoxide synthase 2/cyclooxygenase-2 (PTGS2/COX-2), Fe2+ content, ROS production, and apoptosis. Meanwhile, MGAT5 overexpression increased the levels of B-cell lymphoma 2 (Bcl-2), glutathione (GSH), and glutathione peroxidase 4 (GPX4). Conversely, MGAT5 knockdown partially reversed these protective effects. These findings suggest that MGAT5 may alleviate DN-associated cellular injury by activating the Nrf2/HO-1 pathway and regulating oxidative stress, inflammatory responses, ferroptosis, and apoptosis. MGAT5 may represent a potential therapeutic candidate for further investigation in diabetic nephropathy.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251362"},"PeriodicalIF":2.3,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13552620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms by which carbamoylated high-density lipoprotein (C-HDL) promotes calcific aortic valve disease and exploration of potential targeted therapies. 氨基甲酰化高密度脂蛋白（C-HDL）促进钙化主动脉瓣疾病的机制及潜在靶向治疗的探索

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-09-08 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1367

Min Han, Wang Jiang, Yalong Yang, Ran Chen

{"title":"Mechanisms by which carbamoylated high-density lipoprotein (C-HDL) promotes calcific aortic valve disease and exploration of potential targeted therapies.","authors":"Min Han, Wang Jiang, Yalong Yang, Ran Chen","doi":"10.1515/biol-2025-1367","DOIUrl":"10.1515/biol-2025-1367","url":null,"abstract":"Calcific aortic valve disease (CAVD) is a progressive fibrocalcific illness for which no effective pharmaceutical treatment exists. This study investigated whether carbamoylated high-density lipoprotein (C-HDL), a defective type of HDL that can develop during inflammation, contributes to CAVD progression and the involved molecular pathways. Male ApoE-/- mice were divided into three groups: CAVD model, cyanate-treated, and inhibitor, and analyzed after 12 weeks. C57BL/6 mice on a regular diet served as blank controls. Serum paraoxonase-1 (PON1), aortic valve calcification, cluster of differentiation 31 (CD31), phosphorylated nuclear factor kappa B p65 (p-p65), NOTCH receptor 1 (NOTCH1), and runt-related transcription factor 2 (RUNX2) were evaluated. In parallel, using RNA sequencing (RNA-seq), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, protein-protein interaction (PPI) network analysis, and quantitative real-time polymerase chain reaction. Cyanate treatment reduced serum PON1 levels, increased von Kossa-positive calcium deposition, and raised CD31, p-p65, NOTCH1, and RUNX2 levels compared with the model group, but Gly partially corrected these effects. Transcriptomic research identified 270 C-HDL-associated differentially expressed genes (DEGs) enriched in pathways associated with inflammatory signaling and NF-κB activity. Five potential hub genes (BIRC6, PIK3R1, ATM, IFIH1, and DDX58) were discovered and verified using qRT-PCR. These data show that C-HDL may accelerate CAVD by disrupting valve endothelial homeostasis and stimulating inflammatory signaling, and they identify potential molecular targets for future functional validation.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251367"},"PeriodicalIF":2.3,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13552616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress for RAD18's mechanism in breast cancer radioresistant. RAD18在乳腺癌耐药机制的研究进展。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-09-08 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1356

Yiming Zhao, Yuanhua Liu, Yiyang Yu, Lili Cao, Yanbin Xu

{"title":"Progress for RAD18's mechanism in breast cancer radioresistant.","authors":"Yiming Zhao, Yuanhua Liu, Yiyang Yu, Lili Cao, Yanbin Xu","doi":"10.1515/biol-2025-1356","DOIUrl":"10.1515/biol-2025-1356","url":null,"abstract":"Radioresistance remains a critical barrier to achieving durable locoregional control and improved survival outcomes in breast cancer. RAD18, an E3 ubiquitin ligase that regulates translesion synthesis and post-replication repair, has emerged as a candidate mediator of this phenotype by promoting proliferating cell nuclear antigen (PCNA) ubiquitination, facilitating replication stress tolerance, and enabling tumor cell survival following DNA damage. In parallel, the PI3K/AKT/mTOR (PAM) signaling pathway represents one of the most frequently dysregulated oncogenic networks in breast cancer and is increasingly recognized as a central determinant of therapeutic resistance. Notably, however, its biological and clinical relevance is highly context-dependent, with the strongest evidence observed in hormone receptor-positive (HR+)/HER2-negative disease and a more heterogeneous, less well-defined role in triple-negative breast cancer. In this narrative review, we integrate current evidence to examine the role of RAD18 in breast cancer radioresistance and to evaluate how RAD18-mediated DNA damage tolerance may be functionally embedded within broader PAM-driven survival programs. While existing data support a biologically plausible convergence between DNA damage tolerance mechanisms and oncogenic survival signaling, direct mechanistic evidence linking RAD18 to PAM pathway activity in breast cancer remains limited. We therefore propose a subtype-informed conceptual framework in which RAD18-dependent stress tolerance operates within a permissive survival signaling context. Such a framework may refine the interpretation of radioresistance and inform the development of predictive biomarkers as well as rational radiosensitization strategies.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251356"},"PeriodicalIF":2.3,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13552532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transrectal ultrasonographic parameters combined with total serum exosomes for the assessment of bone metastases in prostate cancer. 经直肠超声参数联合血清总外泌体评估前列腺癌骨转移。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-09-04 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1358

Lingxu Cui, Hua Hong, Guohua Wu, Qian Liu

{"title":"Transrectal ultrasonographic parameters combined with total serum exosomes for the assessment of bone metastases in prostate cancer.","authors":"Lingxu Cui, Hua Hong, Guohua Wu, Qian Liu","doi":"10.1515/biol-2025-1358","DOIUrl":"10.1515/biol-2025-1358","url":null,"abstract":"This study investigated transrectal ultrasound parameters and total serum exosome levels in prostate cancer (PCa) patients with bone metastasis (BM), and evaluated their combined value in assessing metastatic status. 40 PCa patients were categorized into non-bone metastasis (NBM, 21 cases) and BM (19 cases) groups based on single photon emission computed tomography/computed tomography or bone scans. Serum exosomes were extracted and analyzed via nanoparticle tracking analysis, while transrectal ultrasound and ultrasound-guided biopsies were performed. The study compared exosome levels and ultrasound parameters between groups, and assessed their diagnostic value for BM using receiver operating characteristic (ROC) curves, and analyzed correlations between exosomes and ultrasound findings. PCa patients with BM showed significantly higher serum exosome concentrations and ultrasound parameters (peak intensity, area under the curve (AUC), and Grad) than NBM patients (p < 0.001). ROC analysis revealed optimal cutoff values for discriminating BM: 19.33 dB for PI, 1,156.63 dB s for AUC, 2.02 dB/s for Grad, and 3.34 × E10 particles/mL for exosomes. The combined test (AUC = 0.97) outperformed individual parameters (AUCs 0.83-0.89), demonstrating superior diagnostic performance in identifying PCa BM. In conclusion, this exploratory preliminary study indicates that exosomes may have potential application value in the assessment of PCa BM.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251358"},"PeriodicalIF":2.3,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148897678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ocean acidification drives species-specific and time-dependent pigment responses in three phytoplankton taxa. 海洋酸化驱动三种浮游植物分类群的物种特异性和时间依赖性色素反应。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-09-03 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1374

Emel Kocaman, Koray Ozhan, Mustafa Mantikci

{"title":"Ocean acidification drives species-specific and time-dependent pigment responses in three phytoplankton taxa.","authors":"Emel Kocaman, Koray Ozhan, Mustafa Mantikci","doi":"10.1515/biol-2025-1374","DOIUrl":"10.1515/biol-2025-1374","url":null,"abstract":"Phytoplankton are primary producers in marine ecosystems and play a central role in biogeochemical cycles, yet their physiological responses to altered seawater pH vary among taxa and over time. Here, we examined the effects of sustained pH manipulation on pigment composition, growth, and dark respiration in three ecologically important phytoplankton taxa - a diatom (Pseudo-nitzschia spp.), a dinoflagellate (Heterocapsa pygmaea), and a haptophyte (Emiliania huxleyi) - during a 15-day controlled laboratory experiment. Cultures were maintained at pH 8.1, 7.8, and 7.5, representing present-day and enhanced acidification conditions. Responses to pH were species-specific and strongly time-dependent. Growth and cell-specific respiration rates showed relatively small and often transient differences among pH treatments, suggesting short-term metabolic adjustment under altered pH. In contrast, pigment composition exhibited clearer and more consistent pH-related responses, primarily expressed as shifts in temporal patterns rather than uniform directional changes. In Pseudo-nitzschia spp. and H. pygmaea, several key pigments displayed pronounced pH-dependent trajectories, whereas E. huxleyi showed greater temporal variability and weaker separation among pH treatments. Overall, these results demonstrate that photophysiological traits respond sensitively to sustained pH changes even when population-level growth and respiration remain comparatively stable, highlighting pigment composition as a potentially sensitive indicator of short-term phytoplankton acclimation to ocean acidification.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251374"},"PeriodicalIF":2.3,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13540600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnolol exerts anti-inflammatory effect in the animal model of rheumatoid arthritis. 厚朴酚在类风湿关节炎动物模型中具有抗炎作用。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-09-03 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1352

Guifu Qin, Siqi Li, Chong Chen, Yalong Qin, Zhiqin Ye, Tingting Pen, Qin Zhuo, Yuan Liang

{"title":"Magnolol exerts anti-inflammatory effect in the animal model of rheumatoid arthritis.","authors":"Guifu Qin, Siqi Li, Chong Chen, Yalong Qin, Zhiqin Ye, Tingting Pen, Qin Zhuo, Yuan Liang","doi":"10.1515/biol-2025-1352","DOIUrl":"10.1515/biol-2025-1352","url":null,"abstract":"As a typical inflammatory arthritis, rheumatoid arthritis (RA) reduces the life quality of patients from all over the world. Magnolol has been reported to exert the anti-inflammation role in several diseases. Here, we aim to investigate the effect of magnolol on the inflammatory response in vivo and in vitro. Indicators like arthritic index and paw weight were measured after collagen-induced arthritis (CIA) induction and magnolol treatment. The tissues of the rats' left hind paws were subjected to hematoxylin & eosin staining to observe the morphological changes. The serum inflammatory cytokines and MH7A cells were evaluated by enzyme-linked immunosorbent and real time quantitative polymerase chain reaction. Cell viability assay and flow cytometry were prepared for evaluating the viability and apoptosis of MH7A cells, respectively. Magnolol alleviated the paw inflammation and joint swelling in CIA rat model. CIA-induced upregulation of the serum inflammatory markers was also significantly reduced by magnolol in polyarthritis rat model. For in vitro experiments, magnolol treatment enhanced the viability and decreased apoptosis of tumor necrosis factor-alpha (TNF-α)-stimulated MH7A cells. Inflammatory cytokines were also downregulated by magnolol in TNF-α-stimulated MH7A cells. Magnolol alleviated RA development by inhibiting inflammatory response in CIA-induced rat model or TNF-α-treated MH7A cells.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251352"},"PeriodicalIF":2.3,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13540601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating context-specific acoustic variation in the bleats of male domestic sheep. 调查雄性家羊咩咩声的环境特异性声学变化。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1368

Apostolos Ntairis, Anastasia Frantzola, Ioannis Mavrikos, George P Laliotis

{"title":"Investigating context-specific acoustic variation in the bleats of male domestic sheep.","authors":"Apostolos Ntairis, Anastasia Frantzola, Ioannis Mavrikos, George P Laliotis","doi":"10.1515/biol-2025-1368","DOIUrl":"10.1515/biol-2025-1368","url":null,"abstract":"Vocalizations provide an informative means of assessing the emotional states of animals, yet male vocal behaviour in domestic sheep remains unexplored. The study aimed to investigate whether rams' bleats vary across five distinct contexts associated with morning and night isolation, exposure to familiar ewe-bell sounds, feed anticipation, and feed denial. A total of 428 high-quality bleats were used to extract 20 acoustic parameters, which were then analyzed using a multivariate acoustic approach. Principal Component Analysis identified five independent acoustic components reflecting source-parameters, spectral energy, formant frequencies, voice stability, and temporal characteristics. Context significantly influenced the acoustic structure of bleats, with spectral, formant, vocal stability, and temporal characteristics showing context-dependent variation, whereas fundamental frequency-related parameters remained unaffected. Feed anticipation elicited higher formant-related scores; feed denial produced reduced spectral energy, and social isolation contexts were characterized by increased vocal instability. Discriminant analyses revealed moderate classification accuracy, indicating overlapping acoustic patterns and supporting graded rather than discrete vocal signals. Morphometric effects were minimal, with wither height affecting formant structure. The findings provide the first detailed evidence that rams modulate multiple acoustic features across contexts, demonstrating that male sheep encode emotional states through shifts in vocal parameter variation.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251368"},"PeriodicalIF":2.3,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148887232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myeloid sarcoma as EM blast crisis in BCR-ABL1 e6a2+ CML with myelofibrosis and TB: a case report. 髓系肉瘤作为EM细胞危象在BCR-ABL1 e6a2+ CML伴骨髓纤维化和TB: 1例报告。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1369

Chunling Gu, Rui Lu, Yixin Kang, Wen Chen, Yongqing Zhang, Sainan Yang

{"title":"Myeloid sarcoma as EM blast crisis in BCR-ABL1 e6a2+ CML with myelofibrosis and TB: a case report.","authors":"Chunling Gu, Rui Lu, Yixin Kang, Wen Chen, Yongqing Zhang, Sainan Yang","doi":"10.1515/biol-2025-1369","DOIUrl":"10.1515/biol-2025-1369","url":null,"abstract":"BCR-ABL1 e6a2-positive chronic myeloid leukemia (CML) is a rare molecular subtype of CML, accounting for less than 1 % of CML cases. It was shown to be associated with aggressive clinical behavior and a tendency toward disease progression. Here, we report a 56-year-old man who presented with pancytopenia, cervical lymphadenopathy, and severe myelofibrosis, without the typical leukocytosis observed in classic CML. Concurrent active pulmonary tuberculosis further complicated the diagnostic process. Hyperuricemia and elevated levels of lactate dehydrogenase (LDH) suggested a high cell-turnover state, and cervical lymph node biopsy confirmed myeloid sarcoma. Cytogenetic analysis revealed t(9;22)(q34;q11.2) with trisomy 8. Real-time quantitative polymerase chain reaction (RT-qPCR) identified the rare BCR-ABL1 e6a2 transcript. The patient received second- and third-generation tyrosine kinase inhibitor (TKI)-based therapy combined with chemotherapy. An individualized rifamycin-sparing anti-tuberculosis regimen was also prescribed. The patient achieved complete remission with marked regression of myelofibrosis. This case suggests that e6a2-positive CML may present with atypical features, including pancytopenia, severe myelofibrosis, and extramedullary blast crisis. In patients with concurrent tuberculosis, timely tissue biopsy and molecular testing are essential to accelerate diagnosis.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251369"},"PeriodicalIF":2.3,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13524128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148851223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AtZIK4, a MAPK kinase kinase, positively regulates phosphate uptake gene expression to modulate phosphate starvation tolerance in Arabidopsis. AtZIK4是一种MAPK激酶，通过正向调控磷酸摄取基因表达来调节拟南芥的磷酸饥饿耐受性。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-08-27 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1361

Hongying Deng, Qian Yang, Shirong Zhao, Xuyang Lei, Liye Zhu

{"title":"AtZIK4, a MAPK kinase kinase, positively regulates phosphate uptake gene expression to modulate phosphate starvation tolerance in Arabidopsis.","authors":"Hongying Deng, Qian Yang, Shirong Zhao, Xuyang Lei, Liye Zhu","doi":"10.1515/biol-2025-1361","DOIUrl":"10.1515/biol-2025-1361","url":null,"abstract":"The mitogen-activated protein kinase (MAPK) cascade is a highly conserved signal transduction module in eukaryotes. We investigated the function of ZIK4, a MAPK kinase, in the phosphate starvation response of Arabidopsis. Phenotypic analysis revealed that the zik4 mutant had stronger tolerance to phosphate starvation than that to the wild type. However, the kinase domain was still expressed in zik4. The CRISPR/Cas9 system was used to construct two independent knockout mutants zik4-c3 and zik4-c15 by targeting two different sites in the AtZIK4 kinase domain. Physiological measurements showed that the phosphate content in zik4-c3 and zik4-c15 was lower than that in the wild type. Expression pattern analysis revealed that ZIK4 was induced to express under phosphate starvation. Quantitative real-time PCR results showed that the expression levels of multiple key phosphate uptake-related genes were significantly up-regulated in the zik4 mutant. These results indicate that ZIK4, as a phosphate starvation-induced MAPKKK, promotes phosphate accumulation in Arabidopsis seedlings by upregulating the expression levels of phosphate uptake-related genes. These findings not only elucidate the regulatory role of AtZIK4 kinase during abiotic stress and expand the low-phosphate (LP) signaling cascade in Arabidopsis thaliana, but also providing promising genetic targets to enhance crop performance under phosphorus-limited conditions.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251361"},"PeriodicalIF":2.3,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13512907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148831200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Schisandrin A alleviates allergic rhinitis by restoring Th1/Th2 and Treg/Th17 immune balance. 五味子素A通过恢复Th1/Th2和Treg/Th17免疫平衡缓解变应性鼻炎。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-08-25 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1348

Zhipeng Zhang, Shan He, Yanmin Yu, Yongjun Deng, Yuan Chen, Xiaoyi Gu, Xiaoyi Qiu, Jingjing Wei, Yan Shi, Yu Jiao

{"title":"Schisandrin A alleviates allergic rhinitis by restoring Th1/Th2 and Treg/Th17 immune balance.","authors":"Zhipeng Zhang, Shan He, Yanmin Yu, Yongjun Deng, Yuan Chen, Xiaoyi Gu, Xiaoyi Qiu, Jingjing Wei, Yan Shi, Yu Jiao","doi":"10.1515/biol-2025-1348","DOIUrl":"10.1515/biol-2025-1348","url":null,"abstract":"Allergic rhinitis (AR) is a common chronic inflammatory disorder characterized by nasal mucosal inflammation, sneezing, and rhinorrhea. Schisandrin A (Schis-A) has demonstrated significant anti-inflammatory effects in models of allergic asthma. This study aimed to evaluate the therapeutic efficacy of Schis-A in AR and to elucidate its underlying molecular mechanisms. An AR mouse model was established by sensitization and challenge with ovalbumin (OVA), followed by oral administration of Schis-A at doses of 40 and 80 mg/kg/day. Nasal symptoms were monitored and quantified. OVA-specific immunoglobulin E (IgE) levels and T helper (Th)1/Th2- and regulatory T cells (Treg)/Th17-associated cytokines were measured using enzyme-linked immunosorbent assay and quantitative real-time PCR. Treg and Th17 cell populations were analyzed by flow cytometry. The results showed that Schis-A treatment significantly reduced OVA-induced nasal rubbing and sneezing, indicating effective attenuation of allergic responses. Serum OVA-specific IgE levels were markedly reduced in Schis-A-treated AR mice. Moreover, Schis-A restored Th1/Th2 and Treg/Th17 cytokine balance, as indicated by upregulated expression of IFN-γ, IL-2, and Foxp3, alongside downregulation of IL-4, IL-5, IL-13, and IL-17. Flow cytometric analysis confirmed an increase in Treg cells and a reduction in Th17 cell populations. Mechanistically, Schis-A inhibited activation of the PI3K/AKT signaling pathway, suggesting that this pathway may contribute to its immunomodulatory effects. Schis-A alleviates AR by modulating Th1/Th2 and Treg/Th17 immune responses and inhibiting PI3K/AKT pathway activation, offering a promising therapeutic strategy for the treatment of AR.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251348"},"PeriodicalIF":2.3,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13527562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0